MC03g0451 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC03g0451
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionPoly(A)-specific ribonuclease
LocationMC03: 11377269 .. 11378102 (-)
RNA-Seq ExpressionMC03g0451
SyntenyMC03g0451
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTTGTCCAGGATCTGAAGAACAACAGTAAACCGATCCAAATCCGTGAAGTTTGGGCGTCGAATCTCGAATCGGAGTTCGAGTTGATTCGCGACTTGATCGATCAGTTTCCTTGCGTTTCCATGGACACGGAGTTTCCCGGAGTCGTTTTCACCGATCCTGCGAAGCCGTACCACCGCCAGAATCGGCCGTCGGACCACTACCGCCTCCTCAAATCCAACGTCGACGCTCTGAATCTGATCCAGATCGGAATCACTCTCTCCGACTCCGACGGCAACCTCCCGGACCTCGGCACCGGCGGCAGCCGCTTCATCTGGGAGTTCAACTTCATGGACTTCGACGTGGCGCGTGACGTCCACGCGCCGGACTCGATCGAGCTTCTGCGGCGGCAGGGGATCGACTTCGACCGGAACCGAGTGGAAGGTATCGACTCTTGCCGGTTCGCCGAGTTGATGATGTCCTCGGGACTCGTCTGCAACGACTCAGTGAGTTGGGTGACGTTTCACAGCGCGTACGATTTCGGGTACCTGGTCAAGGTTTTGACTCGGCGGAGCCTCCCGAGTCGGCTGGAGGAATTCCTCGGCGTTCTCAGGGTTTTCTTCGGCGATCAGGTGTACGATGTGAAGTACATGATGCGGTTCTGTAAGAGCCTCTACGGCGGTTTGGACCGGATAGCGAAGATCTTAAACGTGAGCCGGGCGGTTGGAAAGTGCCACCAGGCCGGTTCGGATAGCCTGCTGACGTGGCATGCGTTTCAGAAAATGAGGGACACGTATTTTGTACAGAATGGGCTGGAGAAACATGCCGGCGTGTTGTATGGATTGGAAGTTTTG

mRNA sequence

ATGGTTGTCCAGGATCTGAAGAACAACAGTAAACCGATCCAAATCCGTGAAGTTTGGGCGTCGAATCTCGAATCGGAGTTCGAGTTGATTCGCGACTTGATCGATCAGTTTCCTTGCGTTTCCATGGACACGGAGTTTCCCGGAGTCGTTTTCACCGATCCTGCGAAGCCGTACCACCGCCAGAATCGGCCGTCGGACCACTACCGCCTCCTCAAATCCAACGTCGACGCTCTGAATCTGATCCAGATCGGAATCACTCTCTCCGACTCCGACGGCAACCTCCCGGACCTCGGCACCGGCGGCAGCCGCTTCATCTGGGAGTTCAACTTCATGGACTTCGACGTGGCGCGTGACGTCCACGCGCCGGACTCGATCGAGCTTCTGCGGCGGCAGGGGATCGACTTCGACCGGAACCGAGTGGAAGGTATCGACTCTTGCCGGTTCGCCGAGTTGATGATGTCCTCGGGACTCGTCTGCAACGACTCAGTGAGTTGGGTGACGTTTCACAGCGCGTACGATTTCGGGTACCTGGTCAAGGTTTTGACTCGGCGGAGCCTCCCGAGTCGGCTGGAGGAATTCCTCGGCGTTCTCAGGGTTTTCTTCGGCGATCAGGTGTACGATGTGAAGTACATGATGCGGTTCTGTAAGAGCCTCTACGGCGGTTTGGACCGGATAGCGAAGATCTTAAACGTGAGCCGGGCGGTTGGAAAGTGCCACCAGGCCGGTTCGGATAGCCTGCTGACGTGGCATGCGTTTCAGAAAATGAGGGACACGTATTTTGTACAGAATGGGCTGGAGAAACATGCCGGCGTGTTGTATGGATTGGAAGTTTTG

Coding sequence (CDS)

ATGGTTGTCCAGGATCTGAAGAACAACAGTAAACCGATCCAAATCCGTGAAGTTTGGGCGTCGAATCTCGAATCGGAGTTCGAGTTGATTCGCGACTTGATCGATCAGTTTCCTTGCGTTTCCATGGACACGGAGTTTCCCGGAGTCGTTTTCACCGATCCTGCGAAGCCGTACCACCGCCAGAATCGGCCGTCGGACCACTACCGCCTCCTCAAATCCAACGTCGACGCTCTGAATCTGATCCAGATCGGAATCACTCTCTCCGACTCCGACGGCAACCTCCCGGACCTCGGCACCGGCGGCAGCCGCTTCATCTGGGAGTTCAACTTCATGGACTTCGACGTGGCGCGTGACGTCCACGCGCCGGACTCGATCGAGCTTCTGCGGCGGCAGGGGATCGACTTCGACCGGAACCGAGTGGAAGGTATCGACTCTTGCCGGTTCGCCGAGTTGATGATGTCCTCGGGACTCGTCTGCAACGACTCAGTGAGTTGGGTGACGTTTCACAGCGCGTACGATTTCGGGTACCTGGTCAAGGTTTTGACTCGGCGGAGCCTCCCGAGTCGGCTGGAGGAATTCCTCGGCGTTCTCAGGGTTTTCTTCGGCGATCAGGTGTACGATGTGAAGTACATGATGCGGTTCTGTAAGAGCCTCTACGGCGGTTTGGACCGGATAGCGAAGATCTTAAACGTGAGCCGGGCGGTTGGAAAGTGCCACCAGGCCGGTTCGGATAGCCTGCTGACGTGGCATGCGTTTCAGAAAATGAGGGACACGTATTTTGTACAGAATGGGCTGGAGAAACATGCCGGCGTGTTGTATGGATTGGAAGTTTTG

Protein sequence

MVVQDLKNNSKPIQIREVWASNLESEFELIRDLIDQFPCVSMDTEFPGVVFTDPAKPYHRQNRPSDHYRLLKSNVDALNLIQIGITLSDSDGNLPDLGTGGSRFIWEFNFMDFDVARDVHAPDSIELLRRQGIDFDRNRVEGIDSCRFAELMMSSGLVCNDSVSWVTFHSAYDFGYLVKVLTRRSLPSRLEEFLGVLRVFFGDQVYDVKYMMRFCKSLYGGLDRIAKILNVSRAVGKCHQAGSDSLLTWHAFQKMRDTYFVQNGLEKHAGVLYGLEVL
Homology
BLAST of MC03g0451 vs. ExPASy Swiss-Prot
Match: Q9FMS6 (Probable CCR4-associated factor 1 homolog 11 OS=Arabidopsis thaliana OX=3702 GN=CAF1-11 PE=2 SV=1)

HSP 1 Score: 402.5 bits (1033), Expect = 3.8e-111
Identity = 195/270 (72.22%), Postives = 227/270 (84.07%), Query Frame = 0

Query: 10  SKPIQIREVWASNLESEFELIRDLIDQFPCVSMDTEFPGVVFTDPAKPYHRQNRPSDHYR 69
           S  I IR+VWA NLESEF+LIR +++ +P +SMDTEFPGV++        R N P+  Y 
Sbjct: 9   SDVIVIRDVWAYNLESEFDLIRGIVEDYPFISMDTEFPGVIYKADLDVLRRGN-PNYLYN 68

Query: 70  LLKSNVDALNLIQIGITLSDSDGNLPDL-GTGGSRFIWEFNFMDFDVARDVHAPDSIELL 129
           LLKSNVDAL+LIQ+G+TLSD+DGNLPDL G    R+IWEFNF DFDV RD HAPDSIELL
Sbjct: 69  LLKSNVDALSLIQVGLTLSDADGNLPDLGGQKNRRYIWEFNFRDFDVERDPHAPDSIELL 128

Query: 130 RRQGIDFDRNRVEGIDSCRFAELMMSSGLVCNDSVSWVTFHSAYDFGYLVKVLTRRSLPS 189
           RR GIDF+RNR EG++S RFAELMMSSGL+CN+SVSWVTFHSAYDFGYLVK+LTRR LP 
Sbjct: 129 RRHGIDFERNRREGVESERFAELMMSSGLICNESVSWVTFHSAYDFGYLVKILTRRQLPV 188

Query: 190 RLEEFLGVLRVFFGDQVYDVKYMMRFCKS-LYGGLDRIAKILNVSRAVGKCHQAGSDSLL 249
            L EFLG+LR FFGD+VYDVK++MRFC+  LYGGLDR+A+ L V+RAVGKCHQAGSDSLL
Sbjct: 189 ALREFLGLLRAFFGDRVYDVKHIMRFCEQRLYGGLDRVARSLEVNRAVGKCHQAGSDSLL 248

Query: 250 TWHAFQKMRDTYFVQNGLEKHAGVLYGLEV 278
           TW AFQ+MRD YFV++G EKHAGVLYGLEV
Sbjct: 249 TWQAFQRMRDLYFVEDGAEKHAGVLYGLEV 277

BLAST of MC03g0451 vs. ExPASy Swiss-Prot
Match: Q9LXM2 (Probable CCR4-associated factor 1 homolog 9 OS=Arabidopsis thaliana OX=3702 GN=CAF1-9 PE=2 SV=1)

HSP 1 Score: 399.8 bits (1026), Expect = 2.5e-110
Identity = 196/276 (71.01%), Postives = 228/276 (82.61%), Query Frame = 0

Query: 4   QDLKNNSKPIQIREVWASNLESEFELIRDLIDQFPCVSMDTEFPGVVFTDPAKPYHRQNR 63
           +DLK +   +  REVWA NLESEFELI ++ID +P +SMDTEFPGV+F    K   R   
Sbjct: 8   RDLKPDGVTVVTREVWAENLESEFELISEIIDDYPFISMDTEFPGVIF----KSDLRFTN 67

Query: 64  PSDHYRLLKSNVDALNLIQIGITLSDSDGNLPDLGTGGSR-FIWEFNFMDFDVARDVHAP 123
           P D Y LLK+NVDAL+LIQ+G+TLSD +GNLPDLG    R FIWEFNF DFDVARD HAP
Sbjct: 68  PDDLYTLLKANVDALSLIQVGLTLSDVNGNLPDLGDDLHRGFIWEFNFRDFDVARDAHAP 127

Query: 124 DSIELLRRQGIDFDRNRVEGIDSCRFAELMMSSGLVCNDSVSWVTFHSAYDFGYLVKVLT 183
           DSIELLRRQGIDF+RN  +G++S RFAELMMSSGLVCN+ VSWVTFHSAYDFGYL+K+LT
Sbjct: 128 DSIELLRRQGIDFERNCRDGVESERFAELMMSSGLVCNEEVSWVTFHSAYDFGYLMKILT 187

Query: 184 RRSLPSRLEEFLGVLRVFFGDQVYDVKYMMRFC-KSLYGGLDRIAKILNVSRAVGKCHQA 243
           RR LP  L EF  V+RV FG++VYDVK+MM+FC + L+GGLDR+A+ L V+RAVGKCHQA
Sbjct: 188 RRELPGALGEFKRVMRVLFGERVYDVKHMMKFCERRLFGGLDRVARTLEVNRAVGKCHQA 247

Query: 244 GSDSLLTWHAFQKMRDTYFVQNGLEKHAGVLYGLEV 278
           GSDSLLTWHAFQ+MRD YFVQ+G EKHAGVLYGLEV
Sbjct: 248 GSDSLLTWHAFQRMRDLYFVQDGPEKHAGVLYGLEV 279

BLAST of MC03g0451 vs. ExPASy Swiss-Prot
Match: Q9SKZ2 (Probable CCR4-associated factor 1 homolog 7 OS=Arabidopsis thaliana OX=3702 GN=CAF1-7 PE=2 SV=2)

HSP 1 Score: 295.4 bits (755), Expect = 6.6e-79
Identity = 148/272 (54.41%), Postives = 201/272 (73.90%), Query Frame = 0

Query: 6   LKNNSKPIQIREVWASNLESEFELIRDLIDQFPCVSMDTEFPGVVFTDPAKPYHRQNRPS 65
           LK++S  IQIREVW  NLESE  LIR+++D FP V+MDTEFPG+V   P   +  +    
Sbjct: 5   LKDDS--IQIREVWNDNLESEMALIREVVDDFPFVAMDTEFPGIV-CRPVGTF--KTNTE 64

Query: 66  DHYRLLKSNVDALNLIQIGITLSDSDGNLPDLGTGGSRFIWEFNFMDFDVARDVHAPDSI 125
            HY  LK+NV+ L +IQ+G+T SD  GNLP  GT     IW+FNF +FD+  D++A DSI
Sbjct: 65  YHYETLKTNVNILKMIQLGLTFSDEKGNLPTCGTDNKYCIWQFNFREFDLESDIYATDSI 124

Query: 126 ELLRRQGIDFDRNRVEGIDSCRFAELMMSSGLVCNDSVSWVTFHSAYDFGYLVKVLTRRS 185
           ELLR+ GIDF +N   GIDS RFAEL+MSSG+V N++V WVTFHS YDFGYL+K+LT ++
Sbjct: 125 ELLRQSGIDFVKNNEFGIDSKRFAELLMSSGIVLNENVHWVTFHSGYDFGYLLKLLTCQN 184

Query: 186 LPSRLEEFLGVLRVFFGDQVYDVKYMMRFCKSLYGGLDRIAKILNVSRAVGKCHQAGSDS 245
           LP     F  ++ V+F  +VYD+K++M+FC SL+GGL+++A++L+V R VG CHQAGSDS
Sbjct: 185 LPETQTGFFEMISVYF-PRVYDIKHLMKFCNSLHGGLNKLAELLDVER-VGICHQAGSDS 244

Query: 246 LLTWHAFQKMRDTYFVQNGLEKHAGVLYGLEV 278
           LLT   F+K+++ +F+   +EK++GVLYGL V
Sbjct: 245 LLTSCTFRKLQENFFI-GSMEKYSGVLYGLGV 268

BLAST of MC03g0451 vs. ExPASy Swiss-Prot
Match: Q9SAI2 (Probable CCR4-associated factor 1 homolog 6 OS=Arabidopsis thaliana OX=3702 GN=CAF1-6 PE=1 SV=1)

HSP 1 Score: 287.3 bits (734), Expect = 1.8e-76
Identity = 146/273 (53.48%), Postives = 200/273 (73.26%), Query Frame = 0

Query: 6   LKNNSKPIQIREVWASNLESEFELIRDLIDQFPCVSMDTEFPGVVFTDPAKPYHRQNRPS 65
           LK++S  IQIREVW  NL+ E +LIRD++D FP V+MDTEFPG+V     +P       +
Sbjct: 5   LKDDS--IQIREVWNDNLQEEMDLIRDVVDDFPYVAMDTEFPGIV----VRPVGTFKSNA 64

Query: 66  D-HYRLLKSNVDALNLIQIGITLSDSDGNLPDLGTGGSRFIWEFNFMDFDVARDVHAPDS 125
           D HY  LK+NV+ L +IQ+G+T S+  GNLP  GT     IW+FNF +FD+  D+ A DS
Sbjct: 65  DYHYETLKTNVNILKMIQLGLTFSNEQGNLPTCGT-DKYCIWQFNFREFDLDSDIFALDS 124

Query: 126 IELLRRQGIDFDRNRVEGIDSCRFAELMMSSGLVCNDSVSWVTFHSAYDFGYLVKVLTRR 185
           IELL++ GID  +N ++GIDS RFAEL+MSSG+V N++V WVTFHS YDFGYL+K+LT +
Sbjct: 125 IELLKQSGIDLAKNTLDGIDSKRFAELLMSSGIVLNENVHWVTFHSGYDFGYLLKLLTCQ 184

Query: 186 SLPSRLEEFLGVLRVFFGDQVYDVKYMMRFCKSLYGGLDRIAKILNVSRAVGKCHQAGSD 245
           +LP    +F  ++ V+F   VYD+K++M+FC SL+GGL+++A++L V R VG CHQAGSD
Sbjct: 185 NLPDSQTDFFKLINVYF-PTVYDIKHLMKFCNSLHGGLNKLAELLEVER-VGICHQAGSD 244

Query: 246 SLLTWHAFQKMRDTYFVQNGLEKHAGVLYGLEV 278
           SLLT   F+K+++ +FV   L K++GVLYGL V
Sbjct: 245 SLLTSCTFRKLKENFFV-GPLHKYSGVLYGLGV 267

BLAST of MC03g0451 vs. ExPASy Swiss-Prot
Match: Q9LEU4 (Probable CCR4-associated factor 1 homolog 10 OS=Arabidopsis thaliana OX=3702 GN=CAF1-10 PE=2 SV=1)

HSP 1 Score: 286.6 bits (732), Expect = 3.1e-76
Identity = 148/277 (53.43%), Postives = 197/277 (71.12%), Query Frame = 0

Query: 2   VVQDLKNNSKPIQIREVWASNLESEFELIRDLIDQFPCVSMDTEFPGVVFTDPAKPYHRQ 61
           + + LK +S  I IREVW  NL  EF LIR+++D+F  ++MDTEFPGVV   P   +   
Sbjct: 1   MAETLKEDS--IMIREVWDYNLVEEFALIREIVDKFSYIAMDTEFPGVVL-KPVATFKYN 60

Query: 62  NRPSDHYRLLKSNVDALNLIQIGITLSDSDGNLPDLGTGGSRF-IWEFNFMDFDVARDVH 121
           N    +YR LK NVD L LIQ+G+T SD +GNLP  GT   +F IW+FNF +F++  D++
Sbjct: 61  N--DLNYRTLKENVDLLKLIQVGLTFSDENGNLPTCGT--DKFCIWQFNFREFNIGEDIY 120

Query: 122 APDSIELLRRQGIDFDRNRVEGIDSCRFAELMMSSGLVCNDSVSWVTFHSAYDFGYLVKV 181
           A +SIELLR+ GIDF +N  +GID  RF ELMMSSG+V ND++SWVTFH  YDFGYLVK+
Sbjct: 121 ASESIELLRQCGIDFKKNIEKGIDVVRFGELMMSSGIVLNDAISWVTFHGGYDFGYLVKL 180

Query: 182 LTRRSLPSRLEEFLGVLRVFFGDQVYDVKYMMRFCKSLYGGLDRIAKILNVSRAVGKCHQ 241
           LT + LP +  +F  +L V+F   VYD+K++M FC  L+GGL+R+A+++ V R VG CHQ
Sbjct: 181 LTCKELPLKQADFFKLLYVYF-PTVYDIKHLMTFCNGLFGGLNRLAELMGVER-VGICHQ 240

Query: 242 AGSDSLLTWHAFQKMRDTYFVQNGLEKHAGVLYGLEV 278
           AGSDSLLT  +F+K+++ YF     EK+ GVLYGL V
Sbjct: 241 AGSDSLLTLGSFRKLKERYF-PGSTEKYTGVLYGLGV 267

BLAST of MC03g0451 vs. NCBI nr
Match: XP_022142408.1 (probable CCR4-associated factor 1 homolog 11 [Momordica charantia])

HSP 1 Score: 573 bits (1477), Expect = 1.84e-206
Identity = 278/278 (100.00%), Postives = 278/278 (100.00%), Query Frame = 0

Query: 1   MVVQDLKNNSKPIQIREVWASNLESEFELIRDLIDQFPCVSMDTEFPGVVFTDPAKPYHR 60
           MVVQDLKNNSKPIQIREVWASNLESEFELIRDLIDQFPCVSMDTEFPGVVFTDPAKPYHR
Sbjct: 1   MVVQDLKNNSKPIQIREVWASNLESEFELIRDLIDQFPCVSMDTEFPGVVFTDPAKPYHR 60

Query: 61  QNRPSDHYRLLKSNVDALNLIQIGITLSDSDGNLPDLGTGGSRFIWEFNFMDFDVARDVH 120
           QNRPSDHYRLLKSNVDALNLIQIGITLSDSDGNLPDLGTGGSRFIWEFNFMDFDVARDVH
Sbjct: 61  QNRPSDHYRLLKSNVDALNLIQIGITLSDSDGNLPDLGTGGSRFIWEFNFMDFDVARDVH 120

Query: 121 APDSIELLRRQGIDFDRNRVEGIDSCRFAELMMSSGLVCNDSVSWVTFHSAYDFGYLVKV 180
           APDSIELLRRQGIDFDRNRVEGIDSCRFAELMMSSGLVCNDSVSWVTFHSAYDFGYLVKV
Sbjct: 121 APDSIELLRRQGIDFDRNRVEGIDSCRFAELMMSSGLVCNDSVSWVTFHSAYDFGYLVKV 180

Query: 181 LTRRSLPSRLEEFLGVLRVFFGDQVYDVKYMMRFCKSLYGGLDRIAKILNVSRAVGKCHQ 240
           LTRRSLPSRLEEFLGVLRVFFGDQVYDVKYMMRFCKSLYGGLDRIAKILNVSRAVGKCHQ
Sbjct: 181 LTRRSLPSRLEEFLGVLRVFFGDQVYDVKYMMRFCKSLYGGLDRIAKILNVSRAVGKCHQ 240

Query: 241 AGSDSLLTWHAFQKMRDTYFVQNGLEKHAGVLYGLEVL 278
           AGSDSLLTWHAFQKMRDTYFVQNGLEKHAGVLYGLEVL
Sbjct: 241 AGSDSLLTWHAFQKMRDTYFVQNGLEKHAGVLYGLEVL 278

BLAST of MC03g0451 vs. NCBI nr
Match: XP_038877224.1 (probable CCR4-associated factor 1 homolog 11 [Benincasa hispida])

HSP 1 Score: 506 bits (1302), Expect = 8.75e-180
Identity = 239/277 (86.28%), Postives = 264/277 (95.31%), Query Frame = 0

Query: 1   MVVQDLKNNSKPIQIREVWASNLESEFELIRDLIDQFPCVSMDTEFPGVVFTDPAKPYHR 60
           MV+ DL  N+K I+IREVWASNLESEF+LIRDLIDQFPCVSMDTEFPG+VF DPA+P++ 
Sbjct: 1   MVIHDLNINNKSIKIREVWASNLESEFQLIRDLIDQFPCVSMDTEFPGIVFADPARPFNF 60

Query: 61  QNRPSDHYRLLKSNVDALNLIQIGITLSDSDGNLPDLGTGGSRFIWEFNFMDFDVARDVH 120
           Q++PSDHYRLLKSNVDAL+LIQIGITLSDSDGNLPDLGT  +RFIWEFNFMDFDVARD+H
Sbjct: 61  QSQPSDHYRLLKSNVDALHLIQIGITLSDSDGNLPDLGTDDTRFIWEFNFMDFDVARDLH 120

Query: 121 APDSIELLRRQGIDFDRNRVEGIDSCRFAELMMSSGLVCNDSVSWVTFHSAYDFGYLVKV 180
           APDSIELLRRQGIDF+RNRVEGIDS RFAELMMSSGLVCN+SVSWVTFHSAYDFGYLVK+
Sbjct: 121 APDSIELLRRQGIDFNRNRVEGIDSSRFAELMMSSGLVCNNSVSWVTFHSAYDFGYLVKI 180

Query: 181 LTRRSLPSRLEEFLGVLRVFFGDQVYDVKYMMRFCKSLYGGLDRIAKILNVSRAVGKCHQ 240
           LT+R LP R++EFLGVLRVFFGD VYD+KY+MRFC+SLYGGLDRIAKILNV+RAVGKCHQ
Sbjct: 181 LTQRRLPDRVDEFLGVLRVFFGDNVYDMKYLMRFCESLYGGLDRIAKILNVNRAVGKCHQ 240

Query: 241 AGSDSLLTWHAFQKMRDTYFVQNGLEKHAGVLYGLEV 277
           AGSDSLLTWHAFQKMRDTYFVQN ++KHAGVLYGLEV
Sbjct: 241 AGSDSLLTWHAFQKMRDTYFVQNEMQKHAGVLYGLEV 277

BLAST of MC03g0451 vs. NCBI nr
Match: XP_022945861.1 (probable CCR4-associated factor 1 homolog 11 [Cucurbita moschata] >KAG6573637.1 putative CCR4-associated factor 1-like 11, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 492 bits (1267), Expect = 2.03e-174
Identity = 238/278 (85.61%), Postives = 257/278 (92.45%), Query Frame = 0

Query: 1   MVVQDLKNNS-KPIQIREVWASNLESEFELIRDLIDQFPCVSMDTEFPGVVFTDPAKPYH 60
           MV  DL NN  K I+IREVWASNLESEF+LIRDLID FPCVSMDTEFPG+VFT P  P++
Sbjct: 1   MVSHDLNNNHYKSIKIREVWASNLESEFQLIRDLIDHFPCVSMDTEFPGIVFTAPTAPFN 60

Query: 61  RQNRPS-DHYRLLKSNVDALNLIQIGITLSDSDGNLPDLGTGGSRFIWEFNFMDFDVARD 120
             NR S DHY+LLKSNVDALNLIQIGITLSDSDGNLPDLGT  SRFIWEFNFMDFD+ARD
Sbjct: 61  ALNRSSSDHYKLLKSNVDALNLIQIGITLSDSDGNLPDLGTDDSRFIWEFNFMDFDLARD 120

Query: 121 VHAPDSIELLRRQGIDFDRNRVEGIDSCRFAELMMSSGLVCNDSVSWVTFHSAYDFGYLV 180
           ++APDSI+LLRRQGIDFDRNRVEGIDSCRFAELMMSSGLVCNDSVSWVTFHSAYDF YL+
Sbjct: 121 IYAPDSIDLLRRQGIDFDRNRVEGIDSCRFAELMMSSGLVCNDSVSWVTFHSAYDFAYLI 180

Query: 181 KVLTRRSLPSRLEEFLGVLRVFFGDQVYDVKYMMRFCKSLYGGLDRIAKILNVSRAVGKC 240
           K+LT++SLP+RL+EFLGVLRV FGD VYDVKYMMRFCKSLYGGLDRIAK LNV+RAVGKC
Sbjct: 181 KILTQKSLPNRLDEFLGVLRVLFGDNVYDVKYMMRFCKSLYGGLDRIAKSLNVNRAVGKC 240

Query: 241 HQAGSDSLLTWHAFQKMRDTYFVQNGLEKHAGVLYGLE 276
           HQAGSDSLLTWHAFQKMRDT+FVQN +EKHAGVLYGLE
Sbjct: 241 HQAGSDSLLTWHAFQKMRDTFFVQNEVEKHAGVLYGLE 278

BLAST of MC03g0451 vs. NCBI nr
Match: XP_022967098.1 (probable CCR4-associated factor 1 homolog 11 [Cucurbita maxima])

HSP 1 Score: 489 bits (1259), Expect = 3.36e-173
Identity = 236/278 (84.89%), Postives = 256/278 (92.09%), Query Frame = 0

Query: 1   MVVQDLKNNS-KPIQIREVWASNLESEFELIRDLIDQFPCVSMDTEFPGVVFTDPAKPYH 60
           MV  DL NN  K I+IREVWASNLESEF+LIRDLID FPCVSMDTEFPG+VFT P  P++
Sbjct: 1   MVSHDLNNNHYKSIKIREVWASNLESEFQLIRDLIDHFPCVSMDTEFPGIVFTAPTAPFN 60

Query: 61  RQNRPS-DHYRLLKSNVDALNLIQIGITLSDSDGNLPDLGTGGSRFIWEFNFMDFDVARD 120
             NR S DHY+LLKSNVDALNLIQIGITLSDSDGNLPDLGT  SRFIWEFNFMDFD+ARD
Sbjct: 61  SLNRSSSDHYKLLKSNVDALNLIQIGITLSDSDGNLPDLGTDDSRFIWEFNFMDFDLARD 120

Query: 121 VHAPDSIELLRRQGIDFDRNRVEGIDSCRFAELMMSSGLVCNDSVSWVTFHSAYDFGYLV 180
           ++APDSI+LLRRQGIDFDRNRVEGIDSCRFAELMMSSGLVCNDSVSWVTFHSAYDF YL+
Sbjct: 121 IYAPDSIDLLRRQGIDFDRNRVEGIDSCRFAELMMSSGLVCNDSVSWVTFHSAYDFAYLI 180

Query: 181 KVLTRRSLPSRLEEFLGVLRVFFGDQVYDVKYMMRFCKSLYGGLDRIAKILNVSRAVGKC 240
           K+LT++ LP+R++EFLGVLRV FGD VYDVKYMMRFCKSLYGGLDRIAK LNV+RAVGKC
Sbjct: 181 KILTQKRLPNRVDEFLGVLRVLFGDNVYDVKYMMRFCKSLYGGLDRIAKSLNVNRAVGKC 240

Query: 241 HQAGSDSLLTWHAFQKMRDTYFVQNGLEKHAGVLYGLE 276
           HQAGSDSLLTWHAFQKMRDT+FVQN +EKHAGVLYGLE
Sbjct: 241 HQAGSDSLLTWHAFQKMRDTFFVQNEVEKHAGVLYGLE 278

BLAST of MC03g0451 vs. NCBI nr
Match: KAG7012717.1 (putative CCR4-associated factor 1-like 11, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 488 bits (1256), Expect = 9.63e-173
Identity = 236/278 (84.89%), Postives = 256/278 (92.09%), Query Frame = 0

Query: 1   MVVQDLKNNS-KPIQIREVWASNLESEFELIRDLIDQFPCVSMDTEFPGVVFTDPAKPYH 60
           MV  DL +N  K I+IREVWASNLESEF+LIRD ID FPCVSMDTEFPG+VFT P  P++
Sbjct: 1   MVSHDLNDNHYKSIKIREVWASNLESEFQLIRDSIDHFPCVSMDTEFPGIVFTAPTAPFN 60

Query: 61  RQNRPS-DHYRLLKSNVDALNLIQIGITLSDSDGNLPDLGTGGSRFIWEFNFMDFDVARD 120
             NR S DHY+LLKSNVDALNLIQIGITLSDSDGNLPDLGT  SRFIWEFNFMDFD+ARD
Sbjct: 61  ALNRSSSDHYKLLKSNVDALNLIQIGITLSDSDGNLPDLGTDDSRFIWEFNFMDFDLARD 120

Query: 121 VHAPDSIELLRRQGIDFDRNRVEGIDSCRFAELMMSSGLVCNDSVSWVTFHSAYDFGYLV 180
           ++APDSI+LLRRQGIDFDRNRVEGIDSCRFAELMMSSGLVCNDSVSWVTFHSAYDF YL+
Sbjct: 121 IYAPDSIDLLRRQGIDFDRNRVEGIDSCRFAELMMSSGLVCNDSVSWVTFHSAYDFAYLI 180

Query: 181 KVLTRRSLPSRLEEFLGVLRVFFGDQVYDVKYMMRFCKSLYGGLDRIAKILNVSRAVGKC 240
           K+LT++SLP+RL+EFLGVLRV FGD VYDVKYMMRFCKSLYGGLDRIAK LNV+RAVGKC
Sbjct: 181 KILTQKSLPNRLDEFLGVLRVLFGDNVYDVKYMMRFCKSLYGGLDRIAKSLNVNRAVGKC 240

Query: 241 HQAGSDSLLTWHAFQKMRDTYFVQNGLEKHAGVLYGLE 276
           HQAGSDSLLTWHAFQKMRDT+FVQN +EKHAGVLYGLE
Sbjct: 241 HQAGSDSLLTWHAFQKMRDTFFVQNEVEKHAGVLYGLE 278

BLAST of MC03g0451 vs. ExPASy TrEMBL
Match: A0A6J1CN71 (Poly(A)-specific ribonuclease OS=Momordica charantia OX=3673 GN=LOC111012547 PE=3 SV=1)

HSP 1 Score: 573 bits (1477), Expect = 8.90e-207
Identity = 278/278 (100.00%), Postives = 278/278 (100.00%), Query Frame = 0

Query: 1   MVVQDLKNNSKPIQIREVWASNLESEFELIRDLIDQFPCVSMDTEFPGVVFTDPAKPYHR 60
           MVVQDLKNNSKPIQIREVWASNLESEFELIRDLIDQFPCVSMDTEFPGVVFTDPAKPYHR
Sbjct: 1   MVVQDLKNNSKPIQIREVWASNLESEFELIRDLIDQFPCVSMDTEFPGVVFTDPAKPYHR 60

Query: 61  QNRPSDHYRLLKSNVDALNLIQIGITLSDSDGNLPDLGTGGSRFIWEFNFMDFDVARDVH 120
           QNRPSDHYRLLKSNVDALNLIQIGITLSDSDGNLPDLGTGGSRFIWEFNFMDFDVARDVH
Sbjct: 61  QNRPSDHYRLLKSNVDALNLIQIGITLSDSDGNLPDLGTGGSRFIWEFNFMDFDVARDVH 120

Query: 121 APDSIELLRRQGIDFDRNRVEGIDSCRFAELMMSSGLVCNDSVSWVTFHSAYDFGYLVKV 180
           APDSIELLRRQGIDFDRNRVEGIDSCRFAELMMSSGLVCNDSVSWVTFHSAYDFGYLVKV
Sbjct: 121 APDSIELLRRQGIDFDRNRVEGIDSCRFAELMMSSGLVCNDSVSWVTFHSAYDFGYLVKV 180

Query: 181 LTRRSLPSRLEEFLGVLRVFFGDQVYDVKYMMRFCKSLYGGLDRIAKILNVSRAVGKCHQ 240
           LTRRSLPSRLEEFLGVLRVFFGDQVYDVKYMMRFCKSLYGGLDRIAKILNVSRAVGKCHQ
Sbjct: 181 LTRRSLPSRLEEFLGVLRVFFGDQVYDVKYMMRFCKSLYGGLDRIAKILNVSRAVGKCHQ 240

Query: 241 AGSDSLLTWHAFQKMRDTYFVQNGLEKHAGVLYGLEVL 278
           AGSDSLLTWHAFQKMRDTYFVQNGLEKHAGVLYGLEVL
Sbjct: 241 AGSDSLLTWHAFQKMRDTYFVQNGLEKHAGVLYGLEVL 278

BLAST of MC03g0451 vs. ExPASy TrEMBL
Match: A0A6J1G223 (Poly(A)-specific ribonuclease OS=Cucurbita moschata OX=3662 GN=LOC111449978 PE=3 SV=1)

HSP 1 Score: 492 bits (1267), Expect = 9.83e-175
Identity = 238/278 (85.61%), Postives = 257/278 (92.45%), Query Frame = 0

Query: 1   MVVQDLKNNS-KPIQIREVWASNLESEFELIRDLIDQFPCVSMDTEFPGVVFTDPAKPYH 60
           MV  DL NN  K I+IREVWASNLESEF+LIRDLID FPCVSMDTEFPG+VFT P  P++
Sbjct: 1   MVSHDLNNNHYKSIKIREVWASNLESEFQLIRDLIDHFPCVSMDTEFPGIVFTAPTAPFN 60

Query: 61  RQNRPS-DHYRLLKSNVDALNLIQIGITLSDSDGNLPDLGTGGSRFIWEFNFMDFDVARD 120
             NR S DHY+LLKSNVDALNLIQIGITLSDSDGNLPDLGT  SRFIWEFNFMDFD+ARD
Sbjct: 61  ALNRSSSDHYKLLKSNVDALNLIQIGITLSDSDGNLPDLGTDDSRFIWEFNFMDFDLARD 120

Query: 121 VHAPDSIELLRRQGIDFDRNRVEGIDSCRFAELMMSSGLVCNDSVSWVTFHSAYDFGYLV 180
           ++APDSI+LLRRQGIDFDRNRVEGIDSCRFAELMMSSGLVCNDSVSWVTFHSAYDF YL+
Sbjct: 121 IYAPDSIDLLRRQGIDFDRNRVEGIDSCRFAELMMSSGLVCNDSVSWVTFHSAYDFAYLI 180

Query: 181 KVLTRRSLPSRLEEFLGVLRVFFGDQVYDVKYMMRFCKSLYGGLDRIAKILNVSRAVGKC 240
           K+LT++SLP+RL+EFLGVLRV FGD VYDVKYMMRFCKSLYGGLDRIAK LNV+RAVGKC
Sbjct: 181 KILTQKSLPNRLDEFLGVLRVLFGDNVYDVKYMMRFCKSLYGGLDRIAKSLNVNRAVGKC 240

Query: 241 HQAGSDSLLTWHAFQKMRDTYFVQNGLEKHAGVLYGLE 276
           HQAGSDSLLTWHAFQKMRDT+FVQN +EKHAGVLYGLE
Sbjct: 241 HQAGSDSLLTWHAFQKMRDTFFVQNEVEKHAGVLYGLE 278

BLAST of MC03g0451 vs. ExPASy TrEMBL
Match: A0A6J1HU54 (Poly(A)-specific ribonuclease OS=Cucurbita maxima OX=3661 GN=LOC111466603 PE=3 SV=1)

HSP 1 Score: 489 bits (1259), Expect = 1.63e-173
Identity = 236/278 (84.89%), Postives = 256/278 (92.09%), Query Frame = 0

Query: 1   MVVQDLKNNS-KPIQIREVWASNLESEFELIRDLIDQFPCVSMDTEFPGVVFTDPAKPYH 60
           MV  DL NN  K I+IREVWASNLESEF+LIRDLID FPCVSMDTEFPG+VFT P  P++
Sbjct: 1   MVSHDLNNNHYKSIKIREVWASNLESEFQLIRDLIDHFPCVSMDTEFPGIVFTAPTAPFN 60

Query: 61  RQNRPS-DHYRLLKSNVDALNLIQIGITLSDSDGNLPDLGTGGSRFIWEFNFMDFDVARD 120
             NR S DHY+LLKSNVDALNLIQIGITLSDSDGNLPDLGT  SRFIWEFNFMDFD+ARD
Sbjct: 61  SLNRSSSDHYKLLKSNVDALNLIQIGITLSDSDGNLPDLGTDDSRFIWEFNFMDFDLARD 120

Query: 121 VHAPDSIELLRRQGIDFDRNRVEGIDSCRFAELMMSSGLVCNDSVSWVTFHSAYDFGYLV 180
           ++APDSI+LLRRQGIDFDRNRVEGIDSCRFAELMMSSGLVCNDSVSWVTFHSAYDF YL+
Sbjct: 121 IYAPDSIDLLRRQGIDFDRNRVEGIDSCRFAELMMSSGLVCNDSVSWVTFHSAYDFAYLI 180

Query: 181 KVLTRRSLPSRLEEFLGVLRVFFGDQVYDVKYMMRFCKSLYGGLDRIAKILNVSRAVGKC 240
           K+LT++ LP+R++EFLGVLRV FGD VYDVKYMMRFCKSLYGGLDRIAK LNV+RAVGKC
Sbjct: 181 KILTQKRLPNRVDEFLGVLRVLFGDNVYDVKYMMRFCKSLYGGLDRIAKSLNVNRAVGKC 240

Query: 241 HQAGSDSLLTWHAFQKMRDTYFVQNGLEKHAGVLYGLE 276
           HQAGSDSLLTWHAFQKMRDT+FVQN +EKHAGVLYGLE
Sbjct: 241 HQAGSDSLLTWHAFQKMRDTFFVQNEVEKHAGVLYGLE 278

BLAST of MC03g0451 vs. ExPASy TrEMBL
Match: A0A5J5C461 (Poly(A)-specific ribonuclease OS=Nyssa sinensis OX=561372 GN=F0562_001613 PE=3 SV=1)

HSP 1 Score: 472 bits (1214), Expect = 2.89e-166
Identity = 224/276 (81.16%), Postives = 250/276 (90.58%), Query Frame = 0

Query: 5   DLKNNSKPIQIREVWASNLESEFELIRDLIDQFPCVSMDTEFPGVVF---TDPAKPYHRQ 64
           D +   KPI IREVWA NLESEFELI DLID++P +SMDTEFPGVVF    DPAKPYHRQ
Sbjct: 30  DPEPKPKPIVIREVWADNLESEFELISDLIDRYPYISMDTEFPGVVFRPAVDPAKPYHRQ 89

Query: 65  NRPSDHYRLLKSNVDALNLIQIGITLSDSDGNLPDLGTGGSRFIWEFNFMDFDVARDVHA 124
            RPSDHY++LKSNVDALNLIQ+G+TLSD+ GNLPD+GTG  RFIWEFNF DFDV RDVHA
Sbjct: 90  RRPSDHYKILKSNVDALNLIQLGLTLSDATGNLPDIGTG-HRFIWEFNFSDFDVMRDVHA 149

Query: 125 PDSIELLRRQGIDFDRNRVEGIDSCRFAELMMSSGLVCNDSVSWVTFHSAYDFGYLVKVL 184
           PDS+ELL+RQGIDFDRNRVEG+DS RFAELMMSSGLVCNDSVSWVTFHSAYDFGYLVK+L
Sbjct: 150 PDSVELLKRQGIDFDRNRVEGVDSARFAELMMSSGLVCNDSVSWVTFHSAYDFGYLVKIL 209

Query: 185 TRRSLPSRLEEFLGVLRVFFGDQVYDVKYMMRFCKSLYGGLDRIAKILNVSRAVGKCHQA 244
           TRR LP+ L++FLGVLRVFFG++VYDVK++M+FC+SLYGGLDR+A+ L V RAVGKCHQA
Sbjct: 210 TRRELPTGLKDFLGVLRVFFGNRVYDVKHLMKFCESLYGGLDRVARTLEVDRAVGKCHQA 269

Query: 245 GSDSLLTWHAFQKMRDTYFVQNGLEKHAGVLYGLEV 277
           GSDSLLTWHAFQKMRD YFV+NG EKHAGVLYGLEV
Sbjct: 270 GSDSLLTWHAFQKMRDVYFVKNGPEKHAGVLYGLEV 304

BLAST of MC03g0451 vs. ExPASy TrEMBL
Match: A0A0A0LYC8 (Poly(A)-specific ribonuclease OS=Cucumis sativus OX=3659 GN=Csa_1G168920 PE=3 SV=1)

HSP 1 Score: 469 bits (1206), Expect = 1.73e-165
Identity = 225/277 (81.23%), Postives = 245/277 (88.45%), Query Frame = 0

Query: 1   MVVQDLKNNSKPIQIREVWASNLESEFELIRDLIDQFPCVSMDTEFPGVVFTDPAKPYHR 60
           M++ DL  N K IQIREVWASNLESEF LIRDLIDQFPCVSMDTEFPGV+F     P   
Sbjct: 1   MLIHDLNTNHKSIQIREVWASNLESEFRLIRDLIDQFPCVSMDTEFPGVIFPQQDIP-KS 60

Query: 61  QNRPSDHYRLLKSNVDALNLIQIGITLSDSDGNLPDLGTGGSRFIWEFNFMDFDVARDVH 120
            N PS HY+ LKSNVD L+LIQIGITLSDSDGNLPDLGTG +RFIWEFNF DFDVARD+H
Sbjct: 61  PNHPSHHYQFLKSNVDLLHLIQIGITLSDSDGNLPDLGTGDTRFIWEFNFKDFDVARDLH 120

Query: 121 APDSIELLRRQGIDFDRNRVEGIDSCRFAELMMSSGLVCNDSVSWVTFHSAYDFGYLVKV 180
           AP+SIELLRRQGIDFDRNR EGIDS RFAELMMSSGLVCNDSVSWVTFHSAYDFGYLVK+
Sbjct: 121 APNSIELLRRQGIDFDRNREEGIDSSRFAELMMSSGLVCNDSVSWVTFHSAYDFGYLVKI 180

Query: 181 LTRRSLPSRLEEFLGVLRVFFGDQVYDVKYMMRFCKSLYGGLDRIAKILNVSRAVGKCHQ 240
           LT + LP  LE+FLGV+R+FFGD VYD+KY+MRFC+SLYGGLDRIAK +NV+RAVGKCHQ
Sbjct: 181 LTHQKLPKDLEQFLGVVRLFFGDNVYDMKYLMRFCQSLYGGLDRIAKTMNVNRAVGKCHQ 240

Query: 241 AGSDSLLTWHAFQKMRDTYFVQNGLEKHAGVLYGLEV 277
           AGSDSLLTWHAFQKMRDT+FVQ  + KHAGVLYGLEV
Sbjct: 241 AGSDSLLTWHAFQKMRDTFFVQTEMHKHAGVLYGLEV 276

BLAST of MC03g0451 vs. TAIR 10
Match: AT5G22250.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 402.5 bits (1033), Expect = 2.7e-112
Identity = 195/270 (72.22%), Postives = 227/270 (84.07%), Query Frame = 0

Query: 10  SKPIQIREVWASNLESEFELIRDLIDQFPCVSMDTEFPGVVFTDPAKPYHRQNRPSDHYR 69
           S  I IR+VWA NLESEF+LIR +++ +P +SMDTEFPGV++        R N P+  Y 
Sbjct: 9   SDVIVIRDVWAYNLESEFDLIRGIVEDYPFISMDTEFPGVIYKADLDVLRRGN-PNYLYN 68

Query: 70  LLKSNVDALNLIQIGITLSDSDGNLPDL-GTGGSRFIWEFNFMDFDVARDVHAPDSIELL 129
           LLKSNVDAL+LIQ+G+TLSD+DGNLPDL G    R+IWEFNF DFDV RD HAPDSIELL
Sbjct: 69  LLKSNVDALSLIQVGLTLSDADGNLPDLGGQKNRRYIWEFNFRDFDVERDPHAPDSIELL 128

Query: 130 RRQGIDFDRNRVEGIDSCRFAELMMSSGLVCNDSVSWVTFHSAYDFGYLVKVLTRRSLPS 189
           RR GIDF+RNR EG++S RFAELMMSSGL+CN+SVSWVTFHSAYDFGYLVK+LTRR LP 
Sbjct: 129 RRHGIDFERNRREGVESERFAELMMSSGLICNESVSWVTFHSAYDFGYLVKILTRRQLPV 188

Query: 190 RLEEFLGVLRVFFGDQVYDVKYMMRFCKS-LYGGLDRIAKILNVSRAVGKCHQAGSDSLL 249
            L EFLG+LR FFGD+VYDVK++MRFC+  LYGGLDR+A+ L V+RAVGKCHQAGSDSLL
Sbjct: 189 ALREFLGLLRAFFGDRVYDVKHIMRFCEQRLYGGLDRVARSLEVNRAVGKCHQAGSDSLL 248

Query: 250 TWHAFQKMRDTYFVQNGLEKHAGVLYGLEV 278
           TW AFQ+MRD YFV++G EKHAGVLYGLEV
Sbjct: 249 TWQAFQRMRDLYFVEDGAEKHAGVLYGLEV 277

BLAST of MC03g0451 vs. TAIR 10
Match: AT3G44260.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 399.8 bits (1026), Expect = 1.8e-111
Identity = 196/276 (71.01%), Postives = 228/276 (82.61%), Query Frame = 0

Query: 4   QDLKNNSKPIQIREVWASNLESEFELIRDLIDQFPCVSMDTEFPGVVFTDPAKPYHRQNR 63
           +DLK +   +  REVWA NLESEFELI ++ID +P +SMDTEFPGV+F    K   R   
Sbjct: 8   RDLKPDGVTVVTREVWAENLESEFELISEIIDDYPFISMDTEFPGVIF----KSDLRFTN 67

Query: 64  PSDHYRLLKSNVDALNLIQIGITLSDSDGNLPDLGTGGSR-FIWEFNFMDFDVARDVHAP 123
           P D Y LLK+NVDAL+LIQ+G+TLSD +GNLPDLG    R FIWEFNF DFDVARD HAP
Sbjct: 68  PDDLYTLLKANVDALSLIQVGLTLSDVNGNLPDLGDDLHRGFIWEFNFRDFDVARDAHAP 127

Query: 124 DSIELLRRQGIDFDRNRVEGIDSCRFAELMMSSGLVCNDSVSWVTFHSAYDFGYLVKVLT 183
           DSIELLRRQGIDF+RN  +G++S RFAELMMSSGLVCN+ VSWVTFHSAYDFGYL+K+LT
Sbjct: 128 DSIELLRRQGIDFERNCRDGVESERFAELMMSSGLVCNEEVSWVTFHSAYDFGYLMKILT 187

Query: 184 RRSLPSRLEEFLGVLRVFFGDQVYDVKYMMRFC-KSLYGGLDRIAKILNVSRAVGKCHQA 243
           RR LP  L EF  V+RV FG++VYDVK+MM+FC + L+GGLDR+A+ L V+RAVGKCHQA
Sbjct: 188 RRELPGALGEFKRVMRVLFGERVYDVKHMMKFCERRLFGGLDRVARTLEVNRAVGKCHQA 247

Query: 244 GSDSLLTWHAFQKMRDTYFVQNGLEKHAGVLYGLEV 278
           GSDSLLTWHAFQ+MRD YFVQ+G EKHAGVLYGLEV
Sbjct: 248 GSDSLLTWHAFQRMRDLYFVQDGPEKHAGVLYGLEV 279

BLAST of MC03g0451 vs. TAIR 10
Match: AT2G32070.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 295.4 bits (755), Expect = 4.7e-80
Identity = 148/272 (54.41%), Postives = 201/272 (73.90%), Query Frame = 0

Query: 6   LKNNSKPIQIREVWASNLESEFELIRDLIDQFPCVSMDTEFPGVVFTDPAKPYHRQNRPS 65
           LK++S  IQIREVW  NLESE  LIR+++D FP V+MDTEFPG+V   P   +  +    
Sbjct: 5   LKDDS--IQIREVWNDNLESEMALIREVVDDFPFVAMDTEFPGIV-CRPVGTF--KTNTE 64

Query: 66  DHYRLLKSNVDALNLIQIGITLSDSDGNLPDLGTGGSRFIWEFNFMDFDVARDVHAPDSI 125
            HY  LK+NV+ L +IQ+G+T SD  GNLP  GT     IW+FNF +FD+  D++A DSI
Sbjct: 65  YHYETLKTNVNILKMIQLGLTFSDEKGNLPTCGTDNKYCIWQFNFREFDLESDIYATDSI 124

Query: 126 ELLRRQGIDFDRNRVEGIDSCRFAELMMSSGLVCNDSVSWVTFHSAYDFGYLVKVLTRRS 185
           ELLR+ GIDF +N   GIDS RFAEL+MSSG+V N++V WVTFHS YDFGYL+K+LT ++
Sbjct: 125 ELLRQSGIDFVKNNEFGIDSKRFAELLMSSGIVLNENVHWVTFHSGYDFGYLLKLLTCQN 184

Query: 186 LPSRLEEFLGVLRVFFGDQVYDVKYMMRFCKSLYGGLDRIAKILNVSRAVGKCHQAGSDS 245
           LP     F  ++ V+F  +VYD+K++M+FC SL+GGL+++A++L+V R VG CHQAGSDS
Sbjct: 185 LPETQTGFFEMISVYF-PRVYDIKHLMKFCNSLHGGLNKLAELLDVER-VGICHQAGSDS 244

Query: 246 LLTWHAFQKMRDTYFVQNGLEKHAGVLYGLEV 278
           LLT   F+K+++ +F+   +EK++GVLYGL V
Sbjct: 245 LLTSCTFRKLQENFFI-GSMEKYSGVLYGLGV 268

BLAST of MC03g0451 vs. TAIR 10
Match: AT1G80780.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 287.3 bits (734), Expect = 1.3e-77
Identity = 146/273 (53.48%), Postives = 200/273 (73.26%), Query Frame = 0

Query: 6   LKNNSKPIQIREVWASNLESEFELIRDLIDQFPCVSMDTEFPGVVFTDPAKPYHRQNRPS 65
           LK++S  IQIREVW  NL+ E +LIRD++D FP V+MDTEFPG+V     +P       +
Sbjct: 5   LKDDS--IQIREVWNDNLQEEMDLIRDVVDDFPYVAMDTEFPGIV----VRPVGTFKSNA 64

Query: 66  D-HYRLLKSNVDALNLIQIGITLSDSDGNLPDLGTGGSRFIWEFNFMDFDVARDVHAPDS 125
           D HY  LK+NV+ L +IQ+G+T S+  GNLP  GT     IW+FNF +FD+  D+ A DS
Sbjct: 65  DYHYETLKTNVNILKMIQLGLTFSNEQGNLPTCGT-DKYCIWQFNFREFDLDSDIFALDS 124

Query: 126 IELLRRQGIDFDRNRVEGIDSCRFAELMMSSGLVCNDSVSWVTFHSAYDFGYLVKVLTRR 185
           IELL++ GID  +N ++GIDS RFAEL+MSSG+V N++V WVTFHS YDFGYL+K+LT +
Sbjct: 125 IELLKQSGIDLAKNTLDGIDSKRFAELLMSSGIVLNENVHWVTFHSGYDFGYLLKLLTCQ 184

Query: 186 SLPSRLEEFLGVLRVFFGDQVYDVKYMMRFCKSLYGGLDRIAKILNVSRAVGKCHQAGSD 245
           +LP    +F  ++ V+F   VYD+K++M+FC SL+GGL+++A++L V R VG CHQAGSD
Sbjct: 185 NLPDSQTDFFKLINVYF-PTVYDIKHLMKFCNSLHGGLNKLAELLEVER-VGICHQAGSD 244

Query: 246 SLLTWHAFQKMRDTYFVQNGLEKHAGVLYGLEV 278
           SLLT   F+K+++ +FV   L K++GVLYGL V
Sbjct: 245 SLLTSCTFRKLKENFFV-GPLHKYSGVLYGLGV 267

BLAST of MC03g0451 vs. TAIR 10
Match: AT1G80780.2 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 287.3 bits (734), Expect = 1.3e-77
Identity = 146/273 (53.48%), Postives = 200/273 (73.26%), Query Frame = 0

Query: 6   LKNNSKPIQIREVWASNLESEFELIRDLIDQFPCVSMDTEFPGVVFTDPAKPYHRQNRPS 65
           LK++S  IQIREVW  NL+ E +LIRD++D FP V+MDTEFPG+V     +P       +
Sbjct: 5   LKDDS--IQIREVWNDNLQEEMDLIRDVVDDFPYVAMDTEFPGIV----VRPVGTFKSNA 64

Query: 66  D-HYRLLKSNVDALNLIQIGITLSDSDGNLPDLGTGGSRFIWEFNFMDFDVARDVHAPDS 125
           D HY  LK+NV+ L +IQ+G+T S+  GNLP  GT     IW+FNF +FD+  D+ A DS
Sbjct: 65  DYHYETLKTNVNILKMIQLGLTFSNEQGNLPTCGT-DKYCIWQFNFREFDLDSDIFALDS 124

Query: 126 IELLRRQGIDFDRNRVEGIDSCRFAELMMSSGLVCNDSVSWVTFHSAYDFGYLVKVLTRR 185
           IELL++ GID  +N ++GIDS RFAEL+MSSG+V N++V WVTFHS YDFGYL+K+LT +
Sbjct: 125 IELLKQSGIDLAKNTLDGIDSKRFAELLMSSGIVLNENVHWVTFHSGYDFGYLLKLLTCQ 184

Query: 186 SLPSRLEEFLGVLRVFFGDQVYDVKYMMRFCKSLYGGLDRIAKILNVSRAVGKCHQAGSD 245
           +LP    +F  ++ V+F   VYD+K++M+FC SL+GGL+++A++L V R VG CHQAGSD
Sbjct: 185 NLPDSQTDFFKLINVYF-PTVYDIKHLMKFCNSLHGGLNKLAELLEVER-VGICHQAGSD 244

Query: 246 SLLTWHAFQKMRDTYFVQNGLEKHAGVLYGLEV 278
           SLLT   F+K+++ +FV   L K++GVLYGL V
Sbjct: 245 SLLTSCTFRKLKENFFV-GPLHKYSGVLYGLGV 267

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FMS63.8e-11172.22Probable CCR4-associated factor 1 homolog 11 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q9LXM22.5e-11071.01Probable CCR4-associated factor 1 homolog 9 OS=Arabidopsis thaliana OX=3702 GN=C... [more]
Q9SKZ26.6e-7954.41Probable CCR4-associated factor 1 homolog 7 OS=Arabidopsis thaliana OX=3702 GN=C... [more]
Q9SAI21.8e-7653.48Probable CCR4-associated factor 1 homolog 6 OS=Arabidopsis thaliana OX=3702 GN=C... [more]
Q9LEU43.1e-7653.43Probable CCR4-associated factor 1 homolog 10 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Match NameE-valueIdentityDescription
XP_022142408.11.84e-206100.00probable CCR4-associated factor 1 homolog 11 [Momordica charantia][more]
XP_038877224.18.75e-18086.28probable CCR4-associated factor 1 homolog 11 [Benincasa hispida][more]
XP_022945861.12.03e-17485.61probable CCR4-associated factor 1 homolog 11 [Cucurbita moschata] >KAG6573637.1 ... [more]
XP_022967098.13.36e-17384.89probable CCR4-associated factor 1 homolog 11 [Cucurbita maxima][more]
KAG7012717.19.63e-17384.89putative CCR4-associated factor 1-like 11, partial [Cucurbita argyrosperma subsp... [more]
Match NameE-valueIdentityDescription
A0A6J1CN718.90e-207100.00Poly(A)-specific ribonuclease OS=Momordica charantia OX=3673 GN=LOC111012547 PE=... [more]
A0A6J1G2239.83e-17585.61Poly(A)-specific ribonuclease OS=Cucurbita moschata OX=3662 GN=LOC111449978 PE=3... [more]
A0A6J1HU541.63e-17384.89Poly(A)-specific ribonuclease OS=Cucurbita maxima OX=3661 GN=LOC111466603 PE=3 S... [more]
A0A5J5C4612.89e-16681.16Poly(A)-specific ribonuclease OS=Nyssa sinensis OX=561372 GN=F0562_001613 PE=3 S... [more]
A0A0A0LYC81.73e-16581.23Poly(A)-specific ribonuclease OS=Cucumis sativus OX=3659 GN=Csa_1G168920 PE=3 SV... [more]
Match NameE-valueIdentityDescription
AT5G22250.12.7e-11272.22Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT3G44260.11.8e-11171.01Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT2G32070.14.7e-8054.41Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT1G80780.11.3e-7753.48Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT1G80780.21.3e-7753.48Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006941Ribonuclease CAF1PFAMPF04857CAF1coord: 18..144
e-value: 2.8E-10
score: 40.0
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 2..277
e-value: 5.0E-96
score: 323.0
NoneNo IPR availablePANTHERPTHR10797:SF59RIBONUCLEASE CAF1, RIBONUCLEASE H-LIKE DOMAIN PROTEIN-RELATEDcoord: 11..277
IPR039637CCR4-NOT transcription complex subunit 7/8/Pop2PANTHERPTHR10797CCR4-NOT TRANSCRIPTION COMPLEX SUBUNITcoord: 11..277
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 15..275

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC03g0451.1MC03g0451.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043928 exonucleolytic catabolism of deadenylated mRNA
biological_process GO:0090503 RNA phosphodiester bond hydrolysis, exonucleolytic
cellular_component GO:0030015 CCR4-NOT core complex
cellular_component GO:0005634 nucleus
cellular_component GO:0000932 P-body
cellular_component GO:0030014 CCR4-NOT complex
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004535 poly(A)-specific ribonuclease activity