MC03g1115 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC03g1115
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionprotein kinase 2B, chloroplastic-like
LocationMC03: 17370653 .. 17373586 (-)
RNA-Seq ExpressionMC03g1115
SyntenyMC03g1115
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
GAAGAACCAAAAAAATCTGAGATGAAAGACCATTCATCGGCTACTTTGAAAGCTCCATCACTTGGGGTGAATGTTCCATCTAGAAGTCTTCAGGCTCATAGATCTGAAGGTGAAATATTGTTGTCCCCCAATCTGAAAGCCTTCACATTCAGTGAGCTTAAGAATGCCACTAAAAACTTCCGCCCTGACAGTCTCATTGGAGAGGGAGGTTTCGGTTATGTGTACAAAGGATGGATTGATGAACATACACTTGAAGCTGTGAGGCCTGGAGCTGGAATGGTTATTGCTGTCAAGAAGCTTAAACCTGAAGGTTTTCAAGGGCACAAGGAATGGCTGGTAGTTTTCTCTTTCTTTCTGATGCGTCCCTTTATCTTTCTGGCTTAATAGTTTGCTTTATAAATCGGGTTGCTATTGATTGCAGTCTGAAGTAAATTATCTCGGTCAGCTTCATCATTCAAATCTTGTGAAACTAATTGGATTCTGCCTTGATGGGGATAGCCGACTTTTGGTCTACGAGTACATGTCCAAAGGCAGCCTTGAAAACCATCTATTTAGAAGTAAGTCTAATGTTTTGGCTAACTGTAACTGAAAGCCTGCTGCAACCATTAACCATTTTGTGGAAATGTTACTGTAATTAAGTAGGCATGATAAATCCATTAAGTAATTAATTAGGTTCTGAACCTCTGTTCTTGGATGTGCAATGACATTGAATTCTTTTCTTAACCATATCTATTGTCACAATTTTGCAGGGGGTGCCCGACCACTTTCTTGGGCAATAAGGATTAAAGTTGCTATAGGTGCTGCTCGAGGGCTTTCCTTTCTGCATAACTCTGAAGAACCAGTTATCTATCGCGATTTTAAGGCATCAAACATCCTTCTAGATTCTGTATGTCAATTAAGTCCTTCACATCAAAGACTTCTGTAAACTCTTTTAACTAGATCTTATGGAAAACAAAATAGATGCAAGTAAATAAAGTACTAATGGAAAAAGTGAAGTAATAGCAAGGAGAGAGGAGATAAAACACTTTTCCCCCGTTGTCCTTCCTTTTATTATTAAATACAAAGAATCAAGAATTTAGGTCCACAAGCACTGTATAATTAAAACTCGCCTGTCTGACTAAGTATGAAATTATTTTGTACGCAGGAATTCAACGCAAAACTTTCAGATTTTGGCTTGGCAAAAGCTGGCCCAACTGGTGATCGGACTCATGTGTCCACTCAAGTCATGGGTACTCAAGGCTATGCTGCTCCAGAATATATTGCCACTGGTTTGTTTTCTGCTCCATTTTGAGAATCTTCGTTTTTCTGTCAGTAATTAGTAGAACAAACGTGAATTTCATGTTTTCCAACCATGAACGACATAGGAGAAGCATCCCTGAAATCTGAAGATGACCTCTAGAGGGAAGAAAAGGTTAAATTTTGCGTAGATATATTCCAAGTTTATTTTTTTATATCAGTCTGATGTGTGGAATTAGTGTAGGGCTATTACATAACAATAAATGCAACTTGAAGTTTAAAATAGTCAGTGATGGGCATTCTAGATCATGGGATGTCCACTATCCATTCCCTTTGCCCAAATATTTATTCTCAAATCTCAAATCGGCCAGTTTGTCTGGTAACATCTAATTTCCGTAAATTCTATTAATTGGTATCTATATTACCTTTTCTTTTGATGTGAACTGGTTGGCAAATCCTTGAGAAGTTTTCCAGTTACTAGAATAAGATTAAAACTACTGAACTTGTAAGCTGGCATGGCTAGAGATTTCTAAATGTCTGTGTTTTAGAGATGAGGCAAAATGTCTAATATTAATAAATAATTAAATGTATCATAACTAATCAACTTAAACTTTTTAGTTGAGTAATGATTTAACACAAAACATCTACATTTCAGAACTTCCCATTAGAAATTACATTTCTAAAATCATATTGTAGTAGTTGATAGATATATCTCTACCTTTGCAATTGCAGGTAGGCTAACGGCCAGGTGTGATGTGTATAGCTTTGGGGTTGTGCTGCTGGAGCTCCTCTCAGGACGCCGTGCAGTCGATAAAACTAAAGTTGGCGTGGAGCAGCATCTTGTGGATTGGGCGAAACCTTACTTGGCTGATAAGCGCAAGTTATTTCGGATAATGGACACCAAGTTAGAGGGTCAATACCCTCAAAGAGCTGCTTACATGACTACGATTCTTGCATTACAGTGCATTAGGGAGGCTAAATTCCGACCACAGATGTCCGAAGTCTTATCCGCTCTCGAGCAGCTCCCCCTCGTTAAGCATGCCGATAATCAAAGAGAAAGAGAAAGAGCCCCCAGTCCAGGGCCGAAGTCCCCGTTAAGACACAAGTACCCTTCACCTGTAAATTCCACTGCATCTAAAGGAGGAGGCTCTCCATTACCATCATCCAAATGAAAATGAAGACCCTAGAGAGGGACATGTTCTTTTAACAATGACCCAAGTAAATATTCTTGACTCTCTCGTTTGGCCTGGTAGATTCTAGTCGAAAATTTCGCCCGCCCCTCGGTGTTCGGTCTTCGGTCTTCGGTCTGCATATGAAGAGGAATTGCTGTTGGATGTGGAGTTCTTGTTAACAGCACAGCACAGCAGAGCAGTAGATGAAATGGAGCTGATTTTTGTTTGGGAGGTGGGAAGATGTTGGGTAGAGAGGAGCCACAATAATTTTGCAGAGAATATTACCTTTAGCTGTAAGGGATGGAAATATATATGATGAGTAGCGTCAATATCATTATAAAGTGAGTTGCTTTACCCTGAGGGATTGTATTCAGAATTTGAGATTGAGAACCAAACCTTCAAAAAAATATTTATATTACGGAAGCTTTCTTTTGGTTTTTGGCTGTCCTTTACTCATAGGGACAGATACAAAAGAAAAAGGTAATCACAATTCACAATGATTTTAGGAGATTGTGACATCTGATCT

mRNA sequence

GAAGAACCAAAAAAATCTGAGATGAAAGACCATTCATCGGCTACTTTGAAAGCTCCATCACTTGGGGTGAATGTTCCATCTAGAAGTCTTCAGGCTCATAGATCTGAAGGTGAAATATTGTTGTCCCCCAATCTGAAAGCCTTCACATTCAGTGAGCTTAAGAATGCCACTAAAAACTTCCGCCCTGACAGTCTCATTGGAGAGGGAGGTTTCGGTTATGTGTACAAAGGATGGATTGATGAACATACACTTGAAGCTGTGAGGCCTGGAGCTGGAATGGTTATTGCTGTCAAGAAGCTTAAACCTGAAGGTTTTCAAGGGCACAAGGAATGGCTGTCTGAAGTAAATTATCTCGGTCAGCTTCATCATTCAAATCTTGTGAAACTAATTGGATTCTGCCTTGATGGGGATAGCCGACTTTTGGTCTACGAGTACATGTCCAAAGGCAGCCTTGAAAACCATCTATTTAGAAGGGGTGCCCGACCACTTTCTTGGGCAATAAGGATTAAAGTTGCTATAGGTGCTGCTCGAGGGCTTTCCTTTCTGCATAACTCTGAAGAACCAGTTATCTATCGCGATTTTAAGGCATCAAACATCCTTCTAGATTCTGAATTCAACGCAAAACTTTCAGATTTTGGCTTGGCAAAAGCTGGCCCAACTGGTGATCGGACTCATGTGTCCACTCAAGTCATGGGTACTCAAGGCTATGCTGCTCCAGAATATATTGCCACTGGTAGGCTAACGGCCAGGTGTGATGTGTATAGCTTTGGGGTTGTGCTGCTGGAGCTCCTCTCAGGACGCCGTGCAGTCGATAAAACTAAAGTTGGCGTGGAGCAGCATCTTGTGGATTGGGCGAAACCTTACTTGGCTGATAAGCGCAAGTTATTTCGGATAATGGACACCAAGTTAGAGGGTCAATACCCTCAAAGAGCTGCTTACATGACTACGATTCTTGCATTACAGTGCATTAGGGAGGCTAAATTCCGACCACAGATGTCCGAAGTCTTATCCGCTCTCGAGCAGCTCCCCCTCGTTAAGCATGCCGATAATCAAAGAGAAAGAGAAAGAGCCCCCAGTCCAGGGCCGAAGTCCCCGTTAAGACACAAGTACCCTTCACCTGTAAATTCCACTGCATCTAAAGGAGGAGGCTCTCCATTACCATCATCCAAATGAAAATGAAGACCCTAGAGAGGGACATGTTCTTTTAACAATGACCCAAGTAAATATTCTTGACTCTCTCGTTTGGCCTGGTAGATTCTAGTCGAAAATTTCGCCCGCCCCTCGGTGTTCGGTCTTCGGTCTTCGGTCTGCATATGAAGAGGAATTGCTGTTGGATGTGGAGTTCTTGTTAACAGCACAGCACAGCAGAGCAGTAGATGAAATGGAGCTGATTTTTGTTTGGGAGGTGGGAAGATGTTGGGTAGAGAGGAGCCACAATAATTTTGCAGAGAATATTACCTTTAGCTGTAAGGGATGGAAATATATATGATGAGTAGCGTCAATATCATTATAAAGTGAGTTGCTTTACCCTGAGGGATTGTATTCAGAATTTGAGATTGAGAACCAAACCTTCAAAAAAATATTTATATTACGGAAGCTTTCTTTTGGTTTTTGGCTGTCCTTTACTCATAGGGACAGATACAAAAGAAAAAGGTAATCACAATTCACAATGATTTTAGGAGATTGTGACATCTGATCT

Coding sequence (CDS)

GAAGAACCAAAAAAATCTGAGATGAAAGACCATTCATCGGCTACTTTGAAAGCTCCATCACTTGGGGTGAATGTTCCATCTAGAAGTCTTCAGGCTCATAGATCTGAAGGTGAAATATTGTTGTCCCCCAATCTGAAAGCCTTCACATTCAGTGAGCTTAAGAATGCCACTAAAAACTTCCGCCCTGACAGTCTCATTGGAGAGGGAGGTTTCGGTTATGTGTACAAAGGATGGATTGATGAACATACACTTGAAGCTGTGAGGCCTGGAGCTGGAATGGTTATTGCTGTCAAGAAGCTTAAACCTGAAGGTTTTCAAGGGCACAAGGAATGGCTGTCTGAAGTAAATTATCTCGGTCAGCTTCATCATTCAAATCTTGTGAAACTAATTGGATTCTGCCTTGATGGGGATAGCCGACTTTTGGTCTACGAGTACATGTCCAAAGGCAGCCTTGAAAACCATCTATTTAGAAGGGGTGCCCGACCACTTTCTTGGGCAATAAGGATTAAAGTTGCTATAGGTGCTGCTCGAGGGCTTTCCTTTCTGCATAACTCTGAAGAACCAGTTATCTATCGCGATTTTAAGGCATCAAACATCCTTCTAGATTCTGAATTCAACGCAAAACTTTCAGATTTTGGCTTGGCAAAAGCTGGCCCAACTGGTGATCGGACTCATGTGTCCACTCAAGTCATGGGTACTCAAGGCTATGCTGCTCCAGAATATATTGCCACTGGTAGGCTAACGGCCAGGTGTGATGTGTATAGCTTTGGGGTTGTGCTGCTGGAGCTCCTCTCAGGACGCCGTGCAGTCGATAAAACTAAAGTTGGCGTGGAGCAGCATCTTGTGGATTGGGCGAAACCTTACTTGGCTGATAAGCGCAAGTTATTTCGGATAATGGACACCAAGTTAGAGGGTCAATACCCTCAAAGAGCTGCTTACATGACTACGATTCTTGCATTACAGTGCATTAGGGAGGCTAAATTCCGACCACAGATGTCCGAAGTCTTATCCGCTCTCGAGCAGCTCCCCCTCGTTAAGCATGCCGATAATCAAAGAGAAAGAGAAAGAGCCCCCAGTCCAGGGCCGAAGTCCCCGTTAAGACACAAGTACCCTTCACCTGTAAATTCCACTGCATCTAAAGGAGGAGGCTCTCCATTACCATCATCCAAATGA

Protein sequence

EEPKKSEMKDHSSATLKAPSLGVNVPSRSLQAHRSEGEILLSPNLKAFTFSELKNATKNFRPDSLIGEGGFGYVYKGWIDEHTLEAVRPGAGMVIAVKKLKPEGFQGHKEWLSEVNYLGQLHHSNLVKLIGFCLDGDSRLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGLSFLHNSEEPVIYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPEYIATGRLTARCDVYSFGVVLLELLSGRRAVDKTKVGVEQHLVDWAKPYLADKRKLFRIMDTKLEGQYPQRAAYMTTILALQCIREAKFRPQMSEVLSALEQLPLVKHADNQRERERAPSPGPKSPLRHKYPSPVNSTASKGGGSPLPSSK
Homology
BLAST of MC03g1115 vs. ExPASy Swiss-Prot
Match: O49840 (Probable serine/threonine-protein kinase PBL3 OS=Arabidopsis thaliana OX=3702 GN=PBL3 PE=1 SV=1)

HSP 1 Score: 519.6 bits (1337), Expect = 3.0e-146
Identity = 266/394 (67.51%), Postives = 316/394 (80.20%), Query Frame = 0

Query: 6   SEMKDHSSATLKAPSLGVNVPS--RSLQAHRSEGEILLSPNLKAFTFSELKNATKNFRPD 65
           S +   +S +    SL +N  S   SL   R+EGEIL SPNLKAFTF+ELKNAT+NFRPD
Sbjct: 27  SRVSSKTSRSTVPSSLSINSYSSVESLPTPRTEGEILSSPNLKAFTFNELKNATRNFRPD 86

Query: 66  SLIGEGGFGYVYKGWIDEHTLEAVRPGAGMVIAVKKLKPEGFQGHKEWLSEVNYLGQLHH 125
           SL+GEGGFGYV+KGWID  TL A +PG+G+V+AVKKLK EG+QGHKEWL+EVNYLGQL H
Sbjct: 87  SLLGEGGFGYVFKGWIDGTTLTASKPGSGIVVAVKKLKTEGYQGHKEWLTEVNYLGQLSH 146

Query: 126 SNLVKLIGFCLDGDSRLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGLSFLH 185
            NLVKL+G+C++G++RLLVYE+M KGSLENHLFRRGA+PL+WAIR+KVAIGAA+GL+FLH
Sbjct: 147 PNLVKLVGYCVEGENRLLVYEFMPKGSLENHLFRRGAQPLTWAIRMKVAIGAAKGLTFLH 206

Query: 186 NSEEPVIYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPEYIA 245
           +++  VIYRDFKA+NILLD+EFN+KLSDFGLAKAGPTGD+THVSTQVMGT GYAAPEY+A
Sbjct: 207 DAKSQVIYRDFKAANILLDAEFNSKLSDFGLAKAGPTGDKTHVSTQVMGTHGYAAPEYVA 266

Query: 246 TGRLTARCDVYSFGVVLLELLSGRRAVDKTKVGVEQHLVDWAKPYLADKRKLFRIMDTKL 305
           TGRLTA+ DVYSFGVVLLELLSGRRAVDK+KVG+EQ LVDWA PYL DKRKLFRIMDT+L
Sbjct: 267 TGRLTAKSDVYSFGVVLLELLSGRRAVDKSKVGMEQSLVDWATPYLGDKRKLFRIMDTRL 326

Query: 306 EGQYPQRAAYMTTILALQCIR-EAKFRPQMSEVLSALEQLPLVKHADNQRERE-RAPSPG 365
            GQYPQ+ AY    LALQC+  +AK RP+MSEVL+ L+QL   K       R+ +  SP 
Sbjct: 327 GGQYPQKGAYTAASLALQCLNPDAKLRPKMSEVLAKLDQLESTKPGTGVGNRQAQIDSPR 386

Query: 366 -------PKSPLRHKYPSPVNSTASKGGGSPLPS 389
                   KSP R+ Y  P+       G SPLP+
Sbjct: 387 GSNGSIVQKSPRRYSYDRPLLHITP--GASPLPT 418

BLAST of MC03g1115 vs. ExPASy Swiss-Prot
Match: Q5PP29 (Probable serine/threonine-protein kinase PBL4 OS=Arabidopsis thaliana OX=3702 GN=PBL4 PE=2 SV=1)

HSP 1 Score: 503.1 bits (1294), Expect = 2.9e-141
Identity = 252/343 (73.47%), Postives = 287/343 (83.67%), Query Frame = 0

Query: 4   KKSEMKDHSSATLKAPSLGVNVPSRSLQAHRSEGEILLSPNLKAFTFSELKNATKNFRPD 63
           K+S+    SS T+++ S   +    SLQ  RSEGE+L SP LKAFTF+ELK AT+NFRPD
Sbjct: 27  KRSQSSRLSSLTIQSSSYNDDTSVASLQTPRSEGELLASPTLKAFTFNELKTATRNFRPD 86

Query: 64  SLIGEGGFGYVYKGWIDEHTLEAVRPGAGMVIAVKKLKPEGFQGHKEWLSEVNYLGQLHH 123
           S+IGEGGFGYVYKGWIDE TL   +PG+GMV+AVKKLK EGFQGH++WL+EV+ LG+LHH
Sbjct: 87  SVIGEGGFGYVYKGWIDERTLSPSKPGSGMVVAVKKLKEEGFQGHRQWLAEVDCLGRLHH 146

Query: 124 SNLVKLIGFCLDGDS-RLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGLSFL 183
            NLVKLIG+C  GD  RLLVYEYM KGSLENHLFRRGA P+ W  RIKVAIGAARGL+FL
Sbjct: 147 MNLVKLIGYCSKGDHIRLLVYEYMPKGSLENHLFRRGAEPIPWRTRIKVAIGAARGLAFL 206

Query: 184 HNSEEPVIYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPEYI 243
           H  E  VIYRDFKASNILLDSEFNAKLSDFGLAK GPTGDRTHVSTQVMGTQGYAAPEY+
Sbjct: 207 H--EAQVIYRDFKASNILLDSEFNAKLSDFGLAKVGPTGDRTHVSTQVMGTQGYAAPEYV 266

Query: 244 ATGRLTARCDVYSFGVVLLELLSGRRAVDKTKVGVEQHLVDWAKPYLADKRKLFRIMDTK 303
           ATGR+TA+ DVYSFGVVLLELLSGR  VDKTKVGVE++LVDWA PYL DKRK+FRIMDTK
Sbjct: 267 ATGRITAKSDVYSFGVVLLELLSGRLTVDKTKVGVERNLVDWAIPYLGDKRKVFRIMDTK 326

Query: 304 LEGQYPQRAAYMTTILALQCI-REAKFRPQMSEVLSALEQLPL 345
           L GQYP + A +T   ALQC+ +E K RP+MS+VLS LE+L +
Sbjct: 327 LGGQYPHKGACLTANTALQCLNQEPKLRPKMSDVLSTLEELEM 367

BLAST of MC03g1115 vs. ExPASy Swiss-Prot
Match: O49839 (Probable serine/threonine-protein kinase PBL2 OS=Arabidopsis thaliana OX=3702 GN=PBL2 PE=1 SV=1)

HSP 1 Score: 499.2 bits (1284), Expect = 4.2e-140
Identity = 249/361 (68.98%), Postives = 295/361 (81.72%), Query Frame = 0

Query: 34  RSEGEILLSPNLKAFTFSELKNATKNFRPDSLIGEGGFGYVYKGWIDEHTLEAVRPGAGM 93
           R+EGEIL SPNLKAFTF+ELKNATKNFR D+L+GEGGFG V+KGWID+ +L A RPG+G+
Sbjct: 60  RTEGEILSSPNLKAFTFNELKNATKNFRQDNLLGEGGFGCVFKGWIDQTSLTASRPGSGI 119

Query: 94  VIAVKKLKPEGFQGHKEWLSEVNYLGQLHHSNLVKLIGFCLDGDSRLLVYEYMSKGSLEN 153
           V+AVK+LKPEGFQGHKEWL+EVNYLGQL H NLV L+G+C +G++RLLVYE+M KGSLEN
Sbjct: 120 VVAVKQLKPEGFQGHKEWLTEVNYLGQLSHPNLVLLVGYCAEGENRLLVYEFMPKGSLEN 179

Query: 154 HLFRRGARPLSWAIRIKVAIGAARGLSFLHNSEEPVIYRDFKASNILLDSEFNAKLSDFG 213
           HLFRRGA+PL+WAIR+KVA+GAA+GL+FLH ++  VIYRDFKA+NILLD++FNAKLSDFG
Sbjct: 180 HLFRRGAQPLTWAIRMKVAVGAAKGLTFLHEAKSQVIYRDFKAANILLDADFNAKLSDFG 239

Query: 214 LAKAGPTGDRTHVSTQVMGTQGYAAPEYIATGRLTARCDVYSFGVVLLELLSGRRAVDKT 273
           LAKAGPTGD THVST+V+GT GYAAPEY+ATGRLTA+ DVYSFGVVLLEL+SGRRA+D +
Sbjct: 240 LAKAGPTGDNTHVSTKVIGTHGYAAPEYVATGRLTAKSDVYSFGVVLLELISGRRAMDNS 299

Query: 274 KVGVEQHLVDWAKPYLADKRKLFRIMDTKLEGQYPQRAAYMTTILALQCIR-EAKFRPQM 333
             G E  LVDWA PYL DKRKLFRIMDTKL GQYPQ+ A+    LALQC+  +AK RP+M
Sbjct: 300 NGGNEYSLVDWATPYLGDKRKLFRIMDTKLGGQYPQKGAFTAANLALQCLNPDAKLRPKM 359

Query: 334 SEVLSALEQL-----PLVKHADNQRERERAPSPGPKSPLRHKYPSPVNSTASKGGGSPLP 389
           SEVL  LEQL     P  KH   +  R    S   KSP+R+ +  P+       G SPLP
Sbjct: 360 SEVLVTLEQLESVAKPGTKHTQMESPRFHHSSVMQKSPVRYSHDRPLLHMTP--GASPLP 418

BLAST of MC03g1115 vs. ExPASy Swiss-Prot
Match: Q5XF79 (Probable serine/threonine-protein kinase PBL18 OS=Arabidopsis thaliana OX=3702 GN=PBL18 PE=2 SV=1)

HSP 1 Score: 476.9 bits (1226), Expect = 2.2e-133
Identity = 231/310 (74.52%), Postives = 267/310 (86.13%), Query Frame = 0

Query: 34  RSEGEILLSPNLKAFTFSELKNATKNFRPDSLIGEGGFGYVYKGWIDEHTLEAVRPGAGM 93
           RSEGE+L SP LKAFTF+ELK AT+NF+P+S+IGEGGFG VYKGWI E +L   +PG+GM
Sbjct: 58  RSEGELLPSPTLKAFTFNELKTATRNFKPNSMIGEGGFGCVYKGWIGERSLSPSKPGSGM 117

Query: 94  VIAVKKLKPEGFQGHKEWLSEVNYLGQLHHSNLVKLIGFCLDGDSRLLVYEYMSKGSLEN 153
           V+AVKKLK EGFQGHKEWL+EV+YLG+LHH NLVKLIG+CL+G+ RLLVYEYM KGSLEN
Sbjct: 118 VVAVKKLKSEGFQGHKEWLTEVHYLGRLHHMNLVKLIGYCLEGEKRLLVYEYMPKGSLEN 177

Query: 154 HLFRRGARPLSWAIRIKVAIGAARGLSFLHNSEEPVIYRDFKASNILLDSEFNAKLSDFG 213
           HLFRRGA P+ W  R+KVA  AARGLSFLH  E  VIYRDFKASNILLD +FNAKLSDFG
Sbjct: 178 HLFRRGAEPIPWKTRMKVAFSAARGLSFLH--EAKVIYRDFKASNILLDVDFNAKLSDFG 237

Query: 214 LAKAGPTGDRTHVSTQVMGTQGYAAPEYIATGRLTARCDVYSFGVVLLELLSGRRAVDKT 273
           LAKAGPTGDRTHV+TQV+GTQGYAAPEYIATGRLT++ DVYSFGVVLLELLSGR  +DK+
Sbjct: 238 LAKAGPTGDRTHVTTQVIGTQGYAAPEYIATGRLTSKSDVYSFGVVLLELLSGRPTLDKS 297

Query: 274 KVGVEQHLVDWAKPYLADKRKLFRIMDTKLEGQYPQRAAYMTTILALQCIR-EAKFRPQM 333
           KVGVE++LVDWA PYL D+RK+FRIMDTKL GQYP + A     +AL+C+  E K RP M
Sbjct: 298 KVGVERNLVDWAIPYLVDRRKVFRIMDTKLGGQYPHKGACAAANIALRCLNTEPKLRPDM 357

Query: 334 SEVLSALEQL 343
           ++VLS L+QL
Sbjct: 358 ADVLSTLQQL 365

BLAST of MC03g1115 vs. ExPASy Swiss-Prot
Match: P46573 (Probable serine/threonine-protein kinase PBL10 OS=Arabidopsis thaliana OX=3702 GN=PBL10 PE=1 SV=2)

HSP 1 Score: 451.8 bits (1161), Expect = 7.7e-126
Identity = 220/333 (66.07%), Postives = 272/333 (81.68%), Query Frame = 0

Query: 20  SLGVNVPSRSLQAH-RSEGEILLSPNLKAFTFSELKNATKNFRPDSLIGEGGFGYVYKGW 79
           SLG    S S++ + R+EGEIL SPNLK+FTF+ELK AT+NFRPDS++GEGGFG V+KGW
Sbjct: 28  SLGSKSSSVSIRTNPRTEGEILQSPNLKSFTFAELKAATRNFRPDSVLGEGGFGSVFKGW 87

Query: 80  IDEHTLEAVRPGAGMVIAVKKLKPEGFQGHKEWLSEVNYLGQLHHSNLVKLIGFCLDGDS 139
           IDE TL A +PG G+VIAVKKL  +G+QGH+EWL+EVNYLGQ  H NLVKLIG+CL+ + 
Sbjct: 88  IDEQTLTASKPGTGVVIAVKKLNQDGWQGHQEWLAEVNYLGQFSHPNLVKLIGYCLEDEH 147

Query: 140 RLLVYEYMSKGSLENHLFRRGA--RPLSWAIRIKVAIGAARGLSFLHNSEEPVIYRDFKA 199
           RLLVYE+M +GSLENHLFRRG+  +PLSW +R+KVA+GAA+GL+FLHN+E  VIYRDFK 
Sbjct: 148 RLLVYEFMPRGSLENHLFRRGSYFQPLSWTLRLKVALGAAKGLAFLHNAETSVIYRDFKT 207

Query: 200 SNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPEYIATGRLTARCDVYSF 259
           SNILLDSE+NAKLSDFGLAK GPTGD++HVST++MGT GYAAPEY+ATG LT + DVYS+
Sbjct: 208 SNILLDSEYNAKLSDFGLAKDGPTGDKSHVSTRIMGTYGYAAPEYLATGHLTTKSDVYSY 267

Query: 260 GVVLLELLSGRRAVDKTKVGVEQHLVDWAKPYLADKRKLFRIMDTKLEGQYPQRAAYMTT 319
           GVVLLE+LSGRRAVDK +   EQ LV+WA+P LA+KRKLFR++D +L+ QY    A    
Sbjct: 268 GVVLLEVLSGRRAVDKNRPPGEQKLVEWARPLLANKRKLFRVIDNRLQDQYSMEEACKVA 327

Query: 320 ILALQCIR-EAKFRPQMSEVLSALEQLPLVKHA 349
            LAL+C+  E K RP M+EV+S LE +  +  A
Sbjct: 328 TLALRCLTFEIKLRPNMNEVVSHLEHIQTLNEA 360

BLAST of MC03g1115 vs. NCBI nr
Match: XP_022137441.1 (probable serine/threonine-protein kinase PBL3 [Momordica charantia])

HSP 1 Score: 775 bits (2002), Expect = 2.25e-282
Identity = 389/389 (100.00%), Postives = 389/389 (100.00%), Query Frame = 0

Query: 2   EPKKSEMKDHSSATLKAPSLGVNVPSRSLQAHRSEGEILLSPNLKAFTFSELKNATKNFR 61
           EPKKSEMKDHSSATLKAPSLGVNVPSRSLQAHRSEGEILLSPNLKAFTFSELKNATKNFR
Sbjct: 22  EPKKSEMKDHSSATLKAPSLGVNVPSRSLQAHRSEGEILLSPNLKAFTFSELKNATKNFR 81

Query: 62  PDSLIGEGGFGYVYKGWIDEHTLEAVRPGAGMVIAVKKLKPEGFQGHKEWLSEVNYLGQL 121
           PDSLIGEGGFGYVYKGWIDEHTLEAVRPGAGMVIAVKKLKPEGFQGHKEWLSEVNYLGQL
Sbjct: 82  PDSLIGEGGFGYVYKGWIDEHTLEAVRPGAGMVIAVKKLKPEGFQGHKEWLSEVNYLGQL 141

Query: 122 HHSNLVKLIGFCLDGDSRLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGLSF 181
           HHSNLVKLIGFCLDGDSRLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGLSF
Sbjct: 142 HHSNLVKLIGFCLDGDSRLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGLSF 201

Query: 182 LHNSEEPVIYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPEY 241
           LHNSEEPVIYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPEY
Sbjct: 202 LHNSEEPVIYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPEY 261

Query: 242 IATGRLTARCDVYSFGVVLLELLSGRRAVDKTKVGVEQHLVDWAKPYLADKRKLFRIMDT 301
           IATGRLTARCDVYSFGVVLLELLSGRRAVDKTKVGVEQHLVDWAKPYLADKRKLFRIMDT
Sbjct: 262 IATGRLTARCDVYSFGVVLLELLSGRRAVDKTKVGVEQHLVDWAKPYLADKRKLFRIMDT 321

Query: 302 KLEGQYPQRAAYMTTILALQCIREAKFRPQMSEVLSALEQLPLVKHADNQRERERAPSPG 361
           KLEGQYPQRAAYMTTILALQCIREAKFRPQMSEVLSALEQLPLVKHADNQRERERAPSPG
Sbjct: 322 KLEGQYPQRAAYMTTILALQCIREAKFRPQMSEVLSALEQLPLVKHADNQRERERAPSPG 381

Query: 362 PKSPLRHKYPSPVNSTASKGGGSPLPSSK 390
           PKSPLRHKYPSPVNSTASKGGGSPLPSSK
Sbjct: 382 PKSPLRHKYPSPVNSTASKGGGSPLPSSK 410

BLAST of MC03g1115 vs. NCBI nr
Match: XP_008437450.1 (PREDICTED: protein kinase 2B, chloroplastic-like [Cucumis melo])

HSP 1 Score: 696 bits (1795), Expect = 6.77e-251
Identity = 349/388 (89.95%), Postives = 366/388 (94.33%), Query Frame = 0

Query: 1   EEPKKSEMKDHSSATLKAPSLGVNVPSRSLQAHRSEGEILLSPNLKAFTFSELKNATKNF 60
           E+PK  + K HSSA+L+APSLG N PSR LQ HR+EGEILLSPNLKAFT+SELKNATKNF
Sbjct: 23  EDPKAHDRKGHSSASLRAPSLGGNTPSRGLQVHRTEGEILLSPNLKAFTYSELKNATKNF 82

Query: 61  RPDSLIGEGGFGYVYKGWIDEHTLEAVRPGAGMVIAVKKLKPEGFQGHKEWLSEVNYLGQ 120
           RPDSLIGEGGFG+VYKGWIDEHTLEAVRPGAGMV+AVKKLKPEGFQGHKEWLSEVNYLGQ
Sbjct: 83  RPDSLIGEGGFGHVYKGWIDEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWLSEVNYLGQ 142

Query: 121 LHHSNLVKLIGFCLDGDSRLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGLS 180
           LHH NLVKLIGFCLDGDSRLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGL+
Sbjct: 143 LHHPNLVKLIGFCLDGDSRLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGLT 202

Query: 181 FLHNSEEPVIYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPE 240
           FLH+SEEPVIYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPE
Sbjct: 203 FLHDSEEPVIYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPE 262

Query: 241 YIATGRLTARCDVYSFGVVLLELLSGRRAVDKTKVGVEQHLVDWAKPYLADKRKLFRIMD 300
           YIATGRLTA+CDVYSFGVVLLELLSGRRAVDKTKVGVEQ+LV+WA+PYLADKRKLFRIMD
Sbjct: 263 YIATGRLTAKCDVYSFGVVLLELLSGRRAVDKTKVGVEQNLVEWARPYLADKRKLFRIMD 322

Query: 301 TKLEGQYPQRAAYMTTILALQCIREAKFRPQMSEVLSALEQLPLVKHADNQRERERAPSP 360
            KLEGQYPQ+AAYMTT+LALQCIREAKFRPQMSEVLSALEQLPLVKH D QR    A SP
Sbjct: 323 IKLEGQYPQKAAYMTTVLALQCIREAKFRPQMSEVLSALEQLPLVKHVDTQR----ANSP 382

Query: 361 GPKSPLRHKYPSPVNSTASKGGGSPLPS 388
           GPKSPLRHKYPSP+NST  K   SPLPS
Sbjct: 383 GPKSPLRHKYPSPLNSTTPKAS-SPLPS 405

BLAST of MC03g1115 vs. NCBI nr
Match: XP_038895448.1 (probable serine/threonine-protein kinase PBL3 isoform X1 [Benincasa hispida])

HSP 1 Score: 697 bits (1798), Expect = 1.69e-250
Identity = 351/388 (90.46%), Postives = 365/388 (94.07%), Query Frame = 0

Query: 1   EEPKKSEMKDHSSATLKAPSLGVNVPSRSLQAHRSEGEILLSPNLKAFTFSELKNATKNF 60
           EEPK  + K HSSA+L+APSLG N PSR LQ HRSEGEILLSPNLKAFT+SELKNATKNF
Sbjct: 76  EEPKNHDRKGHSSASLRAPSLGGNTPSRGLQVHRSEGEILLSPNLKAFTYSELKNATKNF 135

Query: 61  RPDSLIGEGGFGYVYKGWIDEHTLEAVRPGAGMVIAVKKLKPEGFQGHKEWLSEVNYLGQ 120
           RPDSLIGEGGFG+VYKGWIDEHTLEAVRPGAGMV+AVKKLKPEGFQGHKEWLSEVNYLGQ
Sbjct: 136 RPDSLIGEGGFGHVYKGWIDEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWLSEVNYLGQ 195

Query: 121 LHHSNLVKLIGFCLDGDSRLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGLS 180
           LHH NLVKLIGFCLDGDSRLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGL+
Sbjct: 196 LHHPNLVKLIGFCLDGDSRLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGLT 255

Query: 181 FLHNSEEPVIYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPE 240
           FLH+SEEPVIYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPE
Sbjct: 256 FLHDSEEPVIYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPE 315

Query: 241 YIATGRLTARCDVYSFGVVLLELLSGRRAVDKTKVGVEQHLVDWAKPYLADKRKLFRIMD 300
           YIATGRLTA+CDVYSFGVVLLELLSGRRAVDKTKVGVEQ+LV+WA+PYLADKRKLFRIMD
Sbjct: 316 YIATGRLTAKCDVYSFGVVLLELLSGRRAVDKTKVGVEQNLVEWARPYLADKRKLFRIMD 375

Query: 301 TKLEGQYPQRAAYMTTILALQCIREAKFRPQMSEVLSALEQLPLVKHADNQRERERAPSP 360
            KLEGQYPQ+AAYMTT+LALQCIREAKFRPQMSEVL ALEQLPLVKH  NQR    A SP
Sbjct: 376 IKLEGQYPQKAAYMTTVLALQCIREAKFRPQMSEVLYALEQLPLVKHVHNQR----ANSP 435

Query: 361 GPKSPLRHKYPSPVNSTASKGGGSPLPS 388
           GPKSPLRHKYPSPVNST  K   SPLPS
Sbjct: 436 GPKSPLRHKYPSPVNSTTPKAS-SPLPS 458

BLAST of MC03g1115 vs. NCBI nr
Match: XP_011651142.1 (probable serine/threonine-protein kinase PBL3 isoform X1 [Cucumis sativus] >KAE8652573.1 hypothetical protein Csa_013876 [Cucumis sativus])

HSP 1 Score: 689 bits (1778), Expect = 2.63e-248
Identity = 346/388 (89.18%), Postives = 364/388 (93.81%), Query Frame = 0

Query: 1   EEPKKSEMKDHSSATLKAPSLGVNVPSRSLQAHRSEGEILLSPNLKAFTFSELKNATKNF 60
           E+PK  + K HSSA+L++PSLG N PSR LQ HR+EGEILLSPNLKAFT+SELKNATKNF
Sbjct: 23  EDPKTHDRKGHSSASLRSPSLGGNTPSRGLQVHRTEGEILLSPNLKAFTYSELKNATKNF 82

Query: 61  RPDSLIGEGGFGYVYKGWIDEHTLEAVRPGAGMVIAVKKLKPEGFQGHKEWLSEVNYLGQ 120
           RPDSLIGEGGFG+VYKGWIDEHTLEAVRPGAGMV+AVKKLKPEGFQGHKEWLSEVNYLGQ
Sbjct: 83  RPDSLIGEGGFGHVYKGWIDEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWLSEVNYLGQ 142

Query: 121 LHHSNLVKLIGFCLDGDSRLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGLS 180
           LHH NLVKLIGFCLDGDSRLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGL+
Sbjct: 143 LHHPNLVKLIGFCLDGDSRLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGLT 202

Query: 181 FLHNSEEPVIYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPE 240
           FLH+SEEPVIYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPE
Sbjct: 203 FLHDSEEPVIYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPE 262

Query: 241 YIATGRLTARCDVYSFGVVLLELLSGRRAVDKTKVGVEQHLVDWAKPYLADKRKLFRIMD 300
           YIATGRLTA+CDVYSFGVVLLELLSGRRAVDKTKVGVEQ+LV+WA+PYLADKRKLFRIMD
Sbjct: 263 YIATGRLTAKCDVYSFGVVLLELLSGRRAVDKTKVGVEQNLVEWARPYLADKRKLFRIMD 322

Query: 301 TKLEGQYPQRAAYMTTILALQCIREAKFRPQMSEVLSALEQLPLVKHADNQRERERAPSP 360
            KLEGQYPQ+AAYMTT+LALQCIREAKFRPQMSEVL ALEQLPLVKH D QR    A SP
Sbjct: 323 IKLEGQYPQKAAYMTTVLALQCIREAKFRPQMSEVLYALEQLPLVKHVDTQR----ANSP 382

Query: 361 GPKSPLRHKYPSPVNSTASKGGGSPLPS 388
           GPKSPLRHKYPSP+NS   K   SPLPS
Sbjct: 383 GPKSPLRHKYPSPLNSMTPKPS-SPLPS 405

BLAST of MC03g1115 vs. NCBI nr
Match: XP_031745128.1 (probable serine/threonine-protein kinase PBL3 isoform X2 [Cucumis sativus])

HSP 1 Score: 687 bits (1774), Expect = 1.03e-247
Identity = 345/387 (89.15%), Postives = 363/387 (93.80%), Query Frame = 0

Query: 2   EPKKSEMKDHSSATLKAPSLGVNVPSRSLQAHRSEGEILLSPNLKAFTFSELKNATKNFR 61
           +PK  + K HSSA+L++PSLG N PSR LQ HR+EGEILLSPNLKAFT+SELKNATKNFR
Sbjct: 23  DPKTHDRKGHSSASLRSPSLGGNTPSRGLQVHRTEGEILLSPNLKAFTYSELKNATKNFR 82

Query: 62  PDSLIGEGGFGYVYKGWIDEHTLEAVRPGAGMVIAVKKLKPEGFQGHKEWLSEVNYLGQL 121
           PDSLIGEGGFG+VYKGWIDEHTLEAVRPGAGMV+AVKKLKPEGFQGHKEWLSEVNYLGQL
Sbjct: 83  PDSLIGEGGFGHVYKGWIDEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWLSEVNYLGQL 142

Query: 122 HHSNLVKLIGFCLDGDSRLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGLSF 181
           HH NLVKLIGFCLDGDSRLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGL+F
Sbjct: 143 HHPNLVKLIGFCLDGDSRLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGLTF 202

Query: 182 LHNSEEPVIYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPEY 241
           LH+SEEPVIYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPEY
Sbjct: 203 LHDSEEPVIYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPEY 262

Query: 242 IATGRLTARCDVYSFGVVLLELLSGRRAVDKTKVGVEQHLVDWAKPYLADKRKLFRIMDT 301
           IATGRLTA+CDVYSFGVVLLELLSGRRAVDKTKVGVEQ+LV+WA+PYLADKRKLFRIMD 
Sbjct: 263 IATGRLTAKCDVYSFGVVLLELLSGRRAVDKTKVGVEQNLVEWARPYLADKRKLFRIMDI 322

Query: 302 KLEGQYPQRAAYMTTILALQCIREAKFRPQMSEVLSALEQLPLVKHADNQRERERAPSPG 361
           KLEGQYPQ+AAYMTT+LALQCIREAKFRPQMSEVL ALEQLPLVKH D QR    A SPG
Sbjct: 323 KLEGQYPQKAAYMTTVLALQCIREAKFRPQMSEVLYALEQLPLVKHVDTQR----ANSPG 382

Query: 362 PKSPLRHKYPSPVNSTASKGGGSPLPS 388
           PKSPLRHKYPSP+NS   K   SPLPS
Sbjct: 383 PKSPLRHKYPSPLNSMTPKPS-SPLPS 404

BLAST of MC03g1115 vs. ExPASy TrEMBL
Match: A0A6J1C6M0 (probable serine/threonine-protein kinase PBL3 OS=Momordica charantia OX=3673 GN=LOC111008885 PE=3 SV=1)

HSP 1 Score: 775 bits (2002), Expect = 1.09e-282
Identity = 389/389 (100.00%), Postives = 389/389 (100.00%), Query Frame = 0

Query: 2   EPKKSEMKDHSSATLKAPSLGVNVPSRSLQAHRSEGEILLSPNLKAFTFSELKNATKNFR 61
           EPKKSEMKDHSSATLKAPSLGVNVPSRSLQAHRSEGEILLSPNLKAFTFSELKNATKNFR
Sbjct: 22  EPKKSEMKDHSSATLKAPSLGVNVPSRSLQAHRSEGEILLSPNLKAFTFSELKNATKNFR 81

Query: 62  PDSLIGEGGFGYVYKGWIDEHTLEAVRPGAGMVIAVKKLKPEGFQGHKEWLSEVNYLGQL 121
           PDSLIGEGGFGYVYKGWIDEHTLEAVRPGAGMVIAVKKLKPEGFQGHKEWLSEVNYLGQL
Sbjct: 82  PDSLIGEGGFGYVYKGWIDEHTLEAVRPGAGMVIAVKKLKPEGFQGHKEWLSEVNYLGQL 141

Query: 122 HHSNLVKLIGFCLDGDSRLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGLSF 181
           HHSNLVKLIGFCLDGDSRLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGLSF
Sbjct: 142 HHSNLVKLIGFCLDGDSRLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGLSF 201

Query: 182 LHNSEEPVIYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPEY 241
           LHNSEEPVIYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPEY
Sbjct: 202 LHNSEEPVIYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPEY 261

Query: 242 IATGRLTARCDVYSFGVVLLELLSGRRAVDKTKVGVEQHLVDWAKPYLADKRKLFRIMDT 301
           IATGRLTARCDVYSFGVVLLELLSGRRAVDKTKVGVEQHLVDWAKPYLADKRKLFRIMDT
Sbjct: 262 IATGRLTARCDVYSFGVVLLELLSGRRAVDKTKVGVEQHLVDWAKPYLADKRKLFRIMDT 321

Query: 302 KLEGQYPQRAAYMTTILALQCIREAKFRPQMSEVLSALEQLPLVKHADNQRERERAPSPG 361
           KLEGQYPQRAAYMTTILALQCIREAKFRPQMSEVLSALEQLPLVKHADNQRERERAPSPG
Sbjct: 322 KLEGQYPQRAAYMTTILALQCIREAKFRPQMSEVLSALEQLPLVKHADNQRERERAPSPG 381

Query: 362 PKSPLRHKYPSPVNSTASKGGGSPLPSSK 390
           PKSPLRHKYPSPVNSTASKGGGSPLPSSK
Sbjct: 382 PKSPLRHKYPSPVNSTASKGGGSPLPSSK 410

BLAST of MC03g1115 vs. ExPASy TrEMBL
Match: A0A1S3AU61 (protein kinase 2B, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103482862 PE=3 SV=1)

HSP 1 Score: 696 bits (1795), Expect = 3.28e-251
Identity = 349/388 (89.95%), Postives = 366/388 (94.33%), Query Frame = 0

Query: 1   EEPKKSEMKDHSSATLKAPSLGVNVPSRSLQAHRSEGEILLSPNLKAFTFSELKNATKNF 60
           E+PK  + K HSSA+L+APSLG N PSR LQ HR+EGEILLSPNLKAFT+SELKNATKNF
Sbjct: 23  EDPKAHDRKGHSSASLRAPSLGGNTPSRGLQVHRTEGEILLSPNLKAFTYSELKNATKNF 82

Query: 61  RPDSLIGEGGFGYVYKGWIDEHTLEAVRPGAGMVIAVKKLKPEGFQGHKEWLSEVNYLGQ 120
           RPDSLIGEGGFG+VYKGWIDEHTLEAVRPGAGMV+AVKKLKPEGFQGHKEWLSEVNYLGQ
Sbjct: 83  RPDSLIGEGGFGHVYKGWIDEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWLSEVNYLGQ 142

Query: 121 LHHSNLVKLIGFCLDGDSRLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGLS 180
           LHH NLVKLIGFCLDGDSRLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGL+
Sbjct: 143 LHHPNLVKLIGFCLDGDSRLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGLT 202

Query: 181 FLHNSEEPVIYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPE 240
           FLH+SEEPVIYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPE
Sbjct: 203 FLHDSEEPVIYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPE 262

Query: 241 YIATGRLTARCDVYSFGVVLLELLSGRRAVDKTKVGVEQHLVDWAKPYLADKRKLFRIMD 300
           YIATGRLTA+CDVYSFGVVLLELLSGRRAVDKTKVGVEQ+LV+WA+PYLADKRKLFRIMD
Sbjct: 263 YIATGRLTAKCDVYSFGVVLLELLSGRRAVDKTKVGVEQNLVEWARPYLADKRKLFRIMD 322

Query: 301 TKLEGQYPQRAAYMTTILALQCIREAKFRPQMSEVLSALEQLPLVKHADNQRERERAPSP 360
            KLEGQYPQ+AAYMTT+LALQCIREAKFRPQMSEVLSALEQLPLVKH D QR    A SP
Sbjct: 323 IKLEGQYPQKAAYMTTVLALQCIREAKFRPQMSEVLSALEQLPLVKHVDTQR----ANSP 382

Query: 361 GPKSPLRHKYPSPVNSTASKGGGSPLPS 388
           GPKSPLRHKYPSP+NST  K   SPLPS
Sbjct: 383 GPKSPLRHKYPSPLNSTTPKAS-SPLPS 405

BLAST of MC03g1115 vs. ExPASy TrEMBL
Match: A0A6J1E7M0 (probable serine/threonine-protein kinase PBL3 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111431470 PE=3 SV=1)

HSP 1 Score: 677 bits (1746), Expect = 6.82e-244
Identity = 340/378 (89.95%), Postives = 357/378 (94.44%), Query Frame = 0

Query: 1   EEPKKSEMKDHSSATLKAPSLGVNVPSRSLQAHRSEGEILLSPNLKAFTFSELKNATKNF 60
           EEPKK   K HSSA+L+APSLG + PSR LQAHRSEGEILLSPNLKAFT+SEL+NATKNF
Sbjct: 23  EEPKKHNRKGHSSASLRAPSLGGSTPSRGLQAHRSEGEILLSPNLKAFTYSELRNATKNF 82

Query: 61  RPDSLIGEGGFGYVYKGWIDEHTLEAVRPGAGMVIAVKKLKPEGFQGHKEWLSEVNYLGQ 120
           RPDSLIGEGGFG+VYKGWIDEHTLEAVRPGAGMV+AVKKLKPEGFQGHKEWLSEVNYLGQ
Sbjct: 83  RPDSLIGEGGFGHVYKGWIDEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWLSEVNYLGQ 142

Query: 121 LHHSNLVKLIGFCLDGDSRLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGLS 180
           LHH NLVKLIGFCLDGDSRLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGLS
Sbjct: 143 LHHPNLVKLIGFCLDGDSRLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGLS 202

Query: 181 FLHNSEEPVIYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPE 240
           FLH+SEEPVIYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPE
Sbjct: 203 FLHDSEEPVIYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPE 262

Query: 241 YIATGRLTARCDVYSFGVVLLELLSGRRAVDKTKVGVEQHLVDWAKPYLADKRKLFRIMD 300
           YIATGRLTA+CDVYSFGVVLLELLSGRRAVDKTKVG+EQ+LV+WAKPYLADKRKLFRIMD
Sbjct: 263 YIATGRLTAKCDVYSFGVVLLELLSGRRAVDKTKVGIEQNLVEWAKPYLADKRKLFRIMD 322

Query: 301 TKLEGQYPQRAAYMTTILALQCIREAKFRPQMSEVLSALEQLPLVKHADNQRERERAPSP 360
           TKLEGQYPQ+AAYMTT+LALQCIREAK RPQMSEVLS+LE+LPLVKH DNQR      SP
Sbjct: 323 TKLEGQYPQKAAYMTTVLALQCIREAKLRPQMSEVLSSLEELPLVKHVDNQRLN----SP 382

Query: 361 GPKSPLRH--KYPSPVNS 376
           GPKSPLRH  K  SP+ S
Sbjct: 383 GPKSPLRHTPKASSPLPS 396

BLAST of MC03g1115 vs. ExPASy TrEMBL
Match: A0A6J1KLE7 (probable serine/threonine-protein kinase PBL3 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111495635 PE=3 SV=1)

HSP 1 Score: 676 bits (1743), Expect = 1.95e-243
Identity = 339/378 (89.68%), Postives = 357/378 (94.44%), Query Frame = 0

Query: 1   EEPKKSEMKDHSSATLKAPSLGVNVPSRSLQAHRSEGEILLSPNLKAFTFSELKNATKNF 60
           EEPKK   K HSSA+L+APSLG + PSR LQAHRSEGEILLSPNLKAFT+SEL+NATKNF
Sbjct: 23  EEPKKHNRKGHSSASLRAPSLGGSTPSRGLQAHRSEGEILLSPNLKAFTYSELRNATKNF 82

Query: 61  RPDSLIGEGGFGYVYKGWIDEHTLEAVRPGAGMVIAVKKLKPEGFQGHKEWLSEVNYLGQ 120
           RPDSLIGEGGFG+VYKGWIDEHTLEAVRPGAGMV+AVKKLKPEGFQGHKEWLSEVNYLGQ
Sbjct: 83  RPDSLIGEGGFGHVYKGWIDEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWLSEVNYLGQ 142

Query: 121 LHHSNLVKLIGFCLDGDSRLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGLS 180
           LHH NLVKLIGFCLDGDSRLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGLS
Sbjct: 143 LHHPNLVKLIGFCLDGDSRLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGLS 202

Query: 181 FLHNSEEPVIYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPE 240
           FLH+SEEPVIYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPE
Sbjct: 203 FLHDSEEPVIYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPE 262

Query: 241 YIATGRLTARCDVYSFGVVLLELLSGRRAVDKTKVGVEQHLVDWAKPYLADKRKLFRIMD 300
           YIATGRLTA+CDVYSFGVVLLELLSGRRAVDKTKVG+EQ+LV+WA+PYLADKRKLFRIMD
Sbjct: 263 YIATGRLTAKCDVYSFGVVLLELLSGRRAVDKTKVGIEQNLVEWARPYLADKRKLFRIMD 322

Query: 301 TKLEGQYPQRAAYMTTILALQCIREAKFRPQMSEVLSALEQLPLVKHADNQRERERAPSP 360
           TKLEGQYPQ+AAYMTT+LALQCIREAK RPQMSEVLS+LE+LPLVKH DNQR      SP
Sbjct: 323 TKLEGQYPQKAAYMTTVLALQCIREAKLRPQMSEVLSSLEELPLVKHVDNQRLN----SP 382

Query: 361 GPKSPLRH--KYPSPVNS 376
           GPKSPLRH  K  SP+ S
Sbjct: 383 GPKSPLRHTPKASSPLPS 396

BLAST of MC03g1115 vs. ExPASy TrEMBL
Match: A0A6J1GSG8 (probable serine/threonine-protein kinase PBL3 OS=Cucurbita moschata OX=3662 GN=LOC111457081 PE=3 SV=1)

HSP 1 Score: 640 bits (1650), Expect = 3.83e-229
Identity = 322/388 (82.99%), Postives = 348/388 (89.69%), Query Frame = 0

Query: 1   EEPKKSEMKDHSSATLKAPSLGVNVPSRSLQAHRSEGEILLSPNLKAFTFSELKNATKNF 60
           E P   + K HS+A L+AP+LG N PSR LQ  R+EGEILLSPNLKAFT+SELKNATK+F
Sbjct: 22  ETPNNQDRKGHSTAALRAPALGGNTPSRGLQPKRTEGEILLSPNLKAFTYSELKNATKSF 81

Query: 61  RPDSLIGEGGFGYVYKGWIDEHTLEAVRPGAGMVIAVKKLKPEGFQGHKEWLSEVNYLGQ 120
           RPD+LIGEGGFG+VYKGW+DE TLE+V+PG GMV+AVKKLK EGFQGHKEWLSEV +L Q
Sbjct: 82  RPDTLIGEGGFGHVYKGWLDEQTLESVKPGTGMVVAVKKLKSEGFQGHKEWLSEVTHLSQ 141

Query: 121 LHHSNLVKLIGFCLDGDSRLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGLS 180
           LHH +LVKLIGFCLDG+SRLLVYEYMSKGSLENHLFR GARPLSWA RIKVAIGAARGLS
Sbjct: 142 LHHPHLVKLIGFCLDGESRLLVYEYMSKGSLENHLFRMGARPLSWARRIKVAIGAARGLS 201

Query: 181 FLHNSEEPVIYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPE 240
           FLHNS EPVIYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDR+HVST+V+GTQGYAAPE
Sbjct: 202 FLHNSAEPVIYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRSHVSTRVIGTQGYAAPE 261

Query: 241 YIATGRLTARCDVYSFGVVLLELLSGRRAVDKTKVGVEQHLVDWAKPYLADKRKLFRIMD 300
           YIATG LTA+CDVYSFGVVLLELLSGRR VDKTKVGVEQHLVDWA+PYLADKRKLFRIMD
Sbjct: 262 YIATGMLTAKCDVYSFGVVLLELLSGRRVVDKTKVGVEQHLVDWARPYLADKRKLFRIMD 321

Query: 301 TKLEGQYPQRAAYMTTILALQCIREAKFRPQMSEVLSALEQLPLVKHADNQRERERAPSP 360
           T+LEG+YPQ+AAYMTT+LALQCIREAKFRP MSEVL ALEQL LVK  DNQR    A SP
Sbjct: 322 TRLEGKYPQKAAYMTTVLALQCIREAKFRPLMSEVLYALEQLLLVKPTDNQR----ASSP 381

Query: 361 GPKSPLRHKYPSPVNSTASKGGGSPLPS 388
           GPKSP+ H  PSPVNST  KG  SPLPS
Sbjct: 382 GPKSPITHNLPSPVNSTTQKGS-SPLPS 404

BLAST of MC03g1115 vs. TAIR 10
Match: AT2G02800.1 (protein kinase 2B )

HSP 1 Score: 519.6 bits (1337), Expect = 2.1e-147
Identity = 266/394 (67.51%), Postives = 316/394 (80.20%), Query Frame = 0

Query: 6   SEMKDHSSATLKAPSLGVNVPS--RSLQAHRSEGEILLSPNLKAFTFSELKNATKNFRPD 65
           S +   +S +    SL +N  S   SL   R+EGEIL SPNLKAFTF+ELKNAT+NFRPD
Sbjct: 27  SRVSSKTSRSTVPSSLSINSYSSVESLPTPRTEGEILSSPNLKAFTFNELKNATRNFRPD 86

Query: 66  SLIGEGGFGYVYKGWIDEHTLEAVRPGAGMVIAVKKLKPEGFQGHKEWLSEVNYLGQLHH 125
           SL+GEGGFGYV+KGWID  TL A +PG+G+V+AVKKLK EG+QGHKEWL+EVNYLGQL H
Sbjct: 87  SLLGEGGFGYVFKGWIDGTTLTASKPGSGIVVAVKKLKTEGYQGHKEWLTEVNYLGQLSH 146

Query: 126 SNLVKLIGFCLDGDSRLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGLSFLH 185
            NLVKL+G+C++G++RLLVYE+M KGSLENHLFRRGA+PL+WAIR+KVAIGAA+GL+FLH
Sbjct: 147 PNLVKLVGYCVEGENRLLVYEFMPKGSLENHLFRRGAQPLTWAIRMKVAIGAAKGLTFLH 206

Query: 186 NSEEPVIYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPEYIA 245
           +++  VIYRDFKA+NILLD+EFN+KLSDFGLAKAGPTGD+THVSTQVMGT GYAAPEY+A
Sbjct: 207 DAKSQVIYRDFKAANILLDAEFNSKLSDFGLAKAGPTGDKTHVSTQVMGTHGYAAPEYVA 266

Query: 246 TGRLTARCDVYSFGVVLLELLSGRRAVDKTKVGVEQHLVDWAKPYLADKRKLFRIMDTKL 305
           TGRLTA+ DVYSFGVVLLELLSGRRAVDK+KVG+EQ LVDWA PYL DKRKLFRIMDT+L
Sbjct: 267 TGRLTAKSDVYSFGVVLLELLSGRRAVDKSKVGMEQSLVDWATPYLGDKRKLFRIMDTRL 326

Query: 306 EGQYPQRAAYMTTILALQCIR-EAKFRPQMSEVLSALEQLPLVKHADNQRERE-RAPSPG 365
            GQYPQ+ AY    LALQC+  +AK RP+MSEVL+ L+QL   K       R+ +  SP 
Sbjct: 327 GGQYPQKGAYTAASLALQCLNPDAKLRPKMSEVLAKLDQLESTKPGTGVGNRQAQIDSPR 386

Query: 366 -------PKSPLRHKYPSPVNSTASKGGGSPLPS 389
                   KSP R+ Y  P+       G SPLP+
Sbjct: 387 GSNGSIVQKSPRRYSYDRPLLHITP--GASPLPT 418

BLAST of MC03g1115 vs. TAIR 10
Match: AT2G02800.2 (protein kinase 2B )

HSP 1 Score: 519.6 bits (1337), Expect = 2.1e-147
Identity = 266/394 (67.51%), Postives = 316/394 (80.20%), Query Frame = 0

Query: 6   SEMKDHSSATLKAPSLGVNVPS--RSLQAHRSEGEILLSPNLKAFTFSELKNATKNFRPD 65
           S +   +S +    SL +N  S   SL   R+EGEIL SPNLKAFTF+ELKNAT+NFRPD
Sbjct: 27  SRVSSKTSRSTVPSSLSINSYSSVESLPTPRTEGEILSSPNLKAFTFNELKNATRNFRPD 86

Query: 66  SLIGEGGFGYVYKGWIDEHTLEAVRPGAGMVIAVKKLKPEGFQGHKEWLSEVNYLGQLHH 125
           SL+GEGGFGYV+KGWID  TL A +PG+G+V+AVKKLK EG+QGHKEWL+EVNYLGQL H
Sbjct: 87  SLLGEGGFGYVFKGWIDGTTLTASKPGSGIVVAVKKLKTEGYQGHKEWLTEVNYLGQLSH 146

Query: 126 SNLVKLIGFCLDGDSRLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGLSFLH 185
            NLVKL+G+C++G++RLLVYE+M KGSLENHLFRRGA+PL+WAIR+KVAIGAA+GL+FLH
Sbjct: 147 PNLVKLVGYCVEGENRLLVYEFMPKGSLENHLFRRGAQPLTWAIRMKVAIGAAKGLTFLH 206

Query: 186 NSEEPVIYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPEYIA 245
           +++  VIYRDFKA+NILLD+EFN+KLSDFGLAKAGPTGD+THVSTQVMGT GYAAPEY+A
Sbjct: 207 DAKSQVIYRDFKAANILLDAEFNSKLSDFGLAKAGPTGDKTHVSTQVMGTHGYAAPEYVA 266

Query: 246 TGRLTARCDVYSFGVVLLELLSGRRAVDKTKVGVEQHLVDWAKPYLADKRKLFRIMDTKL 305
           TGRLTA+ DVYSFGVVLLELLSGRRAVDK+KVG+EQ LVDWA PYL DKRKLFRIMDT+L
Sbjct: 267 TGRLTAKSDVYSFGVVLLELLSGRRAVDKSKVGMEQSLVDWATPYLGDKRKLFRIMDTRL 326

Query: 306 EGQYPQRAAYMTTILALQCIR-EAKFRPQMSEVLSALEQLPLVKHADNQRERE-RAPSPG 365
            GQYPQ+ AY    LALQC+  +AK RP+MSEVL+ L+QL   K       R+ +  SP 
Sbjct: 327 GGQYPQKGAYTAASLALQCLNPDAKLRPKMSEVLAKLDQLESTKPGTGVGNRQAQIDSPR 386

Query: 366 -------PKSPLRHKYPSPVNSTASKGGGSPLPS 389
                   KSP R+ Y  P+       G SPLP+
Sbjct: 387 GSNGSIVQKSPRRYSYDRPLLHITP--GASPLPT 418

BLAST of MC03g1115 vs. TAIR 10
Match: AT1G26970.1 (Protein kinase superfamily protein )

HSP 1 Score: 503.1 bits (1294), Expect = 2.1e-142
Identity = 252/343 (73.47%), Postives = 287/343 (83.67%), Query Frame = 0

Query: 4   KKSEMKDHSSATLKAPSLGVNVPSRSLQAHRSEGEILLSPNLKAFTFSELKNATKNFRPD 63
           K+S+    SS T+++ S   +    SLQ  RSEGE+L SP LKAFTF+ELK AT+NFRPD
Sbjct: 27  KRSQSSRLSSLTIQSSSYNDDTSVASLQTPRSEGELLASPTLKAFTFNELKTATRNFRPD 86

Query: 64  SLIGEGGFGYVYKGWIDEHTLEAVRPGAGMVIAVKKLKPEGFQGHKEWLSEVNYLGQLHH 123
           S+IGEGGFGYVYKGWIDE TL   +PG+GMV+AVKKLK EGFQGH++WL+EV+ LG+LHH
Sbjct: 87  SVIGEGGFGYVYKGWIDERTLSPSKPGSGMVVAVKKLKEEGFQGHRQWLAEVDCLGRLHH 146

Query: 124 SNLVKLIGFCLDGDS-RLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGLSFL 183
            NLVKLIG+C  GD  RLLVYEYM KGSLENHLFRRGA P+ W  RIKVAIGAARGL+FL
Sbjct: 147 MNLVKLIGYCSKGDHIRLLVYEYMPKGSLENHLFRRGAEPIPWRTRIKVAIGAARGLAFL 206

Query: 184 HNSEEPVIYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPEYI 243
           H  E  VIYRDFKASNILLDSEFNAKLSDFGLAK GPTGDRTHVSTQVMGTQGYAAPEY+
Sbjct: 207 H--EAQVIYRDFKASNILLDSEFNAKLSDFGLAKVGPTGDRTHVSTQVMGTQGYAAPEYV 266

Query: 244 ATGRLTARCDVYSFGVVLLELLSGRRAVDKTKVGVEQHLVDWAKPYLADKRKLFRIMDTK 303
           ATGR+TA+ DVYSFGVVLLELLSGR  VDKTKVGVE++LVDWA PYL DKRK+FRIMDTK
Sbjct: 267 ATGRITAKSDVYSFGVVLLELLSGRLTVDKTKVGVERNLVDWAIPYLGDKRKVFRIMDTK 326

Query: 304 LEGQYPQRAAYMTTILALQCI-REAKFRPQMSEVLSALEQLPL 345
           L GQYP + A +T   ALQC+ +E K RP+MS+VLS LE+L +
Sbjct: 327 LGGQYPHKGACLTANTALQCLNQEPKLRPKMSDVLSTLEELEM 367

BLAST of MC03g1115 vs. TAIR 10
Match: AT1G14370.1 (protein kinase 2A )

HSP 1 Score: 499.2 bits (1284), Expect = 3.0e-141
Identity = 249/361 (68.98%), Postives = 295/361 (81.72%), Query Frame = 0

Query: 34  RSEGEILLSPNLKAFTFSELKNATKNFRPDSLIGEGGFGYVYKGWIDEHTLEAVRPGAGM 93
           R+EGEIL SPNLKAFTF+ELKNATKNFR D+L+GEGGFG V+KGWID+ +L A RPG+G+
Sbjct: 60  RTEGEILSSPNLKAFTFNELKNATKNFRQDNLLGEGGFGCVFKGWIDQTSLTASRPGSGI 119

Query: 94  VIAVKKLKPEGFQGHKEWLSEVNYLGQLHHSNLVKLIGFCLDGDSRLLVYEYMSKGSLEN 153
           V+AVK+LKPEGFQGHKEWL+EVNYLGQL H NLV L+G+C +G++RLLVYE+M KGSLEN
Sbjct: 120 VVAVKQLKPEGFQGHKEWLTEVNYLGQLSHPNLVLLVGYCAEGENRLLVYEFMPKGSLEN 179

Query: 154 HLFRRGARPLSWAIRIKVAIGAARGLSFLHNSEEPVIYRDFKASNILLDSEFNAKLSDFG 213
           HLFRRGA+PL+WAIR+KVA+GAA+GL+FLH ++  VIYRDFKA+NILLD++FNAKLSDFG
Sbjct: 180 HLFRRGAQPLTWAIRMKVAVGAAKGLTFLHEAKSQVIYRDFKAANILLDADFNAKLSDFG 239

Query: 214 LAKAGPTGDRTHVSTQVMGTQGYAAPEYIATGRLTARCDVYSFGVVLLELLSGRRAVDKT 273
           LAKAGPTGD THVST+V+GT GYAAPEY+ATGRLTA+ DVYSFGVVLLEL+SGRRA+D +
Sbjct: 240 LAKAGPTGDNTHVSTKVIGTHGYAAPEYVATGRLTAKSDVYSFGVVLLELISGRRAMDNS 299

Query: 274 KVGVEQHLVDWAKPYLADKRKLFRIMDTKLEGQYPQRAAYMTTILALQCIR-EAKFRPQM 333
             G E  LVDWA PYL DKRKLFRIMDTKL GQYPQ+ A+    LALQC+  +AK RP+M
Sbjct: 300 NGGNEYSLVDWATPYLGDKRKLFRIMDTKLGGQYPQKGAFTAANLALQCLNPDAKLRPKM 359

Query: 334 SEVLSALEQL-----PLVKHADNQRERERAPSPGPKSPLRHKYPSPVNSTASKGGGSPLP 389
           SEVL  LEQL     P  KH   +  R    S   KSP+R+ +  P+       G SPLP
Sbjct: 360 SEVLVTLEQLESVAKPGTKHTQMESPRFHHSSVMQKSPVRYSHDRPLLHMTP--GASPLP 418

BLAST of MC03g1115 vs. TAIR 10
Match: AT1G69790.1 (Protein kinase superfamily protein )

HSP 1 Score: 476.9 bits (1226), Expect = 1.6e-134
Identity = 231/310 (74.52%), Postives = 267/310 (86.13%), Query Frame = 0

Query: 34  RSEGEILLSPNLKAFTFSELKNATKNFRPDSLIGEGGFGYVYKGWIDEHTLEAVRPGAGM 93
           RSEGE+L SP LKAFTF+ELK AT+NF+P+S+IGEGGFG VYKGWI E +L   +PG+GM
Sbjct: 58  RSEGELLPSPTLKAFTFNELKTATRNFKPNSMIGEGGFGCVYKGWIGERSLSPSKPGSGM 117

Query: 94  VIAVKKLKPEGFQGHKEWLSEVNYLGQLHHSNLVKLIGFCLDGDSRLLVYEYMSKGSLEN 153
           V+AVKKLK EGFQGHKEWL+EV+YLG+LHH NLVKLIG+CL+G+ RLLVYEYM KGSLEN
Sbjct: 118 VVAVKKLKSEGFQGHKEWLTEVHYLGRLHHMNLVKLIGYCLEGEKRLLVYEYMPKGSLEN 177

Query: 154 HLFRRGARPLSWAIRIKVAIGAARGLSFLHNSEEPVIYRDFKASNILLDSEFNAKLSDFG 213
           HLFRRGA P+ W  R+KVA  AARGLSFLH  E  VIYRDFKASNILLD +FNAKLSDFG
Sbjct: 178 HLFRRGAEPIPWKTRMKVAFSAARGLSFLH--EAKVIYRDFKASNILLDVDFNAKLSDFG 237

Query: 214 LAKAGPTGDRTHVSTQVMGTQGYAAPEYIATGRLTARCDVYSFGVVLLELLSGRRAVDKT 273
           LAKAGPTGDRTHV+TQV+GTQGYAAPEYIATGRLT++ DVYSFGVVLLELLSGR  +DK+
Sbjct: 238 LAKAGPTGDRTHVTTQVIGTQGYAAPEYIATGRLTSKSDVYSFGVVLLELLSGRPTLDKS 297

Query: 274 KVGVEQHLVDWAKPYLADKRKLFRIMDTKLEGQYPQRAAYMTTILALQCIR-EAKFRPQM 333
           KVGVE++LVDWA PYL D+RK+FRIMDTKL GQYP + A     +AL+C+  E K RP M
Sbjct: 298 KVGVERNLVDWAIPYLVDRRKVFRIMDTKLGGQYPHKGACAAANIALRCLNTEPKLRPDM 357

Query: 334 SEVLSALEQL 343
           ++VLS L+QL
Sbjct: 358 ADVLSTLQQL 365

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O498403.0e-14667.51Probable serine/threonine-protein kinase PBL3 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q5PP292.9e-14173.47Probable serine/threonine-protein kinase PBL4 OS=Arabidopsis thaliana OX=3702 GN... [more]
O498394.2e-14068.98Probable serine/threonine-protein kinase PBL2 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q5XF792.2e-13374.52Probable serine/threonine-protein kinase PBL18 OS=Arabidopsis thaliana OX=3702 G... [more]
P465737.7e-12666.07Probable serine/threonine-protein kinase PBL10 OS=Arabidopsis thaliana OX=3702 G... [more]
Match NameE-valueIdentityDescription
XP_022137441.12.25e-282100.00probable serine/threonine-protein kinase PBL3 [Momordica charantia][more]
XP_008437450.16.77e-25189.95PREDICTED: protein kinase 2B, chloroplastic-like [Cucumis melo][more]
XP_038895448.11.69e-25090.46probable serine/threonine-protein kinase PBL3 isoform X1 [Benincasa hispida][more]
XP_011651142.12.63e-24889.18probable serine/threonine-protein kinase PBL3 isoform X1 [Cucumis sativus] >KAE8... [more]
XP_031745128.11.03e-24789.15probable serine/threonine-protein kinase PBL3 isoform X2 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
A0A6J1C6M01.09e-282100.00probable serine/threonine-protein kinase PBL3 OS=Momordica charantia OX=3673 GN=... [more]
A0A1S3AU613.28e-25189.95protein kinase 2B, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103482862 PE... [more]
A0A6J1E7M06.82e-24489.95probable serine/threonine-protein kinase PBL3 isoform X1 OS=Cucurbita moschata O... [more]
A0A6J1KLE71.95e-24389.68probable serine/threonine-protein kinase PBL3 isoform X1 OS=Cucurbita maxima OX=... [more]
A0A6J1GSG83.83e-22982.99probable serine/threonine-protein kinase PBL3 OS=Cucurbita moschata OX=3662 GN=L... [more]
Match NameE-valueIdentityDescription
AT2G02800.12.1e-14767.51protein kinase 2B [more]
AT2G02800.22.1e-14767.51protein kinase 2B [more]
AT1G26970.12.1e-14273.47Protein kinase superfamily protein [more]
AT1G14370.13.0e-14168.98protein kinase 2A [more]
AT1G69790.11.6e-13474.52Protein kinase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 146..359
e-value: 8.8E-56
score: 190.5
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 35..145
e-value: 3.9E-33
score: 115.7
NoneNo IPR availablePIRSRPIRSR000620-1PIRSR000620-1coord: 66..264
e-value: 4.5E-18
score: 63.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 370..390
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 346..390
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..21
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 346..362
NoneNo IPR availablePANTHERPTHR45621:SF72BNAC02G17010D PROTEINcoord: 14..377
NoneNo IPR availablePANTHERPTHR45621OS01G0588500 PROTEIN-RELATEDcoord: 14..377
NoneNo IPR availableCDDcd14066STKc_IRAKcoord: 66..342
e-value: 1.69841E-91
score: 274.535
IPR001245Serine-threonine/tyrosine-protein kinase, catalytic domainPFAMPF07714PK_Tyr_Ser-Thrcoord: 64..337
e-value: 1.3E-43
score: 149.2
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 66..99
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 189..201
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 60..345
score: 36.668556
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 39..340

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC03g1115.1MC03g1115.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004672 protein kinase activity