MC03g0838 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC03g0838
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	RING-type domain-containing protein
Location	MC03: 15009991 .. 15013721 (-)
RNA-Seq Expression	MC03g0838
Synteny	MC03g0838

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

CTTTTCTCTATTTAAGAGCATTTAACCCATGGGATGTTCTATTGGGCCTGGCCGGTCCATACCACATGGCCCAGTATACGGCGACGTTTTGAAATTGTATCGTTCTCAAATTAACAAACCGAGATTTTGCAATTTGGAATTTACAAACCCTATCCGACCCGGGAACTGGAAGGGAAACTTCGCTCCAATAATTTCTAGGGCTCATCGTCTCCAAATTCTCTGATTTTGTGGAACTCTGGTGTCAATTCAGAGCAACTGAGTTGGAGATAGGGAGAGAAATATGCCTCAGAGACACTCGAAGAATAATAACGATCTTGCTTTCTTCACCTATGATGAGAAGCGGAAGCTCGGGTATGGAACGCAGAAAGAGAGGCTGGGGAAGGACTCGATCAAGCCGTTCGATGTCTGCTGTCTCTGCTTGAAACCCTTCATCGACCCCATGTGCTGCCAGAAAGGCCACACGTTTTGTAAAGAATGCATTCTCGAGTGCCTTCTGGCTCAGAAGAAAGATATTCAGAGGTCTGTTCCTTTTGCCGATTTTAATATTTACATTTGATTGCTTGGTCTCTCTATGCCCCCCAAAATTATTTAAAATTATACGAAGAAGTGGAGGGCGAGCGGTTCAGATCCTTATAAACTATTTGGTATTGGTCTGCCAAAGATGTGCAATTCCAATTTCTTTTTGTAGTCGTATAGCGATCGAATTATAATCGTTTTATGACTTTTTTTTCAGTCTGTTAGTTGTTGTTTTATGTTTGACGTTCTATGTTTGTTCGTGTGCTTTTTTAGGAACTTATAAGATTACTTTGGTCTTTAAAAGGTGAAATTTTGATCCTAAATTGTGGTAAATTGACTGCGATCATTGTAAGTAGTAGTTTATAAAGAAATGAGATGTTACTAATTTACGCTCTTGACGTTATGGTGATGCATGAGAGATTGACCAATGTAAAGTTCTATTTTCCATAAGTCACTTCAATTTTGTGATCCTTCCTGCCACTGTAGTTATAGGCTTAAAAAGGTTTGATGTATTGACAAAAGAAGTCTTCAGTTCGATGAAGATTAAAGACCGTGTTGTTAAATGTGTGGGAATTCACATTAATGGTGGGATTTTAGTTTGATAGTCAGCATTGATCTTATGGTTTTTAGTTGATATAAAAATATTAGACTTGCTAAAATAAGAGGATGGTTGGAGCATTTTGAGAAATGTACATTATAAATGCGAGAGAACCAAATAATGGTGCAGTGAGATGTACTTTCCCCCCTCCATTTTCCTTAAGCTTATTTTTTCAGCCAGCCCATTTGGTGCAATGCAGTTTGGTCTTTCTGTTAATCATGTTTGTCATAAATACCCCTCTGTCAATCCTCAGGAGCATGTTTTTCCTACTGTGCAGGAAGCTTGCTGCATATACTGCTCAGCAGAAGCAAGAGAAGGAAGAAGCAGAAGAGAAACTGATGCAGCAGAAAGCTAGAGAGCTCGATGCATTTGATCAGCAAAACCATGGTGCTGTACCACAATACAATGATCGGAACCACAGCCGGGATAAGAACGGTTTCCATGGGGCAAATAGTGTAAAGGTAACTTCTTATGAAGAAGAAGCACTTCGGAACATGAAGGCATTTTGGCTTCCTTCAGCTACACCAGAAGCTCCCGTCAAAGCAGGAGCTCCTTCTAGCAGTACATTCTGTCCAGAAGGCAACGAGAAACTTAAGCTAAAGTCCCTTTTCCCAATACATTTCACAGAGGACACTAGCGAGCAGAAGAAATCAAAATCTTATGATGTTACATATATATGCCCTAGTTGTAAGGTTACTCTAACGAACACAATGTCTCTGGTGGCCCTTAGCACATGTGGTCATGTGTTCTGTAAGAAGTGTGCTGATAAGTTTATGGCTGTGGATAAAGTTTGTCTCGTCTGCAACAAGGGATGTAAGGGAAGGGATTTGGTGAACTTGGAGAAAGGAGGCACAGGGTTTGCTGCCCATGGGGATTCTCTTGAAGCAACGGACTTCAAGCATTTGGGAAGCGGCTCTGGTTTGGGACTGGTGAGGCCTGCCATGAAGACTTAGTCATCTTGGTAGGAAAGTTAGCCACTTGTGCCCTGTTTGCCTAAGCTGCTTCACGACTGTAATTTGCTTCCATAAATCCTTTATTGCTTTTTTTTTTCCTACTTAACTGGTACGTGGTCTTGTAAAATGGCAATTTTACACCTTGTATTTTCTTAAAAAGAAATAGAAGCTGGATTTTTTTTTAATTACGATTTGCCAAAATTTTTGGTCACTGCAGCCCCTGGCATAATATTGTTTTTTCTGTGAACAACGATATTAACGGTCTTTCGTTGGATGTATCTGGATCCATCAGTGCATAGGATCTGAACATTAGGGGACCTAAAAAAAAGAAAGAAGAAGAAGAAACGAGGAATTGTCGTCGCTGTTGCTACAGAAAGGAAAAAAAAAGTGTTTTCTGCAGGAACCCTTATATTGAAAATCCATCAAATGAGCCTGAGAGGACTAACGATGCAGCTGGTGATTATGCCAAGGCTTGCATTGCTGTTTCTACGGAATGTGGGTTCCTGTGATTGATATTTGGACTAAGATGCAGCAAATTCCTGGCTGGTAAAAGGTTTGTTTGAGGTACTTCTGTTATCTTCACGTGCTAGCTTTGATATTTTTACATAAGCAAATGGTTCAAATGATCTGTTGGCATTGTTGCCGTATTATTTGCAAGTTTTGTTGAACTCTGATTATGTACAGATTTTCCTGGAATTTACAAGATATGGAATTATTTCAGGACATTAAATGTACATTTTTGTCACATCATGTGGCCACAGAATTAGGTGTCATAGATTGCTTAATCTTAAGCTGCCCCTTTTTCCTCTCTTTGGCGGACAAGAACATCTAGGCCTGTCAAAGTTTCCATTTCCTTATGAATTTCACTATGTAAGATGTTAAGTATCTTCGGAAAAGCTTTAAAACGTAGCTATTATTCAGCATTATAAAACAATCTAAACATTCGAAGCTTTAACATGGTATCATAATAGGAGATTTTGAGTTCAAATTTGTATAATCTTGTTTCCTTCTTGTTAGGTCTTCTCCAATTCCCAAACCCACAAGTTAAAATATCTATAAACTGTAACCTATTAGTTTAAGCTTTTGGGTTCTAGTTTAATAACTCTCGGTACAACAAACACAGCATCCCACCCCCACCCTCACTCCCTTCTGCCATGGCAAACCCCTGTGCATATATTTACGAGTTACTCGTCTTGTCCTTGCTCTTGACATGGGAATCTGTAATATTTGCATTAGAGAAATCTGTCCCAACAGGGTTGTTTTGGAGGAAGTCGTCCTGAAGCTGAGAGAAGAAGGATCTGAGTCCAGAAACGCTGCCTGCTGATCTCCCACTCAATGATCCTCTCAAAGCTTTTGAAAAATTGTGATACCAACTTCACTGTAGTCAGTCAGATTCAATGACATCTCAAATTTAGATCAGTTGGCTCAAACTGAGCATTTTCTTAAAGAGTGGCTTAATTTCTTATCACAGACCTATAAGATTCAAGAATGAAATTCATCTACAAATTATTTCCATTGTTTAGAAGAATAATTATTACTAATAGGAGTTTTATTTCTCTTTCTAAATGATTTATGCTGCTTTATGAGATTGTGAGCATGCGGGTCTATCATTAGTTAAATAAATGAACTCCTCAATTAGTTATATCATGATACAAGTATAAATT

mRNA sequence

CTTTTCTCTATTTAAGAGCATTTAACCCATGGGATGTTCTATTGGGCCTGGCCGGTCCATACCACATGGCCCAGTATACGGCGACGTTTTGAAATTGTATCGTTCTCAAATTAACAAACCGAGATTTTGCAATTTGGAATTTACAAACCCTATCCGACCCGGGAACTGGAAGGGAAACTTCGCTCCAATAATTTCTAGGGCTCATCGTCTCCAAATTCTCTGATTTTGTGGAACTCTGGTGTCAATTCAGAGCAACTGAGTTGGAGATAGGGAGAGAAATATGCCTCAGAGACACTCGAAGAATAATAACGATCTTGCTTTCTTCACCTATGATGAGAAGCGGAAGCTCGGGTATGGAACGCAGAAAGAGAGGCTGGGGAAGGACTCGATCAAGCCGTTCGATGTCTGCTGTCTCTGCTTGAAACCCTTCATCGACCCCATGTGCTGCCAGAAAGGCCACACGTTTTGTAAAGAATGCATTCTCGAGTGCCTTCTGGCTCAGAAGAAAGATATTCAGAGGAAGCTTGCTGCATATACTGCTCAGCAGAAGCAAGAGAAGGAAGAAGCAGAAGAGAAACTGATGCAGCAGAAAGCTAGAGAGCTCGATGCATTTGATCAGCAAAACCATGGTGCTGTACCACAATACAATGATCGGAACCACAGCCGGGATAAGAACGGTTTCCATGGGGCAAATAGTGTAAAGGTAACTTCTTATGAAGAAGAAGCACTTCGGAACATGAAGGCATTTTGGCTTCCTTCAGCTACACCAGAAGCTCCCGTCAAAGCAGGAGCTCCTTCTAGCAGTACATTCTGTCCAGAAGGCAACGAGAAACTTAAGCTAAAGTCCCTTTTCCCAATACATTTCACAGAGGACACTAGCGAGCAGAAGAAATCAAAATCTTATGATGTTACATATATATGCCCTAGTTGTAAGGTTACTCTAACGAACACAATGTCTCTGGTGGCCCTTAGCACATGTGGTCATGTGTTCTGTAAGAAGTGTGCTGATAAGTTTATGGCTGTGGATAAAGTTTGTCTCGTCTGCAACAAGGGATGTAAGGGAAGGGATTTGGTGAACTTGGAGAAAGGAGGCACAGGGTTTGCTGCCCATGGGGATTCTCTTGAAGCAACGGACTTCAAGCATTTGGGAAGCGGCTCTGGTTTGGGACTGGTGAGGCCTGCCATGAAGACTTAGTCATCTTGTGCATAGGATCTGAACATTAGGGGACCTAAAAAAAAGAAAGAAGAAGAAGAAACGAGGAATTGTCGTCGCTGTTGCTACAGAAAGGAAAAAAAAAGTGTTTTCTGCAGGAACCCTTATATTGAAAATCCATCAAATGAGCCTGAGAGGACTAACGATGCAGCTGGTGATTATGCCAAGGCTTGCATTGCTGTTTCTACGGAATGTGGGTTCCTGTGATTGATATTTGGACTAAGATGCAGCAAATTCCTGGCTGGTAAAAGGTTTGTTTGAGGTACTTCTGTTATCTTCACGTGCTAGCTTTGATATTTTTACATAAGCAAATGGTTCAAATGATCTGTTGGCATTGTTGCCGTATTATTTGCAAGTTTTGTTGAACTCTGATTATGTACAGATTTTCCTGGAATTTACAAGATATGGAATTATTTCAGGACATTAAATGTACATTTTTGTCACATCATGTGGCCACAGAATTAGGTGTCATAGATTGCTTAATCTTAAGCTGCCCCTTTTTCCTCTCTTTGGCGGACAAGAACATCTAGGCCTGTCAAAGTTTCCATTTCCTTATGAATTTCACTATGTAAGATGTTAAGTATCTTCGGAAAAGCTTTAAAACGTAGCTATTATTCAGCATTATAAAACAATCTAAACATTCGAAGCTTTAACATGGTATCATAATAGGAGATTTTGAGTTCAAATTTGTATAATCTTGTTTCCTTCTTGTTAGGTCTTCTCCAATTCCCAAACCCACAAGTTAAAATATCTATAAACTGTAACCTATTAGTTTAAGCTTTTGGGTTCTAGTTTAATAACTCTCGGTACAACAAACACAGCATCCCACCCCCACCCTCACTCCCTTCTGCCATGGCAAACCCCTGTGCATATATTTACGAGTTACTCGTCTTGTCCTTGCTCTTGACATGGGAATCTGTAATATTTGCATTAGAGAAATCTGTCCCAACAGGGTTGTTTTGGAGGAAGTCGTCCTGAAGCTGAGAGAAGAAGGATCTGAGTCCAGAAACGCTGCCTGCTGATCTCCCACTCAATGATCCTCTCAAAGCTTTTGAAAAATTGTGATACCAACTTCACTGTAGTCAGTCAGATTCAATGACATCTCAAATTTAGATCAGTTGGCTCAAACTGAGCATTTTCTTAAAGAGTGGCTTAATTTCTTATCACAGACCTATAAGATTCAAGAATGAAATTCATCTACAAATTATTTCCATTGTTTAGAAGAATAATTATTACTAATAGGAGTTTTATTTCTCTTTCTAAATGATTTATGCTGCTTTATGAGATTGTGAGCATGCGGGTCTATCATTAGTTAAATAAATGAACTCCTCAATTAGTTATATCATGATACAAGTATAAATT

Coding sequence (CDS)

ATGCCTCAGAGACACTCGAAGAATAATAACGATCTTGCTTTCTTCACCTATGATGAGAAGCGGAAGCTCGGGTATGGAACGCAGAAAGAGAGGCTGGGGAAGGACTCGATCAAGCCGTTCGATGTCTGCTGTCTCTGCTTGAAACCCTTCATCGACCCCATGTGCTGCCAGAAAGGCCACACGTTTTGTAAAGAATGCATTCTCGAGTGCCTTCTGGCTCAGAAGAAAGATATTCAGAGGAAGCTTGCTGCATATACTGCTCAGCAGAAGCAAGAGAAGGAAGAAGCAGAAGAGAAACTGATGCAGCAGAAAGCTAGAGAGCTCGATGCATTTGATCAGCAAAACCATGGTGCTGTACCACAATACAATGATCGGAACCACAGCCGGGATAAGAACGGTTTCCATGGGGCAAATAGTGTAAAGGTAACTTCTTATGAAGAAGAAGCACTTCGGAACATGAAGGCATTTTGGCTTCCTTCAGCTACACCAGAAGCTCCCGTCAAAGCAGGAGCTCCTTCTAGCAGTACATTCTGTCCAGAAGGCAACGAGAAACTTAAGCTAAAGTCCCTTTTCCCAATACATTTCACAGAGGACACTAGCGAGCAGAAGAAATCAAAATCTTATGATGTTACATATATATGCCCTAGTTGTAAGGTTACTCTAACGAACACAATGTCTCTGGTGGCCCTTAGCACATGTGGTCATGTGTTCTGTAAGAAGTGTGCTGATAAGTTTATGGCTGTGGATAAAGTTTGTCTCGTCTGCAACAAGGGATGTAAGGGAAGGGATTTGGTGAACTTGGAGAAAGGAGGCACAGGGTTTGCTGCCCATGGGGATTCTCTTGAAGCAACGGACTTCAAGCATTTGGGAAGCGGCTCTGGTTTGGGACTGGTGAGGCCTGCCATGAAGACTTAG

Protein sequence

MPQRHSKNNNDLAFFTYDEKRKLGYGTQKERLGKDSIKPFDVCCLCLKPFIDPMCCQKGHTFCKECILECLLAQKKDIQRKLAAYTAQQKQEKEEAEEKLMQQKARELDAFDQQNHGAVPQYNDRNHSRDKNGFHGANSVKVTSYEEEALRNMKAFWLPSATPEAPVKAGAPSSSTFCPEGNEKLKLKSLFPIHFTEDTSEQKKSKSYDVTYICPSCKVTLTNTMSLVALSTCGHVFCKKCADKFMAVDKVCLVCNKGCKGRDLVNLEKGGTGFAAHGDSLEATDFKHLGSGSGLGLVRPAMKT

Homology

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BLAST of MC03g0838 vs. ExPASy Swiss-Prot
Match: Q9SY88 (E3 ubiquitin-protein ligase CSU1 OS=Arabidopsis thaliana OX=3702 GN=CSU1 PE=1 SV=1)

HSP 1 Score: 481.9 bits (1239), Expect = 5.4e-135
Identity = 233/307 (75.90%), Postives = 267/307 (86.97%), Query Frame = 0


Query: 1   MPQRHSKNNNDLAFFTYDEKRKLGYGTQKERLGKDSIKPFDVCCLCLKPFIDPMCCQKGH 60
           MPQRHSKNNNDLA+FTYDEK+KLGYGTQ+ERLG+DSIKPFD C LCLKPFIDPMCC KGH
Sbjct: 1   MPQRHSKNNNDLAYFTYDEKKKLGYGTQRERLGRDSIKPFDACSLCLKPFIDPMCCHKGH 60

Query: 61  TFCKECILECLLAQKKDIQRKLAAYTAQQKQEKEEAEEKLMQQKARELDAFDQQNHGAVP 120
            FC+ECILEC LAQKKDIQR+LAA+++Q+KQ+K+E EE+LM QKARELD FDQQNH A+P
Sbjct: 61  VFCRECILECFLAQKKDIQRRLAAHSSQKKQDKDEEEERLMLQKARELDEFDQQNHSAMP 120

Query: 121 QYNDRNHSRDKNGFHGANSVKVTSYEEEALRNMKAFWLPSATPEAPVKAGAPSSSTFCPE 180
           + +D+NH+ DKNGFHGANSVK TS+EEEALR MKAFWLPSATP A V+  AP + T CPE
Sbjct: 121 RNSDKNHNEDKNGFHGANSVKTTSFEEEALRTMKAFWLPSATPAASVRVDAPETHTVCPE 180

Query: 181 GNEKLKLKSLFPIHFTEDTSEQKKSK-------SYDVTYICPSCKVTLTNTMSLVALSTC 240
           G EKLKLK+LF I FTED SE++++K       SYD +YICPSCKVTLTNTMSLVALS+C
Sbjct: 181 GKEKLKLKNLFAIRFTEDNSEEEETKTKSASSSSYDKSYICPSCKVTLTNTMSLVALSSC 240

Query: 241 GHVFCKKCADKFMAVDKVCLVCNKGCKGRDLVNLEKGGTGFAAHGDSLEATDFKHLGSGS 300
           GHVFCKKCA+KFM VDKVCLVC+K CK R+LV L+KGGTGFA H D LEA ++KHLGSGS
Sbjct: 241 GHVFCKKCAEKFMPVDKVCLVCDKPCKDRNLVGLKKGGTGFAEHDDHLEAKEYKHLGSGS 300

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BLAST of MC03g0838 vs. ExPASy Swiss-Prot
Match: Q5U3S7 (Nitric oxide synthase-interacting protein OS=Danio rerio OX=7955 GN=nosip PE=2 SV=1)

HSP 1 Score: 144.4 bits (363), Expect = 2.1e-33
Identity = 98/295 (33.22%), Postives = 151/295 (51.19%), Query Frame = 0


Query: 4   RHSKNNNDLAFFTYDEKRK----LGYGTQKERLGKDSIKPFDVCCLCLKPFIDPMCCQKG 63
           RH KN    A +TY EKRK     GYGTQ  RLGKD+IK FD C L L+P  DP+  + G
Sbjct: 3   RHGKNCTAGAVYTYHEKRKDTAASGYGTQSVRLGKDAIKDFDCCSLSLQPCRDPVLTEDG 62

Query: 64  HTFCKECILECLLAQKKDIQRKLAAYTAQQKQEKEEAEEKLMQQKARELDAFDQQNHGAV 123
           + + KE IL+ +L QK +I +K+ AY  Q++  K E + +   ++    + F Q+ +  V
Sbjct: 63  YLYEKEAILQYILHQKTEIAKKMKAYEKQKQALKSEGQLESKSEERERAEKFKQRENNIV 122

Query: 124 PQ-YNDRNHSRDK-----NGFHGANSVKVTSYEEEALRNMKAFWLPSATPEA-PVKAGAP 183
            +  N     + K     NG   ++S   +S E  +   + +FW+PS TPEA P     P
Sbjct: 123 SKPINPFTSGKSKDEGNQNGSTSSSSTDTSSGESSSSSALPSFWIPSLTPEAKPTLLKKP 182

Query: 184 SSSTFCPEGNEKLKLKSLFPIHFT--EDTSEQKKSKSYDVTYICPSCKVTLTNTMSLVAL 243
           S +  CP     LK+  L  + FT  + + ++    +    Y+C   K TL N++    L
Sbjct: 183 SKTVSCPMSGRPLKMSDLITVRFTPLDPSLDRVALLTRQDRYVCAVTKDTLGNSVPCAVL 242

Query: 244 STCGHVFCKKCADKFMAVDKVCLVCNKGCKGRDLVNLEKGGTGFAAHGDSLEATD 286
              G V   +C +K +  D V  +     K +D++ +++GGTGFA  G  L+A +
Sbjct: 243 RPSGVVVTMECVEKLIKKDMVDPITGDKLKEKDIIPIQRGGTGFAGSGVDLKAKE 297

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BLAST of MC03g0838 vs. ExPASy Swiss-Prot
Match: Q6P829 (Nitric oxide synthase-interacting protein OS=Xenopus tropicalis OX=8364 GN=nosip PE=2 SV=1)

HSP 1 Score: 144.4 bits (363), Expect = 2.1e-33
Identity = 95/289 (32.87%), Postives = 147/289 (50.87%), Query Frame = 0


Query: 4   RHSKNNNDLAFFTYDEKRK----LGYGTQKERLGKDSIKPFDVCCLCLKPFIDPMCCQKG 63
           RH KN    A +TY EK+K     GYGTQ  RL KD++K FD CCL L+P  DP+    G
Sbjct: 3   RHGKNCTAGAVYTYYEKKKDTAASGYGTQTVRLSKDAVKDFDCCCLSLQPCKDPVVTSDG 62

Query: 64  HTFCKECILECLLAQKKDIQRKLAAYTAQQKQEKEEAEEKLMQQKARELDAFDQQNHGAV 123
           + + KE ILE +L QKK+I R++ AY  Q+  +K E +E     K  ++ AF  +    V
Sbjct: 63  YIYEKEAILEYILHQKKEIARQMKAYDKQKNAKKAEMDELNKAAKESQMKAFLDKEMTIV 122

Query: 124 PQYNDRNHSRDKNGFHGANSVKVTSYEEEALRNMKAFWLPSATPEAPVK-AGAPSSSTFC 183
            +  +    +  +G   A         EE  + + +FW+PS TPEA       P  + +C
Sbjct: 123 SKPLNPFTRKSDSGADTAEPSGSQQSSEEKGKQLPSFWIPSLTPEAKTSLVKKPDKTVYC 182

Query: 184 PEGNEKLKLKSLFPIHFT--EDTSEQKKSKSYDVTYICPSCKVTLTNTMSLVALSTCGHV 243
           P   + LK+K L P+HFT  ++  ++    +    Y+C   +  L N++    L   G V
Sbjct: 183 PMSGKPLKMKDLIPVHFTAVDEKVDRVGLINRQDRYVCAVTRDMLGNSVPCAVLRPSGAV 242

Query: 244 FCKKCADKFMAVDKVCLVCNKGCKGRDLVNLEKGGTGFAAHGDSLEATD 286
              +C +K +  D +  +       RD++ L++GGTGF+  G  L+A +
Sbjct: 243 VTMECVEKLIKKDMIDPISGDKLHERDIIMLQRGGTGFSGSGVLLQAKE 291

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BLAST of MC03g0838 vs. ExPASy Swiss-Prot
Match: Q6NUH3 (Nitric oxide synthase-interacting protein OS=Xenopus laevis OX=8355 GN=nosip PE=2 SV=1)

HSP 1 Score: 144.1 bits (362), Expect = 2.7e-33
Identity = 95/289 (32.87%), Postives = 146/289 (50.52%), Query Frame = 0


Query: 4   RHSKNNNDLAFFTYDEKRK----LGYGTQKERLGKDSIKPFDVCCLCLKPFIDPMCCQKG 63
           RH KN    A +TY EK+K     GYGTQ  RL KD++K FD CCL L+P  DP+    G
Sbjct: 3   RHGKNCTAGAVYTYYEKKKDTVASGYGTQTVRLSKDAVKDFDCCCLSLQPCKDPVVTSDG 62

Query: 64  HTFCKECILECLLAQKKDIQRKLAAYTAQQKQEKEEAEEKLMQQKARELDAFDQQNHGAV 123
           + + KE ILE +L QKK+I R++ AY  Q+  +K E EE     K  ++ AF  +    V
Sbjct: 63  YIYEKESILEYILHQKKEIARQMKAYEKQKNSKKAEMEELNKAAKESKMKAFLDKEMTIV 122

Query: 124 PQYNDRNHSRDKNGFHGANSVKVTSYEEEALRNMKAFWLPSATPEAPVK-AGAPSSSTFC 183
            +  +    +  +G   A         EE  + + +FW+PS TPEA       P  + +C
Sbjct: 123 SKPLNPFTRKSDSGIDAAEPSCSQQSSEEKNKQLPSFWIPSLTPEAKTSLVKKPDKTVYC 182

Query: 184 PEGNEKLKLKSLFPIHFT--EDTSEQKKSKSYDVTYICPSCKVTLTNTMSLVALSTCGHV 243
           P     LK+K L P++FT  ++  ++    +    Y+C   +  L N++    L   G V
Sbjct: 183 PMSGRPLKMKDLMPVNFTRVDEKVDRVGLINRQDRYVCAVTRDMLGNSVPCAVLRPSGAV 242

Query: 244 FCKKCADKFMAVDKVCLVCNKGCKGRDLVNLEKGGTGFAAHGDSLEATD 286
              +C +K +  D +  +       RD++ L++GGTGF+  G  L+A +
Sbjct: 243 VTMECVEKLIKKDMIDPISGDKLSERDIIMLQRGGTGFSGSGVLLQAKE 291

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BLAST of MC03g0838 vs. ExPASy Swiss-Prot
Match: Q55DU4 (Nitric oxide synthase-interacting protein homolog OS=Dictyostelium discoideum OX=44689 GN=nosip PE=2 SV=1)

HSP 1 Score: 141.0 bits (354), Expect = 2.3e-32
Identity = 104/330 (31.52%), Postives = 153/330 (46.36%), Query Frame = 0


Query: 4   RHSKNN-NDLAFFTYDEKRKLGYGTQKERLGKDSIKPFDVCCLCLKPFIDPMCCQKGHTF 63
           RHSK++ ++   F+  E++ L YGT  ERLGKDSIK FD C LCL   I P+ C KGH +
Sbjct: 3   RHSKHSCSNSGKFSNYERQLLKYGTTTERLGKDSIKNFDSCSLCLNDVIMPVICNKGHLY 62

Query: 64  CKECILECLLAQKKDI---QRKLAAYTAQQKQEKEEAEEKLMQQKARELDA--------- 123
           CKECIL  L+ QKK I   +++   Y  + K E+++ +EK  Q   +E +          
Sbjct: 63  CKECILTSLIDQKKLIKIKEKEWEQYQNKLKYEQDQKQEKQHQDSIKEFEKNVITLEGIG 122

Query: 124 -------------FDQQNHGAVPQYNDRNHSRDKNGFHGANSVKVTSYEEEALRN----- 183
                         +  N+      N+ N++ + N  +   +   T+     + N     
Sbjct: 123 SNDDNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTTTTTTTTSDVNNKDKEK 182

Query: 184 ---------MKAFWLPSATPEAPVKA-GAPSSSTFCP-EGNEKLKLKSLFPIHFTEDTSE 243
                    + ++W+   TP+   KA   P S T CP +G   LK   L  +HFT     
Sbjct: 183 KEIVEKEIKLNSYWV--ITPDNKDKAIEKPRSYTVCPADGKHPLKSAQLINVHFTNVKPS 242

Query: 244 QKKSKSYDVTYICPSCKVTLTNTMSLVALSTCGHVFCKKCADKF---MAVDKVCLVCNKG 289
              S   +  Y CP C   L+N+     L  CGHVFC  C DKF    +    C VC+K 
Sbjct: 243 SSDSNDSNNQYCCPICSKVLSNSTKTRLLKRCGHVFC-SCLDKFKEDSSSQLSCYVCDKP 302

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BLAST of MC03g0838 vs. NCBI nr
Match: XP_022140186.1 (nitric oxide synthase-interacting protein [Momordica charantia])

HSP 1 Score: 616 bits (1588), Expect = 1.66e-222
Identity = 304/304 (100.00%), Postives = 304/304 (100.00%), Query Frame = 0


Query: 1   MPQRHSKNNNDLAFFTYDEKRKLGYGTQKERLGKDSIKPFDVCCLCLKPFIDPMCCQKGH 60
           MPQRHSKNNNDLAFFTYDEKRKLGYGTQKERLGKDSIKPFDVCCLCLKPFIDPMCCQKGH
Sbjct: 1   MPQRHSKNNNDLAFFTYDEKRKLGYGTQKERLGKDSIKPFDVCCLCLKPFIDPMCCQKGH 60

Query: 61  TFCKECILECLLAQKKDIQRKLAAYTAQQKQEKEEAEEKLMQQKARELDAFDQQNHGAVP 120
           TFCKECILECLLAQKKDIQRKLAAYTAQQKQEKEEAEEKLMQQKARELDAFDQQNHGAVP
Sbjct: 61  TFCKECILECLLAQKKDIQRKLAAYTAQQKQEKEEAEEKLMQQKARELDAFDQQNHGAVP 120

Query: 121 QYNDRNHSRDKNGFHGANSVKVTSYEEEALRNMKAFWLPSATPEAPVKAGAPSSSTFCPE 180
           QYNDRNHSRDKNGFHGANSVKVTSYEEEALRNMKAFWLPSATPEAPVKAGAPSSSTFCPE
Sbjct: 121 QYNDRNHSRDKNGFHGANSVKVTSYEEEALRNMKAFWLPSATPEAPVKAGAPSSSTFCPE 180

Query: 181 GNEKLKLKSLFPIHFTEDTSEQKKSKSYDVTYICPSCKVTLTNTMSLVALSTCGHVFCKK 240
           GNEKLKLKSLFPIHFTEDTSEQKKSKSYDVTYICPSCKVTLTNTMSLVALSTCGHVFCKK
Sbjct: 181 GNEKLKLKSLFPIHFTEDTSEQKKSKSYDVTYICPSCKVTLTNTMSLVALSTCGHVFCKK 240

Query: 241 CADKFMAVDKVCLVCNKGCKGRDLVNLEKGGTGFAAHGDSLEATDFKHLGSGSGLGLVRP 300
           CADKFMAVDKVCLVCNKGCKGRDLVNLEKGGTGFAAHGDSLEATDFKHLGSGSGLGLVRP
Sbjct: 241 CADKFMAVDKVCLVCNKGCKGRDLVNLEKGGTGFAAHGDSLEATDFKHLGSGSGLGLVRP 300

Query: 301 AMKT 304
           AMKT
Sbjct: 301 AMKT 304

[Close]

BLAST of MC03g0838 vs. NCBI nr
Match: XP_022955294.1 (E3 ubiquitin-protein ligase CSU1-like [Cucurbita moschata] >XP_022955295.1 E3 ubiquitin-protein ligase CSU1-like [Cucurbita moschata] >XP_023542722.1 E3 ubiquitin-protein ligase CSU1-like [Cucurbita pepo subsp. pepo] >XP_023542723.1 E3 ubiquitin-protein ligase CSU1-like [Cucurbita pepo subsp. pepo] >KAG6573229.1 E3 ubiquitin-protein ligase CSU1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 607 bits (1566), Expect = 3.75e-219
Identity = 299/304 (98.36%), Postives = 301/304 (99.01%), Query Frame = 0


Query: 1   MPQRHSKNNNDLAFFTYDEKRKLGYGTQKERLGKDSIKPFDVCCLCLKPFIDPMCCQKGH 60
           MPQRHSKNNNDLAFFTYDEKRKLGYGTQKERLGKDSIKPFDVCCLCLKPFIDPMCCQKGH
Sbjct: 1   MPQRHSKNNNDLAFFTYDEKRKLGYGTQKERLGKDSIKPFDVCCLCLKPFIDPMCCQKGH 60

Query: 61  TFCKECILECLLAQKKDIQRKLAAYTAQQKQEKEEAEEKLMQQKARELDAFDQQNHGAVP 120
           TFCKECILECLLAQKKDIQRKLAAYTAQQKQ+KEEAEEKLMQQKARELDAFDQQNHGAVP
Sbjct: 61  TFCKECILECLLAQKKDIQRKLAAYTAQQKQDKEEAEEKLMQQKARELDAFDQQNHGAVP 120

Query: 121 QYNDRNHSRDKNGFHGANSVKVTSYEEEALRNMKAFWLPSATPEAPVKAGAPSSSTFCPE 180
           QYNDRNHSRDKNGFHGANSVKVTSYEEEALR MKAFWLPSATPEAPVKAGAPSSSTFCPE
Sbjct: 121 QYNDRNHSRDKNGFHGANSVKVTSYEEEALRTMKAFWLPSATPEAPVKAGAPSSSTFCPE 180

Query: 181 GNEKLKLKSLFPIHFTEDTSEQKKSKSYDVTYICPSCKVTLTNTMSLVALSTCGHVFCKK 240
           GNEKLKLKSLFPIHFTED SE+KKSKSYDVTYICPSCKVTLTNTMSLVALSTCGHVFCKK
Sbjct: 181 GNEKLKLKSLFPIHFTEDNSERKKSKSYDVTYICPSCKVTLTNTMSLVALSTCGHVFCKK 240

Query: 241 CADKFMAVDKVCLVCNKGCKGRDLVNLEKGGTGFAAHGDSLEATDFKHLGSGSGLGLVRP 300
           CADKFMAVDKVCLVCNKGCKGRDLVNLEKGGTGFA HGDSLEATDFKHLGSGSGLGLVRP
Sbjct: 241 CADKFMAVDKVCLVCNKGCKGRDLVNLEKGGTGFAGHGDSLEATDFKHLGSGSGLGLVRP 300

Query: 301 AMKT 304
           AMKT
Sbjct: 301 AMKT 304

[Close]

BLAST of MC03g0838 vs. NCBI nr
Match: KAG7012402.1 (Nitric oxide synthase-interacting protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 605 bits (1559), Expect = 4.38e-218
Identity = 297/304 (97.70%), Postives = 301/304 (99.01%), Query Frame = 0


Query: 1   MPQRHSKNNNDLAFFTYDEKRKLGYGTQKERLGKDSIKPFDVCCLCLKPFIDPMCCQKGH 60
           MPQRHSKNNNDLAFFTYDEKRKLGYGTQKERLGKDSIKPFDVCCLCLKPFIDPMCCQKGH
Sbjct: 1   MPQRHSKNNNDLAFFTYDEKRKLGYGTQKERLGKDSIKPFDVCCLCLKPFIDPMCCQKGH 60

Query: 61  TFCKECILECLLAQKKDIQRKLAAYTAQQKQEKEEAEEKLMQQKARELDAFDQQNHGAVP 120
           TFCKECILECLLAQKKDIQRKLAAYTAQQKQ+KEEAEEKLMQQKARELDAFDQQNHGAVP
Sbjct: 61  TFCKECILECLLAQKKDIQRKLAAYTAQQKQDKEEAEEKLMQQKARELDAFDQQNHGAVP 120

Query: 121 QYNDRNHSRDKNGFHGANSVKVTSYEEEALRNMKAFWLPSATPEAPVKAGAPSSSTFCPE 180
           QYNDRNHSRDKNGFHGANSVKVTSYEEEALR MKAFWLPSATPEAPVKAGAPSSSTFCPE
Sbjct: 121 QYNDRNHSRDKNGFHGANSVKVTSYEEEALRTMKAFWLPSATPEAPVKAGAPSSSTFCPE 180

Query: 181 GNEKLKLKSLFPIHFTEDTSEQKKSKSYDVTYICPSCKVTLTNTMSLVALSTCGHVFCKK 240
           GNEKLKLKSLFPIHFTED SE+KKSKSY+VTY+CPSCKVTLTNTMSLVALSTCGHVFCKK
Sbjct: 181 GNEKLKLKSLFPIHFTEDNSERKKSKSYNVTYLCPSCKVTLTNTMSLVALSTCGHVFCKK 240

Query: 241 CADKFMAVDKVCLVCNKGCKGRDLVNLEKGGTGFAAHGDSLEATDFKHLGSGSGLGLVRP 300
           CADKFMAVDKVCLVCNKGCKGRDLVNLEKGGTGFA HGDSLEATDFKHLGSGSGLGLVRP
Sbjct: 241 CADKFMAVDKVCLVCNKGCKGRDLVNLEKGGTGFAGHGDSLEATDFKHLGSGSGLGLVRP 300

Query: 301 AMKT 304
           AMKT
Sbjct: 301 AMKT 304

[Close]

BLAST of MC03g0838 vs. NCBI nr
Match: XP_022994432.1 (E3 ubiquitin-protein ligase CSU1-like [Cucurbita maxima] >XP_022994433.1 E3 ubiquitin-protein ligase CSU1-like [Cucurbita maxima])

HSP 1 Score: 602 bits (1551), Expect = 7.26e-217
Identity = 296/304 (97.37%), Postives = 299/304 (98.36%), Query Frame = 0


Query: 1   MPQRHSKNNNDLAFFTYDEKRKLGYGTQKERLGKDSIKPFDVCCLCLKPFIDPMCCQKGH 60
           MPQRHSKNNNDLAFFTYDEKRKLGYGTQKERLGKDSIKPFDVCCLCLKPFIDPMCCQKGH
Sbjct: 1   MPQRHSKNNNDLAFFTYDEKRKLGYGTQKERLGKDSIKPFDVCCLCLKPFIDPMCCQKGH 60

Query: 61  TFCKECILECLLAQKKDIQRKLAAYTAQQKQEKEEAEEKLMQQKARELDAFDQQNHGAVP 120
           TFCKECILECLLAQKKDIQRKLAAYTAQQKQ+KEEAEEKL QQKARELDAFDQQNHGAVP
Sbjct: 61  TFCKECILECLLAQKKDIQRKLAAYTAQQKQDKEEAEEKLRQQKARELDAFDQQNHGAVP 120

Query: 121 QYNDRNHSRDKNGFHGANSVKVTSYEEEALRNMKAFWLPSATPEAPVKAGAPSSSTFCPE 180
           QYNDRNHS+DKNGFHGANSVKVTSYEEEALR MKAFWLPSATPEAPVKAGAPSSSTFCPE
Sbjct: 121 QYNDRNHSQDKNGFHGANSVKVTSYEEEALRTMKAFWLPSATPEAPVKAGAPSSSTFCPE 180

Query: 181 GNEKLKLKSLFPIHFTEDTSEQKKSKSYDVTYICPSCKVTLTNTMSLVALSTCGHVFCKK 240
           GNEKLKLKSLFPIHFTED SE+KK KSYDVTYICPSCKVTLTNTMSLVALSTCGHVFCKK
Sbjct: 181 GNEKLKLKSLFPIHFTEDNSERKKPKSYDVTYICPSCKVTLTNTMSLVALSTCGHVFCKK 240

Query: 241 CADKFMAVDKVCLVCNKGCKGRDLVNLEKGGTGFAAHGDSLEATDFKHLGSGSGLGLVRP 300
           CADKFMAVDKVCLVCNKGCKGRDLVNLEKGGTGFA HGDSLEATDFKHLGSGSGLGLVRP
Sbjct: 241 CADKFMAVDKVCLVCNKGCKGRDLVNLEKGGTGFAGHGDSLEATDFKHLGSGSGLGLVRP 300

Query: 301 AMKT 304
           AMKT
Sbjct: 301 AMKT 304

[Close]

BLAST of MC03g0838 vs. NCBI nr
Match: XP_038876326.1 (E3 ubiquitin-protein ligase CSU1-like [Benincasa hispida])

HSP 1 Score: 596 bits (1537), Expect = 9.89e-215
Identity = 295/304 (97.04%), Postives = 297/304 (97.70%), Query Frame = 0


Query: 1   MPQRHSKNNNDLAFFTYDEKRKLGYGTQKERLGKDSIKPFDVCCLCLKPFIDPMCCQKGH 60
           MPQRHSKNNNDLAFFTYDEKRKLGYGTQKERLGKDSIKPFDVCCLCLKPFIDPMCCQKGH
Sbjct: 1   MPQRHSKNNNDLAFFTYDEKRKLGYGTQKERLGKDSIKPFDVCCLCLKPFIDPMCCQKGH 60

Query: 61  TFCKECILECLLAQKKDIQRKLAAYTAQQKQEKEEAEEKLMQQKARELDAFDQQNHGAVP 120
           TFCK+CILECLLAQKKD QRKLAAYTAQQKQEKEEAEEKLMQQKARELDAFDQQNHGAVP
Sbjct: 61  TFCKQCILECLLAQKKDNQRKLAAYTAQQKQEKEEAEEKLMQQKARELDAFDQQNHGAVP 120

Query: 121 QYNDRNHSRDKNGFHGANSVKVTSYEEEALRNMKAFWLPSATPEAPVKAGAPSSSTFCPE 180
           QYNDRN SRDKNGFHGANSVKVTSYEEEALR MKAFWLPSATPEAPVKAGAPSSSTFCPE
Sbjct: 121 QYNDRNQSRDKNGFHGANSVKVTSYEEEALRTMKAFWLPSATPEAPVKAGAPSSSTFCPE 180

Query: 181 GNEKLKLKSLFPIHFTEDTSEQKKSKSYDVTYICPSCKVTLTNTMSLVALSTCGHVFCKK 240
           GNEKLKLKSLFPIHFTED SEQKKSKSYDVTYICPSCKVTLTNTMSLVAL+TCGHVFCKK
Sbjct: 181 GNEKLKLKSLFPIHFTEDNSEQKKSKSYDVTYICPSCKVTLTNTMSLVALTTCGHVFCKK 240

Query: 241 CADKFMAVDKVCLVCNKGCKGRDLVNLEKGGTGFAAHGDSLEATDFKHLGSGSGLGLVRP 300
           CADKFMAVDKVCLVCNKGCK RDLVNLEKGGTGFA HGDSLEA DFKHLGSGSGLGLVRP
Sbjct: 241 CADKFMAVDKVCLVCNKGCKVRDLVNLEKGGTGFAGHGDSLEARDFKHLGSGSGLGLVRP 300

Query: 301 AMKT 304
           AMKT
Sbjct: 301 AMKT 304

[Close]

BLAST of MC03g0838 vs. ExPASy TrEMBL
Match: A0A6J1CF89 (nitric oxide synthase-interacting protein OS=Momordica charantia OX=3673 GN=LOC111010915 PE=3 SV=1)

HSP 1 Score: 616 bits (1588), Expect = 8.03e-223
Identity = 304/304 (100.00%), Postives = 304/304 (100.00%), Query Frame = 0


Query: 1   MPQRHSKNNNDLAFFTYDEKRKLGYGTQKERLGKDSIKPFDVCCLCLKPFIDPMCCQKGH 60
           MPQRHSKNNNDLAFFTYDEKRKLGYGTQKERLGKDSIKPFDVCCLCLKPFIDPMCCQKGH
Sbjct: 1   MPQRHSKNNNDLAFFTYDEKRKLGYGTQKERLGKDSIKPFDVCCLCLKPFIDPMCCQKGH 60

Query: 61  TFCKECILECLLAQKKDIQRKLAAYTAQQKQEKEEAEEKLMQQKARELDAFDQQNHGAVP 120
           TFCKECILECLLAQKKDIQRKLAAYTAQQKQEKEEAEEKLMQQKARELDAFDQQNHGAVP
Sbjct: 61  TFCKECILECLLAQKKDIQRKLAAYTAQQKQEKEEAEEKLMQQKARELDAFDQQNHGAVP 120

Query: 121 QYNDRNHSRDKNGFHGANSVKVTSYEEEALRNMKAFWLPSATPEAPVKAGAPSSSTFCPE 180
           QYNDRNHSRDKNGFHGANSVKVTSYEEEALRNMKAFWLPSATPEAPVKAGAPSSSTFCPE
Sbjct: 121 QYNDRNHSRDKNGFHGANSVKVTSYEEEALRNMKAFWLPSATPEAPVKAGAPSSSTFCPE 180

Query: 181 GNEKLKLKSLFPIHFTEDTSEQKKSKSYDVTYICPSCKVTLTNTMSLVALSTCGHVFCKK 240
           GNEKLKLKSLFPIHFTEDTSEQKKSKSYDVTYICPSCKVTLTNTMSLVALSTCGHVFCKK
Sbjct: 181 GNEKLKLKSLFPIHFTEDTSEQKKSKSYDVTYICPSCKVTLTNTMSLVALSTCGHVFCKK 240

Query: 241 CADKFMAVDKVCLVCNKGCKGRDLVNLEKGGTGFAAHGDSLEATDFKHLGSGSGLGLVRP 300
           CADKFMAVDKVCLVCNKGCKGRDLVNLEKGGTGFAAHGDSLEATDFKHLGSGSGLGLVRP
Sbjct: 241 CADKFMAVDKVCLVCNKGCKGRDLVNLEKGGTGFAAHGDSLEATDFKHLGSGSGLGLVRP 300

Query: 301 AMKT 304
           AMKT
Sbjct: 301 AMKT 304

[Close]

BLAST of MC03g0838 vs. ExPASy TrEMBL
Match: A0A6J1GVU3 (E3 ubiquitin-protein ligase CSU1-like OS=Cucurbita moschata OX=3662 GN=LOC111457299 PE=3 SV=1)

HSP 1 Score: 607 bits (1566), Expect = 1.82e-219
Identity = 299/304 (98.36%), Postives = 301/304 (99.01%), Query Frame = 0


Query: 1   MPQRHSKNNNDLAFFTYDEKRKLGYGTQKERLGKDSIKPFDVCCLCLKPFIDPMCCQKGH 60
           MPQRHSKNNNDLAFFTYDEKRKLGYGTQKERLGKDSIKPFDVCCLCLKPFIDPMCCQKGH
Sbjct: 1   MPQRHSKNNNDLAFFTYDEKRKLGYGTQKERLGKDSIKPFDVCCLCLKPFIDPMCCQKGH 60

Query: 61  TFCKECILECLLAQKKDIQRKLAAYTAQQKQEKEEAEEKLMQQKARELDAFDQQNHGAVP 120
           TFCKECILECLLAQKKDIQRKLAAYTAQQKQ+KEEAEEKLMQQKARELDAFDQQNHGAVP
Sbjct: 61  TFCKECILECLLAQKKDIQRKLAAYTAQQKQDKEEAEEKLMQQKARELDAFDQQNHGAVP 120

Query: 121 QYNDRNHSRDKNGFHGANSVKVTSYEEEALRNMKAFWLPSATPEAPVKAGAPSSSTFCPE 180
           QYNDRNHSRDKNGFHGANSVKVTSYEEEALR MKAFWLPSATPEAPVKAGAPSSSTFCPE
Sbjct: 121 QYNDRNHSRDKNGFHGANSVKVTSYEEEALRTMKAFWLPSATPEAPVKAGAPSSSTFCPE 180

Query: 181 GNEKLKLKSLFPIHFTEDTSEQKKSKSYDVTYICPSCKVTLTNTMSLVALSTCGHVFCKK 240
           GNEKLKLKSLFPIHFTED SE+KKSKSYDVTYICPSCKVTLTNTMSLVALSTCGHVFCKK
Sbjct: 181 GNEKLKLKSLFPIHFTEDNSERKKSKSYDVTYICPSCKVTLTNTMSLVALSTCGHVFCKK 240

Query: 241 CADKFMAVDKVCLVCNKGCKGRDLVNLEKGGTGFAAHGDSLEATDFKHLGSGSGLGLVRP 300
           CADKFMAVDKVCLVCNKGCKGRDLVNLEKGGTGFA HGDSLEATDFKHLGSGSGLGLVRP
Sbjct: 241 CADKFMAVDKVCLVCNKGCKGRDLVNLEKGGTGFAGHGDSLEATDFKHLGSGSGLGLVRP 300

Query: 301 AMKT 304
           AMKT
Sbjct: 301 AMKT 304

[Close]

BLAST of MC03g0838 vs. ExPASy TrEMBL
Match: A0A6J1JVT4 (E3 ubiquitin-protein ligase CSU1-like OS=Cucurbita maxima OX=3661 GN=LOC111490154 PE=3 SV=1)

HSP 1 Score: 602 bits (1551), Expect = 3.51e-217
Identity = 296/304 (97.37%), Postives = 299/304 (98.36%), Query Frame = 0


Query: 1   MPQRHSKNNNDLAFFTYDEKRKLGYGTQKERLGKDSIKPFDVCCLCLKPFIDPMCCQKGH 60
           MPQRHSKNNNDLAFFTYDEKRKLGYGTQKERLGKDSIKPFDVCCLCLKPFIDPMCCQKGH
Sbjct: 1   MPQRHSKNNNDLAFFTYDEKRKLGYGTQKERLGKDSIKPFDVCCLCLKPFIDPMCCQKGH 60

Query: 61  TFCKECILECLLAQKKDIQRKLAAYTAQQKQEKEEAEEKLMQQKARELDAFDQQNHGAVP 120
           TFCKECILECLLAQKKDIQRKLAAYTAQQKQ+KEEAEEKL QQKARELDAFDQQNHGAVP
Sbjct: 61  TFCKECILECLLAQKKDIQRKLAAYTAQQKQDKEEAEEKLRQQKARELDAFDQQNHGAVP 120

Query: 121 QYNDRNHSRDKNGFHGANSVKVTSYEEEALRNMKAFWLPSATPEAPVKAGAPSSSTFCPE 180
           QYNDRNHS+DKNGFHGANSVKVTSYEEEALR MKAFWLPSATPEAPVKAGAPSSSTFCPE
Sbjct: 121 QYNDRNHSQDKNGFHGANSVKVTSYEEEALRTMKAFWLPSATPEAPVKAGAPSSSTFCPE 180

Query: 181 GNEKLKLKSLFPIHFTEDTSEQKKSKSYDVTYICPSCKVTLTNTMSLVALSTCGHVFCKK 240
           GNEKLKLKSLFPIHFTED SE+KK KSYDVTYICPSCKVTLTNTMSLVALSTCGHVFCKK
Sbjct: 181 GNEKLKLKSLFPIHFTEDNSERKKPKSYDVTYICPSCKVTLTNTMSLVALSTCGHVFCKK 240

Query: 241 CADKFMAVDKVCLVCNKGCKGRDLVNLEKGGTGFAAHGDSLEATDFKHLGSGSGLGLVRP 300
           CADKFMAVDKVCLVCNKGCKGRDLVNLEKGGTGFA HGDSLEATDFKHLGSGSGLGLVRP
Sbjct: 241 CADKFMAVDKVCLVCNKGCKGRDLVNLEKGGTGFAGHGDSLEATDFKHLGSGSGLGLVRP 300

Query: 301 AMKT 304
           AMKT
Sbjct: 301 AMKT 304

[Close]

BLAST of MC03g0838 vs. ExPASy TrEMBL
Match: A0A5A7TEE0 (Nitric oxide synthase-interacting protein-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G001520 PE=3 SV=1)

HSP 1 Score: 596 bits (1536), Expect = 6.80e-215
Identity = 295/304 (97.04%), Postives = 297/304 (97.70%), Query Frame = 0


Query: 1   MPQRHSKNNNDLAFFTYDEKRKLGYGTQKERLGKDSIKPFDVCCLCLKPFIDPMCCQKGH 60
           MPQRHSKNNNDLAFFTYDEKRKLGYGTQKERLGKDSIKPFDVCCLCLKPFIDPMCCQKGH
Sbjct: 1   MPQRHSKNNNDLAFFTYDEKRKLGYGTQKERLGKDSIKPFDVCCLCLKPFIDPMCCQKGH 60

Query: 61  TFCKECILECLLAQKKDIQRKLAAYTAQQKQEKEEAEEKLMQQKARELDAFDQQNHGAVP 120
           TFCK+CILECLLAQKKD QRKLAAYTAQQKQEKEEAEEKLMQQKARELDAFDQQNHGAVP
Sbjct: 61  TFCKQCILECLLAQKKDNQRKLAAYTAQQKQEKEEAEEKLMQQKARELDAFDQQNHGAVP 120

Query: 121 QYNDRNHSRDKNGFHGANSVKVTSYEEEALRNMKAFWLPSATPEAPVKAGAPSSSTFCPE 180
           QYNDRN SRDKNGFHGANSVKVTSYEEEALR MKAFWLPSATPEAPVKAGAPSSSTFCPE
Sbjct: 121 QYNDRNQSRDKNGFHGANSVKVTSYEEEALRTMKAFWLPSATPEAPVKAGAPSSSTFCPE 180

Query: 181 GNEKLKLKSLFPIHFTEDTSEQKKSKSYDVTYICPSCKVTLTNTMSLVALSTCGHVFCKK 240
           GNEKLKLKSLFPIHFTED SEQKKSKSYDV+YICPSCKVTLTNTMSLVALSTCGHVFCKK
Sbjct: 181 GNEKLKLKSLFPIHFTEDNSEQKKSKSYDVSYICPSCKVTLTNTMSLVALSTCGHVFCKK 240

Query: 241 CADKFMAVDKVCLVCNKGCKGRDLVNLEKGGTGFAAHGDSLEATDFKHLGSGSGLGLVRP 300
           CADKFMAVDKVCLVCNKGCK RDLVNLEKGGTGFA HGDSLEA DFKHLGSGSGLGLVRP
Sbjct: 241 CADKFMAVDKVCLVCNKGCKVRDLVNLEKGGTGFAGHGDSLEARDFKHLGSGSGLGLVRP 300

Query: 301 AMKT 304
           AMKT
Sbjct: 301 AMKT 304

[Close]

BLAST of MC03g0838 vs. ExPASy TrEMBL
Match: A0A0A0KF19 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G087940 PE=3 SV=1)

HSP 1 Score: 595 bits (1533), Expect = 1.95e-214
Identity = 294/304 (96.71%), Postives = 297/304 (97.70%), Query Frame = 0


Query: 1   MPQRHSKNNNDLAFFTYDEKRKLGYGTQKERLGKDSIKPFDVCCLCLKPFIDPMCCQKGH 60
           MPQRHSKNNNDLAFFTYDEKRKLGYGTQKERLGKDSIKPFDVCCLCLKPFIDPMCCQKGH
Sbjct: 1   MPQRHSKNNNDLAFFTYDEKRKLGYGTQKERLGKDSIKPFDVCCLCLKPFIDPMCCQKGH 60

Query: 61  TFCKECILECLLAQKKDIQRKLAAYTAQQKQEKEEAEEKLMQQKARELDAFDQQNHGAVP 120
           TFCK+CILECLLAQKKD QRKLAAYTAQQKQEKEEAEEKLMQQKARELDAFDQQNHGAVP
Sbjct: 61  TFCKQCILECLLAQKKDNQRKLAAYTAQQKQEKEEAEEKLMQQKARELDAFDQQNHGAVP 120

Query: 121 QYNDRNHSRDKNGFHGANSVKVTSYEEEALRNMKAFWLPSATPEAPVKAGAPSSSTFCPE 180
           QYNDRN +RDKNGFHGANSVKVTSYEEEALR MKAFWLPSATPEAPVKAGAPSSSTFCPE
Sbjct: 121 QYNDRNQNRDKNGFHGANSVKVTSYEEEALRTMKAFWLPSATPEAPVKAGAPSSSTFCPE 180

Query: 181 GNEKLKLKSLFPIHFTEDTSEQKKSKSYDVTYICPSCKVTLTNTMSLVALSTCGHVFCKK 240
           GNEKLKLKSLFPIHFTED SE+KKSKSYDVTYICPSCKVTLTNTMSLVALSTCGHVFCKK
Sbjct: 181 GNEKLKLKSLFPIHFTEDNSERKKSKSYDVTYICPSCKVTLTNTMSLVALSTCGHVFCKK 240

Query: 241 CADKFMAVDKVCLVCNKGCKGRDLVNLEKGGTGFAAHGDSLEATDFKHLGSGSGLGLVRP 300
           CADKFMAVDKVCLVCNKGCK RDLVNLEKGGTGFA HGDSLEA DFKHLGSGSGLGLVRP
Sbjct: 241 CADKFMAVDKVCLVCNKGCKVRDLVNLEKGGTGFAGHGDSLEARDFKHLGSGSGLGLVRP 300

Query: 301 AMKT 304
           AMKT
Sbjct: 301 AMKT 304

[Close]

BLAST of MC03g0838 vs. TAIR 10
Match: AT1G61620.1 (phosphoinositide binding )

HSP 1 Score: 481.9 bits (1239), Expect = 3.9e-136
Identity = 233/307 (75.90%), Postives = 267/307 (86.97%), Query Frame = 0


Query: 1   MPQRHSKNNNDLAFFTYDEKRKLGYGTQKERLGKDSIKPFDVCCLCLKPFIDPMCCQKGH 60
           MPQRHSKNNNDLA+FTYDEK+KLGYGTQ+ERLG+DSIKPFD C LCLKPFIDPMCC KGH
Sbjct: 1   MPQRHSKNNNDLAYFTYDEKKKLGYGTQRERLGRDSIKPFDACSLCLKPFIDPMCCHKGH 60

Query: 61  TFCKECILECLLAQKKDIQRKLAAYTAQQKQEKEEAEEKLMQQKARELDAFDQQNHGAVP 120
            FC+ECILEC LAQKKDIQR+LAA+++Q+KQ+K+E EE+LM QKARELD FDQQNH A+P
Sbjct: 61  VFCRECILECFLAQKKDIQRRLAAHSSQKKQDKDEEEERLMLQKARELDEFDQQNHSAMP 120

Query: 121 QYNDRNHSRDKNGFHGANSVKVTSYEEEALRNMKAFWLPSATPEAPVKAGAPSSSTFCPE 180
           + +D+NH+ DKNGFHGANSVK TS+EEEALR MKAFWLPSATP A V+  AP + T CPE
Sbjct: 121 RNSDKNHNEDKNGFHGANSVKTTSFEEEALRTMKAFWLPSATPAASVRVDAPETHTVCPE 180

Query: 181 GNEKLKLKSLFPIHFTEDTSEQKKSK-------SYDVTYICPSCKVTLTNTMSLVALSTC 240
           G EKLKLK+LF I FTED SE++++K       SYD +YICPSCKVTLTNTMSLVALS+C
Sbjct: 181 GKEKLKLKNLFAIRFTEDNSEEEETKTKSASSSSYDKSYICPSCKVTLTNTMSLVALSSC 240

Query: 241 GHVFCKKCADKFMAVDKVCLVCNKGCKGRDLVNLEKGGTGFAAHGDSLEATDFKHLGSGS 300
           GHVFCKKCA+KFM VDKVCLVC+K CK R+LV L+KGGTGFA H D LEA ++KHLGSGS
Sbjct: 241 GHVFCKKCAEKFMPVDKVCLVCDKPCKDRNLVGLKKGGTGFAEHDDHLEAKEYKHLGSGS 300

The following BLAST results are available for this feature:

BLAST of MC03g0838 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SY88	5.4e-135	75.90	E3 ubiquitin-protein ligase CSU1 OS=Arabidopsis thaliana OX=3702 GN=CSU1 PE=1 SV...	[more]
Q5U3S7	2.1e-33	33.22	Nitric oxide synthase-interacting protein OS=Danio rerio OX=7955 GN=nosip PE=2 S...	[more]
Q6P829	2.1e-33	32.87	Nitric oxide synthase-interacting protein OS=Xenopus tropicalis OX=8364 GN=nosip...	[more]
Q6NUH3	2.7e-33	32.87	Nitric oxide synthase-interacting protein OS=Xenopus laevis OX=8355 GN=nosip PE=...	[more]
Q55DU4	2.3e-32	31.52	Nitric oxide synthase-interacting protein homolog OS=Dictyostelium discoideum OX...	[more]

BLAST of MC03g0838 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022140186.1	1.66e-222	100.00	nitric oxide synthase-interacting protein [Momordica charantia]	[more]
XP_022955294.1	3.75e-219	98.36	E3 ubiquitin-protein ligase CSU1-like [Cucurbita moschata] >XP_022955295.1 E3 ub...	[more]
KAG7012402.1	4.38e-218	97.70	Nitric oxide synthase-interacting protein [Cucurbita argyrosperma subsp. argyros...	[more]
XP_022994432.1	7.26e-217	97.37	E3 ubiquitin-protein ligase CSU1-like [Cucurbita maxima] >XP_022994433.1 E3 ubiq...	[more]
XP_038876326.1	9.89e-215	97.04	E3 ubiquitin-protein ligase CSU1-like [Benincasa hispida]	[more]

BLAST of MC03g0838 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CF89	8.03e-223	100.00	nitric oxide synthase-interacting protein OS=Momordica charantia OX=3673 GN=LOC1...	[more]
A0A6J1GVU3	1.82e-219	98.36	E3 ubiquitin-protein ligase CSU1-like OS=Cucurbita moschata OX=3662 GN=LOC111457...	[more]
A0A6J1JVT4	3.51e-217	97.37	E3 ubiquitin-protein ligase CSU1-like OS=Cucurbita maxima OX=3661 GN=LOC11149015...	[more]
A0A5A7TEE0	6.80e-215	97.04	Nitric oxide synthase-interacting protein-like OS=Cucumis melo var. makuwa OX=11...	[more]
A0A0A0KF19	1.95e-214	96.71	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G087940 P...	[more]

BLAST of MC03g0838 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G61620.1	3.9e-136	75.90	phosphoinositide binding	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 68..107
None	No IPR available	PFAM	PF04641	Rtf2	coord: 186..268 e-value: 1.7E-9 score: 37.5
None	No IPR available	CDD	cd16513	RING1-HC_LONFs	coord: 214..256 e-value: 3.093E-12 score: 58.2305
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 25..76
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 213..259
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 214..255 e-value: 0.046 score: 22.8 coord: 43..73 e-value: 7.9 score: 3.6
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 214..256 score: 10.232069
IPR016818	Nitric oxide synthase-interacting protein	PIRSF	PIRSF023577	NOSIP	coord: 2..292 e-value: 2.1E-97 score: 324.1
IPR016818	Nitric oxide synthase-interacting protein	PANTHER	PTHR13063	ENOS INTERACTING PROTEIN	coord: 1..291
IPR031790	Nitric oxide synthase-interacting protein, zinc-finger	PFAM	PF15906	zf-NOSIP	coord: 5..75 e-value: 1.5E-15 score: 57.1
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 16..135 e-value: 2.3E-8 score: 36.0
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 187..270 e-value: 3.5E-9 score: 38.4
IPR017907	Zinc finger, RING-type, conserved site	PROSITE	PS00518	ZF_RING_1	coord: 233..242

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC03g0838.1	MC03g0838.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0005634	nucleus
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0061630	ubiquitin protein ligase activity