MC03g_new0281 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC03g_new0281
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	berberine bridge enzyme-like 18
Location	MC03: 12796718 .. 12798491 (-)
RNA-Seq Expression	MC03g_new0281
Synteny	MC03g_new0281

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAGGTGAAGTTGGTTCCGTTCCGACGACTGTGACTATTTGCTCAACTAACAGAACTTTGGAGGAAGGTGCAGTGAAGCTAATGCATCGGTGGCAATATGTTGCCAACAAATTGGACGAAAATGTGTTGCTGAACATCCTTTGGCTTGGTATTCTTTCAACTTTTTTGCTTGAGTTATTTCTTATTTATTAATTCCTTTTCCGTGAAATTTATCCTTTGTGACCTCGTATTGAATAGATAAAAGAGTACATGAATAAACAAATACATAGGGAAAGAGTGATCTTAAAACTAACTTTTGATTGAGTAGAATTAAGGACTCGCAATTTTTATTTTATTGGAATAAACATTATATTAAGACTTTTGATGCCAGAGTGAGCATAGTTCAGTCGTAATAATTGACATATATCTTCAATCAAGAGATTGGGAGTTTGAATCCCCTCCTCCATGTTCTATTAAAAAAAATATATACTGAGACTTTTGATTTTGTACAATTATACATCTATCTTATGTTTGAGTAGGGTATAAATTTACAACTTAAATTAAGACTTGTAGACTTTATTTTTTGACAATTTTGTATCTTATGTTTAAGAAGGGTATTTAAATTCACAACTTATTTAAATTCATTCTTTAGTCTAATGTAATTGGAAACGCTAACATACAAATTTAGTCCATGAAGTTTTAAAGTTGAGTTTTTTTTTTCTTTTTTAAAAAAAGAGTTTTTTAAGTTTAAAAAGTGTCAAGTATGTCCAATACCTACTTTGTGTTTAGTAAGTTCCTAAATTTTGAAAGAATATATTACTGTCTTTTTAAATTTCAATTTGTCTGTTTAATAAATCCTTCATTTGTTCAAAAATTTATTAGATTCATGAACTAGTTATACACACAAGAAATTTGTGTTTAATTATTCGACGTTGACTTTTCGATTTTGTGTTTAGCAGGTTTATGAATTTCAAGAAATGTCTAATAGATTTAGGTTAATAATTTAACCAAATTAGATAAAATAAACTTAAAGACTGAGAAAAGGAAAACATAAACCTAAAAATAAATAAATAAATAAAACTATCACTCCGTCCAAGGTTTTAAATGTTGATATAAATGGAAATGTTGAGGTCTCAATTTCACGGAAATATCGATAAAATGTCAATGTCCATGGACATTTCTGTAAAAGTTATAGAAATTAAAAAATCGTAAAGAGTATTTAAATTAACAAATAAATTTTTTTTATATTTCTAATTAATTAAAAATGATTGCTCCTAATATTAATACTATATATATTGGTGACATTTGTTGATATTTTTCATATTTAATCAATTTTTTTGTTATACGATGGGAATGTCGATTTACATCTCATGTCAATGTCGTCTAACTCGTGGATACATAGAAATAAGATGAAAATATCGACGCGTTGACAGATATTTAATACATGATTCCATGGCATATAGACATATGAAATGAGTTTTTTCCTAAAAACAAAATCCCATCCCAATACATAAAAATTTGGATGATTTGAACTCAATTCTTGATGGTAGGTGGGAATATCACAGGTCAAGGAGGAGGCAAAATAAACCCAATAGCTACATTCTCCTCTTTTTTTCTTGGAAAGGCAGATGAGCTTCTTGCAATCTTGAAGAAAACTTTTCCAGAGCTACATTTGGCGAAGAAAGAATGCACAGAAACGAGTTGGATTCAATCAACTGTCCTCGTGGAAGCTATGCTCAGTAGAACACCTCTCGCCAACATAAGCTTAAAAATCAAATCTGACTTCGTCTAG

mRNA sequence

ATGGAAGGTGAAGTTGGTTCCGTTCCGACGACTGTGACTATTTGCTCAACTAACAGAACTTTGGAGGAAGGTGCAGTGAAGCTAATGCATCGGTGGCAATATGTTGCCAACAAATTGGACGAAAATGTGTTGCTGAACATCCTTTGGCTTGGTGGGAATATCACAGGTCAAGGAGGAGGCAAAATAAACCCAATAGCTACATTCTCCTCTTTTTTTCTTGGAAAGGCAGATGAGCTTCTTGCAATCTTGAAGAAAACTTTTCCAGAGCTACATTTGGCGAAGAAAGAATGCACAGAAACGAGTTGGATTCAATCAACTGTCCTCGTGGAAGCTATGCTCAGTAGAACACCTCTCGCCAACATAAGCTTAAAAATCAAATCTGACTTCGTCTAG

Coding sequence (CDS)

ATGGAAGGTGAAGTTGGTTCCGTTCCGACGACTGTGACTATTTGCTCAACTAACAGAACTTTGGAGGAAGGTGCAGTGAAGCTAATGCATCGGTGGCAATATGTTGCCAACAAATTGGACGAAAATGTGTTGCTGAACATCCTTTGGCTTGGTGGGAATATCACAGGTCAAGGAGGAGGCAAAATAAACCCAATAGCTACATTCTCCTCTTTTTTTCTTGGAAAGGCAGATGAGCTTCTTGCAATCTTGAAGAAAACTTTTCCAGAGCTACATTTGGCGAAGAAAGAATGCACAGAAACGAGTTGGATTCAATCAACTGTCCTCGTGGAAGCTATGCTCAGTAGAACACCTCTCGCCAACATAAGCTTAAAAATCAAATCTGACTTCGTCTAG

Protein sequence

MEGEVGSVPTTVTICSTNRTLEEGAVKLMHRWQYVANKLDENVLLNILWLGGNITGQGGGKINPIATFSSFFLGKADELLAILKKTFPELHLAKKECTETSWIQSTVLVEAMLSRTPLANISLKIKSDFV

Homology

[Close]

BLAST of MC03g_new0281 vs. ExPASy Swiss-Prot
Match: O64743 (Berberine bridge enzyme-like 15 OS=Arabidopsis thaliana OX=3702 GN=MEE23 PE=1 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 1.5e-17
Identity = 46/132 (34.85%), Postives = 82/132 (62.12%), Query Frame = 0


Query: 8   VPTTVTICSTNRTLEEGAVKLMHRWQYVANKLDENVLLNILWLGGNITGQGGGKINPIAT 67
           VP TVT+ +  +TLE+   K++++W+ +A+KLD+++ + ++    + T + G +   + +
Sbjct: 254 VPATVTVFTVTKTLEQDGTKVLYKWEQIADKLDDDLFIRVIISPASKTTKPGNRTISM-S 313

Query: 68  FSSFFLGKADELLAILKKTFPELHLAKKECTETSWIQSTVLV---------EAMLSRTPL 127
           + + FLG ++ LL +++K+FPEL L KK+CTE SWI+S + +         EA+L+   L
Sbjct: 314 YQAQFLGDSNRLLQVMQKSFPELGLTKKDCTEMSWIKSVMYIAGFPNSAAPEALLAGKSL 373

Query: 128 ANISLKIKSDFV 131
                K KSDFV
Sbjct: 374 FKNHFKAKSDFV 384

[Close]

BLAST of MC03g_new0281 vs. ExPASy Swiss-Prot
Match: Q93ZA3 (Berberine bridge enzyme-like 13 OS=Arabidopsis thaliana OX=3702 GN=At1g30760 PE=1 SV=1)

HSP 1 Score: 87.0 bits (214), Expect = 1.7e-16
Identity = 50/133 (37.59%), Postives = 79/133 (59.40%), Query Frame = 0


Query: 8   VPTTVTICSTNRTLEEGAVKLMHRWQYVANKLDENVLLNILWLGGNITGQGGGKINPIAT 67
           VP  VT+ +  RTLE+   KL+++WQ VA+KLDE++ + ++    + T +   K   I+T
Sbjct: 256 VPEIVTVFTVTRTLEQDGTKLLYKWQQVADKLDEDLFIRVIIQPTSKTPK--SKERTIST 315

Query: 68  -FSSFFLGKADELLAILKKTFPELHLAKKECTETSWIQSTVLV---------EAMLSRTP 127
            +   FLG A+ LL +++++FP+L L KK+C ETSWI+S + +         EA+L    
Sbjct: 316 SYQGQFLGDANRLLQVMQRSFPQLGLTKKDCLETSWIKSVMYIAGFPSTAPSEALLDGKS 375

Query: 128 LANISLKIKSDFV 131
           L     K KSD+V
Sbjct: 376 LFKNYFKAKSDYV 386

[Close]

BLAST of MC03g_new0281 vs. ExPASy Swiss-Prot
Match: Q9FZC7 (Berberine bridge enzyme-like 6 OS=Arabidopsis thaliana OX=3702 GN=FOX4 PE=2 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 8.3e-16
Identity = 45/132 (34.09%), Postives = 74/132 (56.06%), Query Frame = 0


Query: 8   VPTTVTICSTNRTLEEGAVKLMHRWQYVANKLDENVLLNILWLGGNITGQGGGKINPIAT 67
           VP  +T+   N+TLE+G   ++++WQ VANKL +++ +   W    + G   G+      
Sbjct: 270 VPKILTVFKVNKTLEQGGTDILYKWQLVANKLPDSLFIT-AW-PRTVNGPKPGERTVAVV 329

Query: 68  FSSFFLGKADELLAILKKTFPELHLAKKECTETSWIQSTVL---------VEAMLSRTPL 127
           F + FLG  D+L+ I+ ++FPEL L +++C E SW+ +T+             +L R P 
Sbjct: 330 FYAQFLGPTDKLMEIMDQSFPELGLGREDCHEMSWLNTTLFWANYPAGTPKSILLDRPPT 389

Query: 128 ANISLKIKSDFV 131
            ++S K KSDFV
Sbjct: 390 NSVSFKSKSDFV 399

[Close]

BLAST of MC03g_new0281 vs. ExPASy Swiss-Prot
Match: Q9FKU8 (Berberine bridge enzyme-like 26 OS=Arabidopsis thaliana OX=3702 GN=At5g44400 PE=2 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 1.8e-15
Identity = 46/134 (34.33%), Postives = 76/134 (56.72%), Query Frame = 0


Query: 8   VPTTVTICSTNRTLEEG-AVKLMHRWQYVANKLDENVLLNILWLGGNITGQGGGKINPIA 67
           VP TVT+ +  +TL++    K++ +WQ VA+KL E + + +L+   N+ G GG K     
Sbjct: 256 VPKTVTVFTVTKTLQQDVGNKIISKWQRVADKLVEELFIRVLF---NVAGTGGNK-TVTT 315

Query: 68  TFSSFFLGKADELLAILKKTFPELHLAKKECTETSWIQSTVLVEA----------MLSRT 127
           ++++ FLG    L+ ++KK+FPEL L  K+C E SW++S   +            +  ++
Sbjct: 316 SYNALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESIAYISGFPTHTPTNVLLQGKS 375

Query: 128 PLANISLKIKSDFV 131
           P   +S K KSDFV
Sbjct: 376 PFPKVSFKAKSDFV 385

[Close]

BLAST of MC03g_new0281 vs. ExPASy Swiss-Prot
Match: Q9SA85 (Berberine bridge enzyme-like 8 OS=Arabidopsis thaliana OX=3702 GN=At1g30700 PE=2 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 1.6e-14
Identity = 52/133 (39.10%), Postives = 74/133 (55.64%), Query Frame = 0


Query: 8   VPTTVTICSTNRTLEEGAVKLMHRWQYVANKLDENVLLN--ILWLGGNITGQGGGKINPI 67
           VP  VT+   +RTLE+ A  ++HRWQ VA KL + + +   I  + G ++ Q   +    
Sbjct: 247 VPENVTVFRISRTLEQNATDIIHRWQQVAPKLPDELFIRTVIDVVNGTVSSQKTVR---- 306

Query: 68  ATFSSFFLGKADELLAILKKTFPELHLAKKECTETSWIQSTVL---VEAMLSRTPLANIS 127
            TF + FLG    LL+IL + FPEL L + +CTETSWIQS +    ++   S T L   +
Sbjct: 307 TTFIAMFLGDTTTLLSILNRRFPELGLVRSDCTETSWIQSVLFWTNIQVGSSETLLLQRN 366

Query: 128 -----LKIKSDFV 131
                LK KSD+V
Sbjct: 367 QPVNYLKRKSDYV 375

[Close]

BLAST of MC03g_new0281 vs. NCBI nr
Match: XP_022142425.1 (berberine bridge enzyme-like 18 [Momordica charantia])

HSP 1 Score: 194 bits (494), Expect = 1.66e-56
Identity = 97/133 (72.93%), Postives = 107/133 (80.45%), Query Frame = 0


Query: 8   VPTTVTICSTNRTLEEGAVKLMHRWQYVANKLDENVLLNILWLGGNITGQGGGKINPIAT 67
           VP TVT+C TNRTLEEGAVKL HRWQYVANKLDEN+ L ILWLGGN+T QGGGK NP+AT
Sbjct: 254 VPATVTLCQTNRTLEEGAVKLTHRWQYVANKLDENLFLGILWLGGNVTSQGGGKTNPVAT 313

Query: 68  FSSFFLGKADELLAILKKTFPELHLAKKECTETSWIQSTVLV----------EAMLSRTP 127
           F S FLG+ADELLAIL   FPEL LAKK+C ETSWI+STVL+          EA+LSRTP
Sbjct: 314 FFSLFLGQADELLAILNTKFPELDLAKKDCIETSWIESTVLMGIGFQTKVTLEALLSRTP 373

Query: 128 LANISLKIKSDFV 130
           L NIS KIKSD+V
Sbjct: 374 LTNISTKIKSDYV 386

[Close]

BLAST of MC03g_new0281 vs. NCBI nr
Match: XP_022142587.1 (tetrahydrocannabinolic acid synthase-like [Momordica charantia])

HSP 1 Score: 186 bits (473), Expect = 1.27e-53
Identity = 94/133 (70.68%), Postives = 104/133 (78.20%), Query Frame = 0


Query: 8   VPTTVTICSTNRTLEEGAVKLMHRWQYVANKLDENVLLNILWLGGNITGQGGGKINPIAT 67
           VP TVT+CS NRTLEEGAVK+ HRWQYVANKLDEN+ L ILWLGG IT Q GGK NP+AT
Sbjct: 253 VPATVTLCSANRTLEEGAVKITHRWQYVANKLDENLFLGILWLGGKITSQEGGKTNPVAT 312

Query: 68  FSSFFLGKADELLAILKKTFPELHLAKKECTETSWIQSTVLV----------EAMLSRTP 127
           F S FLGKADELL+IL   FPEL LAKK+C ETSWI+STVL+          EA+LSRTP
Sbjct: 313 FFSLFLGKADELLSILNTKFPELDLAKKDCIETSWIESTVLMGVGFPTELTLEALLSRTP 372

Query: 128 LANISLKIKSDFV 130
           L N S KIKSD+V
Sbjct: 373 LTNTSSKIKSDYV 385

[Close]

BLAST of MC03g_new0281 vs. NCBI nr
Match: XP_022152516.1 (berberine bridge enzyme-like 18 [Momordica charantia])

HSP 1 Score: 168 bits (425), Expect = 1.38e-46
Identity = 85/132 (64.39%), Postives = 99/132 (75.00%), Query Frame = 0


Query: 8   VPTTVTICSTNRTLEEGAVKLMHRWQYVANKLDENVLLNILWLGGNITGQGGGKINPIAT 67
           VP TVT C  NRTLEEGA+KL+HRWQYVANKLDEN+ L IL +GGN T QGG K NP A+
Sbjct: 254 VPATVTSCYYNRTLEEGAIKLIHRWQYVANKLDENLFLGILLMGGNFTAQGGDKTNPTAS 313

Query: 68  FSSFFLGKADELLAILKKTFPELHLAKKECTETSWIQSTVL---------VEAMLSRTPL 127
           F S FLGKADE  AIL  TFPEL LAK+ C E+SWI+S +L         +EA+L+RTPL
Sbjct: 314 FFSLFLGKADEFFAILNTTFPELGLAKENCLESSWIESALLSAGFKIGDSLEALLNRTPL 373

Query: 128 ANISLKIKSDFV 130
            N S+KIKSD+V
Sbjct: 374 TNGSIKIKSDYV 385

[Close]

BLAST of MC03g_new0281 vs. NCBI nr
Match: XP_038895343.1 (cannabidiolic acid synthase-like [Benincasa hispida])

HSP 1 Score: 157 bits (397), Expect = 1.63e-42
Identity = 82/134 (61.19%), Postives = 100/134 (74.63%), Query Frame = 0


Query: 8   VPTTVTICSTNRTLEEGAVKLMHRWQYVANKLDENVLLNILWLGGNITGQGGGKINPIAT 67
           VP TVTIC+ NRT +EGA+KL+ +WQYVANKLDE++ L+I+  GG I+ Q GGKINP A 
Sbjct: 253 VPATVTICTANRTWKEGAIKLIDQWQYVANKLDEDLFLSIILTGGKISAQ-GGKINPTAL 312

Query: 68  FSSFFLGKADELLAILKKTFPELHLAKKECTETSWIQSTVL-----------VEAMLSRT 127
           F + FLGKADELL ILK  FPEL+LAK++C ETSWI+S V            +EA L+RT
Sbjct: 313 FFTLFLGKADELLTILKNKFPELNLAKEDCKETSWIESAVYTANGLQIDDQPLEAFLNRT 372

Query: 128 PLANISLKIKSDFV 130
           PLAN S KIKSD+V
Sbjct: 373 PLANGSFKIKSDYV 385

[Close]

BLAST of MC03g_new0281 vs. NCBI nr
Match: KAE8653318.1 (hypothetical protein Csa_023332 [Cucumis sativus])

HSP 1 Score: 151 bits (381), Expect = 2.14e-42
Identity = 76/129 (58.91%), Postives = 96/129 (74.42%), Query Frame = 0


Query: 7   SVPTTVTICSTNRTLEEGAVKLMHRWQYVANKLDENVLLNILWLGGNITGQGGGKINPIA 66
           SVPTTVTIC+T R +EEGA+ L+H+WQYVANK DE++ L I+  GG I+ + GGKINPIA
Sbjct: 137 SVPTTVTICTTTRNMEEGAINLIHQWQYVANKFDEDLFLGIILTGGKISAE-GGKINPIA 196

Query: 67  TFSSFFLGKADELLAILKKTFPELHLAKKECTETSWIQSTVLV-----------EAMLSR 124
            F + FLGKADEL+AIL KTFPEL L K+EC ETSWI+ST+ V           + +L+R
Sbjct: 197 LFFTLFLGKADELMAILSKTFPELGLTKEECKETSWIESTIFVASGFQMGDQASDVLLNR 256

[Close]

BLAST of MC03g_new0281 vs. ExPASy TrEMBL
Match: A0A6J1CKW8 (berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111012562 PE=3 SV=1)

HSP 1 Score: 194 bits (494), Expect = 8.06e-57
Identity = 97/133 (72.93%), Postives = 107/133 (80.45%), Query Frame = 0


Query: 8   VPTTVTICSTNRTLEEGAVKLMHRWQYVANKLDENVLLNILWLGGNITGQGGGKINPIAT 67
           VP TVT+C TNRTLEEGAVKL HRWQYVANKLDEN+ L ILWLGGN+T QGGGK NP+AT
Sbjct: 254 VPATVTLCQTNRTLEEGAVKLTHRWQYVANKLDENLFLGILWLGGNVTSQGGGKTNPVAT 313

Query: 68  FSSFFLGKADELLAILKKTFPELHLAKKECTETSWIQSTVLV----------EAMLSRTP 127
           F S FLG+ADELLAIL   FPEL LAKK+C ETSWI+STVL+          EA+LSRTP
Sbjct: 314 FFSLFLGQADELLAILNTKFPELDLAKKDCIETSWIESTVLMGIGFQTKVTLEALLSRTP 373

Query: 128 LANISLKIKSDFV 130
           L NIS KIKSD+V
Sbjct: 374 LTNISTKIKSDYV 386

[Close]

BLAST of MC03g_new0281 vs. ExPASy TrEMBL
Match: A0A6J1CN68 (tetrahydrocannabinolic acid synthase-like OS=Momordica charantia OX=3673 GN=LOC111012666 PE=3 SV=1)

HSP 1 Score: 186 bits (473), Expect = 6.13e-54
Identity = 94/133 (70.68%), Postives = 104/133 (78.20%), Query Frame = 0


Query: 8   VPTTVTICSTNRTLEEGAVKLMHRWQYVANKLDENVLLNILWLGGNITGQGGGKINPIAT 67
           VP TVT+CS NRTLEEGAVK+ HRWQYVANKLDEN+ L ILWLGG IT Q GGK NP+AT
Sbjct: 253 VPATVTLCSANRTLEEGAVKITHRWQYVANKLDENLFLGILWLGGKITSQEGGKTNPVAT 312

Query: 68  FSSFFLGKADELLAILKKTFPELHLAKKECTETSWIQSTVLV----------EAMLSRTP 127
           F S FLGKADELL+IL   FPEL LAKK+C ETSWI+STVL+          EA+LSRTP
Sbjct: 313 FFSLFLGKADELLSILNTKFPELDLAKKDCIETSWIESTVLMGVGFPTELTLEALLSRTP 372

Query: 128 LANISLKIKSDFV 130
           L N S KIKSD+V
Sbjct: 373 LTNTSSKIKSDYV 385

[Close]

BLAST of MC03g_new0281 vs. ExPASy TrEMBL
Match: A0A6J1DHZ2 (berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020223 PE=3 SV=1)

HSP 1 Score: 168 bits (425), Expect = 6.70e-47
Identity = 85/132 (64.39%), Postives = 99/132 (75.00%), Query Frame = 0


Query: 8   VPTTVTICSTNRTLEEGAVKLMHRWQYVANKLDENVLLNILWLGGNITGQGGGKINPIAT 67
           VP TVT C  NRTLEEGA+KL+HRWQYVANKLDEN+ L IL +GGN T QGG K NP A+
Sbjct: 254 VPATVTSCYYNRTLEEGAIKLIHRWQYVANKLDENLFLGILLMGGNFTAQGGDKTNPTAS 313

Query: 68  FSSFFLGKADELLAILKKTFPELHLAKKECTETSWIQSTVL---------VEAMLSRTPL 127
           F S FLGKADE  AIL  TFPEL LAK+ C E+SWI+S +L         +EA+L+RTPL
Sbjct: 314 FFSLFLGKADEFFAILNTTFPELGLAKENCLESSWIESALLSAGFKIGDSLEALLNRTPL 373

Query: 128 ANISLKIKSDFV 130
            N S+KIKSD+V
Sbjct: 374 TNGSIKIKSDYV 385

[Close]

BLAST of MC03g_new0281 vs. ExPASy TrEMBL
Match: A0A6J1DEU9 (berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020168 PE=3 SV=1)

HSP 1 Score: 154 bits (388), Expect = 1.57e-41
Identity = 75/132 (56.82%), Postives = 98/132 (74.24%), Query Frame = 0


Query: 8   VPTTVTICSTNRTLEEGAVKLMHRWQYVANKLDENVLLNILWLGGNITGQGGGKINPIAT 67
           VPTT+T C+ NRTL+   VKL+HRWQYVA+KL++N+LL I   GG+I  Q GGK NP+A+
Sbjct: 252 VPTTLTTCTVNRTLDRDGVKLIHRWQYVAHKLEDNLLLGISLTGGHILTQQGGKTNPVAS 311

Query: 68  FSSFFLGKADELLAILKKTFPELHLAKKECTETSWIQSTVL---------VEAMLSRTPL 127
           F ++FLGK DEL+AI+  TFPEL L K++C ETSWI+ST++         +E +L R PL
Sbjct: 312 FPTYFLGKVDELVAIVNTTFPELGLTKEDCIETSWIESTLITAGFSAGESLETLLYRRPL 371

Query: 128 ANISLKIKSDFV 130
            NIS KIKSD+V
Sbjct: 372 VNISTKIKSDYV 383

[Close]

BLAST of MC03g_new0281 vs. ExPASy TrEMBL
Match: A0A5A7TYU2 (Cannabidiolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1317G00260 PE=3 SV=1)

HSP 1 Score: 153 bits (387), Expect = 2.16e-41
Identity = 81/134 (60.45%), Postives = 99/134 (73.88%), Query Frame = 0


Query: 8   VPTTVTICSTNRTLEEGAVKLMHRWQYVANKLDENVLLNILWLGGNITGQGGGKINPIAT 67
           VP TVTIC+ +RTLEE A+KL+ +WQYVANKLDE++ L I  LGG I  Q G KINPIA 
Sbjct: 252 VPATVTICTISRTLEEEAIKLIDQWQYVANKLDEDLFLGINLLGGKIVAQ-GDKINPIAL 311

Query: 68  FSSFFLGKADELLAILKKTFPELHLAKKECTETSWIQSTVL-----------VEAMLSRT 127
           F S FLGKADEL+AIL KTFP+L L K+EC ETSWI+S V            +E +L+RT
Sbjct: 312 FFSLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTGNGLQIEDQPLEVLLNRT 371

Query: 128 PLANISLKIKSDFV 130
           PLAN ++K+KSD+V
Sbjct: 372 PLANGNIKMKSDYV 384

[Close]

BLAST of MC03g_new0281 vs. TAIR 10
Match: AT2G34790.1 (FAD-binding Berberine family protein )

HSP 1 Score: 90.5 bits (223), Expect = 1.1e-18
Identity = 46/132 (34.85%), Postives = 82/132 (62.12%), Query Frame = 0


Query: 8   VPTTVTICSTNRTLEEGAVKLMHRWQYVANKLDENVLLNILWLGGNITGQGGGKINPIAT 67
           VP TVT+ +  +TLE+   K++++W+ +A+KLD+++ + ++    + T + G +   + +
Sbjct: 254 VPATVTVFTVTKTLEQDGTKVLYKWEQIADKLDDDLFIRVIISPASKTTKPGNRTISM-S 313

Query: 68  FSSFFLGKADELLAILKKTFPELHLAKKECTETSWIQSTVLV---------EAMLSRTPL 127
           + + FLG ++ LL +++K+FPEL L KK+CTE SWI+S + +         EA+L+   L
Sbjct: 314 YQAQFLGDSNRLLQVMQKSFPELGLTKKDCTEMSWIKSVMYIAGFPNSAAPEALLAGKSL 373

Query: 128 ANISLKIKSDFV 131
                K KSDFV
Sbjct: 374 FKNHFKAKSDFV 384

[Close]

BLAST of MC03g_new0281 vs. TAIR 10
Match: AT1G30760.1 (FAD-binding Berberine family protein )

HSP 1 Score: 87.0 bits (214), Expect = 1.2e-17
Identity = 50/133 (37.59%), Postives = 79/133 (59.40%), Query Frame = 0


Query: 8   VPTTVTICSTNRTLEEGAVKLMHRWQYVANKLDENVLLNILWLGGNITGQGGGKINPIAT 67
           VP  VT+ +  RTLE+   KL+++WQ VA+KLDE++ + ++    + T +   K   I+T
Sbjct: 256 VPEIVTVFTVTRTLEQDGTKLLYKWQQVADKLDEDLFIRVIIQPTSKTPK--SKERTIST 315

Query: 68  -FSSFFLGKADELLAILKKTFPELHLAKKECTETSWIQSTVLV---------EAMLSRTP 127
            +   FLG A+ LL +++++FP+L L KK+C ETSWI+S + +         EA+L    
Sbjct: 316 SYQGQFLGDANRLLQVMQRSFPQLGLTKKDCLETSWIKSVMYIAGFPSTAPSEALLDGKS 375

Query: 128 LANISLKIKSDFV 131
           L     K KSD+V
Sbjct: 376 LFKNYFKAKSDYV 386

[Close]

BLAST of MC03g_new0281 vs. TAIR 10
Match: AT1G26410.1 (FAD-binding Berberine family protein )

HSP 1 Score: 84.7 bits (208), Expect = 5.9e-17
Identity = 45/132 (34.09%), Postives = 74/132 (56.06%), Query Frame = 0


Query: 8   VPTTVTICSTNRTLEEGAVKLMHRWQYVANKLDENVLLNILWLGGNITGQGGGKINPIAT 67
           VP  +T+   N+TLE+G   ++++WQ VANKL +++ +   W    + G   G+      
Sbjct: 270 VPKILTVFKVNKTLEQGGTDILYKWQLVANKLPDSLFIT-AW-PRTVNGPKPGERTVAVV 329

Query: 68  FSSFFLGKADELLAILKKTFPELHLAKKECTETSWIQSTVL---------VEAMLSRTPL 127
           F + FLG  D+L+ I+ ++FPEL L +++C E SW+ +T+             +L R P 
Sbjct: 330 FYAQFLGPTDKLMEIMDQSFPELGLGREDCHEMSWLNTTLFWANYPAGTPKSILLDRPPT 389

Query: 128 ANISLKIKSDFV 131
            ++S K KSDFV
Sbjct: 390 NSVSFKSKSDFV 399

[Close]

BLAST of MC03g_new0281 vs. TAIR 10
Match: AT5G44400.1 (FAD-binding Berberine family protein )

HSP 1 Score: 83.6 bits (205), Expect = 1.3e-16
Identity = 46/134 (34.33%), Postives = 76/134 (56.72%), Query Frame = 0


Query: 8   VPTTVTICSTNRTLEEG-AVKLMHRWQYVANKLDENVLLNILWLGGNITGQGGGKINPIA 67
           VP TVT+ +  +TL++    K++ +WQ VA+KL E + + +L+   N+ G GG K     
Sbjct: 256 VPKTVTVFTVTKTLQQDVGNKIISKWQRVADKLVEELFIRVLF---NVAGTGGNK-TVTT 315

Query: 68  TFSSFFLGKADELLAILKKTFPELHLAKKECTETSWIQSTVLVEA----------MLSRT 127
           ++++ FLG    L+ ++KK+FPEL L  K+C E SW++S   +            +  ++
Sbjct: 316 SYNALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESIAYISGFPTHTPTNVLLQGKS 375

Query: 128 PLANISLKIKSDFV 131
           P   +S K KSDFV
Sbjct: 376 PFPKVSFKAKSDFV 385

[Close]

BLAST of MC03g_new0281 vs. TAIR 10
Match: AT1G30700.1 (FAD-binding Berberine family protein )

HSP 1 Score: 80.5 bits (197), Expect = 1.1e-15
Identity = 52/133 (39.10%), Postives = 74/133 (55.64%), Query Frame = 0


Query: 8   VPTTVTICSTNRTLEEGAVKLMHRWQYVANKLDENVLLN--ILWLGGNITGQGGGKINPI 67
           VP  VT+   +RTLE+ A  ++HRWQ VA KL + + +   I  + G ++ Q   +    
Sbjct: 247 VPENVTVFRISRTLEQNATDIIHRWQQVAPKLPDELFIRTVIDVVNGTVSSQKTVR---- 306

Query: 68  ATFSSFFLGKADELLAILKKTFPELHLAKKECTETSWIQSTVL---VEAMLSRTPLANIS 127
            TF + FLG    LL+IL + FPEL L + +CTETSWIQS +    ++   S T L   +
Sbjct: 307 TTFIAMFLGDTTTLLSILNRRFPELGLVRSDCTETSWIQSVLFWTNIQVGSSETLLLQRN 366

Query: 128 -----LKIKSDFV 131
                LK KSD+V
Sbjct: 367 QPVNYLKRKSDYV 375

The following BLAST results are available for this feature:

BLAST of MC03g_new0281 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O64743	1.5e-17	34.85	Berberine bridge enzyme-like 15 OS=Arabidopsis thaliana OX=3702 GN=MEE23 PE=1 SV...	[more]
Q93ZA3	1.7e-16	37.59	Berberine bridge enzyme-like 13 OS=Arabidopsis thaliana OX=3702 GN=At1g30760 PE=...	[more]
Q9FZC7	8.3e-16	34.09	Berberine bridge enzyme-like 6 OS=Arabidopsis thaliana OX=3702 GN=FOX4 PE=2 SV=1	[more]
Q9FKU8	1.8e-15	34.33	Berberine bridge enzyme-like 26 OS=Arabidopsis thaliana OX=3702 GN=At5g44400 PE=...	[more]
Q9SA85	1.6e-14	39.10	Berberine bridge enzyme-like 8 OS=Arabidopsis thaliana OX=3702 GN=At1g30700 PE=2...	[more]

BLAST of MC03g_new0281 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022142425.1	1.66e-56	72.93	berberine bridge enzyme-like 18 [Momordica charantia]	[more]
XP_022142587.1	1.27e-53	70.68	tetrahydrocannabinolic acid synthase-like [Momordica charantia]	[more]
XP_022152516.1	1.38e-46	64.39	berberine bridge enzyme-like 18 [Momordica charantia]	[more]
XP_038895343.1	1.63e-42	61.19	cannabidiolic acid synthase-like [Benincasa hispida]	[more]
KAE8653318.1	2.14e-42	58.91	hypothetical protein Csa_023332 [Cucumis sativus]	[more]

BLAST of MC03g_new0281 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CKW8	8.06e-57	72.93	berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111012562 P...	[more]
A0A6J1CN68	6.13e-54	70.68	tetrahydrocannabinolic acid synthase-like OS=Momordica charantia OX=3673 GN=LOC1...	[more]
A0A6J1DHZ2	6.70e-47	64.39	berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020223 P...	[more]
A0A6J1DEU9	1.57e-41	56.82	berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020168 P...	[more]
A0A5A7TYU2	2.16e-41	60.45	Cannabidiolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]

BLAST of MC03g_new0281 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G34790.1	1.1e-18	34.85	FAD-binding Berberine family protein	[more]
AT1G30760.1	1.2e-17	37.59	FAD-binding Berberine family protein	[more]
AT1G26410.1	5.9e-17	34.09	FAD-binding Berberine family protein	[more]
AT5G44400.1	1.3e-16	34.33	FAD-binding Berberine family protein	[more]
AT1G30700.1	1.1e-15	39.10	FAD-binding Berberine family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.462.20		coord: 11..130 e-value: 4.9E-25 score: 90.3
None	No IPR available	PANTHER	PTHR32448:SF113	BERBERINE BRIDGE ENZYME-LIKE 18	coord: 7..130
None	No IPR available	PANTHER	PTHR32448	OS08G0158400 PROTEIN	coord: 7..130

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC03g_new0281.1	MC03g_new0281.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0071949	FAD binding
molecular_function	GO:0016491	oxidoreductase activity