MC03g_new0253 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC03g_new0253
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	subtilisin-like protease SBT5.4
Location	MC03: 11474195 .. 11475420 (-)
RNA-Seq Expression	MC03g_new0253
Synteny	MC03g_new0253

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GTCTTGTATGGCTCAAACAAAAATACTTGAACACCGTTCATGATGGGATATGTGTACTAAAAAATTTCAAACTTCCTAATGGTATGATTCAATTTTTTCTAGGAAAATAGGGGGGTGATTTTGAAAATTTTATTTATTTAAATCTATTTTTTAAAAATCACTAATTATGTCACTCCTTAAACTTGTGCAGCCTGATGTGACGGCGCCTGGTGTGAACGTTATTGCTGCTTACACGGAAGCCGTGAGCCCCACCGGCGAGCCCTTCGACAACAGAACGGCTCCATTCATCACCATGTCCGGCACCTCCATGTCCTGCCCCCACGTCGCCGGACTCGCCGGCCTCCTCCGATCTCTGCATCCTAATTGGAGCCCTTCCGCCATTAAATCCGCAATCATGACCTCTGGTTCCTCAATTCACCCCATTATTGTTCAATTTAATCACACAATTTTACTATTAATTGATTGATTAATTAATCCCTAACCTGAAATCGAATTTGCGATCGCAGCCAGAATCAGAGACAACACAATGAAGCCGATGCTCGACGGCGGCACAGACGAACTCGCTCCGGCCACTCCTTTCAGCTACGGCTCCGGCCACATTCGCCCAATCGGAGCCGTCGATCCAGGCCTGGTTTACGATCTGACGCCCGACGATTACTTGGAATTTCTGTGCGCCATCGGATACGATGAGAAGAAGATCAGAGCATTCTCCGACGGTCCGTACAAATGTCCGCCTTCCGCCAGTCTTCTGAACTTCAATTACCCTTCGATCGGAGTTCAGAACATGACCGGAAGCGTTACGGTGACCAGAAGATTGAAGAACGTGGGCACTCCCGGAGTTTACAGAGCCAGAGTTCGGCAGCCGGAAGGAGTTAGGGTTTCGGTGGAGCCGAGATTTCTGAAATTCGATAAAGTTGGAGAGGAGAAGAGCTTCAAATTGACGATCGCCGGAGTTGTGCCGGCCAAACGCGTGGTCGACGGGACGCTGATTTGGACCGACGGCGAGCACTTCGTTAGGAGTCCTATCGTCATTTCTTCCGGCTTGTTCTGATTCTGAAGCATTTTTAGGATTTTTTATTTTTTATATTGAAGTTTTAGTTTTTTTTTTTCTTTCCTTTTTTGGGTTTGAGAGATATTAGTTTAATTTATATGCGATAATAAAATTCATTCTTTACATTTGAACAGAAAGTTTTCGGTGTTTGTCTAACGAAATTAAACATGTGCAT

mRNA sequence

ATGTCACTCCTTAAACTTGTGCAGCCTGATGTGACGGCGCCTGGTGTGAACGTTATTGCTGCTTACACGGAAGCCGTGAGCCCCACCGGCGAGCCCTTCGACAACAGAACGGCTCCATTCATCACCATGTCCGGCACCTCCATGTCCTGCCCCCACGTCGCCGGACTCGCCGGCCTCCTCCGATCTCTGCATCCTAATTGGAGCCCTTCCGCCATTAAATCCGCAATCATGACCTCTGCCAGAATCAGAGACAACACAATGAAGCCGATGCTCGACGGCGGCACAGACGAACTCGCTCCGGCCACTCCTTTCAGCTACGGCTCCGGCCACATTCGCCCAATCGGAGCCGTCGATCCAGGCCTGGTTTACGATCTGACGCCCGACGATTACTTGGAATTTCTGTGCGCCATCGGATACGATGAGAAGAAGATCAGAGCATTCTCCGACGGTCCGTACAAATGTCCGCCTTCCGCCAGTCTTCTGAACTTCAATTACCCTTCGATCGGAGTTCAGAACATGACCGGAAGCGTTACGGTGACCAGAAGATTGAAGAACGTGGGCACTCCCGGAGTTTACAGAGCCAGAGTTCGGCAGCCGGAAGGAGTTAGGGTTTCGGTGGAGCCGAGATTTCTGAAATTCGATAAAGTTGGAGAGGAGAAGAGCTTCAAATTGACGATCGCCGGAGTTGTGCCGGCCAAACGCGTGGTCGACGGGACGCTGATTTGGACCGACGGCGAGCACTTCGTTAGGAGTCCTATCGTCATTTCTTCCGGCTTGTTCTGA

Coding sequence (CDS)

ATGTCACTCCTTAAACTTGTGCAGCCTGATGTGACGGCGCCTGGTGTGAACGTTATTGCTGCTTACACGGAAGCCGTGAGCCCCACCGGCGAGCCCTTCGACAACAGAACGGCTCCATTCATCACCATGTCCGGCACCTCCATGTCCTGCCCCCACGTCGCCGGACTCGCCGGCCTCCTCCGATCTCTGCATCCTAATTGGAGCCCTTCCGCCATTAAATCCGCAATCATGACCTCTGCCAGAATCAGAGACAACACAATGAAGCCGATGCTCGACGGCGGCACAGACGAACTCGCTCCGGCCACTCCTTTCAGCTACGGCTCCGGCCACATTCGCCCAATCGGAGCCGTCGATCCAGGCCTGGTTTACGATCTGACGCCCGACGATTACTTGGAATTTCTGTGCGCCATCGGATACGATGAGAAGAAGATCAGAGCATTCTCCGACGGTCCGTACAAATGTCCGCCTTCCGCCAGTCTTCTGAACTTCAATTACCCTTCGATCGGAGTTCAGAACATGACCGGAAGCGTTACGGTGACCAGAAGATTGAAGAACGTGGGCACTCCCGGAGTTTACAGAGCCAGAGTTCGGCAGCCGGAAGGAGTTAGGGTTTCGGTGGAGCCGAGATTTCTGAAATTCGATAAAGTTGGAGAGGAGAAGAGCTTCAAATTGACGATCGCCGGAGTTGTGCCGGCCAAACGCGTGGTCGACGGGACGCTGATTTGGACCGACGGCGAGCACTTCGTTAGGAGTCCTATCGTCATTTCTTCCGGCTTGTTCTGA

Protein sequence

MSLLKLVQPDVTAPGVNVIAAYTEAVSPTGEPFDNRTAPFITMSGTSMSCPHVAGLAGLLRSLHPNWSPSAIKSAIMTSARIRDNTMKPMLDGGTDELAPATPFSYGSGHIRPIGAVDPGLVYDLTPDDYLEFLCAIGYDEKKIRAFSDGPYKCPPSASLLNFNYPSIGVQNMTGSVTVTRRLKNVGTPGVYRARVRQPEGVRVSVEPRFLKFDKVGEEKSFKLTIAGVVPAKRVVDGTLIWTDGEHFVRSPIVISSGLF

Homology

[Close]

BLAST of MC03g_new0253 vs. ExPASy Swiss-Prot
Match: F4JXC5 (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 SV=1)

HSP 1 Score: 302.0 bits (772), Expect = 6.6e-81
Identity = 146/252 (57.94%), Postives = 188/252 (74.60%), Query Frame = 0


Query: 6   LVQPDVTAPGVNVIAAYTEAVSPTGEPFDNRTAPFITMSGTSMSCPHVAGLAGLLRSLHP 65
           +++PD+TAPGVN+IAA+TEA  PT    DNR  PF T SGTSMSCPH++G+ GLL++LHP
Sbjct: 526 ILKPDITAPGVNIIAAFTEATGPTDLDSDNRRTPFNTESGTSMSCPHISGVVGLLKTLHP 585

Query: 66  NWSPSAIKSAIMTSARIRDNTMKPMLDGGTDELAPATPFSYGSGHIRPIGAVDPGLVYDL 125
           +WSP+AI+SAIMT++R R+N  KPM+D   +    A PFSYGSGH++P  A  PGLVYDL
Sbjct: 586 HWSPAAIRSAIMTTSRTRNNRRKPMVD---ESFKKANPFSYGSGHVQPNKAAHPGLVYDL 645

Query: 126 TPDDYLEFLCAIGYDEKKIRAFSDGP-YKCPPSASLLNFNYPSIGVQNMTGSVTVTRRLK 185
           T  DYL+FLCA+GY+   ++ F++ P Y C   A+LL+FNYPSI V N+TGS+TVTR+LK
Sbjct: 646 TTGDYLDFLCAVGYNNTVVQLFAEDPQYTCRQGANLLDFNYPSITVPNLTGSITVTRKLK 705

Query: 186 NVGTPGVYRARVRQPEGVRVSVEPRFLKFDKVGEEKSFKLTIAGV-VPAKRVVDGTLIWT 245
           NVG P  Y AR R+P GVRVSVEP+ L F+K GE K F++T+  + V     V G L WT
Sbjct: 706 NVGPPATYNARFREPLGVRVSVEPKQLTFNKTGEVKIFQMTLRPLPVTPSGYVFGELTWT 765

Query: 246 DGEHFVRSPIVI 256
           D  H+VRSPIV+
Sbjct: 766 DSHHYVRSPIVV 774

[Close]

BLAST of MC03g_new0253 vs. ExPASy Swiss-Prot
Match: Q9ZSP5 (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=1)

HSP 1 Score: 262.3 bits (669), Expect = 5.8e-69
Identity = 137/256 (53.52%), Postives = 184/256 (71.88%), Query Frame = 0


Query: 5   KLVQPDVTAPGVNVIAAYTEAVSPTGEPFDNRTAPFITMSGTSMSCPHVAGLAGLLRSLH 64
           ++++PD+TAPGV+VIAAYT AVSPT E FD R   F  +SGTSMSCPH++G+AGLL++ +
Sbjct: 519 QILKPDITAPGVSVIAAYTGAVSPTNEQFDPRRLLFNAISGTSMSCPHISGIAGLLKTRY 578

Query: 65  PNWSPSAIKSAIMTSARIRDNTMKPMLDGGTDELAPATPFSYGSGHIRPIGAVDPGLVYD 124
           P+WSP+AI+SAIMT+A I D+   P+ +        ATPFS+G+GH++P  AV+PGLVYD
Sbjct: 579 PSWSPAAIRSAIMTTATIMDDIPGPIQNATN---MKATPFSFGAGHVQPNLAVNPGLVYD 638

Query: 125 LTPDDYLEFLCAIGYDEKKIRAFSDGPYKC-PPSASLLNFNYPSIGVQNMTGS-VTVTRR 184
           L   DYL FLC++GY+  +I  FS   + C  P  SL+N NYPSI V N+T S VTV+R 
Sbjct: 639 LGIKDYLNFLCSLGYNASQISVFSGNNFTCSSPKISLVNLNYPSITVPNLTSSKVTVSRT 698

Query: 185 LKNVGTPGVYRARVRQPEGVRVSVEPRFLKFDKVGEEKSFKLTIA---GVVPAKRVVDGT 244
           +KNVG P +Y  +V  P+GV V+V+P  L F KVGE+K+FK+ +    G V AK  V G 
Sbjct: 699 VKNVGRPSMYTVKVNNPQGVYVAVKPTSLNFTKVGEQKTFKVILVKSKGNV-AKGYVFGE 758

Query: 245 LIWTDGEHFVRSPIVI 256
           L+W+D +H VRSPIV+
Sbjct: 759 LVWSDKKHRVRSPIVV 770

[Close]

BLAST of MC03g_new0253 vs. ExPASy Swiss-Prot
Match: I1N462 (Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 PE=1 SV=3)

HSP 1 Score: 235.3 bits (599), Expect = 7.6e-61
Identity = 123/255 (48.24%), Postives = 167/255 (65.49%), Query Frame = 0


Query: 6   LVQPDVTAPGVNVIAAYTEAVSPTGEPFDNRTA-PFITMSGTSMSCPHVAGLAGLLRSLH 65
           +++PDVTAPGVN++AAY+E  S +    DNR    F  + GTSMSCPH +G+AGLL++ H
Sbjct: 534 ILKPDVTAPGVNILAAYSEFASASSLLVDNRRGFKFNVLQGTSMSCPHASGIAGLLKTRH 593

Query: 66  PNWSPSAIKSAIMTSARIRDNTMKPMLDGGTDELAPATPFSYGSGHIRPIGAVDPGLVYD 125
           P+WSP+AIKSAIMT+A   DNT +P+ D     LA A  F+YGSGH+RP  A++PGLVYD
Sbjct: 594 PSWSPAAIKSAIMTTATTLDNTNRPIQDAFDKTLADA--FAYGSGHVRPDLAIEPGLVYD 653

Query: 126 LTPDDYLEFLCAIGYDEKKIRAFS-DGPYKCPPSASLLNFNYPSIGVQNM-TGSVTVTRR 185
           L+  DYL FLCA GYD++ I A + +  + C  S S+ + NYPSI + N+    VT+ R 
Sbjct: 654 LSLTDYLNFLCASGYDQQLISALNFNRTFICSGSHSVNDLNYPSITLPNLRLKPVTIART 713

Query: 186 LKNVGTPGVYRARVRQPEGVRVSVEPRFLKFDKVGEEKSFKLTI--AGVVPAKRVVDGTL 245
           + NVG P  Y    R P G  ++V P  L F K+GE K+FK+ +  +     ++   G L
Sbjct: 714 VTNVGPPSTYTVSTRSPNGYSIAVVPPSLTFTKIGERKTFKVIVQASSAATRRKYEFGDL 773

Query: 246 IWTDGEHFVRSPIVI 256
            WTDG+H VRSPI +
Sbjct: 774 RWTDGKHIVRSPITV 786

[Close]

BLAST of MC03g_new0253 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 225.7 bits (574), Expect = 6.0e-58
Identity = 118/257 (45.91%), Postives = 167/257 (64.98%), Query Frame = 0


Query: 6   LVQPDVTAPGVNVIAAYTEAVSPTGEPFDNRTAPFITMSGTSMSCPHVAGLAGLLRSLHP 65
           +++PD+ APGVN++AA+T A  PTG   D+R   F  +SGTSMSCPHV+GLA LL+S+HP
Sbjct: 501 ILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVHP 560

Query: 66  NWSPSAIKSAIMTSARIRDNTMKPMLDGGTDELAPATPFSYGSGHIRPIGAVDPGLVYDL 125
            WSP+AI+SA+MT+A       KP+LD  T +  P+TPF +G+GH+ P  A +PGL+YDL
Sbjct: 561 EWSPAAIRSALMTTAYKTYKDGKPLLDIATGK--PSTPFDHGAGHVSPTTATNPGLIYDL 620

Query: 126 TPDDYLEFLCAIGYDEKKIRAFSDGPYKCPPSA--SLLNFNYPSIGVQ-NMTGSVTVTRR 185
           T +DYL FLCA+ Y   +IR+ S   Y C PS   S+ + NYPS  V  +  G+   TR 
Sbjct: 621 TTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNYPSFAVNVDGVGAYKYTRT 680

Query: 186 LKNVGTPGVYRARV-RQPEGVRVSVEPRFLKFDKVGEEKSFKL--TIAGVVPAKRVVDGT 245
           + +VG  G Y  +V  +  GV++SVEP  L F +  E+KS+ +  T+    P+     G+
Sbjct: 681 VTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTVTFTVDSSKPSGSNSFGS 740

Query: 246 LIWTDGEHFVRSPIVIS 257
           + W+DG+H V SP+ IS
Sbjct: 741 IEWSDGKHVVGSPVAIS 755

[Close]

BLAST of MC03g_new0253 vs. ExPASy Swiss-Prot
Match: O49607 (Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 SV=1)

HSP 1 Score: 221.1 bits (562), Expect = 1.5e-56
Identity = 121/266 (45.49%), Postives = 170/266 (63.91%), Query Frame = 0


Query: 5   KLVQPDVTAPGVNVIAAYTEAVSPTGEPFDNRTAPFITMSGTSMSCPHVAGLAGLLRSLH 64
           ++++PD+ APGVN++AA+T+AV PTG P D R   F  +SGTSM+CPHV+G A LL+S H
Sbjct: 496 EILKPDLIAPGVNILAAWTDAVGPTGLPSDPRKTEFNILSGTSMACPHVSGAAALLKSAH 555

Query: 65  PNWSPSAIKSAIMTSARIRDNTMKPMLDGGTDELAPATPFSYGSGHIRPIGAVDPGLVYD 124
           P+WSP+ I+SA+MT+  + DN+ + ++D  T +   ATP+ YGSGH+    A++PGLVYD
Sbjct: 556 PDWSPAVIRSAMMTTTNLVDNSNRSLIDESTGK--SATPYDYGSGHLNLGRAMNPGLVYD 615

Query: 125 LTPDDYLEFLCAIGYDEKKIRAFSDGPYKCPPS--ASLLNFNYPSIGV---QNMTG--SV 184
           +T DDY+ FLC+IGY  K I+  +  P +CP +   S  N NYPSI      N  G  S 
Sbjct: 616 ITNDDYITFLCSIGYGPKTIQVITRTPVRCPTTRKPSPGNLNYPSITAVFPTNRRGLVSK 675

Query: 185 TVTRRLKNVG-TPGVYRARVRQPEGVRVSVEPRFLKFDKVGEEKSFKLTIA-----GVVP 244
           TV R   NVG    VYRAR+  P GV V+V+P  L F    + +S+ +T+       V+ 
Sbjct: 676 TVIRTATNVGQAEAVYRARIESPRGVTVTVKPPRLVFTSAVKRRSYAVTVTVNTRNVVLG 735

Query: 245 AKRVVDGTLIWTD-GEHFVRSPIVIS 257
               V G++ W D G+H VRSPIV++
Sbjct: 736 ETGAVFGSVTWFDGGKHVVRSPIVVT 759

[Close]

BLAST of MC03g_new0253 vs. NCBI nr
Match: XP_022142562.1 (subtilisin-like protease SBT5.4 [Momordica charantia])

HSP 1 Score: 518 bits (1334), Expect = 1.29e-177
Identity = 252/256 (98.44%), Postives = 256/256 (100.00%), Query Frame = 0


Query: 5   KLVQPDVTAPGVNVIAAYTEAVSPTGEPFDNRTAPFITMSGTSMSCPHVAGLAGLLRSLH 64
           ++++PDVTAPGVNVIAAYTEAVSPTGEPFDNRTAPFITMSGTSMSCPHVAGLAGLLRSLH
Sbjct: 524 EIIKPDVTAPGVNVIAAYTEAVSPTGEPFDNRTAPFITMSGTSMSCPHVAGLAGLLRSLH 583

Query: 65  PNWSPSAIKSAIMTSARIRDNTMKPMLDGGTDELAPATPFSYGSGHIRPIGAVDPGLVYD 124
           PNWSPSAIKSAIMTSARIRDNTMKPMLDGGTDELAPATPFSYGSGHIRPIGAVDPGLVYD
Sbjct: 584 PNWSPSAIKSAIMTSARIRDNTMKPMLDGGTDELAPATPFSYGSGHIRPIGAVDPGLVYD 643

Query: 125 LTPDDYLEFLCAIGYDEKKIRAFSDGPYKCPPSASLLNFNYPSIGVQNMTGSVTVTRRLK 184
           LTPDDYLEFLCAIGYDEKKIRAFSDGPYKCPPSASLLNFNYPSIGVQNMTGSVTVTRRLK
Sbjct: 644 LTPDDYLEFLCAIGYDEKKIRAFSDGPYKCPPSASLLNFNYPSIGVQNMTGSVTVTRRLK 703

Query: 185 NVGTPGVYRARVRQPEGVRVSVEPRFLKFDKVGEEKSFKLTIAGVVPAKRVVDGTLIWTD 244
           NVGTPGVYRARVRQPEGVRVSVEPRFLKFDKVGEEKSFKLTIAGVVPAKRVVDGTLIWTD
Sbjct: 704 NVGTPGVYRARVRQPEGVRVSVEPRFLKFDKVGEEKSFKLTIAGVVPAKRVVDGTLIWTD 763

Query: 245 GEHFVRSPIVISSGLF 260
           GEHFVRSPIVISSGLF
Sbjct: 764 GEHFVRSPIVISSGLF 779

[Close]

BLAST of MC03g_new0253 vs. NCBI nr
Match: XP_038895602.1 (subtilisin-like protease SBT5.4 [Benincasa hispida])

HSP 1 Score: 438 bits (1127), Expect = 1.71e-146
Identity = 204/256 (79.69%), Postives = 237/256 (92.58%), Query Frame = 0


Query: 5   KLVQPDVTAPGVNVIAAYTEAVSPTGEPFDNRTAPFITMSGTSMSCPHVAGLAGLLRSLH 64
           ++++PDVTAPGVNVIAA++EAVSPTGE FDNRT PFITMSGTSMSCPHV+GL GLLR+LH
Sbjct: 518 EIIKPDVTAPGVNVIAAFSEAVSPTGEAFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLH 577

Query: 65  PNWSPSAIKSAIMTSARIRDNTMKPMLDGGTDELAPATPFSYGSGHIRPIGAVDPGLVYD 124
           P+WSPSAIKSAIMTSARIRDNTMKPMLDGG+ +LAPATPF+YGSGHIRP GA+DPGLVYD
Sbjct: 578 PHWSPSAIKSAIMTSARIRDNTMKPMLDGGSPDLAPATPFAYGSGHIRPTGAIDPGLVYD 637

Query: 125 LTPDDYLEFLCAIGYDEKKIRAFSDGPYKCPPSASLLNFNYPSIGVQNMTGSVTVTRRLK 184
           L+P+DYLEFLCA GYD+K IRAFSDGP+KCPP+AS+LNFNYPSIGVQN+ G+VTVTR+LK
Sbjct: 638 LSPNDYLEFLCASGYDDKTIRAFSDGPFKCPPNASILNFNYPSIGVQNLNGNVTVTRKLK 697

Query: 185 NVGTPGVYRARVRQPEGVRVSVEPRFLKFDKVGEEKSFKLTIAGVVPAKRVVDGTLIWTD 244
           NV TPGVYRAR+  P+GV+VSV+P+ LKF++V EEKSF+LT+ G VP  +VVDG LIWTD
Sbjct: 698 NVSTPGVYRARIMNPDGVKVSVKPKVLKFERVAEEKSFELTMTGNVPEDQVVDGVLIWTD 757

Query: 245 GEHFVRSPIVISSGLF 260
           G+HFVRSPIVISS LF
Sbjct: 758 GKHFVRSPIVISSVLF 773

[Close]

BLAST of MC03g_new0253 vs. NCBI nr
Match: KAE8652826.1 (hypothetical protein Csa_022873 [Cucumis sativus])

HSP 1 Score: 435 bits (1118), Expect = 2.20e-145
Identity = 202/256 (78.91%), Postives = 234/256 (91.41%), Query Frame = 0


Query: 5   KLVQPDVTAPGVNVIAAYTEAVSPTGEPFDNRTAPFITMSGTSMSCPHVAGLAGLLRSLH 64
           ++++PDVTAPGVN+IAA++EAVSPTGEPFDNRT PFITMSGTSMSCPHV+GL GLLR+LH
Sbjct: 498 EIIKPDVTAPGVNIIAAFSEAVSPTGEPFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLH 557

Query: 65  PNWSPSAIKSAIMTSARIRDNTMKPMLDGGTDELAPATPFSYGSGHIRPIGAVDPGLVYD 124
           P WSPSAIKSAIMTSARIRDNT KPMLDGG+ +LAP+TPF+YGSGHIRP GA+DPGLVYD
Sbjct: 558 PQWSPSAIKSAIMTSARIRDNTKKPMLDGGSPDLAPSTPFAYGSGHIRPTGAIDPGLVYD 617

Query: 125 LTPDDYLEFLCAIGYDEKKIRAFSDGPYKCPPSASLLNFNYPSIGVQNMTGSVTVTRRLK 184
           L+P+DYLEFLCA GY+EK I+AFSDGP+KCP SAS+LN NYPSIGVQN+TGSVTVTR+LK
Sbjct: 618 LSPNDYLEFLCASGYNEKTIQAFSDGPFKCPASASILNLNYPSIGVQNLTGSVTVTRKLK 677

Query: 185 NVGTPGVYRARVRQPEGVRVSVEPRFLKFDKVGEEKSFKLTIAGVVPAKRVVDGTLIWTD 244
           NV TPGVY+ RVR P GV+V V+P+ LKF++VGEEKSF+LTI G VP  +VVDG LIWTD
Sbjct: 678 NVSTPGVYKGRVRHPNGVKVLVKPKVLKFERVGEEKSFELTITGDVPEDQVVDGVLIWTD 737

Query: 245 GEHFVRSPIVISSGLF 260
           G+HFVRSPIV+SS LF
Sbjct: 738 GKHFVRSPIVVSSSLF 753

[Close]

BLAST of MC03g_new0253 vs. NCBI nr
Match: XP_031739111.1 (subtilisin-like protease SBT5.4 [Cucumis sativus])

HSP 1 Score: 435 bits (1118), Expect = 2.47e-145
Identity = 202/256 (78.91%), Postives = 234/256 (91.41%), Query Frame = 0


Query: 5   KLVQPDVTAPGVNVIAAYTEAVSPTGEPFDNRTAPFITMSGTSMSCPHVAGLAGLLRSLH 64
           ++++PDVTAPGVN+IAA++EAVSPTGEPFDNRT PFITMSGTSMSCPHV+GL GLLR+LH
Sbjct: 502 EIIKPDVTAPGVNIIAAFSEAVSPTGEPFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLH 561

Query: 65  PNWSPSAIKSAIMTSARIRDNTMKPMLDGGTDELAPATPFSYGSGHIRPIGAVDPGLVYD 124
           P WSPSAIKSAIMTSARIRDNT KPMLDGG+ +LAP+TPF+YGSGHIRP GA+DPGLVYD
Sbjct: 562 PQWSPSAIKSAIMTSARIRDNTKKPMLDGGSPDLAPSTPFAYGSGHIRPTGAIDPGLVYD 621

Query: 125 LTPDDYLEFLCAIGYDEKKIRAFSDGPYKCPPSASLLNFNYPSIGVQNMTGSVTVTRRLK 184
           L+P+DYLEFLCA GY+EK I+AFSDGP+KCP SAS+LN NYPSIGVQN+TGSVTVTR+LK
Sbjct: 622 LSPNDYLEFLCASGYNEKTIQAFSDGPFKCPASASILNLNYPSIGVQNLTGSVTVTRKLK 681

Query: 185 NVGTPGVYRARVRQPEGVRVSVEPRFLKFDKVGEEKSFKLTIAGVVPAKRVVDGTLIWTD 244
           NV TPGVY+ RVR P GV+V V+P+ LKF++VGEEKSF+LTI G VP  +VVDG LIWTD
Sbjct: 682 NVSTPGVYKGRVRHPNGVKVLVKPKVLKFERVGEEKSFELTITGDVPEDQVVDGVLIWTD 741

Query: 245 GEHFVRSPIVISSGLF 260
           G+HFVRSPIV+SS LF
Sbjct: 742 GKHFVRSPIVVSSSLF 757

[Close]

BLAST of MC03g_new0253 vs. NCBI nr
Match: XP_008464322.1 (PREDICTED: subtilisin-like protease SBT5.4 [Cucumis melo])

HSP 1 Score: 435 bits (1119), Expect = 2.97e-145
Identity = 202/256 (78.91%), Postives = 235/256 (91.80%), Query Frame = 0


Query: 5   KLVQPDVTAPGVNVIAAYTEAVSPTGEPFDNRTAPFITMSGTSMSCPHVAGLAGLLRSLH 64
           ++++PDVTAPGVN+IAA++EAVSPTGEPFDNRT PFITMSGTSMSCPHV+GL GLLR+LH
Sbjct: 521 EIIKPDVTAPGVNIIAAFSEAVSPTGEPFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLH 580

Query: 65  PNWSPSAIKSAIMTSARIRDNTMKPMLDGGTDELAPATPFSYGSGHIRPIGAVDPGLVYD 124
           P+WSPSAIKSAIMTSARIRDNT KPMLDGG+ +LAPATPF+YGSGHIRP GA+DPGLVYD
Sbjct: 581 PHWSPSAIKSAIMTSARIRDNTKKPMLDGGSPDLAPATPFAYGSGHIRPTGAIDPGLVYD 640

Query: 125 LTPDDYLEFLCAIGYDEKKIRAFSDGPYKCPPSASLLNFNYPSIGVQNMTGSVTVTRRLK 184
           L+P+DYLEFLCA GY+EK I+AFSDGP+KCP SAS+LNFNYPSIGVQN+TGSVT+TR+LK
Sbjct: 641 LSPNDYLEFLCASGYNEKTIQAFSDGPFKCPASASILNFNYPSIGVQNLTGSVTLTRKLK 700

Query: 185 NVGTPGVYRARVRQPEGVRVSVEPRFLKFDKVGEEKSFKLTIAGVVPAKRVVDGTLIWTD 244
           NV TPGVY+ARV  P GV+V V+P+ LKF++VGEEK F+L I G VP  +VVDG LIWTD
Sbjct: 701 NVSTPGVYKARVMHPNGVKVLVKPKVLKFERVGEEKRFELIITGDVPENQVVDGVLIWTD 760

Query: 245 GEHFVRSPIVISSGLF 260
           G+HFVRSPIV+SSGLF
Sbjct: 761 GKHFVRSPIVVSSGLF 776

[Close]

BLAST of MC03g_new0253 vs. ExPASy TrEMBL
Match: A0A6J1CN43 (subtilisin-like protease SBT5.4 OS=Momordica charantia OX=3673 GN=LOC111012645 PE=3 SV=1)

HSP 1 Score: 518 bits (1334), Expect = 6.25e-178
Identity = 252/256 (98.44%), Postives = 256/256 (100.00%), Query Frame = 0


Query: 5   KLVQPDVTAPGVNVIAAYTEAVSPTGEPFDNRTAPFITMSGTSMSCPHVAGLAGLLRSLH 64
           ++++PDVTAPGVNVIAAYTEAVSPTGEPFDNRTAPFITMSGTSMSCPHVAGLAGLLRSLH
Sbjct: 524 EIIKPDVTAPGVNVIAAYTEAVSPTGEPFDNRTAPFITMSGTSMSCPHVAGLAGLLRSLH 583

Query: 65  PNWSPSAIKSAIMTSARIRDNTMKPMLDGGTDELAPATPFSYGSGHIRPIGAVDPGLVYD 124
           PNWSPSAIKSAIMTSARIRDNTMKPMLDGGTDELAPATPFSYGSGHIRPIGAVDPGLVYD
Sbjct: 584 PNWSPSAIKSAIMTSARIRDNTMKPMLDGGTDELAPATPFSYGSGHIRPIGAVDPGLVYD 643

Query: 125 LTPDDYLEFLCAIGYDEKKIRAFSDGPYKCPPSASLLNFNYPSIGVQNMTGSVTVTRRLK 184
           LTPDDYLEFLCAIGYDEKKIRAFSDGPYKCPPSASLLNFNYPSIGVQNMTGSVTVTRRLK
Sbjct: 644 LTPDDYLEFLCAIGYDEKKIRAFSDGPYKCPPSASLLNFNYPSIGVQNMTGSVTVTRRLK 703

Query: 185 NVGTPGVYRARVRQPEGVRVSVEPRFLKFDKVGEEKSFKLTIAGVVPAKRVVDGTLIWTD 244
           NVGTPGVYRARVRQPEGVRVSVEPRFLKFDKVGEEKSFKLTIAGVVPAKRVVDGTLIWTD
Sbjct: 704 NVGTPGVYRARVRQPEGVRVSVEPRFLKFDKVGEEKSFKLTIAGVVPAKRVVDGTLIWTD 763

Query: 245 GEHFVRSPIVISSGLF 260
           GEHFVRSPIVISSGLF
Sbjct: 764 GEHFVRSPIVISSGLF 779

[Close]

BLAST of MC03g_new0253 vs. ExPASy TrEMBL
Match: A0A0A0LVY8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G171040 PE=3 SV=1)

HSP 1 Score: 435 bits (1118), Expect = 1.19e-145
Identity = 202/256 (78.91%), Postives = 234/256 (91.41%), Query Frame = 0


Query: 5   KLVQPDVTAPGVNVIAAYTEAVSPTGEPFDNRTAPFITMSGTSMSCPHVAGLAGLLRSLH 64
           ++++PDVTAPGVN+IAA++EAVSPTGEPFDNRT PFITMSGTSMSCPHV+GL GLLR+LH
Sbjct: 502 EIIKPDVTAPGVNIIAAFSEAVSPTGEPFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLH 561

Query: 65  PNWSPSAIKSAIMTSARIRDNTMKPMLDGGTDELAPATPFSYGSGHIRPIGAVDPGLVYD 124
           P WSPSAIKSAIMTSARIRDNT KPMLDGG+ +LAP+TPF+YGSGHIRP GA+DPGLVYD
Sbjct: 562 PQWSPSAIKSAIMTSARIRDNTKKPMLDGGSPDLAPSTPFAYGSGHIRPTGAIDPGLVYD 621

Query: 125 LTPDDYLEFLCAIGYDEKKIRAFSDGPYKCPPSASLLNFNYPSIGVQNMTGSVTVTRRLK 184
           L+P+DYLEFLCA GY+EK I+AFSDGP+KCP SAS+LN NYPSIGVQN+TGSVTVTR+LK
Sbjct: 622 LSPNDYLEFLCASGYNEKTIQAFSDGPFKCPASASILNLNYPSIGVQNLTGSVTVTRKLK 681

Query: 185 NVGTPGVYRARVRQPEGVRVSVEPRFLKFDKVGEEKSFKLTIAGVVPAKRVVDGTLIWTD 244
           NV TPGVY+ RVR P GV+V V+P+ LKF++VGEEKSF+LTI G VP  +VVDG LIWTD
Sbjct: 682 NVSTPGVYKGRVRHPNGVKVLVKPKVLKFERVGEEKSFELTITGDVPEDQVVDGVLIWTD 741

Query: 245 GEHFVRSPIVISSGLF 260
           G+HFVRSPIV+SS LF
Sbjct: 742 GKHFVRSPIVVSSSLF 757

[Close]

BLAST of MC03g_new0253 vs. ExPASy TrEMBL
Match: A0A1S3CLB7 (subtilisin-like protease SBT5.4 OS=Cucumis melo OX=3656 GN=LOC103502236 PE=3 SV=1)

HSP 1 Score: 435 bits (1119), Expect = 1.44e-145
Identity = 202/256 (78.91%), Postives = 235/256 (91.80%), Query Frame = 0


Query: 5   KLVQPDVTAPGVNVIAAYTEAVSPTGEPFDNRTAPFITMSGTSMSCPHVAGLAGLLRSLH 64
           ++++PDVTAPGVN+IAA++EAVSPTGEPFDNRT PFITMSGTSMSCPHV+GL GLLR+LH
Sbjct: 521 EIIKPDVTAPGVNIIAAFSEAVSPTGEPFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLH 580

Query: 65  PNWSPSAIKSAIMTSARIRDNTMKPMLDGGTDELAPATPFSYGSGHIRPIGAVDPGLVYD 124
           P+WSPSAIKSAIMTSARIRDNT KPMLDGG+ +LAPATPF+YGSGHIRP GA+DPGLVYD
Sbjct: 581 PHWSPSAIKSAIMTSARIRDNTKKPMLDGGSPDLAPATPFAYGSGHIRPTGAIDPGLVYD 640

Query: 125 LTPDDYLEFLCAIGYDEKKIRAFSDGPYKCPPSASLLNFNYPSIGVQNMTGSVTVTRRLK 184
           L+P+DYLEFLCA GY+EK I+AFSDGP+KCP SAS+LNFNYPSIGVQN+TGSVT+TR+LK
Sbjct: 641 LSPNDYLEFLCASGYNEKTIQAFSDGPFKCPASASILNFNYPSIGVQNLTGSVTLTRKLK 700

Query: 185 NVGTPGVYRARVRQPEGVRVSVEPRFLKFDKVGEEKSFKLTIAGVVPAKRVVDGTLIWTD 244
           NV TPGVY+ARV  P GV+V V+P+ LKF++VGEEK F+L I G VP  +VVDG LIWTD
Sbjct: 701 NVSTPGVYKARVMHPNGVKVLVKPKVLKFERVGEEKRFELIITGDVPENQVVDGVLIWTD 760

Query: 245 GEHFVRSPIVISSGLF 260
           G+HFVRSPIV+SSGLF
Sbjct: 761 GKHFVRSPIVVSSGLF 776

[Close]

BLAST of MC03g_new0253 vs. ExPASy TrEMBL
Match: A0A6J1F4N6 (subtilisin-like protease SBT5.4 OS=Cucurbita moschata OX=3662 GN=LOC111440718 PE=3 SV=1)

HSP 1 Score: 400 bits (1027), Expect = 6.35e-139
Identity = 186/252 (73.81%), Postives = 217/252 (86.11%), Query Frame = 0


Query: 9   PDVTAPGVNVIAAYTEAVSPTGEPFDNRTAPFITMSGTSMSCPHVAGLAGLLRSLHPNWS 68
           PDVTAPGVN+IAA++ AVSPTGEPFDNR  P+ITMSGTSMSCPHV+G+ GLL++LHP WS
Sbjct: 1   PDVTAPGVNIIAAFSGAVSPTGEPFDNRAVPYITMSGTSMSCPHVSGIVGLLKALHPEWS 60

Query: 69  PSAIKSAIMTSARIRDNTMKPMLDGGTDELAPATPFSYGSGHIRPIGAVDPGLVYDLTPD 128
           P+AIKSAIMTSAR+ DNTM  MLDGG+   APATPF YGSGHIRP GA+DPGLVYDL+P+
Sbjct: 61  PAAIKSAIMTSARVSDNTMNLMLDGGSPIFAPATPFVYGSGHIRPTGAIDPGLVYDLSPN 120

Query: 129 DYLEFLCAIGYDEKKIRAFSDGPYKCPPSASLLNFNYPSIGVQNMTGSVTVTRRLKNVGT 188
           DYLEFLCA GY EK +R F+DG +KCP S S+LNFNYPSIGVQN+TGSVTV+RRLKNVG 
Sbjct: 121 DYLEFLCASGYTEKNLRVFTDGNFKCPVSGSILNFNYPSIGVQNLTGSVTVSRRLKNVGM 180

Query: 189 PGVYRARVRQPEGVRVSVEPRFLKFDKVGEEKSFKLTIAGVVPAKRVVDGTLIWTDGEHF 248
           PGVYR RV+QPEGV+VSV+P  LKF+K+GEEK F+LT+ G V   ++  GTLIWTD +HF
Sbjct: 181 PGVYRVRVQQPEGVKVSVKPNVLKFEKIGEEKGFELTMTGAVAEGQIGYGTLIWTDDKHF 240

Query: 249 VRSPIVISSGLF 260
           VRSPIV+SS  F
Sbjct: 241 VRSPIVVSSSFF 252

[Close]

BLAST of MC03g_new0253 vs. ExPASy TrEMBL
Match: A0A0A0LYF1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G171030 PE=3 SV=1)

HSP 1 Score: 410 bits (1055), Expect = 5.70e-136
Identity = 190/256 (74.22%), Postives = 228/256 (89.06%), Query Frame = 0


Query: 5   KLVQPDVTAPGVNVIAAYTEAVSPTGEPFDNRTAPFITMSGTSMSCPHVAGLAGLLRSLH 64
           ++++PDVTAPGV++IAA++EA+SPT +P DNRT PFITMSGTSMSCPHVAGL GLLR+LH
Sbjct: 520 EIIKPDVTAPGVDIIAAFSEAISPTRDPSDNRTTPFITMSGTSMSCPHVAGLVGLLRNLH 579

Query: 65  PNWSPSAIKSAIMTSARIRDNTMKPMLDGGTDELAPATPFSYGSGHIRPIGAVDPGLVYD 124
           P+W+PSAIKSAIMTSA++RDNT+ PMLDGG+  L PATPF+YGSGHI P GAVDPGLVYD
Sbjct: 580 PDWTPSAIKSAIMTSAQVRDNTLNPMLDGGSLGLDPATPFAYGSGHINPTGAVDPGLVYD 639

Query: 125 LTPDDYLEFLCAIGYDEKKIRAFSDGPYKCPPSASLLNFNYPSIGVQNMTGSVTVTRRLK 184
           L+P+DYLEFLCA GYDE+ IRAFSD P+KCP SAS+LN NYPSIGVQN+  SVT+TR+LK
Sbjct: 640 LSPNDYLEFLCASGYDERTIRAFSDEPFKCPASASVLNLNYPSIGVQNLKDSVTITRKLK 699

Query: 185 NVGTPGVYRARVRQPEGVRVSVEPRFLKFDKVGEEKSFKLTIAGVVPAKRVVDGTLIWTD 244
           NVGTPGVY+A++  P  V+VSV+PRFLKF++VGEEKSF+LT++GVVP  R   G LIW+D
Sbjct: 700 NVGTPGVYKAQILHPNVVQVSVKPRFLKFERVGEEKSFELTVSGVVPKNRFAYGALIWSD 759

Query: 245 GEHFVRSPIVISSGLF 260
           G HFVRSPIV+SSGLF
Sbjct: 760 GRHFVRSPIVVSSGLF 775

[Close]

BLAST of MC03g_new0253 vs. TAIR 10
Match: AT5G59810.1 (Subtilase family protein )

HSP 1 Score: 302.0 bits (772), Expect = 4.7e-82
Identity = 146/252 (57.94%), Postives = 188/252 (74.60%), Query Frame = 0


Query: 6   LVQPDVTAPGVNVIAAYTEAVSPTGEPFDNRTAPFITMSGTSMSCPHVAGLAGLLRSLHP 65
           +++PD+TAPGVN+IAA+TEA  PT    DNR  PF T SGTSMSCPH++G+ GLL++LHP
Sbjct: 526 ILKPDITAPGVNIIAAFTEATGPTDLDSDNRRTPFNTESGTSMSCPHISGVVGLLKTLHP 585

Query: 66  NWSPSAIKSAIMTSARIRDNTMKPMLDGGTDELAPATPFSYGSGHIRPIGAVDPGLVYDL 125
           +WSP+AI+SAIMT++R R+N  KPM+D   +    A PFSYGSGH++P  A  PGLVYDL
Sbjct: 586 HWSPAAIRSAIMTTSRTRNNRRKPMVD---ESFKKANPFSYGSGHVQPNKAAHPGLVYDL 645

Query: 126 TPDDYLEFLCAIGYDEKKIRAFSDGP-YKCPPSASLLNFNYPSIGVQNMTGSVTVTRRLK 185
           T  DYL+FLCA+GY+   ++ F++ P Y C   A+LL+FNYPSI V N+TGS+TVTR+LK
Sbjct: 646 TTGDYLDFLCAVGYNNTVVQLFAEDPQYTCRQGANLLDFNYPSITVPNLTGSITVTRKLK 705

Query: 186 NVGTPGVYRARVRQPEGVRVSVEPRFLKFDKVGEEKSFKLTIAGV-VPAKRVVDGTLIWT 245
           NVG P  Y AR R+P GVRVSVEP+ L F+K GE K F++T+  + V     V G L WT
Sbjct: 706 NVGPPATYNARFREPLGVRVSVEPKQLTFNKTGEVKIFQMTLRPLPVTPSGYVFGELTWT 765

Query: 246 DGEHFVRSPIVI 256
           D  H+VRSPIV+
Sbjct: 766 DSHHYVRSPIVV 774

[Close]

BLAST of MC03g_new0253 vs. TAIR 10
Match: AT2G04160.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 262.3 bits (669), Expect = 4.1e-70
Identity = 137/256 (53.52%), Postives = 184/256 (71.88%), Query Frame = 0


Query: 5   KLVQPDVTAPGVNVIAAYTEAVSPTGEPFDNRTAPFITMSGTSMSCPHVAGLAGLLRSLH 64
           ++++PD+TAPGV+VIAAYT AVSPT E FD R   F  +SGTSMSCPH++G+AGLL++ +
Sbjct: 519 QILKPDITAPGVSVIAAYTGAVSPTNEQFDPRRLLFNAISGTSMSCPHISGIAGLLKTRY 578

Query: 65  PNWSPSAIKSAIMTSARIRDNTMKPMLDGGTDELAPATPFSYGSGHIRPIGAVDPGLVYD 124
           P+WSP+AI+SAIMT+A I D+   P+ +        ATPFS+G+GH++P  AV+PGLVYD
Sbjct: 579 PSWSPAAIRSAIMTTATIMDDIPGPIQNATN---MKATPFSFGAGHVQPNLAVNPGLVYD 638

Query: 125 LTPDDYLEFLCAIGYDEKKIRAFSDGPYKC-PPSASLLNFNYPSIGVQNMTGS-VTVTRR 184
           L   DYL FLC++GY+  +I  FS   + C  P  SL+N NYPSI V N+T S VTV+R 
Sbjct: 639 LGIKDYLNFLCSLGYNASQISVFSGNNFTCSSPKISLVNLNYPSITVPNLTSSKVTVSRT 698

Query: 185 LKNVGTPGVYRARVRQPEGVRVSVEPRFLKFDKVGEEKSFKLTIA---GVVPAKRVVDGT 244
           +KNVG P +Y  +V  P+GV V+V+P  L F KVGE+K+FK+ +    G V AK  V G 
Sbjct: 699 VKNVGRPSMYTVKVNNPQGVYVAVKPTSLNFTKVGEQKTFKVILVKSKGNV-AKGYVFGE 758

Query: 245 LIWTDGEHFVRSPIVI 256
           L+W+D +H VRSPIV+
Sbjct: 759 LVWSDKKHRVRSPIVV 770

[Close]

BLAST of MC03g_new0253 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 225.7 bits (574), Expect = 4.3e-59
Identity = 118/257 (45.91%), Postives = 167/257 (64.98%), Query Frame = 0


Query: 6   LVQPDVTAPGVNVIAAYTEAVSPTGEPFDNRTAPFITMSGTSMSCPHVAGLAGLLRSLHP 65
           +++PD+ APGVN++AA+T A  PTG   D+R   F  +SGTSMSCPHV+GLA LL+S+HP
Sbjct: 501 ILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVHP 560

Query: 66  NWSPSAIKSAIMTSARIRDNTMKPMLDGGTDELAPATPFSYGSGHIRPIGAVDPGLVYDL 125
            WSP+AI+SA+MT+A       KP+LD  T +  P+TPF +G+GH+ P  A +PGL+YDL
Sbjct: 561 EWSPAAIRSALMTTAYKTYKDGKPLLDIATGK--PSTPFDHGAGHVSPTTATNPGLIYDL 620

Query: 126 TPDDYLEFLCAIGYDEKKIRAFSDGPYKCPPSA--SLLNFNYPSIGVQ-NMTGSVTVTRR 185
           T +DYL FLCA+ Y   +IR+ S   Y C PS   S+ + NYPS  V  +  G+   TR 
Sbjct: 621 TTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNYPSFAVNVDGVGAYKYTRT 680

Query: 186 LKNVGTPGVYRARV-RQPEGVRVSVEPRFLKFDKVGEEKSFKL--TIAGVVPAKRVVDGT 245
           + +VG  G Y  +V  +  GV++SVEP  L F +  E+KS+ +  T+    P+     G+
Sbjct: 681 VTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTVTFTVDSSKPSGSNSFGS 740

Query: 246 LIWTDGEHFVRSPIVIS 257
           + W+DG+H V SP+ IS
Sbjct: 741 IEWSDGKHVVGSPVAIS 755

[Close]

BLAST of MC03g_new0253 vs. TAIR 10
Match: AT4G34980.1 (subtilisin-like serine protease 2 )

HSP 1 Score: 221.1 bits (562), Expect = 1.0e-57
Identity = 121/266 (45.49%), Postives = 170/266 (63.91%), Query Frame = 0


Query: 5   KLVQPDVTAPGVNVIAAYTEAVSPTGEPFDNRTAPFITMSGTSMSCPHVAGLAGLLRSLH 64
           ++++PD+ APGVN++AA+T+AV PTG P D R   F  +SGTSM+CPHV+G A LL+S H
Sbjct: 496 EILKPDLIAPGVNILAAWTDAVGPTGLPSDPRKTEFNILSGTSMACPHVSGAAALLKSAH 555

Query: 65  PNWSPSAIKSAIMTSARIRDNTMKPMLDGGTDELAPATPFSYGSGHIRPIGAVDPGLVYD 124
           P+WSP+ I+SA+MT+  + DN+ + ++D  T +   ATP+ YGSGH+    A++PGLVYD
Sbjct: 556 PDWSPAVIRSAMMTTTNLVDNSNRSLIDESTGK--SATPYDYGSGHLNLGRAMNPGLVYD 615

Query: 125 LTPDDYLEFLCAIGYDEKKIRAFSDGPYKCPPS--ASLLNFNYPSIGV---QNMTG--SV 184
           +T DDY+ FLC+IGY  K I+  +  P +CP +   S  N NYPSI      N  G  S 
Sbjct: 616 ITNDDYITFLCSIGYGPKTIQVITRTPVRCPTTRKPSPGNLNYPSITAVFPTNRRGLVSK 675

Query: 185 TVTRRLKNVG-TPGVYRARVRQPEGVRVSVEPRFLKFDKVGEEKSFKLTIA-----GVVP 244
           TV R   NVG    VYRAR+  P GV V+V+P  L F    + +S+ +T+       V+ 
Sbjct: 676 TVIRTATNVGQAEAVYRARIESPRGVTVTVKPPRLVFTSAVKRRSYAVTVTVNTRNVVLG 735

Query: 245 AKRVVDGTLIWTD-GEHFVRSPIVIS 257
               V G++ W D G+H VRSPIV++
Sbjct: 736 ETGAVFGSVTWFDGGKHVVRSPIVVT 759

[Close]

BLAST of MC03g_new0253 vs. TAIR 10
Match: AT4G26330.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 205.3 bits (521), Expect = 6.0e-53
Identity = 112/259 (43.24%), Postives = 163/259 (62.93%), Query Frame = 0


Query: 6   LVQPDVTAPGVNVIAAYTEAVSPTGEPFDNRTAPFITMSGTSMSCPHVAGLAGLLRSLHP 65
           +++PD+TAPG+ ++AA+     PT  P D+R+  +   SGTSMSCPHVAG+  LL+S HP
Sbjct: 479 ILKPDITAPGIGILAAWPPRTPPTLLPGDHRSIEWNFQSGTSMSCPHVAGVMALLQSAHP 538

Query: 66  NWSPSAIKSAIMTSARIRDNTMKPMLDGGTDELAPATPFSYGSGHIRPIGAVDPGLVYDL 125
           +WSPSAI+SAIMT+A  RD +   +L GG+  +    PF  G+GHI P+ A+DPGLVY+ 
Sbjct: 539 DWSPSAIRSAIMTTAYTRDTSYDLILSGGS--MKSTDPFDIGAGHINPLKAMDPGLVYNT 598

Query: 126 TPDDYLEFLCAIGYDEKKIRAFSDGP---YKCPPSASL---LNFNYPSIGVQNMTGSVTV 185
             DDY+ F+C IGY +++I++    P     C PS S     +FNYPSI + ++  + T+
Sbjct: 599 RTDDYVLFMCNIGYTDQEIKSMVLHPEPSTTCLPSHSYRTNADFNYPSITIPSLRLTRTI 658

Query: 186 TRRLKNVG--TPGVYRARVRQPEGVRVSVEPRFLKFDKVGEEKSFKLTIAGV-VPAKRVV 245
            R + NVG     VY   + +P GV V + PR L F K  +E S+ +T     + + R V
Sbjct: 659 KRTVSNVGPNKNTVYFVDIIRPVGVEVLIWPRILVFSKCQQEHSYYVTFKPTEIFSGRYV 718

Query: 246 DGTLIWTDGEHFVRSPIVI 256
            G ++WT+G H VRSP+V+
Sbjct: 719 FGEIMWTNGLHRVRSPVVV 735

The following BLAST results are available for this feature:

BLAST of MC03g_new0253 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4JXC5	6.6e-81	57.94	Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 S...	[more]
Q9ZSP5	5.8e-69	53.52	Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=...	[more]
I1N462	7.6e-61	48.24	Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 P...	[more]
O65351	6.0e-58	45.91	Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 S...	[more]
O49607	1.5e-56	45.49	Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 S...	[more]

BLAST of MC03g_new0253 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022142562.1	1.29e-177	98.44	subtilisin-like protease SBT5.4 [Momordica charantia]	[more]
XP_038895602.1	1.71e-146	79.69	subtilisin-like protease SBT5.4 [Benincasa hispida]	[more]
KAE8652826.1	2.20e-145	78.91	hypothetical protein Csa_022873 [Cucumis sativus]	[more]
XP_031739111.1	2.47e-145	78.91	subtilisin-like protease SBT5.4 [Cucumis sativus]	[more]
XP_008464322.1	2.97e-145	78.91	PREDICTED: subtilisin-like protease SBT5.4 [Cucumis melo]	[more]

BLAST of MC03g_new0253 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CN43	6.25e-178	98.44	subtilisin-like protease SBT5.4 OS=Momordica charantia OX=3673 GN=LOC111012645 P...	[more]
A0A0A0LVY8	1.19e-145	78.91	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G171040 PE=3 SV=1	[more]
A0A1S3CLB7	1.44e-145	78.91	subtilisin-like protease SBT5.4 OS=Cucumis melo OX=3656 GN=LOC103502236 PE=3 SV=...	[more]
A0A6J1F4N6	6.35e-139	73.81	subtilisin-like protease SBT5.4 OS=Cucurbita moschata OX=3662 GN=LOC111440718 PE...	[more]
A0A0A0LYF1	5.70e-136	74.22	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G171030 PE=3 SV=1	[more]

BLAST of MC03g_new0253 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G59810.1	4.7e-82	57.94	Subtilase family protein	[more]
AT2G04160.1	4.1e-70	53.52	Subtilisin-like serine endopeptidase family protein	[more]
AT5G67360.1	4.3e-59	45.91	Subtilase family protein	[more]
AT4G34980.1	1.0e-57	45.49	subtilisin-like serine protease 2	[more]
AT4G26330.1	6.0e-53	43.24	Subtilisin-like serine endopeptidase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR041469	Subtilisin-like protease, fibronectin type-III domain	PFAM	PF17766	fn3_6	coord: 162..255 e-value: 6.2E-27 score: 93.7
None	No IPR available	GENE3D	2.60.40.2310		coord: 134..258 e-value: 2.1E-36 score: 126.5
None	No IPR available	PANTHER	PTHR43806:SF40	BNAC02G33520D PROTEIN	coord: 6..256
None	No IPR available	PANTHER	PTHR43806	PEPTIDASE S8	coord: 6..256
None	No IPR available	PROSITE	PS51892	SUBTILASE	coord: 1..117 score: 12.36021
IPR000209	Peptidase S8/S53 domain	PFAM	PF00082	Peptidase_S8	coord: 7..109 e-value: 5.1E-16 score: 58.8
IPR036852	Peptidase S8/S53 domain superfamily	GENE3D	3.40.50.200	Peptidase S8/S53 domain	coord: 6..130 e-value: 9.2E-45 score: 155.2
IPR036852	Peptidase S8/S53 domain superfamily	SUPERFAMILY	52743	Subtilisin-like	coord: 7..118
IPR023828	Peptidase S8, subtilisin, Ser-active site	PROSITE	PS00138	SUBTILASE_SER	coord: 45..55

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC03g_new0253.1	MC03g_new0253.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
biological_process	GO:0009610	response to symbiotic fungus
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004252	serine-type endopeptidase activity
molecular_function	GO:0008236	serine-type peptidase activity