MC03g0568 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC03g0568
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	BOI-related E3 ubiquitin-protein ligase 1-like
Location	MC03: 12438882 .. 12439364 (+)
RNA-Seq Expression	MC03g0568
Synteny	MC03g0568

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GAAAAAGTGAGGATGGAAATGGAGGAGAGGAAGAGGAGGCAATGTTGGATGCTGGTATCGGCAATAGAAGAGAGAGTGGTGAGGAAGGTGAAGGAGAAAGAGGAGGAGATAGCGAGGATGGGGAAATTGAACTGGGTTTTGGAAGAGAGGGTGAAGCAGTTGTGTCGGGAGAATCAGCTATGGCGGGACCTGGCCGAGACCAACCAGGCCGCCGTCAACTCCCTTCGGACCAATCTCGACCACCTTCTCCACATCGCCGCCGCCGGCCGAGACCGAGACCAAGAACAAGACGACGCCCAGTCCAGCTGCGCCACGTCGCCCACCGACGACCGCCGCTCTTGCCGGAAATGTGCCGCCGGGGACTCCACGGTGTTGGTGTTGCCATGTAGGCATCTTTGCTTGTGTACAATGTGTGGGTCCACTCTTCATACTTGCCCTCTCTGTAATTCTGCCATCAATGCCACCATACATGTTAATTTCTCT

mRNA sequence

GAAAAAGTGAGGATGGAAATGGAGGAGAGGAAGAGGAGGCAATGTTGGATGCTGGTATCGGCAATAGAAGAGAGAGTGGTGAGGAAGGTGAAGGAGAAAGAGGAGGAGATAGCGAGGATGGGGAAATTGAACTGGGTTTTGGAAGAGAGGGTGAAGCAGTTGTGTCGGGAGAATCAGCTATGGCGGGACCTGGCCGAGACCAACCAGGCCGCCGTCAACTCCCTTCGGACCAATCTCGACCACCTTCTCCACATCGCCGCCGCCGGCCGAGACCGAGACCAAGAACAAGACGACGCCCAGTCCAGCTGCGCCACGTCGCCCACCGACGACCGCCGCTCTTGCCGGAAATGTGCCGCCGGGGACTCCACGGTGTTGGTGTTGCCATGTAGGCATCTTTGCTTGTGTACAATGTGTGGGTCCACTCTTCATACTTGCCCTCTCTGTAATTCTGCCATCAATGCCACCATACATGTTAATTTCTCT

Coding sequence (CDS)

GAAAAAGTGAGGATGGAAATGGAGGAGAGGAAGAGGAGGCAATGTTGGATGCTGGTATCGGCAATAGAAGAGAGAGTGGTGAGGAAGGTGAAGGAGAAAGAGGAGGAGATAGCGAGGATGGGGAAATTGAACTGGGTTTTGGAAGAGAGGGTGAAGCAGTTGTGTCGGGAGAATCAGCTATGGCGGGACCTGGCCGAGACCAACCAGGCCGCCGTCAACTCCCTTCGGACCAATCTCGACCACCTTCTCCACATCGCCGCCGCCGGCCGAGACCGAGACCAAGAACAAGACGACGCCCAGTCCAGCTGCGCCACGTCGCCCACCGACGACCGCCGCTCTTGCCGGAAATGTGCCGCCGGGGACTCCACGGTGTTGGTGTTGCCATGTAGGCATCTTTGCTTGTGTACAATGTGTGGGTCCACTCTTCATACTTGCCCTCTCTGTAATTCTGCCATCAATGCCACCATACATGTTAATTTCTCT

Protein sequence

EKVRMEMEERKRRQCWMLVSAIEERVVRKVKEKEEEIARMGKLNWVLEERVKQLCRENQLWRDLAETNQAAVNSLRTNLDHLLHIAAAGRDRDQEQDDAQSSCATSPTDDRRSCRKCAAGDSTVLVLPCRHLCLCTMCGSTLHTCPLCNSAINATIHVNFS

Homology

[Close]

BLAST of MC03g0568 vs. ExPASy Swiss-Prot
Match: Q9FHE4 (BOI-related E3 ubiquitin-protein ligase 1 OS=Arabidopsis thaliana OX=3702 GN=BRG1 PE=1 SV=1)

HSP 1 Score: 156.4 bits (394), Expect = 2.8e-37
Identity = 86/172 (50.00%), Postives = 120/172 (69.77%), Query Frame = 0


Query: 1   EKVRMEMEERKRRQCWMLVSAIEERVVRKVKEKEEEIARMGKLNWVLEERVKQLCRENQL 60
           E +R+E+E R+R Q  ML SA++  +++K+K K+EEI RMGKLNWVL+ERVK L  ENQ+
Sbjct: 122 ETLRIELEARQRTQTRMLASAVQNAILKKLKAKDEEIIRMGKLNWVLQERVKNLYVENQI 181

Query: 61  WRDLAETNQAAVNSLRTNLDHLL----HIAAAGRDRDQEQDDAQSSCATSPTDD-----R 120
           WRDLA+TN+A  N+LR+NL+ +L     + A  R   +E DDA+SSC +    D      
Sbjct: 182 WRDLAQTNEATANNLRSNLEQVLAQVDDLDAFRRPLVEEADDAESSCGSCDGGDVTAVVN 241

Query: 121 RSCRKCAAGDSTVLVLPCRHLCLCTMCGST--LHTCPLCNSAINATIHVNFS 162
             C++C    ++VLVLPCRHLCLCT+CGS+  L TCP+C+  + A++HVN S
Sbjct: 242 GGCKRCGELTASVLVLPCRHLCLCTVCGSSALLRTCPVCDMVMTASVHVNMS 293

[Close]

BLAST of MC03g0568 vs. ExPASy Swiss-Prot
Match: F4IDI6 (Probable BOI-related E3 ubiquitin-protein ligase 2 OS=Arabidopsis thaliana OX=3702 GN=BRG2 PE=1 SV=1)

HSP 1 Score: 151.0 bits (380), Expect = 1.2e-35
Identity = 86/190 (45.26%), Postives = 121/190 (63.68%), Query Frame = 0


Query: 1   EKVRMEMEERKRRQCWMLVSAIEERVVRKVKEKEEEIARMGKLNWVLEERVKQLCRENQL 60
           E+V+ E+EE+++RQ   ++ AIE+ +V++++ KEEE  R+GK+N  LEERVK L  ENQ+
Sbjct: 168 ERVKYEIEEKRKRQARTIMEAIEQGLVKRLRVKEEERERIGKVNHALEERVKSLSIENQI 227

Query: 61  WRDLAETNQAAVNSLRTNLDHLLH-----IAAAGRDRD-QEQDDAQSSCATS-------- 120
           WRDLA+TN+A  N LRTNL+H+L         AG +++  E+DDA+S C +S        
Sbjct: 228 WRDLAQTNEATANHLRTNLEHVLAQVKDVSRGAGLEKNMNEEDDAESCCGSSCGGGGEET 287

Query: 121 ---------------PTDDRRSCRKCAAGDSTVLVLPCRHLCLCTMCGSTLHTCPLCNSA 162
                              RR CR C   +S VL+LPCRHLCLC +CGS++HTCP+C S 
Sbjct: 288 VRRRVGLEREAQDKAERRRRRMCRNCGEEESCVLLLPCRHLCLCGVCGSSVHTCPICTSP 347

[Close]

BLAST of MC03g0568 vs. ExPASy Swiss-Prot
Match: O81851 (E3 ubiquitin-protein ligase BOI OS=Arabidopsis thaliana OX=3702 GN=BOI PE=1 SV=1)

HSP 1 Score: 147.5 bits (371), Expect = 1.3e-34
Identity = 81/172 (47.09%), Postives = 118/172 (68.60%), Query Frame = 0


Query: 1   EKVRMEMEERKRRQCWMLVSAIEERVVRKVKEKEEEIARMGKLNWVLEERVKQLCRENQL 60
           EK+R+E+E R++ Q  ML SA++  + +K+KEK++EI R+  LNWVL+ERVK L  ENQ+
Sbjct: 132 EKLRIEIEARQQTQTRMLASAVQNVIAKKLKEKDDEIVRIRNLNWVLQERVKSLYVENQI 191

Query: 61  WRDLAETNQAAVNSLRTNLDHLL-HIAAAGRDRDQEQDDAQSSCAT--------SPTDDR 120
           WRD+A+TN+A  N+LRTNLD +L  +          +DDA+SSC +        + T   
Sbjct: 192 WRDIAQTNEANANTLRTNLDQVLAQLETFPTASAVVEDDAESSCGSCCGDGGGEAVTAVG 251

Query: 121 RSCRKCAAGDSTVLVLPCRHLCLCTMCGST--LHTCPLCNSAINATIHVNFS 162
             C++C   +++VLVLPCRHLCLCT+CG +  L TCP+C+  +NA++HVN S
Sbjct: 252 GGCKRCGEREASVLVLPCRHLCLCTVCGGSALLRTCPVCDMVMNASVHVNMS 303

[Close]

BLAST of MC03g0568 vs. ExPASy Swiss-Prot
Match: Q9LDD1 (Probable BOI-related E3 ubiquitin-protein ligase 3 OS=Arabidopsis thaliana OX=3702 GN=BRG3 PE=1 SV=1)

HSP 1 Score: 144.8 bits (364), Expect = 8.3e-34
Identity = 80/187 (42.78%), Postives = 122/187 (65.24%), Query Frame = 0


Query: 1   EKVRMEMEERKRRQCWMLVSAIEERVVRKVKEKEEEIARMGKLNWVLEERVKQLCRENQL 60
           E++RME+EE+++ Q   +V A+E+ +++ ++ K++EI  +GKLN  LEE+VK LC ENQ+
Sbjct: 150 ERMRMEIEEKRKTQGRRIVEAVEQGLMKTLRAKDDEINHIGKLNLFLEEKVKSLCVENQI 209

Query: 61  WRDLAETNQAAVNSLRTNLDHLLHIAAAGRDRDQE----QDDAQSSCATSPTDDRRS--- 120
           WRD+A++N+A VN+LR+NL  +L  AA  R+R +E     DDAQS C ++   D      
Sbjct: 210 WRDVAQSNEATVNALRSNLQQVL--AAVERNRWEEPPTVADDAQSCCGSNDEGDSEEERW 269

Query: 121 -------------------CRKCAAGDSTVLVLPCRHLCLCTMCGSTLHTCPLCNSAINA 162
                              CR C  G+++VL+LPCRH+CLC++CGS+L+TCP+C S   A
Sbjct: 270 KLAGEAQDTKKMCRVGMSMCRSCGKGEASVLLLPCRHMCLCSVCGSSLNTCPICKSPKTA 329

[Close]

BLAST of MC03g0568 vs. NCBI nr
Match: XP_022142559.1 (BOI-related E3 ubiquitin-protein ligase 1-like [Momordica charantia])

HSP 1 Score: 315 bits (806), Expect = 1.68e-106
Identity = 161/161 (100.00%), Postives = 161/161 (100.00%), Query Frame = 0


Query: 1   EKVRMEMEERKRRQCWMLVSAIEERVVRKVKEKEEEIARMGKLNWVLEERVKQLCRENQL 60
           EKVRMEMEERKRRQCWMLVSAIEERVVRKVKEKEEEIARMGKLNWVLEERVKQLCRENQL
Sbjct: 104 EKVRMEMEERKRRQCWMLVSAIEERVVRKVKEKEEEIARMGKLNWVLEERVKQLCRENQL 163

Query: 61  WRDLAETNQAAVNSLRTNLDHLLHIAAAGRDRDQEQDDAQSSCATSPTDDRRSCRKCAAG 120
           WRDLAETNQAAVNSLRTNLDHLLHIAAAGRDRDQEQDDAQSSCATSPTDDRRSCRKCAAG
Sbjct: 164 WRDLAETNQAAVNSLRTNLDHLLHIAAAGRDRDQEQDDAQSSCATSPTDDRRSCRKCAAG 223

Query: 121 DSTVLVLPCRHLCLCTMCGSTLHTCPLCNSAINATIHVNFS 161
           DSTVLVLPCRHLCLCTMCGSTLHTCPLCNSAINATIHVNFS
Sbjct: 224 DSTVLVLPCRHLCLCTMCGSTLHTCPLCNSAINATIHVNFS 264

[Close]

BLAST of MC03g0568 vs. NCBI nr
Match: XP_022994865.1 (BOI-related E3 ubiquitin-protein ligase 1-like [Cucurbita maxima])

HSP 1 Score: 206 bits (525), Expect = 6.44e-64
Identity = 113/162 (69.75%), Postives = 132/162 (81.48%), Query Frame = 0


Query: 1   EKVRMEMEERKRRQCWMLVSAIEERVVRKVKEKEEEIARMGKLNWVLEERVKQLCRENQL 60
           EKV MEMEERK+RQ  MLVSAIE+RVV+K+KEKEEEI RMGKLNWVL+ERVK L  ENQ 
Sbjct: 96  EKVMMEMEERKQRQSRMLVSAIEDRVVKKLKEKEEEIERMGKLNWVLQERVKHLSLENQQ 155

Query: 61  WRDLAETNQAAVNSLRTNLDHLLHIAAAGRDRDQEQDDAQSSCATSPTDDRRS-CRKCAA 120
           WR+LAETN+A +NSLRTNL+ +L +A AG D     DDA+SSC +S +      CRKC A
Sbjct: 156 WRNLAETNEATLNSLRTNLEQVL-MANAGEDAAASADDAESSCGSSYSGGGGGRCRKCGA 215

Query: 121 GDSTVLVLPCRHLCLCTMCGSTLHTCPLCNSAINATIHVNFS 161
            +S VLVLPCRHLCLCT+CGS LHTCP+CNSAINA++HVNFS
Sbjct: 216 AESRVLVLPCRHLCLCTICGSRLHTCPVCNSAINASVHVNFS 256

[Close]

BLAST of MC03g0568 vs. NCBI nr
Match: XP_023541940.1 (BOI-related E3 ubiquitin-protein ligase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 206 bits (525), Expect = 6.44e-64
Identity = 113/162 (69.75%), Postives = 133/162 (82.10%), Query Frame = 0


Query: 1   EKVRMEMEERKRRQCWMLVSAIEERVVRKVKEKEEEIARMGKLNWVLEERVKQLCRENQL 60
           EKV MEMEERK+RQ  MLVSAIE+RVV+K+KEKEEEI RMGKLNWVL+ERVK L  ENQ 
Sbjct: 96  EKVMMEMEERKQRQSRMLVSAIEDRVVKKLKEKEEEIERMGKLNWVLQERVKHLSLENQQ 155

Query: 61  WRDLAETNQAAVNSLRTNLDHLLHIAAAGRDRDQEQDDAQSSCATSPTDDRRS-CRKCAA 120
           WR+LAETN+A +NSLRTNL+ +L +A AG +     DDA+SSC +S +      CRKC A
Sbjct: 156 WRNLAETNEATLNSLRTNLEQVL-MANAGEEAAAVADDAESSCGSSYSGGGGGRCRKCGA 215

Query: 121 GDSTVLVLPCRHLCLCTMCGSTLHTCPLCNSAINATIHVNFS 161
            +STVLVLPCRHLCLCT+CGS LHTCP+CNSAINA++HVNFS
Sbjct: 216 AESTVLVLPCRHLCLCTICGSRLHTCPVCNSAINASVHVNFS 256

[Close]

BLAST of MC03g0568 vs. NCBI nr
Match: XP_023519053.1 (BOI-related E3 ubiquitin-protein ligase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 207 bits (527), Expect = 8.44e-64
Identity = 113/169 (66.86%), Postives = 136/169 (80.47%), Query Frame = 0


Query: 1   EKVRMEMEERKRRQCWMLVSAIEERVVRKVKEKEEEIARMGKLNWVLEERVKQLCRENQL 60
           EKVRM ME RK+R+  MLV AIEER+V+K+KEK+EEI RMGKLNWVL+ERVK+LC ENQL
Sbjct: 123 EKVRMAMEIRKKRESGMLVRAIEERLVKKLKEKDEEIERMGKLNWVLQERVKRLCMENQL 182

Query: 61  WRDLAETNQAAVNSLRTNLDHLLHIAAAGRDRDQEQDDAQSSCATSP----TDDR----R 120
           WRDLAETN+A VNSLRTNL+ L+   +A  D     +DA+SSC ++     T++     R
Sbjct: 183 WRDLAETNEATVNSLRTNLERLI---SAANDTATAANDAESSCGSNSGCGITEEESGGGR 242

Query: 121 SCRKCAAGDSTVLVLPCRHLCLCTMCGSTLHTCPLCNSAINATIHVNFS 161
            CRKC AG+S VLVLPCRHLCLCTMCGSTLHTCP+C+SAINA++HVNFS
Sbjct: 243 RCRKCMAGESRVLVLPCRHLCLCTMCGSTLHTCPVCDSAINASVHVNFS 288

[Close]

BLAST of MC03g0568 vs. NCBI nr
Match: XP_022927535.1 (BOI-related E3 ubiquitin-protein ligase 1-like [Cucurbita moschata] >KAG6583800.1 BOI-related E3 ubiquitin-protein ligase 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7019425.1 BOI-related E3 ubiquitin-protein ligase 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 207 bits (527), Expect = 8.44e-64
Identity = 113/169 (66.86%), Postives = 136/169 (80.47%), Query Frame = 0


Query: 1   EKVRMEMEERKRRQCWMLVSAIEERVVRKVKEKEEEIARMGKLNWVLEERVKQLCRENQL 60
           EKVRM ME RK+R+  MLV AIEER+V+K+KEK+EEI RMGKLNWVL+ERVK+LC ENQL
Sbjct: 123 EKVRMAMEIRKKRESGMLVRAIEERLVKKLKEKDEEIERMGKLNWVLQERVKRLCMENQL 182

Query: 61  WRDLAETNQAAVNSLRTNLDHLLHIAAAGRDRDQEQDDAQSSCATSP----TDDR----R 120
           WRDLAETN+A VNSLRTNL+ L+   +A  D     +DA+SSC ++     T++     R
Sbjct: 183 WRDLAETNEATVNSLRTNLERLI---SAANDTAAAANDAESSCGSNSGCGITEEESGGGR 242

Query: 121 SCRKCAAGDSTVLVLPCRHLCLCTMCGSTLHTCPLCNSAINATIHVNFS 161
            CRKC AG+S VLVLPCRHLCLCTMCGSTLHTCP+C+SAINA++HVNFS
Sbjct: 243 RCRKCMAGESRVLVLPCRHLCLCTMCGSTLHTCPVCDSAINASVHVNFS 288

[Close]

BLAST of MC03g0568 vs. ExPASy TrEMBL
Match: A0A6J1CLU6 (BOI-related E3 ubiquitin-protein ligase 1-like OS=Momordica charantia OX=3673 GN=LOC111012642 PE=4 SV=1)

HSP 1 Score: 315 bits (806), Expect = 8.11e-107
Identity = 161/161 (100.00%), Postives = 161/161 (100.00%), Query Frame = 0


Query: 1   EKVRMEMEERKRRQCWMLVSAIEERVVRKVKEKEEEIARMGKLNWVLEERVKQLCRENQL 60
           EKVRMEMEERKRRQCWMLVSAIEERVVRKVKEKEEEIARMGKLNWVLEERVKQLCRENQL
Sbjct: 104 EKVRMEMEERKRRQCWMLVSAIEERVVRKVKEKEEEIARMGKLNWVLEERVKQLCRENQL 163

Query: 61  WRDLAETNQAAVNSLRTNLDHLLHIAAAGRDRDQEQDDAQSSCATSPTDDRRSCRKCAAG 120
           WRDLAETNQAAVNSLRTNLDHLLHIAAAGRDRDQEQDDAQSSCATSPTDDRRSCRKCAAG
Sbjct: 164 WRDLAETNQAAVNSLRTNLDHLLHIAAAGRDRDQEQDDAQSSCATSPTDDRRSCRKCAAG 223

Query: 121 DSTVLVLPCRHLCLCTMCGSTLHTCPLCNSAINATIHVNFS 161
           DSTVLVLPCRHLCLCTMCGSTLHTCPLCNSAINATIHVNFS
Sbjct: 224 DSTVLVLPCRHLCLCTMCGSTLHTCPLCNSAINATIHVNFS 264

[Close]

BLAST of MC03g0568 vs. ExPASy TrEMBL
Match: A0A6J1K2I4 (BOI-related E3 ubiquitin-protein ligase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111490463 PE=4 SV=1)

HSP 1 Score: 206 bits (525), Expect = 3.12e-64
Identity = 113/162 (69.75%), Postives = 132/162 (81.48%), Query Frame = 0


Query: 1   EKVRMEMEERKRRQCWMLVSAIEERVVRKVKEKEEEIARMGKLNWVLEERVKQLCRENQL 60
           EKV MEMEERK+RQ  MLVSAIE+RVV+K+KEKEEEI RMGKLNWVL+ERVK L  ENQ 
Sbjct: 96  EKVMMEMEERKQRQSRMLVSAIEDRVVKKLKEKEEEIERMGKLNWVLQERVKHLSLENQQ 155

Query: 61  WRDLAETNQAAVNSLRTNLDHLLHIAAAGRDRDQEQDDAQSSCATSPTDDRRS-CRKCAA 120
           WR+LAETN+A +NSLRTNL+ +L +A AG D     DDA+SSC +S +      CRKC A
Sbjct: 156 WRNLAETNEATLNSLRTNLEQVL-MANAGEDAAASADDAESSCGSSYSGGGGGRCRKCGA 215

Query: 121 GDSTVLVLPCRHLCLCTMCGSTLHTCPLCNSAINATIHVNFS 161
            +S VLVLPCRHLCLCT+CGS LHTCP+CNSAINA++HVNFS
Sbjct: 216 AESRVLVLPCRHLCLCTICGSRLHTCPVCNSAINASVHVNFS 256

[Close]

BLAST of MC03g0568 vs. ExPASy TrEMBL
Match: A0A6J1EP88 (BOI-related E3 ubiquitin-protein ligase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111434333 PE=4 SV=1)

HSP 1 Score: 207 bits (527), Expect = 4.09e-64
Identity = 113/169 (66.86%), Postives = 136/169 (80.47%), Query Frame = 0


Query: 1   EKVRMEMEERKRRQCWMLVSAIEERVVRKVKEKEEEIARMGKLNWVLEERVKQLCRENQL 60
           EKVRM ME RK+R+  MLV AIEER+V+K+KEK+EEI RMGKLNWVL+ERVK+LC ENQL
Sbjct: 123 EKVRMAMEIRKKRESGMLVRAIEERLVKKLKEKDEEIERMGKLNWVLQERVKRLCMENQL 182

Query: 61  WRDLAETNQAAVNSLRTNLDHLLHIAAAGRDRDQEQDDAQSSCATSP----TDDR----R 120
           WRDLAETN+A VNSLRTNL+ L+   +A  D     +DA+SSC ++     T++     R
Sbjct: 183 WRDLAETNEATVNSLRTNLERLI---SAANDTAAAANDAESSCGSNSGCGITEEESGGGR 242

Query: 121 SCRKCAAGDSTVLVLPCRHLCLCTMCGSTLHTCPLCNSAINATIHVNFS 161
            CRKC AG+S VLVLPCRHLCLCTMCGSTLHTCP+C+SAINA++HVNFS
Sbjct: 243 RCRKCMAGESRVLVLPCRHLCLCTMCGSTLHTCPVCDSAINASVHVNFS 288

[Close]

BLAST of MC03g0568 vs. ExPASy TrEMBL
Match: A0A6J1GUS0 (BOI-related E3 ubiquitin-protein ligase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111457113 PE=4 SV=1)

HSP 1 Score: 204 bits (519), Expect = 2.53e-63
Identity = 112/162 (69.14%), Postives = 132/162 (81.48%), Query Frame = 0


Query: 1   EKVRMEMEERKRRQCWMLVSAIEERVVRKVKEKEEEIARMGKLNWVLEERVKQLCRENQL 60
           EKV MEMEERK+RQ  MLVSAIE+RVV+K+KEKEEEI RMGKLNWVL+ERVK L  ENQ 
Sbjct: 96  EKVMMEMEERKQRQSRMLVSAIEDRVVKKLKEKEEEIERMGKLNWVLQERVKHLSLENQQ 155

Query: 61  WRDLAETNQAAVNSLRTNLDHLLHIAAAGRDRDQEQDDAQSSCATSPTDDRRS-CRKCAA 120
           WR+LAETN+A +NSLRTNL+ +L +A AG +     DDA+SSC +S +      CRKC A
Sbjct: 156 WRNLAETNEATLNSLRTNLEQVL-MANAGEEAAAVADDAESSCGSSYSGGGGGRCRKCGA 215

Query: 121 GDSTVLVLPCRHLCLCTMCGSTLHTCPLCNSAINATIHVNFS 161
            +S VLVLPCRHLCLCT+CGS LHTCP+CNSAINA++HVNFS
Sbjct: 216 AESRVLVLPCRHLCLCTICGSRLHTCPVCNSAINASVHVNFS 256

[Close]

BLAST of MC03g0568 vs. ExPASy TrEMBL
Match: A0A6J1KKD3 (BOI-related E3 ubiquitin-protein ligase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111495377 PE=4 SV=1)

HSP 1 Score: 204 bits (520), Expect = 5.27e-63
Identity = 111/170 (65.29%), Postives = 132/170 (77.65%), Query Frame = 0


Query: 1   EKVRMEMEERKRRQCWMLVSAIEERVVRKVKEKEEEIARMGKLNWVLEERVKQLCRENQL 60
           EKVRM ME RK+R+  MLV AIEER+V+K+KEK+EEI RMGKLNWVL+ERVK+LC ENQL
Sbjct: 123 EKVRMAMEVRKKRESGMLVRAIEERLVKKLKEKDEEIERMGKLNWVLQERVKRLCMENQL 182

Query: 61  WRDLAETNQAAVNSLRTNLDHLL----HIAAAGRDRDQEQDDAQSSCATSPTDDRRS--- 120
           WRDLAETN+A VNSLRTNL+ L+      AAAG D +      + +  T   ++  S   
Sbjct: 183 WRDLAETNEATVNSLRTNLERLISAANETAAAGNDAESSCGSNRGNGMTEEEEEEESGGG 242

Query: 121 --CRKCAAGDSTVLVLPCRHLCLCTMCGSTLHTCPLCNSAINATIHVNFS 161
             CRKC AG+S VLVLPCRHLCLCTMCGSTLHTCP+C+SAINA++HVNFS
Sbjct: 243 RRCRKCMAGESRVLVLPCRHLCLCTMCGSTLHTCPVCDSAINASVHVNFS 292

[Close]

BLAST of MC03g0568 vs. TAIR 10
Match: AT5G45100.1 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 156.4 bits (394), Expect = 2.0e-38
Identity = 86/172 (50.00%), Postives = 120/172 (69.77%), Query Frame = 0


Query: 1   EKVRMEMEERKRRQCWMLVSAIEERVVRKVKEKEEEIARMGKLNWVLEERVKQLCRENQL 60
           E +R+E+E R+R Q  ML SA++  +++K+K K+EEI RMGKLNWVL+ERVK L  ENQ+
Sbjct: 122 ETLRIELEARQRTQTRMLASAVQNAILKKLKAKDEEIIRMGKLNWVLQERVKNLYVENQI 181

Query: 61  WRDLAETNQAAVNSLRTNLDHLL----HIAAAGRDRDQEQDDAQSSCATSPTDD-----R 120
           WRDLA+TN+A  N+LR+NL+ +L     + A  R   +E DDA+SSC +    D      
Sbjct: 182 WRDLAQTNEATANNLRSNLEQVLAQVDDLDAFRRPLVEEADDAESSCGSCDGGDVTAVVN 241

Query: 121 RSCRKCAAGDSTVLVLPCRHLCLCTMCGST--LHTCPLCNSAINATIHVNFS 162
             C++C    ++VLVLPCRHLCLCT+CGS+  L TCP+C+  + A++HVN S
Sbjct: 242 GGCKRCGELTASVLVLPCRHLCLCTVCGSSALLRTCPVCDMVMTASVHVNMS 293

[Close]

BLAST of MC03g0568 vs. TAIR 10
Match: AT5G45100.2 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 156.4 bits (394), Expect = 2.0e-38
Identity = 86/172 (50.00%), Postives = 120/172 (69.77%), Query Frame = 0


Query: 1   EKVRMEMEERKRRQCWMLVSAIEERVVRKVKEKEEEIARMGKLNWVLEERVKQLCRENQL 60
           E +R+E+E R+R Q  ML SA++  +++K+K K+EEI RMGKLNWVL+ERVK L  ENQ+
Sbjct: 95  ETLRIELEARQRTQTRMLASAVQNAILKKLKAKDEEIIRMGKLNWVLQERVKNLYVENQI 154

Query: 61  WRDLAETNQAAVNSLRTNLDHLL----HIAAAGRDRDQEQDDAQSSCATSPTDD-----R 120
           WRDLA+TN+A  N+LR+NL+ +L     + A  R   +E DDA+SSC +    D      
Sbjct: 155 WRDLAQTNEATANNLRSNLEQVLAQVDDLDAFRRPLVEEADDAESSCGSCDGGDVTAVVN 214

Query: 121 RSCRKCAAGDSTVLVLPCRHLCLCTMCGST--LHTCPLCNSAINATIHVNFS 162
             C++C    ++VLVLPCRHLCLCT+CGS+  L TCP+C+  + A++HVN S
Sbjct: 215 GGCKRCGELTASVLVLPCRHLCLCTVCGSSALLRTCPVCDMVMTASVHVNMS 266

[Close]

BLAST of MC03g0568 vs. TAIR 10
Match: AT1G79110.1 (zinc ion binding )

HSP 1 Score: 151.0 bits (380), Expect = 8.3e-37
Identity = 86/190 (45.26%), Postives = 121/190 (63.68%), Query Frame = 0


Query: 1   EKVRMEMEERKRRQCWMLVSAIEERVVRKVKEKEEEIARMGKLNWVLEERVKQLCRENQL 60
           E+V+ E+EE+++RQ   ++ AIE+ +V++++ KEEE  R+GK+N  LEERVK L  ENQ+
Sbjct: 168 ERVKYEIEEKRKRQARTIMEAIEQGLVKRLRVKEEERERIGKVNHALEERVKSLSIENQI 227

Query: 61  WRDLAETNQAAVNSLRTNLDHLLH-----IAAAGRDRD-QEQDDAQSSCATS-------- 120
           WRDLA+TN+A  N LRTNL+H+L         AG +++  E+DDA+S C +S        
Sbjct: 228 WRDLAQTNEATANHLRTNLEHVLAQVKDVSRGAGLEKNMNEEDDAESCCGSSCGGGGEET 287

Query: 121 ---------------PTDDRRSCRKCAAGDSTVLVLPCRHLCLCTMCGSTLHTCPLCNSA 162
                              RR CR C   +S VL+LPCRHLCLC +CGS++HTCP+C S 
Sbjct: 288 VRRRVGLEREAQDKAERRRRRMCRNCGEEESCVLLLPCRHLCLCGVCGSSVHTCPICTSP 347

[Close]

BLAST of MC03g0568 vs. TAIR 10
Match: AT1G79110.2 (zinc ion binding )

HSP 1 Score: 151.0 bits (380), Expect = 8.3e-37
Identity = 86/190 (45.26%), Postives = 121/190 (63.68%), Query Frame = 0


Query: 1   EKVRMEMEERKRRQCWMLVSAIEERVVRKVKEKEEEIARMGKLNWVLEERVKQLCRENQL 60
           E+V+ E+EE+++RQ   ++ AIE+ +V++++ KEEE  R+GK+N  LEERVK L  ENQ+
Sbjct: 165 ERVKYEIEEKRKRQARTIMEAIEQGLVKRLRVKEEERERIGKVNHALEERVKSLSIENQI 224

Query: 61  WRDLAETNQAAVNSLRTNLDHLLH-----IAAAGRDRD-QEQDDAQSSCATS-------- 120
           WRDLA+TN+A  N LRTNL+H+L         AG +++  E+DDA+S C +S        
Sbjct: 225 WRDLAQTNEATANHLRTNLEHVLAQVKDVSRGAGLEKNMNEEDDAESCCGSSCGGGGEET 284

Query: 121 ---------------PTDDRRSCRKCAAGDSTVLVLPCRHLCLCTMCGSTLHTCPLCNSA 162
                              RR CR C   +S VL+LPCRHLCLC +CGS++HTCP+C S 
Sbjct: 285 VRRRVGLEREAQDKAERRRRRMCRNCGEEESCVLLLPCRHLCLCGVCGSSVHTCPICTSP 344

[Close]

BLAST of MC03g0568 vs. TAIR 10
Match: AT4G19700.1 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 147.5 bits (371), Expect = 9.1e-36
Identity = 81/172 (47.09%), Postives = 118/172 (68.60%), Query Frame = 0


Query: 1   EKVRMEMEERKRRQCWMLVSAIEERVVRKVKEKEEEIARMGKLNWVLEERVKQLCRENQL 60
           EK+R+E+E R++ Q  ML SA++  + +K+KEK++EI R+  LNWVL+ERVK L  ENQ+
Sbjct: 132 EKLRIEIEARQQTQTRMLASAVQNVIAKKLKEKDDEIVRIRNLNWVLQERVKSLYVENQI 191

Query: 61  WRDLAETNQAAVNSLRTNLDHLL-HIAAAGRDRDQEQDDAQSSCAT--------SPTDDR 120
           WRD+A+TN+A  N+LRTNLD +L  +          +DDA+SSC +        + T   
Sbjct: 192 WRDIAQTNEANANTLRTNLDQVLAQLETFPTASAVVEDDAESSCGSCCGDGGGEAVTAVG 251

Query: 121 RSCRKCAAGDSTVLVLPCRHLCLCTMCGST--LHTCPLCNSAINATIHVNFS 162
             C++C   +++VLVLPCRHLCLCT+CG +  L TCP+C+  +NA++HVN S
Sbjct: 252 GGCKRCGEREASVLVLPCRHLCLCTVCGGSALLRTCPVCDMVMNASVHVNMS 303

The following BLAST results are available for this feature:

BLAST of MC03g0568 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FHE4	2.8e-37	50.00	BOI-related E3 ubiquitin-protein ligase 1 OS=Arabidopsis thaliana OX=3702 GN=BRG...	[more]
F4IDI6	1.2e-35	45.26	Probable BOI-related E3 ubiquitin-protein ligase 2 OS=Arabidopsis thaliana OX=37...	[more]
O81851	1.3e-34	47.09	E3 ubiquitin-protein ligase BOI OS=Arabidopsis thaliana OX=3702 GN=BOI PE=1 SV=1	[more]
Q9LDD1	8.3e-34	42.78	Probable BOI-related E3 ubiquitin-protein ligase 3 OS=Arabidopsis thaliana OX=37...	[more]

BLAST of MC03g0568 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022142559.1	1.68e-106	100.00	BOI-related E3 ubiquitin-protein ligase 1-like [Momordica charantia]	[more]
XP_022994865.1	6.44e-64	69.75	BOI-related E3 ubiquitin-protein ligase 1-like [Cucurbita maxima]	[more]
XP_023541940.1	6.44e-64	69.75	BOI-related E3 ubiquitin-protein ligase 1-like [Cucurbita pepo subsp. pepo]	[more]
XP_023519053.1	8.44e-64	66.86	BOI-related E3 ubiquitin-protein ligase 1-like [Cucurbita pepo subsp. pepo]	[more]
XP_022927535.1	8.44e-64	66.86	BOI-related E3 ubiquitin-protein ligase 1-like [Cucurbita moschata] >KAG6583800....	[more]

BLAST of MC03g0568 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CLU6	8.11e-107	100.00	BOI-related E3 ubiquitin-protein ligase 1-like OS=Momordica charantia OX=3673 GN...	[more]
A0A6J1K2I4	3.12e-64	69.75	BOI-related E3 ubiquitin-protein ligase 1-like OS=Cucurbita maxima OX=3661 GN=LO...	[more]
A0A6J1EP88	4.09e-64	66.86	BOI-related E3 ubiquitin-protein ligase 1-like OS=Cucurbita moschata OX=3662 GN=...	[more]
A0A6J1GUS0	2.53e-63	69.14	BOI-related E3 ubiquitin-protein ligase 1-like OS=Cucurbita moschata OX=3662 GN=...	[more]
A0A6J1KKD3	5.27e-63	65.29	BOI-related E3 ubiquitin-protein ligase 1-like OS=Cucurbita maxima OX=3661 GN=LO...	[more]

BLAST of MC03g0568 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G45100.1	2.0e-38	50.00	SBP (S-ribonuclease binding protein) family protein	[more]
AT5G45100.2	2.0e-38	50.00	SBP (S-ribonuclease binding protein) family protein	[more]
AT1G79110.1	8.3e-37	45.26	zinc ion binding	[more]
AT1G79110.2	8.3e-37	45.26	zinc ion binding	[more]
AT4G19700.1	9.1e-36	47.09	SBP (S-ribonuclease binding protein) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR017066	E3 ubiquitin-protein ligase BOI-like	PIRSF	PIRSF036836	SBP1_RNase_bind	coord: 1..161 e-value: 3.5E-46 score: 156.4
IPR017066	E3 ubiquitin-protein ligase BOI-like	PANTHER	PTHR42647	SBP (S-RIBONUCLEASE BINDING PROTEIN) FAMILY PROTEIN	coord: 1..160
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 88..160 e-value: 1.1E-12 score: 49.2
None	No IPR available	PFAM	PF13920	zf-C3HC4_3	coord: 111..153 e-value: 4.0E-9 score: 36.2
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 90..111
None	No IPR available	PANTHER	PTHR42647:SF12	BOI-RELATED E3 UBIQUITIN-PROTEIN LIGASE 2-RELATED	coord: 1..160
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 114..149 score: 8.963311

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC03g0568.1	MC03g0568.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043067	regulation of programmed cell death
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0004842	ubiquitin-protein transferase activity