MC03g0424 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC03g0424
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Calcium-binding EF-hand family protein
Location	MC03: 11163611 .. 11167029 (-)
RNA-Seq Expression	MC03g0424
Synteny	MC03g0424

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

TATTAATTGGTTTAAAATATCGAAATCTCCATTTTGAATATTGAAGCACGTTTTTTGAGGAATTGATCGGCCGAAGATTTGAAGAAATGGACGCGAAACAAACGAACAGCAGAAAGAAATCAAACCCGTGATCTCATCTTTCATTGAATCGAAGCAACCGCGTCTTCTTTCTACAGAATTCGTCTTTGATTTTTCCTTTTACTTCCCTCTGTTGCATCAATTTTATCTTTTTTCTCTGTCTTTCTCCCATCTTTTCCGTTTAAATTCCCACGCACGAAACCAAAAGTTGCTCCCCAGATTCGAATCCCCTTCTGATTCAATTTCTTGTTCTAACCCATCTTCTAATTCTCTCTTGCTCTTCTTGTTGGCCATCAATGGCGTCCTTCTTTGGAAGATTTACTTCCCATTCCCATTCCAATTCCAATGCTCCATCCGCTCCATCCGCGCCGTCACCACCGCCCGCGCCGGAAACCCACGACCAGGGCCAATCTCACGGGACCGCCGCGCAGTATTACTCCGAACAGCAGCGGCCGCGGCCTTATGGGTCTGATTATGGCGGGGTTTCTTCATATGGGTCCTACGGTTTTCCGCCGGGGACAAGCCCTGAGGTCATTAGGAGCTTTCAAATGGTGGATAGGGATGGAAGTGGATTCATCGATGAGAATGAATTGCAGCAAGCCCTTTCTTCTGGGTACCAGAGGTTTAGTCTTAGAACCGTTCGTTTGCTCATCTTCTTATTCAGAAATCCGGTTGATTCCACCAGAATGGGTGAGTTTCTTCTTCTACTTCTTCATTCCTTCCTTAAATCAAAATCTCGATGTTTCCTTAGATTCATGGTTAAGTTACACGTACAATCCAATCCCTGTTTGGGTTGTGAATATTAGAAACTTAACCTTGATTTAATGTGGCAAATGGGTACAATCACGAATTCTAAATTTTACAATCATGCTCATTTAGAATCGTTCTCTCTTTCAAAAGAATGCCGAAAATTCAGACATAACTATCAGCTTGTGATTTGAGTTTGTGGCCAAGTTGAATAAGCAGTGGCTCTCTTGTTTGATTTTTACCAAACTACAAATAAATAGGAAGTTCAAAAAAGGAAATTGAAAATGAAGAAACTTGTTTGTTGTTAATGTTAACATTAATACAGCTAGATACATTATATCTGGATTCCTCTAAGTGGTTTTAGGCTAACTAGTTTGTTTCTTTTTAATATATTCAAACGTTGACAGAAATTGTAAACCTAATTTAAATATTAATAACTTCTCGGTCAAAAGGTTCAAATCTTGACCCTACATTTGTAATATTATATTGACTAGAGTTTTCCGGTGGCTGTTGCAGGGCCTAATGAGTTCGCAGCAATATGGAGTTGTCTTGGCCAATGGCGTGTAAGTAATGTTTCTGGCTATTTATTCTGTGATTACTTTCTTTCTTCTCCATTTTGAATGCATAATTTTTCTGTTTTTGCTGAAACTTATGTAAGAAAGATGATGCCAATGAAATTAGAACTTCTAACTTAAAGTAGACAATTGAGTTCAAGTTCCATTATGAACTGCTTTATGGATATAAAGAGTTGGCGGACATATTGTTGATCAGCTCGTGACCGAGTGTTCGAATTAAAACCATTAAACCATTTTAAGACACCTGAAACGTATTTTAGTTGATCCATTAAGTTGTTGGCATCTGATGTCTATGTAATATCTCTAATAAGAATTTTAACTCATATGATATATCTTAGTTCAAAACTTGGTTTCTTTCCCATTTTGTTTCTTCATTAAAAAAGAAAAACTCTCTTGCTTCTATAGCTACTGGTGTTAGCACATGCAACATGCAACATGCATCACTTCTCTTCAAGTTGATGGTTCTTTTTCAAATTCTAAAGATCTTTTTAGGACAAGATTTTCTAGATGTTTGGGAAAAAGGTTAGATAAGTAGAAAACGGAGCAACAAAGCAACCTTTTGATCCAATAATTCAGAGTCGCTCGGTGGTTTAATATAGAACGTCCTTATTATGACATTTTTATGTTGTATACAGAACAAGTTTTTTGTTTGCCTTTTCCCCATCGAAAATGAAAATTTATTTTTGGTGATTTGTTTCTTTTATGATTATCCATATTGTACCCAGTGAGGCTAAGAACGAATATCTAATTTGCAGGCCACATTTGAGAGATATGATAGAGATAGAAGTGGGAAGATTGATGCATTGGAAATGAGGGATGCACTCTATGGCCTTGGCTATGCAGTTCCATCTTCCGTCCTTCAACTTCTAAGTTCGCTATACGATGATCATTGCGGCGGTCGAGTTGAATATAATTTTGACAGTTTTGTTGAGTAAGTGCTTCAAAATAGTTGAATAGTTCTAGCCTTTTATGATCCAAAAGCCTTTGGTACTTACATTTTTGGTTTTGTGTTTCATTGCAGATGTGGCATGATAGTAAAGGTTAGATTGCTGACCTTTTACTCCCTGTTTTGAAATACGACTCCTCTAATACCGTCACGTTAACTCGACCATATAATACTATAGATACATTCTCATCGAAGTATCCAGATACGTCTTCAAATGAAACCTCTGGCATCAGTAGTTTAAAATTTAAATATCATATTCTTAGTAACAGGCACATACTTTCAAGTGTAATATGTGATGTAGAAGACTGATACCGACCCTCCTTCTAAACCAATTTTTACAAAATTTCAGGGCCTGACCGAGAAATTCAAGGAGAAGGATAGGAACTACACCGGTTCTGCAACGCTTACATACGAAGATTTCATGTCAACGATCCTCCCTTTCCTCGTATCGTACAGTTAAAAGCCCCGAAAGGTGAAAAACGTCTCTTCATTGGTTTGTTTTCTTGAAGTAGTGGAAGCTGTACATTCAGGGAAGCTGCTGTTTTAGACCAAAAAGTTCATATATGTTGGCATGGGAGGGTCTTCCCTGTACAGATTAATTTGTTTGTTTATTTGTTCTTCTGTTCTGATTCTGTCTGCTGAGTAGAAGTCTTTTATTTGTTGGTTTTTTTTAGGTTATTTGTGACTCATTTCAATCAAGTTTCGGAGCTTGTACAAGAAGATAAAGAAATATGTTGCTATGATTTATTTGAGAGAGTTGATTTTCTGTGCAACCTCACTCTCACTACTCTATAGTCTCTCTCTGTTAGTGGAGCTTAGTTCAGTTCATTTATTCTTAAAGTTTATTTTGAAGTTTAATTTTGTGTTAAAGGTGTCGTTTTTAATCACCCTTGGAGGATTGGCAACTCTCATTCTTCAGATTTCTTCAGAAGTTGCATTTGTTGTCAATTGTGTTTCTAATGATTTCTATTAGTATATAGAAGGAAAAAGAATAATGGTTAGTACCAAAAAAAAGTTGCAATATTTATCCAAGTTTAAGGTTGAATCATCTCGATTCAATTGCTAAGTTCAGG

mRNA sequence

TATTAATTGGTTTAAAATATCGAAATCTCCATTTTGAATATTGAAGCACGTTTTTTGAGGAATTGATCGGCCGAAGATTTGAAGAAATGGACGCGAAACAAACGAACAGCAGAAAGAAATCAAACCCGTGATCTCATCTTTCATTGAATCGAAGCAACCGCGTCTTCTTTCTACAGAATTCGTCTTTGATTTTTCCTTTTACTTCCCTCTGTTGCATCAATTTTATCTTTTTTCTCTGTCTTTCTCCCATCTTTTCCGTTTAAATTCCCACGCACGAAACCAAAAGTTGCTCCCCAGATTCGAATCCCCTTCTGATTCAATTTCTTGTTCTAACCCATCTTCTAATTCTCTCTTGCTCTTCTTGTTGGCCATCAATGGCGTCCTTCTTTGGAAGATTTACTTCCCATTCCCATTCCAATTCCAATGCTCCATCCGCTCCATCCGCGCCGTCACCACCGCCCGCGCCGGAAACCCACGACCAGGGCCAATCTCACGGGACCGCCGCGCAGTATTACTCCGAACAGCAGCGGCCGCGGCCTTATGGGTCTGATTATGGCGGGGTTTCTTCATATGGGTCCTACGGTTTTCCGCCGGGGACAAGCCCTGAGGTCATTAGGAGCTTTCAAATGGTGGATAGGGATGGAAGTGGATTCATCGATGAGAATGAATTGCAGCAAGCCCTTTCTTCTGGGTACCAGAGGTTTAGTCTTAGAACCGTTCGTTTGCTCATCTTCTTATTCAGAAATCCGGTTGATTCCACCAGAATGGGGCCTAATGAGTTCGCAGCAATATGGAGTTGTCTTGGCCAATGGCGTGCCACATTTGAGAGATATGATAGAGATAGAAGTGGGAAGATTGATGCATTGGAAATGAGGGATGCACTCTATGGCCTTGGCTATGCAGTTCCATCTTCCGTCCTTCAACTTCTAAGTTCGCTATACGATGATCATTGCGGCGGTCGAGTTGAATATAATTTTGACAGTTTTGTTGAATGTGGCATGATAGTAAAGGGCCTGACCGAGAAATTCAAGGAGAAGGATAGGAACTACACCGGTTCTGCAACGCTTACATACGAAGATTTCATGTCAACGATCCTCCCTTTCCTCGTATCGTACAGTTAAAAGCCCCGAAAGGTTATTTGTGACTCATTTCAATCAAGTTTCGGAGCTTGTACAAGAAGATAAAGAAATATGTTGCTATGATTTATTTGAGAGAGTTGATTTTCTGTGCAACCTCACTCTCACTACTCTATAGTCTCTCTCTGTTAGTGGAGCTTAGTTCAGTTCATTTATTCTTAAAGTTTATTTTGAAGTTTAATTTTGTGTTAAAGGTGTCGTTTTTAATCACCCTTGGAGGATTGGCAACTCTCATTCTTCAGATTTCTTCAGAAGTTGCATTTGTTGTCAATTGTGTTTCTAATGATTTCTATTAGTATATAGAAGGAAAAAGAATAATGGTTAGTACCAAAAAAAAGTTGCAATATTTATCCAAGTTTAAGGTTGAATCATCTCGATTCAATTGCTAAGTTCAGG

Coding sequence (CDS)

ATGGCGTCCTTCTTTGGAAGATTTACTTCCCATTCCCATTCCAATTCCAATGCTCCATCCGCTCCATCCGCGCCGTCACCACCGCCCGCGCCGGAAACCCACGACCAGGGCCAATCTCACGGGACCGCCGCGCAGTATTACTCCGAACAGCAGCGGCCGCGGCCTTATGGGTCTGATTATGGCGGGGTTTCTTCATATGGGTCCTACGGTTTTCCGCCGGGGACAAGCCCTGAGGTCATTAGGAGCTTTCAAATGGTGGATAGGGATGGAAGTGGATTCATCGATGAGAATGAATTGCAGCAAGCCCTTTCTTCTGGGTACCAGAGGTTTAGTCTTAGAACCGTTCGTTTGCTCATCTTCTTATTCAGAAATCCGGTTGATTCCACCAGAATGGGGCCTAATGAGTTCGCAGCAATATGGAGTTGTCTTGGCCAATGGCGTGCCACATTTGAGAGATATGATAGAGATAGAAGTGGGAAGATTGATGCATTGGAAATGAGGGATGCACTCTATGGCCTTGGCTATGCAGTTCCATCTTCCGTCCTTCAACTTCTAAGTTCGCTATACGATGATCATTGCGGCGGTCGAGTTGAATATAATTTTGACAGTTTTGTTGAATGTGGCATGATAGTAAAGGGCCTGACCGAGAAATTCAAGGAGAAGGATAGGAACTACACCGGTTCTGCAACGCTTACATACGAAGATTTCATGTCAACGATCCTCCCTTTCCTCGTATCGTACAGTTAA

Protein sequence

MASFFGRFTSHSHSNSNAPSAPSAPSPPPAPETHDQGQSHGTAAQYYSEQQRPRPYGSDYGGVSSYGSYGFPPGTSPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTVRLLIFLFRNPVDSTRMGPNEFAAIWSCLGQWRATFERYDRDRSGKIDALEMRDALYGLGYAVPSSVLQLLSSLYDDHCGGRVEYNFDSFVECGMIVKGLTEKFKEKDRNYTGSATLTYEDFMSTILPFLVSYS

Homology

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BLAST of MC03g0424 vs. ExPASy Swiss-Prot
Match: Q9ZQH1 (Probable calcium-binding protein CML48 OS=Arabidopsis thaliana OX=3702 GN=CML48 PE=2 SV=2)

HSP 1 Score: 226.9 bits (577), Expect = 2.6e-58
Identity = 120/239 (50.21%), Postives = 162/239 (67.78%), Query Frame = 0


Query: 12  SHSNSNAPSAPSAPSPPPAPETHDQGQSHGTAAQYYS-EQQRPRPYGSDYGGVSSYGSYG 71
           S+SN+ APSAP        PE+  Q Q  G +   Y+    +P    S Y G+       
Sbjct: 2   SYSNAYAPSAPE------LPESFVQQQHDGESRYTYAYPSYQPTQQFSSYSGM------- 61

Query: 72  FPPGTSPEVIRSFQMVDRDGSGFIDENELQQALS-SGYQRFSLRTVRLLIFLFRNPVDS- 131
           F P T PE++RSF+  DR+ SGF++E+EL+QALS SGY   S RT+RLL+F+++ PVDS 
Sbjct: 62  FSPETHPEIVRSFESADRNRSGFLEESELRQALSLSGYDGISNRTIRLLLFIYKIPVDSL 121

Query: 132 TRMGPNEFAAIWSCLGQWRATFERYDRDRSGKIDALEMRDALYGLGYAVPSSVLQLLSSL 191
            R+GP E+  +W+CL QWRA F RYDRDRSGK+++ ++RDA Y LG  +P+SV QL+ S 
Sbjct: 122 LRLGPKEYVELWNCLAQWRAIFNRYDRDRSGKMNSTQLRDAFYNLGCVLPTSVHQLIVSQ 181

Query: 192 YDDHCGGRVEYNFDSFVECGMIVKGLTEKFKEKDRNYTGSATLTYEDFMSTILPFLVSY 248
           +DD  G  V+  FDSF+ECGMIVKGLTEKF+E D  YTG ATL+Y+ FM  ++PF+ +Y
Sbjct: 182 FDDGTGKTVDLCFDSFLECGMIVKGLTEKFRENDPGYTGYATLSYDVFMLMVIPFIATY 227

[Close]

BLAST of MC03g0424 vs. ExPASy Swiss-Prot
Match: Q2QY10 (Calcium-binding protein CBP OS=Oryza sativa subsp. japonica OX=39947 GN=CBP PE=2 SV=1)

HSP 1 Score: 210.3 bits (534), Expect = 2.5e-53
Identity = 117/245 (47.76%), Postives = 152/245 (62.04%), Query Frame = 0


Query: 14  SNSNAP--SAPSAPSPPPAPETHDQGQSHG---TAAQYYSEQQRPRPYGSDYGGVSSYGS 73
           S+S+AP          PP+ + +  G  +G   ++  Y +    P P  + YG    YGS
Sbjct: 49  SSSSAPYYGGGGGYGAPPSTQPYGSGGGYGAPPSSQPYGAPYGAPPPSSAPYGAPGGYGS 108

Query: 74  -------YGFPPGTSPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTVRLLIFL 133
                    FPPGT P V+  FQ  DRDGSG ID+ ELQ ALS   Q FSLRTV LL++L
Sbjct: 109 PFASLVPSAFPPGTDPNVVACFQAADRDGSGMIDDKELQSALSGYSQSFSLRTVHLLMYL 168

Query: 134 FRNPVDSTRMGPNEFAAIWSCLGQWRATFERYDRDRSGKIDALEMRDALYGLGYAVPSSV 193
           F N  +  ++GP EF +++  L  WR+ FER+DRDRSGKIDA E+RDAL  LGY+V  +V
Sbjct: 169 FTN-TNVRKIGPKEFTSVFYSLQNWRSIFERFDRDRSGKIDATELRDALLSLGYSVSPTV 228

Query: 194 LQLLSSLYDDHCGGRVEYNFDSFVECGMIVKGLTEKFKEKDRNYTGSATLTYEDFMSTIL 247
           L LL S +D   G      +D+F+EC + VKGLTEKFKEKD  ++GSAT TYE FM T+L
Sbjct: 229 LDLLVSKFDKTGGKNKAIEYDNFIECCLTVKGLTEKFKEKDTAFSGSATFTYEAFMLTVL 288

[Close]

BLAST of MC03g0424 vs. ExPASy Swiss-Prot
Match: Q9FYE4 (Probable calcium-binding protein CML50 OS=Arabidopsis thaliana OX=3702 GN=CML50 PE=2 SV=1)

HSP 1 Score: 203.4 bits (516), Expect = 3.0e-51
Identity = 124/281 (44.13%), Postives = 157/281 (55.87%), Query Frame = 0


Query: 19  PSAPSAPSP-----PPAPETHDQGQS---HGTAAQYYSEQQRPRP-----YGSDYG---- 78
           PSAP APSP     PP  + +  G        ++ Y S    PRP     +G  YG    
Sbjct: 75  PSAPYAPSPGDYNKPPKEKPYGGGYGAPPPSGSSDYGSYGAGPRPSQPSGHGGGYGATPP 134

Query: 79  -GVSSYGSY-----------------------------------GFPPGTSPEVIRSFQM 138
            GVS YGSY                                   GF PGT P ++  FQ 
Sbjct: 135 HGVSDYGSYGGAPPRPASSGHGGGYGGYPPQASYGSPFASLIPSGFAPGTDPNIVACFQA 194

Query: 139 VDRDGSGFIDENELQQALSSGYQRFSLRTVRLLIFLFRNPVDSTRMGPNEFAAIWSCLGQ 198
            D+DGSGFID+ ELQ ALSS  QRFS+RTV LL++LF N  ++ ++GP EF A++  L  
Sbjct: 195 ADQDGSGFIDDKELQGALSSYQQRFSMRTVHLLMYLFTNS-NAMKIGPKEFTALFYSLQN 254

Query: 199 WRATFERYDRDRSGKIDALEMRDALYGLGYAVPSSVLQLLSSLYDDHCGGRVEYNFDSFV 247
           WR+ FER D+DRSG+ID  E+RDAL  LG++V   VL LL S +D   G      +D+F+
Sbjct: 255 WRSIFERSDKDRSGRIDVNELRDALLSLGFSVSPVVLDLLVSKFDKSGGKNRAIEYDNFI 314

[Close]

BLAST of MC03g0424 vs. ExPASy Swiss-Prot
Match: Q8W4L0 (Probable calcium-binding protein CML49 OS=Arabidopsis thaliana OX=3702 GN=CML49 PE=2 SV=1)

HSP 1 Score: 191.0 bits (484), Expect = 1.6e-47
Identity = 109/249 (43.78%), Postives = 142/249 (57.03%), Query Frame = 0


Query: 13  HSNSNAPSAPSAPSPPPAPETHDQGQSHGTAAQY-----YSEQQRPRPYGSDYGGVSSYG 72
           H  S    +P      P+    D G   G   Q      Y    +   +G  YG      
Sbjct: 88  HGASYGSPSPGGYGAHPSSGPSDYGGYGGAPQQSGHGGGYGGAPQQSGHGGGYGAPPPQA 147

Query: 73  SYG----------FPPGTSPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTVRL 132
           SYG          FPPGT P ++  FQ  DRD SGFID+ ELQ ALSS  Q FS+RTV L
Sbjct: 148 SYGSPFASLVPSAFPPGTDPNIVACFQAADRDNSGFIDDKELQGALSSYNQSFSIRTVHL 207

Query: 133 LIFLFRNPVDSTRMGPNEFAAIWSCLGQWRATFERYDRDRSGKIDALEMRDALYGLGYAV 192
           L++LF N  +  ++GP EF +++  L  WR+ FER+D+DRSG+ID  E+RDAL  LG++V
Sbjct: 208 LMYLFTNS-NVRKIGPKEFTSLFFSLQNWRSIFERFDKDRSGRIDTNELRDALMSLGFSV 267

Query: 193 PSSVLQLLSSLYDDHCGGRVEYNFDSFVECGMIVKGLTEKFKEKDRNYTGSATLTYEDFM 247
              +L LL S +D   G      +D+F+EC + VKGLTEKFKEKD   +GSA   YE+FM
Sbjct: 268 SPVILDLLVSKFDKSGGRNRAIEYDNFIECCLTVKGLTEKFKEKDTALSGSAIFNYENFM 327

[Close]

BLAST of MC03g0424 vs. ExPASy Swiss-Prot
Match: Q6DC93 (Peflin OS=Danio rerio OX=7955 GN=pef1 PE=2 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 1.8e-27
Identity = 85/264 (32.20%), Postives = 125/264 (47.35%), Query Frame = 0


Query: 20  SAPSAPSPPPAPETHDQGQSHGTAAQYYSEQQRPRPYGSDYGGVSSYGSYG--------- 79
           +AP    PP AP       + G AA  Y       P G  YGG S YGSYG         
Sbjct: 15  NAPQWQQPPRAP------YAGGPAAGQYGSPYGSAPPGQQYGGGSPYGSYGQPGPRAPYG 74

Query: 80  ----------------------------FPPGTSPEVIRSFQMVDRDGSGFIDENELQQA 139
                                        PPG +PE  + F  VD D SG+I+  EL+QA
Sbjct: 75  GGQAPGGPYGGYGQPQGGPYRQQGSAGNVPPGVNPEAYQWFSTVDSDQSGYINAKELKQA 134

Query: 140 LSS-GYQRFSLRTVRLLIFLFRNPVDSTRMGPNEFAAIWSCLGQWRATFERYDRDRSGKI 199
           L +     F+  T  +++ +F +   S R+    F+A+W+ L QWRA F+++DRDRSG I
Sbjct: 135 LMNFNNSSFNDETCIMMLNMF-DKTKSGRVDVFGFSALWTFLQQWRAAFQQFDRDRSGSI 194

Query: 200 DALEMRDALYGLGYAVPSSVLQLLSSLYDDHCGGRVEYNFDSFVECGMIVKGLTEKFKEK 246
           +  EM  AL  +GY +    +Q L + Y    GG      D F++    ++ +T+ F+EK
Sbjct: 195 NTNEMHQALSQMGYNLSPQFIQELVNRYSVR-GGTGVLQLDRFIQVCTQLQSMTQAFREK 254

[Close]

BLAST of MC03g0424 vs. NCBI nr
Match: XP_022142689.1 (probable calcium-binding protein CML48 [Momordica charantia] >XP_022142690.1 probable calcium-binding protein CML48 [Momordica charantia])

HSP 1 Score: 507 bits (1305), Expect = 3.02e-181
Identity = 248/248 (100.00%), Postives = 248/248 (100.00%), Query Frame = 0


Query: 1   MASFFGRFTSHSHSNSNAPSAPSAPSPPPAPETHDQGQSHGTAAQYYSEQQRPRPYGSDY 60
           MASFFGRFTSHSHSNSNAPSAPSAPSPPPAPETHDQGQSHGTAAQYYSEQQRPRPYGSDY
Sbjct: 1   MASFFGRFTSHSHSNSNAPSAPSAPSPPPAPETHDQGQSHGTAAQYYSEQQRPRPYGSDY 60

Query: 61  GGVSSYGSYGFPPGTSPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTVRLLIF 120
           GGVSSYGSYGFPPGTSPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTVRLLIF
Sbjct: 61  GGVSSYGSYGFPPGTSPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTVRLLIF 120

Query: 121 LFRNPVDSTRMGPNEFAAIWSCLGQWRATFERYDRDRSGKIDALEMRDALYGLGYAVPSS 180
           LFRNPVDSTRMGPNEFAAIWSCLGQWRATFERYDRDRSGKIDALEMRDALYGLGYAVPSS
Sbjct: 121 LFRNPVDSTRMGPNEFAAIWSCLGQWRATFERYDRDRSGKIDALEMRDALYGLGYAVPSS 180

Query: 181 VLQLLSSLYDDHCGGRVEYNFDSFVECGMIVKGLTEKFKEKDRNYTGSATLTYEDFMSTI 240
           VLQLLSSLYDDHCGGRVEYNFDSFVECGMIVKGLTEKFKEKDRNYTGSATLTYEDFMSTI
Sbjct: 181 VLQLLSSLYDDHCGGRVEYNFDSFVECGMIVKGLTEKFKEKDRNYTGSATLTYEDFMSTI 240

Query: 241 LPFLVSYS 248
           LPFLVSYS
Sbjct: 241 LPFLVSYS 248

[Close]

BLAST of MC03g0424 vs. NCBI nr
Match: XP_038895549.1 (probable calcium-binding protein CML48 [Benincasa hispida])

HSP 1 Score: 434 bits (1115), Expect = 3.09e-152
Identity = 217/251 (86.45%), Postives = 230/251 (91.63%), Query Frame = 0


Query: 1   MASFFGRFTSHSHSNSNAPS---APSAPSPPPAPETHDQGQSHGTAAQYYSEQQRPRPYG 60
           MASFF RFTS+S+SNSN+ S   APSAPS P APET     SH TA+ YYSEQ RP+PYG
Sbjct: 1   MASFFNRFTSYSNSNSNSNSNSHAPSAPSVPSAPETQGHEHSHATASPYYSEQHRPQPYG 60

Query: 61  SDYGGVSSYGSYGFPPGTSPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTVRL 120
           S+YGGVSSYGSYGFPPGTSPEVIRSFQMVDRDGSGFIDENELQQALSSGYQ+FSLRTVRL
Sbjct: 61  SNYGGVSSYGSYGFPPGTSPEVIRSFQMVDRDGSGFIDENELQQALSSGYQKFSLRTVRL 120

Query: 121 LIFLFRNPVDSTRMGPNEFAAIWSCLGQWRATFERYDRDRSGKIDALEMRDALYGLGYAV 180
           LIFLFRNPVDS+RMGPNEFAA+W+CLGQWR  FERYDRDRSG+IDALEMRDALYGLGYAV
Sbjct: 121 LIFLFRNPVDSSRMGPNEFAALWNCLGQWRGMFERYDRDRSGRIDALEMRDALYGLGYAV 180

Query: 181 PSSVLQLLSSLYDDHCGGRVEYNFDSFVECGMIVKGLTEKFKEKDRNYTGSATLTYEDFM 240
           PSSVLQLL SLYDD  G RVE+NFDSFVECGMIVKGLTEKFKEKDR+YTGSATLTYEDFM
Sbjct: 181 PSSVLQLLISLYDDRSGERVEFNFDSFVECGMIVKGLTEKFKEKDRHYTGSATLTYEDFM 240

Query: 241 STILPFLVSYS 248
           STILPFLVSYS
Sbjct: 241 STILPFLVSYS 251

[Close]

BLAST of MC03g0424 vs. NCBI nr
Match: KAG6583656.1 (putative calcium-binding protein CML48, partial [Cucurbita argyrosperma subsp. sororia] >KAG7028448.1 putative calcium-binding protein CML48 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 433 bits (1114), Expect = 4.08e-152
Identity = 215/249 (86.35%), Postives = 227/249 (91.16%), Query Frame = 0


Query: 1   MASFFGRFTSHSHSNSNA-PSAPSAPSPPPAPETHDQGQSHGTAAQYYSEQQRPRPYGSD 60
           MASFFGRFTSHS S+SN+ P APSAP  P APE H    SHGTA+ Y+SE   P+PYGS+
Sbjct: 1   MASFFGRFTSHSSSSSNSKPHAPSAPYAPSAPEVHGHEHSHGTASPYFSEHHPPQPYGSN 60

Query: 61  YGGVSSYGSYGFPPGTSPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTVRLLI 120
           YGGVSSYGSYGFPPGT PEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTVRLLI
Sbjct: 61  YGGVSSYGSYGFPPGTPPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTVRLLI 120

Query: 121 FLFRNPVDSTRMGPNEFAAIWSCLGQWRATFERYDRDRSGKIDALEMRDALYGLGYAVPS 180
           FLFRNPVDS+RMGPNEF A+W+CLGQWR TFERYDRDRSGKIDALEMRDALYGLGYAVPS
Sbjct: 121 FLFRNPVDSSRMGPNEFTALWNCLGQWRGTFERYDRDRSGKIDALEMRDALYGLGYAVPS 180

Query: 181 SVLQLLSSLYDDHCGGRVEYNFDSFVECGMIVKGLTEKFKEKDRNYTGSATLTYEDFMST 240
           SVL+LL SLYDD  G RVE+NFDSFVECGMIVKGLTEKFKEKDR+YTGSATLTYEDFMST
Sbjct: 181 SVLELLISLYDDRSGRRVEFNFDSFVECGMIVKGLTEKFKEKDRHYTGSATLTYEDFMST 240

Query: 241 ILPFLVSYS 248
           ILPFLVSYS
Sbjct: 241 ILPFLVSYS 249

[Close]

BLAST of MC03g0424 vs. NCBI nr
Match: XP_022967101.1 (probable calcium-binding protein CML48 [Cucurbita maxima])

HSP 1 Score: 433 bits (1114), Expect = 4.08e-152
Identity = 213/249 (85.54%), Postives = 232/249 (93.17%), Query Frame = 0


Query: 1   MASFFGRFTSHSHSNSNAPS-APSAPSPPPAPETHDQGQSHGTAAQYYSEQQRPRPYGSD 60
           MASFFGRFTS+S+SNS++ S APSAPS P  PET+  G SHGTA+ YYS+Q +P+PYGS+
Sbjct: 1   MASFFGRFTSYSNSNSHSHSHAPSAPSAPSEPETYGHGNSHGTASPYYSQQHQPQPYGSN 60

Query: 61  YGGVSSYGSYGFPPGTSPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTVRLLI 120
           YGGVSSYGSYGFPPGTSPEVIRSFQMVD+D SGFIDENELQ+ALSSGYQRFSLRTVRLLI
Sbjct: 61  YGGVSSYGSYGFPPGTSPEVIRSFQMVDKDRSGFIDENELQEALSSGYQRFSLRTVRLLI 120

Query: 121 FLFRNPVDSTRMGPNEFAAIWSCLGQWRATFERYDRDRSGKIDALEMRDALYGLGYAVPS 180
           FLFRNP+DS+RMGPNEF A+W+CLGQWR TFERYDRDRSG+IDALEMRDALYGLGYAVPS
Sbjct: 121 FLFRNPIDSSRMGPNEFTALWNCLGQWRGTFERYDRDRSGRIDALEMRDALYGLGYAVPS 180

Query: 181 SVLQLLSSLYDDHCGGRVEYNFDSFVECGMIVKGLTEKFKEKDRNYTGSATLTYEDFMST 240
           SVLQLL SLYDD  G RVEYNFDSFVECGMIVKGLTEKFKEKD++YTGSATLTYEDFMST
Sbjct: 181 SVLQLLISLYDDRSGRRVEYNFDSFVECGMIVKGLTEKFKEKDKHYTGSATLTYEDFMST 240

Query: 241 ILPFLVSYS 248
           ILPFLVSYS
Sbjct: 241 ILPFLVSYS 249

[Close]

BLAST of MC03g0424 vs. NCBI nr
Match: XP_023519854.1 (probable calcium-binding protein CML48 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 433 bits (1114), Expect = 4.08e-152
Identity = 215/249 (86.35%), Postives = 227/249 (91.16%), Query Frame = 0


Query: 1   MASFFGRFTSHSHSNSNA-PSAPSAPSPPPAPETHDQGQSHGTAAQYYSEQQRPRPYGSD 60
           MASFFGRFTSHS S+SN+ P APSAP  P APE H    SHGTA+ Y+SE   P+PYGS+
Sbjct: 1   MASFFGRFTSHSSSSSNSKPHAPSAPYAPSAPEAHGHEHSHGTASPYFSENHPPQPYGSN 60

Query: 61  YGGVSSYGSYGFPPGTSPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTVRLLI 120
           YGGVSSYGSYGFPPGT PEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTVRLLI
Sbjct: 61  YGGVSSYGSYGFPPGTPPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTVRLLI 120

Query: 121 FLFRNPVDSTRMGPNEFAAIWSCLGQWRATFERYDRDRSGKIDALEMRDALYGLGYAVPS 180
           FLFRNPVDS+RMGPNEF A+W+CLGQWR TFERYDRDRSGKIDALEMRDALYGLGYAVPS
Sbjct: 121 FLFRNPVDSSRMGPNEFTALWNCLGQWRGTFERYDRDRSGKIDALEMRDALYGLGYAVPS 180

Query: 181 SVLQLLSSLYDDHCGGRVEYNFDSFVECGMIVKGLTEKFKEKDRNYTGSATLTYEDFMST 240
           SVL+LL SLYDD  G RVE+NFDSFVECGMIVKGLTEKFKEKDR+YTGSATLTYEDFMST
Sbjct: 181 SVLELLISLYDDRSGRRVEFNFDSFVECGMIVKGLTEKFKEKDRHYTGSATLTYEDFMST 240

Query: 241 ILPFLVSYS 248
           ILPFLVSYS
Sbjct: 241 ILPFLVSYS 249

[Close]

BLAST of MC03g0424 vs. ExPASy TrEMBL
Match: A0A6J1CM69 (probable calcium-binding protein CML48 OS=Momordica charantia OX=3673 GN=LOC111012744 PE=4 SV=1)

HSP 1 Score: 507 bits (1305), Expect = 1.46e-181
Identity = 248/248 (100.00%), Postives = 248/248 (100.00%), Query Frame = 0


Query: 1   MASFFGRFTSHSHSNSNAPSAPSAPSPPPAPETHDQGQSHGTAAQYYSEQQRPRPYGSDY 60
           MASFFGRFTSHSHSNSNAPSAPSAPSPPPAPETHDQGQSHGTAAQYYSEQQRPRPYGSDY
Sbjct: 1   MASFFGRFTSHSHSNSNAPSAPSAPSPPPAPETHDQGQSHGTAAQYYSEQQRPRPYGSDY 60

Query: 61  GGVSSYGSYGFPPGTSPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTVRLLIF 120
           GGVSSYGSYGFPPGTSPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTVRLLIF
Sbjct: 61  GGVSSYGSYGFPPGTSPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTVRLLIF 120

Query: 121 LFRNPVDSTRMGPNEFAAIWSCLGQWRATFERYDRDRSGKIDALEMRDALYGLGYAVPSS 180
           LFRNPVDSTRMGPNEFAAIWSCLGQWRATFERYDRDRSGKIDALEMRDALYGLGYAVPSS
Sbjct: 121 LFRNPVDSTRMGPNEFAAIWSCLGQWRATFERYDRDRSGKIDALEMRDALYGLGYAVPSS 180

Query: 181 VLQLLSSLYDDHCGGRVEYNFDSFVECGMIVKGLTEKFKEKDRNYTGSATLTYEDFMSTI 240
           VLQLLSSLYDDHCGGRVEYNFDSFVECGMIVKGLTEKFKEKDRNYTGSATLTYEDFMSTI
Sbjct: 181 VLQLLSSLYDDHCGGRVEYNFDSFVECGMIVKGLTEKFKEKDRNYTGSATLTYEDFMSTI 240

Query: 241 LPFLVSYS 248
           LPFLVSYS
Sbjct: 241 LPFLVSYS 248

[Close]

BLAST of MC03g0424 vs. ExPASy TrEMBL
Match: A0A6J1HR40 (probable calcium-binding protein CML48 OS=Cucurbita maxima OX=3661 GN=LOC111466605 PE=4 SV=1)

HSP 1 Score: 433 bits (1114), Expect = 1.98e-152
Identity = 213/249 (85.54%), Postives = 232/249 (93.17%), Query Frame = 0


Query: 1   MASFFGRFTSHSHSNSNAPS-APSAPSPPPAPETHDQGQSHGTAAQYYSEQQRPRPYGSD 60
           MASFFGRFTS+S+SNS++ S APSAPS P  PET+  G SHGTA+ YYS+Q +P+PYGS+
Sbjct: 1   MASFFGRFTSYSNSNSHSHSHAPSAPSAPSEPETYGHGNSHGTASPYYSQQHQPQPYGSN 60

Query: 61  YGGVSSYGSYGFPPGTSPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTVRLLI 120
           YGGVSSYGSYGFPPGTSPEVIRSFQMVD+D SGFIDENELQ+ALSSGYQRFSLRTVRLLI
Sbjct: 61  YGGVSSYGSYGFPPGTSPEVIRSFQMVDKDRSGFIDENELQEALSSGYQRFSLRTVRLLI 120

Query: 121 FLFRNPVDSTRMGPNEFAAIWSCLGQWRATFERYDRDRSGKIDALEMRDALYGLGYAVPS 180
           FLFRNP+DS+RMGPNEF A+W+CLGQWR TFERYDRDRSG+IDALEMRDALYGLGYAVPS
Sbjct: 121 FLFRNPIDSSRMGPNEFTALWNCLGQWRGTFERYDRDRSGRIDALEMRDALYGLGYAVPS 180

Query: 181 SVLQLLSSLYDDHCGGRVEYNFDSFVECGMIVKGLTEKFKEKDRNYTGSATLTYEDFMST 240
           SVLQLL SLYDD  G RVEYNFDSFVECGMIVKGLTEKFKEKD++YTGSATLTYEDFMST
Sbjct: 181 SVLQLLISLYDDRSGRRVEYNFDSFVECGMIVKGLTEKFKEKDKHYTGSATLTYEDFMST 240

Query: 241 ILPFLVSYS 248
           ILPFLVSYS
Sbjct: 241 ILPFLVSYS 249

[Close]

BLAST of MC03g0424 vs. ExPASy TrEMBL
Match: A0A6J1EVZ6 (probable calcium-binding protein CML48 OS=Cucurbita moschata OX=3662 GN=LOC111438517 PE=4 SV=1)

HSP 1 Score: 431 bits (1108), Expect = 1.62e-151
Identity = 214/249 (85.94%), Postives = 226/249 (90.76%), Query Frame = 0


Query: 1   MASFFGRFTSHSHSNSNA-PSAPSAPSPPPAPETHDQGQSHGTAAQYYSEQQRPRPYGSD 60
           MASFFGRFTSHS S+SN+ P APSAP  P APE H    SHGTA+ Y+SE   P+PYGS+
Sbjct: 1   MASFFGRFTSHSSSSSNSKPHAPSAPYAPSAPEVHGHEHSHGTASPYFSEHHPPQPYGSN 60

Query: 61  YGGVSSYGSYGFPPGTSPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTVRLLI 120
           YG VSSYGSYGFPPGT PEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTVRLLI
Sbjct: 61  YGAVSSYGSYGFPPGTPPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTVRLLI 120

Query: 121 FLFRNPVDSTRMGPNEFAAIWSCLGQWRATFERYDRDRSGKIDALEMRDALYGLGYAVPS 180
           FLFRNPVDS+RMGPNEF A+W+CLGQWR TFERYDRDRSGKIDALEMRDALYGLGYAVPS
Sbjct: 121 FLFRNPVDSSRMGPNEFTALWNCLGQWRGTFERYDRDRSGKIDALEMRDALYGLGYAVPS 180

Query: 181 SVLQLLSSLYDDHCGGRVEYNFDSFVECGMIVKGLTEKFKEKDRNYTGSATLTYEDFMST 240
           SVL+LL SLYDD  G RVE+NFDSFVECGMIVKGLTEKFKEKDR+YTGSATLTYEDFMST
Sbjct: 181 SVLELLISLYDDRSGRRVEFNFDSFVECGMIVKGLTEKFKEKDRHYTGSATLTYEDFMST 240

Query: 241 ILPFLVSYS 248
           ILPFLVSYS
Sbjct: 241 ILPFLVSYS 249

[Close]

BLAST of MC03g0424 vs. ExPASy TrEMBL
Match: A0A0A0LT26 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G164670 PE=4 SV=1)

HSP 1 Score: 428 bits (1100), Expect = 2.89e-150
Identity = 213/251 (84.86%), Postives = 229/251 (91.24%), Query Frame = 0


Query: 1   MASFFGRFTSHSHSNSNAPS---APSAPSPPPAPETHDQGQSHGTAAQYYSEQQRPRPYG 60
           MASFF RFTS+S+S+SN+ S   APSAPSPP APET  Q  SH TA+ Y+SEQ +P+PYG
Sbjct: 1   MASFFNRFTSYSNSSSNSNSNSHAPSAPSPPSAPETQGQEHSHATASPYHSEQNQPQPYG 60

Query: 61  SDYGGVSSYGSYGFPPGTSPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTVRL 120
            +YGGVSSYGSYGFPPGTSPEVIRSFQMVDRD SGFIDENELQQALSSGYQRFSLRTVRL
Sbjct: 61  FNYGGVSSYGSYGFPPGTSPEVIRSFQMVDRDRSGFIDENELQQALSSGYQRFSLRTVRL 120

Query: 121 LIFLFRNPVDSTRMGPNEFAAIWSCLGQWRATFERYDRDRSGKIDALEMRDALYGLGYAV 180
           LIFLFRNP+DS+RMGPNEF A+W+CLGQWR  FERYDRDRSG+IDALEMRDALYGLGYAV
Sbjct: 121 LIFLFRNPIDSSRMGPNEFTALWNCLGQWRGMFERYDRDRSGRIDALEMRDALYGLGYAV 180

Query: 181 PSSVLQLLSSLYDDHCGGRVEYNFDSFVECGMIVKGLTEKFKEKDRNYTGSATLTYEDFM 240
           PSSVLQLL SLYDD  G +VE+NFDSFVECGMIVKGLTEKFKEKDRNYTGSATLTYEDFM
Sbjct: 181 PSSVLQLLISLYDDRSGQQVEFNFDSFVECGMIVKGLTEKFKEKDRNYTGSATLTYEDFM 240

Query: 241 STILPFLVSYS 248
           STILPFLVSYS
Sbjct: 241 STILPFLVSYS 251

[Close]

BLAST of MC03g0424 vs. ExPASy TrEMBL
Match: A0A5D3E617 (Putative calcium-binding protein CML48 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G003180 PE=4 SV=1)

HSP 1 Score: 427 bits (1099), Expect = 4.40e-150
Identity = 213/253 (84.19%), Postives = 229/253 (90.51%), Query Frame = 0


Query: 1   MASFFGRFTSHSHSNSNAPS-----APSAPSPPPAPETHDQGQSHGTAAQYYSEQQRPRP 60
           MASFF RFTS+S+S+SN+ S     APSAPS P APET     SH TA+ YYSEQ +P+P
Sbjct: 1   MASFFNRFTSYSNSSSNSNSNSNSHAPSAPSAPSAPETQGHELSHATASPYYSEQHQPQP 60

Query: 61  YGSDYGGVSSYGSYGFPPGTSPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTV 120
           YGS+YGGVSSYGSYGFPPGTSPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTV
Sbjct: 61  YGSNYGGVSSYGSYGFPPGTSPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTV 120

Query: 121 RLLIFLFRNPVDSTRMGPNEFAAIWSCLGQWRATFERYDRDRSGKIDALEMRDALYGLGY 180
           RLLIFLFRNP+DS+RMGPNEF A+W+CLGQWR  FERYDRDRSG+IDALEMRDALYGLGY
Sbjct: 121 RLLIFLFRNPIDSSRMGPNEFTALWNCLGQWRGMFERYDRDRSGRIDALEMRDALYGLGY 180

Query: 181 AVPSSVLQLLSSLYDDHCGGRVEYNFDSFVECGMIVKGLTEKFKEKDRNYTGSATLTYED 240
           AVPSSVLQLL SLYDD  G +VE+NFDSFVECGMIVKGLTEKFKEKDRNYTGSATLTYED
Sbjct: 181 AVPSSVLQLLISLYDDRSGQQVEFNFDSFVECGMIVKGLTEKFKEKDRNYTGSATLTYED 240

Query: 241 FMSTILPFLVSYS 248
           FMSTILPFLVSY+
Sbjct: 241 FMSTILPFLVSYN 253

[Close]

BLAST of MC03g0424 vs. TAIR 10
Match: AT2G27480.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 226.9 bits (577), Expect = 1.8e-59
Identity = 120/239 (50.21%), Postives = 162/239 (67.78%), Query Frame = 0


Query: 12  SHSNSNAPSAPSAPSPPPAPETHDQGQSHGTAAQYYS-EQQRPRPYGSDYGGVSSYGSYG 71
           S+SN+ APSAP        PE+  Q Q  G +   Y+    +P    S Y G+       
Sbjct: 2   SYSNAYAPSAPE------LPESFVQQQHDGESRYTYAYPSYQPTQQFSSYSGM------- 61

Query: 72  FPPGTSPEVIRSFQMVDRDGSGFIDENELQQALS-SGYQRFSLRTVRLLIFLFRNPVDS- 131
           F P T PE++RSF+  DR+ SGF++E+EL+QALS SGY   S RT+RLL+F+++ PVDS 
Sbjct: 62  FSPETHPEIVRSFESADRNRSGFLEESELRQALSLSGYDGISNRTIRLLLFIYKIPVDSL 121

Query: 132 TRMGPNEFAAIWSCLGQWRATFERYDRDRSGKIDALEMRDALYGLGYAVPSSVLQLLSSL 191
            R+GP E+  +W+CL QWRA F RYDRDRSGK+++ ++RDA Y LG  +P+SV QL+ S 
Sbjct: 122 LRLGPKEYVELWNCLAQWRAIFNRYDRDRSGKMNSTQLRDAFYNLGCVLPTSVHQLIVSQ 181

Query: 192 YDDHCGGRVEYNFDSFVECGMIVKGLTEKFKEKDRNYTGSATLTYEDFMSTILPFLVSY 248
           +DD  G  V+  FDSF+ECGMIVKGLTEKF+E D  YTG ATL+Y+ FM  ++PF+ +Y
Sbjct: 182 FDDGTGKTVDLCFDSFLECGMIVKGLTEKFRENDPGYTGYATLSYDVFMLMVIPFIATY 227

[Close]

BLAST of MC03g0424 vs. TAIR 10
Match: AT5G04170.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 203.4 bits (516), Expect = 2.2e-52
Identity = 124/281 (44.13%), Postives = 157/281 (55.87%), Query Frame = 0


Query: 19  PSAPSAPSP-----PPAPETHDQGQS---HGTAAQYYSEQQRPRP-----YGSDYG---- 78
           PSAP APSP     PP  + +  G        ++ Y S    PRP     +G  YG    
Sbjct: 75  PSAPYAPSPGDYNKPPKEKPYGGGYGAPPPSGSSDYGSYGAGPRPSQPSGHGGGYGATPP 134

Query: 79  -GVSSYGSY-----------------------------------GFPPGTSPEVIRSFQM 138
            GVS YGSY                                   GF PGT P ++  FQ 
Sbjct: 135 HGVSDYGSYGGAPPRPASSGHGGGYGGYPPQASYGSPFASLIPSGFAPGTDPNIVACFQA 194

Query: 139 VDRDGSGFIDENELQQALSSGYQRFSLRTVRLLIFLFRNPVDSTRMGPNEFAAIWSCLGQ 198
            D+DGSGFID+ ELQ ALSS  QRFS+RTV LL++LF N  ++ ++GP EF A++  L  
Sbjct: 195 ADQDGSGFIDDKELQGALSSYQQRFSMRTVHLLMYLFTNS-NAMKIGPKEFTALFYSLQN 254

Query: 199 WRATFERYDRDRSGKIDALEMRDALYGLGYAVPSSVLQLLSSLYDDHCGGRVEYNFDSFV 247
           WR+ FER D+DRSG+ID  E+RDAL  LG++V   VL LL S +D   G      +D+F+
Sbjct: 255 WRSIFERSDKDRSGRIDVNELRDALLSLGFSVSPVVLDLLVSKFDKSGGKNRAIEYDNFI 314

[Close]

BLAST of MC03g0424 vs. TAIR 10
Match: AT3G10300.3 (Calcium-binding EF-hand family protein )

HSP 1 Score: 191.0 bits (484), Expect = 1.1e-48
Identity = 109/249 (43.78%), Postives = 142/249 (57.03%), Query Frame = 0


Query: 13  HSNSNAPSAPSAPSPPPAPETHDQGQSHGTAAQY-----YSEQQRPRPYGSDYGGVSSYG 72
           H  S    +P      P+    D G   G   Q      Y    +   +G  YG      
Sbjct: 88  HGASYGSPSPGGYGAHPSSGPSDYGGYGGAPQQSGHGGGYGGAPQQSGHGGGYGAPPPQA 147

Query: 73  SYG----------FPPGTSPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTVRL 132
           SYG          FPPGT P ++  FQ  DRD SGFID+ ELQ ALSS  Q FS+RTV L
Sbjct: 148 SYGSPFASLVPSAFPPGTDPNIVACFQAADRDNSGFIDDKELQGALSSYNQSFSIRTVHL 207

Query: 133 LIFLFRNPVDSTRMGPNEFAAIWSCLGQWRATFERYDRDRSGKIDALEMRDALYGLGYAV 192
           L++LF N  +  ++GP EF +++  L  WR+ FER+D+DRSG+ID  E+RDAL  LG++V
Sbjct: 208 LMYLFTNS-NVRKIGPKEFTSLFFSLQNWRSIFERFDKDRSGRIDTNELRDALMSLGFSV 267

Query: 193 PSSVLQLLSSLYDDHCGGRVEYNFDSFVECGMIVKGLTEKFKEKDRNYTGSATLTYEDFM 247
              +L LL S +D   G      +D+F+EC + VKGLTEKFKEKD   +GSA   YE+FM
Sbjct: 268 SPVILDLLVSKFDKSGGRNRAIEYDNFIECCLTVKGLTEKFKEKDTALSGSAIFNYENFM 327

[Close]

BLAST of MC03g0424 vs. TAIR 10
Match: AT3G10300.2 (Calcium-binding EF-hand family protein )

HSP 1 Score: 139.0 bits (349), Expect = 5.0e-33
Identity = 83/209 (39.71%), Postives = 111/209 (53.11%), Query Frame = 0


Query: 13  HSNSNAPSAPSAPSPPPAPETHDQGQSHGTAAQY-----YSEQQRPRPYGSDYGGVSSYG 72
           H  S    +P      P+    D G   G   Q      Y    +   +G  YG      
Sbjct: 88  HGASYGSPSPGGYGAHPSSGPSDYGGYGGAPQQSGHGGGYGGAPQQSGHGGGYGAPPPQA 147

Query: 73  SYG----------FPPGTSPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTVRL 132
           SYG          FPPGT P ++  FQ  DRD SGFID+ ELQ ALSS  Q FS+RTV L
Sbjct: 148 SYGSPFASLVPSAFPPGTDPNIVACFQAADRDNSGFIDDKELQGALSSYNQSFSIRTVHL 207

Query: 133 LIFLFRNPVDSTRMGPNEFAAIWSCLGQWRATFERYDRDRSGKIDALEMRDALYGLGYAV 192
           L++LF N  +  ++GP EF +++  L  WR+ FER+D+DRSG+ID  E+RDAL  LG++V
Sbjct: 208 LMYLFTNS-NVRKIGPKEFTSLFFSLQNWRSIFERFDKDRSGRIDTNELRDALMSLGFSV 267

Query: 193 PSSVLQLLSSLYDDHCGGRVEYNFDSFVE 207
              +L LL S +D   G      +D+F+E
Sbjct: 268 SPVILDLLVSKFDKSGGRNRAIEYDNFIE 295

[Close]

BLAST of MC03g0424 vs. TAIR 10
Match: AT3G10300.4 (Calcium-binding EF-hand family protein )

HSP 1 Score: 139.0 bits (349), Expect = 5.0e-33
Identity = 83/209 (39.71%), Postives = 111/209 (53.11%), Query Frame = 0


Query: 13  HSNSNAPSAPSAPSPPPAPETHDQGQSHGTAAQY-----YSEQQRPRPYGSDYGGVSSYG 72
           H  S    +P      P+    D G   G   Q      Y    +   +G  YG      
Sbjct: 88  HGASYGSPSPGGYGAHPSSGPSDYGGYGGAPQQSGHGGGYGGAPQQSGHGGGYGAPPPQA 147

Query: 73  SYG----------FPPGTSPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTVRL 132
           SYG          FPPGT P ++  FQ  DRD SGFID+ ELQ ALSS  Q FS+RTV L
Sbjct: 148 SYGSPFASLVPSAFPPGTDPNIVACFQAADRDNSGFIDDKELQGALSSYNQSFSIRTVHL 207

Query: 133 LIFLFRNPVDSTRMGPNEFAAIWSCLGQWRATFERYDRDRSGKIDALEMRDALYGLGYAV 192
           L++LF N  +  ++GP EF +++  L  WR+ FER+D+DRSG+ID  E+RDAL  LG++V
Sbjct: 208 LMYLFTNS-NVRKIGPKEFTSLFFSLQNWRSIFERFDKDRSGRIDTNELRDALMSLGFSV 267

Query: 193 PSSVLQLLSSLYDDHCGGRVEYNFDSFVE 207
              +L LL S +D   G      +D+F+E
Sbjct: 268 SPVILDLLVSKFDKSGGRNRAIEYDNFIE 295

The following BLAST results are available for this feature:

BLAST of MC03g0424 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZQH1	2.6e-58	50.21	Probable calcium-binding protein CML48 OS=Arabidopsis thaliana OX=3702 GN=CML48 ...	[more]
Q2QY10	2.5e-53	47.76	Calcium-binding protein CBP OS=Oryza sativa subsp. japonica OX=39947 GN=CBP PE=2...	[more]
Q9FYE4	3.0e-51	44.13	Probable calcium-binding protein CML50 OS=Arabidopsis thaliana OX=3702 GN=CML50 ...	[more]
Q8W4L0	1.6e-47	43.78	Probable calcium-binding protein CML49 OS=Arabidopsis thaliana OX=3702 GN=CML49 ...	[more]
Q6DC93	1.8e-27	32.20	Peflin OS=Danio rerio OX=7955 GN=pef1 PE=2 SV=1	[more]

BLAST of MC03g0424 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022142689.1	3.02e-181	100.00	probable calcium-binding protein CML48 [Momordica charantia] >XP_022142690.1 pro...	[more]
XP_038895549.1	3.09e-152	86.45	probable calcium-binding protein CML48 [Benincasa hispida]	[more]
KAG6583656.1	4.08e-152	86.35	putative calcium-binding protein CML48, partial [Cucurbita argyrosperma subsp. s...	[more]
XP_022967101.1	4.08e-152	85.54	probable calcium-binding protein CML48 [Cucurbita maxima]	[more]
XP_023519854.1	4.08e-152	86.35	probable calcium-binding protein CML48 [Cucurbita pepo subsp. pepo]	[more]

BLAST of MC03g0424 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CM69	1.46e-181	100.00	probable calcium-binding protein CML48 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A6J1HR40	1.98e-152	85.54	probable calcium-binding protein CML48 OS=Cucurbita maxima OX=3661 GN=LOC1114666...	[more]
A0A6J1EVZ6	1.62e-151	85.94	probable calcium-binding protein CML48 OS=Cucurbita moschata OX=3662 GN=LOC11143...	[more]
A0A0A0LT26	2.89e-150	84.86	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G164670 PE=4 SV=1	[more]
A0A5D3E617	4.40e-150	84.19	Putative calcium-binding protein CML48 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]

BLAST of MC03g0424 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G27480.1	1.8e-59	50.21	Calcium-binding EF-hand family protein	[more]
AT5G04170.1	2.2e-52	44.13	Calcium-binding EF-hand family protein	[more]
AT3G10300.3	1.1e-48	43.78	Calcium-binding EF-hand family protein	[more]
AT3G10300.2	5.0e-33	39.71	Calcium-binding EF-hand family protein	[more]
AT3G10300.4	5.0e-33	39.71	Calcium-binding EF-hand family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 78..106 e-value: 1.9E-4 score: 30.8 coord: 145..173 e-value: 0.03 score: 23.5
IPR002048	EF-hand domain	PFAM	PF13405	EF-hand_6	coord: 146..170 e-value: 2.5E-5 score: 23.7
IPR002048	EF-hand domain	PFAM	PF00036	EF-hand_1	coord: 79..105 e-value: 1.8E-6 score: 27.1
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 141..176 score: 12.644413
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 74..109 score: 12.198088
None	No IPR available	GENE3D	1.10.238.10		coord: 68..245 e-value: 3.7E-36 score: 126.4
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..20
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..45
None	No IPR available	PANTHER	PTHR46824:SF2	CALCIUM-BINDING PROTEIN CML48-RELATED	coord: 13..246
None	No IPR available	CDD	cd16180	EFh_PEF_Group_I	coord: 78..243 e-value: 2.77269E-65 score: 198.521
IPR044590	Probable calcium-binding protein CML48/49/50	PANTHER	PTHR46824	CALCIUM-BINDING PROTEIN CML48-RELATED	coord: 13..246
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 154..166
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 87..99
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 70..240

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC03g0424.1	MC03g0424.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding