MC03g_new0287 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC03g_new0287
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	berberine bridge enzyme-like 28
Location	MC03: 12855314 .. 12858595 (-)
RNA-Seq Expression	MC03g_new0287
Synteny	MC03g_new0287

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AAGGGACTTTATATAAACTTGTACTACTCCTCTGATTGCGAATTGATTTTAATATGAAATTTCATGAATATTAATAAAAAAAAAGTCTCAAATTTACCCCAAGGTAAATGTTAGGACATATTTTTCTCACCAGACAAATATTTCATATATCAATTATGTATATCATAGATTTTTTCCTCAATGGGACAATGTTCACCATAAATAATGAAGCTTCCAAATGCATAGATCAAATCTCCAAACCGGCGATGAAGTACTCTTCTCCATTAATCTCTCTTTGTCTATCTCTTGCCTTTATCCTTCTATCTTCATCTCCATTATGGGCTCACAAAAATGAAGAATATTTTATTCCATGTCTCTCCAATCACTCTCTAGATGGTTACTCCATTTTCAATGTTGTTTACGACTCCGCCTATTCTTCTATCTTGAATTTCTCGGTTCGAAATCCTAGATTCTCAACTATTGACACCCCAAAGCCACTCTTCATCATCACACCATCACATATTTCCCACATTCAAGCAGCCATCATTTGCTCCAAAACCCATGGCCTTCAAATCCGAACTCGAAGCGGCGGCCATGACTACGAGGGTCTTTCCTACGTCTCCCACCTTCCATTCATAATCGTTGACCTAATAAATATGAGGTCTATCACTATTGACGTTAAAGACAAAACTACATGGGTTCAGTCCGGGCCAACTCTCGGTGAACTCTACTATAGAATAGCTAAGAAAAGTCGAACCCTAGCGTTTCCGGCGGGTATTTGCCCGACAGTTGGCGTTGGCGGACACTTTAGTGGTGGTGGTATGGGTTGATGATGAGAAAATATGGTCTTGCCGTTGATAATGTGATCGACGCTTACTTGATTGATGCTAATGGAAAATTTCACGATAGAGAATCGATGGGGGAAGATTTGTTTTGGGCTATTAGAGGGGGCGGCGGAGGAAGCTTCGGAAGCGTGGTGGTGTGGAAGGTGAAGCTGGTTTCGGTTCCGGGGACTGTAACGAGTTGCTCTAGTAACACAACTTTGGAGAAAGATGCAGTGAAGCTAATTCATTTGTGGCAACATGTTGCTCATACATTGGATGAAAATCTAGCCCTTAGCATCTATTTGTCTGGTATTCTTCAAATGGTTTATGCTTGAGTTATTTATTCATTTATCACTTTTGAGAATAAAATATTATTTCTTATGAATTGATAATGAAAGTGAAAAAGACATCCCACCAAATGGTTTTTTAAGAAACAAAAAAGATAAAAAGGTTTCATATTTTAATTTTCAGGAGCAAAGTTTGAACTTTTTCAGAAAAACTTTATCACATTTCCCACTAAACTTAGATTTAAATTTAATTTTTATTGGTGTTTGGTGGGTTTGGTATACTTAGTGAGATTGTGCTTTTAGCTAATAAAAATGGTTTGAAAATTATAAAAACATTTTCATTGAGGTTAGTACTTAATAGAAATGTATTTTACTTAAAAAGATTGCTTTCAAATAGCAATTTCAAATTTACTATGAATATTTATGCCAACATTATGATTTTATTTTTATTTTTATTTTTTGTCTAGACCTGGGTAATTTGCTGCTGTTTTTAAGTTGTTTTTTGTTTTCTTTTTGTTTTTTTTCCTCTTTTACGTTGAAATATATTGAAGCATTATTTTTGTTTAGTTGTGAACGTTCTCGTATTTAAATTTCAAGGATGTGTGTTAATTGGACTTTGAAGTTATTTTCAGGTTAATGATGAAATTAAAATTTTAAAGTTAAATAAGTTTTAGTCTTACTCTCTCTAAATCATGGTTTTGAAATTTTTAAATCTTTTACGATGGTCTTTGAACTTCTCATAAAAGAATATATATATATATATATATATTTTGTTATCGAGGTTAATTTTATTCTATAAATTATTCATCAAAAACTTTATACGAATGCATCTATTTATAATATATTTCTCTCTTCCTCCATCTCTTCTCGCAGTAGCTCTTCCTTTTTCCCACAGACACTGCCTTCCATTTTTAGTATGGTTCTTCAAGCGTAATCTCTGATCATCTTGTTTCTCTCTTCTCTTCGGTTTTGGCCGAAATCGATCGACTATTGATTTCGTTGTTGCTTTCAAAATTGCCAGACATCAACCAACATTAATTGAATGGTCTATTTTCAGCGTCAGTTTGTGATTAAAACCGATACCAACCGACCAATGATCAACCTTAAGTCACCCATCATCAATTATGTGGAACTAAATAAATTGAAAACTGGCCTAATTCTTTTTTCTTCTATCCTATTCTTAAAAACAAATAAAAAAAGGGTTTATCCTAAAATTTCGTATCCCAAATATATTGCATTAAACCACTAATATGAAAATTTAAATTCAATTCTTGATGATAGGTGGGATTTCTACTCAAGGAGGCAAAATAAATAAAAATCCAATAGTCTTATTCGTCACTTTCTTTCTTGGGAAGGCAGATGAACTTATAACAATTTTGAAGGAAAATTTCCCAGAGTGGGATTTGGCAAAGGAAGATTGCACAGAATCAAGTTGGATTGAATCAGCTCTCATAAATGCAAGACTGATAGGGTATCCAACTGATGAATCCTTAGAATCTTTGCTAAAAAGAGATACTCGTCCTCTCGCCGAAGCGAGCATCAAAATCAAATCTGGTTATGTCAAGGAACCCATTGCCGAGGCTGCAATCAAGAACATATGGGAAAGATTAAAAACTCGAGATAAAAGAAGGGAAGATTTTTATACTTATACCTTATGGAGGGAGAATGAGTCGTATTCCAGAGTCGGAAACTCCTTATGCGCACAGAGCTGGATATCTGTACAAAATTGGTTACAGTATCCAATGGGAAGAAAAAGGCATTGATGTAGAGAAAAGACACCTCAATTGGATACGAGGGTTTTATGATTACTTAGCTCCTTACGTTTCAAAATCTCCGAGAGCTGCGTATCTCAATTATAGAGACCTTGATATTGGAACCAACAACATATATGGAAAGATCAGCTACAAGCAAGCAAGCAATTGGGGATTAAAGTATTTTGGTAACAATTTTAAAAGGTTGGTACATGTTAAGACCAAAGTTGATCCTGACAATTTCTTTAGGCATGAACAAAGCGTACCTACTCTCTAGCGGTGAAAAAGTTGGAAACCACTCGCTCGCTGCTTGATAGTGACTTCTTTCGATATCTTTTTAAAGTTTGTTTAAACTATGCTCCAACCTATTTATGTAGCGAAATAAGAGGTTGTATGACTGCATGTATCTATACACTTTTTTTTTGATGTGCGTGTCTTGAT

mRNA sequence

ATGATGAGAAAATATGGTCTTGCCGTTGATAATGTGATCGACGCTTACTTGATTGATGCTAATGGAAAATTTCACGATAGAGAATCGATGGGGGAAGATTTGTTTTGGGCTATTAGAGGGGGCGGCGGAGGAAGCTTCGGAAGCGTGGTGGTGTGGAAGGTGAAGCTGGTTTCGGTTCCGGGGACTGTAACGAGTTGCTCTAGTAACACAACTTTGGAGAAAGATGCAGTGAAGCTAATTCATTTGTGGCAACATGTTGCTCATACATTGGATGAAAATCTAGCCCTTAGCATCTATTTGTCTGGTGGGATTTCTACTCAAGGAGGCAAAATAAATAAAAATCCAATAGTCTTATTCGTCACTTTCTTTCTTGGGAAGGCAGATGAACTTATAACAATTTTGAAGGAAAATTTCCCAGAGTGGGATTTGGCAAAGGAAGATTGCACAGAATCAAGTTGGATTGAATCAGCTCTCATAAATGCAAGACTGATAGGGTATCCAACTGATGAATCCTTAGAATCTTTGCTAAAAAGAGATACTCGTCCTCTCGCCGAAGCGAGCATCAAAATCAAATCTGGTTATGTCAAGGAACCCATTGCCGAGGCTGCAATCAAGAACATATGGGAAAGATTAAAAACTCGAGATAAAAGAAGGGAAGATTTTTATACTTATACCTTATGGAGGGAGAATGAGTCGTATTCCAGAGTCGGAAACTCCTTATGCGCACAGAGCTGGATATCTGTACAAAATTGGTTACAGTATCCAATGGGAAGAAAAAGGCATTGA

Coding sequence (CDS)

ATGATGAGAAAATATGGTCTTGCCGTTGATAATGTGATCGACGCTTACTTGATTGATGCTAATGGAAAATTTCACGATAGAGAATCGATGGGGGAAGATTTGTTTTGGGCTATTAGAGGGGGCGGCGGAGGAAGCTTCGGAAGCGTGGTGGTGTGGAAGGTGAAGCTGGTTTCGGTTCCGGGGACTGTAACGAGTTGCTCTAGTAACACAACTTTGGAGAAAGATGCAGTGAAGCTAATTCATTTGTGGCAACATGTTGCTCATACATTGGATGAAAATCTAGCCCTTAGCATCTATTTGTCTGGTGGGATTTCTACTCAAGGAGGCAAAATAAATAAAAATCCAATAGTCTTATTCGTCACTTTCTTTCTTGGGAAGGCAGATGAACTTATAACAATTTTGAAGGAAAATTTCCCAGAGTGGGATTTGGCAAAGGAAGATTGCACAGAATCAAGTTGGATTGAATCAGCTCTCATAAATGCAAGACTGATAGGGTATCCAACTGATGAATCCTTAGAATCTTTGCTAAAAAGAGATACTCGTCCTCTCGCCGAAGCGAGCATCAAAATCAAATCTGGTTATGTCAAGGAACCCATTGCCGAGGCTGCAATCAAGAACATATGGGAAAGATTAAAAACTCGAGATAAAAGAAGGGAAGATTTTTATACTTATACCTTATGGAGGGAGAATGAGTCGTATTCCAGAGTCGGAAACTCCTTATGCGCACAGAGCTGGATATCTGTACAAAATTGGTTACAGTATCCAATGGGAAGAAAAAGGCATTGA

Protein sequence

MMRKYGLAVDNVIDAYLIDANGKFHDRESMGEDLFWAIRGGGGGSFGSVVVWKVKLVSVPGTVTSCSSNTTLEKDAVKLIHLWQHVAHTLDENLALSIYLSGGISTQGGKINKNPIVLFVTFFLGKADELITILKENFPEWDLAKEDCTESSWIESALINARLIGYPTDESLESLLKRDTRPLAEASIKIKSGYVKEPIAEAAIKNIWERLKTRDKRREDFYTYTLWRENESYSRVGNSLCAQSWISVQNWLQYPMGRKRH

Homology

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BLAST of MC03g_new0287 vs. ExPASy Swiss-Prot
Match: A0A2S1XB67 (O-acetylstemmadenine oxidase OS=Catharanthus roseus OX=4058 GN=ASO PE=1 SV=1)

HSP 1 Score: 186.8 bits (473), Expect = 3.1e-46
Identity = 94/209 (44.98%), Postives = 135/209 (64.59%), Query Frame = 0


Query: 1   MMRKYGLAVDNVIDAYLIDANGKFHDRESMGEDLFWAIRGGGGGSFGSVVVWKVKLVSVP 60
           ++RKYGLA DN+IDA ++DA G+  DRESMGEDLFWAIRGGGG SFG +V WKVKLV VP
Sbjct: 186 LLRKYGLAADNIIDAKIVDARGRILDRESMGEDLFWAIRGGGGASFGVIVSWKVKLVKVP 245

Query: 61  GTVTSCSSNTTLEKDAVKLIHLWQHVAHTLDENLALSIYLSGGISTQGGKINKNPIVLFV 120
             VT    + T E+  + L+H WQ++ H L E+L L++     I       NK  +  F+
Sbjct: 246 PMVTVFILSKTYEEGGLDLLHKWQYIEHKLPEDLFLAV----SIMDDSSSGNKTLMAGFM 305

Query: 121 TFFLGKADELITILKENFPEWDLAKEDCTESSWIESALINARLIGYPTDESLESLLKRDT 180
           + FLGK ++L+ ++ ENFP+  L KEDC E +WI++A+      G+P  ES  S+LK   
Sbjct: 306 SLFLGKTEDLLKVMAENFPQLGLKKEDCLEMNWIDAAMY---FSGHPIGES-RSVLKNRE 365

Query: 181 RPLAEASIKIKSGYVKEPIAEAAIKNIWE 210
             L +  + IKS +++EP +  A++ +W+
Sbjct: 366 SHLPKTCVSIKSDFIQEPQSMDALEKLWK 386

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BLAST of MC03g_new0287 vs. ExPASy Swiss-Prot
Match: O64743 (Berberine bridge enzyme-like 15 OS=Arabidopsis thaliana OX=3702 GN=MEE23 PE=1 SV=1)

HSP 1 Score: 185.7 bits (470), Expect = 6.9e-46
Identity = 103/267 (38.58%), Postives = 159/267 (59.55%), Query Frame = 0


Query: 1   MMRKYGLAVDNVIDAYLIDANGKFHDRESMGEDLFWAIRGGGGGSFGSVVVWKVKLVSVP 60
           MMRK+GL  DNV+DA ++DANG+  DR +MGED+FWAIRGGGGGSFG ++ WK+KLV VP
Sbjct: 196 MMRKFGLGADNVLDARIVDANGQILDRAAMGEDVFWAIRGGGGGSFGVILAWKIKLVPVP 255

Query: 61  GTVTSCSSNTTLEKDAVKLIHLWQHVAHTLDENLALSIYLS-GGISTQGGKINKNPIVLF 120
            TVT  +   TLE+D  K+++ W+ +A  LD++L + + +S    +T+ G  N+   + +
Sbjct: 256 ATVTVFTVTKTLEQDGTKVLYKWEQIADKLDDDLFIRVIISPASKTTKPG--NRTISMSY 315

Query: 121 VTFFLGKADELITILKENFPEWDLAKEDCTESSWIESALINARLIGYPTDESLESLLKRD 180
              FLG ++ L+ +++++FPE  L K+DCTE SWI+S +    + G+P   + E+LL   
Sbjct: 316 QAQFLGDSNRLLQVMQKSFPELGLTKKDCTEMSWIKSVMY---IAGFPNSAAPEALLA-- 375

Query: 181 TRPLAEASIKIKSGYVKEPIAEAAIKNIWERLKTRDKRREDFYTY--TLWRENES---YS 240
            + L +   K KS +VKEPI    ++ +WER    D     +  Y   + R +ES   + 
Sbjct: 376 GKSLFKNHFKAKSDFVKEPIPVEGLEGLWERFLEEDSPLTIWNPYGGMMSRISESEIPFP 435

Query: 241 RVGNSLCAQSWISVQNWLQYPMGRKRH 262
               +L    W+S   W    +  +RH
Sbjct: 436 HRNGTLFKIQWLS--TWQDGKVSEERH 453

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BLAST of MC03g_new0287 vs. ExPASy Swiss-Prot
Match: Q93ZA3 (Berberine bridge enzyme-like 13 OS=Arabidopsis thaliana OX=3702 GN=At1g30760 PE=1 SV=1)

HSP 1 Score: 181.4 bits (459), Expect = 1.3e-44
Identity = 93/215 (43.26%), Postives = 136/215 (63.26%), Query Frame = 0


Query: 1   MMRKYGLAVDNVIDAYLIDANGKFHDRESMGEDLFWAIRGGGGGSFGSVVVWKVKLVSVP 60
           MMRK+GL  DNV+DA ++DA+GK  +R +MGED+FWAIRGGGGGSFG ++ WK+KLV VP
Sbjct: 198 MMRKFGLGADNVLDARIVDADGKILNRAAMGEDVFWAIRGGGGGSFGVILAWKIKLVPVP 257

Query: 61  GTVTSCSSNTTLEKDAVKLIHLWQHVAHTLDENLALSIYLSGGISTQGGKINKNPIVLFV 120
             VT  +   TLE+D  KL++ WQ VA  LDE+L + + +     T   K  +     + 
Sbjct: 258 EIVTVFTVTRTLEQDGTKLLYKWQQVADKLDEDLFIRVIIQPTSKTPKSK-ERTISTSYQ 317

Query: 121 TFFLGKADELITILKENFPEWDLAKEDCTESSWIESALINARLIGYPTDESLESLLKRDT 180
             FLG A+ L+ +++ +FP+  L K+DC E+SWI+S +    + G+P+    E+LL  D 
Sbjct: 318 GQFLGDANRLLQVMQRSFPQLGLTKKDCLETSWIKSVMY---IAGFPSTAPSEALL--DG 377

Query: 181 RPLAEASIKIKSGYVKEPIAEAAIKNIWERLKTRD 216
           + L +   K KS YV+EPI    ++ +WE+L   D
Sbjct: 378 KSLFKNYFKAKSDYVEEPIPVEGLEGLWEKLLEED 406

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BLAST of MC03g_new0287 vs. ExPASy Swiss-Prot
Match: Q9FI21 (Berberine bridge enzyme-like 28 OS=Arabidopsis thaliana OX=3702 GN=At5g44440 PE=1 SV=1)

HSP 1 Score: 181.4 bits (459), Expect = 1.3e-44
Identity = 98/218 (44.95%), Postives = 135/218 (61.93%), Query Frame = 0


Query: 1   MMRKYGLAVDNVIDAYLIDANGKFHDRESMGEDLFWAIRGGGGGSFGSVVVWKVKLVSVP 60
           ++RKYGLA DN+IDA ++DA+G+  DR++MGED FWAIRGGGG SFG ++ WKVKLV VP
Sbjct: 191 LLRKYGLAADNIIDALVVDASGRILDRQAMGEDYFWAIRGGGGSSFGVILSWKVKLVDVP 250

Query: 61  GTVTSCSSNTTLEKDAVKLIHLWQHVAHTLDENLALSIYLSGGISTQGGKINKNPI-VLF 120
            T+T      T +K+AV++I  WQ+ A  + ++L +   L         + NKN +  LF
Sbjct: 251 STITVFKVQKTSKKEAVRIIKKWQYAADKVPDDLFIRTTLE--------RSNKNAVHALF 310

Query: 121 VTFFLGKADELITILKENFPEWDLAKEDCTESSWIESALINARLIGYPTDESLESLLKRD 180
              ++G  + L+ +++E FPE  L KE C E SWIES L  A    +P  ESL  L  R+
Sbjct: 311 TGLYIGPVNNLLALMEEKFPELGLEKEGCEEMSWIESVLWFA---DFPKGESLGVLTNRE 370

Query: 181 TRPLAEASIKIKSGYVKEPIAEAAIKNIWERLKTRDKR 218
              L   S K K  +V+EPI EAAI+ IW RL+  + R
Sbjct: 371 RTSL---SFKGKDDFVQEPIPEAAIQEIWRRLEAPEAR 394

[Close]

BLAST of MC03g_new0287 vs. ExPASy Swiss-Prot
Match: Q9FKV0 (Berberine bridge enzyme-like 24 OS=Arabidopsis thaliana OX=3702 GN=At5g44380 PE=2 SV=1)

HSP 1 Score: 179.1 bits (453), Expect = 6.5e-44
Identity = 97/212 (45.75%), Postives = 135/212 (63.68%), Query Frame = 0


Query: 1   MMRKYGLAVDNVIDAYLIDANGKFHDRESMGEDLFWAIRGGGGGSFGSVVVWKVKLVSVP 60
           +MRKYGLA DNV+D  ++DANGK  DR +MGEDLFWAIRGGGG SFG V+ WK+KLV VP
Sbjct: 201 LMRKYGLAGDNVLDVKMVDANGKLLDRAAMGEDLFWAIRGGGGASFGIVLAWKIKLVPVP 260

Query: 61  GTVTSCSSNTTLEKDA-VKLIHLWQHVAHTLDENLALSIYLSGGISTQGGKINKNPIVLF 120
            TVT  +   TLE+DA +K I  WQ ++  + E + + + L       G   NK   + +
Sbjct: 261 KTVTVFTVTKTLEQDARLKTISKWQQISSKIIEEIHIRVVLRAA----GNDGNKTVTMTY 320

Query: 121 VTFFLGKADELITILKENFPEWDLAKEDCTESSWIESALINARLIGYPTDESLESLLKRD 180
           +  FLG+   L+ ++++ FPE  L ++DCTE SWIE+AL +    G+PT   +E LL+  
Sbjct: 321 LGQFLGEKGTLLKVMEKAFPELGLTQKDCTEMSWIEAALFHG---GFPTGSPIEILLQLK 380

Query: 181 TRPLAEASIKIKSGYVKEPIAEAAIKNIWERL 212
           + PL +   K  S +VKEPI    +K I++RL
Sbjct: 381 S-PLGKDYFKATSDFVKEPIPVIGLKGIFKRL 404

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BLAST of MC03g_new0287 vs. NCBI nr
Match: XP_022152424.1 (berberine bridge enzyme-like 28 [Momordica charantia])

HSP 1 Score: 334 bits (856), Expect = 1.34e-109
Identity = 170/224 (75.89%), Postives = 185/224 (82.59%), Query Frame = 0


Query: 1   MMRKYGLAVDNVIDAYLIDANGKFHDRESMGEDLFWAIRGGGGGSFGSVVVWKVKLVSVP 60
           MMRKYGLA DNVIDAYLIDANGKFHDRESMGEDLFWAIRGG GGSFG VV WKVKLV VP
Sbjct: 191 MMRKYGLAADNVIDAYLIDANGKFHDRESMGEDLFWAIRGGAGGSFGIVVAWKVKLVPVP 250

Query: 61  GTVTSCSSNTTLEKDAVKLIHLWQHVAHTLDENLALSIYLSGGI---STQGGKINKNPIV 120
            TVTSCS N TLEKDAVKLIH WQ+VAH LDENL L   LSGG+   S  GG+ NKNPI 
Sbjct: 251 ATVTSCSINRTLEKDAVKLIHRWQYVAHKLDENLFLGTILSGGVLNVSQGGGETNKNPIA 310

Query: 121 LFVTFFLGKADELITILKENFPEWDLAKEDCTESSWIESALINARLIGYPTDESLESLLK 180
           LF T FLGK DEL+ ILK NFPE  LAKEDC ESSWIESAL+NA+L G+P DESLE+LLK
Sbjct: 311 LFFTLFLGKVDELVKILKTNFPELVLAKEDCIESSWIESALVNAKLTGFPADESLETLLK 370

Query: 181 RDTRPLAEASIKIKSGYVKEPIAEAAIKNIWERLKTRDKRREDF 221
           RD  PL +AS+KIKS YVKEPI+E AI+ IWERLKT+DK+ + F
Sbjct: 371 RD--PLTKASMKIKSDYVKEPISETAIEGIWERLKTQDKQGKVF 412

[Close]

BLAST of MC03g_new0287 vs. NCBI nr
Match: XP_038895343.1 (cannabidiolic acid synthase-like [Benincasa hispida])

HSP 1 Score: 278 bits (712), Expect = 6.82e-87
Identity = 145/216 (67.13%), Postives = 170/216 (78.70%), Query Frame = 0


Query: 1   MMRKYGLAVDNVIDAYLIDANGKFHDRESMGEDLFWAIRGGGGGSFGSVVVWKVKLVSVP 60
           ++RKYGLA DNVIDAYL+DANG+FHDRESMGEDLFWAIRGGGGGSFG VV WKVKLV VP
Sbjct: 195 LLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVP 254

Query: 61  GTVTSCSSNTTLEKDAVKLIHLWQHVAHTLDENLALSIYLSGG-ISTQGGKINKNPIVLF 120
            TVT C++N T ++ A+KLI  WQ+VA+ LDE+L LSI L+GG IS QGGKIN  P  LF
Sbjct: 255 ATVTICTANRTWKEGAIKLIDQWQYVANKLDEDLFLSIILTGGKISAQGGKIN--PTALF 314

Query: 121 VTFFLGKADELITILKENFPEWDLAKEDCTESSWIESALINARLIGYPTDESLESLLKRD 180
            T FLGKADEL+TILK  FPE +LAKEDC E+SWIESA+  A  +    D+ LE+ L R 
Sbjct: 315 FTLFLGKADELLTILKNKFPELNLAKEDCKETSWIESAVYTANGLQID-DQPLEAFLNRT 374

Query: 181 TRPLAEASIKIKSGYVKEPIAEAAIKNIWERLKTRD 215
             PLA  S KIKS YVKEPI +AAI+ IW+RLK++D
Sbjct: 375 --PLANGSFKIKSDYVKEPITKAAIEGIWQRLKSQD 405

[Close]

BLAST of MC03g_new0287 vs. NCBI nr
Match: XP_022152516.1 (berberine bridge enzyme-like 18 [Momordica charantia])

HSP 1 Score: 276 bits (705), Expect = 7.22e-86
Identity = 143/215 (66.51%), Postives = 160/215 (74.42%), Query Frame = 0


Query: 1   MMRKYGLAVDNVIDAYLIDANGKFHDRESMGEDLFWAIRGGGGGSFGSVVVWKVKLVSVP 60
           M+RK+GLA DNVIDAYL+DANGKF DRE MGEDLFWAIRGGGGGSFG VV WKVKLV VP
Sbjct: 196 MLRKFGLAADNVIDAYLVDANGKFQDRELMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVP 255

Query: 61  GTVTSCSSNTTLEKDAVKLIHLWQHVAHTLDENLALSIYLSGGISTQGGKINKNPIVLFV 120
            TVTSC  N TLE+ A+KLIH WQ+VA+ LDENL L I L GG  T  G    NP   F 
Sbjct: 256 ATVTSCYYNRTLEEGAIKLIHRWQYVANKLDENLFLGILLMGGNFTAQGGDKTNPTASFF 315

Query: 121 TFFLGKADELITILKENFPEWDLAKEDCTESSWIESALINARLIGYPTDESLESLLKRDT 180
           + FLGKADE   IL   FPE  LAKE+C ESSWIESAL++A   G+   +SLE+LL R  
Sbjct: 316 SLFLGKADEFFAILNTTFPELGLAKENCLESSWIESALLSA---GFKIGDSLEALLNRT- 375

Query: 181 RPLAEASIKIKSGYVKEPIAEAAIKNIWERLKTRD 215
            PL   SIKIKS YVKEPI+EA I+ IWERLK+RD
Sbjct: 376 -PLTNGSIKIKSDYVKEPISEATIEGIWERLKSRD 405

[Close]

BLAST of MC03g_new0287 vs. NCBI nr
Match: XP_022142587.1 (tetrahydrocannabinolic acid synthase-like [Momordica charantia])

HSP 1 Score: 270 bits (689), Expect = 1.65e-83
Identity = 136/217 (62.67%), Postives = 167/217 (76.96%), Query Frame = 0


Query: 1   MMRKYGLAVDNVIDAYLIDANGKFHDRESMGEDLFWAIRGGGGGSFGSVVVWKVKLVSVP 60
           ++RKYGLA DNVIDAYL+DA+GKFH+RESMGEDLFWAIRGGGGGSFG VV WKVKLV VP
Sbjct: 195 LLRKYGLAADNVIDAYLVDASGKFHNRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVP 254

Query: 61  GTVTSCSSNTTLEKDAVKLIHLWQHVAHTLDENLALSIYLSGG--ISTQGGKINKNPIVL 120
            TVT CS+N TLE+ AVK+ H WQ+VA+ LDENL L I   GG   S +GGK   NP+  
Sbjct: 255 ATVTLCSANRTLEEGAVKITHRWQYVANKLDENLFLGILWLGGKITSQEGGK--TNPVAT 314

Query: 121 FVTFFLGKADELITILKENFPEWDLAKEDCTESSWIESALINARLIGYPTDESLESLLKR 180
           F + FLGKADEL++IL   FPE DLAK+DC E+SWIES ++    +G+PT+ +LE+LL R
Sbjct: 315 FFSLFLGKADELLSILNTKFPELDLAKKDCIETSWIESTVLMG--VGFPTELTLEALLSR 374

Query: 181 DTRPLAEASIKIKSGYVKEPIAEAAIKNIWERLKTRD 215
              PL   S KIKS YVKEP++EA ++ IW+RL ++D
Sbjct: 375 T--PLTNTSSKIKSDYVKEPMSEAVVQGIWKRLDSQD 405

[Close]

BLAST of MC03g_new0287 vs. NCBI nr
Match: XP_022152518.1 (berberine bridge enzyme-like 8 [Momordica charantia])

HSP 1 Score: 268 bits (685), Expect = 6.21e-83
Identity = 139/216 (64.35%), Postives = 161/216 (74.54%), Query Frame = 0


Query: 1   MMRKYGLAVDNVIDAYLIDANGKFHDRESMGEDLFWAIRGGGGGSFGSVVVWKVKLVSVP 60
           M+RKYGLA DNVIDAYLIDANGKF+DRESMGEDLFWAIRGGGGGSFG VV WKVKLV VP
Sbjct: 192 MLRKYGLAADNVIDAYLIDANGKFYDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVP 251

Query: 61  GTVTSCSSNTTLEKDAVKLIHLWQHVAHTLDENLALSIYLSGG-ISTQGGKINKNPIVLF 120
             VT+CSS+  L++D + LIH WQ VA+ LDENL   I L+GG  ST+GG    NP   F
Sbjct: 252 AIVTTCSSDRNLDEDTINLIHRWQSVAYKLDENLYFGIILAGGNASTEGGVKKPNPTATF 311

Query: 121 VTFFLGKADELITILKENFPEWDLAKEDCTESSWIESALINARLIGYPTDESLESLLKRD 180
            + FLGK+DEL+ IL  NFPE +LAK+DC E+SWIES L+ A      T ESLESLL R 
Sbjct: 312 FSLFLGKSDELMAILNTNFPELNLAKKDCVETSWIESTLVAAT--RNQTIESLESLLNRT 371

Query: 181 TRPLAEASIKIKSGYVKEPIAEAAIKNIWERLKTRD 215
             PL   + K+KS YVKEPI +A I+ IWERLK +D
Sbjct: 372 --PLTIENTKLKSDYVKEPIPKATIEGIWERLKAQD 403

[Close]

BLAST of MC03g_new0287 vs. ExPASy TrEMBL
Match: A0A6J1DFZ4 (berberine bridge enzyme-like 28 OS=Momordica charantia OX=3673 GN=LOC111020160 PE=4 SV=1)

HSP 1 Score: 334 bits (856), Expect = 6.49e-110
Identity = 170/224 (75.89%), Postives = 185/224 (82.59%), Query Frame = 0


Query: 1   MMRKYGLAVDNVIDAYLIDANGKFHDRESMGEDLFWAIRGGGGGSFGSVVVWKVKLVSVP 60
           MMRKYGLA DNVIDAYLIDANGKFHDRESMGEDLFWAIRGG GGSFG VV WKVKLV VP
Sbjct: 191 MMRKYGLAADNVIDAYLIDANGKFHDRESMGEDLFWAIRGGAGGSFGIVVAWKVKLVPVP 250

Query: 61  GTVTSCSSNTTLEKDAVKLIHLWQHVAHTLDENLALSIYLSGGI---STQGGKINKNPIV 120
            TVTSCS N TLEKDAVKLIH WQ+VAH LDENL L   LSGG+   S  GG+ NKNPI 
Sbjct: 251 ATVTSCSINRTLEKDAVKLIHRWQYVAHKLDENLFLGTILSGGVLNVSQGGGETNKNPIA 310

Query: 121 LFVTFFLGKADELITILKENFPEWDLAKEDCTESSWIESALINARLIGYPTDESLESLLK 180
           LF T FLGK DEL+ ILK NFPE  LAKEDC ESSWIESAL+NA+L G+P DESLE+LLK
Sbjct: 311 LFFTLFLGKVDELVKILKTNFPELVLAKEDCIESSWIESALVNAKLTGFPADESLETLLK 370

Query: 181 RDTRPLAEASIKIKSGYVKEPIAEAAIKNIWERLKTRDKRREDF 221
           RD  PL +AS+KIKS YVKEPI+E AI+ IWERLKT+DK+ + F
Sbjct: 371 RD--PLTKASMKIKSDYVKEPISETAIEGIWERLKTQDKQGKVF 412

[Close]

BLAST of MC03g_new0287 vs. ExPASy TrEMBL
Match: A0A6J1DHZ2 (berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020223 PE=3 SV=1)

HSP 1 Score: 276 bits (705), Expect = 3.50e-86
Identity = 143/215 (66.51%), Postives = 160/215 (74.42%), Query Frame = 0


Query: 1   MMRKYGLAVDNVIDAYLIDANGKFHDRESMGEDLFWAIRGGGGGSFGSVVVWKVKLVSVP 60
           M+RK+GLA DNVIDAYL+DANGKF DRE MGEDLFWAIRGGGGGSFG VV WKVKLV VP
Sbjct: 196 MLRKFGLAADNVIDAYLVDANGKFQDRELMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVP 255

Query: 61  GTVTSCSSNTTLEKDAVKLIHLWQHVAHTLDENLALSIYLSGGISTQGGKINKNPIVLFV 120
            TVTSC  N TLE+ A+KLIH WQ+VA+ LDENL L I L GG  T  G    NP   F 
Sbjct: 256 ATVTSCYYNRTLEEGAIKLIHRWQYVANKLDENLFLGILLMGGNFTAQGGDKTNPTASFF 315

Query: 121 TFFLGKADELITILKENFPEWDLAKEDCTESSWIESALINARLIGYPTDESLESLLKRDT 180
           + FLGKADE   IL   FPE  LAKE+C ESSWIESAL++A   G+   +SLE+LL R  
Sbjct: 316 SLFLGKADEFFAILNTTFPELGLAKENCLESSWIESALLSA---GFKIGDSLEALLNRT- 375

Query: 181 RPLAEASIKIKSGYVKEPIAEAAIKNIWERLKTRD 215
            PL   SIKIKS YVKEPI+EA I+ IWERLK+RD
Sbjct: 376 -PLTNGSIKIKSDYVKEPISEATIEGIWERLKSRD 405

[Close]

BLAST of MC03g_new0287 vs. ExPASy TrEMBL
Match: A0A6J1CN68 (tetrahydrocannabinolic acid synthase-like OS=Momordica charantia OX=3673 GN=LOC111012666 PE=3 SV=1)

HSP 1 Score: 270 bits (689), Expect = 7.99e-84
Identity = 136/217 (62.67%), Postives = 167/217 (76.96%), Query Frame = 0


Query: 1   MMRKYGLAVDNVIDAYLIDANGKFHDRESMGEDLFWAIRGGGGGSFGSVVVWKVKLVSVP 60
           ++RKYGLA DNVIDAYL+DA+GKFH+RESMGEDLFWAIRGGGGGSFG VV WKVKLV VP
Sbjct: 195 LLRKYGLAADNVIDAYLVDASGKFHNRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVP 254

Query: 61  GTVTSCSSNTTLEKDAVKLIHLWQHVAHTLDENLALSIYLSGG--ISTQGGKINKNPIVL 120
            TVT CS+N TLE+ AVK+ H WQ+VA+ LDENL L I   GG   S +GGK   NP+  
Sbjct: 255 ATVTLCSANRTLEEGAVKITHRWQYVANKLDENLFLGILWLGGKITSQEGGK--TNPVAT 314

Query: 121 FVTFFLGKADELITILKENFPEWDLAKEDCTESSWIESALINARLIGYPTDESLESLLKR 180
           F + FLGKADEL++IL   FPE DLAK+DC E+SWIES ++    +G+PT+ +LE+LL R
Sbjct: 315 FFSLFLGKADELLSILNTKFPELDLAKKDCIETSWIESTVLMG--VGFPTELTLEALLSR 374

Query: 181 DTRPLAEASIKIKSGYVKEPIAEAAIKNIWERLKTRD 215
              PL   S KIKS YVKEP++EA ++ IW+RL ++D
Sbjct: 375 T--PLTNTSSKIKSDYVKEPMSEAVVQGIWKRLDSQD 405

[Close]

BLAST of MC03g_new0287 vs. ExPASy TrEMBL
Match: A0A6J1DF27 (berberine bridge enzyme-like 8 OS=Momordica charantia OX=3673 GN=LOC111020224 PE=3 SV=1)

HSP 1 Score: 268 bits (685), Expect = 3.01e-83
Identity = 139/216 (64.35%), Postives = 161/216 (74.54%), Query Frame = 0


Query: 1   MMRKYGLAVDNVIDAYLIDANGKFHDRESMGEDLFWAIRGGGGGSFGSVVVWKVKLVSVP 60
           M+RKYGLA DNVIDAYLIDANGKF+DRESMGEDLFWAIRGGGGGSFG VV WKVKLV VP
Sbjct: 192 MLRKYGLAADNVIDAYLIDANGKFYDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVP 251

Query: 61  GTVTSCSSNTTLEKDAVKLIHLWQHVAHTLDENLALSIYLSGG-ISTQGGKINKNPIVLF 120
             VT+CSS+  L++D + LIH WQ VA+ LDENL   I L+GG  ST+GG    NP   F
Sbjct: 252 AIVTTCSSDRNLDEDTINLIHRWQSVAYKLDENLYFGIILAGGNASTEGGVKKPNPTATF 311

Query: 121 VTFFLGKADELITILKENFPEWDLAKEDCTESSWIESALINARLIGYPTDESLESLLKRD 180
            + FLGK+DEL+ IL  NFPE +LAK+DC E+SWIES L+ A      T ESLESLL R 
Sbjct: 312 FSLFLGKSDELMAILNTNFPELNLAKKDCVETSWIESTLVAAT--RNQTIESLESLLNRT 371

Query: 181 TRPLAEASIKIKSGYVKEPIAEAAIKNIWERLKTRD 215
             PL   + K+KS YVKEPI +A I+ IWERLK +D
Sbjct: 372 --PLTIENTKLKSDYVKEPIPKATIEGIWERLKAQD 403

[Close]

BLAST of MC03g_new0287 vs. ExPASy TrEMBL
Match: A0A6J1CKW8 (berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111012562 PE=3 SV=1)

HSP 1 Score: 268 bits (684), Expect = 8.56e-83
Identity = 138/216 (63.89%), Postives = 163/216 (75.46%), Query Frame = 0


Query: 1   MMRKYGLAVDNVIDAYLIDANGKFHDRESMGEDLFWAIRGGGGGSFGSVVVWKVKLVSVP 60
           ++RKYGLA DNVIDAYL+DANGKFHDRESMGEDLFWAIRGGGGGSFG VV WKVKLV VP
Sbjct: 196 LLRKYGLAADNVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVP 255

Query: 61  GTVTSCSSNTTLEKDAVKLIHLWQHVAHTLDENLALSI-YLSGGISTQGGKINKNPIVLF 120
            TVT C +N TLE+ AVKL H WQ+VA+ LDENL L I +L G +++QGG    NP+  F
Sbjct: 256 ATVTLCQTNRTLEEGAVKLTHRWQYVANKLDENLFLGILWLGGNVTSQGGG-KTNPVATF 315

Query: 121 VTFFLGKADELITILKENFPEWDLAKEDCTESSWIESALINARLIGYPTDESLESLLKRD 180
            + FLG+ADEL+ IL   FPE DLAK+DC E+SWIES ++    IG+ T  +LE+LL R 
Sbjct: 316 FSLFLGQADELLAILNTKFPELDLAKKDCIETSWIESTVLMG--IGFQTKVTLEALLSRT 375

Query: 181 TRPLAEASIKIKSGYVKEPIAEAAIKNIWERLKTRD 215
             PL   S KIKS YVKEPI+EA I+ I ERL  +D
Sbjct: 376 --PLTNISTKIKSDYVKEPISEATIQGIGERLNAQD 406

[Close]

BLAST of MC03g_new0287 vs. TAIR 10
Match: AT2G34790.1 (FAD-binding Berberine family protein )

HSP 1 Score: 185.7 bits (470), Expect = 4.9e-47
Identity = 103/267 (38.58%), Postives = 159/267 (59.55%), Query Frame = 0


Query: 1   MMRKYGLAVDNVIDAYLIDANGKFHDRESMGEDLFWAIRGGGGGSFGSVVVWKVKLVSVP 60
           MMRK+GL  DNV+DA ++DANG+  DR +MGED+FWAIRGGGGGSFG ++ WK+KLV VP
Sbjct: 196 MMRKFGLGADNVLDARIVDANGQILDRAAMGEDVFWAIRGGGGGSFGVILAWKIKLVPVP 255

Query: 61  GTVTSCSSNTTLEKDAVKLIHLWQHVAHTLDENLALSIYLS-GGISTQGGKINKNPIVLF 120
            TVT  +   TLE+D  K+++ W+ +A  LD++L + + +S    +T+ G  N+   + +
Sbjct: 256 ATVTVFTVTKTLEQDGTKVLYKWEQIADKLDDDLFIRVIISPASKTTKPG--NRTISMSY 315

Query: 121 VTFFLGKADELITILKENFPEWDLAKEDCTESSWIESALINARLIGYPTDESLESLLKRD 180
              FLG ++ L+ +++++FPE  L K+DCTE SWI+S +    + G+P   + E+LL   
Sbjct: 316 QAQFLGDSNRLLQVMQKSFPELGLTKKDCTEMSWIKSVMY---IAGFPNSAAPEALLA-- 375

Query: 181 TRPLAEASIKIKSGYVKEPIAEAAIKNIWERLKTRDKRREDFYTY--TLWRENES---YS 240
            + L +   K KS +VKEPI    ++ +WER    D     +  Y   + R +ES   + 
Sbjct: 376 GKSLFKNHFKAKSDFVKEPIPVEGLEGLWERFLEEDSPLTIWNPYGGMMSRISESEIPFP 435

Query: 241 RVGNSLCAQSWISVQNWLQYPMGRKRH 262
               +L    W+S   W    +  +RH
Sbjct: 436 HRNGTLFKIQWLS--TWQDGKVSEERH 453

[Close]

BLAST of MC03g_new0287 vs. TAIR 10
Match: AT1G30760.1 (FAD-binding Berberine family protein )

HSP 1 Score: 181.4 bits (459), Expect = 9.3e-46
Identity = 93/215 (43.26%), Postives = 136/215 (63.26%), Query Frame = 0


Query: 1   MMRKYGLAVDNVIDAYLIDANGKFHDRESMGEDLFWAIRGGGGGSFGSVVVWKVKLVSVP 60
           MMRK+GL  DNV+DA ++DA+GK  +R +MGED+FWAIRGGGGGSFG ++ WK+KLV VP
Sbjct: 198 MMRKFGLGADNVLDARIVDADGKILNRAAMGEDVFWAIRGGGGGSFGVILAWKIKLVPVP 257

Query: 61  GTVTSCSSNTTLEKDAVKLIHLWQHVAHTLDENLALSIYLSGGISTQGGKINKNPIVLFV 120
             VT  +   TLE+D  KL++ WQ VA  LDE+L + + +     T   K  +     + 
Sbjct: 258 EIVTVFTVTRTLEQDGTKLLYKWQQVADKLDEDLFIRVIIQPTSKTPKSK-ERTISTSYQ 317

Query: 121 TFFLGKADELITILKENFPEWDLAKEDCTESSWIESALINARLIGYPTDESLESLLKRDT 180
             FLG A+ L+ +++ +FP+  L K+DC E+SWI+S +    + G+P+    E+LL  D 
Sbjct: 318 GQFLGDANRLLQVMQRSFPQLGLTKKDCLETSWIKSVMY---IAGFPSTAPSEALL--DG 377

Query: 181 RPLAEASIKIKSGYVKEPIAEAAIKNIWERLKTRD 216
           + L +   K KS YV+EPI    ++ +WE+L   D
Sbjct: 378 KSLFKNYFKAKSDYVEEPIPVEGLEGLWEKLLEED 406

[Close]

BLAST of MC03g_new0287 vs. TAIR 10
Match: AT5G44440.1 (FAD-binding Berberine family protein )

HSP 1 Score: 181.4 bits (459), Expect = 9.3e-46
Identity = 98/218 (44.95%), Postives = 135/218 (61.93%), Query Frame = 0


Query: 1   MMRKYGLAVDNVIDAYLIDANGKFHDRESMGEDLFWAIRGGGGGSFGSVVVWKVKLVSVP 60
           ++RKYGLA DN+IDA ++DA+G+  DR++MGED FWAIRGGGG SFG ++ WKVKLV VP
Sbjct: 191 LLRKYGLAADNIIDALVVDASGRILDRQAMGEDYFWAIRGGGGSSFGVILSWKVKLVDVP 250

Query: 61  GTVTSCSSNTTLEKDAVKLIHLWQHVAHTLDENLALSIYLSGGISTQGGKINKNPI-VLF 120
            T+T      T +K+AV++I  WQ+ A  + ++L +   L         + NKN +  LF
Sbjct: 251 STITVFKVQKTSKKEAVRIIKKWQYAADKVPDDLFIRTTLE--------RSNKNAVHALF 310

Query: 121 VTFFLGKADELITILKENFPEWDLAKEDCTESSWIESALINARLIGYPTDESLESLLKRD 180
              ++G  + L+ +++E FPE  L KE C E SWIES L  A    +P  ESL  L  R+
Sbjct: 311 TGLYIGPVNNLLALMEEKFPELGLEKEGCEEMSWIESVLWFA---DFPKGESLGVLTNRE 370

Query: 181 TRPLAEASIKIKSGYVKEPIAEAAIKNIWERLKTRDKR 218
              L   S K K  +V+EPI EAAI+ IW RL+  + R
Sbjct: 371 RTSL---SFKGKDDFVQEPIPEAAIQEIWRRLEAPEAR 394

[Close]

BLAST of MC03g_new0287 vs. TAIR 10
Match: AT5G44380.1 (FAD-binding Berberine family protein )

HSP 1 Score: 179.1 bits (453), Expect = 4.6e-45
Identity = 97/212 (45.75%), Postives = 135/212 (63.68%), Query Frame = 0


Query: 1   MMRKYGLAVDNVIDAYLIDANGKFHDRESMGEDLFWAIRGGGGGSFGSVVVWKVKLVSVP 60
           +MRKYGLA DNV+D  ++DANGK  DR +MGEDLFWAIRGGGG SFG V+ WK+KLV VP
Sbjct: 201 LMRKYGLAGDNVLDVKMVDANGKLLDRAAMGEDLFWAIRGGGGASFGIVLAWKIKLVPVP 260

Query: 61  GTVTSCSSNTTLEKDA-VKLIHLWQHVAHTLDENLALSIYLSGGISTQGGKINKNPIVLF 120
            TVT  +   TLE+DA +K I  WQ ++  + E + + + L       G   NK   + +
Sbjct: 261 KTVTVFTVTKTLEQDARLKTISKWQQISSKIIEEIHIRVVLRAA----GNDGNKTVTMTY 320

Query: 121 VTFFLGKADELITILKENFPEWDLAKEDCTESSWIESALINARLIGYPTDESLESLLKRD 180
           +  FLG+   L+ ++++ FPE  L ++DCTE SWIE+AL +    G+PT   +E LL+  
Sbjct: 321 LGQFLGEKGTLLKVMEKAFPELGLTQKDCTEMSWIEAALFHG---GFPTGSPIEILLQLK 380

Query: 181 TRPLAEASIKIKSGYVKEPIAEAAIKNIWERL 212
           + PL +   K  S +VKEPI    +K I++RL
Sbjct: 381 S-PLGKDYFKATSDFVKEPIPVIGLKGIFKRL 404

[Close]

BLAST of MC03g_new0287 vs. TAIR 10
Match: AT5G44390.1 (FAD-binding Berberine family protein )

HSP 1 Score: 175.3 bits (443), Expect = 6.6e-44
Identity = 92/218 (42.20%), Postives = 139/218 (63.76%), Query Frame = 0


Query: 1   MMRKYGLAVDNVIDAYLIDANGKFHDRESMGEDLFWAIRGGGGGSFGSVVVWKVKLVSVP 60
           +MRKYGLA DNV+DA ++DANGK  DR SMGEDLFWAIRGG GGSFG ++ WK+KLV VP
Sbjct: 200 LMRKYGLAADNVLDAKIVDANGKLLDRASMGEDLFWAIRGGSGGSFGIILSWKIKLVPVP 259

Query: 61  GTVTSCSSNTTLEKD-AVKLIHLWQHVAHTLDENLALSIYLSGGISTQGGKINKNPIVLF 120
            T+T  +   T E+D + K++  WQ +A  L + L L ++     +  G K NK   + +
Sbjct: 260 ETLTVFTVTKTFEQDRSFKILSKWQEIADNLVDELFLRVF----FTVSGNKANKTVTMAY 319

Query: 121 VTFFLGKADELITILKENFPEWDLAKEDCTESSWIESALINARLIGYPTD--ESLESLLK 180
           +  FLG+   L+ ++K++FPE  L ++DC E SWI+S + N+   G+PT+    +E LL+
Sbjct: 320 IGQFLGEKGTLMEVMKKDFPELGLTQKDCIEMSWIDSIIYNS---GFPTNPPPPIEILLQ 379

Query: 181 RDTRPLAEASIKIKSGYVKEPIAEAAIKNIWERLKTRD 216
             + P+ +   K KS + K+PI    ++ ++++L   D
Sbjct: 380 AKS-PIGKVYFKGKSDFAKKPIPVLGLEGMFKKLLEED 409

The following BLAST results are available for this feature:

BLAST of MC03g_new0287 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A2S1XB67	3.1e-46	44.98	O-acetylstemmadenine oxidase OS=Catharanthus roseus OX=4058 GN=ASO PE=1 SV=1	[more]
O64743	6.9e-46	38.58	Berberine bridge enzyme-like 15 OS=Arabidopsis thaliana OX=3702 GN=MEE23 PE=1 SV...	[more]
Q93ZA3	1.3e-44	43.26	Berberine bridge enzyme-like 13 OS=Arabidopsis thaliana OX=3702 GN=At1g30760 PE=...	[more]
Q9FI21	1.3e-44	44.95	Berberine bridge enzyme-like 28 OS=Arabidopsis thaliana OX=3702 GN=At5g44440 PE=...	[more]
Q9FKV0	6.5e-44	45.75	Berberine bridge enzyme-like 24 OS=Arabidopsis thaliana OX=3702 GN=At5g44380 PE=...	[more]

BLAST of MC03g_new0287 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022152424.1	1.34e-109	75.89	berberine bridge enzyme-like 28 [Momordica charantia]	[more]
XP_038895343.1	6.82e-87	67.13	cannabidiolic acid synthase-like [Benincasa hispida]	[more]
XP_022152516.1	7.22e-86	66.51	berberine bridge enzyme-like 18 [Momordica charantia]	[more]
XP_022142587.1	1.65e-83	62.67	tetrahydrocannabinolic acid synthase-like [Momordica charantia]	[more]
XP_022152518.1	6.21e-83	64.35	berberine bridge enzyme-like 8 [Momordica charantia]	[more]

BLAST of MC03g_new0287 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DFZ4	6.49e-110	75.89	berberine bridge enzyme-like 28 OS=Momordica charantia OX=3673 GN=LOC111020160 P...	[more]
A0A6J1DHZ2	3.50e-86	66.51	berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020223 P...	[more]
A0A6J1CN68	7.99e-84	62.67	tetrahydrocannabinolic acid synthase-like OS=Momordica charantia OX=3673 GN=LOC1...	[more]
A0A6J1DF27	3.01e-83	64.35	berberine bridge enzyme-like 8 OS=Momordica charantia OX=3673 GN=LOC111020224 PE...	[more]
A0A6J1CKW8	8.56e-83	63.89	berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111012562 P...	[more]

BLAST of MC03g_new0287 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G34790.1	4.9e-47	38.58	FAD-binding Berberine family protein	[more]
AT1G30760.1	9.3e-46	43.26	FAD-binding Berberine family protein	[more]
AT5G44440.1	9.3e-46	44.95	FAD-binding Berberine family protein	[more]
AT5G44380.1	4.6e-45	45.75	FAD-binding Berberine family protein	[more]
AT5G44390.1	6.6e-44	42.20	FAD-binding Berberine family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.462.20		coord: 62..219 e-value: 7.8E-62 score: 211.8
None	No IPR available	PANTHER	PTHR32448	OS08G0158400 PROTEIN	coord: 1..215
IPR016169	FAD-binding, type PCMH, subdomain 2	GENE3D	3.30.465.10		coord: 1..181 e-value: 7.8E-62 score: 211.8
IPR036318	FAD-binding, type PCMH-like superfamily	SUPERFAMILY	56176	FAD-binding/transporter-associated domain-like	coord: 4..60

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC03g_new0287.1	MC03g_new0287.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0071949	FAD binding
molecular_function	GO:0016491	oxidoreductase activity
molecular_function	GO:0050660	flavin adenine dinucleotide binding