MC03g0833 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC03g0833
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionNADH:ubiquinone reductase (non-electrogenic)
LocationMC03: 14961359 .. 14965721 (+)
RNA-Seq ExpressionMC03g0833
SyntenyMC03g0833
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTCTTTTTTTCTGCAGTGGGGGTAGCCTTGTAGCATATGCTGATGCTAGTCCAATAAATGTTGCACCCAGCACCAGCATTGCATCATTTAAAGCGGACGTCGATGAAAAAAAGAAGAAGGTGGTAGTACTTGGAACTGGTTGGGCCGGAACAAGTTTCTTGAAGAACATTAAAGATGCCTCATATGAGATTCAAGTGATATCCCCTCGTAATTACTTTGCATTCACCCCATTATTGCCAAGTGTTACATGTGGTACAGTGGAAGCCCGCAGCATTGTTGAACCGATTCGCAACATTGTGAGGAAGGTAATGGAATTCTGTCCATTTGGCTATGTGCTGTAAGAGTTGGAACATATAATTGTAGAACTAGTTGGCTATGTTCAAAGAGTTTTTTGGAGGAAAAAATGTAGAAAGATTTCCATATCTTATCAATAAACGGTAAGAAAATACGACGTTACTTGAACAAGATCTGCTCTATAGGTTGTACTATTGTTTCCTTGAGTCCTAAGGAGAAAAATTTAATAAACAGTAAGAAAATGAGTAATGCCATTTTTGTAGACAGTTGGCTTTCCTCTCATGTAACTTCTATACCTAGTTACAGAAAAGGGTAATAAATGATCCTTAGAAAAACAAATTTACTTCGTATGTAATTTTAAGATCCATTTCAAGGAGATTATGGGCCTACTTTCCACCTATTCCCTCACATGTTAGTGCTTTTACTGTAGCTTGATTATTTATTAACATTAAGATGATCTTCTTGTTTTTCCTTCTTACAGAAAGGGGTAGACATCATATTCAATGAAGCGGAATGTTACAAGATTGATGCTGAAAATAGAAAGCTCTATTGTCGATCTAATGCAAATAACAATCTGAATGGGAAAAAGGAATTTGTTGTGGACTACGACTACCTAGTGATAGCTGTGGGAGCACAAGTTAATACTTTCAATACTCCTGGTGTCGTGGAGAATTGCCACTTCTTGAAGGTAACATGCTTTATCGCCTGAACAAATTACAATTACTGATTAAAGAAGTGGCTTCTCATCAACTCTTTTTTACGTAGTATCATCATGTCGTTATAGAAGTAATAGTAGAACTAGTAATAATAATATTGGGTATAATGTATATCCCCCTTCCCTAACGGGGTTAGTAAAAACGACCACCAGTTTCACTAGGGAATGAAGAATGAAAAATCAGTGAAAGTAACTGGATCTTTTGTGAAGTACTGACTTCCTCCGCGTCTGTTACTTCTGACTAATTTCTGAGAATTGCTCTAGGAAGTGGAAGATGCTCAGAGAATACGAAGAACTGTAATTGATTGCTTTGAGAGGGCAAGTCTACCTACCCTTAATGAAGAAGATAGAAAGAGAATTCTTCACTTTGCTGTTGTTGGTGGTGGCCCAACTGGTGTGGAATTCGCTGCAGAACTTCATGATTTTGTCAATGAAGACTTGGTCAAATTATATCCTGGACTAAAAGATTTTGTAAAAATTACTCTTCTCGAGGCAGGGGATCATATTTTGAACATGTAAGGAAGTGTGACATTGAGCCTTTGAAAATTTTCTCTACTTAGTGCTTAAATATTTTTCAATGATTTTGTATCTGCCTGAGTCCTATCATACCATTTGTGCAAAAGCAACAATGAAAGATTTGTGCTGCAGGACCATTGAAATGAAGCTTTCCAATGCCTTGAATGGAAAAGCTTTATTCACAGACTTCTATATTAAATAAAACTTATGTCGTTGTAGGTTCGACAAAAGAATTACAACTTTTGCTGAAGAAAAATTCCGAAGAGATGGTATTGATGTTAAGACAGGATCAATTGTGATCAAGGTAACTGATAAAGAGATTTCTACCAAAGAAATGAAGAGTGGAGAAGTATCTTCCATGCCCTATGGCATGACAGTATGGTCAACCGGTATTGGAACTCGTCCAATCATAAAGGACTTTATGACACAGATTGGTCAGGTTAGTTTATTGGAAGCCTCTAACATACTAATATTGCAGGTCTGACATAATACTATTTGGTTGCTGTCTCCTCTCTCACCATTTTACTTTACAAAATTTGCTCTGCTCTCTTTTTCAGCCTCTCTCACATTTATAAGCGATATTTGTATAATGTGTATAGGTTAACAGGCGTGCTTTAGCAACCGATGAATGGCTTCGAGTTGAGGGATGTGACAATGTATATGCAATTGGTGATAGTGCCACCATAAATCAGCGAAGAGTCATGGTGAGTGTACTCGTCCCTTTTGAAAGTCTTACGGTCATTTTTGGCTCATGTCCACTGGCTCTCTTTCAAGTTTCTATACCCATGATTCATCGTACCATGGTTTTTCATGTTTTAGTTTGATGAACACTTATTAATTTAATATTTTATAAACCAAATTATCATATATATTTTTATTTTTTGTGATTATTCGATTTGTTTGATCTTATTTCTCCCTTTCCTGCTCGCTGTTGCTAATTTCTCAACAGGAAGATATTGCAGCAATTTTTAGCAAGGCAGACAAAGACAATTCTGGGACACTAACTGTAAAAGAATTTCAAGAAGTCATTAATGACATATGTGAAAGATATCCCCAGGTGGAGCTGTATTTGAAGAATAAGCAGATGCACAACATCGTTGATCTTTTGAAAGGATCCAAAGGGGATGTGGCAAAGGAGTCCATCGAATTAAACATTGAAGAGTTTAAATTAGCTCTTTCCCAAGTCGATTCTCAAATGAAAAATCTTCCTGCCACCGCACAGGTCCGCCATAGATTCTTGCATGATACCCATTTGATACTGAAATTGGATTTCCACAATAAACATGTTTTACACCCATCTGAAATGTCGTTTCAGGTGGCTGCCCAGCAAGGTGCATATCTTGCCAAATGTTTTAACCGCATGGAAGAGTGCGAACAAAATCCTGAAGGTCCTCTTAGGTTTAGGGGAACAGGACGCCACCGTTTTCGGCCCTTTAGGTACCACTCTCTCTCTCTCTTTTGATCTGATGTGTGCAATAGCCGAATCAAAGATAAAACGCGAACATTTTTGCATGTGAATCCCGTAAAGTTCGGCAGGGGTTAATAAAACTAGATAAGTTTCTTTTGTATTAAAAAAAATGAAAGTAAAATAATTTGACCATCTAGACGTGTGAATTCATTCTCAATAAATAGTTCAACCACTCATTTTGTTATTGAGTCTACTGGGAGTATGGGACCTCTTTCCTTATTGCTACATGCAAGCGTCCTGCTGAACTGTTATGAATGCAGGCTCGCTTATCGAAATAAAATCATTATCTTTCTTTAGGTCTATAGAACAGGGGAGGCAGATATTACAGTCTGATGCAGTTGTGCACAGTGACATTCTTGACATTATTGCACCGAACTTCTAGACAATTCAATTTCAAATTTGAACTTTCTGAATGTAAGGAACTAATATGAGTCTGATTTGCTCTCTGTGTGCGACCGTCCTTGAAGGTACAAACATCTTGGACAATTTGCACCGTTGGGAGGAGAACAAACGGCTGCACAACTTCCTGGGGATTGGGTGTCCATTGGCCACAGCTCTCAGTGGCTCTGGTACTCTGTCTATGCAAGGTGACATTCTAAAGGAAATGAAATTTATCTTTATATAATAAGAAAAATTGATTCAGTAACTTAATACCAAAAATCTTTGATGTCTCCCTCGAGTGTTGGGTGGGTGTCCTTGGGTATAGGGGAATTAAGCTCCACCCCAGTTATCAAACGAAAAAAATAAGGAAAATAGATTAGTTTAACTTCAGATTAGGATATGCTTTGTCTTGACTGTTTGGTGATTCTCTCATTTGTTTCAGTAAGCAAGTTAGTTGGCGGACAAGGGCATTAGTGGTTACAGATTGGACGAGACGTTTCGTTTTTGGCAGGGATTCGAGTCGCATATGAATCAACCCAAGACCGGGAGTTGGTATTTTAGTTTGAAGATGAAGGATGGTTGGCTGCTCAAGTTTAAAGCAAGTGCAGCATTGCGAGTTAATGATACACACAAAGTTTAGTTTACTTTATTCATCAAGGAAAATACATAAATCTCGGCAGAGTGACCAATATAGTTGGTACAATAATTTTGGTCATATTCGTCCGATGAATTTGTTAGTTCATTGTGGTCAGCAGTGAGCTTTTTTGTCAATGCTTCTTCCCCCTCCTCCGCGGCTTAAGATGTGAATTCTTGTTTTGAGTTTGAGCATATTATGATTTATTGATACAAAGGCCAGAGTTGATGCAAATTTATCTCTCTCCCGATAATTATGATATTGTTCTCAACCTATCAAGTTTAGTACGACCTCCAAGCAAAGGTTGTTGGCTAAATAAATGTTTATAAAATTAATCTCAAATCTATTGCTTCG

mRNA sequence

GTTCTTTTTTTCTGCAGTGGGGGTAGCCTTGTAGCATATGCTGATGCTAGTCCAATAAATGTTGCACCCAGCACCAGCATTGCATCATTTAAAGCGGACGTCGATGAAAAAAAGAAGAAGGTGGTAGTACTTGGAACTGGTTGGGCCGGAACAAGTTTCTTGAAGAACATTAAAGATGCCTCATATGAGATTCAAGTGATATCCCCTCGTAATTACTTTGCATTCACCCCATTATTGCCAAGTGTTACATGTGGTACAGTGGAAGCCCGCAGCATTGTTGAACCGATTCGCAACATTGTGAGGAAGAAAGGGGTAGACATCATATTCAATGAAGCGGAATGTTACAAGATTGATGCTGAAAATAGAAAGCTCTATTGTCGATCTAATGCAAATAACAATCTGAATGGGAAAAAGGAATTTGTTGTGGACTACGACTACCTAGTGATAGCTGTGGGAGCACAAGTTAATACTTTCAATACTCCTGGTGTCGTGGAGAATTGCCACTTCTTGAAGGAAGTGGAAGATGCTCAGAGAATACGAAGAACTGTAATTGATTGCTTTGAGAGGGCAAGTCTACCTACCCTTAATGAAGAAGATAGAAAGAGAATTCTTCACTTTGCTGTTGTTGGTGGTGGCCCAACTGGTGTGGAATTCGCTGCAGAACTTCATGATTTTGTCAATGAAGACTTGGTCAAATTATATCCTGGACTAAAAGATTTTGTAAAAATTACTCTTCTCGAGGCAGGGGATCATATTTTGAACATGTTCGACAAAAGAATTACAACTTTTGCTGAAGAAAAATTCCGAAGAGATGGTATTGATGTTAAGACAGGATCAATTGTGATCAAGGTAACTGATAAAGAGATTTCTACCAAAGAAATGAAGAGTGGAGAAGTATCTTCCATGCCCTATGGCATGACAGTATGGTCAACCGGTATTGGAACTCGTCCAATCATAAAGGACTTTATGACACAGATTGGTCAGGTTAACAGGCGTGCTTTAGCAACCGATGAATGGCTTCGAGTTGAGGGATGTGACAATGTATATGCAATTGGTGATAGTGCCACCATAAATCAGCGAAGAGTCATGGAAGATATTGCAGCAATTTTTAGCAAGGCAGACAAAGACAATTCTGGGACACTAACTGTAAAAGAATTTCAAGAAGTCATTAATGACATATGTGAAAGATATCCCCAGGTGGAGCTGTATTTGAAGAATAAGCAGATGCACAACATCGTTGATCTTTTGAAAGGATCCAAAGGGGATGTGGCAAAGGAGTCCATCGAATTAAACATTGAAGAGTTTAAATTAGCTCTTTCCCAAGTCGATTCTCAAATGAAAAATCTTCCTGCCACCGCACAGGTGGCTGCCCAGCAAGGTGCATATCTTGCCAAATGTTTTAACCGCATGGAAGAGTGCGAACAAAATCCTGAAGGTCCTCTTAGGTTTAGGGGAACAGGACGCCACCGTTTTCGGCCCTTTAGGTACAAACATCTTGGACAATTTGCACCGTTGGGAGGAGAACAAACGGCTGCACAACTTCCTGGGGATTGGGTGTCCATTGGCCACAGCTCTCAGTGGCTCTGGTACTCTGTCTATGCAAGTAAGCAAGTTAGTTGGCGGACAAGGGCATTAGTGGTTACAGATTGGACGAGACGTTTCGTTTTTGGCAGGGATTCGAGTCGCATATGAATCAACCCAAGACCGGGAGTTGGTATTTTAGTTTGAAGATGAAGGATGGTTGGCTGCTCAAGTTTAAAGCAAGTGCAGCATTGCGAGTTAATGATACACACAAAGTTTAGTTTACTTTATTCATCAAGGAAAATACATAAATCTCGGCAGAGTGACCAATATAGTTGGTACAATAATTTTGGTCATATTCGTCCGATGAATTTGTTAGTTCATTGTGGTCAGCAGTGAGCTTTTTTGTCAATGCTTCTTCCCCCTCCTCCGCGGCTTAAGATGTGAATTCTTGTTTTGAGTTTGAGCATATTATGATTTATTGATACAAAGGCCAGAGTTGATGCAAATTTATCTCTCTCCCGATAATTATGATATTGTTCTCAACCTATCAAGTTTAGTACGACCTCCAAGCAAAGGTTGTTGGCTAAATAAATGTTTATAAAATTAATCTCAAATCTATTGCTTCG

Coding sequence (CDS)

GTTCTTTTTTTCTGCAGTGGGGGTAGCCTTGTAGCATATGCTGATGCTAGTCCAATAAATGTTGCACCCAGCACCAGCATTGCATCATTTAAAGCGGACGTCGATGAAAAAAAGAAGAAGGTGGTAGTACTTGGAACTGGTTGGGCCGGAACAAGTTTCTTGAAGAACATTAAAGATGCCTCATATGAGATTCAAGTGATATCCCCTCGTAATTACTTTGCATTCACCCCATTATTGCCAAGTGTTACATGTGGTACAGTGGAAGCCCGCAGCATTGTTGAACCGATTCGCAACATTGTGAGGAAGAAAGGGGTAGACATCATATTCAATGAAGCGGAATGTTACAAGATTGATGCTGAAAATAGAAAGCTCTATTGTCGATCTAATGCAAATAACAATCTGAATGGGAAAAAGGAATTTGTTGTGGACTACGACTACCTAGTGATAGCTGTGGGAGCACAAGTTAATACTTTCAATACTCCTGGTGTCGTGGAGAATTGCCACTTCTTGAAGGAAGTGGAAGATGCTCAGAGAATACGAAGAACTGTAATTGATTGCTTTGAGAGGGCAAGTCTACCTACCCTTAATGAAGAAGATAGAAAGAGAATTCTTCACTTTGCTGTTGTTGGTGGTGGCCCAACTGGTGTGGAATTCGCTGCAGAACTTCATGATTTTGTCAATGAAGACTTGGTCAAATTATATCCTGGACTAAAAGATTTTGTAAAAATTACTCTTCTCGAGGCAGGGGATCATATTTTGAACATGTTCGACAAAAGAATTACAACTTTTGCTGAAGAAAAATTCCGAAGAGATGGTATTGATGTTAAGACAGGATCAATTGTGATCAAGGTAACTGATAAAGAGATTTCTACCAAAGAAATGAAGAGTGGAGAAGTATCTTCCATGCCCTATGGCATGACAGTATGGTCAACCGGTATTGGAACTCGTCCAATCATAAAGGACTTTATGACACAGATTGGTCAGGTTAACAGGCGTGCTTTAGCAACCGATGAATGGCTTCGAGTTGAGGGATGTGACAATGTATATGCAATTGGTGATAGTGCCACCATAAATCAGCGAAGAGTCATGGAAGATATTGCAGCAATTTTTAGCAAGGCAGACAAAGACAATTCTGGGACACTAACTGTAAAAGAATTTCAAGAAGTCATTAATGACATATGTGAAAGATATCCCCAGGTGGAGCTGTATTTGAAGAATAAGCAGATGCACAACATCGTTGATCTTTTGAAAGGATCCAAAGGGGATGTGGCAAAGGAGTCCATCGAATTAAACATTGAAGAGTTTAAATTAGCTCTTTCCCAAGTCGATTCTCAAATGAAAAATCTTCCTGCCACCGCACAGGTGGCTGCCCAGCAAGGTGCATATCTTGCCAAATGTTTTAACCGCATGGAAGAGTGCGAACAAAATCCTGAAGGTCCTCTTAGGTTTAGGGGAACAGGACGCCACCGTTTTCGGCCCTTTAGGTACAAACATCTTGGACAATTTGCACCGTTGGGAGGAGAACAAACGGCTGCACAACTTCCTGGGGATTGGGTGTCCATTGGCCACAGCTCTCAGTGGCTCTGGTACTCTGTCTATGCAAGTAAGCAAGTTAGTTGGCGGACAAGGGCATTAGTGGTTACAGATTGGACGAGACGTTTCGTTTTTGGCAGGGATTCGAGTCGCATATGA

Protein sequence

VLFFCSGGSLVAYADASPINVAPSTSIASFKADVDEKKKKVVVLGTGWAGTSFLKNIKDASYEIQVISPRNYFAFTPLLPSVTCGTVEARSIVEPIRNIVRKKGVDIIFNEAECYKIDAENRKLYCRSNANNNLNGKKEFVVDYDYLVIAVGAQVNTFNTPGVVENCHFLKEVEDAQRIRRTVIDCFERASLPTLNEEDRKRILHFAVVGGGPTGVEFAAELHDFVNEDLVKLYPGLKDFVKITLLEAGDHILNMFDKRITTFAEEKFRRDGIDVKTGSIVIKVTDKEISTKEMKSGEVSSMPYGMTVWSTGIGTRPIIKDFMTQIGQVNRRALATDEWLRVEGCDNVYAIGDSATINQRRVMEDIAAIFSKADKDNSGTLTVKEFQEVINDICERYPQVELYLKNKQMHNIVDLLKGSKGDVAKESIELNIEEFKLALSQVDSQMKNLPATAQVAAQQGAYLAKCFNRMEECEQNPEGPLRFRGTGRHRFRPFRYKHLGQFAPLGGEQTAAQLPGDWVSIGHSSQWLWYSVYASKQVSWRTRALVVTDWTRRFVFGRDSSRI
Homology
BLAST of MC03g0833 vs. ExPASy Swiss-Prot
Match: F4JJJ3 (External alternative NAD(P)H-ubiquinone oxidoreductase B3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NDB3 PE=2 SV=1)

HSP 1 Score: 868.2 bits (2242), Expect = 5.0e-251
Identity = 424/559 (75.85%), Postives = 482/559 (86.23%), Query Frame = 0

Query: 6   SGGSLVAYADASPINVAPSTSIASFKADVDEKKKKVVVLGTGWAGTSFLKNIKDASYEIQ 65
           SGG L+ Y++A+P       S ++   +   +K+KVV+LGTGWAG SFLK + ++SYE+Q
Sbjct: 30  SGGGLIVYSEANP-------SYSNNGVETKTRKRKVVLLGTGWAGASFLKTLNNSSYEVQ 89

Query: 66  VISPRNYFAFTPLLPSVTCGTVEARSIVEPIRNIVRKKGVDIIFNEAECYKIDAENRKLY 125
           VISPRNYFAFTPLLPSVTCGTVEARS+VEPIRNI RK+ V++ F EAEC+KID  ++K+Y
Sbjct: 90  VISPRNYFAFTPLLPSVTCGTVEARSVVEPIRNIARKQNVEMSFLEAECFKIDPGSKKVY 149

Query: 126 CRSNANNNLNGKKEFVVDYDYLVIAVGAQVNTFNTPGVVENCHFLKEVEDAQRIRRTVID 185
           CRS    N  GKKEF VDYDYLVIA GAQ NTFN PGV ENCHFLKEVEDAQRIR TVID
Sbjct: 150 CRSKQGVNSKGKKEFDVDYDYLVIATGAQSNTFNIPGVEENCHFLKEVEDAQRIRSTVID 209

Query: 186 CFERASLPTLNEEDRKRILHFAVVGGGPTGVEFAAELHDFVNEDLVKLYPGLKDFVKITL 245
            FE+ASLP LNE++RKR+LHF VVGGGPTGVEFA+ELHDFVNEDLVKLYP  K+ V+ITL
Sbjct: 210 SFEKASLPGLNEQERKRMLHFVVVGGGPTGVEFASELHDFVNEDLVKLYPKAKNLVQITL 269

Query: 246 LEAGDHILNMFDKRITTFAEEKFRRDGIDVKTGSIVIKVTDKEISTKEMKSGEVSSMPYG 305
           LEA DHIL MFDKRIT FAEEKF RDGIDVK GS+V+KV DKEIS K  K+GEVS++PYG
Sbjct: 270 LEAADHILTMFDKRITEFAEEKFTRDGIDVKLGSMVVKVNDKEISAK-TKAGEVSTIPYG 329

Query: 306 MTVWSTGIGTRPIIKDFMTQIGQVNRRALATDEWLRVEGCDNVYAIGDSATINQRRVMED 365
           M VWSTGIGTRP+IKDFM QIGQ NRRALATDEWLRVEGCDN+YA+GD ATINQR+VMED
Sbjct: 330 MIVWSTGIGTRPVIKDFMKQIGQGNRRALATDEWLRVEGCDNIYALGDCATINQRKVMED 389

Query: 366 IAAIFSKADKDNSGTLTVKEFQEVINDICERYPQVELYLKNKQMHNIVDLLKGSKGD-VA 425
           IAAIF KADK+NSGTLT+KEF EV++DIC+RYPQVELYLK+K MH I DLLK ++ +  +
Sbjct: 390 IAAIFKKADKENSGTLTMKEFHEVMSDICDRYPQVELYLKSKGMHGITDLLKQAQAENGS 449

Query: 426 KESIELNIEEFKLALSQVDSQMKNLPATAQVAAQQGAYLAKCFNRMEECEQNPEGPLRFR 485
            +S+EL+IEE K AL QVDSQ+K LPAT QVAAQQG YLAKCF+RME CE+NPEGP+R R
Sbjct: 450 NKSVELDIEELKSALCQVDSQVKLLPATGQVAAQQGTYLAKCFDRMEVCEKNPEGPIRIR 509

Query: 486 GTGRHRFRPFRYKHLGQFAPLGGEQTAAQLPGDWVSIGHSSQWLWYSVYASKQVSWRTRA 545
           G GRHRFRPFRY+HLGQFAPLGGEQTAAQLPGDWVSIGHSSQWLWYSVYASKQVSWRTR 
Sbjct: 510 GEGRHRFRPFRYRHLGQFAPLGGEQTAAQLPGDWVSIGHSSQWLWYSVYASKQVSWRTRV 569

Query: 546 LVVTDWTRRFVFGRDSSRI 564
           LVV+DW RRF+FGRDSSRI
Sbjct: 570 LVVSDWMRRFIFGRDSSRI 580

BLAST of MC03g0833 vs. ExPASy Swiss-Prot
Match: Q94BV7 (External alternative NAD(P)H-ubiquinone oxidoreductase B2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NDB2 PE=1 SV=1)

HSP 1 Score: 860.9 bits (2223), Expect = 8.0e-249
Identity = 427/559 (76.39%), Postives = 480/559 (85.87%), Query Frame = 0

Query: 6   SGGSLVAYADASPINVAPSTSIASFKADVDEKKKKVVVLGTGWAGTSFLKNIKDASYEIQ 65
           SGG L+AY++A+    A   ++     +   KKKKVV+LGTGWAGTSFLKN+ ++ YE+Q
Sbjct: 30  SGGGLIAYSEANASYGANGGAV----VETGTKKKKVVLLGTGWAGTSFLKNLNNSQYEVQ 89

Query: 66  VISPRNYFAFTPLLPSVTCGTVEARSIVEPIRNIVRKKGVDIIFNEAECYKIDAENRKLY 125
           +ISPRNYFAFTPLLPSVTCGTVEARS+VEPIRNI R K VD  + EAEC+KID  ++K+Y
Sbjct: 90  IISPRNYFAFTPLLPSVTCGTVEARSVVEPIRNIGR-KNVDTSYLEAECFKIDPASKKVY 149

Query: 126 CRSNANNNLNGKKEFVVDYDYLVIAVGAQVNTFNTPGVVENCHFLKEVEDAQRIRRTVID 185
           CRS    + NGKKEF VDYDYLVIA GAQ NTFN PGV ENCHFLKEVEDAQRIR+TVID
Sbjct: 150 CRSKQGLSSNGKKEFSVDYDYLVIATGAQSNTFNIPGVEENCHFLKEVEDAQRIRKTVID 209

Query: 186 CFERASLPTLNEEDRKRILHFAVVGGGPTGVEFAAELHDFVNEDLVKLYPGLKDFVKITL 245
            FE+ASLP L++E+RKRILHF VVGGGPTGVEFAAELHDFV EDLV LYP  K  V+ITL
Sbjct: 210 SFEKASLPELSDEERKRILHFVVVGGGPTGVEFAAELHDFVTEDLVSLYPRAKGSVRITL 269

Query: 246 LEAGDHILNMFDKRITTFAEEKFRRDGIDVKTGSIVIKVTDKEISTKEMKSGEVSSMPYG 305
           LEA DHIL MFDKRIT FAEEKF RDGIDVK GS+V KV +K+IS K  K GEVSS+PYG
Sbjct: 270 LEAADHILTMFDKRITEFAEEKFSRDGIDVKLGSMVTKVNEKDISAK-TKGGEVSSIPYG 329

Query: 306 MTVWSTGIGTRPIIKDFMTQIGQVNRRALATDEWLRVEGCDNVYAIGDSATINQRRVMED 365
           M VWSTGIGTRP+IKDFM QIGQ NRRALATDEWLRVEG DN+YA+GD ATINQR+VMED
Sbjct: 330 MIVWSTGIGTRPVIKDFMKQIGQGNRRALATDEWLRVEGTDNIYALGDCATINQRKVMED 389

Query: 366 IAAIFSKADKDNSGTLTVKEFQEVINDICERYPQVELYLKNKQMHNIVDLLKGSK-GDVA 425
           ++AIFSKADKD SGTLT+KEFQE ++DIC RYPQVELYLK+K+M  I DLLK ++  DV+
Sbjct: 390 VSAIFSKADKDKSGTLTLKEFQEAMDDICVRYPQVELYLKSKRMRGIADLLKEAETDDVS 449

Query: 426 KESIELNIEEFKLALSQVDSQMKNLPATAQVAAQQGAYLAKCFNRMEECEQNPEGPLRFR 485
           K +IEL IEEFK ALSQVDSQ+K LPATAQVAAQQGAYLAKCF+RMEECE++PEGP+R R
Sbjct: 450 KNNIELKIEEFKSALSQVDSQVKFLPATAQVAAQQGAYLAKCFDRMEECEKSPEGPIRMR 509

Query: 486 GTGRHRFRPFRYKHLGQFAPLGGEQTAAQLPGDWVSIGHSSQWLWYSVYASKQVSWRTRA 545
           G GRHRFRPFRY+HLGQFAPLGGEQTAAQLPGDWVSIGHSSQWLWYSVYASKQVSWRTR 
Sbjct: 510 GEGRHRFRPFRYRHLGQFAPLGGEQTAAQLPGDWVSIGHSSQWLWYSVYASKQVSWRTRV 569

Query: 546 LVVTDWTRRFVFGRDSSRI 564
           LVV+DW RRF+FGRDSS I
Sbjct: 570 LVVSDWMRRFIFGRDSSSI 582

BLAST of MC03g0833 vs. ExPASy Swiss-Prot
Match: Q9SKT7 (External alternative NAD(P)H-ubiquinone oxidoreductase B4, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NDB4 PE=1 SV=1)

HSP 1 Score: 807.4 bits (2084), Expect = 1.0e-232
Identity = 387/560 (69.11%), Postives = 463/560 (82.68%), Query Frame = 0

Query: 4   FCSGGSLVAYADASPINVAPSTSIASFKADVDEKKKKVVVLGTGWAGTSFLKNIKDASYE 63
           F  GG ++ Y+D++P+                 +KKKVVVLG+GW+G SFL  + + +Y+
Sbjct: 29  FSGGGGVLVYSDSNPLKRILHADATLDSDGNPIRKKKVVVLGSGWSGYSFLSYLNNPNYD 88

Query: 64  IQVISPRNYFAFTPLLPSVTCGTVEARSIVEPIRNIVRKKGVDIIFNEAECYKIDAENRK 123
           +QV+SPRN+F FTPLLPSVT GTVEARSIVEPIR ++RKKG +  + EAEC KIDA N+K
Sbjct: 89  VQVVSPRNFFLFTPLLPSVTNGTVEARSIVEPIRGLMRKKGFE--YKEAECVKIDASNKK 148

Query: 124 LYCRSNANNNLNGKKEFVVDYDYLVIAVGAQVNTFNTPGVVENCHFLKEVEDAQRIRRTV 183
           ++CRS   ++L G  EF +DYD L++AVGA+ NTFNTPGV E+ +FLKE EDA  IR +V
Sbjct: 149 IHCRSKEGSSLKGTTEFDMDYDILILAVGAKPNTFNTPGVEEHAYFLKEAEDALNIRHSV 208

Query: 184 IDCFERASLPTLNEEDRKRILHFAVVGGGPTGVEFAAELHDFVNEDLVKLYPGLKDFVKI 243
           IDCFERASLP L EE+RK+ILHF VVGGGPTGVEF+AELHDF+ +D+ K+YP +++F KI
Sbjct: 209 IDCFERASLPNLTEEERKKILHFVVVGGGPTGVEFSAELHDFLVQDVAKIYPKVQEFTKI 268

Query: 244 TLLEAGDHILNMFDKRITTFAEEKFRRDGIDVKTGSIVIKVTDKEISTKEMKSGEVSSMP 303
           TLLEAGDHILNMFDKRIT FAEEKF+RDGID+KTGS+V+ VT  EISTKE ++G++ S P
Sbjct: 269 TLLEAGDHILNMFDKRITAFAEEKFQRDGIDLKTGSMVVGVTADEISTKERETGKIVSEP 328

Query: 304 YGMTVWSTGIGTRPIIKDFMTQIGQVNRRALATDEWLRVEGCDNVYAIGDSATINQRRVM 363
           YGM VWSTGIG+RP+IKDFM QIGQ  RR LATDEWLRVEGCD VYA+GD+ATINQRRVM
Sbjct: 329 YGMVVWSTGIGSRPVIKDFMQQIGQGQRRVLATDEWLRVEGCDGVYALGDTATINQRRVM 388

Query: 364 EDIAAIFSKADKDNSGTLTVKEFQEVINDICERYPQVELYLKNKQMHNIVDLLKGSKGDV 423
           EDIAAIF+KADK N+GTL  K+F  V+ DIC+RYPQVELYLK  ++ NI +LLK + G  
Sbjct: 389 EDIAAIFNKADKGNTGTLKKKDFNSVVKDICQRYPQVELYLKKNKLKNIANLLKSANG-- 448

Query: 424 AKESIELNIEEFKLALSQVDSQMKNLPATAQVAAQQGAYLAKCFNRMEECEQNPEGPLRF 483
             E  ++NIE+FK ALS+VDSQMKNLPATAQVA+QQG YLAKCFN+ME+CE+ PEGPLRF
Sbjct: 449 --EDTQVNIEKFKQALSEVDSQMKNLPATAQVASQQGKYLAKCFNKMEKCEKKPEGPLRF 508

Query: 484 RGTGRHRFRPFRYKHLGQFAPLGGEQTAAQLPGDWVSIGHSSQWLWYSVYASKQVSWRTR 543
           RG GRHRF+PFRY+H G FAPLGGEQTAA+LPGDWVSIGHSSQWLWYSVYASK VSWRTR
Sbjct: 509 RGEGRHRFQPFRYRHFGSFAPLGGEQTAAELPGDWVSIGHSSQWLWYSVYASKLVSWRTR 568

Query: 544 ALVVTDWTRRFVFGRDSSRI 564
            LV++DWTRRFVFGRDSS I
Sbjct: 569 MLVISDWTRRFVFGRDSSSI 582

BLAST of MC03g0833 vs. ExPASy Swiss-Prot
Match: M1BYJ7 (External alternative NAD(P)H-ubiquinone oxidoreductase B1, mitochondrial OS=Solanum tuberosum OX=4113 GN=NDB1 PE=3 SV=1)

HSP 1 Score: 781.6 bits (2017), Expect = 6.1e-225
Identity = 373/563 (66.25%), Postives = 462/563 (82.06%), Query Frame = 0

Query: 1   VLFFCSGGSLVAYADASPINVAPSTSIASFKADVDEKKKKVVVLGTGWAGTSFLKNIKDA 60
           VL   S G L+ YA++   NV     +   +   + KKK+VVVLGTGW GTSFLK++  +
Sbjct: 22  VLCSVSTGGLLVYAES---NVESGKQVVE-QNQPESKKKRVVVLGTGWGGTSFLKDVDIS 81

Query: 61  SYEIQVISPRNYFAFTPLLPSVTCGTVEARSIVEPIRNIVRKKGVDIIFNEAECYKIDAE 120
           SY++QV+SPRNYFAFTPLLPSVTCGTVEARSIVEP+RNI++K+  +I F EAEC KID E
Sbjct: 82  SYDVQVVSPRNYFAFTPLLPSVTCGTVEARSIVEPVRNIIKKRSGEIQFWEAECLKIDPE 141

Query: 121 NRKLYCRSNANNNLNGKKEFVVDYDYLVIAVGAQVNTFNTPGVVENCHFLKEVEDAQRIR 180
           NR + CRS  N+NL G+ +F + YDYLV+AVGAQVNTFNTPGV+E+CHFLKEVEDAQRIR
Sbjct: 142 NRTVSCRSGINDNLAGQNDFSLQYDYLVVAVGAQVNTFNTPGVMEHCHFLKEVEDAQRIR 201

Query: 181 RTVIDCFERASLPTLNEEDRKRILHFAVVGGGPTGVEFAAELHDFVNEDLVKLYPGLKDF 240
           RTVIDCFE++ +P L+EE+R+  LHF +VGGGPTGVEFAAELHD+V EDLVK+YP +KDF
Sbjct: 202 RTVIDCFEKSVIPGLSEEERRTNLHFVIVGGGPTGVEFAAELHDYVYEDLVKIYPSVKDF 261

Query: 241 VKITLLEAGDHILNMFDKRITTFAEEKFRRDGIDVKTGSIVIKVTDKEISTKEMKSGEVS 300
           VKIT++++GDHILN FD+RI++FAE+KF+RDGI+V TG  V  V+D  I+ K   +G+  
Sbjct: 262 VKITVIQSGDHILNTFDERISSFAEQKFQRDGIEVSTGCRVTSVSDHFINMKVKSTGKHV 321

Query: 301 SMPYGMTVWSTGIGTRPIIKDFMTQIGQVNRRALATDEWLRVEGCDNVYAIGDSATINQR 360
            +PYGM VWSTG+GTRP +KDFM Q+GQ  RR LATDEWLRV+GC NVYA+GD A+I+QR
Sbjct: 322 EVPYGMVVWSTGVGTRPFVKDFMEQVGQEKRRILATDEWLRVKGCSNVYALGDCASIDQR 381

Query: 361 RVMEDIAAIFSKADKDNSGTLTVKEFQEVINDICERYPQVELYLKNKQMHNIVDLLKGSK 420
           +VMEDI+AIF  ADKD+SGTL+++EF++V+ DI  RYPQV+LYLKNK +    DL + S+
Sbjct: 382 KVMEDISAIFKAADKDDSGTLSIEEFRDVLEDIIIRYPQVDLYLKNKHLLEAKDLFRDSE 441

Query: 421 GDVAKESIELNIEEFKLALSQVDSQMKNLPATAQVAAQQGAYLAKCFNRMEECEQNPEGP 480
           G+   E  E++IE FKLALS VDSQMK+LPATAQVAAQQG YL++C NR ++C+ NPEGP
Sbjct: 442 GN---EREEVDIEGFKLALSHVDSQMKSLPATAQVAAQQGTYLSRCLNRWDQCKSNPEGP 501

Query: 481 LRFRGTGRHRFRPFRYKHLGQFAPLGGEQTAAQLPGDWVSIGHSSQWLWYSVYASKQVSW 540
             F+ +GRH F PF Y+HLGQFAPLGG+Q AA+LPGDWVS+GHS+QWLWYSVYASKQVSW
Sbjct: 502 RHFKSSGRHEFLPFEYRHLGQFAPLGGDQAAAELPGDWVSMGHSTQWLWYSVYASKQVSW 561

Query: 541 RTRALVVTDWTRRFVFGRDSSRI 564
           RTR LVV DW RR++FGRDSSRI
Sbjct: 562 RTRYLVVGDWVRRYIFGRDSSRI 577

BLAST of MC03g0833 vs. ExPASy Swiss-Prot
Match: Q9ST62 (External alternative NAD(P)H-ubiquinone oxidoreductase B1, mitochondrial OS=Solanum tuberosum OX=4113 GN=NDB1 PE=1 SV=1)

HSP 1 Score: 777.7 bits (2007), Expect = 8.9e-224
Identity = 372/563 (66.07%), Postives = 459/563 (81.53%), Query Frame = 0

Query: 1   VLFFCSGGSLVAYADASPINVAPSTSIASFKADVDEKKKKVVVLGTGWAGTSFLKNIKDA 60
           VL   S G L+ YA++   NV     +   +   + KKK+VVVLGTGW GTSFLK++  +
Sbjct: 22  VLCSVSTGGLLVYAES---NVESGKQVVE-QNQPESKKKRVVVLGTGWGGTSFLKDVDIS 81

Query: 61  SYEIQVISPRNYFAFTPLLPSVTCGTVEARSIVEPIRNIVRKKGVDIIFNEAECYKIDAE 120
           SY++QV+SPRNYFAFTPLLPSVTCGTVEARSIVEP+RNI++K+  +I F EAEC KID  
Sbjct: 82  SYDVQVVSPRNYFAFTPLLPSVTCGTVEARSIVEPVRNIIKKRSGEIQFWEAECLKIDPV 141

Query: 121 NRKLYCRSNANNNLNGKKEFVVDYDYLVIAVGAQVNTFNTPGVVENCHFLKEVEDAQRIR 180
           NR + CRS  N+NL G  +F + YDYLV+AVGAQVNTFNTPGV+E+CHFLKEVEDAQRIR
Sbjct: 142 NRTVSCRSGINDNLAGHNDFSLQYDYLVVAVGAQVNTFNTPGVMEHCHFLKEVEDAQRIR 201

Query: 181 RTVIDCFERASLPTLNEEDRKRILHFAVVGGGPTGVEFAAELHDFVNEDLVKLYPGLKDF 240
           RTVIDCFE++ +P L+EE+R+  LHF +VGGGPTGVEFAAELHD+V EDLVK+YP +KDF
Sbjct: 202 RTVIDCFEKSVIPGLSEEERRTNLHFVIVGGGPTGVEFAAELHDYVYEDLVKIYPSVKDF 261

Query: 241 VKITLLEAGDHILNMFDKRITTFAEEKFRRDGIDVKTGSIVIKVTDKEISTKEMKSGEVS 300
           VKIT++++GDHILN FD+RI++FAE+KF+RDGI+V TG  V  V+D  I+ K   +G+  
Sbjct: 262 VKITVIQSGDHILNTFDERISSFAEQKFQRDGIEVSTGCRVTSVSDHFINMKVKSTGKHV 321

Query: 301 SMPYGMTVWSTGIGTRPIIKDFMTQIGQVNRRALATDEWLRVEGCDNVYAIGDSATINQR 360
            +PYGM VWSTG+GTRP +KDFM Q+GQ  RR LATDEWLRV+GC NVYA+GD A+++Q 
Sbjct: 322 EVPYGMVVWSTGVGTRPFVKDFMEQVGQEKRRILATDEWLRVKGCSNVYALGDCASVDQH 381

Query: 361 RVMEDIAAIFSKADKDNSGTLTVKEFQEVINDICERYPQVELYLKNKQMHNIVDLLKGSK 420
           +VMEDI+ IF  ADKD+SGTL+V+EF++V+ DI  RYPQV+LYLKNK +    DL + S+
Sbjct: 382 KVMEDISTIFEAADKDDSGTLSVEEFRDVLEDIIIRYPQVDLYLKNKHLLEAKDLFRDSE 441

Query: 421 GDVAKESIELNIEEFKLALSQVDSQMKNLPATAQVAAQQGAYLAKCFNRMEECEQNPEGP 480
           G+   E  E++IE FKLALS VDSQMK+LPATAQVAAQQG YLA+C NR ++C+ NPEGP
Sbjct: 442 GN---EREEVDIEGFKLALSHVDSQMKSLPATAQVAAQQGTYLARCLNRWDQCKSNPEGP 501

Query: 481 LRFRGTGRHRFRPFRYKHLGQFAPLGGEQTAAQLPGDWVSIGHSSQWLWYSVYASKQVSW 540
            RF+ +GRH F PF Y+HLGQFAPLGG+Q AA+LPGDWVS+GHS+QWLWYSVYASKQVSW
Sbjct: 502 RRFKSSGRHEFLPFEYRHLGQFAPLGGDQAAAELPGDWVSMGHSTQWLWYSVYASKQVSW 561

Query: 541 RTRALVVTDWTRRFVFGRDSSRI 564
           RTR LVV DW RR++FGRDSSRI
Sbjct: 562 RTRYLVVGDWVRRYIFGRDSSRI 577

BLAST of MC03g0833 vs. NCBI nr
Match: XP_022140190.1 (external alternative NAD(P)H-ubiquinone oxidoreductase B3, mitochondrial [Momordica charantia])

HSP 1 Score: 1123 bits (2906), Expect = 0.0
Identity = 560/563 (99.47%), Postives = 560/563 (99.47%), Query Frame = 0

Query: 1   VLFFCSGGSLVAYADASPINVAPSTSIASFKADVDEKKKKVVVLGTGWAGTSFLKNIKDA 60
           VL   SGGSLVAYADASPINVAPSTSIASFKADVDEKKKKVVVLGTGWAGTSFLKNIKDA
Sbjct: 25  VLVTISGGSLVAYADASPINVAPSTSIASFKADVDEKKKKVVVLGTGWAGTSFLKNIKDA 84

Query: 61  SYEIQVISPRNYFAFTPLLPSVTCGTVEARSIVEPIRNIVRKKGVDIIFNEAECYKIDAE 120
           SYEIQVISPRNYFAFTPLLPSVTCGTVEARSIVEPIRNIVRKKGVDIIFNEAECYKIDAE
Sbjct: 85  SYEIQVISPRNYFAFTPLLPSVTCGTVEARSIVEPIRNIVRKKGVDIIFNEAECYKIDAE 144

Query: 121 NRKLYCRSNANNNLNGKKEFVVDYDYLVIAVGAQVNTFNTPGVVENCHFLKEVEDAQRIR 180
           NRKLYCRSNANNNLNGKKEFVVDYDYLVIAVGAQVNTFNTPGVVENCHFLKEVEDAQRIR
Sbjct: 145 NRKLYCRSNANNNLNGKKEFVVDYDYLVIAVGAQVNTFNTPGVVENCHFLKEVEDAQRIR 204

Query: 181 RTVIDCFERASLPTLNEEDRKRILHFAVVGGGPTGVEFAAELHDFVNEDLVKLYPGLKDF 240
           RTVIDCFERASLPTLNEEDRKRILHFAVVGGGPTGVEFAAELHDFVNEDLVKLYPGLKDF
Sbjct: 205 RTVIDCFERASLPTLNEEDRKRILHFAVVGGGPTGVEFAAELHDFVNEDLVKLYPGLKDF 264

Query: 241 VKITLLEAGDHILNMFDKRITTFAEEKFRRDGIDVKTGSIVIKVTDKEISTKEMKSGEVS 300
           VKITLLEAGDHILNMFDKRITTFAEEKFRRDGIDVKTGSIVIKVTDKEISTKEMKSGEVS
Sbjct: 265 VKITLLEAGDHILNMFDKRITTFAEEKFRRDGIDVKTGSIVIKVTDKEISTKEMKSGEVS 324

Query: 301 SMPYGMTVWSTGIGTRPIIKDFMTQIGQVNRRALATDEWLRVEGCDNVYAIGDSATINQR 360
           SMPYGMTVWSTGIGTRPIIKDFMTQIGQVNRRALATDEWLRVEGCDNVYAIGDSATINQR
Sbjct: 325 SMPYGMTVWSTGIGTRPIIKDFMTQIGQVNRRALATDEWLRVEGCDNVYAIGDSATINQR 384

Query: 361 RVMEDIAAIFSKADKDNSGTLTVKEFQEVINDICERYPQVELYLKNKQMHNIVDLLKGSK 420
           RVMEDIAAIFSKADKDNSGTLTVKEFQEVINDICERYPQVELYLKNKQMHNIVDLLKGSK
Sbjct: 385 RVMEDIAAIFSKADKDNSGTLTVKEFQEVINDICERYPQVELYLKNKQMHNIVDLLKGSK 444

Query: 421 GDVAKESIELNIEEFKLALSQVDSQMKNLPATAQVAAQQGAYLAKCFNRMEECEQNPEGP 480
           GDVAKESIELNIEEFKLALSQVDSQMKNLPATAQVAAQQGAYLAKCFNRMEECEQNPEGP
Sbjct: 445 GDVAKESIELNIEEFKLALSQVDSQMKNLPATAQVAAQQGAYLAKCFNRMEECEQNPEGP 504

Query: 481 LRFRGTGRHRFRPFRYKHLGQFAPLGGEQTAAQLPGDWVSIGHSSQWLWYSVYASKQVSW 540
           LRFRGTGRHRFRPFRYKHLGQFAPLGGEQTAAQLPGDWVSIGHSSQWLWYSVYASKQVSW
Sbjct: 505 LRFRGTGRHRFRPFRYKHLGQFAPLGGEQTAAQLPGDWVSIGHSSQWLWYSVYASKQVSW 564

Query: 541 RTRALVVTDWTRRFVFGRDSSRI 563
           RTRALVVTDWTRRFVFGRDSSRI
Sbjct: 565 RTRALVVTDWTRRFVFGRDSSRI 587

BLAST of MC03g0833 vs. NCBI nr
Match: XP_004146581.1 (external alternative NAD(P)H-ubiquinone oxidoreductase B2, mitochondrial [Cucumis sativus] >KGN64627.1 hypothetical protein Csa_013741 [Cucumis sativus])

HSP 1 Score: 1051 bits (2719), Expect = 0.0
Identity = 523/563 (92.90%), Postives = 542/563 (96.27%), Query Frame = 0

Query: 1   VLFFCSGGSLVAYADASPINVAPSTSIASFKADVDEKKKKVVVLGTGWAGTSFLKNIKDA 60
           VL   SGGSLVAYADA P N  PS  IAS  A+VDEKKKKVVVLGTGWAGTSFLKNIKD 
Sbjct: 25  VLVTVSGGSLVAYADAGPTNGVPS--IAS-TANVDEKKKKVVVLGTGWAGTSFLKNIKDP 84

Query: 61  SYEIQVISPRNYFAFTPLLPSVTCGTVEARSIVEPIRNIVRKKGVDIIFNEAECYKIDAE 120
           SYE+QVISPRNYFAFTPLLPSVTCGTVEARSIVEPIRN+VRKK VDI FNEAECYKIDAE
Sbjct: 85  SYEVQVISPRNYFAFTPLLPSVTCGTVEARSIVEPIRNLVRKKRVDIRFNEAECYKIDAE 144

Query: 121 NRKLYCRSNANNNLNGKKEFVVDYDYLVIAVGAQVNTFNTPGVVENCHFLKEVEDAQRIR 180
           NRKLYCRSN NNNLNGKKEFVVDYDYLVIAVGAQVNTFNTPGVVENCHFLKEVEDAQRIR
Sbjct: 145 NRKLYCRSNENNNLNGKKEFVVDYDYLVIAVGAQVNTFNTPGVVENCHFLKEVEDAQRIR 204

Query: 181 RTVIDCFERASLPTLNEEDRKRILHFAVVGGGPTGVEFAAELHDFVNEDLVKLYPGLKDF 240
           RTVIDCFERASLPTL+EEDRK+ILHFA+VGGGPTGVEFAAELHDFVNEDLVKLYPGL++F
Sbjct: 205 RTVIDCFERASLPTLDEEDRKKILHFAIVGGGPTGVEFAAELHDFVNEDLVKLYPGLQEF 264

Query: 241 VKITLLEAGDHILNMFDKRITTFAEEKFRRDGIDVKTGSIVIKVTDKEISTKEMKSGEVS 300
           VKITLLEAGDHILNMFDKRITTFAEEKFRRDGIDVKTGS+VIKVTDKEISTKEMK+GE+S
Sbjct: 265 VKITLLEAGDHILNMFDKRITTFAEEKFRRDGIDVKTGSMVIKVTDKEISTKEMKNGEIS 324

Query: 301 SMPYGMTVWSTGIGTRPIIKDFMTQIGQVNRRALATDEWLRVEGCDNVYAIGDSATINQR 360
           SMPYGMTVWSTGIGTRPIIKDFMTQIGQ NRRALATDEWLRVEGCDNVYA+GD ATINQR
Sbjct: 325 SMPYGMTVWSTGIGTRPIIKDFMTQIGQANRRALATDEWLRVEGCDNVYALGDCATINQR 384

Query: 361 RVMEDIAAIFSKADKDNSGTLTVKEFQEVINDICERYPQVELYLKNKQMHNIVDLLKGSK 420
           +VMEDI+AIFSKADKDNSGTLTVKEFQEVI+DICERYPQVELYLKNKQMHNIVDLLKGSK
Sbjct: 385 KVMEDISAIFSKADKDNSGTLTVKEFQEVIDDICERYPQVELYLKNKQMHNIVDLLKGSK 444

Query: 421 GDVAKESIELNIEEFKLALSQVDSQMKNLPATAQVAAQQGAYLAKCFNRMEECEQNPEGP 480
           GDVAKESIEL+IEEFK ALSQVDSQMKNLPATAQVAAQQGAYLA CFNRMEECE+ PEGP
Sbjct: 445 GDVAKESIELDIEEFKSALSQVDSQMKNLPATAQVAAQQGAYLAHCFNRMEECEKYPEGP 504

Query: 481 LRFRGTGRHRFRPFRYKHLGQFAPLGGEQTAAQLPGDWVSIGHSSQWLWYSVYASKQVSW 540
           LRFRG+GRHRFR FRYKHLGQFAPLGGEQTAAQLPGDWVSIGHSSQWLWYSVYASKQVSW
Sbjct: 505 LRFRGSGRHRFRAFRYKHLGQFAPLGGEQTAAQLPGDWVSIGHSSQWLWYSVYASKQVSW 564

Query: 541 RTRALVVTDWTRRFVFGRDSSRI 563
           RTRALVVTDWTRRF+FGRDSSRI
Sbjct: 565 RTRALVVTDWTRRFIFGRDSSRI 584

BLAST of MC03g0833 vs. NCBI nr
Match: KAG7019646.1 (External alternative NAD(P)H-ubiquinone oxidoreductase B3, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1051 bits (2718), Expect = 0.0
Identity = 523/563 (92.90%), Postives = 542/563 (96.27%), Query Frame = 0

Query: 1   VLFFCSGGSLVAYADASPINVAPSTSIASFKADVDEKKKKVVVLGTGWAGTSFLKNIKDA 60
           VL   SGGSLVAYADASP N  PS  IAS KA+VDEKKKKVVVLGTGWAGTSFLKNIKD 
Sbjct: 25  VLVSVSGGSLVAYADASPTNGVPS--IAS-KANVDEKKKKVVVLGTGWAGTSFLKNIKDP 84

Query: 61  SYEIQVISPRNYFAFTPLLPSVTCGTVEARSIVEPIRNIVRKKGVDIIFNEAECYKIDAE 120
           SYE+QVISPRNYFAFTPLLPSVTCGTVEARSIVEPIRNIVRKK VDI FNEAECYKIDA+
Sbjct: 85  SYEVQVISPRNYFAFTPLLPSVTCGTVEARSIVEPIRNIVRKKRVDIRFNEAECYKIDAK 144

Query: 121 NRKLYCRSNANNNLNGKKEFVVDYDYLVIAVGAQVNTFNTPGVVENCHFLKEVEDAQRIR 180
           N+KLYCRSN NNNLNGKKEFVVDYDYLVI+VGAQVNTFNTPGVVENCHFLKEVEDAQRIR
Sbjct: 145 NKKLYCRSNENNNLNGKKEFVVDYDYLVISVGAQVNTFNTPGVVENCHFLKEVEDAQRIR 204

Query: 181 RTVIDCFERASLPTLNEEDRKRILHFAVVGGGPTGVEFAAELHDFVNEDLVKLYPGLKDF 240
           RTVIDCFERASLPTL+EEDRK+ILHFA+VGGGPTGVEFAAELHDFVNEDLVKLYPGL+DF
Sbjct: 205 RTVIDCFERASLPTLDEEDRKKILHFAIVGGGPTGVEFAAELHDFVNEDLVKLYPGLQDF 264

Query: 241 VKITLLEAGDHILNMFDKRITTFAEEKFRRDGIDVKTGSIVIKVTDKEISTKEMKSGEVS 300
           VKITLLEAGDHILNMFDKRIT FAEEKFRRDGIDVKTGS+VIKVTDKEISTKEMK+GE+S
Sbjct: 265 VKITLLEAGDHILNMFDKRITAFAEEKFRRDGIDVKTGSMVIKVTDKEISTKEMKNGEIS 324

Query: 301 SMPYGMTVWSTGIGTRPIIKDFMTQIGQVNRRALATDEWLRVEGCDNVYAIGDSATINQR 360
           SMPYGMTVWSTGIGTRPIIKDFMTQIGQ NRRALATDEWLRVEGCDNVYA+GD ATINQR
Sbjct: 325 SMPYGMTVWSTGIGTRPIIKDFMTQIGQANRRALATDEWLRVEGCDNVYALGDCATINQR 384

Query: 361 RVMEDIAAIFSKADKDNSGTLTVKEFQEVINDICERYPQVELYLKNKQMHNIVDLLKGSK 420
           RVMEDI+AIF KADKDNSGTLTVKEF+EVI+DICERYPQVELYLKNKQMH+IVDLLKGSK
Sbjct: 385 RVMEDISAIFIKADKDNSGTLTVKEFKEVIDDICERYPQVELYLKNKQMHDIVDLLKGSK 444

Query: 421 GDVAKESIELNIEEFKLALSQVDSQMKNLPATAQVAAQQGAYLAKCFNRMEECEQNPEGP 480
           GDVAKESI L+IEEFK ALSQVDSQMKNLPATAQVAAQQGAYLA CFNRMEECE NPEGP
Sbjct: 445 GDVAKESIALDIEEFKSALSQVDSQMKNLPATAQVAAQQGAYLAHCFNRMEECESNPEGP 504

Query: 481 LRFRGTGRHRFRPFRYKHLGQFAPLGGEQTAAQLPGDWVSIGHSSQWLWYSVYASKQVSW 540
           LRFRG+GRHRFRPFRYKHLGQFAPLGGEQTAAQLPGDWVSIGHSSQWLWYSVYASKQVSW
Sbjct: 505 LRFRGSGRHRFRPFRYKHLGQFAPLGGEQTAAQLPGDWVSIGHSSQWLWYSVYASKQVSW 564

Query: 541 RTRALVVTDWTRRFVFGRDSSRI 563
           RTRALVVTDWTRRFVFGRDSSRI
Sbjct: 565 RTRALVVTDWTRRFVFGRDSSRI 584

BLAST of MC03g0833 vs. NCBI nr
Match: XP_022927618.1 (external alternative NAD(P)H-ubiquinone oxidoreductase B2, mitochondrial [Cucurbita moschata])

HSP 1 Score: 1050 bits (2716), Expect = 0.0
Identity = 522/563 (92.72%), Postives = 542/563 (96.27%), Query Frame = 0

Query: 1   VLFFCSGGSLVAYADASPINVAPSTSIASFKADVDEKKKKVVVLGTGWAGTSFLKNIKDA 60
           VL   SGGSLVAYADASP N  PS  +AS KA+VDEKKKKVVVLGTGWAGTSFLKNIKD 
Sbjct: 25  VLVSVSGGSLVAYADASPTNGVPS--LAS-KANVDEKKKKVVVLGTGWAGTSFLKNIKDP 84

Query: 61  SYEIQVISPRNYFAFTPLLPSVTCGTVEARSIVEPIRNIVRKKGVDIIFNEAECYKIDAE 120
           SYE+QVISPRNYFAFTPLLPSVTCGTVEARSIVEPIRNIVRKK VDI FNEAECYKIDA+
Sbjct: 85  SYEVQVISPRNYFAFTPLLPSVTCGTVEARSIVEPIRNIVRKKRVDIRFNEAECYKIDAK 144

Query: 121 NRKLYCRSNANNNLNGKKEFVVDYDYLVIAVGAQVNTFNTPGVVENCHFLKEVEDAQRIR 180
           N+KLYCRSN NNNLNGKKEFVVDYDYLVI+VGAQVNTFNTPGVVENCHFLKEVEDAQRIR
Sbjct: 145 NKKLYCRSNENNNLNGKKEFVVDYDYLVISVGAQVNTFNTPGVVENCHFLKEVEDAQRIR 204

Query: 181 RTVIDCFERASLPTLNEEDRKRILHFAVVGGGPTGVEFAAELHDFVNEDLVKLYPGLKDF 240
           RTVIDCFERASLPTL+EEDRK+ILHFA+VGGGPTGVEFAAELHDFVNEDLVKLYPGL+DF
Sbjct: 205 RTVIDCFERASLPTLDEEDRKKILHFAIVGGGPTGVEFAAELHDFVNEDLVKLYPGLQDF 264

Query: 241 VKITLLEAGDHILNMFDKRITTFAEEKFRRDGIDVKTGSIVIKVTDKEISTKEMKSGEVS 300
           VKITLLEAGDHILNMFDKRIT FAEEKFRRDGIDVKTGS+VIKVTDKEISTKEMK+GE+S
Sbjct: 265 VKITLLEAGDHILNMFDKRITAFAEEKFRRDGIDVKTGSMVIKVTDKEISTKEMKNGEIS 324

Query: 301 SMPYGMTVWSTGIGTRPIIKDFMTQIGQVNRRALATDEWLRVEGCDNVYAIGDSATINQR 360
           SMPYGMTVWSTGIGTRPIIKDFMTQIGQ NRRALATDEWLRVEGCDNVYA+GD ATINQR
Sbjct: 325 SMPYGMTVWSTGIGTRPIIKDFMTQIGQANRRALATDEWLRVEGCDNVYALGDCATINQR 384

Query: 361 RVMEDIAAIFSKADKDNSGTLTVKEFQEVINDICERYPQVELYLKNKQMHNIVDLLKGSK 420
           RVMEDI+AIF KADKDNSGTLTVKEF+EVI+DICERYPQVELYLKNKQMH+IVDLLKGSK
Sbjct: 385 RVMEDISAIFIKADKDNSGTLTVKEFKEVIDDICERYPQVELYLKNKQMHDIVDLLKGSK 444

Query: 421 GDVAKESIELNIEEFKLALSQVDSQMKNLPATAQVAAQQGAYLAKCFNRMEECEQNPEGP 480
           GDVAKESI L+IEEFK ALSQVDSQMKNLPATAQVAAQQGAYLA CFNRMEECE NPEGP
Sbjct: 445 GDVAKESIALDIEEFKSALSQVDSQMKNLPATAQVAAQQGAYLAHCFNRMEECESNPEGP 504

Query: 481 LRFRGTGRHRFRPFRYKHLGQFAPLGGEQTAAQLPGDWVSIGHSSQWLWYSVYASKQVSW 540
           LRFRG+GRHRFRPFRYKHLGQFAPLGGEQTAAQLPGDWVSIGHSSQWLWYSVYASKQVSW
Sbjct: 505 LRFRGSGRHRFRPFRYKHLGQFAPLGGEQTAAQLPGDWVSIGHSSQWLWYSVYASKQVSW 564

Query: 541 RTRALVVTDWTRRFVFGRDSSRI 563
           RTRALVVTDWTRRFVFGRDSSRI
Sbjct: 565 RTRALVVTDWTRRFVFGRDSSRI 584

BLAST of MC03g0833 vs. NCBI nr
Match: XP_008441960.1 (PREDICTED: external alternative NAD(P)H-ubiquinone oxidoreductase B2, mitochondrial [Cucumis melo])

HSP 1 Score: 1050 bits (2715), Expect = 0.0
Identity = 522/563 (92.72%), Postives = 542/563 (96.27%), Query Frame = 0

Query: 1   VLFFCSGGSLVAYADASPINVAPSTSIASFKADVDEKKKKVVVLGTGWAGTSFLKNIKDA 60
           V+   SGGSLVAYADA P N  PS  IAS  A++DEKKKKVVVLGTGWAGTSFLKNIKD 
Sbjct: 25  VIVTVSGGSLVAYADAGPTNGVPS--IAS-TANLDEKKKKVVVLGTGWAGTSFLKNIKDP 84

Query: 61  SYEIQVISPRNYFAFTPLLPSVTCGTVEARSIVEPIRNIVRKKGVDIIFNEAECYKIDAE 120
           SYE+QVISPRNYFAFTPLLPSVTCGTVEARSIVEPIRN+VRKK VDI FNEAECYKIDAE
Sbjct: 85  SYEVQVISPRNYFAFTPLLPSVTCGTVEARSIVEPIRNLVRKKRVDIRFNEAECYKIDAE 144

Query: 121 NRKLYCRSNANNNLNGKKEFVVDYDYLVIAVGAQVNTFNTPGVVENCHFLKEVEDAQRIR 180
           NRKLYCRSN NNNLNGKKEFVVDYDYLVIAVGAQVNTFNTPGVVENCHFLKEVEDAQRIR
Sbjct: 145 NRKLYCRSNENNNLNGKKEFVVDYDYLVIAVGAQVNTFNTPGVVENCHFLKEVEDAQRIR 204

Query: 181 RTVIDCFERASLPTLNEEDRKRILHFAVVGGGPTGVEFAAELHDFVNEDLVKLYPGLKDF 240
           RTVIDCFERASLPTL+EEDRK+ILHFAVVGGGPTGVEFAAELHDFVNEDLVKLYPGL++F
Sbjct: 205 RTVIDCFERASLPTLDEEDRKKILHFAVVGGGPTGVEFAAELHDFVNEDLVKLYPGLQEF 264

Query: 241 VKITLLEAGDHILNMFDKRITTFAEEKFRRDGIDVKTGSIVIKVTDKEISTKEMKSGEVS 300
           VKITLLEAGDHILNMFDKRITTFAEEKFRRDGIDVKTGS+VIKVTDKEISTKEMK+GE+S
Sbjct: 265 VKITLLEAGDHILNMFDKRITTFAEEKFRRDGIDVKTGSMVIKVTDKEISTKEMKNGEIS 324

Query: 301 SMPYGMTVWSTGIGTRPIIKDFMTQIGQVNRRALATDEWLRVEGCDNVYAIGDSATINQR 360
           SMPYGMTVWSTGIGTRPIIKDFMTQIGQ NRRALATDEWLRVEGCDNVYA+GD ATINQR
Sbjct: 325 SMPYGMTVWSTGIGTRPIIKDFMTQIGQANRRALATDEWLRVEGCDNVYALGDCATINQR 384

Query: 361 RVMEDIAAIFSKADKDNSGTLTVKEFQEVINDICERYPQVELYLKNKQMHNIVDLLKGSK 420
           +VMEDI+AIFSKADKDNSGTLTVKEFQEVI+DICERYPQVELYLKNKQMHNIVDLLKGSK
Sbjct: 385 KVMEDISAIFSKADKDNSGTLTVKEFQEVIDDICERYPQVELYLKNKQMHNIVDLLKGSK 444

Query: 421 GDVAKESIELNIEEFKLALSQVDSQMKNLPATAQVAAQQGAYLAKCFNRMEECEQNPEGP 480
            DVAKESIEL+IEEFK ALSQVDSQMKNLPATAQVAAQQGAYLA CFNRMEECE+ PEGP
Sbjct: 445 EDVAKESIELDIEEFKSALSQVDSQMKNLPATAQVAAQQGAYLAHCFNRMEECEKYPEGP 504

Query: 481 LRFRGTGRHRFRPFRYKHLGQFAPLGGEQTAAQLPGDWVSIGHSSQWLWYSVYASKQVSW 540
           LRFRG+GRHRFRPFRYKHLGQFAPLGGEQTAAQLPGDWVSIGHSSQWLWYSVYASKQVSW
Sbjct: 505 LRFRGSGRHRFRPFRYKHLGQFAPLGGEQTAAQLPGDWVSIGHSSQWLWYSVYASKQVSW 564

Query: 541 RTRALVVTDWTRRFVFGRDSSRI 563
           RTRALVVTDWTRRF+FGRDSSRI
Sbjct: 565 RTRALVVTDWTRRFIFGRDSSRI 584

BLAST of MC03g0833 vs. ExPASy TrEMBL
Match: A0A6J1CEF1 (NADH:ubiquinone reductase (non-electrogenic) OS=Momordica charantia OX=3673 GN=LOC111010919 PE=3 SV=1)

HSP 1 Score: 1123 bits (2906), Expect = 0.0
Identity = 560/563 (99.47%), Postives = 560/563 (99.47%), Query Frame = 0

Query: 1   VLFFCSGGSLVAYADASPINVAPSTSIASFKADVDEKKKKVVVLGTGWAGTSFLKNIKDA 60
           VL   SGGSLVAYADASPINVAPSTSIASFKADVDEKKKKVVVLGTGWAGTSFLKNIKDA
Sbjct: 25  VLVTISGGSLVAYADASPINVAPSTSIASFKADVDEKKKKVVVLGTGWAGTSFLKNIKDA 84

Query: 61  SYEIQVISPRNYFAFTPLLPSVTCGTVEARSIVEPIRNIVRKKGVDIIFNEAECYKIDAE 120
           SYEIQVISPRNYFAFTPLLPSVTCGTVEARSIVEPIRNIVRKKGVDIIFNEAECYKIDAE
Sbjct: 85  SYEIQVISPRNYFAFTPLLPSVTCGTVEARSIVEPIRNIVRKKGVDIIFNEAECYKIDAE 144

Query: 121 NRKLYCRSNANNNLNGKKEFVVDYDYLVIAVGAQVNTFNTPGVVENCHFLKEVEDAQRIR 180
           NRKLYCRSNANNNLNGKKEFVVDYDYLVIAVGAQVNTFNTPGVVENCHFLKEVEDAQRIR
Sbjct: 145 NRKLYCRSNANNNLNGKKEFVVDYDYLVIAVGAQVNTFNTPGVVENCHFLKEVEDAQRIR 204

Query: 181 RTVIDCFERASLPTLNEEDRKRILHFAVVGGGPTGVEFAAELHDFVNEDLVKLYPGLKDF 240
           RTVIDCFERASLPTLNEEDRKRILHFAVVGGGPTGVEFAAELHDFVNEDLVKLYPGLKDF
Sbjct: 205 RTVIDCFERASLPTLNEEDRKRILHFAVVGGGPTGVEFAAELHDFVNEDLVKLYPGLKDF 264

Query: 241 VKITLLEAGDHILNMFDKRITTFAEEKFRRDGIDVKTGSIVIKVTDKEISTKEMKSGEVS 300
           VKITLLEAGDHILNMFDKRITTFAEEKFRRDGIDVKTGSIVIKVTDKEISTKEMKSGEVS
Sbjct: 265 VKITLLEAGDHILNMFDKRITTFAEEKFRRDGIDVKTGSIVIKVTDKEISTKEMKSGEVS 324

Query: 301 SMPYGMTVWSTGIGTRPIIKDFMTQIGQVNRRALATDEWLRVEGCDNVYAIGDSATINQR 360
           SMPYGMTVWSTGIGTRPIIKDFMTQIGQVNRRALATDEWLRVEGCDNVYAIGDSATINQR
Sbjct: 325 SMPYGMTVWSTGIGTRPIIKDFMTQIGQVNRRALATDEWLRVEGCDNVYAIGDSATINQR 384

Query: 361 RVMEDIAAIFSKADKDNSGTLTVKEFQEVINDICERYPQVELYLKNKQMHNIVDLLKGSK 420
           RVMEDIAAIFSKADKDNSGTLTVKEFQEVINDICERYPQVELYLKNKQMHNIVDLLKGSK
Sbjct: 385 RVMEDIAAIFSKADKDNSGTLTVKEFQEVINDICERYPQVELYLKNKQMHNIVDLLKGSK 444

Query: 421 GDVAKESIELNIEEFKLALSQVDSQMKNLPATAQVAAQQGAYLAKCFNRMEECEQNPEGP 480
           GDVAKESIELNIEEFKLALSQVDSQMKNLPATAQVAAQQGAYLAKCFNRMEECEQNPEGP
Sbjct: 445 GDVAKESIELNIEEFKLALSQVDSQMKNLPATAQVAAQQGAYLAKCFNRMEECEQNPEGP 504

Query: 481 LRFRGTGRHRFRPFRYKHLGQFAPLGGEQTAAQLPGDWVSIGHSSQWLWYSVYASKQVSW 540
           LRFRGTGRHRFRPFRYKHLGQFAPLGGEQTAAQLPGDWVSIGHSSQWLWYSVYASKQVSW
Sbjct: 505 LRFRGTGRHRFRPFRYKHLGQFAPLGGEQTAAQLPGDWVSIGHSSQWLWYSVYASKQVSW 564

Query: 541 RTRALVVTDWTRRFVFGRDSSRI 563
           RTRALVVTDWTRRFVFGRDSSRI
Sbjct: 565 RTRALVVTDWTRRFVFGRDSSRI 587

BLAST of MC03g0833 vs. ExPASy TrEMBL
Match: A0A0A0LV07 (NADH:ubiquinone reductase (non-electrogenic) OS=Cucumis sativus OX=3659 GN=Csa_1G071890 PE=3 SV=1)

HSP 1 Score: 1051 bits (2719), Expect = 0.0
Identity = 523/563 (92.90%), Postives = 542/563 (96.27%), Query Frame = 0

Query: 1   VLFFCSGGSLVAYADASPINVAPSTSIASFKADVDEKKKKVVVLGTGWAGTSFLKNIKDA 60
           VL   SGGSLVAYADA P N  PS  IAS  A+VDEKKKKVVVLGTGWAGTSFLKNIKD 
Sbjct: 25  VLVTVSGGSLVAYADAGPTNGVPS--IAS-TANVDEKKKKVVVLGTGWAGTSFLKNIKDP 84

Query: 61  SYEIQVISPRNYFAFTPLLPSVTCGTVEARSIVEPIRNIVRKKGVDIIFNEAECYKIDAE 120
           SYE+QVISPRNYFAFTPLLPSVTCGTVEARSIVEPIRN+VRKK VDI FNEAECYKIDAE
Sbjct: 85  SYEVQVISPRNYFAFTPLLPSVTCGTVEARSIVEPIRNLVRKKRVDIRFNEAECYKIDAE 144

Query: 121 NRKLYCRSNANNNLNGKKEFVVDYDYLVIAVGAQVNTFNTPGVVENCHFLKEVEDAQRIR 180
           NRKLYCRSN NNNLNGKKEFVVDYDYLVIAVGAQVNTFNTPGVVENCHFLKEVEDAQRIR
Sbjct: 145 NRKLYCRSNENNNLNGKKEFVVDYDYLVIAVGAQVNTFNTPGVVENCHFLKEVEDAQRIR 204

Query: 181 RTVIDCFERASLPTLNEEDRKRILHFAVVGGGPTGVEFAAELHDFVNEDLVKLYPGLKDF 240
           RTVIDCFERASLPTL+EEDRK+ILHFA+VGGGPTGVEFAAELHDFVNEDLVKLYPGL++F
Sbjct: 205 RTVIDCFERASLPTLDEEDRKKILHFAIVGGGPTGVEFAAELHDFVNEDLVKLYPGLQEF 264

Query: 241 VKITLLEAGDHILNMFDKRITTFAEEKFRRDGIDVKTGSIVIKVTDKEISTKEMKSGEVS 300
           VKITLLEAGDHILNMFDKRITTFAEEKFRRDGIDVKTGS+VIKVTDKEISTKEMK+GE+S
Sbjct: 265 VKITLLEAGDHILNMFDKRITTFAEEKFRRDGIDVKTGSMVIKVTDKEISTKEMKNGEIS 324

Query: 301 SMPYGMTVWSTGIGTRPIIKDFMTQIGQVNRRALATDEWLRVEGCDNVYAIGDSATINQR 360
           SMPYGMTVWSTGIGTRPIIKDFMTQIGQ NRRALATDEWLRVEGCDNVYA+GD ATINQR
Sbjct: 325 SMPYGMTVWSTGIGTRPIIKDFMTQIGQANRRALATDEWLRVEGCDNVYALGDCATINQR 384

Query: 361 RVMEDIAAIFSKADKDNSGTLTVKEFQEVINDICERYPQVELYLKNKQMHNIVDLLKGSK 420
           +VMEDI+AIFSKADKDNSGTLTVKEFQEVI+DICERYPQVELYLKNKQMHNIVDLLKGSK
Sbjct: 385 KVMEDISAIFSKADKDNSGTLTVKEFQEVIDDICERYPQVELYLKNKQMHNIVDLLKGSK 444

Query: 421 GDVAKESIELNIEEFKLALSQVDSQMKNLPATAQVAAQQGAYLAKCFNRMEECEQNPEGP 480
           GDVAKESIEL+IEEFK ALSQVDSQMKNLPATAQVAAQQGAYLA CFNRMEECE+ PEGP
Sbjct: 445 GDVAKESIELDIEEFKSALSQVDSQMKNLPATAQVAAQQGAYLAHCFNRMEECEKYPEGP 504

Query: 481 LRFRGTGRHRFRPFRYKHLGQFAPLGGEQTAAQLPGDWVSIGHSSQWLWYSVYASKQVSW 540
           LRFRG+GRHRFR FRYKHLGQFAPLGGEQTAAQLPGDWVSIGHSSQWLWYSVYASKQVSW
Sbjct: 505 LRFRGSGRHRFRAFRYKHLGQFAPLGGEQTAAQLPGDWVSIGHSSQWLWYSVYASKQVSW 564

Query: 541 RTRALVVTDWTRRFVFGRDSSRI 563
           RTRALVVTDWTRRF+FGRDSSRI
Sbjct: 565 RTRALVVTDWTRRFIFGRDSSRI 584

BLAST of MC03g0833 vs. ExPASy TrEMBL
Match: A0A6J1ELH7 (NADH:ubiquinone reductase (non-electrogenic) OS=Cucurbita moschata OX=3662 GN=LOC111434385 PE=3 SV=1)

HSP 1 Score: 1050 bits (2716), Expect = 0.0
Identity = 522/563 (92.72%), Postives = 542/563 (96.27%), Query Frame = 0

Query: 1   VLFFCSGGSLVAYADASPINVAPSTSIASFKADVDEKKKKVVVLGTGWAGTSFLKNIKDA 60
           VL   SGGSLVAYADASP N  PS  +AS KA+VDEKKKKVVVLGTGWAGTSFLKNIKD 
Sbjct: 25  VLVSVSGGSLVAYADASPTNGVPS--LAS-KANVDEKKKKVVVLGTGWAGTSFLKNIKDP 84

Query: 61  SYEIQVISPRNYFAFTPLLPSVTCGTVEARSIVEPIRNIVRKKGVDIIFNEAECYKIDAE 120
           SYE+QVISPRNYFAFTPLLPSVTCGTVEARSIVEPIRNIVRKK VDI FNEAECYKIDA+
Sbjct: 85  SYEVQVISPRNYFAFTPLLPSVTCGTVEARSIVEPIRNIVRKKRVDIRFNEAECYKIDAK 144

Query: 121 NRKLYCRSNANNNLNGKKEFVVDYDYLVIAVGAQVNTFNTPGVVENCHFLKEVEDAQRIR 180
           N+KLYCRSN NNNLNGKKEFVVDYDYLVI+VGAQVNTFNTPGVVENCHFLKEVEDAQRIR
Sbjct: 145 NKKLYCRSNENNNLNGKKEFVVDYDYLVISVGAQVNTFNTPGVVENCHFLKEVEDAQRIR 204

Query: 181 RTVIDCFERASLPTLNEEDRKRILHFAVVGGGPTGVEFAAELHDFVNEDLVKLYPGLKDF 240
           RTVIDCFERASLPTL+EEDRK+ILHFA+VGGGPTGVEFAAELHDFVNEDLVKLYPGL+DF
Sbjct: 205 RTVIDCFERASLPTLDEEDRKKILHFAIVGGGPTGVEFAAELHDFVNEDLVKLYPGLQDF 264

Query: 241 VKITLLEAGDHILNMFDKRITTFAEEKFRRDGIDVKTGSIVIKVTDKEISTKEMKSGEVS 300
           VKITLLEAGDHILNMFDKRIT FAEEKFRRDGIDVKTGS+VIKVTDKEISTKEMK+GE+S
Sbjct: 265 VKITLLEAGDHILNMFDKRITAFAEEKFRRDGIDVKTGSMVIKVTDKEISTKEMKNGEIS 324

Query: 301 SMPYGMTVWSTGIGTRPIIKDFMTQIGQVNRRALATDEWLRVEGCDNVYAIGDSATINQR 360
           SMPYGMTVWSTGIGTRPIIKDFMTQIGQ NRRALATDEWLRVEGCDNVYA+GD ATINQR
Sbjct: 325 SMPYGMTVWSTGIGTRPIIKDFMTQIGQANRRALATDEWLRVEGCDNVYALGDCATINQR 384

Query: 361 RVMEDIAAIFSKADKDNSGTLTVKEFQEVINDICERYPQVELYLKNKQMHNIVDLLKGSK 420
           RVMEDI+AIF KADKDNSGTLTVKEF+EVI+DICERYPQVELYLKNKQMH+IVDLLKGSK
Sbjct: 385 RVMEDISAIFIKADKDNSGTLTVKEFKEVIDDICERYPQVELYLKNKQMHDIVDLLKGSK 444

Query: 421 GDVAKESIELNIEEFKLALSQVDSQMKNLPATAQVAAQQGAYLAKCFNRMEECEQNPEGP 480
           GDVAKESI L+IEEFK ALSQVDSQMKNLPATAQVAAQQGAYLA CFNRMEECE NPEGP
Sbjct: 445 GDVAKESIALDIEEFKSALSQVDSQMKNLPATAQVAAQQGAYLAHCFNRMEECESNPEGP 504

Query: 481 LRFRGTGRHRFRPFRYKHLGQFAPLGGEQTAAQLPGDWVSIGHSSQWLWYSVYASKQVSW 540
           LRFRG+GRHRFRPFRYKHLGQFAPLGGEQTAAQLPGDWVSIGHSSQWLWYSVYASKQVSW
Sbjct: 505 LRFRGSGRHRFRPFRYKHLGQFAPLGGEQTAAQLPGDWVSIGHSSQWLWYSVYASKQVSW 564

Query: 541 RTRALVVTDWTRRFVFGRDSSRI 563
           RTRALVVTDWTRRFVFGRDSSRI
Sbjct: 565 RTRALVVTDWTRRFVFGRDSSRI 584

BLAST of MC03g0833 vs. ExPASy TrEMBL
Match: A0A6J1KKX9 (NADH:ubiquinone reductase (non-electrogenic) OS=Cucurbita maxima OX=3661 GN=LOC111495131 PE=3 SV=1)

HSP 1 Score: 1050 bits (2715), Expect = 0.0
Identity = 522/563 (92.72%), Postives = 541/563 (96.09%), Query Frame = 0

Query: 1   VLFFCSGGSLVAYADASPINVAPSTSIASFKADVDEKKKKVVVLGTGWAGTSFLKNIKDA 60
           VL   SGGSLVAYADASP N  PS  IAS KA+VDEKKKKVVVLGTGWAGTSFLKN KD 
Sbjct: 25  VLVSVSGGSLVAYADASPTNGVPS--IAS-KANVDEKKKKVVVLGTGWAGTSFLKNFKDP 84

Query: 61  SYEIQVISPRNYFAFTPLLPSVTCGTVEARSIVEPIRNIVRKKGVDIIFNEAECYKIDAE 120
           SYE+QVISPRNYFAFTPLLPSVTCGTVEARSIVEPIRNIVRKK VDI FNEAECYKIDAE
Sbjct: 85  SYEVQVISPRNYFAFTPLLPSVTCGTVEARSIVEPIRNIVRKKRVDIRFNEAECYKIDAE 144

Query: 121 NRKLYCRSNANNNLNGKKEFVVDYDYLVIAVGAQVNTFNTPGVVENCHFLKEVEDAQRIR 180
           N+KLYCRSN NNNLNGKKEFVVDYDYLVI+VGAQVNTFNTPGVVENCHFLKEVEDAQRIR
Sbjct: 145 NKKLYCRSNENNNLNGKKEFVVDYDYLVISVGAQVNTFNTPGVVENCHFLKEVEDAQRIR 204

Query: 181 RTVIDCFERASLPTLNEEDRKRILHFAVVGGGPTGVEFAAELHDFVNEDLVKLYPGLKDF 240
           RTVIDCFERASLPTL+EEDRK+ILHFA+VGGGPTGVEFAAELHDFVNEDLVKLYPGL+DF
Sbjct: 205 RTVIDCFERASLPTLDEEDRKKILHFAIVGGGPTGVEFAAELHDFVNEDLVKLYPGLQDF 264

Query: 241 VKITLLEAGDHILNMFDKRITTFAEEKFRRDGIDVKTGSIVIKVTDKEISTKEMKSGEVS 300
           V+ITLLEAGDHILNMFDKRIT FAEEKFRRDGIDVKTGS+VIKVTDKEISTKEMK+GE+S
Sbjct: 265 VRITLLEAGDHILNMFDKRITAFAEEKFRRDGIDVKTGSMVIKVTDKEISTKEMKNGEIS 324

Query: 301 SMPYGMTVWSTGIGTRPIIKDFMTQIGQVNRRALATDEWLRVEGCDNVYAIGDSATINQR 360
           SMPYGMTVWSTGIGTRPIIKDFMTQIGQ NRRALATDEWLRVEGCDNVYA+GD ATINQR
Sbjct: 325 SMPYGMTVWSTGIGTRPIIKDFMTQIGQANRRALATDEWLRVEGCDNVYALGDCATINQR 384

Query: 361 RVMEDIAAIFSKADKDNSGTLTVKEFQEVINDICERYPQVELYLKNKQMHNIVDLLKGSK 420
           RVMEDI+AIF KADKDNSGTLTVKEF+EVI+DICERYPQVELYLKNKQMH+IVDLLKGSK
Sbjct: 385 RVMEDISAIFIKADKDNSGTLTVKEFKEVIDDICERYPQVELYLKNKQMHDIVDLLKGSK 444

Query: 421 GDVAKESIELNIEEFKLALSQVDSQMKNLPATAQVAAQQGAYLAKCFNRMEECEQNPEGP 480
           GDVAKESI L+IEEFK ALSQVDSQMKNLPATAQVAAQQGAYLA CFNRMEECE NPEGP
Sbjct: 445 GDVAKESIALDIEEFKSALSQVDSQMKNLPATAQVAAQQGAYLAHCFNRMEECESNPEGP 504

Query: 481 LRFRGTGRHRFRPFRYKHLGQFAPLGGEQTAAQLPGDWVSIGHSSQWLWYSVYASKQVSW 540
           LRFRG+GRHRFRPFRYKHLGQFAPLGGEQTAAQLPGDWVSIGHSSQWLWYSVYASKQVSW
Sbjct: 505 LRFRGSGRHRFRPFRYKHLGQFAPLGGEQTAAQLPGDWVSIGHSSQWLWYSVYASKQVSW 564

Query: 541 RTRALVVTDWTRRFVFGRDSSRI 563
           RTRALVVTDWTRRFVFGRDSSRI
Sbjct: 565 RTRALVVTDWTRRFVFGRDSSRI 584

BLAST of MC03g0833 vs. ExPASy TrEMBL
Match: A0A1S3B436 (NADH:ubiquinone reductase (non-electrogenic) OS=Cucumis melo OX=3656 GN=LOC103485958 PE=3 SV=1)

HSP 1 Score: 1050 bits (2715), Expect = 0.0
Identity = 522/563 (92.72%), Postives = 542/563 (96.27%), Query Frame = 0

Query: 1   VLFFCSGGSLVAYADASPINVAPSTSIASFKADVDEKKKKVVVLGTGWAGTSFLKNIKDA 60
           V+   SGGSLVAYADA P N  PS  IAS  A++DEKKKKVVVLGTGWAGTSFLKNIKD 
Sbjct: 25  VIVTVSGGSLVAYADAGPTNGVPS--IAS-TANLDEKKKKVVVLGTGWAGTSFLKNIKDP 84

Query: 61  SYEIQVISPRNYFAFTPLLPSVTCGTVEARSIVEPIRNIVRKKGVDIIFNEAECYKIDAE 120
           SYE+QVISPRNYFAFTPLLPSVTCGTVEARSIVEPIRN+VRKK VDI FNEAECYKIDAE
Sbjct: 85  SYEVQVISPRNYFAFTPLLPSVTCGTVEARSIVEPIRNLVRKKRVDIRFNEAECYKIDAE 144

Query: 121 NRKLYCRSNANNNLNGKKEFVVDYDYLVIAVGAQVNTFNTPGVVENCHFLKEVEDAQRIR 180
           NRKLYCRSN NNNLNGKKEFVVDYDYLVIAVGAQVNTFNTPGVVENCHFLKEVEDAQRIR
Sbjct: 145 NRKLYCRSNENNNLNGKKEFVVDYDYLVIAVGAQVNTFNTPGVVENCHFLKEVEDAQRIR 204

Query: 181 RTVIDCFERASLPTLNEEDRKRILHFAVVGGGPTGVEFAAELHDFVNEDLVKLYPGLKDF 240
           RTVIDCFERASLPTL+EEDRK+ILHFAVVGGGPTGVEFAAELHDFVNEDLVKLYPGL++F
Sbjct: 205 RTVIDCFERASLPTLDEEDRKKILHFAVVGGGPTGVEFAAELHDFVNEDLVKLYPGLQEF 264

Query: 241 VKITLLEAGDHILNMFDKRITTFAEEKFRRDGIDVKTGSIVIKVTDKEISTKEMKSGEVS 300
           VKITLLEAGDHILNMFDKRITTFAEEKFRRDGIDVKTGS+VIKVTDKEISTKEMK+GE+S
Sbjct: 265 VKITLLEAGDHILNMFDKRITTFAEEKFRRDGIDVKTGSMVIKVTDKEISTKEMKNGEIS 324

Query: 301 SMPYGMTVWSTGIGTRPIIKDFMTQIGQVNRRALATDEWLRVEGCDNVYAIGDSATINQR 360
           SMPYGMTVWSTGIGTRPIIKDFMTQIGQ NRRALATDEWLRVEGCDNVYA+GD ATINQR
Sbjct: 325 SMPYGMTVWSTGIGTRPIIKDFMTQIGQANRRALATDEWLRVEGCDNVYALGDCATINQR 384

Query: 361 RVMEDIAAIFSKADKDNSGTLTVKEFQEVINDICERYPQVELYLKNKQMHNIVDLLKGSK 420
           +VMEDI+AIFSKADKDNSGTLTVKEFQEVI+DICERYPQVELYLKNKQMHNIVDLLKGSK
Sbjct: 385 KVMEDISAIFSKADKDNSGTLTVKEFQEVIDDICERYPQVELYLKNKQMHNIVDLLKGSK 444

Query: 421 GDVAKESIELNIEEFKLALSQVDSQMKNLPATAQVAAQQGAYLAKCFNRMEECEQNPEGP 480
            DVAKESIEL+IEEFK ALSQVDSQMKNLPATAQVAAQQGAYLA CFNRMEECE+ PEGP
Sbjct: 445 EDVAKESIELDIEEFKSALSQVDSQMKNLPATAQVAAQQGAYLAHCFNRMEECEKYPEGP 504

Query: 481 LRFRGTGRHRFRPFRYKHLGQFAPLGGEQTAAQLPGDWVSIGHSSQWLWYSVYASKQVSW 540
           LRFRG+GRHRFRPFRYKHLGQFAPLGGEQTAAQLPGDWVSIGHSSQWLWYSVYASKQVSW
Sbjct: 505 LRFRGSGRHRFRPFRYKHLGQFAPLGGEQTAAQLPGDWVSIGHSSQWLWYSVYASKQVSW 564

Query: 541 RTRALVVTDWTRRFVFGRDSSRI 563
           RTRALVVTDWTRRF+FGRDSSRI
Sbjct: 565 RTRALVVTDWTRRFIFGRDSSRI 584

BLAST of MC03g0833 vs. TAIR 10
Match: AT4G21490.1 (NAD(P)H dehydrogenase B3 )

HSP 1 Score: 868.2 bits (2242), Expect = 3.5e-252
Identity = 424/559 (75.85%), Postives = 482/559 (86.23%), Query Frame = 0

Query: 6   SGGSLVAYADASPINVAPSTSIASFKADVDEKKKKVVVLGTGWAGTSFLKNIKDASYEIQ 65
           SGG L+ Y++A+P       S ++   +   +K+KVV+LGTGWAG SFLK + ++SYE+Q
Sbjct: 30  SGGGLIVYSEANP-------SYSNNGVETKTRKRKVVLLGTGWAGASFLKTLNNSSYEVQ 89

Query: 66  VISPRNYFAFTPLLPSVTCGTVEARSIVEPIRNIVRKKGVDIIFNEAECYKIDAENRKLY 125
           VISPRNYFAFTPLLPSVTCGTVEARS+VEPIRNI RK+ V++ F EAEC+KID  ++K+Y
Sbjct: 90  VISPRNYFAFTPLLPSVTCGTVEARSVVEPIRNIARKQNVEMSFLEAECFKIDPGSKKVY 149

Query: 126 CRSNANNNLNGKKEFVVDYDYLVIAVGAQVNTFNTPGVVENCHFLKEVEDAQRIRRTVID 185
           CRS    N  GKKEF VDYDYLVIA GAQ NTFN PGV ENCHFLKEVEDAQRIR TVID
Sbjct: 150 CRSKQGVNSKGKKEFDVDYDYLVIATGAQSNTFNIPGVEENCHFLKEVEDAQRIRSTVID 209

Query: 186 CFERASLPTLNEEDRKRILHFAVVGGGPTGVEFAAELHDFVNEDLVKLYPGLKDFVKITL 245
            FE+ASLP LNE++RKR+LHF VVGGGPTGVEFA+ELHDFVNEDLVKLYP  K+ V+ITL
Sbjct: 210 SFEKASLPGLNEQERKRMLHFVVVGGGPTGVEFASELHDFVNEDLVKLYPKAKNLVQITL 269

Query: 246 LEAGDHILNMFDKRITTFAEEKFRRDGIDVKTGSIVIKVTDKEISTKEMKSGEVSSMPYG 305
           LEA DHIL MFDKRIT FAEEKF RDGIDVK GS+V+KV DKEIS K  K+GEVS++PYG
Sbjct: 270 LEAADHILTMFDKRITEFAEEKFTRDGIDVKLGSMVVKVNDKEISAK-TKAGEVSTIPYG 329

Query: 306 MTVWSTGIGTRPIIKDFMTQIGQVNRRALATDEWLRVEGCDNVYAIGDSATINQRRVMED 365
           M VWSTGIGTRP+IKDFM QIGQ NRRALATDEWLRVEGCDN+YA+GD ATINQR+VMED
Sbjct: 330 MIVWSTGIGTRPVIKDFMKQIGQGNRRALATDEWLRVEGCDNIYALGDCATINQRKVMED 389

Query: 366 IAAIFSKADKDNSGTLTVKEFQEVINDICERYPQVELYLKNKQMHNIVDLLKGSKGD-VA 425
           IAAIF KADK+NSGTLT+KEF EV++DIC+RYPQVELYLK+K MH I DLLK ++ +  +
Sbjct: 390 IAAIFKKADKENSGTLTMKEFHEVMSDICDRYPQVELYLKSKGMHGITDLLKQAQAENGS 449

Query: 426 KESIELNIEEFKLALSQVDSQMKNLPATAQVAAQQGAYLAKCFNRMEECEQNPEGPLRFR 485
            +S+EL+IEE K AL QVDSQ+K LPAT QVAAQQG YLAKCF+RME CE+NPEGP+R R
Sbjct: 450 NKSVELDIEELKSALCQVDSQVKLLPATGQVAAQQGTYLAKCFDRMEVCEKNPEGPIRIR 509

Query: 486 GTGRHRFRPFRYKHLGQFAPLGGEQTAAQLPGDWVSIGHSSQWLWYSVYASKQVSWRTRA 545
           G GRHRFRPFRY+HLGQFAPLGGEQTAAQLPGDWVSIGHSSQWLWYSVYASKQVSWRTR 
Sbjct: 510 GEGRHRFRPFRYRHLGQFAPLGGEQTAAQLPGDWVSIGHSSQWLWYSVYASKQVSWRTRV 569

Query: 546 LVVTDWTRRFVFGRDSSRI 564
           LVV+DW RRF+FGRDSSRI
Sbjct: 570 LVVSDWMRRFIFGRDSSRI 580

BLAST of MC03g0833 vs. TAIR 10
Match: AT4G05020.1 (NAD(P)H dehydrogenase B2 )

HSP 1 Score: 860.9 bits (2223), Expect = 5.7e-250
Identity = 427/559 (76.39%), Postives = 480/559 (85.87%), Query Frame = 0

Query: 6   SGGSLVAYADASPINVAPSTSIASFKADVDEKKKKVVVLGTGWAGTSFLKNIKDASYEIQ 65
           SGG L+AY++A+    A   ++     +   KKKKVV+LGTGWAGTSFLKN+ ++ YE+Q
Sbjct: 30  SGGGLIAYSEANASYGANGGAV----VETGTKKKKVVLLGTGWAGTSFLKNLNNSQYEVQ 89

Query: 66  VISPRNYFAFTPLLPSVTCGTVEARSIVEPIRNIVRKKGVDIIFNEAECYKIDAENRKLY 125
           +ISPRNYFAFTPLLPSVTCGTVEARS+VEPIRNI R K VD  + EAEC+KID  ++K+Y
Sbjct: 90  IISPRNYFAFTPLLPSVTCGTVEARSVVEPIRNIGR-KNVDTSYLEAECFKIDPASKKVY 149

Query: 126 CRSNANNNLNGKKEFVVDYDYLVIAVGAQVNTFNTPGVVENCHFLKEVEDAQRIRRTVID 185
           CRS    + NGKKEF VDYDYLVIA GAQ NTFN PGV ENCHFLKEVEDAQRIR+TVID
Sbjct: 150 CRSKQGLSSNGKKEFSVDYDYLVIATGAQSNTFNIPGVEENCHFLKEVEDAQRIRKTVID 209

Query: 186 CFERASLPTLNEEDRKRILHFAVVGGGPTGVEFAAELHDFVNEDLVKLYPGLKDFVKITL 245
            FE+ASLP L++E+RKRILHF VVGGGPTGVEFAAELHDFV EDLV LYP  K  V+ITL
Sbjct: 210 SFEKASLPELSDEERKRILHFVVVGGGPTGVEFAAELHDFVTEDLVSLYPRAKGSVRITL 269

Query: 246 LEAGDHILNMFDKRITTFAEEKFRRDGIDVKTGSIVIKVTDKEISTKEMKSGEVSSMPYG 305
           LEA DHIL MFDKRIT FAEEKF RDGIDVK GS+V KV +K+IS K  K GEVSS+PYG
Sbjct: 270 LEAADHILTMFDKRITEFAEEKFSRDGIDVKLGSMVTKVNEKDISAK-TKGGEVSSIPYG 329

Query: 306 MTVWSTGIGTRPIIKDFMTQIGQVNRRALATDEWLRVEGCDNVYAIGDSATINQRRVMED 365
           M VWSTGIGTRP+IKDFM QIGQ NRRALATDEWLRVEG DN+YA+GD ATINQR+VMED
Sbjct: 330 MIVWSTGIGTRPVIKDFMKQIGQGNRRALATDEWLRVEGTDNIYALGDCATINQRKVMED 389

Query: 366 IAAIFSKADKDNSGTLTVKEFQEVINDICERYPQVELYLKNKQMHNIVDLLKGSK-GDVA 425
           ++AIFSKADKD SGTLT+KEFQE ++DIC RYPQVELYLK+K+M  I DLLK ++  DV+
Sbjct: 390 VSAIFSKADKDKSGTLTLKEFQEAMDDICVRYPQVELYLKSKRMRGIADLLKEAETDDVS 449

Query: 426 KESIELNIEEFKLALSQVDSQMKNLPATAQVAAQQGAYLAKCFNRMEECEQNPEGPLRFR 485
           K +IEL IEEFK ALSQVDSQ+K LPATAQVAAQQGAYLAKCF+RMEECE++PEGP+R R
Sbjct: 450 KNNIELKIEEFKSALSQVDSQVKFLPATAQVAAQQGAYLAKCFDRMEECEKSPEGPIRMR 509

Query: 486 GTGRHRFRPFRYKHLGQFAPLGGEQTAAQLPGDWVSIGHSSQWLWYSVYASKQVSWRTRA 545
           G GRHRFRPFRY+HLGQFAPLGGEQTAAQLPGDWVSIGHSSQWLWYSVYASKQVSWRTR 
Sbjct: 510 GEGRHRFRPFRYRHLGQFAPLGGEQTAAQLPGDWVSIGHSSQWLWYSVYASKQVSWRTRV 569

Query: 546 LVVTDWTRRFVFGRDSSRI 564
           LVV+DW RRF+FGRDSS I
Sbjct: 570 LVVSDWMRRFIFGRDSSSI 582

BLAST of MC03g0833 vs. TAIR 10
Match: AT4G05020.2 (NAD(P)H dehydrogenase B2 )

HSP 1 Score: 842.4 bits (2175), Expect = 2.1e-244
Identity = 427/596 (71.64%), Postives = 480/596 (80.54%), Query Frame = 0

Query: 6   SGGSLVAYADASPINVAPSTSIASFKADVDEKKKKVVVLGTGWAGTSFLKNIKDASYEIQ 65
           SGG L+AY++A+    A   ++     +   KKKKVV+LGTGWAGTSFLKN+ ++ YE+Q
Sbjct: 30  SGGGLIAYSEANASYGANGGAV----VETGTKKKKVVLLGTGWAGTSFLKNLNNSQYEVQ 89

Query: 66  VISPRNYFAFTPLLPSVTCGTVEARSIVEPIRNIVRKKGVDIIFNEAECYKIDAENRKLY 125
           +ISPRNYFAFTPLLPSVTCGTVEARS+VEPIRNI R K VD  + EAEC+KID  ++K+Y
Sbjct: 90  IISPRNYFAFTPLLPSVTCGTVEARSVVEPIRNIGR-KNVDTSYLEAECFKIDPASKKVY 149

Query: 126 CRSNANNNLNGKKEFVVDYDYLVIAVGAQVNTFNTPGVVENCHFLKEVEDAQRIRRTVID 185
           CRS    + NGKKEF VDYDYLVIA GAQ NTFN PGV ENCHFLKEVEDAQRIR+TVID
Sbjct: 150 CRSKQGLSSNGKKEFSVDYDYLVIATGAQSNTFNIPGVEENCHFLKEVEDAQRIRKTVID 209

Query: 186 CFERASLPTLNEEDRKRILHFAVVGGGPTGVEFAAELHDFVNEDLVKLYPGLKDFVKITL 245
            FE+ASLP L++E+RKRILHF VVGGGPTGVEFAAELHDFV EDLV LYP  K  V+ITL
Sbjct: 210 SFEKASLPELSDEERKRILHFVVVGGGPTGVEFAAELHDFVTEDLVSLYPRAKGSVRITL 269

Query: 246 LEAGDHILNMFDKRITTFAEEKFRRDGIDVKTGSIVIKVTDKEISTKEMKSGEVSSMPYG 305
           LEA DHIL MFDKRIT FAEEKF RDGIDVK GS+V KV +K+IS K  K GEVSS+PYG
Sbjct: 270 LEAADHILTMFDKRITEFAEEKFSRDGIDVKLGSMVTKVNEKDISAK-TKGGEVSSIPYG 329

Query: 306 MTVWSTGIGTRPIIKDFMTQIGQVNRRALATDEWLRVEGCDNVYAIGDSATINQRRVMED 365
           M VWSTGIGTRP+IKDFM QIGQ NRRALATDEWLRVEG DN+YA+GD ATINQR+VMED
Sbjct: 330 MIVWSTGIGTRPVIKDFMKQIGQGNRRALATDEWLRVEGTDNIYALGDCATINQRKVMED 389

Query: 366 IAAIFSKADKDNSGTLTVKEFQEVINDICERYPQVELYLKNKQMHNIVDLLKGSK-GDVA 425
           ++AIFSKADKD SGTLT+KEFQE ++DIC RYPQVELYLK+K+M  I DLLK ++  DV+
Sbjct: 390 VSAIFSKADKDKSGTLTLKEFQEAMDDICVRYPQVELYLKSKRMRGIADLLKEAETDDVS 449

Query: 426 KESIELNIEEFKLALSQVDSQMKNLPATAQVAAQQGAYLAKCFNRMEECEQNPEGPLRFR 485
           K +IEL IEEFK ALSQVDSQ+K LPATAQVAAQQGAYLAKCF+RMEECE++PEGP+R R
Sbjct: 450 KNNIELKIEEFKSALSQVDSQVKFLPATAQVAAQQGAYLAKCFDRMEECEKSPEGPIRMR 509

Query: 486 GTGRHRFRPF-------------------------------------RYKHLGQFAPLGG 545
           G GRHRFRPF                                     RY+HLGQFAPLGG
Sbjct: 510 GEGRHRFRPFRIWKLRGISKTLSQDTTESCALDHKGVGKMELIPYIIRYRHLGQFAPLGG 569

Query: 546 EQTAAQLPGDWVSIGHSSQWLWYSVYASKQVSWRTRALVVTDWTRRFVFGRDSSRI 564
           EQTAAQLPGDWVSIGHSSQWLWYSVYASKQVSWRTR LVV+DW RRF+FGRDSS I
Sbjct: 570 EQTAAQLPGDWVSIGHSSQWLWYSVYASKQVSWRTRVLVVSDWMRRFIFGRDSSSI 619

BLAST of MC03g0833 vs. TAIR 10
Match: AT2G20800.1 (NAD(P)H dehydrogenase B4 )

HSP 1 Score: 807.4 bits (2084), Expect = 7.4e-234
Identity = 387/560 (69.11%), Postives = 463/560 (82.68%), Query Frame = 0

Query: 4   FCSGGSLVAYADASPINVAPSTSIASFKADVDEKKKKVVVLGTGWAGTSFLKNIKDASYE 63
           F  GG ++ Y+D++P+                 +KKKVVVLG+GW+G SFL  + + +Y+
Sbjct: 29  FSGGGGVLVYSDSNPLKRILHADATLDSDGNPIRKKKVVVLGSGWSGYSFLSYLNNPNYD 88

Query: 64  IQVISPRNYFAFTPLLPSVTCGTVEARSIVEPIRNIVRKKGVDIIFNEAECYKIDAENRK 123
           +QV+SPRN+F FTPLLPSVT GTVEARSIVEPIR ++RKKG +  + EAEC KIDA N+K
Sbjct: 89  VQVVSPRNFFLFTPLLPSVTNGTVEARSIVEPIRGLMRKKGFE--YKEAECVKIDASNKK 148

Query: 124 LYCRSNANNNLNGKKEFVVDYDYLVIAVGAQVNTFNTPGVVENCHFLKEVEDAQRIRRTV 183
           ++CRS   ++L G  EF +DYD L++AVGA+ NTFNTPGV E+ +FLKE EDA  IR +V
Sbjct: 149 IHCRSKEGSSLKGTTEFDMDYDILILAVGAKPNTFNTPGVEEHAYFLKEAEDALNIRHSV 208

Query: 184 IDCFERASLPTLNEEDRKRILHFAVVGGGPTGVEFAAELHDFVNEDLVKLYPGLKDFVKI 243
           IDCFERASLP L EE+RK+ILHF VVGGGPTGVEF+AELHDF+ +D+ K+YP +++F KI
Sbjct: 209 IDCFERASLPNLTEEERKKILHFVVVGGGPTGVEFSAELHDFLVQDVAKIYPKVQEFTKI 268

Query: 244 TLLEAGDHILNMFDKRITTFAEEKFRRDGIDVKTGSIVIKVTDKEISTKEMKSGEVSSMP 303
           TLLEAGDHILNMFDKRIT FAEEKF+RDGID+KTGS+V+ VT  EISTKE ++G++ S P
Sbjct: 269 TLLEAGDHILNMFDKRITAFAEEKFQRDGIDLKTGSMVVGVTADEISTKERETGKIVSEP 328

Query: 304 YGMTVWSTGIGTRPIIKDFMTQIGQVNRRALATDEWLRVEGCDNVYAIGDSATINQRRVM 363
           YGM VWSTGIG+RP+IKDFM QIGQ  RR LATDEWLRVEGCD VYA+GD+ATINQRRVM
Sbjct: 329 YGMVVWSTGIGSRPVIKDFMQQIGQGQRRVLATDEWLRVEGCDGVYALGDTATINQRRVM 388

Query: 364 EDIAAIFSKADKDNSGTLTVKEFQEVINDICERYPQVELYLKNKQMHNIVDLLKGSKGDV 423
           EDIAAIF+KADK N+GTL  K+F  V+ DIC+RYPQVELYLK  ++ NI +LLK + G  
Sbjct: 389 EDIAAIFNKADKGNTGTLKKKDFNSVVKDICQRYPQVELYLKKNKLKNIANLLKSANG-- 448

Query: 424 AKESIELNIEEFKLALSQVDSQMKNLPATAQVAAQQGAYLAKCFNRMEECEQNPEGPLRF 483
             E  ++NIE+FK ALS+VDSQMKNLPATAQVA+QQG YLAKCFN+ME+CE+ PEGPLRF
Sbjct: 449 --EDTQVNIEKFKQALSEVDSQMKNLPATAQVASQQGKYLAKCFNKMEKCEKKPEGPLRF 508

Query: 484 RGTGRHRFRPFRYKHLGQFAPLGGEQTAAQLPGDWVSIGHSSQWLWYSVYASKQVSWRTR 543
           RG GRHRF+PFRY+H G FAPLGGEQTAA+LPGDWVSIGHSSQWLWYSVYASK VSWRTR
Sbjct: 509 RGEGRHRFQPFRYRHFGSFAPLGGEQTAAELPGDWVSIGHSSQWLWYSVYASKLVSWRTR 568

Query: 544 ALVVTDWTRRFVFGRDSSRI 564
            LV++DWTRRFVFGRDSS I
Sbjct: 569 MLVISDWTRRFVFGRDSSSI 582

BLAST of MC03g0833 vs. TAIR 10
Match: AT4G28220.1 (NAD(P)H dehydrogenase B1 )

HSP 1 Score: 775.8 bits (2002), Expect = 2.4e-224
Identity = 373/558 (66.85%), Postives = 456/558 (81.72%), Query Frame = 0

Query: 6   SGGSLVAYADASPINVAPSTSIASFKADVDEKKKKVVVLGTGWAGTSFLKNIKDASYEIQ 65
           SGGS+VAYADA+                 + KKKKVVVLGTGWAG SFLK++   SY++Q
Sbjct: 27  SGGSIVAYADAN----------EEANKKEEHKKKKVVVLGTGWAGISFLKDLDITSYDVQ 86

Query: 66  VISPRNYFAFTPLLPSVTCGTVEARSIVEPIRNIVRKKGVDIIFNEAECYKIDAENRKLY 125
           V+SP+NYFAFTPLLPSVTCGTVEARSIVE +RNI +KK  +I   EA+C+KID  N+K++
Sbjct: 87  VVSPQNYFAFTPLLPSVTCGTVEARSIVESVRNITKKKNGEIELWEADCFKIDHVNQKVH 146

Query: 126 CRSNANNNLNGKKEFVVDYDYLVIAVGAQVNTFNTPGVVENCHFLKEVEDAQRIRRTVID 185
           CR    ++    +EF + YDYL++AVGAQVNTF TPGV+ENCHFLKEVEDAQRIRR VID
Sbjct: 147 CRPVFKDDPEASQEFSLGYDYLIVAVGAQVNTFGTPGVLENCHFLKEVEDAQRIRRGVID 206

Query: 186 CFERASLPTLNEEDRKRILHFAVVGGGPTGVEFAAELHDFVNEDLVKLYPGLKDFVKITL 245
           CFE+A LP L EE R+R LHF +VGGGPTGVEFAAELHDF+ ED+ K+YP +K+ VKITL
Sbjct: 207 CFEKAILPGLTEEQRRRKLHFVIVGGGPTGVEFAAELHDFIIEDITKIYPSVKELVKITL 266

Query: 246 LEAGDHILNMFDKRITTFAEEKFRRDGIDVKTGSIVIKVTDKEISTKEMKSGEVSSMPYG 305
           +++GDHILN FD+RI++FAE+KF RDGIDV+TG  V+ VTDK+I+ K   SGE+ S+P+G
Sbjct: 267 IQSGDHILNTFDERISSFAEQKFTRDGIDVQTGMRVMSVTDKDITVKVKSSGELVSIPHG 326

Query: 306 MTVWSTGIGTRPIIKDFMTQIGQVNRRALATDEWLRVEGCDNVYAIGDSATINQRRVMED 365
           + +WSTG+GTRP+I DFM Q+GQ  RRA+AT+EWL+V GC+NVYA+GD A+I QR+++ D
Sbjct: 327 LILWSTGVGTRPVISDFMEQVGQGGRRAVATNEWLQVTGCENVYAVGDCASIAQRKILGD 386

Query: 366 IAAIFSKADKDNSGTLTVKEFQEVINDICERYPQVELYLKNKQMHNIVDLLKGSKGDVAK 425
           IA IF  AD DNSGTLT++E + V++DI  RYPQVELYLK+K M +I DLL  S+G+  K
Sbjct: 387 IANIFKAADADNSGTLTMEELEGVVDDIIVRYPQVELYLKSKHMRHINDLLADSEGNARK 446

Query: 426 ESIELNIEEFKLALSQVDSQMKNLPATAQVAAQQGAYLAKCFNRMEECEQNPEGPLRFRG 485
              E++IE FKLALS+ DSQMK LPATAQVAAQQGAYLAKCFNRME+C++ PEGP RFR 
Sbjct: 447 ---EVDIEAFKLALSEADSQMKTLPATAQVAAQQGAYLAKCFNRMEQCKELPEGPKRFRT 506

Query: 486 TGRHRFRPFRYKHLGQFAPLGGEQTAAQLPGDWVSIGHSSQWLWYSVYASKQVSWRTRAL 545
            G H+FRPF+YKH GQFAPLGG+Q AA+LPGDWVS G S+QWLWYSVYASKQVSWRTRAL
Sbjct: 507 GGHHQFRPFQYKHFGQFAPLGGDQAAAELPGDWVSAGKSAQWLWYSVYASKQVSWRTRAL 566

Query: 546 VVTDWTRRFVFGRDSSRI 564
           VV+DWTRR++FGRDSSRI
Sbjct: 567 VVSDWTRRYIFGRDSSRI 571

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F4JJJ35.0e-25175.85External alternative NAD(P)H-ubiquinone oxidoreductase B3, mitochondrial OS=Arab... [more]
Q94BV78.0e-24976.39External alternative NAD(P)H-ubiquinone oxidoreductase B2, mitochondrial OS=Arab... [more]
Q9SKT71.0e-23269.11External alternative NAD(P)H-ubiquinone oxidoreductase B4, mitochondrial OS=Arab... [more]
M1BYJ76.1e-22566.25External alternative NAD(P)H-ubiquinone oxidoreductase B1, mitochondrial OS=Sola... [more]
Q9ST628.9e-22466.07External alternative NAD(P)H-ubiquinone oxidoreductase B1, mitochondrial OS=Sola... [more]
Match NameE-valueIdentityDescription
XP_022140190.10.099.47external alternative NAD(P)H-ubiquinone oxidoreductase B3, mitochondrial [Momord... [more]
XP_004146581.10.092.90external alternative NAD(P)H-ubiquinone oxidoreductase B2, mitochondrial [Cucumi... [more]
KAG7019646.10.092.90External alternative NAD(P)H-ubiquinone oxidoreductase B3, mitochondrial [Cucurb... [more]
XP_022927618.10.092.72external alternative NAD(P)H-ubiquinone oxidoreductase B2, mitochondrial [Cucurb... [more]
XP_008441960.10.092.72PREDICTED: external alternative NAD(P)H-ubiquinone oxidoreductase B2, mitochondr... [more]
Match NameE-valueIdentityDescription
A0A6J1CEF10.099.47NADH:ubiquinone reductase (non-electrogenic) OS=Momordica charantia OX=3673 GN=L... [more]
A0A0A0LV070.092.90NADH:ubiquinone reductase (non-electrogenic) OS=Cucumis sativus OX=3659 GN=Csa_1... [more]
A0A6J1ELH70.092.72NADH:ubiquinone reductase (non-electrogenic) OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1KKX90.092.72NADH:ubiquinone reductase (non-electrogenic) OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A1S3B4360.092.72NADH:ubiquinone reductase (non-electrogenic) OS=Cucumis melo OX=3656 GN=LOC10348... [more]
Match NameE-valueIdentityDescription
AT4G21490.13.5e-25275.85NAD(P)H dehydrogenase B3 [more]
AT4G05020.15.7e-25076.39NAD(P)H dehydrogenase B2 [more]
AT4G05020.22.1e-24471.64NAD(P)H dehydrogenase B2 [more]
AT2G20800.17.4e-23469.11NAD(P)H dehydrogenase B4 [more]
AT4G28220.12.4e-22466.85NAD(P)H dehydrogenase B1 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00368FADPNRcoord: 144..162
score: 24.89
coord: 41..60
score: 29.1
coord: 205..223
score: 41.96
NoneNo IPR availableGENE3D3.50.50.100coord: 30..375
e-value: 9.9E-135
score: 451.8
coord: 407..562
e-value: 4.1E-29
score: 103.5
NoneNo IPR availablePANTHERPTHR43706:SF22NAD(P)H DEHYDROGENASE B2coord: 6..563
IPR002048EF-hand domainSMARTSM00054efh_1coord: 365..393
e-value: 0.001
score: 28.3
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 361..396
score: 12.923368
IPR023753FAD/NAD(P)-binding domainPFAMPF07992Pyr_redox_2coord: 39..364
e-value: 1.0E-56
score: 192.4
IPR045024Alternative NADH dehydrogenasePANTHERPTHR43706NADH DEHYDROGENASEcoord: 6..563
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 374..386
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 357..449
IPR036188FAD/NAD(P)-binding domain superfamilySUPERFAMILY51905FAD/NAD(P)-binding domaincoord: 197..358
IPR036188FAD/NAD(P)-binding domain superfamilySUPERFAMILY51905FAD/NAD(P)-binding domaincoord: 38..257

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC03g0833.1MC03g0833.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006116 NADH oxidation
cellular_component GO:0005743 mitochondrial inner membrane
cellular_component GO:0005777 peroxisome
molecular_function GO:0005509 calcium ion binding
molecular_function GO:0050136 NADH dehydrogenase (quinone) activity
molecular_function GO:0003954 NADH dehydrogenase activity
molecular_function GO:0016491 oxidoreductase activity