MC03g0654 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC03g0654
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptiontranscription termination factor MTEF1, chloroplastic
LocationMC03: 13514394 .. 13515314 (-)
RNA-Seq ExpressionMC03g0654
SyntenyMC03g0654
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCTGCTGCAACTGCAACTGCTTTTCATTCTTCTTTCTGCTTCTCTTCTCACAAACTTTCTTCCTCTCCCTCAAATTCCCATTCCAACTCCCACCAACCAAACACTTTATTAGCAGCCAAACCCAAAACCCTCTTCCATAAGCATCCCCTCTACACCCCTCTTCACTCCACCCTCTCTTCCCAAGTCAAAGAGAAAATCCTGTGCCTTGAAATCATGGGTGTCGATGCTGGCAAGGCCCTCTCTCTCAATCCCTCTCTCCACTCTGTAACTCTTGAATCCATCCACTCTGTCATCTCCTTTCTCCAATCTAAAGGCATTCACCAGAAGGACTTCGCCAAAATCTTTGGAATGTGTCCCAAAATCCTCACCTCAGATGTCAAAACTGACCTCATCCCAGTTTTCAACTTCCTCTCTGAAGACCTTAAAATCCCAGATCAGAGCTTCAGAAGGACCATCAACAAGTGCCCAAGACTGCTCGCTTCAAGTGCCAAAGACCAGCTGAAGCCAGCTCTGTTTTACCTTCAGAGACTTGGGTTGAGGGATTTGGAGGCCTTGGCTTACCATGACTCTGTTCTGCTGGTTTCTAGTGTGGAGAGGACCTTGATTCCCAAACTCAAGTATTTGGAGAGTTTGGGATTCTCGAGGAGTGAAACTGTGGGGATGGTCTTGAGGTGTCCAGCACTGCTCACTTTCAGCATTGAAAATAACTTCAAGCCAAAGTTTGAGTACTTTTCTGGGGAGATGCATGGAAAATTGGAGGAGCTCAAGGACTTCCCTCAGTATTTTGCCTTCAGCTTGGAAAAGAGGATAAAGCCAAGGTATTTGGAGACTGTGCAGAGTGGAAAGAAAGTGCCTTTGTCACTTATGCTCAAGACCACAGATGATGAGTTTAGAGAATTGTTAAGAGAAGGCCAAGGG

mRNA sequence

ATGCCTGCTGCAACTGCAACTGCTTTTCATTCTTCTTTCTGCTTCTCTTCTCACAAACTTTCTTCCTCTCCCTCAAATTCCCATTCCAACTCCCACCAACCAAACACTTTATTAGCAGCCAAACCCAAAACCCTCTTCCATAAGCATCCCCTCTACACCCCTCTTCACTCCACCCTCTCTTCCCAAGTCAAAGAGAAAATCCTGTGCCTTGAAATCATGGGTGTCGATGCTGGCAAGGCCCTCTCTCTCAATCCCTCTCTCCACTCTGTAACTCTTGAATCCATCCACTCTGTCATCTCCTTTCTCCAATCTAAAGGCATTCACCAGAAGGACTTCGCCAAAATCTTTGGAATGTGTCCCAAAATCCTCACCTCAGATGTCAAAACTGACCTCATCCCAGTTTTCAACTTCCTCTCTGAAGACCTTAAAATCCCAGATCAGAGCTTCAGAAGGACCATCAACAAGTGCCCAAGACTGCTCGCTTCAAGTGCCAAAGACCAGCTGAAGCCAGCTCTGTTTTACCTTCAGAGACTTGGGTTGAGGGATTTGGAGGCCTTGGCTTACCATGACTCTGTTCTGCTGGTTTCTAGTGTGGAGAGGACCTTGATTCCCAAACTCAAGTATTTGGAGAGTTTGGGATTCTCGAGGAGTGAAACTGTGGGGATGGTCTTGAGGTGTCCAGCACTGCTCACTTTCAGCATTGAAAATAACTTCAAGCCAAAGTTTGAGTACTTTTCTGGGGAGATGCATGGAAAATTGGAGGAGCTCAAGGACTTCCCTCAGTATTTTGCCTTCAGCTTGGAAAAGAGGATAAAGCCAAGGTATTTGGAGACTGTGCAGAGTGGAAAGAAAGTGCCTTTGTCACTTATGCTCAAGACCACAGATGATGAGTTTAGAGAATTGTTAAGAGAAGGCCAAGGG

Coding sequence (CDS)

ATGCCTGCTGCAACTGCAACTGCTTTTCATTCTTCTTTCTGCTTCTCTTCTCACAAACTTTCTTCCTCTCCCTCAAATTCCCATTCCAACTCCCACCAACCAAACACTTTATTAGCAGCCAAACCCAAAACCCTCTTCCATAAGCATCCCCTCTACACCCCTCTTCACTCCACCCTCTCTTCCCAAGTCAAAGAGAAAATCCTGTGCCTTGAAATCATGGGTGTCGATGCTGGCAAGGCCCTCTCTCTCAATCCCTCTCTCCACTCTGTAACTCTTGAATCCATCCACTCTGTCATCTCCTTTCTCCAATCTAAAGGCATTCACCAGAAGGACTTCGCCAAAATCTTTGGAATGTGTCCCAAAATCCTCACCTCAGATGTCAAAACTGACCTCATCCCAGTTTTCAACTTCCTCTCTGAAGACCTTAAAATCCCAGATCAGAGCTTCAGAAGGACCATCAACAAGTGCCCAAGACTGCTCGCTTCAAGTGCCAAAGACCAGCTGAAGCCAGCTCTGTTTTACCTTCAGAGACTTGGGTTGAGGGATTTGGAGGCCTTGGCTTACCATGACTCTGTTCTGCTGGTTTCTAGTGTGGAGAGGACCTTGATTCCCAAACTCAAGTATTTGGAGAGTTTGGGATTCTCGAGGAGTGAAACTGTGGGGATGGTCTTGAGGTGTCCAGCACTGCTCACTTTCAGCATTGAAAATAACTTCAAGCCAAAGTTTGAGTACTTTTCTGGGGAGATGCATGGAAAATTGGAGGAGCTCAAGGACTTCCCTCAGTATTTTGCCTTCAGCTTGGAAAAGAGGATAAAGCCAAGGTATTTGGAGACTGTGCAGAGTGGAAAGAAAGTGCCTTTGTCACTTATGCTCAAGACCACAGATGATGAGTTTAGAGAATTGTTAAGAGAAGGCCAAGGG

Protein sequence

MPAATATAFHSSFCFSSHKLSSSPSNSHSNSHQPNTLLAAKPKTLFHKHPLYTPLHSTLSSQVKEKILCLEIMGVDAGKALSLNPSLHSVTLESIHSVISFLQSKGIHQKDFAKIFGMCPKILTSDVKTDLIPVFNFLSEDLKIPDQSFRRTINKCPRLLASSAKDQLKPALFYLQRLGLRDLEALAYHDSVLLVSSVERTLIPKLKYLESLGFSRSETVGMVLRCPALLTFSIENNFKPKFEYFSGEMHGKLEELKDFPQYFAFSLEKRIKPRYLETVQSGKKVPLSLMLKTTDDEFRELLREGQG
Homology
[Close]
BLAST of MC03g0654 vs. ExPASy Swiss-Prot
Match: Q84X53 (Transcription termination factor MTEF1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MTERF1 PE=2 SV=2)

HSP 1 Score: 221.9 bits (564), Expect = 1.0e-56
Identity = 115/242 (47.52%), Postives = 166/242 (68.60%), Query Frame = 0 

Query: 64  KEKILCLEIMGVDAGKALSLNPSLHSVTLESIHSVISFLQSKGIHQKDFAKIFGMCPKIL 123
           +EK++ L+ + VD  KAL +NPSL S  + S+ SV + L S G+ +    +I  M P +L
Sbjct: 34  REKLIYLQDLNVDPHKALRVNPSLRSAPISSVVSVETLLSSTGLSRPAVGRILDMFPDLL 93

Query: 124 TSDVKTDLIPVFNFLSEDLKIPDQSFRRTINKCPRLLASSAKDQLKPALFYLQRLGLRDL 183
           TSD +++++PV  FLS ++ I +Q   ++I++CPRLL SS   QL+PAL +L+ LG    
Sbjct: 94  TSDPESEILPVLRFLSNEISISEQDIPKSISRCPRLLISSVDYQLRPALTFLKTLGFVGR 153

Query: 184 EALAYHDSVLLVSSVERTLIPKLKYL-ESLGFSRSETVGMVLRCPALLTFSIENNFKPKF 243
           + +   ++VLLVS+VERTLIPK++YL E LGF+R E   MV+R PALLT+S++NN  PK 
Sbjct: 154 DTITSRNTVLLVSNVERTLIPKIEYLEEGLGFTREEVAKMVVRSPALLTYSVDNNLVPKV 213

Query: 244 EYFSGEMHGKLEELKDFPQYFAFSLEKRIKPRYLETVQSGKKVPLSLMLKTTDDEFRELL 303
           E+F  EM G ++ELK FPQYF+FSLE++IKPR+    + G  +PLS MLK +D +F   L
Sbjct: 214 EFFIEEMRGDVKELKRFPQYFSFSLERKIKPRHRLLKEHGILMPLSEMLKVSDGQFNHWL 273

Query: 304 RE 305
            E
Sbjct: 274 LE 275

[Close]
BLAST of MC03g0654 vs. ExPASy Swiss-Prot
Match: F4JVI3 (Transcription termination factor MTERF5, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MTERF5 PE=1 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 8.7e-24
Identity = 75/243 (30.86%), Postives = 130/243 (53.50%), Query Frame = 0 

Query: 63  VKEKILCLEIMGVDAGKALSLNPSLHSVTL-ESIHSVISFLQSKGIHQKDFAKIFGMCPK 122
           V E +L L I   D    L   P +  ++L +++   ++FL++ GI +  +AKI    P 
Sbjct: 235 VVEFLLDLGIPKSDIPTILCKRPQICGISLTDNLKPTMAFLETLGIDKNQWAKIISRFPA 294

Query: 123 ILTSDVKTDLIPVFNFLSEDLKIPDQSFRRTINKCPRLLASSAKDQLKPALFYLQRLGLR 182
           ILT   +  L     FLS+   + ++   R + +CP +++ S +D+L+P + Y + L + 
Sbjct: 295 ILTYS-RQKLTSTVEFLSQ-TGLTEEQIGRILTRCPNIMSYSVEDKLRPTMEYFRSLNV- 354

Query: 183 DLEALAYHDSVLLVSSVERTLIPKLKYLESLGFSRSETVGMVLRCPALLTFSIENNFKPK 242
           D+  L +        S+E  L P  ++    GF   E   M+ R  AL TFS++ N  PK
Sbjct: 355 DVAVLLHRCPQTFGLSIESNLKPVTEFFLEKGFGLDEIGIMISRYGALYTFSLKENVMPK 414

Query: 243 FEYFSGEMHGKLEELKDFPQYFAFSLEKRIKPRYLETVQSGKKVPLSLMLKTTDDEFREL 302
           ++YF    + K  EL  FPQ+F +SL++RIKPRY    +SG ++ L+ +L  +  EF ++
Sbjct: 415 WDYFQTMDYPK-SELVKFPQFFGYSLQERIKPRYELVQRSGVRLLLNQVLSLSGIEFEKV 473

Query: 303 LRE 305
           +++
Sbjct: 475 VKK 473

[Close]
BLAST of MC03g0654 vs. ExPASy Swiss-Prot
Match: F4IHL3 (Transcription termination factor MTERF2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MTERF2 PE=3 SV=1)

HSP 1 Score: 103.6 bits (257), Expect = 4.1e-21
Identity = 76/261 (29.12%), Postives = 134/261 (51.34%), Query Frame = 0 

Query: 52  YTPLHSTLSSQVKE-KILCLEIMGV---DAGKALSLNPSLHSVTLES-IHSVISFLQSKG 111
           Y  +    S QV E KI  L+  G+   + G+ L+  P L   ++E     ++ +    G
Sbjct: 340 YPKIIGFFSFQVMEKKINYLKEFGLSTEEVGRLLAYKPHLMGCSIEERWKPLVKYFYYLG 399

Query: 112 IHQKDFAKIFGMCPKILTSDVKTDLIPVFNFLSEDLKIPDQSFRRTINKCPRLLASSAKD 171
           I ++   +I  + P +   D++  + P   FL E + IP+++    + K P LL +S   
Sbjct: 400 IPKEGMKRILVVKPILYCIDLEKTIAPKVRFLQE-MGIPNEAIGNMLVKFPSLLTNSLYK 459

Query: 172 QLKP-ALFYLQRLGL--RDLEALAYHDSVLLVSSVERTLIPKLKYLESLGFSRSETVGMV 231
           +++P  +F L R G+  +D+  +   D  LL  S+   L P ++Y  SLG    +   M+
Sbjct: 460 KIRPVVIFLLTRAGVTQKDIGKVIAMDPALLGCSIGTKLEPNMRYYISLGIRFYQLGEMI 519

Query: 232 LRCPALLTFSIENNFKPKFEYFSGEMHGKLEELKDFPQYFAFSLEKRIKPRYLETVQSGK 291
              P LL ++++ N +PK+ Y    M   L++L +FP++F++SLE+RI PR+   V++  
Sbjct: 520 ADFPMLLRYNVD-NLRPKYRYLRRTMIRPLQDLIEFPRFFSYSLERRIIPRHTIMVENRV 579

Query: 292 KVPLSLMLKTTDDEFRELLRE 305
              L  ML  TD+EF   +R+
Sbjct: 580 NFKLRYMLACTDEEFERRVRD 598

[Close]
BLAST of MC03g0654 vs. ExPASy Swiss-Prot
Match: Q9ZT96 (Transcription termination factor MTERF4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MTERF4 PE=1 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 2.1e-17
Identity = 71/270 (26.30%), Postives = 118/270 (43.70%), Query Frame = 0 

Query: 70  LEIMGVDAGKALSLNPSLHSVTLESIHSV-ISFLQSKGIHQKDFAKIFGMCPKILTSDVK 129
           L+I   D  + L   P +    LE   S  +++L   G+ +++   I    P+IL   V 
Sbjct: 225 LDIKPSDVPRVLERYPEVLGFKLEGTMSTSVAYLVGIGVARREIGGILTRYPEILGMRVA 284

Query: 130 TDLIPVFNFLSEDLKIPDQSFRRTINKCPRLLASSAKDQLKPALFYLQRLGLRDL---EA 189
             + P+  +L E L IP  +  R I K P +L     D +KP +  LQ   +R+      
Sbjct: 285 RIIKPLVEYL-EVLGIPRLAAARLIEKRPHILGFELDDTVKPNVQILQDFNVRETSLPSI 344

Query: 190 LAYHDSVL-----------------------------------LVSSVERTLIPKLKYLE 249
           +A +  ++                                    VS  E  ++  + +L 
Sbjct: 345 IAQYPEIIGIDLKPKLDTQRKLLCSAIHLNPEDLGSLIERMPQFVSLSESPMLKHIDFLT 404

Query: 250 SLGFSRSETVGMVLRCPALLTFSIENNFKPKFEYFSGEMHGKLEELKDFPQYFAFSLEKR 301
             GFS  +T  MV+ CP +L  ++    K  FEYF  EM   L++L DFP +F + LE  
Sbjct: 405 KCGFSIDQTREMVIGCPQVLALNL-GIMKLSFEYFQKEMKRPLQDLVDFPAFFTYGLEST 464

[Close]
BLAST of MC03g0654 vs. ExPASy Swiss-Prot
Match: Q9FM80 (Transcription termination factor MTERF9, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MTERF9 PE=2 SV=1)

HSP 1 Score: 87.4 bits (215), Expect = 3.0e-16
Identity = 61/265 (23.02%), Postives = 122/265 (46.04%), Query Frame = 0 

Query: 45  LFHKHPLYTPLHSTLSSQVKEKILCLEIMGVDAGKALSLNPSLHSVTLE-SIHSVISFLQ 104
           ++ +H     ++   + +  + +L + +   D  + L   P +   T+E ++ + ISFL 
Sbjct: 204 MYERHMPSLQINVFSAQERLDYLLSVGVKHRDIKRMLLRQPQILQYTVENNLKAHISFLM 263

Query: 105 SKGIHQKDFAKIFGMCPKILTSDVKTDLIPVFNFLSEDLKIPDQSFRRTINKCPRLLASS 164
             GI      +I    P + +  V+  L P   +L E++ I +    + +   P++L   
Sbjct: 264 GLGIPNSKIGQIVAATPSLFSYSVENSLRPTIRYLIEEVGIKETDVGKVVQLSPQILVQR 323

Query: 165 AKDQLKPALFYLQRLGLRDLEALAYHDSV---------LLVSSVERTLIPKLKYLESLGF 224
                     +L +      E  A  DSV         LL  S++   +P++ +L S+G 
Sbjct: 324 LDITWNTRYMFLSK------ELGAPRDSVVKMVKKHPQLLHYSIDDGFLPRINFLRSIGM 383

Query: 225 SRSETVGMVLRCPALLTFSIENNFKPKFEYFSGEMHGKLEELKDFPQYFAFSLEKRIKP- 284
             S+ + ++     +L+ S+E+N KPK+ Y   E++ ++  L  +P Y + SL++RI+P 
Sbjct: 384 CNSDILKVLTSLTQVLSLSLEDNLKPKYMYLVNELNNEVHILTKYPMYLSLSLDQRIRPR 443

Query: 285 -RYLETVQSGKKVPLSLMLKTTDDE 298
            R+L  ++  +K P  L     +DE
Sbjct: 444 HRFLVELKKVRKGPFPLSSLVPNDE 462

[Close]
BLAST of MC03g0654 vs. NCBI nr
Match: XP_022139889.1 (transcription termination factor MTEF1, chloroplastic [Momordica charantia])

HSP 1 Score: 598 bits (1541), Expect = 3.30e-215
Identity = 307/307 (100.00%), Postives = 307/307 (100.00%), Query Frame = 0 

Query: 1   MPAATATAFHSSFCFSSHKLSSSPSNSHSNSHQPNTLLAAKPKTLFHKHPLYTPLHSTLS 60
           MPAATATAFHSSFCFSSHKLSSSPSNSHSNSHQPNTLLAAKPKTLFHKHPLYTPLHSTLS
Sbjct: 1   MPAATATAFHSSFCFSSHKLSSSPSNSHSNSHQPNTLLAAKPKTLFHKHPLYTPLHSTLS 60

Query: 61  SQVKEKILCLEIMGVDAGKALSLNPSLHSVTLESIHSVISFLQSKGIHQKDFAKIFGMCP 120
           SQVKEKILCLEIMGVDAGKALSLNPSLHSVTLESIHSVISFLQSKGIHQKDFAKIFGMCP
Sbjct: 61  SQVKEKILCLEIMGVDAGKALSLNPSLHSVTLESIHSVISFLQSKGIHQKDFAKIFGMCP 120

Query: 121 KILTSDVKTDLIPVFNFLSEDLKIPDQSFRRTINKCPRLLASSAKDQLKPALFYLQRLGL 180
           KILTSDVKTDLIPVFNFLSEDLKIPDQSFRRTINKCPRLLASSAKDQLKPALFYLQRLGL
Sbjct: 121 KILTSDVKTDLIPVFNFLSEDLKIPDQSFRRTINKCPRLLASSAKDQLKPALFYLQRLGL 180

Query: 181 RDLEALAYHDSVLLVSSVERTLIPKLKYLESLGFSRSETVGMVLRCPALLTFSIENNFKP 240
           RDLEALAYHDSVLLVSSVERTLIPKLKYLESLGFSRSETVGMVLRCPALLTFSIENNFKP
Sbjct: 181 RDLEALAYHDSVLLVSSVERTLIPKLKYLESLGFSRSETVGMVLRCPALLTFSIENNFKP 240

Query: 241 KFEYFSGEMHGKLEELKDFPQYFAFSLEKRIKPRYLETVQSGKKVPLSLMLKTTDDEFRE 300
           KFEYFSGEMHGKLEELKDFPQYFAFSLEKRIKPRYLETVQSGKKVPLSLMLKTTDDEFRE
Sbjct: 241 KFEYFSGEMHGKLEELKDFPQYFAFSLEKRIKPRYLETVQSGKKVPLSLMLKTTDDEFRE 300

Query: 301 LLREGQG 307
           LLREGQG
Sbjct: 301 LLREGQG 307

[Close]
BLAST of MC03g0654 vs. NCBI nr
Match: XP_038895185.1 (transcription termination factor MTEF1, chloroplastic [Benincasa hispida])

HSP 1 Score: 542 bits (1396), Expect = 3.32e-193
Identity = 279/307 (90.88%), Postives = 291/307 (94.79%), Query Frame = 0 

Query: 1   MPAATATAFHSSFCFSSHKLSSSPSNSHSNSHQPNTLLAAKPKTLFHKHPLYTPLHSTLS 60
           MP++TATAFHSSFCFSSHKLSSS S+SH    QPNT L+AKPKTLFHKHPLYTPLHSTLS
Sbjct: 1   MPSSTATAFHSSFCFSSHKLSSSSSSSH----QPNTFLSAKPKTLFHKHPLYTPLHSTLS 60

Query: 61  SQVKEKILCLEIMGVDAGKALSLNPSLHSVTLESIHSVISFLQSKGIHQKDFAKIFGMCP 120
           SQ+KEKILCLEIMGVDAGKALS NPSLHSVTLESIHSVISFLQSKGIHQKDFAKIFGMCP
Sbjct: 61  SQIKEKILCLEIMGVDAGKALSQNPSLHSVTLESIHSVISFLQSKGIHQKDFAKIFGMCP 120

Query: 121 KILTSDVKTDLIPVFNFLSEDLKIPDQSFRRTINKCPRLLASSAKDQLKPALFYLQRLGL 180
           KILTSDVKTDLIPVFNFLSEDLKIPDQ+FRR INKCPRLLASS KDQLKPALFYLQRLGL
Sbjct: 121 KILTSDVKTDLIPVFNFLSEDLKIPDQNFRRAINKCPRLLASSPKDQLKPALFYLQRLGL 180

Query: 181 RDLEALAYHDSVLLVSSVERTLIPKLKYLESLGFSRSETVGMVLRCPALLTFSIENNFKP 240
           +DLEALAY DSVLLVSSVE+TLIPKLKYLESLG SRSETVGMVLRCPALLTFSIENNFKP
Sbjct: 181 KDLEALAYRDSVLLVSSVEKTLIPKLKYLESLGLSRSETVGMVLRCPALLTFSIENNFKP 240

Query: 241 KFEYFSGEMHGKLEELKDFPQYFAFSLEKRIKPRYLETVQSGKKVPLSLMLKTTDDEFRE 300
           KFEYFS EMH KLEELKDFPQYFAFSLEKRIKPRY+ETV+SGKKVPL LMLKTTDDEFR+
Sbjct: 241 KFEYFSVEMHRKLEELKDFPQYFAFSLEKRIKPRYVETVRSGKKVPLPLMLKTTDDEFRQ 300

Query: 301 LLREGQG 307
           LL +G G
Sbjct: 301 LLTQGDG 303

[Close]
BLAST of MC03g0654 vs. NCBI nr
Match: XP_008444692.1 (PREDICTED: transcription termination factor MTEF1, chloroplastic [Cucumis melo] >KAA0054024.1 transcription termination factor MTEF1 [Cucumis melo var. makuwa] >TYK20710.1 transcription termination factor MTEF1 [Cucumis melo var. makuwa])

HSP 1 Score: 530 bits (1366), Expect = 1.28e-188
Identity = 276/308 (89.61%), Postives = 290/308 (94.16%), Query Frame = 0 

Query: 1   MPAATATAFHSSFCFSSH-KLSSSPSNSHSNSHQPNTLLAAKPKTLFHKHPLYTPLHSTL 60
           M +++ATAFHSSFCFSSH KLSSSPSNSH    QPNT L+ KPKTL HKHPLYTPLHSTL
Sbjct: 1   MSSSSATAFHSSFCFSSHNKLSSSPSNSH----QPNTSLSPKPKTLLHKHPLYTPLHSTL 60

Query: 61  SSQVKEKILCLEIMGVDAGKALSLNPSLHSVTLESIHSVISFLQSKGIHQKDFAKIFGMC 120
           SSQ KEKILCLEIMGVD+GKALS NPSLH VTLESIHSVISFLQSKGIHQKDFAKIFGMC
Sbjct: 61  SSQTKEKILCLEIMGVDSGKALSQNPSLHCVTLESIHSVISFLQSKGIHQKDFAKIFGMC 120

Query: 121 PKILTSDVKTDLIPVFNFLSEDLKIPDQSFRRTINKCPRLLASSAKDQLKPALFYLQRLG 180
           PKILTSDVKTDLIPVFNFLSEDLKIPDQ+FR+ INKCPRLLASSA+DQLKPALFYLQRLG
Sbjct: 121 PKILTSDVKTDLIPVFNFLSEDLKIPDQNFRKAINKCPRLLASSAEDQLKPALFYLQRLG 180

Query: 181 LRDLEALAYHDSVLLVSSVERTLIPKLKYLESLGFSRSETVGMVLRCPALLTFSIENNFK 240
           L+DLEALAYHDSVLLVSSVE+TLIPKLKYLESLGF+RSETVGMVLRCPALLTFSIENNFK
Sbjct: 181 LKDLEALAYHDSVLLVSSVEKTLIPKLKYLESLGFTRSETVGMVLRCPALLTFSIENNFK 240

Query: 241 PKFEYFSGEMHGKLEELKDFPQYFAFSLEKRIKPRYLETVQSGKKVPLSLMLKTTDDEFR 300
           PKFEYFS EMH KLEELKDFPQYFAFSLEKRIKPR++ETV+SGKKVPLSLMLKTTD EFR
Sbjct: 241 PKFEYFSVEMHRKLEELKDFPQYFAFSLEKRIKPRHVETVESGKKVPLSLMLKTTDVEFR 300

Query: 301 ELLREGQG 307
           ELL EG G
Sbjct: 301 ELLAEGGG 304

[Close]
BLAST of MC03g0654 vs. NCBI nr
Match: XP_004154376.1 (transcription termination factor MTEF1, chloroplastic [Cucumis sativus] >KGN65875.1 hypothetical protein Csa_023269 [Cucumis sativus])

HSP 1 Score: 530 bits (1366), Expect = 1.28e-188
Identity = 275/308 (89.29%), Postives = 290/308 (94.16%), Query Frame = 0 

Query: 1   MPAATATAFHSSFCFSSH-KLSSSPSNSHSNSHQPNTLLAAKPKTLFHKHPLYTPLHSTL 60
           M +++ATAFHSSFCFSSH KLSSSPSNSH    QPNT L+ KPKTL HKHPLYTPLHST+
Sbjct: 1   MSSSSATAFHSSFCFSSHNKLSSSPSNSH----QPNTSLSPKPKTLLHKHPLYTPLHSTV 60

Query: 61  SSQVKEKILCLEIMGVDAGKALSLNPSLHSVTLESIHSVISFLQSKGIHQKDFAKIFGMC 120
           SSQ KEKILCLEIMGVD+GKALS NPSLHSVTLESIHSVISFLQSKGIHQKDFAKIFGMC
Sbjct: 61  SSQTKEKILCLEIMGVDSGKALSQNPSLHSVTLESIHSVISFLQSKGIHQKDFAKIFGMC 120

Query: 121 PKILTSDVKTDLIPVFNFLSEDLKIPDQSFRRTINKCPRLLASSAKDQLKPALFYLQRLG 180
           PKILTSDVKTDL+PVFNFLSEDLKIPDQ+FR+ INKCPRLLASSA+DQLKPALFYLQRLG
Sbjct: 121 PKILTSDVKTDLVPVFNFLSEDLKIPDQNFRKAINKCPRLLASSAEDQLKPALFYLQRLG 180

Query: 181 LRDLEALAYHDSVLLVSSVERTLIPKLKYLESLGFSRSETVGMVLRCPALLTFSIENNFK 240
           L+DLEALAYHDSVLLVSSVE+TLIPKLKYLESLGF+RSE VGMVLRCPALLTFSIENNFK
Sbjct: 181 LKDLEALAYHDSVLLVSSVEKTLIPKLKYLESLGFTRSEIVGMVLRCPALLTFSIENNFK 240

Query: 241 PKFEYFSGEMHGKLEELKDFPQYFAFSLEKRIKPRYLETVQSGKKVPLSLMLKTTDDEFR 300
           PKFEYFS EMH KLEELKDFPQYFAFSLEKRIKPRY+ETV+SGKKVPLSLMLKTTD EFR
Sbjct: 241 PKFEYFSVEMHKKLEELKDFPQYFAFSLEKRIKPRYVETVESGKKVPLSLMLKTTDVEFR 300

Query: 301 ELLREGQG 307
           ELL EG G
Sbjct: 301 ELLAEGGG 304

[Close]
BLAST of MC03g0654 vs. NCBI nr
Match: KAG6583877.1 (Transcription termination factor MTEF1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 515 bits (1327), Expect = 9.69e-183
Identity = 269/307 (87.62%), Postives = 281/307 (91.53%), Query Frame = 0 

Query: 1   MPAATATAFHSSFCFSSHKLSSSPSNSHSNSHQPNTLLAAKPKTLFHKHPLYTPLHSTLS 60
           MPAA   AF SSFCFSSH+LS+SPSNS     QPNT LAAKPK+LFHKHPLYTPLHSTLS
Sbjct: 1   MPAA---AFPSSFCFSSHELSASPSNSQ----QPNTFLAAKPKSLFHKHPLYTPLHSTLS 60

Query: 61  SQVKEKILCLEIMGVDAGKALSLNPSLHSVTLESIHSVISFLQSKGIHQKDFAKIFGMCP 120
           SQ+KEKILCLEIMGVDAGKALS NPSLHSVTLESIHSVISFLQSKGIHQKDFAKIFGMCP
Sbjct: 61  SQIKEKILCLEIMGVDAGKALSQNPSLHSVTLESIHSVISFLQSKGIHQKDFAKIFGMCP 120

Query: 121 KILTSDVKTDLIPVFNFLSEDLKIPDQSFRRTINKCPRLLASSAKDQLKPALFYLQRLGL 180
           KILTSDVK DLIPVF+FLSEDLKIPDQ+FRR INKCPRLLASSAKDQLKPALFYLQRLG 
Sbjct: 121 KILTSDVKADLIPVFSFLSEDLKIPDQNFRRVINKCPRLLASSAKDQLKPALFYLQRLGF 180

Query: 181 RDLEALAYHDSVLLVSSVERTLIPKLKYLESLGFSRSETVGMVLRCPALLTFSIENNFKP 240
           +DLEALAY DS LLVSSVE+TLIPKLKYL+SLGFSRSETVGMVLRCPALLTFSIENN KP
Sbjct: 181 KDLEALAYQDSALLVSSVEKTLIPKLKYLQSLGFSRSETVGMVLRCPALLTFSIENNLKP 240

Query: 241 KFEYFSGEMHGKLEELKDFPQYFAFSLEKRIKPRYLETVQSGKKVPLSLMLKTTDDEFRE 300
           KFEYFSGEM  K+EELKDFPQYF FSLEKRIKPRY+ETVQSGK V L LMLK TDDEF+E
Sbjct: 241 KFEYFSGEMQRKVEELKDFPQYFGFSLEKRIKPRYVETVQSGKNVALPLMLKATDDEFKE 300

Query: 301 LLREGQG 307
           LLRE  G
Sbjct: 301 LLREESG 300

[Close]
BLAST of MC03g0654 vs. ExPASy TrEMBL
Match: A0A6J1CE81 (transcription termination factor MTEF1, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111010694 PE=3 SV=1)

HSP 1 Score: 598 bits (1541), Expect = 1.60e-215
Identity = 307/307 (100.00%), Postives = 307/307 (100.00%), Query Frame = 0 

Query: 1   MPAATATAFHSSFCFSSHKLSSSPSNSHSNSHQPNTLLAAKPKTLFHKHPLYTPLHSTLS 60
           MPAATATAFHSSFCFSSHKLSSSPSNSHSNSHQPNTLLAAKPKTLFHKHPLYTPLHSTLS
Sbjct: 1   MPAATATAFHSSFCFSSHKLSSSPSNSHSNSHQPNTLLAAKPKTLFHKHPLYTPLHSTLS 60

Query: 61  SQVKEKILCLEIMGVDAGKALSLNPSLHSVTLESIHSVISFLQSKGIHQKDFAKIFGMCP 120
           SQVKEKILCLEIMGVDAGKALSLNPSLHSVTLESIHSVISFLQSKGIHQKDFAKIFGMCP
Sbjct: 61  SQVKEKILCLEIMGVDAGKALSLNPSLHSVTLESIHSVISFLQSKGIHQKDFAKIFGMCP 120

Query: 121 KILTSDVKTDLIPVFNFLSEDLKIPDQSFRRTINKCPRLLASSAKDQLKPALFYLQRLGL 180
           KILTSDVKTDLIPVFNFLSEDLKIPDQSFRRTINKCPRLLASSAKDQLKPALFYLQRLGL
Sbjct: 121 KILTSDVKTDLIPVFNFLSEDLKIPDQSFRRTINKCPRLLASSAKDQLKPALFYLQRLGL 180

Query: 181 RDLEALAYHDSVLLVSSVERTLIPKLKYLESLGFSRSETVGMVLRCPALLTFSIENNFKP 240
           RDLEALAYHDSVLLVSSVERTLIPKLKYLESLGFSRSETVGMVLRCPALLTFSIENNFKP
Sbjct: 181 RDLEALAYHDSVLLVSSVERTLIPKLKYLESLGFSRSETVGMVLRCPALLTFSIENNFKP 240

Query: 241 KFEYFSGEMHGKLEELKDFPQYFAFSLEKRIKPRYLETVQSGKKVPLSLMLKTTDDEFRE 300
           KFEYFSGEMHGKLEELKDFPQYFAFSLEKRIKPRYLETVQSGKKVPLSLMLKTTDDEFRE
Sbjct: 241 KFEYFSGEMHGKLEELKDFPQYFAFSLEKRIKPRYLETVQSGKKVPLSLMLKTTDDEFRE 300

Query: 301 LLREGQG 307
           LLREGQG
Sbjct: 301 LLREGQG 307

[Close]
BLAST of MC03g0654 vs. ExPASy TrEMBL
Match: A0A5D3DB89 (Transcription termination factor MTEF1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold480G001070 PE=3 SV=1)

HSP 1 Score: 530 bits (1366), Expect = 6.21e-189
Identity = 276/308 (89.61%), Postives = 290/308 (94.16%), Query Frame = 0 

Query: 1   MPAATATAFHSSFCFSSH-KLSSSPSNSHSNSHQPNTLLAAKPKTLFHKHPLYTPLHSTL 60
           M +++ATAFHSSFCFSSH KLSSSPSNSH    QPNT L+ KPKTL HKHPLYTPLHSTL
Sbjct: 1   MSSSSATAFHSSFCFSSHNKLSSSPSNSH----QPNTSLSPKPKTLLHKHPLYTPLHSTL 60

Query: 61  SSQVKEKILCLEIMGVDAGKALSLNPSLHSVTLESIHSVISFLQSKGIHQKDFAKIFGMC 120
           SSQ KEKILCLEIMGVD+GKALS NPSLH VTLESIHSVISFLQSKGIHQKDFAKIFGMC
Sbjct: 61  SSQTKEKILCLEIMGVDSGKALSQNPSLHCVTLESIHSVISFLQSKGIHQKDFAKIFGMC 120

Query: 121 PKILTSDVKTDLIPVFNFLSEDLKIPDQSFRRTINKCPRLLASSAKDQLKPALFYLQRLG 180
           PKILTSDVKTDLIPVFNFLSEDLKIPDQ+FR+ INKCPRLLASSA+DQLKPALFYLQRLG
Sbjct: 121 PKILTSDVKTDLIPVFNFLSEDLKIPDQNFRKAINKCPRLLASSAEDQLKPALFYLQRLG 180

Query: 181 LRDLEALAYHDSVLLVSSVERTLIPKLKYLESLGFSRSETVGMVLRCPALLTFSIENNFK 240
           L+DLEALAYHDSVLLVSSVE+TLIPKLKYLESLGF+RSETVGMVLRCPALLTFSIENNFK
Sbjct: 181 LKDLEALAYHDSVLLVSSVEKTLIPKLKYLESLGFTRSETVGMVLRCPALLTFSIENNFK 240

Query: 241 PKFEYFSGEMHGKLEELKDFPQYFAFSLEKRIKPRYLETVQSGKKVPLSLMLKTTDDEFR 300
           PKFEYFS EMH KLEELKDFPQYFAFSLEKRIKPR++ETV+SGKKVPLSLMLKTTD EFR
Sbjct: 241 PKFEYFSVEMHRKLEELKDFPQYFAFSLEKRIKPRHVETVESGKKVPLSLMLKTTDVEFR 300

Query: 301 ELLREGQG 307
           ELL EG G
Sbjct: 301 ELLAEGGG 304

[Close]
BLAST of MC03g0654 vs. ExPASy TrEMBL
Match: A0A0A0LVD4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G535790 PE=3 SV=1)

HSP 1 Score: 530 bits (1366), Expect = 6.21e-189
Identity = 275/308 (89.29%), Postives = 290/308 (94.16%), Query Frame = 0 

Query: 1   MPAATATAFHSSFCFSSH-KLSSSPSNSHSNSHQPNTLLAAKPKTLFHKHPLYTPLHSTL 60
           M +++ATAFHSSFCFSSH KLSSSPSNSH    QPNT L+ KPKTL HKHPLYTPLHST+
Sbjct: 1   MSSSSATAFHSSFCFSSHNKLSSSPSNSH----QPNTSLSPKPKTLLHKHPLYTPLHSTV 60

Query: 61  SSQVKEKILCLEIMGVDAGKALSLNPSLHSVTLESIHSVISFLQSKGIHQKDFAKIFGMC 120
           SSQ KEKILCLEIMGVD+GKALS NPSLHSVTLESIHSVISFLQSKGIHQKDFAKIFGMC
Sbjct: 61  SSQTKEKILCLEIMGVDSGKALSQNPSLHSVTLESIHSVISFLQSKGIHQKDFAKIFGMC 120

Query: 121 PKILTSDVKTDLIPVFNFLSEDLKIPDQSFRRTINKCPRLLASSAKDQLKPALFYLQRLG 180
           PKILTSDVKTDL+PVFNFLSEDLKIPDQ+FR+ INKCPRLLASSA+DQLKPALFYLQRLG
Sbjct: 121 PKILTSDVKTDLVPVFNFLSEDLKIPDQNFRKAINKCPRLLASSAEDQLKPALFYLQRLG 180

Query: 181 LRDLEALAYHDSVLLVSSVERTLIPKLKYLESLGFSRSETVGMVLRCPALLTFSIENNFK 240
           L+DLEALAYHDSVLLVSSVE+TLIPKLKYLESLGF+RSE VGMVLRCPALLTFSIENNFK
Sbjct: 181 LKDLEALAYHDSVLLVSSVEKTLIPKLKYLESLGFTRSEIVGMVLRCPALLTFSIENNFK 240

Query: 241 PKFEYFSGEMHGKLEELKDFPQYFAFSLEKRIKPRYLETVQSGKKVPLSLMLKTTDDEFR 300
           PKFEYFS EMH KLEELKDFPQYFAFSLEKRIKPRY+ETV+SGKKVPLSLMLKTTD EFR
Sbjct: 241 PKFEYFSVEMHKKLEELKDFPQYFAFSLEKRIKPRYVETVESGKKVPLSLMLKTTDVEFR 300

Query: 301 ELLREGQG 307
           ELL EG G
Sbjct: 301 ELLAEGGG 304

[Close]
BLAST of MC03g0654 vs. ExPASy TrEMBL
Match: A0A1S3BBS5 (transcription termination factor MTEF1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103487950 PE=3 SV=1)

HSP 1 Score: 530 bits (1366), Expect = 6.21e-189
Identity = 276/308 (89.61%), Postives = 290/308 (94.16%), Query Frame = 0 

Query: 1   MPAATATAFHSSFCFSSH-KLSSSPSNSHSNSHQPNTLLAAKPKTLFHKHPLYTPLHSTL 60
           M +++ATAFHSSFCFSSH KLSSSPSNSH    QPNT L+ KPKTL HKHPLYTPLHSTL
Sbjct: 1   MSSSSATAFHSSFCFSSHNKLSSSPSNSH----QPNTSLSPKPKTLLHKHPLYTPLHSTL 60

Query: 61  SSQVKEKILCLEIMGVDAGKALSLNPSLHSVTLESIHSVISFLQSKGIHQKDFAKIFGMC 120
           SSQ KEKILCLEIMGVD+GKALS NPSLH VTLESIHSVISFLQSKGIHQKDFAKIFGMC
Sbjct: 61  SSQTKEKILCLEIMGVDSGKALSQNPSLHCVTLESIHSVISFLQSKGIHQKDFAKIFGMC 120

Query: 121 PKILTSDVKTDLIPVFNFLSEDLKIPDQSFRRTINKCPRLLASSAKDQLKPALFYLQRLG 180
           PKILTSDVKTDLIPVFNFLSEDLKIPDQ+FR+ INKCPRLLASSA+DQLKPALFYLQRLG
Sbjct: 121 PKILTSDVKTDLIPVFNFLSEDLKIPDQNFRKAINKCPRLLASSAEDQLKPALFYLQRLG 180

Query: 181 LRDLEALAYHDSVLLVSSVERTLIPKLKYLESLGFSRSETVGMVLRCPALLTFSIENNFK 240
           L+DLEALAYHDSVLLVSSVE+TLIPKLKYLESLGF+RSETVGMVLRCPALLTFSIENNFK
Sbjct: 181 LKDLEALAYHDSVLLVSSVEKTLIPKLKYLESLGFTRSETVGMVLRCPALLTFSIENNFK 240

Query: 241 PKFEYFSGEMHGKLEELKDFPQYFAFSLEKRIKPRYLETVQSGKKVPLSLMLKTTDDEFR 300
           PKFEYFS EMH KLEELKDFPQYFAFSLEKRIKPR++ETV+SGKKVPLSLMLKTTD EFR
Sbjct: 241 PKFEYFSVEMHRKLEELKDFPQYFAFSLEKRIKPRHVETVESGKKVPLSLMLKTTDVEFR 300

Query: 301 ELLREGQG 307
           ELL EG G
Sbjct: 301 ELLAEGGG 304

[Close]
BLAST of MC03g0654 vs. ExPASy TrEMBL
Match: A0A6J1EFX2 (transcription termination factor MTEF1, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111433963 PE=3 SV=1)

HSP 1 Score: 514 bits (1325), Expect = 9.46e-183
Identity = 269/307 (87.62%), Postives = 281/307 (91.53%), Query Frame = 0 

Query: 1   MPAATATAFHSSFCFSSHKLSSSPSNSHSNSHQPNTLLAAKPKTLFHKHPLYTPLHSTLS 60
           MPAA   AF SSFCFSSH+LS+SPSNS     QPNT LAAKPK+LFHKHPLYTPLHSTLS
Sbjct: 1   MPAA---AFPSSFCFSSHELSASPSNSQ----QPNTFLAAKPKSLFHKHPLYTPLHSTLS 60

Query: 61  SQVKEKILCLEIMGVDAGKALSLNPSLHSVTLESIHSVISFLQSKGIHQKDFAKIFGMCP 120
           SQ+KEKILCLEIMGVDAGKALS NPSLHSVTLESIHSVISFLQSKGIHQKDFAKIFGMCP
Sbjct: 61  SQIKEKILCLEIMGVDAGKALSQNPSLHSVTLESIHSVISFLQSKGIHQKDFAKIFGMCP 120

Query: 121 KILTSDVKTDLIPVFNFLSEDLKIPDQSFRRTINKCPRLLASSAKDQLKPALFYLQRLGL 180
           KILTSDVK DLIPVF+FLSEDLKIPDQ+FRR INKCPRLLASSAKDQLKPALFYLQRLG 
Sbjct: 121 KILTSDVKADLIPVFSFLSEDLKIPDQNFRRVINKCPRLLASSAKDQLKPALFYLQRLGF 180

Query: 181 RDLEALAYHDSVLLVSSVERTLIPKLKYLESLGFSRSETVGMVLRCPALLTFSIENNFKP 240
           +DLEALAY DS LLVSSVE+TLIPKLKYL+SLGFSRSETVGMVLRCPALLTFSIENN KP
Sbjct: 181 KDLEALAYQDSALLVSSVEKTLIPKLKYLQSLGFSRSETVGMVLRCPALLTFSIENNLKP 240

Query: 241 KFEYFSGEMHGKLEELKDFPQYFAFSLEKRIKPRYLETVQSGKKVPLSLMLKTTDDEFRE 300
           KFEYFSGEM  K+EELKDFPQYF FSLEKRIKPRY+ETVQSGK V L LMLK TDDEF+E
Sbjct: 241 KFEYFSGEMQRKVEELKDFPQYFGFSLEKRIKPRYVETVQSGKNVALPLMLKATDDEFKE 300

Query: 301 LLREGQG 307
           LLRE  G
Sbjct: 301 LLREEGG 300

[Close]
BLAST of MC03g0654 vs. TAIR 10
Match: AT2G34620.1 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 377.5 bits (968), Expect = 1.0e-104
Identity = 194/295 (65.76%), Postives = 232/295 (78.64%), Query Frame = 0 

Query: 11  SSFCFSSHKLSSSPSNSHSNSHQPNTLLAAKP---KTLFHKHPLYTPLHSTLSSQVKEKI 70
           SS  + S K  S P  S S      T L+ KP   KT  H HPL+T    T++ Q+KEKI
Sbjct: 6   SSSLYISPKKPSCPQQSQS------TFLSTKPTTIKTNLHSHPLFTVADQTVTLQMKEKI 65

Query: 71  LCLEIMGVDAGKALSLNPSLHSVTLESIHSVISFLQSKGIHQKDFAKIFGMCPKILTSDV 130
           LCLE+MG+D+GKALSLNP L S  L+SI SV+ FLQSKGI+  D  +I GMCPKILTSDV
Sbjct: 66  LCLELMGIDSGKALSLNPCLCSAPLDSIQSVLHFLQSKGIYPNDLPRILGMCPKILTSDV 125

Query: 131 KTDLIPVFNFLSEDLKIPDQSFRRTINKCPRLLASSAKDQLKPALFYLQRLGLRDLEALA 190
           +T+L PVF FLS DL +P+ +FRR I KCPRLL SS +DQLKPALFYLQRLGL+DLEALA
Sbjct: 126 RTELYPVFMFLSNDLHVPENAFRRVIKKCPRLLISSVEDQLKPALFYLQRLGLKDLEALA 185

Query: 191 YHDSVLLVSSVERTLIPKLKYLESLGFSRSETVGMVLRCPALLTFSIENNFKPKFEYFSG 250
           Y D +LLVSSVE TLIPKL++LES+GFSR E +GM+LRCPAL TFSIENNFKPK +YF  
Sbjct: 186 YQDPILLVSSVEHTLIPKLRFLESIGFSRPEAIGMILRCPALFTFSIENNFKPKLDYFMS 245

Query: 251 EMHGKLEELKDFPQYFAFSLEKRIKPRYLETVQSGKKVPLSLMLKTTDDEFRELL 303
           E+ GKLE LK+FPQYFAFSLEKRIKPR+LE+++ G ++PLSLMLK+TD+EF +LL
Sbjct: 246 EIKGKLENLKEFPQYFAFSLEKRIKPRHLESMERGLELPLSLMLKSTDEEFEQLL 294

[Close]
BLAST of MC03g0654 vs. TAIR 10
Match: AT2G03050.1 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 221.9 bits (564), Expect = 7.3e-58
Identity = 115/242 (47.52%), Postives = 166/242 (68.60%), Query Frame = 0 

Query: 64  KEKILCLEIMGVDAGKALSLNPSLHSVTLESIHSVISFLQSKGIHQKDFAKIFGMCPKIL 123
           +EK++ L+ + VD  KAL +NPSL S  + S+ SV + L S G+ +    +I  M P +L
Sbjct: 33  REKLIYLQDLNVDPHKALRVNPSLRSAPISSVVSVETLLSSTGLSRPAVGRILDMFPDLL 92

Query: 124 TSDVKTDLIPVFNFLSEDLKIPDQSFRRTINKCPRLLASSAKDQLKPALFYLQRLGLRDL 183
           TSD +++++PV  FLS ++ I +Q   ++I++CPRLL SS   QL+PAL +L+ LG    
Sbjct: 93  TSDPESEILPVLRFLSNEISISEQDIPKSISRCPRLLISSVDYQLRPALTFLKTLGFVGR 152

Query: 184 EALAYHDSVLLVSSVERTLIPKLKYL-ESLGFSRSETVGMVLRCPALLTFSIENNFKPKF 243
           + +   ++VLLVS+VERTLIPK++YL E LGF+R E   MV+R PALLT+S++NN  PK 
Sbjct: 153 DTITSRNTVLLVSNVERTLIPKIEYLEEGLGFTREEVAKMVVRSPALLTYSVDNNLVPKV 212

Query: 244 EYFSGEMHGKLEELKDFPQYFAFSLEKRIKPRYLETVQSGKKVPLSLMLKTTDDEFRELL 303
           E+F  EM G ++ELK FPQYF+FSLE++IKPR+    + G  +PLS MLK +D +F   L
Sbjct: 213 EFFIEEMRGDVKELKRFPQYFSFSLERKIKPRHRLLKEHGILMPLSEMLKVSDGQFNHWL 272

Query: 304 RE 305
            E
Sbjct: 273 LE 274

[Close]
BLAST of MC03g0654 vs. TAIR 10
Match: AT2G36000.1 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 171.4 bits (433), Expect = 1.1e-42
Identity = 115/315 (36.51%), Postives = 170/315 (53.97%), Query Frame = 0 

Query: 6   ATAFHSSFCFSSHKLSSSPSNSHSN-----SHQPNTLLAAKPKTLFHKHPLYTPLHS--- 65
           A +F SS   S H    S S    N     S +PNT   +   + F K P    L S   
Sbjct: 5   ALSFLSSSLPSLHHNFPSLSRLRFNNFPALSFKPNT---SSSSSSFFKSPDIPSLSSTTT 64

Query: 66  ------TLSSQVKEKILCLEIMGVDAGKALSLNPSLHSVTLESIHSVISFLQSKGIH--Q 125
                 TL S + EK++ L+ +G+D    ++ +P L S  L ++ SV+ ++ +  I+   
Sbjct: 65  TTTTTETLESSIHEKLIYLDSLGIDFLTLINRHPPLLSTALSAVESVVDYMTTPPINFTL 124

Query: 126 KDFAKIFGMCPKILTSDVKTDLIPVFNFLSEDLKIPDQSF--RRTINKCPRLLASSAKDQ 185
           +DF ++  MCP++LTS + +  IPV  FL  ++ + D  F  R+ + + PRLLA S   Q
Sbjct: 125 QDFRRLVSMCPELLTSPLTSHTIPVITFLLREVGV-DSIFDLRQALRRRPRLLACSVDHQ 184

Query: 186 LKPALFYLQRLGLRDLEALAYHDSVLLVSSVERTLIPKLKYLESLGFSRSETVGMVLRCP 245
           L+P L++LQR+G+ D     Y    LL  SV+  L+P++ Y E LGFSR     M  R P
Sbjct: 185 LRPTLYFLQRIGILDPHKHTY----LLSCSVDNKLVPRIDYFEKLGFSRRSATAMFKRFP 244

Query: 246 ALLTFSIENNFKPKFEYFSGEMHGKLEELKDFPQYFAFSLEKRIKPRYLETVQSGKKVPL 303
            L  +SI  N++PK +Y   EM   + E+ +FPQYF+FSLE RIKPR+      G + PL
Sbjct: 245 QLFNYSIAENYEPKLKYLMVEMGRDVREVLEFPQYFSFSLENRIKPRHEACAAKGVRFPL 304

[Close]
BLAST of MC03g0654 vs. TAIR 10
Match: AT2G36000.2 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 171.4 bits (433), Expect = 1.1e-42
Identity = 115/315 (36.51%), Postives = 170/315 (53.97%), Query Frame = 0 

Query: 6   ATAFHSSFCFSSHKLSSSPSNSHSN-----SHQPNTLLAAKPKTLFHKHPLYTPLHS--- 65
           A +F SS   S H    S S    N     S +PNT   +   + F K P    L S   
Sbjct: 5   ALSFLSSSLPSLHHNFPSLSRLRFNNFPALSFKPNT---SSSSSSFFKSPDIPSLSSTTT 64

Query: 66  ------TLSSQVKEKILCLEIMGVDAGKALSLNPSLHSVTLESIHSVISFLQSKGIH--Q 125
                 TL S + EK++ L+ +G+D    ++ +P L S  L ++ SV+ ++ +  I+   
Sbjct: 65  TTTTTETLESSIHEKLIYLDSLGIDFLTLINRHPPLLSTALSAVESVVDYMTTPPINFTL 124

Query: 126 KDFAKIFGMCPKILTSDVKTDLIPVFNFLSEDLKIPDQSF--RRTINKCPRLLASSAKDQ 185
           +DF ++  MCP++LTS + +  IPV  FL  ++ + D  F  R+ + + PRLLA S   Q
Sbjct: 125 QDFRRLVSMCPELLTSPLTSHTIPVITFLLREVGV-DSIFDLRQALRRRPRLLACSVDHQ 184

Query: 186 LKPALFYLQRLGLRDLEALAYHDSVLLVSSVERTLIPKLKYLESLGFSRSETVGMVLRCP 245
           L+P L++LQR+G+ D     Y    LL  SV+  L+P++ Y E LGFSR     M  R P
Sbjct: 185 LRPTLYFLQRIGILDPHKHTY----LLSCSVDNKLVPRIDYFEKLGFSRRSATAMFKRFP 244

Query: 246 ALLTFSIENNFKPKFEYFSGEMHGKLEELKDFPQYFAFSLEKRIKPRYLETVQSGKKVPL 303
            L  +SI  N++PK +Y   EM   + E+ +FPQYF+FSLE RIKPR+      G + PL
Sbjct: 245 QLFNYSIAENYEPKLKYLMVEMGRDVREVLEFPQYFSFSLENRIKPRHEACAAKGVRFPL 304

[Close]
BLAST of MC03g0654 vs. TAIR 10
Match: AT3G18870.1 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 147.9 bits (372), Expect = 1.3e-35
Identity = 91/249 (36.55%), Postives = 145/249 (58.23%), Query Frame = 0 

Query: 54  PLHSTLSSQVKEKILCLEIMGVDAGKALSLNPSL--HSVTLESIHSVISFLQSKGIHQKD 113
           PL+    +  +E +  L  +G+     +  NP L   +  L  I S ++ L+SKGI  +D
Sbjct: 27  PLYLKFQTSHRENLRHLSSLGI-----VPQNPRLAPPANDLSVILSAVNLLKSKGISDED 86

Query: 114 FAKIFGMCPKIL--TSDVKTDLIPVFNFLSEDLKIPDQSFRRTINKCPRLLASSAKDQLK 173
           F ++  +CP++   T D+ + L PVF+FL+ +L    +  R  I  CP +L S  +  L+
Sbjct: 87  FPRLVFLCPQLFSPTFDI-SKLDPVFDFLTGELGASAEESRGLIVNCPNILFSDVEYCLR 146

Query: 174 PALFYLQRLGLRDLEALAYHDSVLLVSSVERTLIPKLKYLESLGFSRSETVGMVLRCPAL 233
           P L YL+ LG+R+L   +  ++ +L + VE+ L  K+++L+S+GF   E   +  R PA+
Sbjct: 147 PTLVYLKELGVRNLNRASKTNAHVLNTRVEK-LRAKMRFLKSIGFEHEEAARVCGRIPAI 206

Query: 234 LTFSIENNFKPKFEYFSGEMHGKLEELKDFPQYFAFSLEKRIKPRYLETVQSGKKVPLSL 293
             +S+E+N +PKFE+   +M  +LEELK FPQYFAFSL KRI+PR+    +   +V LS 
Sbjct: 207 FGYSVEDNLRPKFEFLVYDMERELEELKKFPQYFAFSLGKRIRPRHWHLKKKNVRVSLSR 266

Query: 294 MLKTTDDEF 299
           ML   D +F
Sbjct: 267 MLMWGDQKF 268

The following BLAST results are available for this feature:
BLAST of MC03g0654 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q84X531.0e-5647.52Transcription termination factor MTEF1, chloroplastic OS=Arabidopsis thaliana OX... [more]
F4JVI38.7e-2430.86Transcription termination factor MTERF5, chloroplastic OS=Arabidopsis thaliana O... [more]
F4IHL34.1e-2129.12Transcription termination factor MTERF2, chloroplastic OS=Arabidopsis thaliana O... [more]
Q9ZT962.1e-1726.30Transcription termination factor MTERF4, chloroplastic OS=Arabidopsis thaliana O... [more]
Q9FM803.0e-1623.02Transcription termination factor MTERF9, chloroplastic OS=Arabidopsis thaliana O... [more]
BLAST of MC03g0654 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022139889.13.30e-215100.00transcription termination factor MTEF1, chloroplastic [Momordica charantia][more]
XP_038895185.13.32e-19390.88transcription termination factor MTEF1, chloroplastic [Benincasa hispida][more]
XP_008444692.11.28e-18889.61PREDICTED: transcription termination factor MTEF1, chloroplastic [Cucumis melo] ... [more]
XP_004154376.11.28e-18889.29transcription termination factor MTEF1, chloroplastic [Cucumis sativus] >KGN6587... [more]
KAG6583877.19.69e-18387.62Transcription termination factor MTEF1, chloroplastic, partial [Cucurbita argyro... [more]
BLAST of MC03g0654 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1CE811.60e-215100.00transcription termination factor MTEF1, chloroplastic OS=Momordica charantia OX=... [more]
A0A5D3DB896.21e-18989.61Transcription termination factor MTEF1 OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A0A0LVD46.21e-18989.29Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G535790 PE=3 SV=1[more]
A0A1S3BBS56.21e-18989.61transcription termination factor MTEF1, chloroplastic OS=Cucumis melo OX=3656 GN... [more]
A0A6J1EFX29.46e-18387.62transcription termination factor MTEF1, chloroplastic-like OS=Cucurbita moschata... [more]
BLAST of MC03g0654 vs. TAIR 10
Match NameE-valueIdentityDescription
AT2G34620.11.0e-10465.76Mitochondrial transcription termination factor family protein [more]
AT2G03050.17.3e-5847.52Mitochondrial transcription termination factor family protein [more]
AT2G36000.11.1e-4236.51Mitochondrial transcription termination factor family protein [more]
AT2G36000.21.1e-4236.51Mitochondrial transcription termination factor family protein [more]
AT3G18870.11.3e-3536.55Mitochondrial transcription termination factor family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003690Transcription termination factor, mitochondrial/chloroplasticSMARTSM00733mt_12coord: 113..145
e-value: 0.01
score: 25.0
coord: 220..252
e-value: 3.3E-4
score: 30.0
coord: 184..215
e-value: 1.1
score: 18.3
coord: 78..108
e-value: 42.0
score: 13.0
coord: 150..181
e-value: 0.0059
score: 25.8
coord: 253..286
e-value: 0.63
score: 19.1
IPR003690Transcription termination factor, mitochondrial/chloroplasticPFAMPF02536mTERFcoord: 63..182
e-value: 3.7E-11
score: 42.5
coord: 193..298
e-value: 2.9E-27
score: 95.4
IPR003690Transcription termination factor, mitochondrial/chloroplasticPANTHERPTHR13068CGI-12 PROTEIN-RELATEDcoord: 11..304
IPR038538MTERF superfamily, mitochondrial/chloroplasticGENE3D1.25.70.10Transcription termination factor 3, mitochondrialcoord: 149..304
e-value: 3.6E-31
score: 110.3
NoneNo IPR availablePANTHERPTHR13068:SF78MITOCHONDRIAL TRANSCRIPTION TERMINATION FACTOR FAMILY PROTEINcoord: 11..304

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC03g0654.1MC03g0654.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071472 cellular response to salt stress
biological_process GO:0009658 chloroplast organization
biological_process GO:0032502 developmental process
biological_process GO:0006353 DNA-templated transcription, termination
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0009507 chloroplast
cellular_component GO:0042646 plastid nucleoid
molecular_function GO:0003690 double-stranded DNA binding
molecular_function GO:0003729 mRNA binding