MC03g_new0285 (gene) Bitter gourd (Dali-11) v1

BLAST of MC03g_new0285 vs. ExPASy Swiss-Prot
Match: Q8GTB6 (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 GN=THCAS PE=1 SV=1)

HSP 1 Score: 527.7 bits (1358), Expect = 1.5e-148
Identity = 251/515 (48.74%), Postives = 351/515 (68.16%), Query Frame = 0

Query: 27  AEKHEAFLQCLSHHSADSSSISKVIYTPINPSYSSVLNFSVRNLRFSSAETPKPLLIITP 86
           A   E FL+C S H  ++ +  K++YT  +  Y S+LN +++NLRF S  TPKPL+I+TP
Sbjct: 28  ANPRENFLKCFSKHIPNNVANPKLVYTQHDQLYMSILNSTIQNLRFISDTTPKPLVIVTP 87

Query: 87  SYVSHVQAAVICSKTHGLQIRIRSGGHDIEGLSYVAHLPFIIIDLINLKSITVNIENSTA 146
           S  SH+QA ++CSK  GLQIR RSGGHD EG+SY++ +PF+++DL N+ SI +++ + TA
Sbjct: 88  SNNSHIQATILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVVVDLRNMHSIKIDVHSQTA 147

Query: 147 WIQSGATLGELYYNIAQKSRTLAFPAGVCPTVGVGGHFSGGGYGLMLRKYGLAADNVIDA 206
           W+++GATLGE+YY I +K+  L+FP G CPTVGVGGHFSGGGYG ++R YGLAADN+IDA
Sbjct: 148 WVEAGATLGEVYYWINEKNENLSFPGGYCPTVGVGGHFSGGGYGALMRNYGLAADNIIDA 207

Query: 207 YLIDANGKFYDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPAIVTTCSSDRNLD-E 266
           +L++ +GK  DR+SMGEDLFWAIRGGGG +FGI+ AWK+KLV VP+  T  S  +N++  
Sbjct: 208 HLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVAVPSKSTIFSVKKNMEIH 267

Query: 267 DTINLIHRWQSVAYKLDENLYFGIILAGGNASTEGGVKKPNPTATFFSLFLGKSDELMAI 326
             + L ++WQ++AYK D++L         N +   G  K      F S+F G  D L+ +
Sbjct: 268 GLVKLFNKWQNIAYKYDKDLVLMTHFITKNITDNHGKNKTTVHGYFSSIFHGGVDSLVDL 327

Query: 327 LNTNFPELNLAKKDCVETSWIESTLV---AATRNQTIESLESLLNRTPLTIENTKLKSDY 386
           +N +FPEL + K DC E SWI++T+        N      E LL+R+        +K DY
Sbjct: 328 MNKSFPELGIKKTDCKEFSWIDTTIFYSGVVNFNTANFKKEILLDRSAGKKTAFSIKLDY 387

Query: 387 VKEPIPKATIEGIWERLKAQDIEVPQLVFVPYGGRMSRISDSETPFSHRARNLYKIGYIV 446
           VK+PIP+  +  I E+L  +D+     V  PYGG M  IS+S  PF HRA  +Y++ Y  
Sbjct: 388 VKKPIPETAMVKILEKLYEEDVGAGMYVLYPYGGIMEEISESAIPFPHRAGIMYELWYTA 447

Query: 447 GWKEQSVSAEERHLSWIREIYDYMTPFVSKSPRATYVNYRDLDIGTNNKYGKTSYKQASV 506
            W++Q     E+H++W+R +Y++ TP+VS++PR  Y+NYRDLD+G  N     +Y QA +
Sbjct: 448 SWEKQ--EDNEKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLGKTNHASPNNYTQARI 507

Query: 507 WGSKYFGNNFNRLVHIKSKVDPSNFFKNEQSIPPL 538
           WG KYFG NFNRLV +K+KVDP+NFF+NEQSIPPL
Sbjct: 508 WGEKYFGKNFNRLVKVKTKVDPNNFFRNEQSIPPL 540

BLAST of MC03g_new0285 vs. ExPASy Swiss-Prot
Match: Q9SA85 (Berberine bridge enzyme-like 8 OS=Arabidopsis thaliana OX=3702 GN=At1g30700 PE=2 SV=1)

HSP 1 Score: 525.4 bits (1352), Expect = 7.6e-148
Identity = 267/542 (49.26%), Postives = 368/542 (67.90%), Query Frame = 0

Query: 1   MKYSPLTILSLAFILLSSSSSWAAASAEKHEAFLQCLSHHSADSSSISKVIYTPINPSYS 60
           MKY+ + +L     +  SSSS A       E F QCL+ +S     IS  I+   N SYS
Sbjct: 1   MKYALILVLFFVVFIWQSSSSSA-----NSETFTQCLTSNSDPKHPISPAIFFSGNGSYS 60

Query: 61  SVLNFSVRNLRFSSAETPKPLLIITPSYVSHVQAAVICSKTHGLQIRIRSGGHDIEGLSY 120
           SVL  ++RNLRF++  TPKP LII  ++ SHVQAA+ C K H LQ++IRSGGHD +GLSY
Sbjct: 61  SVLQANIRNLRFNTTSTPKPFLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGLSY 120

Query: 121 VAH--LPFIIIDLINLKSITVNIENSTAWIQSGATLGELYYNIAQKSRTLAFPAGVCPTV 180
           V +   PF ++D+ NL+S+ V++ + TAW+Q+GA LGE+YY I +KS+TLA+PAG+CPTV
Sbjct: 121 VTYSGKPFFVLDMFNLRSVDVDVASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGICPTV 180

Query: 181 GVGGHFSGGGYGLMLRKYGLAADNVIDAYLIDANGKFYDRESMGEDLFWAIRGGGGGSFG 240
           GVGGH SGGGYG M+RKYGL  DN IDA ++D NGK  DR+ MGEDL+WAI GGGGGS+G
Sbjct: 181 GVGGHISGGGYGNMMRKYGLTVDNTIDARMVDVNGKILDRKLMGEDLYWAINGGGGGSYG 240

Query: 241 IVVAWKVKLVPVPAIVTTCSSDRNLDEDTINLIHRWQSVAYKLDENLYFGIIL--AGGNA 300
           +V+A+K+ LV VP  VT     R L+++  ++IHRWQ VA KL + L+   ++    G  
Sbjct: 241 VVLAYKINLVEVPENVTVFRISRTLEQNATDIIHRWQQVAPKLPDELFIRTVIDVVNGTV 300

Query: 301 STEGGVKKPNPTATFFSLFLGKSDELMAILNTNFPELNLAKKDCVETSWIESTLVAATRN 360
           S++  V+      TF ++FLG +  L++ILN  FPEL L + DC ETSWI+S L     N
Sbjct: 301 SSQKTVR-----TTFIAMFLGDTTTLLSILNRRFPELGLVRSDCTETSWIQSVLF--WTN 360

Query: 361 QTIESLESLLNRTPLTIENTKLKSDYVKEPIPKATIEGIWERLKAQDIEVPQLVFVPYGG 420
             + S E+LL +    +   K KSDYV+EPI +  +E IW+  K  ++E+P + F PYGG
Sbjct: 361 IQVGSSETLLLQRNQPVNYLKRKSDYVREPISRTGLESIWK--KMIELEIPTMAFNPYGG 420

Query: 421 RMSRISDSETPFSHRARNLYKIGYIVGWKEQSVSAEERHLSWIREIYDYMTPFVSKSPRA 480
            M RIS + TPF +RA NL+KI Y   W++++++  +R++   R++Y +MTPFVSK+PR 
Sbjct: 421 EMGRISSTVTPFPYRAGNLWKIQYGANWRDETLT--DRYMELTRKLYQFMTPFVSKNPRQ 480

Query: 481 TYVNYRDLDIGTNNKYGK-TSYKQASVWGSKYFGNNFNRLVHIKSKVDPSNFFKNEQSIP 538
           ++ NYRD+D+G N+  GK +SY +   +G KYF  NF RLV IK++VD  NFF+NEQSIP
Sbjct: 481 SFFNYRDVDLGINSHNGKISSYVEGKRYGKKYFAGNFERLVKIKTRVDSGNFFRNEQSIP 526

BLAST of MC03g_new0285 vs. ExPASy Swiss-Prot
Match: A6P6W0 (Cannabidiolic acid synthase-like 1 OS=Cannabis sativa OX=3483 GN=CBDAS2 PE=2 SV=1)

HSP 1 Score: 525.4 bits (1352), Expect = 7.6e-148
Identity = 253/534 (47.38%), Postives = 363/534 (67.98%), Query Frame = 0

Query: 8   ILSLAFILLSSSSSWAAASAEKHEAFLQCLSHHSADSSSISKVIYTPINPSYSSVLNFSV 67
           +  + F  LS +   + A+ +  E FL+C S +   + + +K++YT  +  Y S+LN ++
Sbjct: 11  VCKIIFFFLSFNIQISIANPQ--ENFLKCFSQYIPTNVTNAKLVYTQHDQFYMSILNSTI 70

Query: 68  RNLRFSSAETPKPLLIITPSYVSHVQAAVICSKTHGLQIRIRSGGHDIEGLSYVAHLPFI 127
           +NLRF+S  TPKPL+IITP  VSH+Q  ++CSK  GLQIR RSGGHD EG+SY++ +PF+
Sbjct: 71  QNLRFTSDTTPKPLVIITPLNVSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFV 130

Query: 128 IIDLINLKSITVNIENSTAWIQSGATLGELYYNIAQKSRTLAFPAGVCPTVGVGGHFSGG 187
           I+DL N+ S+ +++ + TAW+++GATLGE+YY I + +  L+FPAG CPTVG GGHFSGG
Sbjct: 131 IVDLRNMHSVKIDVHSQTAWVEAGATLGEVYYWINENNENLSFPAGYCPTVGAGGHFSGG 190

Query: 188 GYGLMLRKYGLAADNVIDAYLIDANGKFYDRESMGEDLFWAIRGGGGGSFGIVVAWKVKL 247
           GYG ++R YGLAADN+IDA+L++ +GK  DR+SMGEDLFWAIRGGGG +FGI+ AWK++L
Sbjct: 191 GYGALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRL 250

Query: 248 VPVPAIVTTCSSDRNLD-EDTINLIHRWQSVAYKLDENLYFGIILAGGNASTEGGVKKPN 307
           V VP++ T  S  +N++  + + L+++WQ++AY  ++ L         N +   G  K  
Sbjct: 251 VAVPSMSTIFSVKKNMEIHELVKLVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTT 310

Query: 308 PTATFFSLFLGKSDELMAILNTNFPELNLAKKDCVETSWIESTLV---AATRNQTIESLE 367
             + F S+F G  D L+ ++N +FPEL + K DC + SWI++ +        N T    E
Sbjct: 311 IHSYFSSIFHGGVDSLVDLMNKSFPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTYFKKE 370

Query: 368 SLLNRTPLTIENTKLKSDYVKEPIPKATIEGIWERLKAQDIEVPQLVFVPYGGRMSRISD 427
            LL+R+        +K DYVK+PIP+  +  I E+L  +D+ V   VF PYGG M  IS+
Sbjct: 371 ILLDRSGGRKAAFSIKLDYVKKPIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISE 430

Query: 428 SETPFSHRARNLYKIGYIVGWKEQSVSAEERHLSWIREIYDYMTPFVSKSPRATYVNYRD 487
           S  PF HRA  +Y+I YI  W++Q     E+H++WIR +Y++ TP+VS++PR  Y+NYRD
Sbjct: 431 SAIPFPHRAGIMYEIWYIASWEKQ--EDNEKHINWIRNVYNFTTPYVSQNPRMAYLNYRD 490

Query: 488 LDIGTNNKYGKTSYKQASVWGSKYFGNNFNRLVHIKSKVDPSNFFKNEQSIPPL 538
           LD+G  N     +Y QA +WG KYFG NFNRLV +K+KVDP NFF+NEQSIPPL
Sbjct: 491 LDLGKTNFESPNNYTQARIWGEKYFGKNFNRLVKVKTKVDPDNFFRNEQSIPPL 540

BLAST of MC03g_new0285 vs. ExPASy Swiss-Prot
Match: A6P6W1 (Cannabidiolic acid synthase-like 2 OS=Cannabis sativa OX=3483 GN=CBDAS3 PE=2 SV=1)

HSP 1 Score: 524.6 bits (1350), Expect = 1.3e-147
Identity = 255/534 (47.75%), Postives = 362/534 (67.79%), Query Frame = 0

Query: 8   ILSLAFILLSSSSSWAAASAEKHEAFLQCLSHHSADSSSISKVIYTPINPSYSSVLNFSV 67
           +  + F  LS +   + A+ +  E FL+CLS +   + + +K++YT  +  Y S+LN +V
Sbjct: 11  VCKIIFFFLSFNIQISIANPQ--ENFLKCLSQYIPTNVTNAKLVYTQHDQFYMSILNSTV 70

Query: 68  RNLRFSSAETPKPLLIITPSYVSHVQAAVICSKTHGLQIRIRSGGHDIEGLSYVAHLPFI 127
           +NLRF+S  TPKPL+I TP  VSH+Q  ++CSK  GLQIR RSGGHD EG+SY++ +PF+
Sbjct: 71  QNLRFTSDTTPKPLVITTPLNVSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFV 130

Query: 128 IIDLINLKSITVNIENSTAWIQSGATLGELYYNIAQKSRTLAFPAGVCPTVGVGGHFSGG 187
           I+DL N+ S+ +++ + TAW++SGATLGE+YY I + +  L+FPAG CPTVG GGHFSGG
Sbjct: 131 IVDLRNMHSVKIDVHSQTAWVESGATLGEVYYWINENNENLSFPAGYCPTVGTGGHFSGG 190

Query: 188 GYGLMLRKYGLAADNVIDAYLIDANGKFYDRESMGEDLFWAIRGGGGGSFGIVVAWKVKL 247
           GYG ++R YGLAADN+IDA+L++ +GK  DR+SMGEDLFWAIRGGGG +FGI+ AWK++L
Sbjct: 191 GYGALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRL 250

Query: 248 VPVPAIVTTCSSDRNLD-EDTINLIHRWQSVAYKLDENLYFGIILAGGNASTEGGVKKPN 307
           V VP++ T  S  +N++  + + L+++WQ++AY  ++ L         N +   G  K  
Sbjct: 251 VAVPSMSTIFSVKKNMEIHELVKLVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTT 310

Query: 308 PTATFFSLFLGKSDELMAILNTNFPELNLAKKDCVETSWIESTLV---AATRNQTIESLE 367
             + F S+F G  D L+ ++N +FPEL + K DC + SWI++ +        N T    E
Sbjct: 311 IHSYFSSIFHGGVDSLVDLMNKSFPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTNFKKE 370

Query: 368 SLLNRTPLTIENTKLKSDYVKEPIPKATIEGIWERLKAQDIEVPQLVFVPYGGRMSRISD 427
            LL+R+        +K DYVK+PIP+  +  I E+L  +D+ V   VF PYGG M  IS+
Sbjct: 371 ILLDRSGGRKAAFSIKLDYVKKPIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISE 430

Query: 428 SETPFSHRARNLYKIGYIVGWKEQSVSAEERHLSWIREIYDYMTPFVSKSPRATYVNYRD 487
           S  PF HRA   Y+I YI  W++Q     E+H++WIR +Y++ TP+VS++PR  Y+NYRD
Sbjct: 431 SAIPFPHRAGITYEIWYIASWEKQ--EDNEKHINWIRNVYNFTTPYVSQNPRMAYLNYRD 490

Query: 488 LDIGTNNKYGKTSYKQASVWGSKYFGNNFNRLVHIKSKVDPSNFFKNEQSIPPL 538
           LD+G  N     +Y QA +WG KYFG NFNRLV +K+KVDP NFF+NEQSIPPL
Sbjct: 491 LDLGKTNFESPNNYTQARIWGEKYFGKNFNRLVKVKTKVDPDNFFRNEQSIPPL 540

BLAST of MC03g_new0285 vs. ExPASy Swiss-Prot
Match: Q9SVG5 (Berberine bridge enzyme-like 18 OS=Arabidopsis thaliana OX=3702 GN=At4g20820 PE=3 SV=1)

HSP 1 Score: 521.5 bits (1342), Expect = 1.1e-146
Identity = 261/534 (48.88%), Postives = 361/534 (67.60%), Query Frame = 0

Query: 3   YSPLTILSLAFILLSSSSSWAAASAEKHEAFLQCLSHHSADSSSISKVIYTPINPSYSSV 62
           +S + I      LL S     +A+     +FLQCLS    DS+ +SKVI+TP + S+SSV
Sbjct: 7   FSSVLIFFTTSTLLLSIPHPVSANRSNQSSFLQCLSLQLNDSNIVSKVIHTPNDTSFSSV 66

Query: 63  LNFSVRNLRFSSAETPKPLLIITPSYVSHVQAAVICSKTHGLQIRIRSGGHDIEGLSYVA 122
           L  S++N RFS+ + PKP+LI+TP   S VQ+AV C++  G+ IR RSGGHD EGLSYV 
Sbjct: 67  LASSIQNQRFSAPDVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSYVT 126

Query: 123 HLPFIIIDLINLKSITVNIENSTAWIQSGATLGELYYNIAQKSRTLAFPAGVCPTVGVGG 182
           H PF+I+DL NL+SITV+++N + W+Q+GAT+GELYY I +K+RTLAFPAGVCPTVGVGG
Sbjct: 127 HKPFVILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGVGG 186

Query: 183 HFSGGGYGLMLRKYGLAADNVIDAYLIDANGKFYDRESMGEDLFWAIRGGGGGSFGIVVA 242
           HFSGGGYG +LRK+GLAAD+VIDA ++DA G+  +R  MGED FWAIRGGGG SF +V++
Sbjct: 187 HFSGGGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGGGSSFCVVLS 246

Query: 243 WKVKLVPVPAIVTTCSSDRNLDEDTINLIHRWQSVAYKLDENLYFGIILAGGNASTEGGV 302
           WK+ L+ VP+ VT  +  +  ++  + +IHRWQ VA K+ ++L+  ++L           
Sbjct: 247 WKIGLINVPSTVTVFNVTKFSEQSALKIIHRWQFVADKVSDDLFIRVMLQR--------- 306

Query: 303 KKPNPTATFFSLFLGKSDELMAILNTNFPELNLAKKDCVETSWIESTLVAATRNQTIESL 362
            K    A+F  L+LG    L+ ++N  FPEL L + DC E SWIES +  A   +  E +
Sbjct: 307 YKNMVRASFPGLYLGSVKNLLKMVNKEFPELGLEEDDCTEMSWIESVIWFAELGE--EPI 366

Query: 363 ESLLNRTPLTIENTKLKSDYVKEPIPKATIEGIWERLKAQDIEVPQLVFVPYGGRMSRIS 422
             L  RT  ++   K KSD+V+EP+PK  I  +W RL+  + E  QL+F P+GG+MS I+
Sbjct: 367 NVLTKRTRASLA-FKAKSDFVQEPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGKMSEIA 426

Query: 423 DSETPFSHRARNLYKIGYIVGWKEQSVSAEERHLSWIREIYDYMTPFVSKSPRATYVNYR 482
           D ETPF HR  N+Y+I Y+  W+      +E+++ W+  +YD M+ FV+KSPR  Y+N R
Sbjct: 427 DYETPFPHRKGNIYEIQYLNYWRG---DVKEKYMRWVERVYDDMSEFVAKSPRGAYINLR 486

Query: 483 DLDIGTNNKYGKTSYKQASVWGSKYFGNNFNRLVHIKSKVDPSNFFKNEQSIPP 537
           DLD+G      ++ Y++   WG KYF NNF RLV +K+ VDPS+FF +EQSIPP
Sbjct: 487 DLDLGMYVGVKRSKYEEGKSWGVKYFKNNFERLVRVKTSVDPSDFFCDEQSIPP 525

BLAST of MC03g_new0285 vs. NCBI nr
Match: XP_022152518.1 (berberine bridge enzyme-like 8 [Momordica charantia])

HSP 1 Score: 1068 bits (2762), Expect = 0.0
Identity = 537/537 (100.00%), Postives = 537/537 (100.00%), Query Frame = 0

Query: 1   MKYSPLTILSLAFILLSSSSSWAAASAEKHEAFLQCLSHHSADSSSISKVIYTPINPSYS 60
           MKYSPLTILSLAFILLSSSSSWAAASAEKHEAFLQCLSHHSADSSSISKVIYTPINPSYS
Sbjct: 1   MKYSPLTILSLAFILLSSSSSWAAASAEKHEAFLQCLSHHSADSSSISKVIYTPINPSYS 60

Query: 61  SVLNFSVRNLRFSSAETPKPLLIITPSYVSHVQAAVICSKTHGLQIRIRSGGHDIEGLSY 120
           SVLNFSVRNLRFSSAETPKPLLIITPSYVSHVQAAVICSKTHGLQIRIRSGGHDIEGLSY
Sbjct: 61  SVLNFSVRNLRFSSAETPKPLLIITPSYVSHVQAAVICSKTHGLQIRIRSGGHDIEGLSY 120

Query: 121 VAHLPFIIIDLINLKSITVNIENSTAWIQSGATLGELYYNIAQKSRTLAFPAGVCPTVGV 180
           VAHLPFIIIDLINLKSITVNIENSTAWIQSGATLGELYYNIAQKSRTLAFPAGVCPTVGV
Sbjct: 121 VAHLPFIIIDLINLKSITVNIENSTAWIQSGATLGELYYNIAQKSRTLAFPAGVCPTVGV 180

Query: 181 GGHFSGGGYGLMLRKYGLAADNVIDAYLIDANGKFYDRESMGEDLFWAIRGGGGGSFGIV 240
           GGHFSGGGYGLMLRKYGLAADNVIDAYLIDANGKFYDRESMGEDLFWAIRGGGGGSFGIV
Sbjct: 181 GGHFSGGGYGLMLRKYGLAADNVIDAYLIDANGKFYDRESMGEDLFWAIRGGGGGSFGIV 240

Query: 241 VAWKVKLVPVPAIVTTCSSDRNLDEDTINLIHRWQSVAYKLDENLYFGIILAGGNASTEG 300
           VAWKVKLVPVPAIVTTCSSDRNLDEDTINLIHRWQSVAYKLDENLYFGIILAGGNASTEG
Sbjct: 241 VAWKVKLVPVPAIVTTCSSDRNLDEDTINLIHRWQSVAYKLDENLYFGIILAGGNASTEG 300

Query: 301 GVKKPNPTATFFSLFLGKSDELMAILNTNFPELNLAKKDCVETSWIESTLVAATRNQTIE 360
           GVKKPNPTATFFSLFLGKSDELMAILNTNFPELNLAKKDCVETSWIESTLVAATRNQTIE
Sbjct: 301 GVKKPNPTATFFSLFLGKSDELMAILNTNFPELNLAKKDCVETSWIESTLVAATRNQTIE 360

Query: 361 SLESLLNRTPLTIENTKLKSDYVKEPIPKATIEGIWERLKAQDIEVPQLVFVPYGGRMSR 420
           SLESLLNRTPLTIENTKLKSDYVKEPIPKATIEGIWERLKAQDIEVPQLVFVPYGGRMSR
Sbjct: 361 SLESLLNRTPLTIENTKLKSDYVKEPIPKATIEGIWERLKAQDIEVPQLVFVPYGGRMSR 420

Query: 421 ISDSETPFSHRARNLYKIGYIVGWKEQSVSAEERHLSWIREIYDYMTPFVSKSPRATYVN 480
           ISDSETPFSHRARNLYKIGYIVGWKEQSVSAEERHLSWIREIYDYMTPFVSKSPRATYVN
Sbjct: 421 ISDSETPFSHRARNLYKIGYIVGWKEQSVSAEERHLSWIREIYDYMTPFVSKSPRATYVN 480

Query: 481 YRDLDIGTNNKYGKTSYKQASVWGSKYFGNNFNRLVHIKSKVDPSNFFKNEQSIPPL 537
           YRDLDIGTNNKYGKTSYKQASVWGSKYFGNNFNRLVHIKSKVDPSNFFKNEQSIPPL
Sbjct: 481 YRDLDIGTNNKYGKTSYKQASVWGSKYFGNNFNRLVHIKSKVDPSNFFKNEQSIPPL 537

BLAST of MC03g_new0285 vs. NCBI nr
Match: XP_022152519.1 (tetrahydrocannabinolic acid synthase-like [Momordica charantia])

HSP 1 Score: 882 bits (2279), Expect = 0.0
Identity = 435/537 (81.01%), Postives = 480/537 (89.39%), Query Frame = 0

Query: 1   MKYSPLTILSLAFILLSSSSSWAAASAEKHEAFLQCLSHHSADSSSISKVIYTPINPSYS 60
           MKYSPL ILSLAFILLSSSSSWA A AEKHE+FLQCL HHS++SSSISKVIY+PIN SYS
Sbjct: 1   MKYSPLIILSLAFILLSSSSSWAVACAEKHESFLQCLFHHSSNSSSISKVIYSPINSSYS 60

Query: 61  SVLNFSVRNLRFSSAETPKPLLIITPSYVSHVQAAVICSKTHGLQIRIRSGGHDIEGLSY 120
           SVLN SV+N RF +A+TPKPL IITPS++SH+Q AVICSKTHG QIR RS GHD EGLSY
Sbjct: 61  SVLNSSVQNFRFLTAKTPKPLFIITPSHLSHIQIAVICSKTHGFQIRTRSDGHDTEGLSY 120

Query: 121 VAHLPFIIIDLINLKSITVNIENSTAWIQSGATLGELYYNIAQKSRTLAFPAGVCPTVGV 180
           VA++PFIIIDLINL+S+T+N+E+STAW+QSGATLGELYY+IAQKS+TLAFPAGVCPTVGV
Sbjct: 121 VAYVPFIIIDLINLRSVTINVEHSTAWVQSGATLGELYYSIAQKSQTLAFPAGVCPTVGV 180

Query: 181 GGHFSGGGYGLMLRKYGLAADNVIDAYLIDANGKFYDRESMGEDLFWAIRGGGGGSFGIV 240
           GGH SGGGYGLMLRKYG AADNVIDAYLIDANGKF+DRESMGEDLFWAIRGGGGGS GIV
Sbjct: 181 GGHSSGGGYGLMLRKYGFAADNVIDAYLIDANGKFHDRESMGEDLFWAIRGGGGGSLGIV 240

Query: 241 VAWKVKLVPVPAIVTTCSSDRNLDEDTINLIHRWQSVAYKLDENLYFGIILAGGNASTEG 300
           VAWKVKLVPVPA VT C ++RNLDED + LIHRWQ VA KLDENLY GI+L GGNAST+G
Sbjct: 241 VAWKVKLVPVPATVTICLTNRNLDEDLVKLIHRWQFVAPKLDENLYVGIMLNGGNASTQG 300

Query: 301 GVKKPNPTATFFSLFLGKSDELMAILNTNFPELNLAKKDCVETSWIESTLVAATRNQTIE 360
           GV   NP +TFFSLFLGK DELMAIL  +FPE+NLA  DCVETSWIEST++AATRN+T+E
Sbjct: 301 GV---NPKSTFFSLFLGKIDELMAILKRDFPEMNLANNDCVETSWIESTIIAATRNKTVE 360

Query: 361 SLESLLNRTPLTIENTKLKSDYVKEPIPKATIEGIWERLKAQDIEVPQLVFVPYGGRMSR 420
           SLE LL + PL   + K KSDYVKEPIPK  IEGIWERLKAQDIE  QLV VPYGGRMS+
Sbjct: 361 SLEYLLTKIPLATGSIKTKSDYVKEPIPKVAIEGIWERLKAQDIEASQLVIVPYGGRMSQ 420

Query: 421 ISDSETPFSHRARNLYKIGYIVGWKEQSVSAEERHLSWIREIYDYMTPFVSKSPRATYVN 480
           I DSE P+SHRA  LYKIGY+VGWKEQSV   +RHLSWIRE+YDYMTPFVSKSPRA YVN
Sbjct: 421 ILDSEIPYSHRAGYLYKIGYVVGWKEQSVDVGKRHLSWIRELYDYMTPFVSKSPRAAYVN 480

Query: 481 YRDLDIGTNNKYGKTSYKQASVWGSKYFGNNFNRLVHIKSKVDPSNFFKNEQSIPPL 537
           YRDLDIGTNNKYGKTSYK+AS+WGSKYFG NFNRLVH+KSKVDPSNFF++EQSIPPL
Sbjct: 481 YRDLDIGTNNKYGKTSYKRASIWGSKYFGKNFNRLVHVKSKVDPSNFFRHEQSIPPL 534

BLAST of MC03g_new0285 vs. NCBI nr
Match: XP_038895508.1 (berberine bridge enzyme-like 28 [Benincasa hispida])

HSP 1 Score: 838 bits (2166), Expect = 9.61e-303
Identity = 411/539 (76.25%), Postives = 479/539 (88.87%), Query Frame = 0

Query: 1   MKY-SPLTILSLAFILLSSSSSWAAASAEKHEAFLQCLSHHSADSSSISKVIYTPINPSY 60
           MKY SPL +LSL  I++SSS  WA+AS +K++ FLQCL  HS D+  ISKV+Y+PIN SY
Sbjct: 1   MKYTSPLILLSLVLIVVSSSPLWASASEQKYQHFLQCLLEHSLDNYPISKVVYSPINSSY 60

Query: 61  SSVLNFSVRNLRFSSAETPKPLLIITPSYVSHVQAAVICSKTHGLQIRIRSGGHDIEGLS 120
           SSVL+FS+RNLRFS  +TPKPLLIITPS+VSH+QAAVICSK+HGLQIR RSGGHD EGLS
Sbjct: 61  SSVLDFSIRNLRFSKPQTPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGLS 120

Query: 121 YVAHLPFIIIDLINLKSITVNIENSTAWIQSGATLGELYYNIAQKSRTLAFPAGVCPTVG 180
           YVA+LPFII+DLINL+S+T+++ENSTAW+QSGATLGELYY I +KSRTLAFPAGVCPTVG
Sbjct: 121 YVAYLPFIIVDLINLRSVTIDVENSTAWVQSGATLGELYYRIGEKSRTLAFPAGVCPTVG 180

Query: 181 VGGHFSGGGYGLMLRKYGLAADNVIDAYLIDANGKFYDRESMGEDLFWAIRGGGGGSFGI 240
           VGGHFSGGGYG MLRK+GLAADNVIDAYL+DANGK +DRESMGEDLFWAIRGGGGGSFGI
Sbjct: 181 VGGHFSGGGYGFMLRKFGLAADNVIDAYLVDANGKVHDRESMGEDLFWAIRGGGGGSFGI 240

Query: 241 VVAWKVKLVPVPAIVTTCSSDRNLDEDTINLIHRWQSVAYKLDENLYFGIILAGGN-AST 300
           VVAWKVKLVPVPA VT+CS+ RNL+ D I L+H+WQ VA KLDENL+ GI+L GGN  +T
Sbjct: 241 VVAWKVKLVPVPATVTSCSTHRNLEGDAIKLVHQWQYVANKLDENLFLGILLTGGNITTT 300

Query: 301 EGGVKKPNPTATFFSLFLGKSDELMAILNTNFPELNLAKKDCVETSWIESTLVAATRNQT 360
           + G+   +PTATFF LFLGK DEL+A L+T FPEL+L K+DCVETSWIESTL+AAT  Q 
Sbjct: 301 QEGIA--DPTATFFILFLGKVDELVATLSTTFPELSLMKQDCVETSWIESTLIAATGVQI 360

Query: 361 IESLESLLNRTPLTIENTKLKSDYVKEPIPKATIEGIWERLKAQDIEVPQLVFVPYGGRM 420
           +ESLE LLNRTP TIE+TK+KSDY+KEPIP+ATIEGI++RLKAQDI   QL+FVPYGGRM
Sbjct: 361 VESLEPLLNRTPPTIESTKIKSDYIKEPIPEATIEGIFQRLKAQDIATSQLIFVPYGGRM 420

Query: 421 SRISDSETPFSHRARNLYKIGYIVGWKEQSVSAEERHLSWIREIYDYMTPFVSKSPRATY 480
           S+IS+SETPFSHRA NLYKIGYIVGWKEQS++A++RH+SWIRE+Y+YMTPFVSKSPR+ Y
Sbjct: 421 SQISESETPFSHRAANLYKIGYIVGWKEQSLNAKKRHISWIREVYEYMTPFVSKSPRSAY 480

Query: 481 VNYRDLDIGTNNKYGKTSYKQASVWGSKYFGNNFNRLVHIKSKVDPSNFFKNEQSIPPL 537
            NYRDLDIG NNKY KTS KQASVWG KYFGNNF RLV++K+KVDP +FF++EQSIP L
Sbjct: 481 ANYRDLDIGANNKYRKTSVKQASVWGLKYFGNNFKRLVYVKTKVDPYDFFRHEQSIPAL 537

BLAST of MC03g_new0285 vs. NCBI nr
Match: XP_038895543.1 (berberine bridge enzyme-like 18 [Benincasa hispida])

HSP 1 Score: 830 bits (2145), Expect = 1.46e-299
Identity = 404/538 (75.09%), Postives = 473/538 (87.92%), Query Frame = 0

Query: 1   MKYS-PLTILSLAFILLSSSSSWAAASAEKHEAFLQCLSHHSADSSSISKVIYTPINPSY 60
           MK+S PL  L+L  I+L S   WAAAS +K++ FLQCLS HS++S  ISKV+Y+PIN SY
Sbjct: 1   MKFSSPLIPLALVLIVLYSCPLWAAASPQKYQHFLQCLSEHSSNSYPISKVVYSPINSSY 60

Query: 61  SSVLNFSVRNLRFSSAETPKPLLIITPSYVSHVQAAVICSKTHGLQIRIRSGGHDIEGLS 120
           SSVL+FS++NLRFS  +TPKPLLIITPS+VSH+QAAVICSK+HGLQIR RSGGHD EGLS
Sbjct: 61  SSVLDFSIKNLRFSKPQTPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGLS 120

Query: 121 YVAHLPFIIIDLINLKSITVNIENSTAWIQSGATLGELYYNIAQKSRTLAFPAGVCPTVG 180
           YVA+ PFI++DLINL+SI+V+ EN+TAW++SGATLGELYY I +KSRTLAFPAGVCPTVG
Sbjct: 121 YVAYRPFIVVDLINLRSISVDDENNTAWVESGATLGELYYRIGEKSRTLAFPAGVCPTVG 180

Query: 181 VGGHFSGGGYGLMLRKYGLAADNVIDAYLIDANGKFYDRESMGEDLFWAIRGGGGGSFGI 240
           +GGHFSGGGYGLMLRK+GLAADNVIDA+L+DANGK +DRESMGEDLFWAIRGGGGGSFGI
Sbjct: 181 IGGHFSGGGYGLMLRKFGLAADNVIDAHLVDANGKVHDRESMGEDLFWAIRGGGGGSFGI 240

Query: 241 VVAWKVKLVPVPAIVTTCSSDRNLDEDTINLIHRWQSVAYKLDENLYFGIILAGGNASTE 300
           VVAWK+KLVPVPA VT CS+ RNL+ED I L+HRWQ VA KLDENLY GIIL GG AST+
Sbjct: 241 VVAWKIKLVPVPATVTICSTQRNLEEDAIKLVHRWQYVANKLDENLYLGIILTGGKASTQ 300

Query: 301 GGVKKPNPTATFFSLFLGKSDELMAILNTNFPELNLAKKDCVETSWIESTLVAATRNQTI 360
           GG+   NPTATFFSLFLG+ DEL+A L+T FPEL L K+DCVE +W+ESTL+ AT  Q I
Sbjct: 301 GGIT--NPTATFFSLFLGRIDELLATLSTTFPELGLIKQDCVEATWVESTLIIATGVQPI 360

Query: 361 ESLESLLNRTPLTIENTKLKSDYVKEPIPKATIEGIWERLKAQDIEVPQLVFVPYGGRMS 420
           ESLE LLNRTP TIENTK+KSDYVKEP+P+A IEGI +RLKAQDIE  QL+FVPYGG+MS
Sbjct: 361 ESLEPLLNRTPPTIENTKIKSDYVKEPLPEAAIEGICQRLKAQDIETSQLIFVPYGGKMS 420

Query: 421 RISDSETPFSHRARNLYKIGYIVGWKEQSVSAEERHLSWIREIYDYMTPFVSKSPRATYV 480
           +IS+SETPFSHRA NLYKIGY+VGWKEQS+ A++RH+ WIRE+Y+YM PFVSKSPRA Y 
Sbjct: 421 QISESETPFSHRAGNLYKIGYVVGWKEQSLKAKKRHIHWIREVYEYMIPFVSKSPRAAYA 480

Query: 481 NYRDLDIGTNNKYGKTSYKQASVWGSKYFGNNFNRLVHIKSKVDPSNFFKNEQSIPPL 537
           NYRDLDIG NNKYG+TS +QASVWG KYFGNNF +LV++K+KVDP +FF++EQSIP L
Sbjct: 481 NYRDLDIGANNKYGQTSVEQASVWGLKYFGNNFKKLVYVKTKVDPYDFFRHEQSIPTL 536

BLAST of MC03g_new0285 vs. NCBI nr
Match: XP_023520318.1 (berberine bridge enzyme-like 18 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 821 bits (2120), Expect = 8.94e-296
Identity = 396/531 (74.58%), Postives = 467/531 (87.95%), Query Frame = 0

Query: 5   PLTILSLAFILLSSSSSWAAASAEKHEAFLQCLSHHSADSSSISKVIYTPINPSYSSVLN 64
           PLT++ +  I+LSSS  WA+ S   H+ FL CLSHHS  +  ISK++YTPIN SYSSVLN
Sbjct: 5   PLTLIFI--IVLSSSPLWASPSLHIHQHFLHCLSHHSPHTYPISKLVYTPINSSYSSVLN 64

Query: 65  FSVRNLRFSSAETPKPLLIITPSYVSHVQAAVICSKTHGLQIRIRSGGHDIEGLSYVAHL 124
           FS+RNL FS+ +TPKPLLIITPS++SH+QAAVICSK H LQIR RSGGHD EGLSYVA  
Sbjct: 65  FSIRNLIFSNPQTPKPLLIITPSHISHIQAAVICSKAHDLQIRTRSGGHDFEGLSYVADQ 124

Query: 125 PFIIIDLINLKSITVNIENSTAWIQSGATLGELYYNIAQKSRTLAFPAGVCPTVGVGGHF 184
           PFII+DLINL+SI V+++ +TAW+QSGATLGELYY I +KSR+LAFPAG+CPTVGVGGHF
Sbjct: 125 PFIIVDLINLRSINVDVQTNTAWVQSGATLGELYYRIGEKSRSLAFPAGICPTVGVGGHF 184

Query: 185 SGGGYGLMLRKYGLAADNVIDAYLIDANGKFYDRESMGEDLFWAIRGGGGGSFGIVVAWK 244
           SGGGYGLMLRKYGLAADNV+DAYL+DANG+ +DRESMGEDLFWAIRGGGGGSFGIVVAWK
Sbjct: 185 SGGGYGLMLRKYGLAADNVVDAYLVDANGRVHDRESMGEDLFWAIRGGGGGSFGIVVAWK 244

Query: 245 VKLVPVPAIVTTCSSDRNLDEDTINLIHRWQSVAYKLDENLYFGIILAGGNASTEGGVKK 304
           VKLV VPA+VT CS++R++++D I LIHRWQ VA +LD+NL+ GI+L GGN ST  G+  
Sbjct: 245 VKLVVVPAVVTMCSTNRDVEDDAIKLIHRWQYVANQLDDNLFLGILLTGGNFSTRQGIT- 304

Query: 305 PNPTATFFSLFLGKSDELMAILNTNFPELNLAKKDCVETSWIESTLVAATRNQTIESLES 364
            NPTATFFSL+LGK DEL+AIL T FPEL L KKDCVE+SWI+STL+A+T  QT E LE+
Sbjct: 305 -NPTATFFSLYLGKLDELIAILKTTFPELGLTKKDCVESSWIQSTLIASTGVQTFEPLEA 364

Query: 365 LLNRTPLTIENTKLKSDYVKEPIPKATIEGIWERLKAQDIEVPQLVFVPYGGRMSRISDS 424
           LLNRTP TIE+TK KSDYVK+PIP+A IEGIW+RLKAQDIEVPQ++F+PYGGRMS+IS+S
Sbjct: 365 LLNRTPPTIESTKFKSDYVKDPIPEAAIEGIWQRLKAQDIEVPQILFIPYGGRMSQISES 424

Query: 425 ETPFSHRARNLYKIGYIVGWKEQSVSAEERHLSWIREIYDYMTPFVSKSPRATYVNYRDL 484
           ETPFSHRA NLYKIGY V WKEQS+ A+ RH++WIRE+ ++MTPFVSKSPRA YVNYRDL
Sbjct: 425 ETPFSHRAGNLYKIGYAVSWKEQSLDAKNRHINWIREVNEHMTPFVSKSPRAAYVNYRDL 484

Query: 485 DIGTNNKYGKTSYKQASVWGSKYFGNNFNRLVHIKSKVDPSNFFKNEQSIP 535
           DIGTNNKYGKTSY+QASVWG KYFGNNFNRLV++K+KVDP +FF++EQSIP
Sbjct: 485 DIGTNNKYGKTSYEQASVWGMKYFGNNFNRLVYVKTKVDPHDFFRHEQSIP 531

BLAST of MC03g_new0285 vs. ExPASy TrEMBL
Match: A0A6J1DF27 (berberine bridge enzyme-like 8 OS=Momordica charantia OX=3673 GN=LOC111020224 PE=3 SV=1)

HSP 1 Score: 1068 bits (2762), Expect = 0.0
Identity = 537/537 (100.00%), Postives = 537/537 (100.00%), Query Frame = 0

Query: 1   MKYSPLTILSLAFILLSSSSSWAAASAEKHEAFLQCLSHHSADSSSISKVIYTPINPSYS 60
           MKYSPLTILSLAFILLSSSSSWAAASAEKHEAFLQCLSHHSADSSSISKVIYTPINPSYS
Sbjct: 1   MKYSPLTILSLAFILLSSSSSWAAASAEKHEAFLQCLSHHSADSSSISKVIYTPINPSYS 60

Query: 61  SVLNFSVRNLRFSSAETPKPLLIITPSYVSHVQAAVICSKTHGLQIRIRSGGHDIEGLSY 120
           SVLNFSVRNLRFSSAETPKPLLIITPSYVSHVQAAVICSKTHGLQIRIRSGGHDIEGLSY
Sbjct: 61  SVLNFSVRNLRFSSAETPKPLLIITPSYVSHVQAAVICSKTHGLQIRIRSGGHDIEGLSY 120

Query: 121 VAHLPFIIIDLINLKSITVNIENSTAWIQSGATLGELYYNIAQKSRTLAFPAGVCPTVGV 180
           VAHLPFIIIDLINLKSITVNIENSTAWIQSGATLGELYYNIAQKSRTLAFPAGVCPTVGV
Sbjct: 121 VAHLPFIIIDLINLKSITVNIENSTAWIQSGATLGELYYNIAQKSRTLAFPAGVCPTVGV 180

Query: 181 GGHFSGGGYGLMLRKYGLAADNVIDAYLIDANGKFYDRESMGEDLFWAIRGGGGGSFGIV 240
           GGHFSGGGYGLMLRKYGLAADNVIDAYLIDANGKFYDRESMGEDLFWAIRGGGGGSFGIV
Sbjct: 181 GGHFSGGGYGLMLRKYGLAADNVIDAYLIDANGKFYDRESMGEDLFWAIRGGGGGSFGIV 240

Query: 241 VAWKVKLVPVPAIVTTCSSDRNLDEDTINLIHRWQSVAYKLDENLYFGIILAGGNASTEG 300
           VAWKVKLVPVPAIVTTCSSDRNLDEDTINLIHRWQSVAYKLDENLYFGIILAGGNASTEG
Sbjct: 241 VAWKVKLVPVPAIVTTCSSDRNLDEDTINLIHRWQSVAYKLDENLYFGIILAGGNASTEG 300

Query: 301 GVKKPNPTATFFSLFLGKSDELMAILNTNFPELNLAKKDCVETSWIESTLVAATRNQTIE 360
           GVKKPNPTATFFSLFLGKSDELMAILNTNFPELNLAKKDCVETSWIESTLVAATRNQTIE
Sbjct: 301 GVKKPNPTATFFSLFLGKSDELMAILNTNFPELNLAKKDCVETSWIESTLVAATRNQTIE 360

Query: 361 SLESLLNRTPLTIENTKLKSDYVKEPIPKATIEGIWERLKAQDIEVPQLVFVPYGGRMSR 420
           SLESLLNRTPLTIENTKLKSDYVKEPIPKATIEGIWERLKAQDIEVPQLVFVPYGGRMSR
Sbjct: 361 SLESLLNRTPLTIENTKLKSDYVKEPIPKATIEGIWERLKAQDIEVPQLVFVPYGGRMSR 420

Query: 421 ISDSETPFSHRARNLYKIGYIVGWKEQSVSAEERHLSWIREIYDYMTPFVSKSPRATYVN 480
           ISDSETPFSHRARNLYKIGYIVGWKEQSVSAEERHLSWIREIYDYMTPFVSKSPRATYVN
Sbjct: 421 ISDSETPFSHRARNLYKIGYIVGWKEQSVSAEERHLSWIREIYDYMTPFVSKSPRATYVN 480

Query: 481 YRDLDIGTNNKYGKTSYKQASVWGSKYFGNNFNRLVHIKSKVDPSNFFKNEQSIPPL 537
           YRDLDIGTNNKYGKTSYKQASVWGSKYFGNNFNRLVHIKSKVDPSNFFKNEQSIPPL
Sbjct: 481 YRDLDIGTNNKYGKTSYKQASVWGSKYFGNNFNRLVHIKSKVDPSNFFKNEQSIPPL 537

BLAST of MC03g_new0285 vs. ExPASy TrEMBL
Match: A0A6J1DG89 (tetrahydrocannabinolic acid synthase-like OS=Momordica charantia OX=3673 GN=LOC111020225 PE=3 SV=1)

HSP 1 Score: 882 bits (2279), Expect = 0.0
Identity = 435/537 (81.01%), Postives = 480/537 (89.39%), Query Frame = 0

Query: 1   MKYSPLTILSLAFILLSSSSSWAAASAEKHEAFLQCLSHHSADSSSISKVIYTPINPSYS 60
           MKYSPL ILSLAFILLSSSSSWA A AEKHE+FLQCL HHS++SSSISKVIY+PIN SYS
Sbjct: 1   MKYSPLIILSLAFILLSSSSSWAVACAEKHESFLQCLFHHSSNSSSISKVIYSPINSSYS 60

Query: 61  SVLNFSVRNLRFSSAETPKPLLIITPSYVSHVQAAVICSKTHGLQIRIRSGGHDIEGLSY 120
           SVLN SV+N RF +A+TPKPL IITPS++SH+Q AVICSKTHG QIR RS GHD EGLSY
Sbjct: 61  SVLNSSVQNFRFLTAKTPKPLFIITPSHLSHIQIAVICSKTHGFQIRTRSDGHDTEGLSY 120

Query: 121 VAHLPFIIIDLINLKSITVNIENSTAWIQSGATLGELYYNIAQKSRTLAFPAGVCPTVGV 180
           VA++PFIIIDLINL+S+T+N+E+STAW+QSGATLGELYY+IAQKS+TLAFPAGVCPTVGV
Sbjct: 121 VAYVPFIIIDLINLRSVTINVEHSTAWVQSGATLGELYYSIAQKSQTLAFPAGVCPTVGV 180

Query: 181 GGHFSGGGYGLMLRKYGLAADNVIDAYLIDANGKFYDRESMGEDLFWAIRGGGGGSFGIV 240
           GGH SGGGYGLMLRKYG AADNVIDAYLIDANGKF+DRESMGEDLFWAIRGGGGGS GIV
Sbjct: 181 GGHSSGGGYGLMLRKYGFAADNVIDAYLIDANGKFHDRESMGEDLFWAIRGGGGGSLGIV 240

Query: 241 VAWKVKLVPVPAIVTTCSSDRNLDEDTINLIHRWQSVAYKLDENLYFGIILAGGNASTEG 300
           VAWKVKLVPVPA VT C ++RNLDED + LIHRWQ VA KLDENLY GI+L GGNAST+G
Sbjct: 241 VAWKVKLVPVPATVTICLTNRNLDEDLVKLIHRWQFVAPKLDENLYVGIMLNGGNASTQG 300

Query: 301 GVKKPNPTATFFSLFLGKSDELMAILNTNFPELNLAKKDCVETSWIESTLVAATRNQTIE 360
           GV   NP +TFFSLFLGK DELMAIL  +FPE+NLA  DCVETSWIEST++AATRN+T+E
Sbjct: 301 GV---NPKSTFFSLFLGKIDELMAILKRDFPEMNLANNDCVETSWIESTIIAATRNKTVE 360

Query: 361 SLESLLNRTPLTIENTKLKSDYVKEPIPKATIEGIWERLKAQDIEVPQLVFVPYGGRMSR 420
           SLE LL + PL   + K KSDYVKEPIPK  IEGIWERLKAQDIE  QLV VPYGGRMS+
Sbjct: 361 SLEYLLTKIPLATGSIKTKSDYVKEPIPKVAIEGIWERLKAQDIEASQLVIVPYGGRMSQ 420

Query: 421 ISDSETPFSHRARNLYKIGYIVGWKEQSVSAEERHLSWIREIYDYMTPFVSKSPRATYVN 480
           I DSE P+SHRA  LYKIGY+VGWKEQSV   +RHLSWIRE+YDYMTPFVSKSPRA YVN
Sbjct: 421 ILDSEIPYSHRAGYLYKIGYVVGWKEQSVDVGKRHLSWIRELYDYMTPFVSKSPRAAYVN 480

Query: 481 YRDLDIGTNNKYGKTSYKQASVWGSKYFGNNFNRLVHIKSKVDPSNFFKNEQSIPPL 537
           YRDLDIGTNNKYGKTSYK+AS+WGSKYFG NFNRLVH+KSKVDPSNFF++EQSIPPL
Sbjct: 481 YRDLDIGTNNKYGKTSYKRASIWGSKYFGKNFNRLVHVKSKVDPSNFFRHEQSIPPL 534

BLAST of MC03g_new0285 vs. ExPASy TrEMBL
Match: A0A6J1KHF6 (berberine bridge enzyme-like 18 OS=Cucurbita maxima OX=3661 GN=LOC111495284 PE=3 SV=1)

HSP 1 Score: 820 bits (2118), Expect = 8.73e-296
Identity = 392/531 (73.82%), Postives = 466/531 (87.76%), Query Frame = 0

Query: 5   PLTILSLAFILLSSSSSWAAASAEKHEAFLQCLSHHSADSSSISKVIYTPINPSYSSVLN 64
           PLT++ +  I+LSSS  WA+ S  KH+ FL CLSHHS  +  ISK++YTPIN SYS VLN
Sbjct: 5   PLTLIFI--IVLSSSPLWASPSLHKHQHFLHCLSHHSPHTYPISKLVYTPINSSYSPVLN 64

Query: 65  FSVRNLRFSSAETPKPLLIITPSYVSHVQAAVICSKTHGLQIRIRSGGHDIEGLSYVAHL 124
           FS+RNL FS+ +TPKPLLIITPS++SH+QAA++CSK H LQIR RSGGHD EGLSYVA+ 
Sbjct: 65  FSIRNLIFSNPQTPKPLLIITPSHISHIQAAIVCSKAHDLQIRTRSGGHDFEGLSYVANQ 124

Query: 125 PFIIIDLINLKSITVNIENSTAWIQSGATLGELYYNIAQKSRTLAFPAGVCPTVGVGGHF 184
           PFII+DLINL+SI V+++ +TAW+QSGATLGELYY I + SR+LAFPAG+CPTVGVGGHF
Sbjct: 125 PFIIVDLINLRSINVDVQTNTAWVQSGATLGELYYRIGETSRSLAFPAGICPTVGVGGHF 184

Query: 185 SGGGYGLMLRKYGLAADNVIDAYLIDANGKFYDRESMGEDLFWAIRGGGGGSFGIVVAWK 244
           SGGGYGLMLRKYGLAADNV+DAYL+DANG+ +DRESMGEDLFWAIRGGGGGSFGIVVAWK
Sbjct: 185 SGGGYGLMLRKYGLAADNVVDAYLVDANGRVHDRESMGEDLFWAIRGGGGGSFGIVVAWK 244

Query: 245 VKLVPVPAIVTTCSSDRNLDEDTINLIHRWQSVAYKLDENLYFGIILAGGNASTEGGVKK 304
           VKLV VPA+VT CS++R++++D I LIHRWQ VA +LD+NL+ GI+L GGN ST  G+  
Sbjct: 245 VKLVMVPAVVTMCSTNRDVEDDAIKLIHRWQYVANQLDDNLFLGILLTGGNFSTREGIT- 304

Query: 305 PNPTATFFSLFLGKSDELMAILNTNFPELNLAKKDCVETSWIESTLVAATRNQTIESLES 364
            NPTATFFSL+LGK DEL+ IL  NF EL L KKDCVE+SWI+STL+A+T  QT E LE+
Sbjct: 305 -NPTATFFSLYLGKLDELITILKNNFSELGLTKKDCVESSWIQSTLIASTGVQTFEPLEA 364

Query: 365 LLNRTPLTIENTKLKSDYVKEPIPKATIEGIWERLKAQDIEVPQLVFVPYGGRMSRISDS 424
           LLNRTP TIE+TK KSDYVK+PIP+ATIEGIW+RLKAQDIEVPQ++F+PYGGRMS+IS+S
Sbjct: 365 LLNRTPPTIESTKFKSDYVKDPIPEATIEGIWQRLKAQDIEVPQILFIPYGGRMSQISES 424

Query: 425 ETPFSHRARNLYKIGYIVGWKEQSVSAEERHLSWIREIYDYMTPFVSKSPRATYVNYRDL 484
           ETPFSHRA NLYKIGY V WKEQS+ A+ +H++WIRE+Y+YMTPFVSKSPRA Y NYRDL
Sbjct: 425 ETPFSHRAGNLYKIGYAVSWKEQSLDAKNKHINWIREVYEYMTPFVSKSPRAAYANYRDL 484

Query: 485 DIGTNNKYGKTSYKQASVWGSKYFGNNFNRLVHIKSKVDPSNFFKNEQSIP 535
           DIGTNNKYGKTSY+QASVWG KYFGNNFNRLV++K+KVDP +FF++EQSIP
Sbjct: 485 DIGTNNKYGKTSYEQASVWGMKYFGNNFNRLVYVKTKVDPHDFFRHEQSIP 531

BLAST of MC03g_new0285 vs. ExPASy TrEMBL
Match: A0A6J1CN68 (tetrahydrocannabinolic acid synthase-like OS=Momordica charantia OX=3673 GN=LOC111012666 PE=3 SV=1)

HSP 1 Score: 819 bits (2115), Expect = 2.90e-295
Identity = 402/537 (74.86%), Postives = 471/537 (87.71%), Query Frame = 0

Query: 4   SPLTILSLAFILLSSSSSWAAASAEKHEAFLQCLSHHSADSS--SISKVIYTPINPSYSS 63
           SPL  LSLAFI LSSSS WA+A   KHE+FLQCL  HS + S  SISKVI+TPIN SYSS
Sbjct: 5   SPLISLSLAFIFLSSSSLWASAFPNKHESFLQCLLDHSLEYSNYSISKVIHTPINSSYSS 64

Query: 64  VLNFSVRNLRFSSAETPKPLLIITPSYVSHVQAAVICSKTHGLQIRIRSGGHDIEGLSYV 123
           VL+FS+RNLRFS+ ETPKPLLI+TPS+VSH+QAAVICSK++G QIR RSGGHD EGLSYV
Sbjct: 65  VLDFSIRNLRFSTVETPKPLLIVTPSHVSHIQAAVICSKSYGFQIRTRSGGHDFEGLSYV 124

Query: 124 AHLPFIIIDLINLKSITVNIENSTAWIQSGATLGELYYNIAQKSRTLAFPAGVCPTVGVG 183
           AHLPFII+DLINL+SI+++I+++TAW+QSGATLGELYY+IAQKSRTLAFPAG+CPTVGVG
Sbjct: 125 AHLPFIIVDLINLRSISIDIKDNTAWVQSGATLGELYYSIAQKSRTLAFPAGICPTVGVG 184

Query: 184 GHFSGGGYGLMLRKYGLAADNVIDAYLIDANGKFYDRESMGEDLFWAIRGGGGGSFGIVV 243
           GHFSGGGYGL+LRKYGLAADNVIDAYL+DA+GKF++RESMGEDLFWAIRGGGGGSFGIVV
Sbjct: 185 GHFSGGGYGLLLRKYGLAADNVIDAYLVDASGKFHNRESMGEDLFWAIRGGGGGSFGIVV 244

Query: 244 AWKVKLVPVPAIVTTCSSDRNLDEDTINLIHRWQSVAYKLDENLYFGIILAGGN-ASTEG 303
           AWKVKLVPVPA VT CS++R L+E  + + HRWQ VA KLDENL+ GI+  GG   S EG
Sbjct: 245 AWKVKLVPVPATVTLCSANRTLEEGAVKITHRWQYVANKLDENLFLGILWLGGKITSQEG 304

Query: 304 GVKKPNPTATFFSLFLGKSDELMAILNTNFPELNLAKKDCVETSWIESTLVAATRNQTIE 363
           G  K NP ATFFSLFLGK+DEL++ILNT FPEL+LAKKDC+ETSWIEST++      T  
Sbjct: 305 G--KTNPVATFFSLFLGKADELLSILNTKFPELDLAKKDCIETSWIESTVLMGVGFPTEL 364

Query: 364 SLESLLNRTPLTIENTKLKSDYVKEPIPKATIEGIWERLKAQDIEVPQLVFVPYGGRMSR 423
           +LE+LL+RTPLT  ++K+KSDYVKEP+ +A ++GIW+RL +QDIE   ++FVPYGGRMS+
Sbjct: 365 TLEALLSRTPLTNTSSKIKSDYVKEPMSEAVVQGIWKRLDSQDIEGGNIIFVPYGGRMSQ 424

Query: 424 ISDSETPFSHRARNLYKIGYIVGWKEQSVSAEERHLSWIREIYDYMTPFVSKSPRATYVN 483
           IS+SETPFSHRA  LYKIGYI GW+EQ + AE+RHL WIRE+YDYMTPFVSKSPRA Y N
Sbjct: 425 ISESETPFSHRAGYLYKIGYIAGWEEQGIDAEKRHLRWIRELYDYMTPFVSKSPRAAYAN 484

Query: 484 YRDLDIGTNNKYGKTSYKQASVWGSKYFGNNFNRLVHIKSKVDPSNFFKNEQSIPPL 537
           YRDLDIG+NNK+GKTSYKQASVWG KYFGNNFNRLVH+K+KVDP +FF++EQSIP L
Sbjct: 485 YRDLDIGSNNKFGKTSYKQASVWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQSIPAL 539

BLAST of MC03g_new0285 vs. ExPASy TrEMBL
Match: A0A5A7TU57 (Tetrahydrocannabinolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1317G00190 PE=3 SV=1)

HSP 1 Score: 816 bits (2107), Expect = 4.28e-294
Identity = 396/538 (73.61%), Postives = 467/538 (86.80%), Query Frame = 0

Query: 1   MKYSPLTI-LSLAFILLSSSSSWAAASAEKHEAFLQCLSHHSADSSSISKVIYTPINPSY 60
           MK+S   I LSL FI++SS+S  A+AS +K++ FLQCLS HS+ S  ISKV+YTPIN SY
Sbjct: 1   MKFSSTLIPLSLVFIVVSSASLCASASPQKYQNFLQCLSEHSSKSYPISKVVYTPINSSY 60

Query: 61  SSVLNFSVRNLRFSSAETPKPLLIITPSYVSHVQAAVICSKTHGLQIRIRSGGHDIEGLS 120
           SSVL+FS+RNL FS  ETPKPLLIITPS+VSH+QAAVICSK+HGLQIR RSGGHD EGLS
Sbjct: 61  SSVLDFSIRNLLFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGLS 120

Query: 121 YVAHLPFIIIDLINLKSITVNIENSTAWIQSGATLGELYYNIAQKSRTLAFPAGVCPTVG 180
           YVA+ PFI++DLINL+S+ V +EN+TAW++SGATLGELYY I +KSRTLAFPAGVCPTVG
Sbjct: 121 YVAYRPFIVVDLINLRSVKVEVENNTAWVESGATLGELYYRIGEKSRTLAFPAGVCPTVG 180

Query: 181 VGGHFSGGGYGLMLRKYGLAADNVIDAYLIDANGKFYDRESMGEDLFWAIRGGGGGSFGI 240
           +GGHFSGGGYGLMLRK+GLAADNVIDAYL+DANGK  D+ESMGEDLFWAIRGGGGGSFGI
Sbjct: 181 IGGHFSGGGYGLMLRKFGLAADNVIDAYLVDANGKVLDKESMGEDLFWAIRGGGGGSFGI 240

Query: 241 VVAWKVKLVPVPAIVTTCSSDRNLDEDTINLIHRWQSVAYKLDENLYFGIILAGGNASTE 300
           VVAWK+KLVPVP  VT CS+DRNL+ED I LIHRWQ V  KLDEN+Y GIIL GGNAST+
Sbjct: 241 VVAWKIKLVPVPETVTICSTDRNLEEDAIKLIHRWQYVGNKLDENIYLGIILTGGNASTQ 300

Query: 301 GGVKKPNPTATFFSLFLGKSDELMAILNTNFPELNLAKKDCVETSWIESTLVAATRNQTI 360
            G+   NPTA FFSLFLG++DEL+  L+T FPEL L K+DCVE SW+ESTL+     Q I
Sbjct: 301 AGIT--NPTARFFSLFLGRADELVTTLSTIFPELGLIKQDCVEASWVESTLIIPIGVQPI 360

Query: 361 ESLESLLNRTPLTIENTKLKSDYVKEPIPKATIEGIWERLKAQDIEVPQLVFVPYGGRMS 420
           E+LE LLNRT  T+++TK+KSDY+ EPIP A I+ IW+RLK QDIE  QLVFVPYGGRMS
Sbjct: 361 ETLEPLLNRTLTTLDSTKIKSDYITEPIPVAAIKDIWQRLKTQDIETSQLVFVPYGGRMS 420

Query: 421 RISDSETPFSHRARNLYKIGYIVGWKEQSVSAEERHLSWIREIYDYMTPFVSKSPRATYV 480
           +IS+SETPFSHRAR L+KIGY+VGWK+QS+ A++RH+SW+RE+Y+YM PFVSKSPRA Y 
Sbjct: 421 QISESETPFSHRARYLFKIGYVVGWKDQSLKAKKRHISWLREVYEYMAPFVSKSPRAAYT 480

Query: 481 NYRDLDIGTNNKYGKTSYKQASVWGSKYFGNNFNRLVHIKSKVDPSNFFKNEQSIPPL 537
           NYRDLDIG+NNKYGKTSYK+ASVWG KYFGNNF+RLV++K+KVDP +FF++EQSIP L
Sbjct: 481 NYRDLDIGSNNKYGKTSYKRASVWGLKYFGNNFDRLVYVKTKVDPYDFFRHEQSIPTL 536

BLAST of MC03g_new0285 vs. TAIR 10
Match: AT1G30700.1 (FAD-binding Berberine family protein )

HSP 1 Score: 525.4 bits (1352), Expect = 5.4e-149
Identity = 267/542 (49.26%), Postives = 368/542 (67.90%), Query Frame = 0

Query: 1   MKYSPLTILSLAFILLSSSSSWAAASAEKHEAFLQCLSHHSADSSSISKVIYTPINPSYS 60
           MKY+ + +L     +  SSSS A       E F QCL+ +S     IS  I+   N SYS
Sbjct: 1   MKYALILVLFFVVFIWQSSSSSA-----NSETFTQCLTSNSDPKHPISPAIFFSGNGSYS 60

Query: 61  SVLNFSVRNLRFSSAETPKPLLIITPSYVSHVQAAVICSKTHGLQIRIRSGGHDIEGLSY 120
           SVL  ++RNLRF++  TPKP LII  ++ SHVQAA+ C K H LQ++IRSGGHD +GLSY
Sbjct: 61  SVLQANIRNLRFNTTSTPKPFLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGLSY 120

Query: 121 VAH--LPFIIIDLINLKSITVNIENSTAWIQSGATLGELYYNIAQKSRTLAFPAGVCPTV 180
           V +   PF ++D+ NL+S+ V++ + TAW+Q+GA LGE+YY I +KS+TLA+PAG+CPTV
Sbjct: 121 VTYSGKPFFVLDMFNLRSVDVDVASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGICPTV 180

Query: 181 GVGGHFSGGGYGLMLRKYGLAADNVIDAYLIDANGKFYDRESMGEDLFWAIRGGGGGSFG 240
           GVGGH SGGGYG M+RKYGL  DN IDA ++D NGK  DR+ MGEDL+WAI GGGGGS+G
Sbjct: 181 GVGGHISGGGYGNMMRKYGLTVDNTIDARMVDVNGKILDRKLMGEDLYWAINGGGGGSYG 240

Query: 241 IVVAWKVKLVPVPAIVTTCSSDRNLDEDTINLIHRWQSVAYKLDENLYFGIIL--AGGNA 300
           +V+A+K+ LV VP  VT     R L+++  ++IHRWQ VA KL + L+   ++    G  
Sbjct: 241 VVLAYKINLVEVPENVTVFRISRTLEQNATDIIHRWQQVAPKLPDELFIRTVIDVVNGTV 300

Query: 301 STEGGVKKPNPTATFFSLFLGKSDELMAILNTNFPELNLAKKDCVETSWIESTLVAATRN 360
           S++  V+      TF ++FLG +  L++ILN  FPEL L + DC ETSWI+S L     N
Sbjct: 301 SSQKTVR-----TTFIAMFLGDTTTLLSILNRRFPELGLVRSDCTETSWIQSVLF--WTN 360

Query: 361 QTIESLESLLNRTPLTIENTKLKSDYVKEPIPKATIEGIWERLKAQDIEVPQLVFVPYGG 420
             + S E+LL +    +   K KSDYV+EPI +  +E IW+  K  ++E+P + F PYGG
Sbjct: 361 IQVGSSETLLLQRNQPVNYLKRKSDYVREPISRTGLESIWK--KMIELEIPTMAFNPYGG 420

Query: 421 RMSRISDSETPFSHRARNLYKIGYIVGWKEQSVSAEERHLSWIREIYDYMTPFVSKSPRA 480
            M RIS + TPF +RA NL+KI Y   W++++++  +R++   R++Y +MTPFVSK+PR 
Sbjct: 421 EMGRISSTVTPFPYRAGNLWKIQYGANWRDETLT--DRYMELTRKLYQFMTPFVSKNPRQ 480

Query: 481 TYVNYRDLDIGTNNKYGK-TSYKQASVWGSKYFGNNFNRLVHIKSKVDPSNFFKNEQSIP 538
           ++ NYRD+D+G N+  GK +SY +   +G KYF  NF RLV IK++VD  NFF+NEQSIP
Sbjct: 481 SFFNYRDVDLGINSHNGKISSYVEGKRYGKKYFAGNFERLVKIKTRVDSGNFFRNEQSIP 526

BLAST of MC03g_new0285 vs. TAIR 10
Match: AT4G20820.1 (FAD-binding Berberine family protein )

HSP 1 Score: 521.5 bits (1342), Expect = 7.8e-148
Identity = 261/534 (48.88%), Postives = 361/534 (67.60%), Query Frame = 0

Query: 3   YSPLTILSLAFILLSSSSSWAAASAEKHEAFLQCLSHHSADSSSISKVIYTPINPSYSSV 62
           +S + I      LL S     +A+     +FLQCLS    DS+ +SKVI+TP + S+SSV
Sbjct: 7   FSSVLIFFTTSTLLLSIPHPVSANRSNQSSFLQCLSLQLNDSNIVSKVIHTPNDTSFSSV 66

Query: 63  LNFSVRNLRFSSAETPKPLLIITPSYVSHVQAAVICSKTHGLQIRIRSGGHDIEGLSYVA 122
           L  S++N RFS+ + PKP+LI+TP   S VQ+AV C++  G+ IR RSGGHD EGLSYV 
Sbjct: 67  LASSIQNQRFSAPDVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSYVT 126

Query: 123 HLPFIIIDLINLKSITVNIENSTAWIQSGATLGELYYNIAQKSRTLAFPAGVCPTVGVGG 182
           H PF+I+DL NL+SITV+++N + W+Q+GAT+GELYY I +K+RTLAFPAGVCPTVGVGG
Sbjct: 127 HKPFVILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGVGG 186

Query: 183 HFSGGGYGLMLRKYGLAADNVIDAYLIDANGKFYDRESMGEDLFWAIRGGGGGSFGIVVA 242
           HFSGGGYG +LRK+GLAAD+VIDA ++DA G+  +R  MGED FWAIRGGGG SF +V++
Sbjct: 187 HFSGGGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGGGSSFCVVLS 246

Query: 243 WKVKLVPVPAIVTTCSSDRNLDEDTINLIHRWQSVAYKLDENLYFGIILAGGNASTEGGV 302
           WK+ L+ VP+ VT  +  +  ++  + +IHRWQ VA K+ ++L+  ++L           
Sbjct: 247 WKIGLINVPSTVTVFNVTKFSEQSALKIIHRWQFVADKVSDDLFIRVMLQR--------- 306

Query: 303 KKPNPTATFFSLFLGKSDELMAILNTNFPELNLAKKDCVETSWIESTLVAATRNQTIESL 362
            K    A+F  L+LG    L+ ++N  FPEL L + DC E SWIES +  A   +  E +
Sbjct: 307 YKNMVRASFPGLYLGSVKNLLKMVNKEFPELGLEEDDCTEMSWIESVIWFAELGE--EPI 366

Query: 363 ESLLNRTPLTIENTKLKSDYVKEPIPKATIEGIWERLKAQDIEVPQLVFVPYGGRMSRIS 422
             L  RT  ++   K KSD+V+EP+PK  I  +W RL+  + E  QL+F P+GG+MS I+
Sbjct: 367 NVLTKRTRASLA-FKAKSDFVQEPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGKMSEIA 426

Query: 423 DSETPFSHRARNLYKIGYIVGWKEQSVSAEERHLSWIREIYDYMTPFVSKSPRATYVNYR 482
           D ETPF HR  N+Y+I Y+  W+      +E+++ W+  +YD M+ FV+KSPR  Y+N R
Sbjct: 427 DYETPFPHRKGNIYEIQYLNYWRG---DVKEKYMRWVERVYDDMSEFVAKSPRGAYINLR 486

Query: 483 DLDIGTNNKYGKTSYKQASVWGSKYFGNNFNRLVHIKSKVDPSNFFKNEQSIPP 537
           DLD+G      ++ Y++   WG KYF NNF RLV +K+ VDPS+FF +EQSIPP
Sbjct: 487 DLDLGMYVGVKRSKYEEGKSWGVKYFKNNFERLVRVKTSVDPSDFFCDEQSIPP 525

BLAST of MC03g_new0285 vs. TAIR 10
Match: AT5G44410.1 (FAD-binding Berberine family protein )

HSP 1 Score: 506.9 bits (1304), Expect = 2.0e-143
Identity = 255/538 (47.40%), Postives = 369/538 (68.59%), Query Frame = 0

Query: 6   LTILSLAFILLS-SSSSWAAASAEK--HEAFLQCLSHHSADSSSISKVIYTPINPSYSSV 65
           L  L + F+LL+ S S + + SA++  HE FL+CLSH   +    S++I+T  +PSY S+
Sbjct: 7   LLSLFIYFLLLNLSLSHFPSISAQRTNHENFLKCLSHRINEDD--SRIIHTSKDPSYFSI 66

Query: 66  LNFSVRNLRFSSAETPKPLLIITPSYVSHVQAAVICSKTHGLQIRIRSGGHDIEGLSYVA 125
           LN S++N RF   ETPKP+ IITP   + VQ+ + C++ HG+ IR RSGGHD EGLSY+A
Sbjct: 67  LNSSIQNPRFFVLETPKPVSIITPVQATDVQSTIKCARLHGIHIRTRSGGHDYEGLSYMA 126

Query: 126 -HLPFIIIDLINLKSITVNIENSTAWIQSGATLGELYYNIAQKSRTLAFPAGVCPTVGVG 185
              PF++IDL NL+SIT++++N T W+QSGAT+GELYY I + S++LAFPAG+ PTVG+G
Sbjct: 127 KSRPFVVIDLRNLRSITLDVDNRTGWVQSGATIGELYYEIGKLSKSLAFPAGLYPTVGIG 186

Query: 186 GHFSGGGYGLMLRKYGLAADNVIDAYLIDANGKFYDRESMGEDLFWAIRGGGGGSFGIVV 245
           G F GGGYG ++RKYGL+ADNVIDA+++DANG F DR+ MGED FWAIRGGGG SF +V+
Sbjct: 187 GQFGGGGYGTLMRKYGLSADNVIDAHIVDANGSFLDRQGMGEDFFWAIRGGGGSSFSVVL 246

Query: 246 AWKVKLVPVPAIVTTCSSDRNLDEDTINLIHRWQSVAYKLDENLYFGIILAGGNASTEGG 305
           +WK++L+ VP++VT     +  +++ +++I++WQ +A K+  +L+   +L          
Sbjct: 247 SWKIRLLDVPSVVTVFKVVKTSEKEAVSIINKWQYIADKVPNDLFIRAMLQ--------- 306

Query: 306 VKKPNPTATFFSLFLGKSDELMAILNTNFPELNLAKKDCVETSWIESTLVAATRNQTIES 365
            K+    A+F  L+LG   +L+A++   FPEL L   +C E SWIES L         ES
Sbjct: 307 -KETEVYASFPGLYLGPVSDLLALMKDKFPELGLEIGNCREMSWIESVLWFIKG----ES 366

Query: 366 LESLLNRTPLTIENTKLKSDYVKEPIPKATIEGIWERLKAQDIEVPQLVFVPYGGRMSRI 425
           +E L  R   T  + K K D+++EPIPK  I+ +W R +A +  + +++  P+GG+MS I
Sbjct: 367 MEILAKR-KRTSRSFKGKDDFIEEPIPKTAIQYLWRRFEAPEARLAKIILTPFGGKMSEI 426

Query: 426 SDSETPFSHRARNLYKIGYIVGWKEQ---SVSAEERHLSWIREIYDYMTPFVSKSPRATY 485
           +D+E PF HR  NLY+I Y+  W E+   + +  E++L W+  +Y++MTP+VSKSPR  Y
Sbjct: 427 ADNEIPFPHREGNLYEIQYLAYWSEEEDKNKTNTEKYLRWVESVYEFMTPYVSKSPRRAY 486

Query: 486 VNYRDLDIGTNNKYG-KTSYKQASVWGSKYFGNNFNRLVHIKSKVDPSNFFKNEQSIP 536
           VN+RD+D+G       KT Y++A VWG KYF NNF+RLV +K+ VDP +FF +EQSIP
Sbjct: 487 VNFRDIDLGMYLGLNMKTKYEEAKVWGVKYFKNNFDRLVRVKTNVDPMDFFCDEQSIP 527

BLAST of MC03g_new0285 vs. TAIR 10
Match: AT5G44400.1 (FAD-binding Berberine family protein )

HSP 1 Score: 501.9 bits (1291), Expect = 6.4e-142
Identity = 253/536 (47.20%), Postives = 360/536 (67.16%), Query Frame = 0

Query: 7   TILSLAFILLSSSSSWAAASAEKHEAFLQCLSHHSADSSSISKVIYTPIN--PSYSSVLN 66
           +IL L F L + + + + AS +  + F+ C+  ++     + K  + P      +S VL 
Sbjct: 11  SILVLYFSLYTITPTSSLASLQ--DQFINCVQRNTHVYFPLEKTFFAPTKNVSMFSQVLE 70

Query: 67  FSVRNLRFSSAETPKPLLIITPSYVSHVQAAVICSKTHGLQIRIRSGGHDIEGLSYVAHL 126
            + +NLRF     PKP  I +P + SHVQA++ICSK   + +R+RSGGHD EGLSYV+ +
Sbjct: 71  STAQNLRFLKKSMPKPGFIFSPIHESHVQASIICSKKLRMHLRVRSGGHDYEGLSYVSQI 130

Query: 127 --PFIIIDLINLKSITVNIENSTAWIQSGATLGELYYNIAQKSRTLAFPAGVCPTVGVGG 186
             PFI++DL  ++ + +NI++++AW+QSGAT+GELYY IA+KS+   FPAG+C ++G+GG
Sbjct: 131 DKPFILMDLSKMRQVNINIQDNSAWVQSGATVGELYYRIAEKSKVHGFPAGLCSSLGIGG 190

Query: 187 HFSGGGYGLMLRKYGLAADNVIDAYLIDANGKFYDRESMGEDLFWAIRGGGGGSFGIVVA 246
           H +GG YG M+RKYGL ADNV+DA ++DANGK  DR +MGED FWAIRGG GGSFGI++A
Sbjct: 191 HITGGAYGSMMRKYGLGADNVLDAKIVDANGKLLDRAAMGEDTFWAIRGGAGGSFGIILA 250

Query: 247 WKVKLVPVPAIVTTCSSDRNLDEDTIN-LIHRWQSVAYKLDENLYFGIILAGGNASTEGG 306
           WK+KLVPVP  VT  +  + L +D  N +I +WQ VA KL E L+  ++    N +  GG
Sbjct: 251 WKIKLVPVPKTVTVFTVTKTLQQDVGNKIISKWQRVADKLVEELFIRVLF---NVAGTGG 310

Query: 307 VKKPNPTATFFSLFLGKSDELMAILNTNFPELNLAKKDCVETSWIESTLVAATRNQTIES 366
            K    T ++ +LFLG    LM ++  +FPEL L  KDC+E SW+ES    +       +
Sbjct: 311 NK--TVTTSYNALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESIAYISGFPTHTPT 370

Query: 367 LESLLNRTPLTIENTKLKSDYVKEPIPKATIEGIWERLKAQDIEVPQLVFVPYGGRMSRI 426
              L  ++P    + K KSD+VK PIP++ ++GI+++L  +DI  P +++ PYGG M++I
Sbjct: 371 NVLLQGKSPFPKVSFKAKSDFVKTPIPESGLQGIFKKLLKEDI--PLMIWNPYGGMMAKI 430

Query: 427 SDSETPFSHRARNLYKIGYIVGWKEQSVSAEERHLSWIREIYDYMTPFVSKSPRATYVNY 486
            +S+ PF HR   L+K+ Y+  W + S     RH++WIR++Y YMTP+VS +PR  YVNY
Sbjct: 431 PESQIPFPHRKGVLFKVQYVTSWLD-SDKRPSRHINWIRDLYSYMTPYVSSNPREAYVNY 490

Query: 487 RDLDIGTNNKYGKTSYKQASVWGSKYFGNNFNRLVHIKSKVDPSNFFKNEQSIPPL 538
           RDLD+G N K  KT  KQA VWG+ YF NNFNRL+ IK+KVDP NFF++EQSIPP+
Sbjct: 491 RDLDLGRNTKDVKTCIKQAQVWGANYFKNNFNRLMMIKAKVDPENFFRHEQSIPPM 536

BLAST of MC03g_new0285 vs. TAIR 10
Match: AT5G44440.1 (FAD-binding Berberine family protein )

HSP 1 Score: 500.4 bits (1287), Expect = 1.9e-141
Identity = 250/539 (46.38%), Postives = 364/539 (67.53%), Query Frame = 0

Query: 1   MKYSPLTILSLAFILLSSSSSWAAASAEKHEAFLQCLSHHSADSSSISKVIYTPINPSYS 60
           M++S      L F L  + S   +A    HE FL+CLS+   D++   KVI+T  + S+ 
Sbjct: 1   MEFSSFLFTILLFSL--NISPLVSAHGSNHEDFLKCLSYRMNDNTVEPKVIHTSKDSSFF 60

Query: 61  SVLNFSVRNLRFSSAETPKPLLIITPSYVSHVQAAVICSKTHGLQIRIRSGGHDIEGLSY 120
           S+L+ S++N RFS +ETPKP+ IITP   S VQ  + C++ HG+ +R RS GH  EGLSY
Sbjct: 61  SILDSSIQNPRFSVSETPKPVSIITPVKASDVQTVIRCAQLHGIHVRTRSAGHCYEGLSY 120

Query: 121 VAH-LPFIIIDLINLKSITVNIENSTAWIQSGATLGELYYNIAQKSRTLAFPAGVCPTVG 180
           +A+  PF +IDL NL+SI+++++N T W+Q+GAT GELYY I + +++LAFPAG+ PTVG
Sbjct: 121 IAYNKPFAVIDLRNLRSISLDVDNRTGWVQTGATAGELYYEIGKTTKSLAFPAGIHPTVG 180

Query: 181 VGGHFSGGGYGLMLRKYGLAADNVIDAYLIDANGKFYDRESMGEDLFWAIRGGGGGSFGI 240
           VGG FSGGGYG +LRKYGLAADN+IDA ++DA+G+  DR++MGED FWAIRGGGG SFG+
Sbjct: 181 VGGQFSGGGYGTLLRKYGLAADNIIDALVVDASGRILDRQAMGEDYFWAIRGGGGSSFGV 240

Query: 241 VVAWKVKLVPVPAIVTTCSSDRNLDEDTINLIHRWQSVAYKLDENLYFGIILAGGNASTE 300
           +++WKVKLV VP+ +T     +   ++ + +I +WQ  A K+ ++L+    L   N    
Sbjct: 241 ILSWKVKLVDVPSTITVFKVQKTSKKEAVRIIKKWQYAADKVPDDLFIRTTLERSN---- 300

Query: 301 GGVKKPNPTATFFSLFLGKSDELMAILNTNFPELNLAKKDCVETSWIESTLVAATRNQTI 360
               K    A F  L++G  + L+A++   FPEL L K+ C E SWIES L  A   +  
Sbjct: 301 ----KNAVHALFTGLYIGPVNNLLALMEEKFPELGLEKEGCEEMSWIESVLWFADFPKG- 360

Query: 361 ESLESLLNRTPLTIENTKLKSDYVKEPIPKATIEGIWERLKAQDIEVPQLVFVPYGGRMS 420
           ESL  L NR   ++ + K K D+V+EPIP+A I+ IW RL+A +  + +++  P+GG+MS
Sbjct: 361 ESLGVLTNRERTSL-SFKGKDDFVQEPIPEAAIQEIWRRLEAPEARLGKIILTPFGGKMS 420

Query: 421 RISDSETPFSHRARNLYKIGYIVGWKEQ---SVSAEERHLSWIREIYDYMTPFVSKSPRA 480
            +++ ETPF HR  NLY+I Y+  W+E+   + +  +++L W+  +Y++MTP+VSKSPR 
Sbjct: 421 EMAEYETPFPHRGGNLYEIQYVAYWREEEDKNKTETDKYLKWVDSVYEFMTPYVSKSPRG 480

Query: 481 TYVNYRDLDIGTNNKYGKTSYKQASVWGSKYFGNNFNRLVHIKSKVDPSNFFKNEQSIP 536
            YVN++D+D+G      KT Y++   WG KYF NNF RLV +K++VDP++FF +EQSIP
Sbjct: 481 AYVNFKDMDLGMYLGKKKTKYEEGKSWGVKYFKNNFERLVRVKTRVDPTDFFCDEQSIP 527