MC03g0977 (gene) Bitter gourd (Dali-11) v1

BLAST of MC03g0977 vs. ExPASy Swiss-Prot
Match: A0A2P1GIW7 (Stemmadenine O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=SAT PE=1 SV=1)

HSP 1 Score: 277.3 bits (708), Expect = 2.9e-73
Identity = 168/428 (39.25%), Postives = 246/428 (57.48%), Query Frame = 0

Query: 1   MELKVEIISTESIKPSSPTPPEQRELKLSLLDQVAPGSYTPLLFFYPAGQLDRG-DHGGR 60
           M  +++I+S E I+PSSPTP   +  KLS LDQV    + P++ FYP  QLD   D   R
Sbjct: 1   MAPQMQILSEELIQPSSPTPQTLKTHKLSHLDQVLLTCHIPIILFYP-NQLDSNLDRAQR 60

Query: 61  CRKLKESLAETLTRFYLLAGTLVEAYLVECNDEGVEFSEARVACQLADVMDKPDDVAALH 120
              LK SL+  LT+FY LAG +     V+CND GV F EARV  QL++ + K   +  L+
Sbjct: 61  SENLKRSLSTVLTQFYPLAGRININSSVDCNDSGVPFLEARVHSQLSEAI-KNVAIDELN 120

Query: 121 RLLPFDP-----DAVLDRDCILAVRYNAFDCGGVVIALCVTHKIVDGTSATTFTKAWAAA 180
           + LPF P     ++ L +D  LAV+ + F+CGG  I +C++HKI D  S  TF  +W A 
Sbjct: 121 QYLPFQPYPGGEESGLKKDIPLAVKISCFECGGTAIGVCISHKIADALSLATFLNSWTAT 180

Query: 181 CRGDHELTVVPTFD-AAELFPPMEIRGGNKRQSRMDKIVTKRFIFSKSNIAALKQEANSD 240
           C+ + ++ V P FD  +  FPPME     +     + IV KRF+F K  + ALK +  S 
Sbjct: 181 CQEETDI-VQPNFDLGSHHFPPMESIPAPEFLPD-ENIVMKRFVFDKEKLEALKAQLASS 240

Query: 241 AFLNQRPPSRVETVSAFIWKRFMALARSKPTTTAAKRFAILHAVNIRNRLSPPLPPHSFG 300
           A    +  SRV+ V A IWK+F+ + R+K  T    +     AVN+R+R++PP P  + G
Sbjct: 241 A-TEVKNSSRVQIVIAVIWKQFIDVTRAKFDT--KNKLVAAQAVNLRSRMNPPFPQSAMG 300

Query: 301 NLWWFAVAEAPIEEEKDYPFLVSKIRNAITEIDGEYVTALQDAEKAMRAKMKMGQMVYSG 360
           N+   A A A  EE+KD+  LV  ++ ++ +ID E+V  LQ           +  + Y  
Sbjct: 301 NIATMAYAVA--EEDKDFSDLVGPLKTSLAKIDDEHVKELQKG---------VTYLDYEA 360

Query: 361 E-VELYSFTSWCSFPIYESDFGWGRPAWVCSPGRPYKNVVLLVNTGDGEGIEAWVNLEEK 420
           E  EL+SF+SWC    Y+ DFGWG+P  VC+   P KN+V L++T + +G+EAW+++ E 
Sbjct: 361 EPQELFSFSSWCRLGFYDLDFGWGKPVSVCTTTVPMKNLVYLMDTRNEDGMEAWISMAED 410

BLAST of MC03g0977 vs. ExPASy Swiss-Prot
Match: Q70PR7 (Vinorine synthase OS=Rauvolfia serpentina OX=4060 GN=ACT PE=1 SV=2)

HSP 1 Score: 258.1 bits (658), Expect = 1.8e-67
Identity = 159/438 (36.30%), Postives = 241/438 (55.02%), Query Frame = 0

Query: 1   MELKVEIISTESIKPSSPTPPEQRELKLSLLDQVAPGSYTPLLFFYPAGQLDRGDHGGRC 60
           M  ++E +S E I PSSPTP   +  K+S LDQ+    + P + FYP       D     
Sbjct: 1   MAPQMEKVSEELILPSSPTPQSLKCYKISHLDQLLLTCHIPFILFYPNPLDSNLDPAQTS 60

Query: 61  RKLKESLAETLTRFYLLAGTLVEAYLVECNDEGVEFSEARVACQLADVMDKPDDVAALHR 120
           + LK+SL++ LT FY LAG +     V+CND GV F EARV  QL+  +    +V  L +
Sbjct: 61  QHLKQSLSKVLTHFYPLAGRINVNSSVDCNDSGVPFVEARVQAQLSQAI---QNVVELEK 120

Query: 121 LLPFDPDAV-------LDRDCILAVRYNAFDCGGVVIALCVTHKIVDGTSATTFTKAWAA 180
           L  + P A        ++ D  LAV+ + F+CGG  I + ++HKI D  S  TF  AW A
Sbjct: 121 LDQYLPSAAYPGGKIEVNEDVPLAVKISFFECGGTAIGVNLSHKIADVLSLATFLNAWTA 180

Query: 181 ACRGDHELTVVPTFD-AAELFPPMEIRGGNKRQSRMDKIVTKRFIFSKSNIAALKQEANS 240
            CRG+ E+ V+P FD AA  FPP++    +      + +V KRF+F K  I AL+ +A+S
Sbjct: 181 TCRGETEI-VLPNFDLAARHFPPVD-NTPSPELVPDENVVMKRFVFDKEKIGALRAQASS 240

Query: 241 DAFLNQRPPSRVETVSAFIWKRFMALARSKPTTTAAKRFAILHAVNIRNRLSPPLPPHSF 300
            +   ++  SRV+ V A+IWK  + + R+K    A  +F ++ AVN+R+R++PPLP ++ 
Sbjct: 241 AS--EEKNFSRVQLVVAYIWKHVIDVTRAK--YGAKNKFVVVQAVNLRSRMNPPLPHYAM 300

Query: 301 GNLWWFAVAEAPIEEEKDYPFLVSKIRNAITEIDGEYVTALQDAEKAMRAKMKMGQMVYS 360
           GN+     A    E +KD+P L+  +R ++ + + ++   L          +K    +Y 
Sbjct: 301 GNIATLLFAAVDAEWDKDFPDLIGPLRTSLEKTEDDHNHEL----------LKGMTCLYE 360

Query: 361 GE-VELYSFTSWCSFPIYESDFGWGRPAWVCSPGRPYKNVVLLVNTGDGEGIEAWVNLEE 420
            E  EL SFTSWC    Y+ DFGWG+P   C+   P +N  LL++T  G+G+EAW+ + E
Sbjct: 361 LEPQELLSFTSWCRLGFYDLDFGWGKPLSACTTTFPKRNAALLMDTRSGDGVEAWLPMAE 417

Query: 421 KDMPVFESDPELLAFTSS 430
            +M +     ELL+   S
Sbjct: 421 DEMAML--PVELLSLVDS 417

BLAST of MC03g0977 vs. ExPASy Swiss-Prot
Match: I3PLR4 ((13S,14R)-1,13-dihydroxy-N-methylcanadine 13-O-acetyltransferase AT1 OS=Papaver somniferum OX=3469 GN=AT1 PE=1 SV=1)

HSP 1 Score: 233.4 bits (594), Expect = 4.8e-60
Identity = 157/463 (33.91%), Postives = 229/463 (49.46%), Query Frame = 0

Query: 5   VEIISTESIKPSSPTPPEQRELKLSLLDQVAPGSYTPLLFFYPAGQLDRGDHGGR----C 64
           VE+IS E+IKP +PTP + R   +SLLDQ +   Y P++ FYPA   D    G +     
Sbjct: 9   VEVISKETIKPRNPTPYQLRNYNMSLLDQYSSLVYVPIILFYPAAS-DANSTGSKHHDDL 68

Query: 65  RKLKESLAETLTRFYLLAGTLVEAYLVECNDEGVEFSEARVACQLADVMDKPDDVAALHR 124
             LK SL+ETL  FY +AG + +   V+CNDEG++F E R+  ++ D M K D  A L  
Sbjct: 69  HLLKRSLSETLVHFYPMAGRMKDNMTVDCNDEGIDFFEVRIKGRMCDFMMKSD--AHLSL 128

Query: 125 LLPFDPDAV-LDRDCILAVRYNAFDCGGVVIALCVTHKIVDGTSATTFTKAWAA----AC 184
           LLP +  +    ++  + V+ N FDCGG  I  C+++KI D  +  TF ++ A     A 
Sbjct: 129 LLPSEVASTNFVKEAQVIVQVNMFDCGGTAICFCISNKIADACTMITFIRSLAGTTNIAR 188

Query: 185 RGDH------ELTVVPTFDAAELFPPME-----IRGGNKRQSRMDKIVTKRFIFSKSNIA 244
           RG           +VP+FD+  LFPP E     +    +  + +DK+V+KRF+F  + I 
Sbjct: 189 RGSSIAAPTTNQNLVPSFDSTSLFPPSEQLASQVSYPTQDSTSVDKLVSKRFVFDAAKIT 248

Query: 245 ALKQEANS---DAFLNQRPPSRVETVSAFIWKRFMALARSKPTTTAAKRFAILHAVNIRN 304
           + +++  S   D +   R P+RVE VSA IWK   +  +S P  + +    + HA+N R 
Sbjct: 249 SAREKLQSLMHDKYKCHR-PTRVEVVSALIWK---SAVKSAPPGSIS---TVTHAMNFRK 308

Query: 305 RLSPPLPPHSFGNLWWFAVAEAPIEEEKDYPFLVSKIRN-------AITEIDGEYVTALQ 364
           ++ PPL   SFGNL     A  P            K+ +       A+ E+        +
Sbjct: 309 KMDPPLQDASFGNLCVVVTAVLPATTATTTNPATKKVSSTSNEEQVALDELSDFVALLRR 368

Query: 365 DAEKAMRAKMKMGQMV----------------YSGEVELYSFTSWCSFPIYESDFGWGRP 419
           + +K    K  M +++                   +V     TSWC F  YE+DFGWG P
Sbjct: 369 EIDKVKGDKGCMEKIIQKFIYGHDASVAKDSDVEDKVTALFMTSWCKFGFYEADFGWGTP 428

BLAST of MC03g0977 vs. ExPASy Swiss-Prot
Match: Q94FT4 (Salutaridinol 7-O-acetyltransferase OS=Papaver somniferum OX=3469 GN=SALAT PE=1 SV=1)

HSP 1 Score: 228.4 bits (581), Expect = 1.5e-58
Identity = 154/466 (33.05%), Postives = 238/466 (51.07%), Query Frame = 0

Query: 5   VEIISTESIKPSSPTPPEQRELKLSLLDQVAP-GSYTPLLFFYP---AGQLDRGDHGGRC 64
           VE+IS E+IKP++PTP + +   LSLLDQ  P   Y P++ FYP   A      +H    
Sbjct: 9   VEVISKETIKPTTPTPSQLKNFNLSLLDQCFPLYYYVPIILFYPATAANSTGSSNHHDDL 68

Query: 65  RKLKESLAETLTRFYLLAGTLVEAYLVECNDEGVEFSEARVACQLADVMDKPDDVAALHR 124
             LK SL++TL  FY +AG +++  LV+C+D+G+ F + ++  ++ + M +PD    L +
Sbjct: 69  DLLKSSLSKTLVHFYPMAGRMIDNILVDCHDQGINFYKVKIRGKMCEFMSQPD--VPLSQ 128

Query: 125 LLPFD-PDAVLDRDCILAVRYNAFDCGGVVIALCVTHKIVDGTSATTFTKAWAAACRGDH 184
           LLP +   A + ++ ++ V+ N FDCGG  I   V+HKI D  + +TF ++WA+  +   
Sbjct: 129 LLPSEVVSASVPKEALVIVQVNMFDCGGTAICSSVSHKIADAATMSTFIRSWASTTKTSR 188

Query: 185 ---------ELTVVPTFDAAELFPPMEIRGGNKRQSRM-----------DKIVTKRFIFS 244
                    +  ++P+FD+A LFPP E        S +           DK V+KRF+F 
Sbjct: 189 SGGSTAAVTDQKLIPSFDSASLFPPSERLTSPSGMSEIPFSSTPEDTEDDKTVSKRFVFD 248

Query: 245 KSNIAALKQEAN---SDAFLNQRPPSRVETVSAFIWKRFMALARSKPTTTAAKRFAILHA 304
            + I +++++      D + ++R  +RVE V++ IWK  M       +T A     + HA
Sbjct: 249 FAKITSVREKLQVLMHDNYKSRR-QTRVEVVTSLIWKSVM------KSTPAGFLPVVHHA 308

Query: 305 VNIRNRLSPPLPPHSFGNLWWFAVAEAPIEEEKDYPFLVSKIRNAITEIDGEYVTALQDA 364
           VN+R ++ PPL   SFGNL     A  P          V+K  N+ +      +  L D 
Sbjct: 309 VNLRKKMDPPLQDVSFGNLSVTVSAFLPATTTTT-TNAVNKTINSTSSESQVVLHELHDF 368

Query: 365 EKAMRAKM-----------KMGQMVYSG---------EVELYSF--TSWCSFPIYESDFG 419
              MR+++           K+ Q   SG         +VE+ +F  +SWC   +YE DFG
Sbjct: 369 IAQMRSEIDKVKGDKGSLEKVIQNFASGHDASIKKINDVEVINFWISSWCRMGLYEIDFG 428

BLAST of MC03g0977 vs. ExPASy Swiss-Prot
Match: A0A2K8FQU5 (Tabersonine-19-hydroxy-O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=TAT PE=1 SV=1)

HSP 1 Score: 224.6 bits (571), Expect = 2.2e-57
Identity = 151/427 (35.36%), Postives = 223/427 (52.22%), Query Frame = 0

Query: 5   VEIISTESIKPSSPTPPEQRELKLSLLDQVAPGSYTPLLFFYPAGQLDRGDHGGRCRKLK 64
           VEI+S   IKPSSPTP   R   LS +DQ      T L FFY           G    LK
Sbjct: 6   VEIVSKMFIKPSSPTPQSLRRYNLSSIDQTIDSEVTSLAFFYTYNPSHESSKIGDL--LK 65

Query: 65  ESLAETLTRFYLLAGTLVEAYLVECNDEGVEFSEARVACQLADVMDKPDDVAALHRLLPF 124
            SL++TL  +Y  AG L+E   ++CNDEGVEF E R+  ++ D++ +    A    +LP 
Sbjct: 66  NSLSKTLVSYYQFAGRLIENDYIDCNDEGVEFVEVRIHGRMNDILKRGKSFAT-DLVLPT 125

Query: 125 DPDAVLDRDCILAVRYNAFDCGGVVIALCVTHKIVDGTSATTFTKAWAAACRGDHELTVV 184
              A L  D +L V+ + FDCGG+ I    +HK+ DG S   F K WA++          
Sbjct: 126 RIIA-LHEDSLLIVQLSHFDCGGIAIGFGASHKVSDGVSNVMFMKDWASSTSLSTFHKPT 185

Query: 185 PTFDAAELFPPMEIR-GGNKRQSRMDKIVTKRFIFSKSNIAALKQEANSDAFLNQRPPSR 244
           P   A  +FPP + +   NK      + + KRF+FS   I  LK +A        + PSR
Sbjct: 186 PLLTADSIFPPEDNKLLSNKSIVSFQQCLGKRFVFSTEAIEKLKSKAIEYGI---QKPSR 245

Query: 245 VETVSAFIWK--RFMALARSKPTTTAAKRFAILHAVNIRNRLSPPLPPHSFGNLWWFAVA 304
           VE V+AF+ +      L R KP       +AI+ AVN+R  L+  LP +S GN++ F   
Sbjct: 246 VEVVTAFLCQCAANCDLPRKKP-------YAIISAVNLRPYLA--LPQNSIGNIFSFYFC 305

Query: 305 EAPIEEEKDYPFLVSKIRNAITEIDGEYVTALQ-DAEKAMRAKMKMGQMVYSGEVELYSF 364
                 +  +  L+SK+RN   ++    ++  +   E  M+   K    +    ++ Y  
Sbjct: 306 INDEGMDNQFSALISKLRNGKQKLLENIISKEKLTYESQMQELTKCLDQLNISSLDTYFC 365

Query: 365 TSWCSFPIYESDFGWGRPAWVCSPGRPY-KNVVLLVNTGDGEGIEAWVNLEEKDMPVFES 424
           +SWC FP+Y+ DFGWG+P  V SP +P+ K+++LL+++ +G+GIEA + +EEK M  FE 
Sbjct: 366 SSWCRFPVYDIDFGWGKPILV-SPFQPHVKDLILLMDSPEGDGIEALITMEEKKMAAFEK 415

Query: 425 DPELLAF 427
           + EL +F
Sbjct: 426 NEELRSF 415

BLAST of MC03g0977 vs. NCBI nr
Match: XP_022137523.1 (vinorine synthase-like [Momordica charantia])

HSP 1 Score: 871 bits (2250), Expect = 0.0
Identity = 431/431 (100.00%), Postives = 431/431 (100.00%), Query Frame = 0

Query: 1   MELKVEIISTESIKPSSPTPPEQRELKLSLLDQVAPGSYTPLLFFYPAGQLDRGDHGGRC 60
           MELKVEIISTESIKPSSPTPPEQRELKLSLLDQVAPGSYTPLLFFYPAGQLDRGDHGGRC
Sbjct: 1   MELKVEIISTESIKPSSPTPPEQRELKLSLLDQVAPGSYTPLLFFYPAGQLDRGDHGGRC 60

Query: 61  RKLKESLAETLTRFYLLAGTLVEAYLVECNDEGVEFSEARVACQLADVMDKPDDVAALHR 120
           RKLKESLAETLTRFYLLAGTLVEAYLVECNDEGVEFSEARVACQLADVMDKPDDVAALHR
Sbjct: 61  RKLKESLAETLTRFYLLAGTLVEAYLVECNDEGVEFSEARVACQLADVMDKPDDVAALHR 120

Query: 121 LLPFDPDAVLDRDCILAVRYNAFDCGGVVIALCVTHKIVDGTSATTFTKAWAAACRGDHE 180
           LLPFDPDAVLDRDCILAVRYNAFDCGGVVIALCVTHKIVDGTSATTFTKAWAAACRGDHE
Sbjct: 121 LLPFDPDAVLDRDCILAVRYNAFDCGGVVIALCVTHKIVDGTSATTFTKAWAAACRGDHE 180

Query: 181 LTVVPTFDAAELFPPMEIRGGNKRQSRMDKIVTKRFIFSKSNIAALKQEANSDAFLNQRP 240
           LTVVPTFDAAELFPPMEIRGGNKRQSRMDKIVTKRFIFSKSNIAALKQEANSDAFLNQRP
Sbjct: 181 LTVVPTFDAAELFPPMEIRGGNKRQSRMDKIVTKRFIFSKSNIAALKQEANSDAFLNQRP 240

Query: 241 PSRVETVSAFIWKRFMALARSKPTTTAAKRFAILHAVNIRNRLSPPLPPHSFGNLWWFAV 300
           PSRVETVSAFIWKRFMALARSKPTTTAAKRFAILHAVNIRNRLSPPLPPHSFGNLWWFAV
Sbjct: 241 PSRVETVSAFIWKRFMALARSKPTTTAAKRFAILHAVNIRNRLSPPLPPHSFGNLWWFAV 300

Query: 301 AEAPIEEEKDYPFLVSKIRNAITEIDGEYVTALQDAEKAMRAKMKMGQMVYSGEVELYSF 360
           AEAPIEEEKDYPFLVSKIRNAITEIDGEYVTALQDAEKAMRAKMKMGQMVYSGEVELYSF
Sbjct: 301 AEAPIEEEKDYPFLVSKIRNAITEIDGEYVTALQDAEKAMRAKMKMGQMVYSGEVELYSF 360

Query: 361 TSWCSFPIYESDFGWGRPAWVCSPGRPYKNVVLLVNTGDGEGIEAWVNLEEKDMPVFESD 420
           TSWCSFPIYESDFGWGRPAWVCSPGRPYKNVVLLVNTGDGEGIEAWVNLEEKDMPVFESD
Sbjct: 361 TSWCSFPIYESDFGWGRPAWVCSPGRPYKNVVLLVNTGDGEGIEAWVNLEEKDMPVFESD 420

Query: 421 PELLAFTSSSS 431
           PELLAFTSSSS
Sbjct: 421 PELLAFTSSSS 431

BLAST of MC03g0977 vs. NCBI nr
Match: XP_023520398.1 (vinorine synthase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 697 bits (1798), Expect = 3.28e-250
Identity = 339/429 (79.02%), Postives = 382/429 (89.04%), Query Frame = 0

Query: 3   LKVEIISTESIKPSSPTPPEQRELKLSLLDQVAPGSYTPLLFFYPAGQLDRGD-HGGRCR 62
           L VEIISTESIKPSSPTP E RELKLS LDQVAP SYTPLLFFY A QL  GD H  RCR
Sbjct: 6   LNVEIISTESIKPSSPTPSEHRELKLSFLDQVAPSSYTPLLFFYTADQLHGGDDHRERCR 65

Query: 63  KLKESLAETLTRFYLLAGTLVEAYLVECNDEGVEFSEARVACQLADVMDKPDDVAALHRL 122
           KLKESLAETL RFYLLAGTLVEAYLV+CND+GV+FSEARV+ ++++VM+KP DV +L RL
Sbjct: 66  KLKESLAETLNRFYLLAGTLVEAYLVDCNDDGVDFSEARVSGRMSEVMEKPHDVVSLLRL 125

Query: 123 LPFDPDAVLDRDCILAVRYNAFDCGGVVIALCVTHKIVDGTSATTFTKAWAAACRGDHEL 182
           LPFD DAVL+R+CILAVRYN F+CGGVV+ALCVTHKIVDGTSAT FTKAWAAACRGD + 
Sbjct: 126 LPFDHDAVLERECILAVRYNVFECGGVVVALCVTHKIVDGTSATMFTKAWAAACRGDDKY 185

Query: 183 TVVPTFDAAELFPPMEIRGGNKRQSRMDKIVTKRFIFSKSNIAALKQEANSDAFLNQRPP 242
            VVPTFDA ELFPPMEIRGGN+RQSRMDKIVT+RFIF+KSNIAALK +ANS+AFLNQRPP
Sbjct: 186 PVVPTFDATELFPPMEIRGGNRRQSRMDKIVTRRFIFNKSNIAALKNQANSEAFLNQRPP 245

Query: 243 SRVETVSAFIWKRFMALARSKPTTTAAKRFAILHAVNIRNRLSPPLPPHSFGNLWWFAVA 302
           SRVETVS F+WKRFMA+A+ KP T A KRFA++ AVN+RNR++PPLPPHSFGNLWWFA A
Sbjct: 246 SRVETVSGFLWKRFMAIAQQKPPTKA-KRFAVIQAVNLRNRMNPPLPPHSFGNLWWFATA 305

Query: 303 EAPIEEEKDYPFLVSKIRNAITEIDGEYVTALQDAEKAMRAKMKMGQMVYSGEVELYSFT 362
           +API+EE+D+P LV+KIR AI EI+G+Y+  LQD EK+M+AKMKMGQMVYSGE+ELYSFT
Sbjct: 306 DAPIDEEQDFPSLVAKIREAIKEINGDYIKTLQDTEKSMKAKMKMGQMVYSGELELYSFT 365

Query: 363 SWCSFPIYESDFGWGRPAWVCSPGRPYKNVVLLVNTGDGEGIEAWVNLEEKDMPVFESDP 422
           SWCSFP+YESDFGWG+PAWVCSPGRPYKNVVL VNT DGEGIEAWVNL E DM  FE+D 
Sbjct: 366 SWCSFPLYESDFGWGKPAWVCSPGRPYKNVVLFVNTSDGEGIEAWVNLTETDMKDFETDS 425

Query: 423 ELLAFTSSS 430
           ELL+F S S
Sbjct: 426 ELLSFASLS 433

BLAST of MC03g0977 vs. NCBI nr
Match: XP_023000804.1 (vinorine synthase-like [Cucurbita maxima])

HSP 1 Score: 696 bits (1796), Expect = 6.62e-250
Identity = 339/429 (79.02%), Postives = 381/429 (88.81%), Query Frame = 0

Query: 3   LKVEIISTESIKPSSPTPPEQRELKLSLLDQVAPGSYTPLLFFYPAGQLDRGD-HGGRCR 62
           L VEIISTESIKPSSPTP E RELKLS LDQVAP SYTPLLFFY A QL  GD H  RCR
Sbjct: 6   LNVEIISTESIKPSSPTPSEHRELKLSFLDQVAPSSYTPLLFFYTADQLHGGDDHRERCR 65

Query: 63  KLKESLAETLTRFYLLAGTLVEAYLVECNDEGVEFSEARVACQLADVMDKPDDVAALHRL 122
           KLKESLAETL RFYLLAGTLVEAYLV+CND+GVEFSE RV+ ++++VM+KP DV +L RL
Sbjct: 66  KLKESLAETLNRFYLLAGTLVEAYLVDCNDDGVEFSETRVSARMSEVMEKPHDVVSLLRL 125

Query: 123 LPFDPDAVLDRDCILAVRYNAFDCGGVVIALCVTHKIVDGTSATTFTKAWAAACRGDHEL 182
           LPFD DAVL+R+CILAVRYN F+CGGVV+ALCVTHKIVDGTSAT FTKAWAAACRGD + 
Sbjct: 126 LPFDHDAVLERECILAVRYNVFECGGVVVALCVTHKIVDGTSATMFTKAWAAACRGDDKY 185

Query: 183 TVVPTFDAAELFPPMEIRGGNKRQSRMDKIVTKRFIFSKSNIAALKQEANSDAFLNQRPP 242
            VVPTF+A ELFPPMEIRGGN+RQSRMDKIVT+RFIF+KSNIAALK +ANS+AFLNQRPP
Sbjct: 186 PVVPTFNATELFPPMEIRGGNRRQSRMDKIVTRRFIFNKSNIAALKNQANSEAFLNQRPP 245

Query: 243 SRVETVSAFIWKRFMALARSKPTTTAAKRFAILHAVNIRNRLSPPLPPHSFGNLWWFAVA 302
           SRVETVS F+WKRFMA+A+ KP T A KRFA++ AVN+RNR+ PPLPP+SFGNLWWFA A
Sbjct: 246 SRVETVSGFLWKRFMAIAQQKPPTKA-KRFAVIQAVNLRNRMDPPLPPNSFGNLWWFATA 305

Query: 303 EAPIEEEKDYPFLVSKIRNAITEIDGEYVTALQDAEKAMRAKMKMGQMVYSGEVELYSFT 362
           +API+EE+D+P LV+KIR AI EIDG+Y+  LQDAEK+M+AKMKMGQMVYSGE+ELYSFT
Sbjct: 306 DAPIDEEQDFPSLVAKIREAIKEIDGDYIKTLQDAEKSMKAKMKMGQMVYSGELELYSFT 365

Query: 363 SWCSFPIYESDFGWGRPAWVCSPGRPYKNVVLLVNTGDGEGIEAWVNLEEKDMPVFESDP 422
           SWCSFP+YESDFGWG+PAWVCSPGRPYKNVVL VNT DGEGIEAWVNL E DM  FE+D 
Sbjct: 366 SWCSFPLYESDFGWGKPAWVCSPGRPYKNVVLFVNTSDGEGIEAWVNLTETDMKDFETDS 425

Query: 423 ELLAFTSSS 430
           ELL+F S S
Sbjct: 426 ELLSFASLS 433

BLAST of MC03g0977 vs. NCBI nr
Match: XP_022923632.1 (vinorine synthase-like [Cucurbita moschata] >KAG6584134.1 Stemmadenine O-acetyltransferase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7019724.1 Vinorine synthase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 694 bits (1792), Expect = 2.69e-249
Identity = 338/429 (78.79%), Postives = 381/429 (88.81%), Query Frame = 0

Query: 3   LKVEIISTESIKPSSPTPPEQRELKLSLLDQVAPGSYTPLLFFYPAGQLDRGD-HGGRCR 62
           L VEIISTESIKPSSPTP E RELKLS LDQVAP SYTPLLFFY A QL  GD H  RCR
Sbjct: 6   LNVEIISTESIKPSSPTPSEHRELKLSFLDQVAPSSYTPLLFFYTADQLHGGDDHRERCR 65

Query: 63  KLKESLAETLTRFYLLAGTLVEAYLVECNDEGVEFSEARVACQLADVMDKPDDVAALHRL 122
           KLKESLAETL RFYLLAGTLVEAYLV+CND+GVEFSEARV+ ++++VM+KP DV +L RL
Sbjct: 66  KLKESLAETLNRFYLLAGTLVEAYLVDCNDDGVEFSEARVSGRMSEVMEKPHDVVSLLRL 125

Query: 123 LPFDPDAVLDRDCILAVRYNAFDCGGVVIALCVTHKIVDGTSATTFTKAWAAACRGDHEL 182
           LPFD DAVL+R+CILAVRYN F+CGGVV+ALCVTHKIVDGTSAT FTKAWAAACRGD + 
Sbjct: 126 LPFDHDAVLERECILAVRYNVFECGGVVVALCVTHKIVDGTSATMFTKAWAAACRGDDKY 185

Query: 183 TVVPTFDAAELFPPMEIRGGNKRQSRMDKIVTKRFIFSKSNIAALKQEANSDAFLNQRPP 242
            VVPTFDA ELFPPMEIRGGN+RQSRM+KIVT+RFIF+KSNIAALK +ANS+AFLNQRPP
Sbjct: 186 PVVPTFDATELFPPMEIRGGNRRQSRMEKIVTRRFIFNKSNIAALKHQANSEAFLNQRPP 245

Query: 243 SRVETVSAFIWKRFMALARSKPTTTAAKRFAILHAVNIRNRLSPPLPPHSFGNLWWFAVA 302
           SRVETVS F+WKRFMA+A+ KP T A KRFA++ AVN+RNR+ PPLPP+SFGNLWWFA A
Sbjct: 246 SRVETVSGFLWKRFMAIAQQKPPTKA-KRFAVIQAVNLRNRMDPPLPPNSFGNLWWFATA 305

Query: 303 EAPIEEEKDYPFLVSKIRNAITEIDGEYVTALQDAEKAMRAKMKMGQMVYSGEVELYSFT 362
           +API+EE+D+P LV+KIR AI EIDG+Y+  +QD EK+M+AKMKMGQMVYSGE+ELYSFT
Sbjct: 306 DAPIDEEQDFPSLVAKIREAIKEIDGDYIKTIQDTEKSMKAKMKMGQMVYSGELELYSFT 365

Query: 363 SWCSFPIYESDFGWGRPAWVCSPGRPYKNVVLLVNTGDGEGIEAWVNLEEKDMPVFESDP 422
           SWCSFP+YESDFGWG+PAWVCSPGRPYKNVVL VNT DGEGIEAWVNL E DM  FE+D 
Sbjct: 366 SWCSFPLYESDFGWGKPAWVCSPGRPYKNVVLFVNTSDGEGIEAWVNLTETDMKDFETDA 425

Query: 423 ELLAFTSSS 430
           ELL+F S S
Sbjct: 426 ELLSFASLS 433

BLAST of MC03g0977 vs. NCBI nr
Match: XP_038895261.1 (LOW QUALITY PROTEIN: stemmadenine O-acetyltransferase-like [Benincasa hispida])

HSP 1 Score: 676 bits (1743), Expect = 1.04e-241
Identity = 335/437 (76.66%), Postives = 374/437 (85.58%), Query Frame = 0

Query: 3   LKVEIISTESIKPSSPTPPEQRELKLSLLDQVAPGSYTPLLFFYPAGQLDRGD-HGGRCR 62
           LKVEIISTE IKPSSPTP + ++ KLSLLDQ AP SYTPLLFFYPA QL   D H  RCR
Sbjct: 6   LKVEIISTEIIKPSSPTPSDHQQHKLSLLDQFAPSSYTPLLFFYPADQLHGADDHRDRCR 65

Query: 63  KLKESLAETLTRFYLLAGTLVEAYLVECNDEGVEFSEARVACQLADVMDKPDDVAALHRL 122
           KLKESLAETL+RFYLLAGTLVEAYLVECND+GV F+EARV+ +L++VM+ P DV  LHRL
Sbjct: 66  KLKESLAETLSRFYLLAGTLVEAYLVECNDDGVAFTEARVSRRLSEVMENPHDVVLLHRL 125

Query: 123 LPFDPDAVL-------DRDCILAVRYNAFDCGGVVIALCVTHKIVDGTSATTFTKAWAAA 182
           LPF PDAVL       +R+CILAVRYN F+CGGVVIALCVTHKIVDGTSAT FTKAWAA 
Sbjct: 126 LPFQPDAVLXXXXAVLERECILAVRYNVFECGGVVIALCVTHKIVDGTSATLFTKAWAAT 185

Query: 183 CRGDHELTVVPTFDAAELFPPMEIRGGNKRQSRMDKIVTKRFIFSKSNIAALKQEANSDA 242
           CRGD+E  VVPTF+AAELFPPME  GGNKRQSRM KIVT+RFIF+KSNIAALK++ANSD 
Sbjct: 186 CRGDNEYPVVPTFNAAELFPPMENHGGNKRQSRMQKIVTRRFIFNKSNIAALKKQANSDE 245

Query: 243 -FLNQRPPSRVETVSAFIWKRFMALARSKPTTTAAKRFAILHAVNIRNRLSPPLPPHSFG 302
            FLNQRPPSRVE+VS F WKRF+ALA  KP T A KRFA++ AVN+RNR +PPLPPHSFG
Sbjct: 246 LFLNQRPPSRVESVSGFFWKRFIALAHQKPPTKA-KRFAVIQAVNLRNRTNPPLPPHSFG 305

Query: 303 NLWWFAVAEAPIEEEKDYPFLVSKIRNAITEIDGEYVTALQDAEKAMRAKMKMGQMVYSG 362
           N+WWFA A+API+EE ++P LV+KIRNAI EIDG Y+  LQ AEK MR KMKMGQMVYSG
Sbjct: 306 NIWWFATADAPIDEEPEFPSLVAKIRNAINEIDGHYIKTLQHAEKTMREKMKMGQMVYSG 365

Query: 363 EVELYSFTSWCSFPIYESDFGWGRPAWVCSPGRPYKNVVLLVNTGDGEGIEAWVNLEEKD 422
           EVEL SFT WC+FP+YESDFGWG+P WVCSPGRPYKNVVL VNT DGEGIEAWVNLEE D
Sbjct: 366 EVELISFTGWCNFPLYESDFGWGKPMWVCSPGRPYKNVVLFVNTSDGEGIEAWVNLEEND 425

Query: 423 MPVFESDPELLAFTSSS 430
           M VFE+DP+LL+FTSSS
Sbjct: 426 MKVFETDPQLLSFTSSS 441

BLAST of MC03g0977 vs. ExPASy TrEMBL
Match: A0A6J1CAK2 (vinorine synthase-like OS=Momordica charantia OX=3673 GN=LOC111008950 PE=3 SV=1)

HSP 1 Score: 871 bits (2250), Expect = 0.0
Identity = 431/431 (100.00%), Postives = 431/431 (100.00%), Query Frame = 0

Query: 1   MELKVEIISTESIKPSSPTPPEQRELKLSLLDQVAPGSYTPLLFFYPAGQLDRGDHGGRC 60
           MELKVEIISTESIKPSSPTPPEQRELKLSLLDQVAPGSYTPLLFFYPAGQLDRGDHGGRC
Sbjct: 1   MELKVEIISTESIKPSSPTPPEQRELKLSLLDQVAPGSYTPLLFFYPAGQLDRGDHGGRC 60

Query: 61  RKLKESLAETLTRFYLLAGTLVEAYLVECNDEGVEFSEARVACQLADVMDKPDDVAALHR 120
           RKLKESLAETLTRFYLLAGTLVEAYLVECNDEGVEFSEARVACQLADVMDKPDDVAALHR
Sbjct: 61  RKLKESLAETLTRFYLLAGTLVEAYLVECNDEGVEFSEARVACQLADVMDKPDDVAALHR 120

Query: 121 LLPFDPDAVLDRDCILAVRYNAFDCGGVVIALCVTHKIVDGTSATTFTKAWAAACRGDHE 180
           LLPFDPDAVLDRDCILAVRYNAFDCGGVVIALCVTHKIVDGTSATTFTKAWAAACRGDHE
Sbjct: 121 LLPFDPDAVLDRDCILAVRYNAFDCGGVVIALCVTHKIVDGTSATTFTKAWAAACRGDHE 180

Query: 181 LTVVPTFDAAELFPPMEIRGGNKRQSRMDKIVTKRFIFSKSNIAALKQEANSDAFLNQRP 240
           LTVVPTFDAAELFPPMEIRGGNKRQSRMDKIVTKRFIFSKSNIAALKQEANSDAFLNQRP
Sbjct: 181 LTVVPTFDAAELFPPMEIRGGNKRQSRMDKIVTKRFIFSKSNIAALKQEANSDAFLNQRP 240

Query: 241 PSRVETVSAFIWKRFMALARSKPTTTAAKRFAILHAVNIRNRLSPPLPPHSFGNLWWFAV 300
           PSRVETVSAFIWKRFMALARSKPTTTAAKRFAILHAVNIRNRLSPPLPPHSFGNLWWFAV
Sbjct: 241 PSRVETVSAFIWKRFMALARSKPTTTAAKRFAILHAVNIRNRLSPPLPPHSFGNLWWFAV 300

Query: 301 AEAPIEEEKDYPFLVSKIRNAITEIDGEYVTALQDAEKAMRAKMKMGQMVYSGEVELYSF 360
           AEAPIEEEKDYPFLVSKIRNAITEIDGEYVTALQDAEKAMRAKMKMGQMVYSGEVELYSF
Sbjct: 301 AEAPIEEEKDYPFLVSKIRNAITEIDGEYVTALQDAEKAMRAKMKMGQMVYSGEVELYSF 360

Query: 361 TSWCSFPIYESDFGWGRPAWVCSPGRPYKNVVLLVNTGDGEGIEAWVNLEEKDMPVFESD 420
           TSWCSFPIYESDFGWGRPAWVCSPGRPYKNVVLLVNTGDGEGIEAWVNLEEKDMPVFESD
Sbjct: 361 TSWCSFPIYESDFGWGRPAWVCSPGRPYKNVVLLVNTGDGEGIEAWVNLEEKDMPVFESD 420

Query: 421 PELLAFTSSSS 431
           PELLAFTSSSS
Sbjct: 421 PELLAFTSSSS 431

BLAST of MC03g0977 vs. ExPASy TrEMBL
Match: A0A6J1KKZ8 (vinorine synthase-like OS=Cucurbita maxima OX=3661 GN=LOC111495142 PE=3 SV=1)

HSP 1 Score: 696 bits (1796), Expect = 3.20e-250
Identity = 339/429 (79.02%), Postives = 381/429 (88.81%), Query Frame = 0

Query: 3   LKVEIISTESIKPSSPTPPEQRELKLSLLDQVAPGSYTPLLFFYPAGQLDRGD-HGGRCR 62
           L VEIISTESIKPSSPTP E RELKLS LDQVAP SYTPLLFFY A QL  GD H  RCR
Sbjct: 6   LNVEIISTESIKPSSPTPSEHRELKLSFLDQVAPSSYTPLLFFYTADQLHGGDDHRERCR 65

Query: 63  KLKESLAETLTRFYLLAGTLVEAYLVECNDEGVEFSEARVACQLADVMDKPDDVAALHRL 122
           KLKESLAETL RFYLLAGTLVEAYLV+CND+GVEFSE RV+ ++++VM+KP DV +L RL
Sbjct: 66  KLKESLAETLNRFYLLAGTLVEAYLVDCNDDGVEFSETRVSARMSEVMEKPHDVVSLLRL 125

Query: 123 LPFDPDAVLDRDCILAVRYNAFDCGGVVIALCVTHKIVDGTSATTFTKAWAAACRGDHEL 182
           LPFD DAVL+R+CILAVRYN F+CGGVV+ALCVTHKIVDGTSAT FTKAWAAACRGD + 
Sbjct: 126 LPFDHDAVLERECILAVRYNVFECGGVVVALCVTHKIVDGTSATMFTKAWAAACRGDDKY 185

Query: 183 TVVPTFDAAELFPPMEIRGGNKRQSRMDKIVTKRFIFSKSNIAALKQEANSDAFLNQRPP 242
            VVPTF+A ELFPPMEIRGGN+RQSRMDKIVT+RFIF+KSNIAALK +ANS+AFLNQRPP
Sbjct: 186 PVVPTFNATELFPPMEIRGGNRRQSRMDKIVTRRFIFNKSNIAALKNQANSEAFLNQRPP 245

Query: 243 SRVETVSAFIWKRFMALARSKPTTTAAKRFAILHAVNIRNRLSPPLPPHSFGNLWWFAVA 302
           SRVETVS F+WKRFMA+A+ KP T A KRFA++ AVN+RNR+ PPLPP+SFGNLWWFA A
Sbjct: 246 SRVETVSGFLWKRFMAIAQQKPPTKA-KRFAVIQAVNLRNRMDPPLPPNSFGNLWWFATA 305

Query: 303 EAPIEEEKDYPFLVSKIRNAITEIDGEYVTALQDAEKAMRAKMKMGQMVYSGEVELYSFT 362
           +API+EE+D+P LV+KIR AI EIDG+Y+  LQDAEK+M+AKMKMGQMVYSGE+ELYSFT
Sbjct: 306 DAPIDEEQDFPSLVAKIREAIKEIDGDYIKTLQDAEKSMKAKMKMGQMVYSGELELYSFT 365

Query: 363 SWCSFPIYESDFGWGRPAWVCSPGRPYKNVVLLVNTGDGEGIEAWVNLEEKDMPVFESDP 422
           SWCSFP+YESDFGWG+PAWVCSPGRPYKNVVL VNT DGEGIEAWVNL E DM  FE+D 
Sbjct: 366 SWCSFPLYESDFGWGKPAWVCSPGRPYKNVVLFVNTSDGEGIEAWVNLTETDMKDFETDS 425

Query: 423 ELLAFTSSS 430
           ELL+F S S
Sbjct: 426 ELLSFASLS 433

BLAST of MC03g0977 vs. ExPASy TrEMBL
Match: A0A6J1E6N5 (vinorine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111431282 PE=3 SV=1)

HSP 1 Score: 694 bits (1792), Expect = 1.30e-249
Identity = 338/429 (78.79%), Postives = 381/429 (88.81%), Query Frame = 0

Query: 3   LKVEIISTESIKPSSPTPPEQRELKLSLLDQVAPGSYTPLLFFYPAGQLDRGD-HGGRCR 62
           L VEIISTESIKPSSPTP E RELKLS LDQVAP SYTPLLFFY A QL  GD H  RCR
Sbjct: 6   LNVEIISTESIKPSSPTPSEHRELKLSFLDQVAPSSYTPLLFFYTADQLHGGDDHRERCR 65

Query: 63  KLKESLAETLTRFYLLAGTLVEAYLVECNDEGVEFSEARVACQLADVMDKPDDVAALHRL 122
           KLKESLAETL RFYLLAGTLVEAYLV+CND+GVEFSEARV+ ++++VM+KP DV +L RL
Sbjct: 66  KLKESLAETLNRFYLLAGTLVEAYLVDCNDDGVEFSEARVSGRMSEVMEKPHDVVSLLRL 125

Query: 123 LPFDPDAVLDRDCILAVRYNAFDCGGVVIALCVTHKIVDGTSATTFTKAWAAACRGDHEL 182
           LPFD DAVL+R+CILAVRYN F+CGGVV+ALCVTHKIVDGTSAT FTKAWAAACRGD + 
Sbjct: 126 LPFDHDAVLERECILAVRYNVFECGGVVVALCVTHKIVDGTSATMFTKAWAAACRGDDKY 185

Query: 183 TVVPTFDAAELFPPMEIRGGNKRQSRMDKIVTKRFIFSKSNIAALKQEANSDAFLNQRPP 242
            VVPTFDA ELFPPMEIRGGN+RQSRM+KIVT+RFIF+KSNIAALK +ANS+AFLNQRPP
Sbjct: 186 PVVPTFDATELFPPMEIRGGNRRQSRMEKIVTRRFIFNKSNIAALKHQANSEAFLNQRPP 245

Query: 243 SRVETVSAFIWKRFMALARSKPTTTAAKRFAILHAVNIRNRLSPPLPPHSFGNLWWFAVA 302
           SRVETVS F+WKRFMA+A+ KP T A KRFA++ AVN+RNR+ PPLPP+SFGNLWWFA A
Sbjct: 246 SRVETVSGFLWKRFMAIAQQKPPTKA-KRFAVIQAVNLRNRMDPPLPPNSFGNLWWFATA 305

Query: 303 EAPIEEEKDYPFLVSKIRNAITEIDGEYVTALQDAEKAMRAKMKMGQMVYSGEVELYSFT 362
           +API+EE+D+P LV+KIR AI EIDG+Y+  +QD EK+M+AKMKMGQMVYSGE+ELYSFT
Sbjct: 306 DAPIDEEQDFPSLVAKIREAIKEIDGDYIKTIQDTEKSMKAKMKMGQMVYSGELELYSFT 365

Query: 363 SWCSFPIYESDFGWGRPAWVCSPGRPYKNVVLLVNTGDGEGIEAWVNLEEKDMPVFESDP 422
           SWCSFP+YESDFGWG+PAWVCSPGRPYKNVVL VNT DGEGIEAWVNL E DM  FE+D 
Sbjct: 366 SWCSFPLYESDFGWGKPAWVCSPGRPYKNVVLFVNTSDGEGIEAWVNLTETDMKDFETDA 425

Query: 423 ELLAFTSSS 430
           ELL+F S S
Sbjct: 426 ELLSFASLS 433

BLAST of MC03g0977 vs. ExPASy TrEMBL
Match: A0A5A7UJX0 (Vinorine synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G004430 PE=3 SV=1)

HSP 1 Score: 640 bits (1650), Expect = 4.80e-228
Identity = 309/429 (72.03%), Postives = 359/429 (83.68%), Query Frame = 0

Query: 3   LKVEIISTESIKPSSPTPPEQRELKLSLLDQVAPGSYTPLLFFYPAGQLDRGDHGGRCRK 62
           LK+EI STE IKPSSPTP   +  KLS LDQ APGSYTPLLFFYP       DH  RCRK
Sbjct: 7   LKIEITSTEIIKPSSPTPSTHQHHKLSFLDQFAPGSYTPLLFFYPG----HADHRDRCRK 66

Query: 63  LKESLAETLTRFYLLAGTLVEAYLVECNDEGVEFSEARVACQLADVMDKPDDVAALHRLL 122
           LKESLAETL+RFYLLAGTLVEAYLVECNDEGV FSE RV+ +L++VM+ P+D+ +LHRLL
Sbjct: 67  LKESLAETLSRFYLLAGTLVEAYLVECNDEGVAFSEVRVSGRLSEVMENPNDLVSLHRLL 126

Query: 123 PFDPDAVLDRDCILAVRYNAFDCGGVVIALCVTHKIVDGTSATTFTKAWAAACRGDHELT 182
           PF PDAVL+R+CIL V+YN F+CGG VI LCVTHKIVDGTSAT FTKAWA+ CRGD + +
Sbjct: 127 PFHPDAVLERECILGVQYNVFECGGAVIGLCVTHKIVDGTSATMFTKAWASTCRGDSQCS 186

Query: 183 VVPTFDAAELFPPMEIRGGNKRQSRMDKIVTKRFIFSKSNIAALKQEANSDA-FLNQRPP 242
           +VPTFDA ELFP MEIRGGN+RQSRM K+VT+RFIF+KSNIAALK++ANS A FLNQRPP
Sbjct: 187 IVPTFDATELFPAMEIRGGNRRQSRMQKVVTRRFIFNKSNIAALKKQANSAALFLNQRPP 246

Query: 243 SRVETVSAFIWKRFMALARSKPTTTAAKRFAILHAVNIRNRLSPPLPPHSFGNLWWFAVA 302
           SRVE+VS F+W RF+AL+  KP T A KRF  +  VN+R+R++PPLPPHSFGN+ W A A
Sbjct: 247 SRVESVSGFLWNRFIALSHQKPPTKA-KRFVAIQTVNLRSRMNPPLPPHSFGNICWSATA 306

Query: 303 EAPIEEEKDYPFLVSKIRNAITEIDGEYVTALQDAEKAMRAKMKMGQMVYSGEVELYSFT 362
           E PI+E++ +PFLV KIR AI EID EY   LQD EK+MRAKMKMG+ VYSGEVE+  FT
Sbjct: 307 EVPIDEKQHFPFLVGKIREAIKEIDDEYTKTLQDTEKSMRAKMKMGERVYSGEVEMVCFT 366

Query: 363 SWCSFPIYESDFGWGRPAWVCSPGRPYKNVVLLVNTGDGEGIEAWVNLEEKDMPVFESDP 422
           SWC+FP+YE+DFGWG+P WVC+PGRPYKNVVL VNT DGEGIEAWVNLEE DM +FE+D 
Sbjct: 367 SWCNFPVYETDFGWGKPTWVCTPGRPYKNVVLFVNTSDGEGIEAWVNLEENDMALFENDS 426

Query: 423 ELLAFTSSS 430
           ELL+FTSSS
Sbjct: 427 ELLSFTSSS 430

BLAST of MC03g0977 vs. ExPASy TrEMBL
Match: E5GBW2 (Anthranilate N-benzoyltransferase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 640 bits (1650), Expect = 4.80e-228
Identity = 309/429 (72.03%), Postives = 359/429 (83.68%), Query Frame = 0

Query: 3   LKVEIISTESIKPSSPTPPEQRELKLSLLDQVAPGSYTPLLFFYPAGQLDRGDHGGRCRK 62
           LK+EI STE IKPSSPTP   +  KLS LDQ APGSYTPLLFFYP       DH  RCRK
Sbjct: 7   LKIEITSTEIIKPSSPTPSTHQHHKLSFLDQFAPGSYTPLLFFYPG----HADHRDRCRK 66

Query: 63  LKESLAETLTRFYLLAGTLVEAYLVECNDEGVEFSEARVACQLADVMDKPDDVAALHRLL 122
           LKESLAETL+RFYLLAGTLVEAYLVECNDEGV FSE RV+ +L++VM+ P+D+ +LHRLL
Sbjct: 67  LKESLAETLSRFYLLAGTLVEAYLVECNDEGVAFSEVRVSGRLSEVMENPNDLVSLHRLL 126

Query: 123 PFDPDAVLDRDCILAVRYNAFDCGGVVIALCVTHKIVDGTSATTFTKAWAAACRGDHELT 182
           PF PDAVL+R+CIL V+YN F+CGG VI LCVTHKIVDGTSAT FTKAWA+ CRGD + +
Sbjct: 127 PFHPDAVLERECILGVQYNVFECGGAVIGLCVTHKIVDGTSATMFTKAWASTCRGDSQCS 186

Query: 183 VVPTFDAAELFPPMEIRGGNKRQSRMDKIVTKRFIFSKSNIAALKQEANSDA-FLNQRPP 242
           +VPTFDA ELFP MEIRGGN+RQSRM K+VT+RFIF+KSNIAALK++ANS A FLNQRPP
Sbjct: 187 IVPTFDATELFPAMEIRGGNRRQSRMQKVVTRRFIFNKSNIAALKKQANSAALFLNQRPP 246

Query: 243 SRVETVSAFIWKRFMALARSKPTTTAAKRFAILHAVNIRNRLSPPLPPHSFGNLWWFAVA 302
           SRVE+VS F+W RF+AL+  KP T A KRF  +  VN+R+R++PPLPPHSFGN+ W A A
Sbjct: 247 SRVESVSGFLWNRFIALSHQKPPTKA-KRFVAIQTVNLRSRMNPPLPPHSFGNICWSATA 306

Query: 303 EAPIEEEKDYPFLVSKIRNAITEIDGEYVTALQDAEKAMRAKMKMGQMVYSGEVELYSFT 362
           E PI+E++ +PFLV KIR AI EID EY   LQD EK+MRAKMKMG+ VYSGEVE+  FT
Sbjct: 307 EVPIDEKQHFPFLVGKIREAIKEIDDEYTKTLQDTEKSMRAKMKMGERVYSGEVEMVCFT 366

Query: 363 SWCSFPIYESDFGWGRPAWVCSPGRPYKNVVLLVNTGDGEGIEAWVNLEEKDMPVFESDP 422
           SWC+FP+YE+DFGWG+P WVC+PGRPYKNVVL VNT DGEGIEAWVNLEE DM +FE+D 
Sbjct: 367 SWCNFPVYETDFGWGKPTWVCTPGRPYKNVVLFVNTSDGEGIEAWVNLEENDMALFENDS 426

Query: 423 ELLAFTSSS 430
           ELL+FTSSS
Sbjct: 427 ELLSFTSSS 430

BLAST of MC03g0977 vs. TAIR 10
Match: AT3G26040.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 288.5 bits (737), Expect = 8.9e-78
Identity = 169/438 (38.58%), Postives = 257/438 (58.68%), Query Frame = 0

Query: 3   LKVEIISTESIKPSSPTPPEQRELKLSLLDQVAPGSYTPLLFFYPAGQLDRGDHGGRCRK 62
           ++V+++S + IKPSSPTP   ++ KLSLL+Q+ P  + P++FFY A    +     + + 
Sbjct: 1   MRVDVVSRDIIKPSSPTPNHLKKFKLSLLEQLGPTIFGPMVFFYSANNSIKPTE--QLQM 60

Query: 63  LKESLAETLTRFYLLAGTLVEAYLVECNDEGVEFSEARVACQLADVMDKPDDVAALHRLL 122
           LK+SL+ETLT FY LAG L     ++CND G +F EARV   L++++ +P    +L +L+
Sbjct: 61  LKKSLSETLTHFYPLAGRLKGNISIDCNDSGADFLEARVNSPLSNLLLEPSS-DSLQQLI 120

Query: 123 PFDPDAVLDRDCILAVRYNAFDCGGVVIALCVTHKIVDGTSATTFTKAWAA-ACRGDHEL 182
           P   D++  R  +L  + + F+CG + I +C++HK+ D TS   F K+WAA + RG  + 
Sbjct: 121 PTSVDSIETRTRLLLAQASFFECGSMSIGVCISHKLADATSIGLFMKSWAAISSRGSIKT 180

Query: 183 TVVPTFDAAELFPPMEIRGGN-----KRQSRMDKIVTKRFIFSKSNIAALKQEANSDAFL 242
              P FD  ++FPP      +     + +  M++ ++KRFIF  S+I AL+ +A+S    
Sbjct: 181 IGAPVFDTVKIFPPGNFSETSPAPVVEPEIMMNQTLSKRFIFDSSSIQALQAKASS---F 240

Query: 243 NQRPPSRVETVSAFIWKRFMALARSKPTTTAAKRFAILHAVNIRNRLSPPLPPHSFGNL- 302
               P+RVE VSA IWK  M   R+   T  +K   + ++V++R+R+SPP   +S GNL 
Sbjct: 241 EVNQPTRVEAVSALIWKSAMKATRTVSGT--SKPSILANSVSLRSRVSPPFTKNSIGNLV 300

Query: 303 -WWFAVAEAPIEEEKDYPFLVSKIRNAITEIDGEYVTALQDAEKAMRA----KMKMGQMV 362
            ++ A AE  I + K    LVSKIR A       ++  L     A       + + G M+
Sbjct: 301 SYFAAKAEEGINQTK-LQTLVSKIRKAKQRFRDIHIPKLVGNPNATEIICSYQKEAGDMI 360

Query: 363 YSGEVELYSFTSWCSFPIYESDFGWGRPAWVCSPGRPYKNVVLLVNTGDGEGIEAWVNLE 422
            SG+ + Y F+S C F +YE+DFGWG+P WV  P    KN+V L++T +  GIEAWVNL 
Sbjct: 361 ASGDFDFYIFSSACRFGLYETDFGWGKPVWVGFPSVRQKNIVTLLDTKEAGGIEAWVNLN 420

Query: 423 EKDMPVFESDPELLAFTS 429
           E++M +FE D ELL F S
Sbjct: 421 EQEMNLFEQDRELLQFAS 429

BLAST of MC03g0977 vs. TAIR 10
Match: AT1G24430.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 283.9 bits (725), Expect = 2.2e-76
Identity = 162/443 (36.57%), Postives = 250/443 (56.43%), Query Frame = 0

Query: 1   MELKVEIISTESIKPSSPT---PPEQRELKLSLLDQVAPGSYTPLLFFY----PAGQLDR 60
           M L++ + S E +KPS      PP      LS LDQ+AP  + P LFFY         +R
Sbjct: 1   MGLEITVTSQELVKPSPRNLNHPPCHHH--LSFLDQLAPPIFMPFLFFYHNKTNLSDKER 60

Query: 61  GDHGGRCRKLKESLAETLTRFYLLAGTLVEA-YLVECNDEGVEFSEARVACQLADVMDKP 120
            DH      +K SL+E L  +Y LAG +  +  +V CND GV F EA+  C ++ +++ P
Sbjct: 61  SDH------IKSSLSEILNLYYPLAGRIKNSGDVVVCNDVGVSFVEAKADCNMSQILENP 120

Query: 121 DDVAALHRLLPFDPDAVLDRDCILAVRYNAFDCGGVVIALCVTHKIVDGTSATTFTKAWA 180
           +    L++L PF+   V   D  L V+   F+CGG+ + + ++HK+ D  S   F  +WA
Sbjct: 121 NP-NELNKLHPFEFHEV--SDVPLTVQLTFFECGGLALGIGLSHKLCDALSGLIFVNSWA 180

Query: 181 AACRGDHELTVVPTFDAAELFPPMEIRGGNKRQS-RMDKIVTKRFIFSKSNIAALKQEAN 240
           A  RG  +  + P+FD A++FPP +I   N       + IVT+RF+F +S++ +L++  +
Sbjct: 181 AFARGQTDEIITPSFDLAKMFPPCDIENLNMATGITKENIVTRRFVFLRSSVESLRERFS 240

Query: 241 SDAFLNQRPPSRVETVSAFIWKRFMALARSKPTTTAAKRFAILHAVNIRNRLSPPLPPHS 300
            +  +     +RVE +S FIW RFMA       T   K + ++H VN+R +  P +P + 
Sbjct: 241 GNKKIR---ATRVEVLSVFIWSRFMASTNHDDKT--GKIYTLIHPVNLRRQADPDIPDNM 300

Query: 301 FGNLWWFAVAEAPI---EEEKDYPFLVSKIRNAITEIDGEYVTALQDAEKA-MRAKMKMG 360
           FGN+  F+V    +   E +++   LV ++R  I +ID  YV  LQ+  +  +    K  
Sbjct: 301 FGNIMRFSVTVPMMIINENDEEKASLVDQMREEIRKIDAVYVKKLQEDNRGHLEFLNKQA 360

Query: 361 QMVYSGEVELYSFTSWCSFPIYESDFGWGRPAWVCSPGRPYKNVVLLVNTGDGEGIEAWV 420
               +GE+  +SFTS C FP+YE+DFGWG+P WV S    YKN+V  ++T +G+GIEAW+
Sbjct: 361 SGFVNGEIVSFSFTSLCKFPVYEADFGWGKPLWVASARMSYKNLVAFIDTKEGDGIEAWI 420

Query: 421 NLEEKDMPVFESDPELLAFTSSS 431
           NL++ DM  FE+D ELL + SS+
Sbjct: 421 NLDQNDMSRFEADEELLRYVSSN 427

BLAST of MC03g0977 vs. TAIR 10
Match: AT4G15390.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 252.3 bits (643), Expect = 7.0e-67
Identity = 161/446 (36.10%), Postives = 235/446 (52.69%), Query Frame = 0

Query: 1   MELKVEIISTESIKPSSPTPPEQRELKLSLLDQVAPGSYTPLLFFYPAGQLDRGDHGGRC 60
           M +KVE IS E IKPSSPTP   + L+LS+ D + P  YT    FY    L   +H    
Sbjct: 4   MTMKVETISKEIIKPSSPTPNNLQTLQLSIYDHILPPVYTVAFLFYTKNDLISQEH--TS 63

Query: 61  RKLKESLAETLTRFYLLAGTLVEAYLVECNDEGVEFSEARV-ACQLADVMDKPDDVAALH 120
            KLK SL+ETLT+FY LAG  +    V+C DEG  F +ARV  C L + +  P D  AL 
Sbjct: 64  HKLKTSLSETLTKFYPLAGR-ITGVTVDCTDEGAIFVDARVNNCPLTEFLKCP-DFDALQ 123

Query: 121 RLLPFD----PDAVLDRDCILAVRYNAFDCGGVVIALCVTHKIVDGTSATTFTKAWAAAC 180
           +LLP D    P        +L V+   F CGG+ I +C+THKI D  S +TF ++WAA  
Sbjct: 124 QLLPLDVVDNPYVAAATWPLLLVKATYFGCGGMAIGICITHKIADAASISTFIRSWAATA 183

Query: 181 RGDHELTVV--PTFDAAELFPPME--IRGGNKRQSRMDKIVTKRFIFSKSNIAALKQEAN 240
           RG+++   +  P F  A  +PP     +     Q+     +TKRF+F  S +  L+ +A 
Sbjct: 184 RGENDAAAMESPVFAGANFYPPANEAFKLPADEQAGKRSSITKRFVFEASKVEDLRTKAA 243

Query: 241 SDAFLNQRPPSRVETVSAFIWKRFMALARSKPTTTAAKRFAILHAVNIRNRLSPPLPPHS 300
           S+  ++Q  P+RVE+V+A IWK F+A ++    TT      ++   N+R+++   L   S
Sbjct: 244 SEETVDQ--PTRVESVTALIWKCFVASSK----TTTCDHKVLVQLANLRSKIPSLLQESS 303

Query: 301 FGNLWWFAV-------AEAPIEEE-KDYPFLVSKIRNAITEIDGEYVTALQDAEKAMRAK 360
            GNL + +V        E  IEE  +D      ++   I +  G   ++     K     
Sbjct: 304 IGNLMFSSVVLSIGRGGEVKIEEAVRDLRKKKEELGTVILDEGGSSDSSSMIGSKLANLM 363

Query: 361 MKMGQMVYSGEVELYSFTSWCSFPIYESDFGWGRPAWVCSPGRP-YKNVVLLVNTGDGEG 420
           +     +     E Y+ +SWC  P+YE+ FGW  P WV     P   N+ +L+++ DG+G
Sbjct: 364 LTNYSRLSYETHEPYTVSSWCKLPLYEASFGWDSPVWVVGNVSPVLGNLAMLIDSKDGQG 423

Query: 421 IEAWVNLEEKDMPVFESDPELLAFTS 429
           IEA+V L E++M  FE +PELLAF +
Sbjct: 424 IEAFVTLPEENMSSFEQNPELLAFAT 439

BLAST of MC03g0977 vs. TAIR 10
Match: AT3G30280.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 249.2 bits (635), Expect = 6.0e-66
Identity = 164/443 (37.02%), Postives = 239/443 (53.95%), Query Frame = 0

Query: 3   LKVEIISTESIKPSSPTPPEQRELKLSLLDQVAPGSYTPLLFFYPAGQLDRGDHGGRCRK 62
           +KVE I+ E IKPSS TP + + L+LS+ D + P  YT    FY    L   +      K
Sbjct: 4   MKVETIAKEIIKPSSTTPNDLQTLQLSIYDHILPPVYTVAFLFYTKDDLISPEQSS--HK 63

Query: 63  LKESLAETLTRFYLLAGTLVEAYLVECNDEGVEFSEARV-ACQLADVMDKPDDVAALHRL 122
           LK SLAETLT+FY LAG  ++   ++CNDEG  F +ARV    L+D +  P D   L +L
Sbjct: 64  LKTSLAETLTKFYPLAGR-IKGVTIDCNDEGAVFVDARVNNYPLSDFLRSP-DFKTLQQL 123

Query: 123 LPF----DPDAVLDRDCILAVRYNAFDCGGVVIALCVTHKIVDGTSATTFTKAWAAACRG 182
           LP     DP        +L V+   F CGG+ I LC+THKI D TS +TF ++WAA  RG
Sbjct: 124 LPLDVIDDPYEAATTWPLLLVKATYFPCGGMAIGLCITHKIADATSISTFIQSWAAMARG 183

Query: 183 D-HELTVVPTFDAAELFPPMEIRGGNKRQSRMDKI--VTKRFIFSKSNIAALKQEANSDA 242
           +  ++   P F AA  +PP             +KI  +TKRF+FS S +  L+ +A S+ 
Sbjct: 184 EAGDIVADPVFAAANFYPPANELFKFPVDENANKISQITKRFVFSASKLEELRTKAASED 243

Query: 243 FLNQRPPSRVETVSAFIWKRFMALARSKPTTTAAKRFAILHAVNIRNRLSPPLPPHSFGN 302
           F+ +  P RVE+V+A +WK F+A A S   T A     ++   N+R ++   L     GN
Sbjct: 244 FVGR--PKRVESVTALLWKAFVAAASS--NTNACDMKVLIQPSNLRPKIPSLLAESLIGN 303

Query: 303 LWWFAVA-------EAPIEEE-KDYPFLVSKIRNAITEIDGEYVTALQDAEKAMRAKMKM 362
           + + +V        E  IE+  +D       ++N I E +G   +A+  ++ A       
Sbjct: 304 IMFSSVVLGISQQEEIKIEKAVRDLRKQGDDLQNVIKE-EGGSSSAMIGSKLANLMLTNY 363

Query: 363 GQMVYSGEVELYSFTSWCSFPIYESDFGWGRPAWVCSPGRP-YKNVVLLVNTGDGEGIEA 422
            ++ Y    E Y+ +SWC  P+YE+ FGWG P W+     P  +NV +L+++ DG+GIEA
Sbjct: 364 SKLSYETH-EPYTVSSWCKLPLYEASFGWGSPVWIAGNVAPMLENVTMLIDSKDGQGIEA 423

Query: 423 WVNLEEKDMPVFESDPELLAFTS 429
           +V L E++M   E + ELLAF S
Sbjct: 424 FVTLPEENMLSLEQNTELLAFAS 436

BLAST of MC03g0977 vs. TAIR 10
Match: AT1G24420.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 247.7 bits (631), Expect = 1.7e-65
Identity = 165/444 (37.16%), Postives = 246/444 (55.41%), Query Frame = 0

Query: 1   MELKVEIISTESIKPSSPTPPEQRELKLSLLDQVAPGSYTPLLFFYPAG--QLDRGDHGG 60
           ME  VEI+S E +KPSSPTP ++R L LSLLD ++   YT  L FY A    L       
Sbjct: 1   MEKNVEILSREIVKPSSPTPDDKRILNLSLLDILSSPMYTGALLFYAADPQNLLGFSTEE 60

Query: 61  RCRKLKESLAETLTRFYLLAGTLVEAYLVECNDEGVEFSEARVACQLADVMDKPDDVAAL 120
              KLK+SL++TL  FY LAG ++ ++ VECNDEG  F EARV   L++ +  P    +L
Sbjct: 61  TSLKLKKSLSKTLPIFYPLAGRIIGSF-VECNDEGAVFIEARVDHLLSEFLKCPVP-ESL 120

Query: 121 HRLLPFDPDAVLDRDC----ILAVRYNAFDCGGVVIALCVTHKIVDGTSATTFTKAWAAA 180
             L+P +  +   R+     +L ++ N F CGG+VI +CV+HKI D TS   F + WA +
Sbjct: 121 ELLIPVEAKS---REAVTWPVLLIQANFFSCGGLVITICVSHKITDATSLAMFIRGWAES 180

Query: 181 CRGDHELTVVPTFDAAELFPPMEIRGGNKRQSRMDKI-----VTKRFIFSKSNIAALKQE 240
            RG   +T++P+F A+E+FP       +K   R +++     VTKRF+F  S I  L+ +
Sbjct: 181 SRG-LGITLIPSFTASEVFPKPLDELPSKPMDRKEEVEEMSCVTKRFVFDASKIKKLRAK 240

Query: 241 ANSDAFLNQRPPSRVETVSAFIWKRFMALARSKPTTTAAKRFAILHAVNIRNRLSPPLPP 300
           A+ +   N   P+RVE V+A  W+    ++R    T       IL  VN+R ++   L  
Sbjct: 241 ASRNLVKN---PTRVEAVTALFWRCVTKVSRLSSLTPRTSVLQIL--VNLRGKVD-SLCE 300

Query: 301 HSFGNLWWFAV---AEAPIEEEKDYPFLVSKIRNAITEIDGEYVTALQDAEKAMRAKMKM 360
           ++ GN+    +    EA IE  +D   +V +IR A            + + +      ++
Sbjct: 301 NTIGNMLSLMILKNEEAAIERIQD---VVDEIRRAKEIFSLNCKEMSKSSSRIFELLEEI 360

Query: 361 GQMVYSG-EVELYSFTSWCSFPIYESDFGWGRPAWVCSPGRP-YKNVVLLVNTGDGEGIE 420
           G++   G E++L+   SWC   +Y++DFGWG+P WV   G   +KN++LL++T DGEGIE
Sbjct: 361 GKVYGRGNEMDLWMSNSWCKLGLYDADFGWGKPVWVTGRGTSHFKNLMLLIDTKDGEGIE 420

Query: 421 AWVNLEEKDMPVFESDPELLAFTS 429
           AW+ L E+ M +FE D ELL   S
Sbjct: 421 AWITLTEEQMSLFECDQELLESAS 429