MC03g_new0279 (gene) Bitter gourd (Dali-11) v1

BLAST of MC03g_new0279 vs. ExPASy Swiss-Prot
Match: O04893 (Alpha-glucosidase OS=Spinacia oleracea OX=3562 PE=1 SV=1)

HSP 1 Score: 1071.2 bits (2769), Expect = 6.3e-312
Identity = 524/884 (59.28%), Postives = 662/884 (74.89%), Query Frame = 0

Query: 22  SLVEPQPSPTIGYGYRIRSVHVDP-AGKSLTADLALIKTSSVYGPDLHTLTLQASFETKD 81
           S  E  P   IGYGY+++SV VD    +SLTA   L+K SSVYGPD+  L++ AS E+ D
Sbjct: 26  STSENDPEGVIGYGYKVKSVKVDSGTRRSLTALPQLVKNSSVYGPDIQLLSITASLESND 85

Query: 82  RLRIRITDSTRERWEIPDHII---------PRRSNSRFRSPPETAVDSPATAFISDPASD 141
           RLR+RITD+   RWEIPD+I+         P   +S +R+   +   +     +S P SD
Sbjct: 86  RLRVRITDAKHRRWEIPDNILHRHQPPPPPPHSLSSLYRTLLSSPTTNRRKILLSHPNSD 145

Query: 142 LSFSLRNTTPFGFSVLRRSSGDVLFDTAPDLSDPGTFLVFKDQYIQLSSSLPKDRSSIFG 201
           L+FSL NTTPFGF++ R+S+ DVLFD  PD ++P TFL+F DQY+ L+SSLP  R+ I+G
Sbjct: 146 LTFSLINTTPFGFTISRKSTHDVLFDATPDPTNPNTFLIFIDQYLHLTSSLPGTRAHIYG 205

Query: 202 IGEQIRESFKLVSNKSKTLTLWNGDVGRAVVDVNLYGSHPFYIDVRSPSRDGKVVAGTTH 261
           +GE  + +F+L  N  +TLT+   D+  +  DVNLYGSHPFY+DVRS       VAG+TH
Sbjct: 206 LGEHSKPTFQLAHN--QTLTMRAADIPSSNPDVNLYGSHPFYMDVRS-----SPVAGSTH 265

Query: 262 GVLLLNSNGMDVIYSGDRITYKVIGGVIDLYFFAGPSPVSVIDQYTELIGRPAPVPYWSF 321
           GVLLLNSNGMDV Y+G+RITYKVIGG+IDLYFFAGPSP  V++Q+T +IGRPAP+PYW+F
Sbjct: 266 GVLLLNSNGMDVEYTGNRITYKVIGGIIDLYFFAGPSPGQVVEQFTRVIGRPAPMPYWAF 325

Query: 322 GYHQCRYGYKNVSDVENVVARYAKAGIPLEVVCTDIDYMDEYKDFTFDPINFPVEKMKIF 381
           G+ QCRYGY +V ++++VVA YAKA IPLEV+ TDIDYMD YKDFT DP+NFP++KMK F
Sbjct: 326 GFQQCRYGYHDVYELQSVVAGYAKAKIPLEVMWTDIDYMDAYKDFTLDPVNFPLDKMKKF 385

Query: 382 VDNLHQNGQKYVPILDPGISVNKTYGTFVRGTKADIFIKYDGVPYSGEVWSGPVYFPDFV 441
           V+NLH+NGQKYV ILDPGIS NKTY T++RG K D+F+K +G PY G VW GPVYFPDF+
Sbjct: 386 VNNLHKNGQKYVVILDPGISTNKTYETYIRGMKHDVFLKRNGKPYLGSVWPGPVYFPDFL 445

Query: 442 HPNSEIFWGGEIKLFQDMVSFDGLWIDMNEISNAITSSTSPLSNLDNPPYKINNAAVLRP 501
            P++  FW  EIK F +++  DGLWIDMNEISN I+S   P S LDNPPYKINN+ V+ P
Sbjct: 446 KPSALTFWTDEIKRFLNLLPVDGLWIDMNEISNFISSPPIPGSTLDNPPYKINNSGVMLP 505

Query: 502 INNMTVPASSLHFGNLTEYNTHNLYGLLESRATHASLVKVTGKRPFVLSRSTFVSSGKYT 561
           I N T+P +++H+G++ EYN HNL+G LE+R T A+L+K+T KRPFVLSRSTF  SGKYT
Sbjct: 506 IINKTIPPTAMHYGDIPEYNVHNLFGYLEARVTRAALIKLTEKRPFVLSRSTFSGSGKYT 565

Query: 562 AHWTGDNAATWNHLGYTIPSILNFGLFGIPMVGSDICGFSGDTTEELCRRWIQLGAFYPF 621
           AHWTGDNAATWN L Y+IPS+L+FGLFGIPMVG+DICGF G+TTEELCRRWIQLGAFYPF
Sbjct: 566 AHWTGDNAATWNDLVYSIPSMLDFGLFGIPMVGADICGFLGNTTEELCRRWIQLGAFYPF 625

Query: 622 ARGHSDKLYIRRELYLWDSVAATARKVLALRYRLFPYFYTLMYEAHRKGTPIARPLFFSF 681
           +R HS      +ELY W+SVAA+ARKVL LRY L PYFYTLMYEA   G PIARPLFFSF
Sbjct: 626 SRDHSSLGTTYQELYRWESVAASARKVLGLRYTLLPYFYTLMYEAQLNGIPIARPLFFSF 685

Query: 682 PQDVETYEISSQFLLGQGVLISPVLMEGAVSVDAYFPAGNWFSLFNYSESVSVKSGQQVT 741
           P D++TY ISSQFLLG+GV++SPVL  G VSV AYFP GNWF LF+Y+ SV+  +G+ VT
Sbjct: 686 PDDIKTYGISSQFLLGKGVMVSPVLKPGVVSVTAYFPRGNWFDLFDYTRSVTASTGRYVT 745

Query: 742 LDAPADHINVHVREGNILALHGDAMTTRAARETAFKLLVVVSNGRSSNGEVFLDDGEVVE 801
           L AP DHINVH++EGNILA+ G AMTT+AAR+T F LLVV+S+  +S GE+FLDDG  V 
Sbjct: 746 LSAPPDHINVHIQEGNILAMQGKAMTTQAARKTPFHLLVVMSDCGASFGELFLDDGVEVT 805

Query: 802 MGEEGGNWSLVRFYSEAVGNKLLIKSQVINGGFALSQKLIIDKVTFVGFEWPKKMGGFGL 861
           MG   G W+ V+F + +     +I S V++G FA+SQK +IDKVT +G     K+ G+ +
Sbjct: 806 MGVNRGKWTFVKFIAASAKQTCIITSDVVSGEFAVSQKWVIDKVTILGLRKGTKINGYTV 865

Query: 862 KISKGANLNGNSGIRKTYEYSGKFVNVEISALSIPIWEEFKLEL 896
           +          S ++ T +  G+F+  EIS L++ +  EFKL L
Sbjct: 866 RTGAVTRKGDKSKLKSTPDRKGEFIVAEISGLNLLLGREFKLVL 902

BLAST of MC03g_new0279 vs. ExPASy Swiss-Prot
Match: O04931 (Alpha-glucosidase OS=Beta vulgaris OX=161934 PE=1 SV=1)

HSP 1 Score: 1036.9 bits (2680), Expect = 1.3e-301
Identity = 499/875 (57.03%), Postives = 656/875 (74.97%), Query Frame = 0

Query: 32  IGYGYRIRSVHVD-PAGKSLTADLALIKTSSVYGPDLHTLTLQASFETKDRLRIRITDST 91
           IGYGY++++  VD   GKSLTA L LI+ S VYGPD+H L+  ASFE  D LRIR TD+ 
Sbjct: 41  IGYGYQVKNAKVDNSTGKSLTALLQLIRNSPVYGPDIHFLSFTASFEEDDTLRIRFTDAN 100

Query: 92  RERWEIPDHIIPRRS--------NSRFRSPPETAVDSPATAFISDPASDLSFSLRNTTPF 151
             RWEIP+ ++PR          +S    P     + P T  +S P SDL+F+L +TTPF
Sbjct: 101 NRRWEIPNEVLPRPPPPPSPPPLSSLQHLPKPIPQNQPTTTVLSHPHSDLAFTLFHTTPF 160

Query: 152 GFSVLRRSSGDVLFDTAPDLSDPGTFLVFKDQYIQLSSSLPKDRSSIFGIGEQIRESFKL 211
           GF++ R+S+ DVLFD  P  S+P TFL++KDQY+QLSSSLP  ++ ++G+GE  + +F+L
Sbjct: 161 GFTIYRKSTHDVLFDATPIPSNPTTFLIYKDQYLQLSSSLPAQQAHLYGLGEHTKPTFQL 220

Query: 212 VSNKSKTLTLWNGDVGRAVVDVNLYGSHPFYIDVRSPSRDGKVVAGTTHGVLLLNSNGMD 271
             N  + LTLWN D+     D+NLYGSHPFY+DVRS       + G+THGV LLNSNGMD
Sbjct: 221 AHN--QILTLWNADIASFNRDLNLYGSHPFYMDVRS-----SPMVGSTHGVFLLNSNGMD 280

Query: 272 VIYSGDRITYKVIGGVIDLYFFAGPSPVSVIDQYTELIGRPAPVPYWSFGYHQCRYGYKN 331
           V Y+GDRITYKVIGG+IDLY FAG +P  V+DQYT+LIGRPAP+PYW+FG+HQCR+GY++
Sbjct: 281 VEYTGDRITYKVIGGIIDLYIFAGRTPEMVLDQYTKLIGRPAPMPYWAFGFHQCRWGYRD 340

Query: 332 VSDVENVVARYAKAGIPLEVVCTDIDYMDEYKDFTFDPINFPVEKMKIFVDNLHQNGQKY 391
           V+++E VV +YA+A IPLEV+ TDIDYMD +KDFT DP++FP++KM+ FV  LH+NGQ+Y
Sbjct: 341 VNEIETVVDKYAEARIPLEVMWTDIDYMDAFKDFTLDPVHFPLDKMQQFVTKLHRNGQRY 400

Query: 392 VPILDPGISVNKTYGTFVRGTKADIFIKYDGVPYSGEVWSGPVYFPDFVHPNSEIFWGGE 451
           VPILDPGI+ NK+YGTF+RG ++++FIK +G PY G VW GPVY+PDF+ P +  FW  E
Sbjct: 401 VPILDPGINTNKSYGTFIRGMQSNVFIKRNGNPYLGSVWPGPVYYPDFLDPAARSFWVDE 460

Query: 452 IKLFQDMVSFDGLWIDMNEISNAITSSTSPLSNLDNPPYKINNAAVLRPINNMTVPASSL 511
           IK F+D++  DG+WIDMNE SN ITS+ +P S LDNPPYKINN+    PIN+ T+PA+++
Sbjct: 461 IKRFRDILPIDGIWIDMNEASNFITSAPTPGSTLDNPPYKINNSGGRVPINSKTIPATAM 520

Query: 512 HFGNLTEYNTHNLYGLLESRATHASLVKVTGKRPFVLSRSTFVSSGKYTAHWTGDNAATW 571
           H+GN+TEYN HNLYG LES+AT  +LV+   + PF+LSRSTF  SGKYTAHWTGDNAA W
Sbjct: 521 HYGNVTEYNAHNLYGFLESQATREALVRPATRGPFLLSRSTFAGSGKYTAHWTGDNAARW 580

Query: 572 NHLGYTIPSILNFGLFGIPMVGSDICGFSGDTTEELCRRWIQLGAFYPFARGHSDKLYIR 631
           + L Y+IP++LNFGLFG+PM+G+DICGF+  TTEELC RWIQLGAFYPF+R HS +    
Sbjct: 581 DDLQYSIPTMLNFGLFGMPMIGADICGFAESTTEELCCRWIQLGAFYPFSRDHSARDTTH 640

Query: 632 RELYLWDSVAATARKVLALRYRLFPYFYTLMYEAHRKGTPIARPLFFSFPQDVETYEISS 691
           +ELYLW+SVAA+AR VL LRY L PY+YTLMY+A+ +G+PIARPL F+FP DV TY ISS
Sbjct: 641 QELYLWESVAASARTVLGLRYELLPYYYTLMYDANLRGSPIARPLSFTFPDDVATYGISS 700

Query: 692 QFLLGQGVLISPVLMEGAVSVDAYFPAGNWFSLFNYSESVSVKSGQQVTLDAPADHINVH 751
           QFL+G+G+++SPVL  G+  V+AY P GNW SL NY+ SVSV +G  V+L AP DHINVH
Sbjct: 701 QFLIGRGIMVSPVLQPGSSIVNAYSPRGNWVSLSNYTSSVSVSAGTYVSLSAPPDHINVH 760

Query: 752 VREGNILALHGDAMTTRAARETAFKLLVVVSNGRSSNGEVFLDDGEVVEMGEEGGNWSLV 811
           + EGNI+A+ G+AMTT+AAR T F LLVV+S+  +S GE+FLD+G  +++G  GG W+LV
Sbjct: 761 IHEGNIVAMQGEAMTTQAARSTPFHLLVVMSDHVASTGELFLDNGIEMDIGGPGGKWTLV 820

Query: 812 RFYSEAVGNKLLIKSQVINGGFALSQKLIIDKVTFVGFEWPKKMGGFGLKISKGANLNGN 871
           RF++E+  N L I S+V+N G+A+SQ+ ++DK+T +G +   K+  + ++   GA     
Sbjct: 821 RFFAESGINNLTISSEVVNRGYAMSQRWVMDKITILGLKRRVKIKEYTVQKDAGAIKVKG 880

Query: 872 SGIRKTYEYSGKFVNVEISALSIPIWEEFKLELTF 898
            G R +    G F    IS L   + + FKLEL F
Sbjct: 881 LGRRTSSHNQGGFFVSVISDLRQLVGQAFKLELEF 908

BLAST of MC03g_new0279 vs. ExPASy Swiss-Prot
Match: Q653V7 (Probable alpha-glucosidase Os06g0675700 OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0675700 PE=1 SV=1)

HSP 1 Score: 958.7 bits (2477), Expect = 4.5e-278
Identity = 472/840 (56.19%), Postives = 612/840 (72.86%), Query Frame = 0

Query: 64  GPDLHTLTLQASFETKDRLRIRITDSTRERWEIPDHIIPRRSNSRFRSPPETAVDSPATA 123
           GPD+  L+L AS ET  RL +RITD+   RWE+P  +IPR S   F +    A       
Sbjct: 65  GPDVRRLSLTASLETDSRLHVRITDADHPRWEVPQDVIPRPSPDSFLA----ATRPGGGR 124

Query: 124 FISDPASDLSFSLRNTTPFGFSVLRRSSGDVLFDTAPDLSDPGTFLVFKDQYIQLSSSL- 183
            +S   SDL+F++ +T+PF F+V RRS+GDVLFDT P+       LVFKD+Y++L+SSL 
Sbjct: 125 VLSTATSDLTFAI-HTSPFRFTVTRRSTGDVLFDTTPN-------LVFKDRYLELTSSLP 184

Query: 184 PKDRSSIFGIGEQIRESFKLVSNKSKTLTLWNGDVGRAVVDVNLYGSHPFYIDVRSPSRD 243
           P  R+S++G+GEQ + +F+L   ++ T TLWN D+    VD+NLYGSHPFY+DVRS    
Sbjct: 185 PPGRASLYGLGEQTKRTFRL--QRNDTFTLWNSDIAAGNVDLNLYGSHPFYMDVRSGGGG 244

Query: 244 GKVVAGTTHGVLLLNSNGMDVIYSGDRITYKVIGGVIDLYFFAGPSPVSVIDQYTELIGR 303
           G    G  HGVLLLNSNGMDVIY G  +TYKVIGGV+D YFFAGPSP++V+DQYT+LIGR
Sbjct: 245 G---GGAAHGVLLLNSNGMDVIYGGSYVTYKVIGGVLDFYFFAGPSPLAVVDQYTQLIGR 304

Query: 304 PAPVPYWSFGYHQCRYGYKNVSDVENVVARYAKAGIPLEVVCTDIDYMDEYKDFTFDPIN 363
           PAP+PYWSFG+HQCRYGYKNV+D+E VVA YAKA IPLEV+ TDIDYMD YKDFT DP+N
Sbjct: 305 PAPMPYWSFGFHQCRYGYKNVADLEGVVAGYAKARIPLEVMWTDIDYMDAYKDFTLDPVN 364

Query: 364 FPVEKMKIFVDNLHQNGQKYVPILDPGISVNKTYGTFVRGTKADIFIKYDGVPYSGEVWS 423
           FP ++M+ FVD LH+NGQK+V I+DPGI+VN TYGTFVRG K DIF+K++G  Y G VW 
Sbjct: 365 FPADRMRPFVDRLHRNGQKFVVIIDPGINVNTTYGTFVRGMKQDIFLKWNGSNYLGVVWP 424

Query: 424 GPVYFPDFVHPNSEIFWGGEIKLFQDMVSFDGLWIDMNEISNAITSSTSPLSNLDNPPYK 483
           G VYFPDF++P +  FW  EI  F+  +  DGLW+DMNEISN +     PL+ +D+PPY+
Sbjct: 425 GNVYFPDFLNPRAAEFWAREIAAFRRTLPVDGLWVDMNEISNFV--DPPPLNAIDDPPYR 484

Query: 484 INNAAVLRPINNMTVPASSLHFGNLTEYNTHNLYGLLESRATHASLVKVTGKRPFVLSRS 543
           INN+ V RPINN TVPAS++H+G + EY+ HNL+G LE+RATH +L++ TG+RPFVLSRS
Sbjct: 485 INNSGVRRPINNKTVPASAVHYGGVAEYDAHNLFGFLEARATHDALLRDTGRRPFVLSRS 544

Query: 544 TFVSSGKYTAHWTGDNAATWNHLGYTIPSILNFGLFGIPMVGSDICGFSGDTTEELCRRW 603
           TFV SG+YTAHWTGDNAATW  L Y+I ++L+FGLFGIPM+G+DICGF G+TTEELC RW
Sbjct: 545 TFVGSGRYTAHWTGDNAATWEDLHYSINTMLSFGLFGIPMIGADICGFGGNTTEELCSRW 604

Query: 604 IQLGAFYPFARGHSDKLYIRRELYLWDSVAATARKVLALRYRLFPYFYTLMYEAHRKGTP 663
           IQLGAFYPF+R HS    +RRELYLW+SVA +ARK L LRYRL PY YTLMYEAH  G P
Sbjct: 605 IQLGAFYPFSRDHSAIGTVRRELYLWESVARSARKALGLRYRLLPYLYTLMYEAHTTGAP 664

Query: 664 IARPLFFSFPQDVETYEISSQFLLGQGVLISPVLMEGAVSVDAYFPAGNWFSLFNYSESV 723
           IARPLFFS+P DVETY I  QFLLG+GVL+SPVL  GA +V AYFPAG WFSL+++S +V
Sbjct: 665 IARPLFFSYPGDVETYGIDRQFLLGRGVLVSPVLEPGATTVTAYFPAGRWFSLYDFSLAV 724

Query: 724 SVKSGQQVTLDAPADHINVHVREGNILALHGDAMTTRAARETAFKLLVVVSNGRSSNGEV 783
           + K+G++VTL APAD +NVHV  GNIL L   A+T+   R++   LLV +++  ++ G++
Sbjct: 725 ATKTGKRVTLPAPADTVNVHVAGGNILTLQQPALTSSRVRQSVVHLLVALADDGTATGDL 784

Query: 784 FLDDGEVVEMGEEGGNWSLVRF--YSEAVGNKLLIKSQVINGGFALSQKLIIDKVTFVGF 843
           FLDDGE  EM      WS ++F   +E+ G  + ++S V++  +A S+ + I KV  +G 
Sbjct: 785 FLDDGESPEMAGPRSRWSQIKFSGATESGGGVVRVRSHVVHDSYAPSRTMAIGKVVLMGL 844

Query: 844 EWPKKMGGF-----GLKISKGANLNGNSGIRKTYEYSGKFVNVEISALSIPIWEEFKLEL 896
                  GF     G++++    + G +G  +     G      +S L++ + +EF L++
Sbjct: 845 RSAAPPKGFAVYANGVQVNASTAVGGAAGSPE----KGALGVAHVSGLTLVVGQEFDLKV 881

BLAST of MC03g_new0279 vs. ExPASy Swiss-Prot
Match: Q43763 (Alpha-glucosidase OS=Hordeum vulgare OX=4513 PE=2 SV=1)

HSP 1 Score: 920.2 bits (2377), Expect = 1.8e-266
Identity = 460/837 (54.96%), Postives = 596/837 (71.21%), Query Frame = 0

Query: 66  DLHTLTLQASFETKDRLRIRITDSTRERWEIPDHIIPRRSNSRFRSPPETAVDSPATAFI 125
           D+  L + AS ET  RLR+RITD+   RWE+P  IIPR +          A  +P    +
Sbjct: 59  DVQRLAVYASLETDSRLRVRITDADHPRWEVPQDIIPRPAPGDVLHDAPPASSAPLQGRV 118

Query: 126 SDPA-SDLSFSLRNTTPFGFSVLRRSSGDVLFDTAPDLSDPGTFLVFKDQYIQLSSSLPK 185
             PA SDL  ++ + +PF F+V RRS+GD LFDTAP        LVF+D+Y++++S+LP 
Sbjct: 119 LSPAGSDLVLTV-HASPFRFTVSRRSTGDTLFDTAPG-------LVFRDKYLEVTSALPA 178

Query: 186 DRSSIFGIGEQIRESFKLVSNKSKTLTLWNGDVGRAVVDVNLYGSHPFYIDVRSPSRDGK 245
            R+S++G+GE  + SF+L  N S   TLWN D+G + VDVNLYGSHPFY+DVR+P     
Sbjct: 179 GRASLYGLGEHTKSSFRLRHNDS--FTLWNADIGASYVDVNLYGSHPFYMDVRAP----- 238

Query: 246 VVAGTTHGVLLLNSNGMDVIYSGDRITYKVIGGVIDLYFFAGPSPVSVIDQYTELIGRPA 305
              GT HGVLLL+SNGMDV+Y G  +TYKVIGGV+D YFFAGP+P++V+DQYT+LI RPA
Sbjct: 239 ---GTAHGVLLLSSNGMDVLYGGSYVTYKVIGGVLDFYFFAGPNPLAVVDQYTQLIARPA 298

Query: 306 PVPYWSFGYHQCRYGYKNVSDVENVVARYAKAGIPLEVVCTDIDYMDEYKDFTFDPINFP 365
           P+PYWSFG+HQCRYGY NVSD+E VVARYAKA IPLEV+ TDIDYMD +KDFT D +NF 
Sbjct: 299 PMPYWSFGFHQCRYGYLNVSDLERVVARYAKARIPLEVMWTDIDYMDGFKDFTLDRVNFT 358

Query: 366 VEKMKIFVDNLHQNGQKYVPILDPGISV---NKTYGTFVRGTKADIFIKYDGVPYSGEVW 425
             +++ FVD LH+N QKYV ILDPGI V   + TYGTFVRG + DIF+K +G  + G VW
Sbjct: 359 AAELRPFVDRLHRNAQKYVLILDPGIRVDPIDATYGTFVRGMQQDIFLKRNGTNFVGNVW 418

Query: 426 SGPVYFPDFVHPNSEIFWGGEIKLFQDMVSFDGLWIDMNEISNAITSSTSPLSNLDNPPY 485
            G VYFPDF+HP +  FW  EI LF+  +  DGLWIDMNEISN    +  P++ LD+PPY
Sbjct: 419 PGDVYFPDFMHPAAAEFWAREISLFRRTIPVDGLWIDMNEISNFY--NPEPMNALDDPPY 478

Query: 486 KINNAAVLRPINNMTVPASSLHFGNLTEYNTHNLYGLLESRATHASLVKVTGKRPFVLSR 545
           +INN    RPINN TV   ++H+G +TEY  HNL+GLLE+RAT   +++ TG+RPFVLSR
Sbjct: 479 RINNDGTGRPINNKTVRPLAVHYGGVTEYEEHNLFGLLEARATGRGVLRDTGRRPFVLSR 538

Query: 546 STFVSSGKYTAHWTGDNAATWNHLGYTIPSILNFGLFGIPMVGSDICGFSGDTTEELCRR 605
           STFV SG+YTA+WTGDNAATW  L Y+I ++L+FGLFG+PM+G+DICGF+G+TTEELC R
Sbjct: 539 STFVGSGRYTAYWTGDNAATWGDLRYSINTMLSFGLFGMPMIGADICGFNGNTTEELCGR 598

Query: 606 WIQLGAFYPFARGHSDKLYIRRELYLWDSVAATARKVLALRYRLFPYFYTLMYEAHRKGT 665
           WIQLGAFYPF+R HS    +RRELYLW SVAA+ RK L LRY+L PYFYTLMYEAH  G 
Sbjct: 599 WIQLGAFYPFSRDHSAIFTVRRELYLWPSVAASGRKALGLRYQLLPYFYTLMYEAHMTGA 658

Query: 666 PIARPLFFSFPQDVETYEISSQFLLGQGVLISPVLMEGAVSVDAYFPAGNWFSLFNYSES 725
           PIARPLFFS+P DV TY +  QFLLG+GVL+SPVL  G  +VDAYFPAG W+ L++YS +
Sbjct: 659 PIARPLFFSYPHDVATYGVDRQFLLGRGVLVSPVLEPGPTTVDAYFPAGRWYRLYDYSLA 718

Query: 726 VSVKSGQQVTLDAPADHINVHVREGNILALHGDAMTTRAARETAFKLLVVVSNGRSSNGE 785
           V+ ++G+ V L APAD +NVH+  G IL L   A+TT  AR TAF LLV ++   +++G 
Sbjct: 719 VATRTGKHVRLPAPADTVNVHLTGGTILPLQQSALTTSRARRTAFHLLVALAEDGTASGY 778

Query: 786 VFLDDGEVVEMGEEGGNWSLVRFYSEAVGNK--LLIKSQVINGGFALSQKLIIDKVTFVG 845
           +FLDDG+  E G    +WS+VRF  +   NK  + +KS+V++  +A S+ L+I KV  +G
Sbjct: 779 LFLDDGDSPEYGRR-SDWSMVRFNYKIPNNKGAIKVKSEVVHNSYAQSRTLVISKVVLMG 838

Query: 846 FEWPKKMGGFGLKISKGANLNGNSGIRKTYEYSGKFVNV-EISALSIPIWEEFKLEL 896
              P       + ++  A +  +S     Y+ +G    V  I  LS+ + EEF+L++
Sbjct: 839 HRSPAAPKKLTVHVN-SAEVEASSSAGTRYQNAGGLGGVAHIGGLSLVVGEEFELKV 873

BLAST of MC03g_new0279 vs. ExPASy Swiss-Prot
Match: Q9S7Y7 (Alpha-xylosidase 1 OS=Arabidopsis thaliana OX=3702 GN=XYL1 PE=1 SV=1)

HSP 1 Score: 781.6 bits (2017), Expect = 9.8e-225
Identity = 414/930 (44.52%), Postives = 572/930 (61.51%), Query Frame = 0

Query: 1   MAKTATICIFGFPL---FIICFFFSLVEPQPSPTIGYGYRIRSVHVDPAGKSLTADLALI 60
           MA +++   F   L    I+CF       Q   TIG GYR+ S+   P G      L + 
Sbjct: 1   MASSSSSLAFSLSLLLALILCF----SPTQSYKTIGKGYRLVSIEESPDG-GFIGYLQVK 60

Query: 61  KTSSVYGPDLHTLTLQASFETKDRLRIRITDSTRERWEIPDHIIPRRSNSRFRSPPETAV 120
           + + +YG D+ TL L    ET  RLR+ ITD+ ++RWE+P +++PR    +       + 
Sbjct: 61  QKNKIYGSDITTLRLFVKHETDSRLRVHITDAKQQRWEVPYNLLPREQPPQVGKVIGKSR 120

Query: 121 DSPATAFISDPASDLSFSLRNTTPFGFSVLRRSSGDVLFDTAPDLSDPGTFLVFKDQYIQ 180
            SP T       S+L FS   T PF F+V RRS+ + LF+T        + LVFKDQY++
Sbjct: 121 KSPITV-QEISGSELIFSY-TTDPFTFAVKRRSNHETLFNTT-------SSLVFKDQYLE 180

Query: 181 LSSSLPKDRSSIFGIGEQIR-ESFKLVSNKSKTLTLWNGDVGRAVVDVNLYGSHPFYIDV 240
           +S+SLPK+ +S++G+GE  +    KLV N  +  TL+  DV    ++ +LYGSHP Y+D+
Sbjct: 181 ISTSLPKE-ASLYGLGENSQANGIKLVPN--EPYTLYTEDVSAINLNTDLYGSHPMYMDL 240

Query: 241 RSPSRDGKVVAGTTHGVLLLNSNGMDVIYSGDRITYKVIGGVIDLYFFAGPSPVSVIDQY 300
           R+    GK  A   H VLLLNSNGMDV Y GD +TYKVIGGV D YF AGPSP++V+DQY
Sbjct: 241 RNVG--GKAYA---HAVLLLNSNGMDVFYRGDSLTYKVIGGVFDFYFIAGPSPLNVVDQY 300

Query: 301 TELIGRPAPVPYWSFGYHQCRYGYKNVSDVENVVARYAKAGIPLEVVCTDIDYMDEYKDF 360
           T+LIGRPAP+PYWS G+HQCR+GY N+S VE+VV  Y KA IPL+V+  D D+MD +KDF
Sbjct: 301 TQLIGRPAPMPYWSLGFHQCRWGYHNLSVVEDVVDNYKKAKIPLDVIWNDDDHMDGHKDF 360

Query: 361 TFDPINFPVEKMKIFVDNLHQNGQKYVPILDPGISVNKTYGTFVRGTKADIFIKYDGVPY 420
           T +P+ +P  K+  F+D +H+ G KY+ I DPGI VN +YGTF R   AD+FIKY+G P+
Sbjct: 361 TLNPVAYPRAKLLAFLDKIHKIGMKYIVINDPGIGVNASYGTFQRAMAADVFIKYEGKPF 420

Query: 421 SGEVWSGPVYFPDFVHPNSEIFWGGEIKLFQDMVSFDGLWIDMNEISN------------ 480
             +VW GPVYFPDF++P +  +WG EIK F D+V  DGLWIDMNE+SN            
Sbjct: 421 LAQVWPGPVYFPDFLNPKTVSWWGDEIKRFHDLVPIDGLWIDMNEVSNFCSGLCTIPEGK 480

Query: 481 ------------AITSSTSPLSNLDNPPYKINNAAVLRPINNMTVPASSLHFGNLTEYNT 540
                        +       +  D+PPYKIN   V+ P+   T+  S+ H+  + EY+ 
Sbjct: 481 QCPSGEGPGWVCCLDCKNITKTRWDDPPYKINATGVVAPVGFKTIATSATHYNGVREYDA 540

Query: 541 HNLYGLLESRATHASLVKVTGKRPFVLSRSTFVSSGKYTAHWTGDNAATWNHLGYTIPSI 600
           H++YG  E+ ATH  L+ V GKRPF+LSRSTFV SG+Y AHWTGDN  TW  L  +I ++
Sbjct: 541 HSIYGFSETIATHKGLLNVQGKRPFILSRSTFVGSGQYAAHWTGDNQGTWQSLQVSISTM 600

Query: 601 LNFGLFGIPMVGSDICGFSGDTTEELCRRWIQLGAFYPFARGHSDKLYIRRELYLWDSVA 660
           LNFG+FG+PMVGSDICGF    TEELC RWI++GAFYPF+R H++    R+ELY WD+VA
Sbjct: 601 LNFGIFGVPMVGSDICGFYPQPTEELCNRWIEVGAFYPFSRDHANYYSPRQELYQWDTVA 660

Query: 661 ATARKVLALRYRLFPYFYTLMYEAHRKGTPIARPLFFSFPQDVETYEISSQFLLGQGVLI 720
            +AR  L +RY++ P+ YTL YEAH  G PIARPLFFSFP+  E Y  S QFLLG   +I
Sbjct: 661 DSARNALGMRYKILPFLYTLNYEAHMTGAPIARPLFFSFPEYTECYGNSRQFLLGSSFMI 720

Query: 721 SPVLMEGAVSVDAYFPAGNWFSLFNYSESVSVKSGQQVTLDAPADHINVHVREGNILALH 780
           SPVL +G   V+A FP G+W+ +F+ +++V  K+G++VTL AP + +NVH+ +  IL   
Sbjct: 721 SPVLEQGKTEVEALFPPGSWYHMFDMTQAVVSKNGKRVTLPAPLNFVNVHLYQNTILPTQ 780

Query: 781 GDAMTTRAARETAFKLLVVVSNGRS---SNGEVFLDDGEVVEMGEEGGNWSLVRFYSEAV 840
              + ++ AR T F L++    G S   + G+++LD+ E+ EM    G  + V FY+   
Sbjct: 781 QGGLISKDARTTPFSLVIAFPAGASEGYATGKLYLDEDELPEMKLGNGQSTYVDFYASVG 840

Query: 841 GNKLLIKSQVINGGFALSQKLIIDKVTFVGFEWPKKMGGFGLKISKGANLNGNSGIRKTY 894
              + + SQV  G FALS+  +I+KV+ +G     ++    +  S        S    TY
Sbjct: 841 NGTMKMWSQVKEGKFALSKGWVIEKVSVLGLRGAGQVSEIQINGSPMTKKIEVSSKEHTY 900

BLAST of MC03g_new0279 vs. NCBI nr
Match: XP_022142682.1 (alpha-glucosidase-like [Momordica charantia])

HSP 1 Score: 1652 bits (4277), Expect = 0.0
Identity = 818/900 (90.89%), Postives = 852/900 (94.67%), Query Frame = 0

Query: 1   MAKTATICIFGFPLFI-ICFFF-SLVEPQPSPTIGYGYRIRSVHVDPAGKSLTADLALIK 60
           M +TAT    G   FI ICFFF  L +P P P  G+GYRIRSV + P GKSLTADLALI 
Sbjct: 27  MQRTATRATSGLSFFILICFFFLCLAQPPPPPPAGHGYRIRSVQLHPTGKSLTADLALIG 86

Query: 61  TSS-VYGPDLHTLTLQASFETKDRLRIRITDSTRERWEIPDHIIPRRSNSRFRSPPETAV 120
           TSS VYGPDL TLTLQASFETKDRLRIRITDS RERWEIPD+IIPRRSNSRFRS PE+ V
Sbjct: 87  TSSSVYGPDLPTLTLQASFETKDRLRIRITDSARERWEIPDNIIPRRSNSRFRSLPESHV 146

Query: 121 DSPATAFISDPASDLSFSLRNTTPFGFSVLRRSSGDVLFDTAPDLSDPGTFLVFKDQYIQ 180
           DSPATAFISDPASDL+FSLRNTTPFGFSVLRRSSGDVLFDTAPDLSDPGTFLVFKDQYIQ
Sbjct: 147 DSPATAFISDPASDLAFSLRNTTPFGFSVLRRSSGDVLFDTAPDLSDPGTFLVFKDQYIQ 206

Query: 181 LSSSLPKDRSSIFGIGEQIRESFKLVSNKSKTLTLWNGDVGRAVVDVNLYGSHPFYIDVR 240
           LSS LP DRSS+FGIGEQ RESFKLVSNKSKTLTLWN D+GRA+VDVNLYGSHPFYIDVR
Sbjct: 207 LSSLLPNDRSSLFGIGEQTRESFKLVSNKSKTLTLWNADIGRAIVDVNLYGSHPFYIDVR 266

Query: 241 SPSRDGKVVAGTTHGVLLLNSNGMDVIYSGDRITYKVIGGVIDLYFFAGPSPVSVIDQYT 300
           SPSRDGKVVAGTTHGVLLLNSNGMD+IYSGDRITYKVIGGVIDLYFFAGPSPVSVIDQYT
Sbjct: 267 SPSRDGKVVAGTTHGVLLLNSNGMDIIYSGDRITYKVIGGVIDLYFFAGPSPVSVIDQYT 326

Query: 301 ELIGRPAPVPYWSFGYHQCRYGYKNVSDVENVVARYAKAGIPLEVVCTDIDYMDEYKDFT 360
           ELIGRPAPVPYWSFG+HQCRYGYKNVSDVENVVARYAKAGIPLEV+ TDIDYMD YKDFT
Sbjct: 327 ELIGRPAPVPYWSFGFHQCRYGYKNVSDVENVVARYAKAGIPLEVMWTDIDYMDGYKDFT 386

Query: 361 FDPINFPVEKMKIFVDNLHQNGQKYVPILDPGISVNKTYGTFVRGTKADIFIKYDGVPYS 420
           FDPINFP EKMKIFVDNLHQNGQKYV ILDPGIS+NKTYGTF+RGTKADIFIKY+GVPYS
Sbjct: 387 FDPINFPAEKMKIFVDNLHQNGQKYVAILDPGISINKTYGTFIRGTKADIFIKYNGVPYS 446

Query: 421 GEVWSGPVYFPDFVHPNSEIFWGGEIKLFQDMVSFDGLWIDMNEISNAITSSTSPLSNLD 480
           GEVWSGPVYFPDFVHPNSEIFWGGEIKLF+D+V FDGLWIDMNEISNAITSSTSPLSNLD
Sbjct: 447 GEVWSGPVYFPDFVHPNSEIFWGGEIKLFRDIVPFDGLWIDMNEISNAITSSTSPLSNLD 506

Query: 481 NPPYKINNAAVLRPINNMTVPASSLHFGNLTEYNTHNLYGLLESRATHASLVKVTGKRPF 540
           NPPYKINNAAVLRPINN TVPASSLHFGNLTEYNTHNLYGLLESRATHASLVKVTGKRPF
Sbjct: 507 NPPYKINNAAVLRPINNNTVPASSLHFGNLTEYNTHNLYGLLESRATHASLVKVTGKRPF 566

Query: 541 VLSRSTFVSSGKYTAHWTGDNAATWNHLGYTIPSILNFGLFGIPMVGSDICGFSGDTTEE 600
           V+SRSTFV SGKYTAHWTGDN ATWN LGYTIPSILNFGLFGIPMVGSDICGF+GDTTEE
Sbjct: 567 VVSRSTFVGSGKYTAHWTGDNDATWNDLGYTIPSILNFGLFGIPMVGSDICGFAGDTTEE 626

Query: 601 LCRRWIQLGAFYPFARGHSDKLYIRRELYLWDSVAATARKVLALRYRLFPYFYTLMYEAH 660
           LCRRWIQLGAFYPFARGHSDK YIRRELYLWDSVAATARKVLALRYRL PYFYTLMYEAH
Sbjct: 627 LCRRWIQLGAFYPFARGHSDKFYIRRELYLWDSVAATARKVLALRYRLLPYFYTLMYEAH 686

Query: 661 RKGTPIARPLFFSFPQDVETYEISSQFLLGQGVLISPVLMEGAVSVDAYFPAGNWFSLFN 720
           RKGTPIARPLFFSFPQD++TYEISSQFLLG+GVLISPVL EGA+SVDAYFP GNWFSLFN
Sbjct: 687 RKGTPIARPLFFSFPQDIKTYEISSQFLLGKGVLISPVLKEGALSVDAYFPEGNWFSLFN 746

Query: 721 YSESVSVKSGQQVTLDAPADHINVHVREGNILALHGDAMTTRAARETAFKLLVVVSNGRS 780
           YSESVSVKSGQQVTLDAPADHINVHVREGNIL LHGDAMTTRAAR TAFKLLVVVSNG+S
Sbjct: 747 YSESVSVKSGQQVTLDAPADHINVHVREGNILVLHGDAMTTRAARGTAFKLLVVVSNGQS 806

Query: 781 SNGEVFLDDGEVVEMGEEGGNWSLVRFYSEAVGNKLLIKSQVINGGFALSQKLIIDKVTF 840
           S+GEVFLDDG VVEMGEEGGNWSLVRF+SEAVG+KLLIKS+V+NGGFAL+QKLIIDKVTF
Sbjct: 807 SDGEVFLDDGGVVEMGEEGGNWSLVRFHSEAVGSKLLIKSEVVNGGFALTQKLIIDKVTF 866

Query: 841 VGFEWPKKMGGFGLKISKGANLNGNSGIRKTYEYSGKFVNVEISALSIPIWEEFKLELTF 897
           +GFE  +KMGGFGLKISKGANLNGNS I+KTYEYSGKFVNVEISALSIPIWEEFKLELTF
Sbjct: 867 IGFERHEKMGGFGLKISKGANLNGNSAIKKTYEYSGKFVNVEISALSIPIWEEFKLELTF 926

BLAST of MC03g_new0279 vs. NCBI nr
Match: XP_023001112.1 (alpha-glucosidase [Cucurbita maxima])

HSP 1 Score: 1531 bits (3965), Expect = 0.0
Identity = 749/891 (84.06%), Postives = 809/891 (90.80%), Query Frame = 0

Query: 14  LFIICFFFSLV--------EPQPSPTIGYGYRIRSVHVDPAGKSLTADLALIKTSSVYGP 73
           LF+   FF L+        E + +  +GYGYR+RS  VDP GKSLTADL LI+ SSVYGP
Sbjct: 44  LFLFSCFFRLIASLPFAAGEQRENSAVGYGYRVRSGRVDPTGKSLTADLDLIEKSSVYGP 103

Query: 74  DLHTLTLQASFETKDRLRIRITDSTRERWEIPDHIIPRRSNSRFRSPPETAVDSPATAFI 133
           D+  L+LQASFETKDRLR+RITDS RERWEIPD IIPRRSNSR RS PE  V SP T+ I
Sbjct: 104 DVERLSLQASFETKDRLRVRITDSNRERWEIPDEIIPRRSNSRIRSLPERDVGSPETSII 163

Query: 134 SDPASDLSFSLRNTTPFGFSVLRRSSGDVLFDTAPDLSDPGTFLVFKDQYIQLSSSLPKD 193
           SDPASDL FSL +T PFGFSV RRSSGDVLFDT+P+ SD  TFLVFKDQYIQLSSSLPKD
Sbjct: 164 SDPASDLIFSLHDTAPFGFSVHRRSSGDVLFDTSPNFSDSETFLVFKDQYIQLSSSLPKD 223

Query: 194 RSSIFGIGEQIRESFKLVSNKSKTLTLWNGDVGRAVVDVNLYGSHPFYIDVRSPSRDGKV 253
           RSS+FGIGEQ R+SFK+V +KSKTLTLW+ D+G    DVNLYG+HPFYIDVRSPSRDGKV
Sbjct: 224 RSSLFGIGEQTRKSFKIVPDKSKTLTLWSADIGSVNTDVNLYGAHPFYIDVRSPSRDGKV 283

Query: 254 VAGTTHGVLLLNSNGMDVIYSGDRITYKVIGGVIDLYFFAGPSPVSVIDQYTELIGRPAP 313
            AGTTHGVLLLNSNGMD+IYSGDRI+YK IGG+IDLYFFAGPSP+SVIDQYTELIGRPAP
Sbjct: 284 AAGTTHGVLLLNSNGMDIIYSGDRISYKAIGGIIDLYFFAGPSPISVIDQYTELIGRPAP 343

Query: 314 VPYWSFGYHQCRYGYKNVSDVENVVARYAKAGIPLEVVCTDIDYMDEYKDFTFDPINFPV 373
           VPYWSFG+HQCRYGYKNVSD+E VVARYAKAGIPLEV+ TDIDYMD YKDFTFDPINFPV
Sbjct: 344 VPYWSFGFHQCRYGYKNVSDIETVVARYAKAGIPLEVMWTDIDYMDGYKDFTFDPINFPV 403

Query: 374 EKMKIFVDNLHQNGQKYVPILDPGISVNKTYGTFVRGTKADIFIKYDGVPYSGEVWSGPV 433
           EKMK FVD+LHQNGQKYV I+DPGIS NKTYGT++RG +ADIFIKYDGVPY GEVW GPV
Sbjct: 404 EKMKNFVDSLHQNGQKYVLIVDPGISTNKTYGTYIRGIEADIFIKYDGVPYLGEVWPGPV 463

Query: 434 YFPDFVHPNSEIFWGGEIKLFQDMVSFDGLWIDMNEISNAITSSTSPLSNLDNPPYKINN 493
           YFPDF+HPNSEIFWGGEIKLF+D+V FDGLWIDMNE+SN ITSSTSP SNLDNPPYKI+N
Sbjct: 464 YFPDFLHPNSEIFWGGEIKLFRDIVPFDGLWIDMNELSNFITSSTSPFSNLDNPPYKIDN 523

Query: 494 AAVLRPINNMTVPASSLHFGNLTEYNTHNLYGLLESRATHASLVKVTGKRPFVLSRSTFV 553
           A V RPINN TVPASSLHFGNLTEYNTHNLYG LES+ATHASLVKVTGKRPFVLSRSTFV
Sbjct: 524 AGVQRPINNRTVPASSLHFGNLTEYNTHNLYGFLESKATHASLVKVTGKRPFVLSRSTFV 583

Query: 554 SSGKYTAHWTGDNAATWNHLGYTIPSILNFGLFGIPMVGSDICGFSGDTTEELCRRWIQL 613
            SGKYTAHWTGDNAATWN LGYTIPSILNFGLFGIPMVG+DICGFSGDTTEELCRRWIQL
Sbjct: 584 GSGKYTAHWTGDNAATWNDLGYTIPSILNFGLFGIPMVGADICGFSGDTTEELCRRWIQL 643

Query: 614 GAFYPFARGHSDKLYIRRELYLWDSVAATARKVLALRYRLFPYFYTLMYEAHRKGTPIAR 673
           GAFYPFAR HSDK  IR+ELYLWD+VAA+ARKVLALRY L PY YTLMYEAH+KGTPIAR
Sbjct: 644 GAFYPFARDHSDKGSIRQELYLWDTVAASARKVLALRYSLLPYIYTLMYEAHKKGTPIAR 703

Query: 674 PLFFSFPQDVETYEISSQFLLGQGVLISPVLMEGAVSVDAYFPAGNWFSLFNYSESVSVK 733
           PLFFSFPQD+ TYEI+SQFLLG+GVL+SPVL EGA+SVDAYFPAGNWFSLFNYSESV+VK
Sbjct: 704 PLFFSFPQDIRTYEINSQFLLGEGVLVSPVLKEGALSVDAYFPAGNWFSLFNYSESVAVK 763

Query: 734 SGQQVTLDAPADHINVHVREGNILALHGDAMTTRAARETAFKLLVVVSNGRSSNGEVFLD 793
           SGQQ+TLDAPADHINVHVREGNILALHG+A TT+AAR+TAFKLLVVVSNG+SS+GEVFLD
Sbjct: 764 SGQQITLDAPADHINVHVREGNILALHGEATTTQAARKTAFKLLVVVSNGQSSSGEVFLD 823

Query: 794 DGEVVEMGEEGGNWSLVRFYSEAVGNKLLIKSQVINGGFALSQKLIIDKVTFVGFEWPKK 853
           DGEVVEMGEEGGNWSLVRFYSEAVG+KLLIKSQVINGGFALSQK+IIDKVTFVGFE PKK
Sbjct: 824 DGEVVEMGEEGGNWSLVRFYSEAVGSKLLIKSQVINGGFALSQKMIIDKVTFVGFERPKK 883

Query: 854 MGGFGLKISKGANLNGNSGIRKTYEYSGKFVNVEISALSIPIWEEFKLELT 896
           MGG GL ISKGANLNGNSGIRKTYEYS KFVNVEIS LSIPI EEF +EL+
Sbjct: 884 MGGLGLDISKGANLNGNSGIRKTYEYSAKFVNVEISGLSIPICEEFVMELS 934

BLAST of MC03g_new0279 vs. NCBI nr
Match: XP_023520110.1 (alpha-glucosidase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1523 bits (3944), Expect = 0.0
Identity = 746/886 (84.20%), Postives = 800/886 (90.29%), Query Frame = 0

Query: 18  CFF-------FSLVEPQPSPTIGYGYRIRSVHVDPAGKSLTADLALIKTSSVYGPDLHTL 77
           CFF       F+  E + +  +GYGYR+RS  VDP GKSLTADL LI+ SSVYGPD+  L
Sbjct: 49  CFFRLTASLPFAAGEQRENSAVGYGYRVRSGRVDPTGKSLTADLDLIEKSSVYGPDVERL 108

Query: 78  TLQASFETKDRLRIRITDSTRERWEIPDHIIPRRSNSRFRSPPETAVDSPATAFISDPAS 137
           ++QASFETKDRLR+RITDS RERWE+PD IIPRRSNSR RS PE  V SP T+ ISDPAS
Sbjct: 109 SIQASFETKDRLRVRITDSNRERWEVPDEIIPRRSNSRIRSLPERDVGSPETSVISDPAS 168

Query: 138 DLSFSLRNTTPFGFSVLRRSSGDVLFDTAPDLSDPGTFLVFKDQYIQLSSSLPKDRSSIF 197
           DL FSL +T PFGFSV RRSSGDVLFDT+P+ SD  TFLVFKDQYIQLSSSLPKDRSS+F
Sbjct: 169 DLIFSLHDTAPFGFSVHRRSSGDVLFDTSPNFSDSETFLVFKDQYIQLSSSLPKDRSSLF 228

Query: 198 GIGEQIRESFKLVSNKSKTLTLWNGDVGRAVVDVNLYGSHPFYIDVRSPSRDGKVVAGTT 257
           GIGEQ R+SFKLV +KSKTLTLWN D+G    DVNLYG+HPFYIDVRSPSRDGKV AGTT
Sbjct: 229 GIGEQTRKSFKLVPDKSKTLTLWNADIGSVNTDVNLYGAHPFYIDVRSPSRDGKVAAGTT 288

Query: 258 HGVLLLNSNGMDVIYSGDRITYKVIGGVIDLYFFAGPSPVSVIDQYTELIGRPAPVPYWS 317
           HGVLLLNSNGMD+IYSGDRI+YK IGG+IDLYFFAGPSP+SVIDQYTELIGRPAPVPYWS
Sbjct: 289 HGVLLLNSNGMDIIYSGDRISYKAIGGIIDLYFFAGPSPISVIDQYTELIGRPAPVPYWS 348

Query: 318 FGYHQCRYGYKNVSDVENVVARYAKAGIPLEVVCTDIDYMDEYKDFTFDPINFPVEKMKI 377
           FG+HQCRYGYKNVSD+E VVARYAKAGIPLEV+ TDIDYMD YKDFTFDPINFPVEKMK 
Sbjct: 349 FGFHQCRYGYKNVSDIETVVARYAKAGIPLEVMWTDIDYMDGYKDFTFDPINFPVEKMKN 408

Query: 378 FVDNLHQNGQKYVPILDPGISVNKTYGTFVRGTKADIFIKYDGVPYSGEVWSGPVYFPDF 437
           FVDNLHQNGQKYV ILDPGIS NKTYGT++RG +ADIFIKYDGVPY GEVW GPVYFPDF
Sbjct: 409 FVDNLHQNGQKYVLILDPGISTNKTYGTYIRGIEADIFIKYDGVPYLGEVWPGPVYFPDF 468

Query: 438 VHPNSEIFWGGEIKLFQDMVSFDGLWIDMNEISNAITSSTSPLSNLDNPPYKINNAAVLR 497
           +HPNSEIFWG EIKLF+D+V FDGLWIDMNEISN ITSSTS  SNLDNPPYKINNA V R
Sbjct: 469 LHPNSEIFWGDEIKLFRDIVPFDGLWIDMNEISNFITSSTSSFSNLDNPPYKINNAGVQR 528

Query: 498 PINNMTVPASSLHFGNLTEYNTHNLYGLLESRATHASLVKVTGKRPFVLSRSTFVSSGKY 557
           PINN TVPASSLHFGNLTEYNTHNLYG LES+ATHASLVKVTGKRPFVLSRSTFV SGKY
Sbjct: 529 PINNRTVPASSLHFGNLTEYNTHNLYGFLESKATHASLVKVTGKRPFVLSRSTFVGSGKY 588

Query: 558 TAHWTGDNAATWNHLGYTIPSILNFGLFGIPMVGSDICGFSGDTTEELCRRWIQLGAFYP 617
           TAHWTGDNAATWN LGYTIPSILNFGLFGIPMVG+DICGFS DTTEELCRRWIQLGAFYP
Sbjct: 589 TAHWTGDNAATWNDLGYTIPSILNFGLFGIPMVGADICGFSRDTTEELCRRWIQLGAFYP 648

Query: 618 FARGHSDKLYIRRELYLWDSVAATARKVLALRYRLFPYFYTLMYEAHRKGTPIARPLFFS 677
           FAR HSDK  IR+ELYLWD+VAA+ARKVLALRY L PY YTLMYEAH+KGTPIARPLFFS
Sbjct: 649 FARDHSDKGSIRQELYLWDTVAASARKVLALRYSLLPYIYTLMYEAHKKGTPIARPLFFS 708

Query: 678 FPQDVETYEISSQFLLGQGVLISPVLMEGAVSVDAYFPAGNWFSLFNYSESVSVKSGQQV 737
           FPQD+ TYEI+SQFLLG GVLISPVL EGA+SVDAYFPAGNWFSLFNYSESV+V SGQQ+
Sbjct: 709 FPQDIRTYEINSQFLLGAGVLISPVLKEGAISVDAYFPAGNWFSLFNYSESVAVNSGQQI 768

Query: 738 TLDAPADHINVHVREGNILALHGDAMTTRAARETAFKLLVVVSNGRSSNGEVFLDDGEVV 797
           TLDAPADHINVHVREGNILALHG+A TT+AAR+TAFKLLVVVSNG+SS+GEVFLDDGEVV
Sbjct: 769 TLDAPADHINVHVREGNILALHGEATTTQAARKTAFKLLVVVSNGQSSSGEVFLDDGEVV 828

Query: 798 EMGEEGGNWSLVRFYSEAVGNKLLIKSQVINGGFALSQKLIIDKVTFVGFEWPKKMGGFG 857
           EMGEEGGNWSLVRFYSEAVG+KLLIKSQVINGGFALSQK+IIDKVT VGFE PK MGG G
Sbjct: 829 EMGEEGGNWSLVRFYSEAVGSKLLIKSQVINGGFALSQKMIIDKVTIVGFERPKNMGGLG 888

Query: 858 LKISKGANLNGNSGIRKTYEYSGKFVNVEISALSIPIWEEFKLELT 896
           L ISKGANL+GNSGIRKTYEYS KFVNVEIS LSI IWEEF +EL+
Sbjct: 889 LDISKGANLDGNSGIRKTYEYSAKFVNVEISGLSISIWEEFVMELS 934

BLAST of MC03g_new0279 vs. NCBI nr
Match: KAG6583815.1 (hypothetical protein SDJN03_19747, partial [Cucurbita argyrosperma subsp. sororia] >KAG7019441.1 hypothetical protein SDJN02_18402 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1519 bits (3933), Expect = 0.0
Identity = 742/886 (83.75%), Postives = 799/886 (90.18%), Query Frame = 0

Query: 18  CFF-------FSLVEPQPSPTIGYGYRIRSVHVDPAGKSLTADLALIKTSSVYGPDLHTL 77
           CFF       F+  E + +  +GYGYR+RS  VDP GKSLTADL LI+ SSVYGPD+  L
Sbjct: 28  CFFRLTASLPFAAGEKRENSAVGYGYRVRSGRVDPTGKSLTADLDLIEKSSVYGPDVERL 87

Query: 78  TLQASFETKDRLRIRITDSTRERWEIPDHIIPRRSNSRFRSPPETAVDSPATAFISDPAS 137
           +LQASFETKDRLR+RITDS RERWEIPD IIPRRSNSR RS PE  V SP T+ ISDPAS
Sbjct: 88  SLQASFETKDRLRVRITDSNRERWEIPDEIIPRRSNSRIRSLPERDVGSPETSVISDPAS 147

Query: 138 DLSFSLRNTTPFGFSVLRRSSGDVLFDTAPDLSDPGTFLVFKDQYIQLSSSLPKDRSSIF 197
           DL FSL +T PFGFSV RRSSGDVLFDT+P+ SD  TFLVFKDQYIQLSSSLPKDRSS+F
Sbjct: 148 DLIFSLHDTAPFGFSVHRRSSGDVLFDTSPNFSDLETFLVFKDQYIQLSSSLPKDRSSLF 207

Query: 198 GIGEQIRESFKLVSNKSKTLTLWNGDVGRAVVDVNLYGSHPFYIDVRSPSRDGKVVAGTT 257
           GIGEQ R+SFKLV +KSKTLTLWN D+G    DVNLYG+HPFYIDVRSPSRDGKV AGTT
Sbjct: 208 GIGEQTRKSFKLVPDKSKTLTLWNADIGSVNTDVNLYGAHPFYIDVRSPSRDGKVAAGTT 267

Query: 258 HGVLLLNSNGMDVIYSGDRITYKVIGGVIDLYFFAGPSPVSVIDQYTELIGRPAPVPYWS 317
           HGVLLLNSNGMD+IY+GDRI+YK IGG+IDLYFFAGPSP+SVIDQYTELIGRPAPVPYWS
Sbjct: 268 HGVLLLNSNGMDIIYTGDRISYKAIGGIIDLYFFAGPSPISVIDQYTELIGRPAPVPYWS 327

Query: 318 FGYHQCRYGYKNVSDVENVVARYAKAGIPLEVVCTDIDYMDEYKDFTFDPINFPVEKMKI 377
           FG+HQCRYGYKNVSD+E VV+RYAKAGIPLEV+ TDIDYMD YKDFTFDPINFPVEKMK 
Sbjct: 328 FGFHQCRYGYKNVSDIETVVSRYAKAGIPLEVMWTDIDYMDGYKDFTFDPINFPVEKMKN 387

Query: 378 FVDNLHQNGQKYVPILDPGISVNKTYGTFVRGTKADIFIKYDGVPYSGEVWSGPVYFPDF 437
           FVDNLHQNGQKYV ILDPGIS NKTYGT++RG +ADIFIKYDGVPY GEVW GPVYFPDF
Sbjct: 388 FVDNLHQNGQKYVLILDPGISTNKTYGTYIRGIEADIFIKYDGVPYLGEVWPGPVYFPDF 447

Query: 438 VHPNSEIFWGGEIKLFQDMVSFDGLWIDMNEISNAITSSTSPLSNLDNPPYKINNAAVLR 497
           +HPNSEIFWG EIKLF+++V FDGLWIDMNEISN ITSSTS  SNLDNPPYKINNA V R
Sbjct: 448 LHPNSEIFWGDEIKLFREIVPFDGLWIDMNEISNFITSSTSSFSNLDNPPYKINNAGVQR 507

Query: 498 PINNMTVPASSLHFGNLTEYNTHNLYGLLESRATHASLVKVTGKRPFVLSRSTFVSSGKY 557
           PINN TVPASSLHFGNLTEYNTHNLYG LES+ATHASLVKVTGKRPFVLSRSTFV SGKY
Sbjct: 508 PINNRTVPASSLHFGNLTEYNTHNLYGFLESKATHASLVKVTGKRPFVLSRSTFVGSGKY 567

Query: 558 TAHWTGDNAATWNHLGYTIPSILNFGLFGIPMVGSDICGFSGDTTEELCRRWIQLGAFYP 617
           TAHWTGDNAATWN LGYTIPSILNFGLFGIPMVG+DICGFSGDTTEELCRRWIQLGAFYP
Sbjct: 568 TAHWTGDNAATWNDLGYTIPSILNFGLFGIPMVGADICGFSGDTTEELCRRWIQLGAFYP 627

Query: 618 FARGHSDKLYIRRELYLWDSVAATARKVLALRYRLFPYFYTLMYEAHRKGTPIARPLFFS 677
           FAR HSDK  IR+ELYLWD+VAA+ARKVLALRY L PY YTLMYEAH+KGTPIARPLFFS
Sbjct: 628 FARDHSDKGSIRQELYLWDTVAASARKVLALRYSLLPYIYTLMYEAHKKGTPIARPLFFS 687

Query: 678 FPQDVETYEISSQFLLGQGVLISPVLMEGAVSVDAYFPAGNWFSLFNYSESVSVKSGQQV 737
           FPQD+ TYEI+SQFLLG GVLISPVL EGA+SVDAYFPAGNWFSLFNYSESV++KSGQQ+
Sbjct: 688 FPQDIRTYEINSQFLLGAGVLISPVLKEGAISVDAYFPAGNWFSLFNYSESVAIKSGQQI 747

Query: 738 TLDAPADHINVHVREGNILALHGDAMTTRAARETAFKLLVVVSNGRSSNGEVFLDDGEVV 797
           TLDAPADHINVHVREGNILALHG+A TT+AAR+T FKLLVVVSNG+SS+GEVFLDDGE +
Sbjct: 748 TLDAPADHINVHVREGNILALHGEATTTQAARKTEFKLLVVVSNGQSSSGEVFLDDGEEM 807

Query: 798 EMGEEGGNWSLVRFYSEAVGNKLLIKSQVINGGFALSQKLIIDKVTFVGFEWPKKMGGFG 857
           EMGEEGGNWSLVRFY EAVG+KLLIKSQVINGGFALSQK+IIDKVT VGFE PK MGG G
Sbjct: 808 EMGEEGGNWSLVRFYGEAVGSKLLIKSQVINGGFALSQKMIIDKVTIVGFERPKNMGGLG 867

Query: 858 LKISKGANLNGNSGIRKTYEYSGKFVNVEISALSIPIWEEFKLELT 896
           L ISKGANLNGNSGIRKTYE+S KFVNVEIS LSIPIWEEF +E +
Sbjct: 868 LDISKGANLNGNSGIRKTYEHSAKFVNVEISGLSIPIWEEFVMEFS 913

BLAST of MC03g_new0279 vs. NCBI nr
Match: XP_022927400.1 (alpha-glucosidase [Cucurbita moschata])

HSP 1 Score: 1509 bits (3908), Expect = 0.0
Identity = 740/886 (83.52%), Postives = 795/886 (89.73%), Query Frame = 0

Query: 18  CFF-------FSLVEPQPSPTIGYGYRIRSVHVDPAGKSLTADLALIKTSSVYGPDLHTL 77
           CFF       F+  E + +  +GYGYR+RS  VDP GKSLTADL LI  SSVYGPD+  L
Sbjct: 28  CFFRLTASLPFAAGEQRENSAVGYGYRVRSGRVDPTGKSLTADLDLIDKSSVYGPDVERL 87

Query: 78  TLQASFETKDRLRIRITDSTRERWEIPDHIIPRRSNSRFRSPPETAVDSPATAFISDPAS 137
           +LQASFETKDRLR+RITDS RERWE+PD IIPRRSNSR RS PE  V SP T+ ISDPAS
Sbjct: 88  SLQASFETKDRLRVRITDSNRERWEVPDEIIPRRSNSRIRSLPERDVGSPETSVISDPAS 147

Query: 138 DLSFSLRNTTPFGFSVLRRSSGDVLFDTAPDLSDPGTFLVFKDQYIQLSSSLPKDRSSIF 197
           DL FSL +T PFGFSV RRSSGDVLFDT+P+ SD  TFLVFKDQYIQLSSSLPKDRSS+F
Sbjct: 148 DLIFSLHDTAPFGFSVHRRSSGDVLFDTSPNFSDSETFLVFKDQYIQLSSSLPKDRSSLF 207

Query: 198 GIGEQIRESFKLVSNKSKTLTLWNGDVGRAVVDVNLYGSHPFYIDVRSPSRDGKVVAGTT 257
           GIGEQ R+SFKLV +KSKTLTLWN D+G    DVNLYG+HPFYIDVRSPSRDGKV AGTT
Sbjct: 208 GIGEQTRKSFKLVPDKSKTLTLWNADIGSVNTDVNLYGAHPFYIDVRSPSRDGKVAAGTT 267

Query: 258 HGVLLLNSNGMDVIYSGDRITYKVIGGVIDLYFFAGPSPVSVIDQYTELIGRPAPVPYWS 317
           HGVLLLNSNGMD+IY+GDRI+YK IGG+IDLYFFAGPSP+SVIDQYTELIGRPAPVPYWS
Sbjct: 268 HGVLLLNSNGMDIIYTGDRISYKAIGGIIDLYFFAGPSPISVIDQYTELIGRPAPVPYWS 327

Query: 318 FGYHQCRYGYKNVSDVENVVARYAKAGIPLEVVCTDIDYMDEYKDFTFDPINFPVEKMKI 377
           FG+HQCRYGYKNVSD+E VVARYAKAGIPLEV+ TDIDYMD YKDFTFDPINFPVEKMK 
Sbjct: 328 FGFHQCRYGYKNVSDIETVVARYAKAGIPLEVMWTDIDYMDGYKDFTFDPINFPVEKMKN 387

Query: 378 FVDNLHQNGQKYVPILDPGISVNKTYGTFVRGTKADIFIKYDGVPYSGEVWSGPVYFPDF 437
           FVDNLHQNGQKYV ILDPGIS NKTYGT++RG +ADIFIKYDGVPY GEVW GPVYFPDF
Sbjct: 388 FVDNLHQNGQKYVLILDPGISTNKTYGTYIRGIEADIFIKYDGVPYLGEVWPGPVYFPDF 447

Query: 438 VHPNSEIFWGGEIKLFQDMVSFDGLWIDMNEISNAITSSTSPLSNLDNPPYKINNAAVLR 497
           +HPNSEIFWG EIKL +D+V FDGLWIDMNEISN ITSSTS  SNLDNPPYKINNA V R
Sbjct: 448 LHPNSEIFWGDEIKLLRDIVPFDGLWIDMNEISNFITSSTSSFSNLDNPPYKINNAGVQR 507

Query: 498 PINNMTVPASSLHFGNLTEYNTHNLYGLLESRATHASLVKVTGKRPFVLSRSTFVSSGKY 557
           PINN TVPASSLHFGNLTEYNTHNLYG LES+ATHASLVKVTGKRPFVLSRSTFV SGKY
Sbjct: 508 PINNRTVPASSLHFGNLTEYNTHNLYGFLESKATHASLVKVTGKRPFVLSRSTFVGSGKY 567

Query: 558 TAHWTGDNAATWNHLGYTIPSILNFGLFGIPMVGSDICGFSGDTTEELCRRWIQLGAFYP 617
           TAHWTGDNAATWN LGYTIPSILNFGLFGIPMVG+DICGFSGDTTEELCRRWIQLGAFYP
Sbjct: 568 TAHWTGDNAATWNDLGYTIPSILNFGLFGIPMVGADICGFSGDTTEELCRRWIQLGAFYP 627

Query: 618 FARGHSDKLYIRRELYLWDSVAATARKVLALRYRLFPYFYTLMYEAHRKGTPIARPLFFS 677
           FAR HSDK  IR+ELYLWD+VAA+ARKVLALRY L PY YTLMYEAH+KGTPIARPLFFS
Sbjct: 628 FARDHSDKGSIRQELYLWDTVAASARKVLALRYSLLPYIYTLMYEAHKKGTPIARPLFFS 687

Query: 678 FPQDVETYEISSQFLLGQGVLISPVLMEGAVSVDAYFPAGNWFSLFNYSESVSVKSGQQV 737
           FPQD+ TYEI+SQFLLG GVLISPVL EGA+SVDAYFPAGNWFSLFNYSESV++KSGQQ+
Sbjct: 688 FPQDIRTYEINSQFLLGAGVLISPVLKEGAISVDAYFPAGNWFSLFNYSESVAIKSGQQI 747

Query: 738 TLDAPADHINVHVREGNILALHGDAMTTRAARETAFKLLVVVSNGRSSNGEVFLDDGEVV 797
           TLDAPADHINVHVREGNILALHG+A TT+AAR+T FKLLVVVSNG+SS+GEVFLDDGE +
Sbjct: 748 TLDAPADHINVHVREGNILALHGEATTTQAARKTEFKLLVVVSNGQSSSGEVFLDDGEEM 807

Query: 798 EMGEEGGNWSLVRFYSEAVGNKLLIKSQVINGGFALSQKLIIDKVTFVGFEWPKKMGGFG 857
           EMGEEGGNWSLVRFYSEAVG+KLLIKSQVINGGFALSQK+IIDKVT VGFE PK MG  G
Sbjct: 808 EMGEEGGNWSLVRFYSEAVGSKLLIKSQVINGGFALSQKMIIDKVTIVGFERPKNMGDLG 867

Query: 858 LKISKGANLNGNSGIRKTYEYSGKFVNVEISALSIPIWEEFKLELT 896
           L ISKGANLNGNSGIR TYEYS KFVNV+IS LSIPI E F +EL+
Sbjct: 868 LDISKGANLNGNSGIRITYEYSAKFVNVQISGLSIPICEGFVMELS 913

BLAST of MC03g_new0279 vs. ExPASy TrEMBL
Match: A0A6J1CNJ7 (alpha-glucosidase-like OS=Momordica charantia OX=3673 GN=LOC111012738 PE=3 SV=1)

HSP 1 Score: 1652 bits (4277), Expect = 0.0
Identity = 818/900 (90.89%), Postives = 852/900 (94.67%), Query Frame = 0

Query: 1   MAKTATICIFGFPLFI-ICFFF-SLVEPQPSPTIGYGYRIRSVHVDPAGKSLTADLALIK 60
           M +TAT    G   FI ICFFF  L +P P P  G+GYRIRSV + P GKSLTADLALI 
Sbjct: 27  MQRTATRATSGLSFFILICFFFLCLAQPPPPPPAGHGYRIRSVQLHPTGKSLTADLALIG 86

Query: 61  TSS-VYGPDLHTLTLQASFETKDRLRIRITDSTRERWEIPDHIIPRRSNSRFRSPPETAV 120
           TSS VYGPDL TLTLQASFETKDRLRIRITDS RERWEIPD+IIPRRSNSRFRS PE+ V
Sbjct: 87  TSSSVYGPDLPTLTLQASFETKDRLRIRITDSARERWEIPDNIIPRRSNSRFRSLPESHV 146

Query: 121 DSPATAFISDPASDLSFSLRNTTPFGFSVLRRSSGDVLFDTAPDLSDPGTFLVFKDQYIQ 180
           DSPATAFISDPASDL+FSLRNTTPFGFSVLRRSSGDVLFDTAPDLSDPGTFLVFKDQYIQ
Sbjct: 147 DSPATAFISDPASDLAFSLRNTTPFGFSVLRRSSGDVLFDTAPDLSDPGTFLVFKDQYIQ 206

Query: 181 LSSSLPKDRSSIFGIGEQIRESFKLVSNKSKTLTLWNGDVGRAVVDVNLYGSHPFYIDVR 240
           LSS LP DRSS+FGIGEQ RESFKLVSNKSKTLTLWN D+GRA+VDVNLYGSHPFYIDVR
Sbjct: 207 LSSLLPNDRSSLFGIGEQTRESFKLVSNKSKTLTLWNADIGRAIVDVNLYGSHPFYIDVR 266

Query: 241 SPSRDGKVVAGTTHGVLLLNSNGMDVIYSGDRITYKVIGGVIDLYFFAGPSPVSVIDQYT 300
           SPSRDGKVVAGTTHGVLLLNSNGMD+IYSGDRITYKVIGGVIDLYFFAGPSPVSVIDQYT
Sbjct: 267 SPSRDGKVVAGTTHGVLLLNSNGMDIIYSGDRITYKVIGGVIDLYFFAGPSPVSVIDQYT 326

Query: 301 ELIGRPAPVPYWSFGYHQCRYGYKNVSDVENVVARYAKAGIPLEVVCTDIDYMDEYKDFT 360
           ELIGRPAPVPYWSFG+HQCRYGYKNVSDVENVVARYAKAGIPLEV+ TDIDYMD YKDFT
Sbjct: 327 ELIGRPAPVPYWSFGFHQCRYGYKNVSDVENVVARYAKAGIPLEVMWTDIDYMDGYKDFT 386

Query: 361 FDPINFPVEKMKIFVDNLHQNGQKYVPILDPGISVNKTYGTFVRGTKADIFIKYDGVPYS 420
           FDPINFP EKMKIFVDNLHQNGQKYV ILDPGIS+NKTYGTF+RGTKADIFIKY+GVPYS
Sbjct: 387 FDPINFPAEKMKIFVDNLHQNGQKYVAILDPGISINKTYGTFIRGTKADIFIKYNGVPYS 446

Query: 421 GEVWSGPVYFPDFVHPNSEIFWGGEIKLFQDMVSFDGLWIDMNEISNAITSSTSPLSNLD 480
           GEVWSGPVYFPDFVHPNSEIFWGGEIKLF+D+V FDGLWIDMNEISNAITSSTSPLSNLD
Sbjct: 447 GEVWSGPVYFPDFVHPNSEIFWGGEIKLFRDIVPFDGLWIDMNEISNAITSSTSPLSNLD 506

Query: 481 NPPYKINNAAVLRPINNMTVPASSLHFGNLTEYNTHNLYGLLESRATHASLVKVTGKRPF 540
           NPPYKINNAAVLRPINN TVPASSLHFGNLTEYNTHNLYGLLESRATHASLVKVTGKRPF
Sbjct: 507 NPPYKINNAAVLRPINNNTVPASSLHFGNLTEYNTHNLYGLLESRATHASLVKVTGKRPF 566

Query: 541 VLSRSTFVSSGKYTAHWTGDNAATWNHLGYTIPSILNFGLFGIPMVGSDICGFSGDTTEE 600
           V+SRSTFV SGKYTAHWTGDN ATWN LGYTIPSILNFGLFGIPMVGSDICGF+GDTTEE
Sbjct: 567 VVSRSTFVGSGKYTAHWTGDNDATWNDLGYTIPSILNFGLFGIPMVGSDICGFAGDTTEE 626

Query: 601 LCRRWIQLGAFYPFARGHSDKLYIRRELYLWDSVAATARKVLALRYRLFPYFYTLMYEAH 660
           LCRRWIQLGAFYPFARGHSDK YIRRELYLWDSVAATARKVLALRYRL PYFYTLMYEAH
Sbjct: 627 LCRRWIQLGAFYPFARGHSDKFYIRRELYLWDSVAATARKVLALRYRLLPYFYTLMYEAH 686

Query: 661 RKGTPIARPLFFSFPQDVETYEISSQFLLGQGVLISPVLMEGAVSVDAYFPAGNWFSLFN 720
           RKGTPIARPLFFSFPQD++TYEISSQFLLG+GVLISPVL EGA+SVDAYFP GNWFSLFN
Sbjct: 687 RKGTPIARPLFFSFPQDIKTYEISSQFLLGKGVLISPVLKEGALSVDAYFPEGNWFSLFN 746

Query: 721 YSESVSVKSGQQVTLDAPADHINVHVREGNILALHGDAMTTRAARETAFKLLVVVSNGRS 780
           YSESVSVKSGQQVTLDAPADHINVHVREGNIL LHGDAMTTRAAR TAFKLLVVVSNG+S
Sbjct: 747 YSESVSVKSGQQVTLDAPADHINVHVREGNILVLHGDAMTTRAARGTAFKLLVVVSNGQS 806

Query: 781 SNGEVFLDDGEVVEMGEEGGNWSLVRFYSEAVGNKLLIKSQVINGGFALSQKLIIDKVTF 840
           S+GEVFLDDG VVEMGEEGGNWSLVRF+SEAVG+KLLIKS+V+NGGFAL+QKLIIDKVTF
Sbjct: 807 SDGEVFLDDGGVVEMGEEGGNWSLVRFHSEAVGSKLLIKSEVVNGGFALTQKLIIDKVTF 866

Query: 841 VGFEWPKKMGGFGLKISKGANLNGNSGIRKTYEYSGKFVNVEISALSIPIWEEFKLELTF 897
           +GFE  +KMGGFGLKISKGANLNGNS I+KTYEYSGKFVNVEISALSIPIWEEFKLELTF
Sbjct: 867 IGFERHEKMGGFGLKISKGANLNGNSAIKKTYEYSGKFVNVEISALSIPIWEEFKLELTF 926

BLAST of MC03g_new0279 vs. ExPASy TrEMBL
Match: A0A6J1KFK1 (alpha-glucosidase OS=Cucurbita maxima OX=3661 GN=LOC111495351 PE=3 SV=1)

HSP 1 Score: 1531 bits (3965), Expect = 0.0
Identity = 749/891 (84.06%), Postives = 809/891 (90.80%), Query Frame = 0

Query: 14  LFIICFFFSLV--------EPQPSPTIGYGYRIRSVHVDPAGKSLTADLALIKTSSVYGP 73
           LF+   FF L+        E + +  +GYGYR+RS  VDP GKSLTADL LI+ SSVYGP
Sbjct: 44  LFLFSCFFRLIASLPFAAGEQRENSAVGYGYRVRSGRVDPTGKSLTADLDLIEKSSVYGP 103

Query: 74  DLHTLTLQASFETKDRLRIRITDSTRERWEIPDHIIPRRSNSRFRSPPETAVDSPATAFI 133
           D+  L+LQASFETKDRLR+RITDS RERWEIPD IIPRRSNSR RS PE  V SP T+ I
Sbjct: 104 DVERLSLQASFETKDRLRVRITDSNRERWEIPDEIIPRRSNSRIRSLPERDVGSPETSII 163

Query: 134 SDPASDLSFSLRNTTPFGFSVLRRSSGDVLFDTAPDLSDPGTFLVFKDQYIQLSSSLPKD 193
           SDPASDL FSL +T PFGFSV RRSSGDVLFDT+P+ SD  TFLVFKDQYIQLSSSLPKD
Sbjct: 164 SDPASDLIFSLHDTAPFGFSVHRRSSGDVLFDTSPNFSDSETFLVFKDQYIQLSSSLPKD 223

Query: 194 RSSIFGIGEQIRESFKLVSNKSKTLTLWNGDVGRAVVDVNLYGSHPFYIDVRSPSRDGKV 253
           RSS+FGIGEQ R+SFK+V +KSKTLTLW+ D+G    DVNLYG+HPFYIDVRSPSRDGKV
Sbjct: 224 RSSLFGIGEQTRKSFKIVPDKSKTLTLWSADIGSVNTDVNLYGAHPFYIDVRSPSRDGKV 283

Query: 254 VAGTTHGVLLLNSNGMDVIYSGDRITYKVIGGVIDLYFFAGPSPVSVIDQYTELIGRPAP 313
            AGTTHGVLLLNSNGMD+IYSGDRI+YK IGG+IDLYFFAGPSP+SVIDQYTELIGRPAP
Sbjct: 284 AAGTTHGVLLLNSNGMDIIYSGDRISYKAIGGIIDLYFFAGPSPISVIDQYTELIGRPAP 343

Query: 314 VPYWSFGYHQCRYGYKNVSDVENVVARYAKAGIPLEVVCTDIDYMDEYKDFTFDPINFPV 373
           VPYWSFG+HQCRYGYKNVSD+E VVARYAKAGIPLEV+ TDIDYMD YKDFTFDPINFPV
Sbjct: 344 VPYWSFGFHQCRYGYKNVSDIETVVARYAKAGIPLEVMWTDIDYMDGYKDFTFDPINFPV 403

Query: 374 EKMKIFVDNLHQNGQKYVPILDPGISVNKTYGTFVRGTKADIFIKYDGVPYSGEVWSGPV 433
           EKMK FVD+LHQNGQKYV I+DPGIS NKTYGT++RG +ADIFIKYDGVPY GEVW GPV
Sbjct: 404 EKMKNFVDSLHQNGQKYVLIVDPGISTNKTYGTYIRGIEADIFIKYDGVPYLGEVWPGPV 463

Query: 434 YFPDFVHPNSEIFWGGEIKLFQDMVSFDGLWIDMNEISNAITSSTSPLSNLDNPPYKINN 493
           YFPDF+HPNSEIFWGGEIKLF+D+V FDGLWIDMNE+SN ITSSTSP SNLDNPPYKI+N
Sbjct: 464 YFPDFLHPNSEIFWGGEIKLFRDIVPFDGLWIDMNELSNFITSSTSPFSNLDNPPYKIDN 523

Query: 494 AAVLRPINNMTVPASSLHFGNLTEYNTHNLYGLLESRATHASLVKVTGKRPFVLSRSTFV 553
           A V RPINN TVPASSLHFGNLTEYNTHNLYG LES+ATHASLVKVTGKRPFVLSRSTFV
Sbjct: 524 AGVQRPINNRTVPASSLHFGNLTEYNTHNLYGFLESKATHASLVKVTGKRPFVLSRSTFV 583

Query: 554 SSGKYTAHWTGDNAATWNHLGYTIPSILNFGLFGIPMVGSDICGFSGDTTEELCRRWIQL 613
            SGKYTAHWTGDNAATWN LGYTIPSILNFGLFGIPMVG+DICGFSGDTTEELCRRWIQL
Sbjct: 584 GSGKYTAHWTGDNAATWNDLGYTIPSILNFGLFGIPMVGADICGFSGDTTEELCRRWIQL 643

Query: 614 GAFYPFARGHSDKLYIRRELYLWDSVAATARKVLALRYRLFPYFYTLMYEAHRKGTPIAR 673
           GAFYPFAR HSDK  IR+ELYLWD+VAA+ARKVLALRY L PY YTLMYEAH+KGTPIAR
Sbjct: 644 GAFYPFARDHSDKGSIRQELYLWDTVAASARKVLALRYSLLPYIYTLMYEAHKKGTPIAR 703

Query: 674 PLFFSFPQDVETYEISSQFLLGQGVLISPVLMEGAVSVDAYFPAGNWFSLFNYSESVSVK 733
           PLFFSFPQD+ TYEI+SQFLLG+GVL+SPVL EGA+SVDAYFPAGNWFSLFNYSESV+VK
Sbjct: 704 PLFFSFPQDIRTYEINSQFLLGEGVLVSPVLKEGALSVDAYFPAGNWFSLFNYSESVAVK 763

Query: 734 SGQQVTLDAPADHINVHVREGNILALHGDAMTTRAARETAFKLLVVVSNGRSSNGEVFLD 793
           SGQQ+TLDAPADHINVHVREGNILALHG+A TT+AAR+TAFKLLVVVSNG+SS+GEVFLD
Sbjct: 764 SGQQITLDAPADHINVHVREGNILALHGEATTTQAARKTAFKLLVVVSNGQSSSGEVFLD 823

Query: 794 DGEVVEMGEEGGNWSLVRFYSEAVGNKLLIKSQVINGGFALSQKLIIDKVTFVGFEWPKK 853
           DGEVVEMGEEGGNWSLVRFYSEAVG+KLLIKSQVINGGFALSQK+IIDKVTFVGFE PKK
Sbjct: 824 DGEVVEMGEEGGNWSLVRFYSEAVGSKLLIKSQVINGGFALSQKMIIDKVTFVGFERPKK 883

Query: 854 MGGFGLKISKGANLNGNSGIRKTYEYSGKFVNVEISALSIPIWEEFKLELT 896
           MGG GL ISKGANLNGNSGIRKTYEYS KFVNVEIS LSIPI EEF +EL+
Sbjct: 884 MGGLGLDISKGANLNGNSGIRKTYEYSAKFVNVEISGLSIPICEEFVMELS 934

BLAST of MC03g_new0279 vs. ExPASy TrEMBL
Match: A0A6J1ENT6 (alpha-glucosidase OS=Cucurbita moschata OX=3662 GN=LOC111434234 PE=3 SV=1)

HSP 1 Score: 1509 bits (3908), Expect = 0.0
Identity = 740/886 (83.52%), Postives = 795/886 (89.73%), Query Frame = 0

Query: 18  CFF-------FSLVEPQPSPTIGYGYRIRSVHVDPAGKSLTADLALIKTSSVYGPDLHTL 77
           CFF       F+  E + +  +GYGYR+RS  VDP GKSLTADL LI  SSVYGPD+  L
Sbjct: 28  CFFRLTASLPFAAGEQRENSAVGYGYRVRSGRVDPTGKSLTADLDLIDKSSVYGPDVERL 87

Query: 78  TLQASFETKDRLRIRITDSTRERWEIPDHIIPRRSNSRFRSPPETAVDSPATAFISDPAS 137
           +LQASFETKDRLR+RITDS RERWE+PD IIPRRSNSR RS PE  V SP T+ ISDPAS
Sbjct: 88  SLQASFETKDRLRVRITDSNRERWEVPDEIIPRRSNSRIRSLPERDVGSPETSVISDPAS 147

Query: 138 DLSFSLRNTTPFGFSVLRRSSGDVLFDTAPDLSDPGTFLVFKDQYIQLSSSLPKDRSSIF 197
           DL FSL +T PFGFSV RRSSGDVLFDT+P+ SD  TFLVFKDQYIQLSSSLPKDRSS+F
Sbjct: 148 DLIFSLHDTAPFGFSVHRRSSGDVLFDTSPNFSDSETFLVFKDQYIQLSSSLPKDRSSLF 207

Query: 198 GIGEQIRESFKLVSNKSKTLTLWNGDVGRAVVDVNLYGSHPFYIDVRSPSRDGKVVAGTT 257
           GIGEQ R+SFKLV +KSKTLTLWN D+G    DVNLYG+HPFYIDVRSPSRDGKV AGTT
Sbjct: 208 GIGEQTRKSFKLVPDKSKTLTLWNADIGSVNTDVNLYGAHPFYIDVRSPSRDGKVAAGTT 267

Query: 258 HGVLLLNSNGMDVIYSGDRITYKVIGGVIDLYFFAGPSPVSVIDQYTELIGRPAPVPYWS 317
           HGVLLLNSNGMD+IY+GDRI+YK IGG+IDLYFFAGPSP+SVIDQYTELIGRPAPVPYWS
Sbjct: 268 HGVLLLNSNGMDIIYTGDRISYKAIGGIIDLYFFAGPSPISVIDQYTELIGRPAPVPYWS 327

Query: 318 FGYHQCRYGYKNVSDVENVVARYAKAGIPLEVVCTDIDYMDEYKDFTFDPINFPVEKMKI 377
           FG+HQCRYGYKNVSD+E VVARYAKAGIPLEV+ TDIDYMD YKDFTFDPINFPVEKMK 
Sbjct: 328 FGFHQCRYGYKNVSDIETVVARYAKAGIPLEVMWTDIDYMDGYKDFTFDPINFPVEKMKN 387

Query: 378 FVDNLHQNGQKYVPILDPGISVNKTYGTFVRGTKADIFIKYDGVPYSGEVWSGPVYFPDF 437
           FVDNLHQNGQKYV ILDPGIS NKTYGT++RG +ADIFIKYDGVPY GEVW GPVYFPDF
Sbjct: 388 FVDNLHQNGQKYVLILDPGISTNKTYGTYIRGIEADIFIKYDGVPYLGEVWPGPVYFPDF 447

Query: 438 VHPNSEIFWGGEIKLFQDMVSFDGLWIDMNEISNAITSSTSPLSNLDNPPYKINNAAVLR 497
           +HPNSEIFWG EIKL +D+V FDGLWIDMNEISN ITSSTS  SNLDNPPYKINNA V R
Sbjct: 448 LHPNSEIFWGDEIKLLRDIVPFDGLWIDMNEISNFITSSTSSFSNLDNPPYKINNAGVQR 507

Query: 498 PINNMTVPASSLHFGNLTEYNTHNLYGLLESRATHASLVKVTGKRPFVLSRSTFVSSGKY 557
           PINN TVPASSLHFGNLTEYNTHNLYG LES+ATHASLVKVTGKRPFVLSRSTFV SGKY
Sbjct: 508 PINNRTVPASSLHFGNLTEYNTHNLYGFLESKATHASLVKVTGKRPFVLSRSTFVGSGKY 567

Query: 558 TAHWTGDNAATWNHLGYTIPSILNFGLFGIPMVGSDICGFSGDTTEELCRRWIQLGAFYP 617
           TAHWTGDNAATWN LGYTIPSILNFGLFGIPMVG+DICGFSGDTTEELCRRWIQLGAFYP
Sbjct: 568 TAHWTGDNAATWNDLGYTIPSILNFGLFGIPMVGADICGFSGDTTEELCRRWIQLGAFYP 627

Query: 618 FARGHSDKLYIRRELYLWDSVAATARKVLALRYRLFPYFYTLMYEAHRKGTPIARPLFFS 677
           FAR HSDK  IR+ELYLWD+VAA+ARKVLALRY L PY YTLMYEAH+KGTPIARPLFFS
Sbjct: 628 FARDHSDKGSIRQELYLWDTVAASARKVLALRYSLLPYIYTLMYEAHKKGTPIARPLFFS 687

Query: 678 FPQDVETYEISSQFLLGQGVLISPVLMEGAVSVDAYFPAGNWFSLFNYSESVSVKSGQQV 737
           FPQD+ TYEI+SQFLLG GVLISPVL EGA+SVDAYFPAGNWFSLFNYSESV++KSGQQ+
Sbjct: 688 FPQDIRTYEINSQFLLGAGVLISPVLKEGAISVDAYFPAGNWFSLFNYSESVAIKSGQQI 747

Query: 738 TLDAPADHINVHVREGNILALHGDAMTTRAARETAFKLLVVVSNGRSSNGEVFLDDGEVV 797
           TLDAPADHINVHVREGNILALHG+A TT+AAR+T FKLLVVVSNG+SS+GEVFLDDGE +
Sbjct: 748 TLDAPADHINVHVREGNILALHGEATTTQAARKTEFKLLVVVSNGQSSSGEVFLDDGEEM 807

Query: 798 EMGEEGGNWSLVRFYSEAVGNKLLIKSQVINGGFALSQKLIIDKVTFVGFEWPKKMGGFG 857
           EMGEEGGNWSLVRFYSEAVG+KLLIKSQVINGGFALSQK+IIDKVT VGFE PK MG  G
Sbjct: 808 EMGEEGGNWSLVRFYSEAVGSKLLIKSQVINGGFALSQKMIIDKVTIVGFERPKNMGDLG 867

Query: 858 LKISKGANLNGNSGIRKTYEYSGKFVNVEISALSIPIWEEFKLELT 896
           L ISKGANLNGNSGIR TYEYS KFVNV+IS LSIPI E F +EL+
Sbjct: 868 LDISKGANLNGNSGIRITYEYSAKFVNVQISGLSIPICEGFVMELS 913

BLAST of MC03g_new0279 vs. ExPASy TrEMBL
Match: A0A5D3E1Q3 (Alpha-glucosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold129G00330 PE=3 SV=1)

HSP 1 Score: 1498 bits (3879), Expect = 0.0
Identity = 730/886 (82.39%), Postives = 797/886 (89.95%), Query Frame = 0

Query: 14  LFIICFFFSLVEPQPS---PTIGYGYRIRSVHVDPAGKSLTADLALIKTSSVYGPDLHTL 73
           + I+  F S + P P+   P +G+GYRIRS H+DPAGKSLTADL LI+TS VYGPDL TL
Sbjct: 21  ILILFLFTSFLIPPPAASLPAVGFGYRIRSSHIDPAGKSLTADLQLIRTSQVYGPDLPTL 80

Query: 74  TLQASFETKDRLRIRITDSTRERWEIPDHIIPRRSNSRFRSPPETAVDSPATAFISDPAS 133
           TLQA+FE+KDRLR+RITDSTRERWEIPDHIIPR SNS  RS PE  V SP  +FISDPAS
Sbjct: 81  TLQATFESKDRLRVRITDSTRERWEIPDHIIPRPSNSLIRSLPENHVASPKPSFISDPAS 140

Query: 134 DLSFSLRNTTPFGFSVLRRSSGDVLFDTAPDLSDPGTFLVFKDQYIQLSSSLPKDRSSIF 193
           DL F+L +T PFGFSVLRRSSGDVLFDT+PD S+  TF+VFKDQYIQLSS LPKDRSSIF
Sbjct: 141 DLIFTLYDTAPFGFSVLRRSSGDVLFDTSPDFSNSETFIVFKDQYIQLSSLLPKDRSSIF 200

Query: 194 GIGEQIRESFKLVSNKSKTLTLWNGDVGRAVVDVNLYGSHPFYIDVRSPSRDGKVVAGTT 253
           GIGEQ R+SFKLV +K+KTLTLWN D+G   +DVNLYG+HPFYIDVRS SRDGKVVAGTT
Sbjct: 201 GIGEQTRKSFKLVPDKNKTLTLWNADIGSVNLDVNLYGAHPFYIDVRSRSRDGKVVAGTT 260

Query: 254 HGVLLLNSNGMDVIYSGDRITYKVIGGVIDLYFFAGPSPVSVIDQYTELIGRPAPVPYWS 313
           HGVLLLNSNGMD+IYSGDRITYKVIGG+IDLYFFAGPSP+SVIDQYTELIGRPAPVPYWS
Sbjct: 261 HGVLLLNSNGMDIIYSGDRITYKVIGGIIDLYFFAGPSPISVIDQYTELIGRPAPVPYWS 320

Query: 314 FGYHQCRYGYKNVSDVENVVARYAKAGIPLEVVCTDIDYMDEYKDFTFDPINFPVEKMKI 373
           FG+HQCRYGYKNVS+VE+VVARYAKA IPLEV+ TDIDYMD YKDFTFDPINFP EKMKI
Sbjct: 321 FGFHQCRYGYKNVSEVESVVARYAKASIPLEVMWTDIDYMDGYKDFTFDPINFPSEKMKI 380

Query: 374 FVDNLHQNGQKYVPILDPGISVNKTYGTFVRGTKADIFIKYDGVPYSGEVWSGPVYFPDF 433
           FVDNLH+NGQKYV ILDPGIS N TYG ++RGTKADIF+K+DGVPY G+VW GPVYFPDF
Sbjct: 381 FVDNLHKNGQKYVIILDPGISTNNTYGPYIRGTKADIFMKHDGVPYLGDVWPGPVYFPDF 440

Query: 434 VHPNSEIFWGGEIKLFQDMVSFDGLWIDMNEISNAITSSTSPLSNLDNPPYKINNAAVLR 493
           +HP+SE FWG EI++F+D+V FDGLWIDMNEISN ITSSTSPLSNLDNPPY INNA V R
Sbjct: 441 LHPDSETFWGREIQIFRDIVPFDGLWIDMNEISNFITSSTSPLSNLDNPPYMINNARVRR 500

Query: 494 PINNMTVPASSLHFGNLTEYNTHNLYGLLESRATHASLVKVTGKRPFVLSRSTFVSSGKY 553
           P+NN TVPAS LHFGNLTEYNTHNLYG LESRATHASLVKVTGKRPFVLSRSTF  SGKY
Sbjct: 501 PLNNKTVPASILHFGNLTEYNTHNLYGFLESRATHASLVKVTGKRPFVLSRSTFAGSGKY 560

Query: 554 TAHWTGDNAATWNHLGYTIPSILNFGLFGIPMVGSDICGFSGDTTEELCRRWIQLGAFYP 613
           TAHWTGD  ATWN +GYTIPSILNFGLFGIPMVGSDICGFSGDTTEELCRRWIQLGAFYP
Sbjct: 561 TAHWTGDIGATWNDMGYTIPSILNFGLFGIPMVGSDICGFSGDTTEELCRRWIQLGAFYP 620

Query: 614 FARGHSDKLYIRRELYLWDSVAATARKVLALRYRLFPYFYTLMYEAHRKGTPIARPLFFS 673
           FAR HSDK  IR+ELYLWDSVAA+ARKVLALRY+L PYFYTLMYEAH+KGTPIARPLFFS
Sbjct: 621 FARDHSDKGSIRQELYLWDSVAASARKVLALRYQLLPYFYTLMYEAHKKGTPIARPLFFS 680

Query: 674 FPQDVETYEISSQFLLGQGVLISPVLMEGAVSVDAYFPAGNWFSLFNYSESVSVKSGQQV 733
           FPQD++TYEI SQFLLG+GVL+SPVL EGA SVDAYFPAGNWFSLFNYSE V+V SGQQ+
Sbjct: 681 FPQDIKTYEIDSQFLLGEGVLVSPVLKEGAFSVDAYFPAGNWFSLFNYSEFVAVNSGQQI 740

Query: 734 TLDAPADHINVHVREGNILALHGDAMTTRAARETAFKLLVVVSNGRSSNGEVFLDDGEVV 793
            LDAPADHINVHVREGNILALHG+AMTT+AARETA+KLLVV+SNG+ S GEVFLDDGEV 
Sbjct: 741 NLDAPADHINVHVREGNILALHGEAMTTQAARETAYKLLVVISNGQRSFGEVFLDDGEVA 800

Query: 794 EMGEEGGNWSLVRFYSEAVGNKLLIKSQVINGGFALSQKLIIDKVTFVGFEWPKKMGGFG 853
           EMG EGGNWS+VRF SE VG+KL++KSQVINGGFALSQKLIIDKVTFVGFE PKKM G G
Sbjct: 801 EMGGEGGNWSMVRFCSETVGSKLVVKSQVINGGFALSQKLIIDKVTFVGFERPKKMVGLG 860

Query: 854 LKISKGANLNGNSGIRKTYEYSGKFVNVEISALSIPIWEEFKLELT 896
           L ISKG +LNGNS IRKTY+Y  KF+NVEIS LSIPIWEEF LE+T
Sbjct: 861 LNISKGVSLNGNSSIRKTYQYFAKFMNVEISGLSIPIWEEFILEMT 906

BLAST of MC03g_new0279 vs. ExPASy TrEMBL
Match: A0A1S3C8V0 (alpha-glucosidase OS=Cucumis melo OX=3656 GN=LOC103498289 PE=3 SV=1)

HSP 1 Score: 1498 bits (3879), Expect = 0.0
Identity = 730/886 (82.39%), Postives = 797/886 (89.95%), Query Frame = 0

Query: 14  LFIICFFFSLVEPQPS---PTIGYGYRIRSVHVDPAGKSLTADLALIKTSSVYGPDLHTL 73
           + I+  F S + P P+   P +G+GYRIRS H+DPAGKSLTADL LI+TS VYGPDL TL
Sbjct: 21  ILILFLFTSFLIPPPAASLPAVGFGYRIRSSHIDPAGKSLTADLQLIRTSQVYGPDLPTL 80

Query: 74  TLQASFETKDRLRIRITDSTRERWEIPDHIIPRRSNSRFRSPPETAVDSPATAFISDPAS 133
           TLQA+FE+KDRLR+RITDSTRERWEIPDHIIPR SNS  RS PE  V SP  +FISDPAS
Sbjct: 81  TLQATFESKDRLRVRITDSTRERWEIPDHIIPRPSNSLIRSLPENHVASPKPSFISDPAS 140

Query: 134 DLSFSLRNTTPFGFSVLRRSSGDVLFDTAPDLSDPGTFLVFKDQYIQLSSSLPKDRSSIF 193
           DL F+L +T PFGFSVLRRSSGDVLFDT+PD S+  TF+VFKDQYIQLSS LPKDRSSIF
Sbjct: 141 DLIFTLYDTAPFGFSVLRRSSGDVLFDTSPDFSNSETFIVFKDQYIQLSSLLPKDRSSIF 200

Query: 194 GIGEQIRESFKLVSNKSKTLTLWNGDVGRAVVDVNLYGSHPFYIDVRSPSRDGKVVAGTT 253
           GIGEQ R+SFKLV +K+KTLTLWN D+G   +DVNLYG+HPFYIDVRS SRDGKVVAGTT
Sbjct: 201 GIGEQTRKSFKLVPDKNKTLTLWNADIGSVNLDVNLYGAHPFYIDVRSRSRDGKVVAGTT 260

Query: 254 HGVLLLNSNGMDVIYSGDRITYKVIGGVIDLYFFAGPSPVSVIDQYTELIGRPAPVPYWS 313
           HGVLLLNSNGMD+IYSGDRITYKVIGG+IDLYFFAGPSP+SVIDQYTELIGRPAPVPYWS
Sbjct: 261 HGVLLLNSNGMDIIYSGDRITYKVIGGIIDLYFFAGPSPISVIDQYTELIGRPAPVPYWS 320

Query: 314 FGYHQCRYGYKNVSDVENVVARYAKAGIPLEVVCTDIDYMDEYKDFTFDPINFPVEKMKI 373
           FG+HQCRYGYKNVS+VE+VVARYAKA IPLEV+ TDIDYMD YKDFTFDPINFP EKMKI
Sbjct: 321 FGFHQCRYGYKNVSEVESVVARYAKASIPLEVMWTDIDYMDGYKDFTFDPINFPSEKMKI 380

Query: 374 FVDNLHQNGQKYVPILDPGISVNKTYGTFVRGTKADIFIKYDGVPYSGEVWSGPVYFPDF 433
           FVDNLH+NGQKYV ILDPGIS N TYG ++RGTKADIF+K+DGVPY G+VW GPVYFPDF
Sbjct: 381 FVDNLHKNGQKYVIILDPGISTNNTYGPYIRGTKADIFMKHDGVPYLGDVWPGPVYFPDF 440

Query: 434 VHPNSEIFWGGEIKLFQDMVSFDGLWIDMNEISNAITSSTSPLSNLDNPPYKINNAAVLR 493
           +HP+SE FWG EI++F+D+V FDGLWIDMNEISN ITSSTSPLSNLDNPPY INNA V R
Sbjct: 441 LHPDSETFWGREIQIFRDIVPFDGLWIDMNEISNFITSSTSPLSNLDNPPYMINNARVRR 500

Query: 494 PINNMTVPASSLHFGNLTEYNTHNLYGLLESRATHASLVKVTGKRPFVLSRSTFVSSGKY 553
           P+NN TVPAS LHFGNLTEYNTHNLYG LESRATHASLVKVTGKRPFVLSRSTF  SGKY
Sbjct: 501 PLNNKTVPASILHFGNLTEYNTHNLYGFLESRATHASLVKVTGKRPFVLSRSTFAGSGKY 560

Query: 554 TAHWTGDNAATWNHLGYTIPSILNFGLFGIPMVGSDICGFSGDTTEELCRRWIQLGAFYP 613
           TAHWTGD  ATWN +GYTIPSILNFGLFGIPMVGSDICGFSGDTTEELCRRWIQLGAFYP
Sbjct: 561 TAHWTGDIGATWNDMGYTIPSILNFGLFGIPMVGSDICGFSGDTTEELCRRWIQLGAFYP 620

Query: 614 FARGHSDKLYIRRELYLWDSVAATARKVLALRYRLFPYFYTLMYEAHRKGTPIARPLFFS 673
           FAR HSDK  IR+ELYLWDSVAA+ARKVLALRY+L PYFYTLMYEAH+KGTPIARPLFFS
Sbjct: 621 FARDHSDKGSIRQELYLWDSVAASARKVLALRYQLLPYFYTLMYEAHKKGTPIARPLFFS 680

Query: 674 FPQDVETYEISSQFLLGQGVLISPVLMEGAVSVDAYFPAGNWFSLFNYSESVSVKSGQQV 733
           FPQD++TYEI SQFLLG+GVL+SPVL EGA SVDAYFPAGNWFSLFNYSE V+V SGQQ+
Sbjct: 681 FPQDIKTYEIDSQFLLGEGVLVSPVLKEGAFSVDAYFPAGNWFSLFNYSEFVAVNSGQQI 740

Query: 734 TLDAPADHINVHVREGNILALHGDAMTTRAARETAFKLLVVVSNGRSSNGEVFLDDGEVV 793
            LDAPADHINVHVREGNILALHG+AMTT+AARETA+KLLVV+SNG+ S GEVFLDDGEV 
Sbjct: 741 NLDAPADHINVHVREGNILALHGEAMTTQAARETAYKLLVVISNGQRSFGEVFLDDGEVA 800

Query: 794 EMGEEGGNWSLVRFYSEAVGNKLLIKSQVINGGFALSQKLIIDKVTFVGFEWPKKMGGFG 853
           EMG EGGNWS+VRF SE VG+KL++KSQVINGGFALSQKLIIDKVTFVGFE PKKM G G
Sbjct: 801 EMGGEGGNWSMVRFCSETVGSKLVVKSQVINGGFALSQKLIIDKVTFVGFERPKKMVGLG 860

Query: 854 LKISKGANLNGNSGIRKTYEYSGKFVNVEISALSIPIWEEFKLELT 896
           L ISKG +LNGNS IRKTY+Y  KF+NVEIS LSIPIWEEF LE+T
Sbjct: 861 LNISKGVSLNGNSSIRKTYQYFAKFMNVEISGLSIPIWEEFILEMT 906

BLAST of MC03g_new0279 vs. TAIR 10
Match: AT5G11720.1 (Glycosyl hydrolases family 31 protein )

HSP 1 Score: 1133.2 bits (2930), Expect = 0.0e+00
Identity = 555/891 (62.29%), Postives = 688/891 (77.22%), Query Frame = 0

Query: 16  IICFFFSL------VEPQPSPTIGYGYRIRSVHVDPAGKSLTADLALIKTSSVYGPDLHT 75
           ++  FFSL      +E + S  +GYGY +RSV VD   + LTA L LIK SSVY PD+ +
Sbjct: 13  VVVVFFSLRSSQVVLEEEESTVVGYGYVVRSVGVDSNRQVLTAKLDLIKPSSVYAPDIKS 72

Query: 76  LTLQASFETKDRLRIRITDSTRERWEIPDHIIPRRSNSRFR--SPPETAVDSPATAFISD 135
           L L  S ET +RLRIRITDS+++RWEIP+ +IPR  N   R  S  E   +SP   F++D
Sbjct: 73  LNLHVSLETSERLRIRITDSSQQRWEIPETVIPRAGNHSPRRFSTEEDGGNSPENNFLAD 132

Query: 136 PASDLSFSLRNTTPFGFSVLRRSSGDVLFDTAPDLSDPGTFLVFKDQYIQLSSSLPKDRS 195
           P+SDL F+L NTTPFGFSV RRSSGD+LFDT+PD SD  T+ +FKDQ++QLSS+LP++RS
Sbjct: 133 PSSDLVFTLHNTTPFGFSVSRRSSGDILFDTSPDSSDSNTYFIFKDQFLQLSSALPENRS 192

Query: 196 SIFGIGEQIRESFKLVSNKSKTLTLWNGDVGRAVVDVNLYGSHPFYIDVRSPSRDGKVVA 255
           +++GIGE  + SF+L+    +T+TLWN D+G    DVNLYGSHPFY+DVR     G   A
Sbjct: 193 NLYGIGEHTKRSFRLI--PGETMTLWNADIGSENPDVNLYGSHPFYMDVRGSK--GNEEA 252

Query: 256 GTTHGVLLLNSNGMDVIYSGDRITYKVIGGVIDLYFFAGPSPVSVIDQYTELIGRPAPVP 315
           GTTHGVLLLNSNGMDV Y G RITY VIGGVIDLY FAGPSP  V++QYTELIGRPAP+P
Sbjct: 253 GTTHGVLLLNSNGMDVKYEGHRITYNVIGGVIDLYVFAGPSPEMVMNQYTELIGRPAPMP 312

Query: 316 YWSFGYHQCRYGYKNVSDVENVVARYAKAGIPLEVVCTDIDYMDEYKDFTFDPINFPVEK 375
           YWSFG+HQCRYGYKNVSD+E VV  YAKAGIPLEV+ TDIDYMD YKDFT DP+NFP +K
Sbjct: 313 YWSFGFHQCRYGYKNVSDLEYVVDGYAKAGIPLEVMWTDIDYMDGYKDFTLDPVNFPEDK 372

Query: 376 MKIFVDNLHQNGQKYVPILDPGISVNKTYGTFVRGTKADIFIKYDGVPYSGEVWSGPVYF 435
           M+ FVD LH+NGQKYV ILDPGI V+ +YGT+ RG +AD+FIK +G PY GEVW G VYF
Sbjct: 373 MQSFVDTLHKNGQKYVLILDPGIGVDSSYGTYNRGMEADVFIKRNGEPYLGEVWPGKVYF 432

Query: 436 PDFVHPNSEIFWGGEIKLFQDMVSFDGLWIDMNEISNAITSSTSPLSNLDNPPYKINNAA 495
           PDF++P +  FW  EIK+FQ+++  DGLWIDMNE+SN ITS  S  S+LD+PPYKINN+ 
Sbjct: 433 PDFLNPAAATFWSNEIKMFQEILPLDGLWIDMNELSNFITSPLSSGSSLDDPPYKINNSG 492

Query: 496 VLRPINNMTVPASSLHFGNLTEYNTHNLYGLLESRATHASLVKVTGKRPFVLSRSTFVSS 555
             RPINN TVPA+S+HFGN++EY+ HNLYGLLE++ATH ++V +TGKRPF+LSRSTFVSS
Sbjct: 493 DKRPINNKTVPATSIHFGNISEYDAHNLYGLLEAKATHQAVVDITGKRPFILSRSTFVSS 552

Query: 556 GKYTAHWTGDNAATWNHLGYTIPSILNFGLFGIPMVGSDICGFSGDTTEELCRRWIQLGA 615
           GKYTAHWTGDNAA W  L Y+IP ILNFGLFGIPMVG+DICGFS DTTEELCRRWIQLGA
Sbjct: 553 GKYTAHWTGDNAAKWEDLAYSIPGILNFGLFGIPMVGADICGFSHDTTEELCRRWIQLGA 612

Query: 616 FYPFARGHSDKLYIRRELYLWDSVAATARKVLALRYRLFPYFYTLMYEAHRKGTPIARPL 675
           FYPFAR HS     R+ELYLWDSVA++ARKVL LR RL P+ YTLMYEAH  G PIARPL
Sbjct: 613 FYPFARDHSSLGTARQELYLWDSVASSARKVLGLRMRLLPHLYTLMYEAHVSGNPIARPL 672

Query: 676 FFSFPQDVETYEISSQFLLGQGVLISPVLMEGAVSVDAYFPAGNWFSLFNYSESVSVKSG 735
           FFSFPQD +TYEI SQFL+G+ +++SP L +GAV+VDAYFPAGNWF LFNYS +V   SG
Sbjct: 673 FFSFPQDTKTYEIDSQFLIGKSIMVSPALKQGAVAVDAYFPAGNWFDLFNYSFAVGGDSG 732

Query: 736 QQVTLDAPADHINVHVREGNILALHGDAMTTRAARETAFKLLVVVSNGRSSNGEVFLDDG 795
           + V LD PADH+NVHVREG+I+A+ G+A+TTR AR+T ++LLVV S   + +GE+FLDDG
Sbjct: 733 KHVRLDTPADHVNVHVREGSIVAMQGEALTTRDARKTPYQLLVVASRLENISGELFLDDG 792

Query: 796 EVVEMGEEGGN--WSLVRFYSEAVGNKLLIKSQVINGGFALSQKLIIDKVTFVGFEWPKK 855
           E + MG  GGN  W+LV+F     G  ++++S+V+N  +A   K  I KVTFVGFE  + 
Sbjct: 793 ENLRMGAGGGNRDWTLVKFRCYVTGKSVVLRSEVVNPEYASKMKWSIGKVTFVGFENVEN 852

Query: 856 MGGFGLKISKGANLNGNSGIRKTYEYSG-KFVNVEISALSIPIWEEFKLEL 896
           +  + ++ S+       S I+   +    +F++VE+S LS+ + ++F++ L
Sbjct: 853 VKTYEVRTSERLRSPRISLIKTVSDNDDPRFLSVEVSKLSLLVGKKFEMRL 899

BLAST of MC03g_new0279 vs. TAIR 10
Match: AT1G68560.1 (alpha-xylosidase 1 )

HSP 1 Score: 781.6 bits (2017), Expect = 7.0e-226
Identity = 414/930 (44.52%), Postives = 572/930 (61.51%), Query Frame = 0

Query: 1   MAKTATICIFGFPL---FIICFFFSLVEPQPSPTIGYGYRIRSVHVDPAGKSLTADLALI 60
           MA +++   F   L    I+CF       Q   TIG GYR+ S+   P G      L + 
Sbjct: 1   MASSSSSLAFSLSLLLALILCF----SPTQSYKTIGKGYRLVSIEESPDG-GFIGYLQVK 60

Query: 61  KTSSVYGPDLHTLTLQASFETKDRLRIRITDSTRERWEIPDHIIPRRSNSRFRSPPETAV 120
           + + +YG D+ TL L    ET  RLR+ ITD+ ++RWE+P +++PR    +       + 
Sbjct: 61  QKNKIYGSDITTLRLFVKHETDSRLRVHITDAKQQRWEVPYNLLPREQPPQVGKVIGKSR 120

Query: 121 DSPATAFISDPASDLSFSLRNTTPFGFSVLRRSSGDVLFDTAPDLSDPGTFLVFKDQYIQ 180
            SP T       S+L FS   T PF F+V RRS+ + LF+T        + LVFKDQY++
Sbjct: 121 KSPITV-QEISGSELIFSY-TTDPFTFAVKRRSNHETLFNTT-------SSLVFKDQYLE 180

Query: 181 LSSSLPKDRSSIFGIGEQIR-ESFKLVSNKSKTLTLWNGDVGRAVVDVNLYGSHPFYIDV 240
           +S+SLPK+ +S++G+GE  +    KLV N  +  TL+  DV    ++ +LYGSHP Y+D+
Sbjct: 181 ISTSLPKE-ASLYGLGENSQANGIKLVPN--EPYTLYTEDVSAINLNTDLYGSHPMYMDL 240

Query: 241 RSPSRDGKVVAGTTHGVLLLNSNGMDVIYSGDRITYKVIGGVIDLYFFAGPSPVSVIDQY 300
           R+    GK  A   H VLLLNSNGMDV Y GD +TYKVIGGV D YF AGPSP++V+DQY
Sbjct: 241 RNVG--GKAYA---HAVLLLNSNGMDVFYRGDSLTYKVIGGVFDFYFIAGPSPLNVVDQY 300

Query: 301 TELIGRPAPVPYWSFGYHQCRYGYKNVSDVENVVARYAKAGIPLEVVCTDIDYMDEYKDF 360
           T+LIGRPAP+PYWS G+HQCR+GY N+S VE+VV  Y KA IPL+V+  D D+MD +KDF
Sbjct: 301 TQLIGRPAPMPYWSLGFHQCRWGYHNLSVVEDVVDNYKKAKIPLDVIWNDDDHMDGHKDF 360

Query: 361 TFDPINFPVEKMKIFVDNLHQNGQKYVPILDPGISVNKTYGTFVRGTKADIFIKYDGVPY 420
           T +P+ +P  K+  F+D +H+ G KY+ I DPGI VN +YGTF R   AD+FIKY+G P+
Sbjct: 361 TLNPVAYPRAKLLAFLDKIHKIGMKYIVINDPGIGVNASYGTFQRAMAADVFIKYEGKPF 420

Query: 421 SGEVWSGPVYFPDFVHPNSEIFWGGEIKLFQDMVSFDGLWIDMNEISN------------ 480
             +VW GPVYFPDF++P +  +WG EIK F D+V  DGLWIDMNE+SN            
Sbjct: 421 LAQVWPGPVYFPDFLNPKTVSWWGDEIKRFHDLVPIDGLWIDMNEVSNFCSGLCTIPEGK 480

Query: 481 ------------AITSSTSPLSNLDNPPYKINNAAVLRPINNMTVPASSLHFGNLTEYNT 540
                        +       +  D+PPYKIN   V+ P+   T+  S+ H+  + EY+ 
Sbjct: 481 QCPSGEGPGWVCCLDCKNITKTRWDDPPYKINATGVVAPVGFKTIATSATHYNGVREYDA 540

Query: 541 HNLYGLLESRATHASLVKVTGKRPFVLSRSTFVSSGKYTAHWTGDNAATWNHLGYTIPSI 600
           H++YG  E+ ATH  L+ V GKRPF+LSRSTFV SG+Y AHWTGDN  TW  L  +I ++
Sbjct: 541 HSIYGFSETIATHKGLLNVQGKRPFILSRSTFVGSGQYAAHWTGDNQGTWQSLQVSISTM 600

Query: 601 LNFGLFGIPMVGSDICGFSGDTTEELCRRWIQLGAFYPFARGHSDKLYIRRELYLWDSVA 660
           LNFG+FG+PMVGSDICGF    TEELC RWI++GAFYPF+R H++    R+ELY WD+VA
Sbjct: 601 LNFGIFGVPMVGSDICGFYPQPTEELCNRWIEVGAFYPFSRDHANYYSPRQELYQWDTVA 660

Query: 661 ATARKVLALRYRLFPYFYTLMYEAHRKGTPIARPLFFSFPQDVETYEISSQFLLGQGVLI 720
            +AR  L +RY++ P+ YTL YEAH  G PIARPLFFSFP+  E Y  S QFLLG   +I
Sbjct: 661 DSARNALGMRYKILPFLYTLNYEAHMTGAPIARPLFFSFPEYTECYGNSRQFLLGSSFMI 720

Query: 721 SPVLMEGAVSVDAYFPAGNWFSLFNYSESVSVKSGQQVTLDAPADHINVHVREGNILALH 780
           SPVL +G   V+A FP G+W+ +F+ +++V  K+G++VTL AP + +NVH+ +  IL   
Sbjct: 721 SPVLEQGKTEVEALFPPGSWYHMFDMTQAVVSKNGKRVTLPAPLNFVNVHLYQNTILPTQ 780

Query: 781 GDAMTTRAARETAFKLLVVVSNGRS---SNGEVFLDDGEVVEMGEEGGNWSLVRFYSEAV 840
              + ++ AR T F L++    G S   + G+++LD+ E+ EM    G  + V FY+   
Sbjct: 781 QGGLISKDARTTPFSLVIAFPAGASEGYATGKLYLDEDELPEMKLGNGQSTYVDFYASVG 840

Query: 841 GNKLLIKSQVINGGFALSQKLIIDKVTFVGFEWPKKMGGFGLKISKGANLNGNSGIRKTY 894
              + + SQV  G FALS+  +I+KV+ +G     ++    +  S        S    TY
Sbjct: 841 NGTMKMWSQVKEGKFALSKGWVIEKVSVLGLRGAGQVSEIQINGSPMTKKIEVSSKEHTY 900

BLAST of MC03g_new0279 vs. TAIR 10
Match: AT3G45940.1 (Glycosyl hydrolases family 31 protein )

HSP 1 Score: 753.1 bits (1943), Expect = 2.6e-217
Identity = 402/872 (46.10%), Postives = 547/872 (62.73%), Query Frame = 0

Query: 8   CIFGFPLFIICFFFSLVEPQPSPTIGYGYRIRSVHVDPAGKSLTADLALIKTSSVYGPDL 67
           C+      I+CF       Q S  IG GYR+ S+   P   S    L + +++ +YG D+
Sbjct: 4   CLSLLVAIILCF----SSLQCSNAIGKGYRLISMEKSPDDGSFIGYLQVKQSNKIYGSDI 63

Query: 68  HTLTLQASFETKDRLRIRITDSTRERWEIPDHIIPRRSNSRFRSPPET---AVDSPATA- 127
             L L  ++ T  RLR+ ITD+ ++RWE+P +++ R        PP     +  SP T  
Sbjct: 64  TILRLFINYRTDHRLRVHITDAKKQRWEVPYNLLRR------EQPPNVIGKSRKSPVTVQ 123

Query: 128 FISDPASDLSFSLRNTTPFGFSVLRRSSGDVLFDTAPDLSDPGTFLVFKDQYIQLSSSLP 187
            IS P   L F++    PF F+V RRS+G+ +F+T+      G  +VFKDQY+++S+SLP
Sbjct: 124 EISGPELILIFTV---DPFSFAVRRRSNGETIFNTSSSDESFGE-MVFKDQYLEISTSLP 183

Query: 188 KDRSSIFGIGEQIR-ESFKLVSNKSKTLTLWNGDVGRAVVDVNLYGSHPFYIDVRSPSRD 247
           KD +S++G GE  +    KLV N  +  TL+  DV    ++ +LYGSHP Y+D+R+ S  
Sbjct: 184 KD-ASLYGFGENSQANGIKLVPN--EPYTLFTEDVSAFNLNTDLYGSHPVYMDLRNVS-- 243

Query: 248 GKVVAGTTHGVLLLNSNGMDVIYSGDRITYKVIGGVIDLYFFAGPSPVSVIDQYTELIGR 307
           GK  A   H VLLLNS+GMDV Y GD +TYKVIGGV D YFFAGPSP++V+DQYT LIGR
Sbjct: 244 GKAYA---HSVLLLNSHGMDVFYRGDSLTYKVIGGVFDFYFFAGPSPLNVVDQYTSLIGR 303

Query: 308 PAPVPYWSFGYHQCRYGYKNVSDVENVVARYAKAGIPLEVVCTDIDYMDEYKDFTFDPIN 367
           PAP+PYWS G+HQCR+GY+NVS V++VV  Y KA IPL+V+  D DYMD YKDFT D +N
Sbjct: 304 PAPMPYWSLGFHQCRWGYRNVSVVKDVVDNYQKAKIPLDVIWNDADYMDGYKDFTLDLVN 363

Query: 368 FPVEKMKIFVDNLHQNGQKYVPILDPGISVNKTYGTFVRGTKADIFIKYDGVPYSGEVWS 427
           FP  K+  F+D +H+ G KYV I DPGI VN +YG + RG  +D+FIKY+G P+  +VW 
Sbjct: 364 FPHAKLLSFLDRIHKMGMKYVVIKDPGIGVNASYGVYQRGMASDVFIKYEGKPFLAQVWP 423

Query: 428 GPVYFPDFVHPNSEIFWGGEIKLFQDMVSFDGLWIDMNEISNAITSSTSPLSNLDNPPYK 487
           GPVYFPDF++P +  +WG EI+ F ++V  DGLWIDMNEI NA     S           
Sbjct: 424 GPVYFPDFLNPKTVSWWGDEIRRFHELVPIDGLWIDMNEI-NATGHKAS----------- 483

Query: 488 INNAAVLRPINNMTVPASSLHFGNLTEYNTHNLYGLLESRATHASLVKVTGKRPFVLSRS 547
                    +   T+P S+ H+  + EY+ H++YG  E+ ATH +L+ V GKRPF+LSRS
Sbjct: 484 ---------LGFKTIPTSAYHYNGVREYDAHSIYGFSEAIATHKALLAVQGKRPFILSRS 543

Query: 548 TFVSSGKYTAHWTGDNAATWNHLGYTIPSILNFGLFGIPMVGSDICGFSGDTTEELCRRW 607
           TFV SG+Y AHWTGDN  TW  L  +I ++LNFG+FG+PMVGSDICGF   T EELC RW
Sbjct: 544 TFVGSGQYAAHWTGDNQGTWQSLQVSISTMLNFGIFGVPMVGSDICGFFPPTPEELCNRW 603

Query: 608 IQLGAFYPFARGHSDKLYIRRELYLWDSVAATARKVLALRYRLFPYFYTLMYEAHRKGTP 667
           I++GAFYPF+R H+D    R+ELY W +VA +AR  L +RY+L P+ YTL YEAH  G P
Sbjct: 604 IEVGAFYPFSRDHADYYAPRKELYQWGTVAESARNALGMRYKLLPFLYTLNYEAHMSGAP 663

Query: 668 IARPLFFSFPQDVETYEISSQFLLGQGVLISPVLMEGAVSVDAYFPAGNWFSLFNYSESV 727
           IARPLFFSFP+  E Y +S QFLLG  ++ISPVL +G   V+A FP G+W+ +F+ ++ V
Sbjct: 664 IARPLFFSFPEFTECYGLSKQFLLGSSLMISPVLEQGKTQVEALFPPGSWYHMFDMTQVV 723

Query: 728 SVKSGQQVTLDAPADHINVHVREGNILALHGDAMTTRAARETAFKLLVVVSNGRSSNGEV 787
             K+G+  TL AP + +NVH+ +  IL +         A E              ++G++
Sbjct: 724 VSKNGRLFTLPAPFNVVNVHLYQNAILPMQQVVAFPAGASE------------GYASGKL 783

Query: 788 FLDDGEVVEMGEEGGNWSLVRFYSEAVGNKLLIKSQVINGGFALSQKLIIDKVTFVGFEW 847
           FLDD E+ EM    G  + + FY+      + I SQV  G FALSQ L+I+KV  +G + 
Sbjct: 784 FLDDDELPEMKLGNGKSTYIDFYASVGNESVKIWSQVKEGQFALSQGLVIEKVIVLGLKG 814

Query: 848 PKKMGGFGLKISKGANLNGNSGIRKTYEYSGK 875
             K+      +  G++++  +   KT E S K
Sbjct: 844 TWKVSEI---LLNGSSISNET---KTIEVSSK 814

BLAST of MC03g_new0279 vs. TAIR 10
Match: AT5G63840.1 (Glycosyl hydrolases family 31 protein )

HSP 1 Score: 307.0 bits (785), Expect = 5.0e-83
Identity = 224/690 (32.46%), Postives = 329/690 (47.68%), Query Frame = 0

Query: 185 DRSSIFGIGEQIRESFKLVSNK------SKTLTLWNGDVGRAVVD--VNLYGSHPFYIDV 244
           D S ++GI E    SF L   K      S+   L+N DV     +    LYGS PF +  
Sbjct: 222 DSSFVYGIPEH-ATSFALKPTKGPGVEESEPYRLFNLDVFEYDHESPFGLYGSIPFMVS- 281

Query: 245 RSPSRDGKVVAGTTHGVLLLNSNGM--DVIYSG-------------DRIT--YKVIGGVI 304
                 GK  +G T G   LN+  M  DV+ +G              RI   +    G++
Sbjct: 282 -----HGK--SGKTSGFFWLNAAEMQIDVLANGWDAESGISLPSSHSRIDTFWMSEAGIV 341

Query: 305 DLYFFAGPSPVSVIDQYTELIGRPAPVPYWSFGYHQCRYGYKNVSDVENVVARYAKAGIP 364
           D +FF GP P  V+ QY  + G  A    ++ GYHQCR+ YK+  DV  V +++ +  IP
Sbjct: 342 DTFFFVGPEPKDVVKQYASVTGTSAMPQLFATGYHQCRWNYKDEEDVAQVDSKFDEHDIP 401

Query: 365 LEVVCTDIDYMDEYKDFTFDPINFP-VEKMKIFVDNLHQNGQKYVPILDPGISVNKTYGT 424
            +V+  DI++ D  + FT+D + FP  E+M+     L   G+K V I+DP I  + +Y  
Sbjct: 402 YDVLWLDIEHTDGKRYFTWDSVLFPHPEEMQ---KKLAAKGRKMVTIVDPHIKRDDSYFL 461

Query: 425 FVRGTKADIFIK-YDGVPYSGEVWSGPVYFPDFVHPNSEIFWGGEIKLFQDMVSFDGL-- 484
               T+   ++K   G  + G  W G   + D + P    +WGG       + S   L  
Sbjct: 462 HKEATQMGYYVKDSSGKDFDGWCWPGSSSYIDMLSPEIRKWWGGRFSYKNYVGSTPSLYT 521

Query: 485 WIDMNEISNAITSSTSPLSNLDNPPYKINNAAVLRPINNMTVPASSLHFGNLTEYNTHNL 544
           W DMNE S            + N P              +T+P  +LH G +     HN 
Sbjct: 522 WNDMNEPS------------VFNGP-------------EVTMPRDALHVGGVEHREVHNA 581

Query: 545 YGLLESRATHASLV-KVTGK-RPFVLSRSTFVSSGKYTAHWTGDNAATWNHLGYTIPSIL 604
           YG     AT   LV +  GK RPFVLSR+ F  + +Y A WTGDN A W HL  +IP IL
Sbjct: 582 YGYYFHMATSDGLVMREEGKDRPFVLSRAIFPGTQRYGAIWTGDNTAEWEHLRVSIPMIL 641

Query: 605 NFGLFGIPMVGSDICGFSGDTTEELCRRWIQLGAFYPFARGHSDKLYIRRELYLW-DSVA 664
             GL GI   G+DI GF G+   EL  RW Q+GA+YPF RGH+     RRE +L+ +   
Sbjct: 642 TLGLTGITFSGADIGGFFGNPEPELLVRWYQVGAYYPFFRGHAHHDTKRREPWLFGERNT 701

Query: 665 ATARKVLALRYRLFPYFYTLMYEAHRKGTPIARPLFFSFPQDVETYEISSQFLLGQGVLI 724
              R  +  RY L PYFYTL  EA+  G P+ RPL+  FPQD  T+     F++G G+L+
Sbjct: 702 ELMRDAIHTRYTLLPYFYTLFREANVTGVPVVRPLWMEFPQDEATFSNDEAFMVGSGLLV 761

Query: 725 SPVLMEGAVSVDAYFPA-GNWFSLFNYSESVSVKSGQQVTLDAPADHINVHVREGNILAL 784
             V  +G      Y P   +W+ L N    V    G+   +DAP + I    + G I+  
Sbjct: 762 QGVYTKGTTQASVYLPGKESWYDLRNGKTYV---GGKTHKMDAPEESIPAFQKAGTIIPR 821

Query: 785 HGDAMTTRAARET-AFKLLVVVSNGRSSNGEVFLDDGEVVEMGEEGGNWSLVRF-YSEAV 840
                 + +  +   + L+V +++ + + GE+++DDG+  E     G++   RF +S+ V
Sbjct: 822 KDRFRRSSSQMDNDPYTLVVALNSSQEAEGELYIDDGKSFEF--RRGSYIHRRFVFSKGV 865

BLAST of MC03g_new0279 vs. TAIR 10
Match: AT3G23640.1 (heteroglycan glucosidase 1 )

HSP 1 Score: 302.0 bits (772), Expect = 1.6e-81
Identity = 203/654 (31.04%), Postives = 316/654 (48.32%), Query Frame = 0

Query: 187 SSIFGIGEQIRESFKLVSNKSKTLTLWNGDV-GRAVVDVNLYGSHPFYIDVRSPSRDGKV 246
           +S +G GE   +    +    K +  WN D  G      +LY SHP+ + V        V
Sbjct: 86  TSFYGTGEVSGQ----LERTGKRVFTWNTDAWGYGSGTTSLYQSHPWVLVVLPTGETLGV 145

Query: 247 VAGTTHGVLL-LNSNGMDVIYSGDRITYKVIGGVIDLYFFAGPSPVSVIDQYTELIGRPA 306
           +A TT    + L   G+  I S    +Y +I       F    SP +V++  +  IG   
Sbjct: 146 LADTTRKCEIDLRKEGIIRIIS--PASYPII------TFGPFSSPTAVLESLSHAIGTVF 205

Query: 307 PVPYWSFGYHQCRYGYKNVSDVENVVARYAKAGIPLEVVCTDIDYMDEYKDFTFDPINFP 366
             P W+ GYHQCR+ Y +   V  +   +    IP +V+  DIDYMD ++ FTFD   FP
Sbjct: 206 MPPKWALGYHQCRWSYMSDKRVAEIAQTFRDKKIPSDVIWMDIDYMDGFRCFTFDKERFP 265

Query: 367 VEKMKIFVDNLHQNGQKYVPILDPGISVNKTYGTFVRGTKADIFI-KYDGVPYSGEVWSG 426
                    +LH NG K + +LDPGI   + Y  +  G+K D++I + DG P++GEVW G
Sbjct: 266 --DPSALAKDLHSNGFKAIWMLDPGIKQEEGYYVYDSGSKNDVWISRADGKPFTGEVWPG 325

Query: 427 PVYFPDFVHPNSEIFWGGEIKLFQDMVSFDGLWIDMNEISNAITSSTSPLSNLDNPPYKI 486
           P  FPD+ +  +  +W   +K F      DG+W DMNE                      
Sbjct: 326 PCVFPDYTNSKARSWWANLVKEFVSN-GVDGIWNDMNE---------------------- 385

Query: 487 NNAAVLRPINNMTVPASSLHFGN------LTEYNTHNLYGLLESRATHASL-VKVTGKRP 546
              AV + +   T+P +++H G+          + HN+YG+L +R+T+  + +    KRP
Sbjct: 386 --PAVFKVVTK-TMPENNIHHGDDELGGVQNHSHYHNVYGMLMARSTYEGMELADKNKRP 445

Query: 547 FVLSRSTFVSSGKYTAHWTGDNAATWNHLGYTIPSILNFGLFGIPMVGSDICGFSGDTTE 606
           FVL+R+ F+ S +Y A WTGDN + W HL  +I  +L  GL G P+ G DI GF+G+ T 
Sbjct: 446 FVLTRAGFIGSQRYAATWTGDNLSNWEHLHMSISMVLQLGLSGQPLSGPDIGGFAGNATP 505

Query: 607 ELCRRWIQLGAFYPFARGHSDKLYIRRELYLW-DSVAATARKVLALRYRLFPYFYTLMYE 666
            L  RW+ +GA +PF RGHS+      E + + +      R  L  RY+L P+FYTL Y 
Sbjct: 506 RLFGRWMGVGAMFPFCRGHSEAGTDDHEPWSFGEECEEVCRAALKRRYQLLPHFYTLFYI 565

Query: 667 AHRKGTPIARPLFFSFPQDVETYEISSQFLLGQGVLISPVL-MEGAVSVDAYFPAGNWFS 726
           AH  G P+A P+FF+ P D     + + FLLG  ++ +  L  +G+  +    P G W  
Sbjct: 566 AHTTGAPVAAPIFFADPIDSRLRAVENGFLLGPLLIYASTLSSQGSHELQHILPRGIWHR 625

Query: 727 LFNYSES------VSVKSGQQVTLDAPADHINVHVREGNILALHGDAMTTRAARETAFKL 786
            F++++S      + ++ G  ++L  P    ++HV E ++     D +T          L
Sbjct: 626 -FDFADSHPDLPTLYLQGGSIISLAPP----HLHVGEFSL----SDDLT----------L 678

Query: 787 LVVVSNGRSSNGEVFLDDGEVVEMGEEGGNWSLVRFYSEAVGNKLLIKSQVING 823
           LV +     + G +F DDG+    G   G + +  + +E   + + +K     G
Sbjct: 686 LVSLDENGKAKGLLFEDDGD--GYGYTKGRFLVTHYIAERDSSTVTVKVSKTEG 678