MC03g0399 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC03g0399
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionArogenate dehydratase
LocationMC03: 10835370 .. 10836281 (-)
RNA-Seq ExpressionMC03g0399
SyntenyMC03g0399
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ACAGAGCTTGACCACTCTCCCGCTCCAATGGACGGTCCGAAGCCTCACGTCGCTTACCAAGGCGTTCCCGGCGCCTATTCCGAAGCCGCCGCCGGCAAAGCCTATCCTAACTGCGAAACCGTCCCCTGCGCTGAATTCGAGGCGGCGTTTGAATCGGTGGAGGATCACATCGCCGATCGAGCAGTTCTCCCCGTCGAAAACTCCCTCGGCGGATCTATTCATCGGAACTACGATCTCCTCCTCCGCCATAACCTCCACATAATCGGCGAGGAGCAAATACCGGTTCACCATTGCCTCTTGGCCTTACCAGGCGTCCGCCAAGAATCGGTAACCCGAGTCATCTCTCATCCCCAAGCCCTAGCTCAGTGCGAGCAATCGCTCACCAAACTCGGCCTCAACGCCGCGCGTGAGGCCGTCAGCGACACCGCCGGCGCGGCGGAGCAAATAGCCCTCAACAAGCTCCGCGACACGGCCGCGATCGCGAGCGCACGCGCCGCCGAGCTCTACGGCCTCGAGATCCTCGCGGACGGAATCCAGGACGATTCGAGAAACGTGACGCGATTCGTGGTGCTGGCGCGAGATCCATATCCAGATCTGGTCGATCCAGATCGGGCGTTCAAGACGAGCATCGCGTTCGCGCTGGAGAAAGGCACGGCGGCGCTGTTCGAGGTGCTGGCGGCGTTCGCGGCGAGGAACATTAGGTTGACGAAGATCGAGACGAGGCCGCACCGGAGCTGTCCGATCCGTCTGGTGGCGGCGGTGAAGCGGTTTGAGTACATGTTCTACGCGGACTTCGAGGCGTCAATGGCGGATTTGAGAGCTCAAGAGGCCATGGCGGAGTTGCAGGAATGCACTTCCTTTTTCAGGGTTTTGGGCAGCTATCCTATGGACATTCCGCCATCCTCTCCAAAC

mRNA sequence

ACAGAGCTTGACCACTCTCCCGCTCCAATGGACGGTCCGAAGCCTCACGTCGCTTACCAAGGCGTTCCCGGCGCCTATTCCGAAGCCGCCGCCGGCAAAGCCTATCCTAACTGCGAAACCGTCCCCTGCGCTGAATTCGAGGCGGCGTTTGAATCGGTGGAGGATCACATCGCCGATCGAGCAGTTCTCCCCGTCGAAAACTCCCTCGGCGGATCTATTCATCGGAACTACGATCTCCTCCTCCGCCATAACCTCCACATAATCGGCGAGGAGCAAATACCGGTTCACCATTGCCTCTTGGCCTTACCAGGCGTCCGCCAAGAATCGGTAACCCGAGTCATCTCTCATCCCCAAGCCCTAGCTCAGTGCGAGCAATCGCTCACCAAACTCGGCCTCAACGCCGCGCGTGAGGCCGTCAGCGACACCGCCGGCGCGGCGGAGCAAATAGCCCTCAACAAGCTCCGCGACACGGCCGCGATCGCGAGCGCACGCGCCGCCGAGCTCTACGGCCTCGAGATCCTCGCGGACGGAATCCAGGACGATTCGAGAAACGTGACGCGATTCGTGGTGCTGGCGCGAGATCCATATCCAGATCTGGTCGATCCAGATCGGGCGTTCAAGACGAGCATCGCGTTCGCGCTGGAGAAAGGCACGGCGGCGCTGTTCGAGGTGCTGGCGGCGTTCGCGGCGAGGAACATTAGGTTGACGAAGATCGAGACGAGGCCGCACCGGAGCTGTCCGATCCGTCTGGTGGCGGCGGTGAAGCGGTTTGAGTACATGTTCTACGCGGACTTCGAGGCGTCAATGGCGGATTTGAGAGCTCAAGAGGCCATGGCGGAGTTGCAGGAATGCACTTCCTTTTTCAGGGTTTTGGGCAGCTATCCTATGGACATTCCGCCATCCTCTCCAAAC

Coding sequence (CDS)

ACAGAGCTTGACCACTCTCCCGCTCCAATGGACGGTCCGAAGCCTCACGTCGCTTACCAAGGCGTTCCCGGCGCCTATTCCGAAGCCGCCGCCGGCAAAGCCTATCCTAACTGCGAAACCGTCCCCTGCGCTGAATTCGAGGCGGCGTTTGAATCGGTGGAGGATCACATCGCCGATCGAGCAGTTCTCCCCGTCGAAAACTCCCTCGGCGGATCTATTCATCGGAACTACGATCTCCTCCTCCGCCATAACCTCCACATAATCGGCGAGGAGCAAATACCGGTTCACCATTGCCTCTTGGCCTTACCAGGCGTCCGCCAAGAATCGGTAACCCGAGTCATCTCTCATCCCCAAGCCCTAGCTCAGTGCGAGCAATCGCTCACCAAACTCGGCCTCAACGCCGCGCGTGAGGCCGTCAGCGACACCGCCGGCGCGGCGGAGCAAATAGCCCTCAACAAGCTCCGCGACACGGCCGCGATCGCGAGCGCACGCGCCGCCGAGCTCTACGGCCTCGAGATCCTCGCGGACGGAATCCAGGACGATTCGAGAAACGTGACGCGATTCGTGGTGCTGGCGCGAGATCCATATCCAGATCTGGTCGATCCAGATCGGGCGTTCAAGACGAGCATCGCGTTCGCGCTGGAGAAAGGCACGGCGGCGCTGTTCGAGGTGCTGGCGGCGTTCGCGGCGAGGAACATTAGGTTGACGAAGATCGAGACGAGGCCGCACCGGAGCTGTCCGATCCGTCTGGTGGCGGCGGTGAAGCGGTTTGAGTACATGTTCTACGCGGACTTCGAGGCGTCAATGGCGGATTTGAGAGCTCAAGAGGCCATGGCGGAGTTGCAGGAATGCACTTCCTTTTTCAGGGTTTTGGGCAGCTATCCTATGGACATTCCGCCATCCTCTCCAAAC

Protein sequence

TELDHSPAPMDGPKPHVAYQGVPGAYSEAAAGKAYPNCETVPCAEFEAAFESVEDHIADRAVLPVENSLGGSIHRNYDLLLRHNLHIIGEEQIPVHHCLLALPGVRQESVTRVISHPQALAQCEQSLTKLGLNAAREAVSDTAGAAEQIALNKLRDTAAIASARAAELYGLEILADGIQDDSRNVTRFVVLARDPYPDLVDPDRAFKTSIAFALEKGTAALFEVLAAFAARNIRLTKIETRPHRSCPIRLVAAVKRFEYMFYADFEASMADLRAQEAMAELQECTSFFRVLGSYPMDIPPSSPN
Homology
BLAST of MC03g0399 vs. ExPASy Swiss-Prot
Match: D3U717 (Arogenate dehydratase 3 OS=Petunia hybrida OX=4102 GN=ADT3 PE=1 SV=1)

HSP 1 Score: 432.6 bits (1111), Expect = 3.8e-120
Identity = 228/309 (73.79%), Postives = 255/309 (82.52%), Query Frame = 0

Query: 1   TELDHSPAPMDGPKPHVAYQGVPGAYSEAAAGKAYPNCETVPCAEFEAAFESVEDHIADR 60
           T  D SPAPM G +  VAYQGVPGAYSEAAAGKAYP CE +PC +FE AF++VE  IADR
Sbjct: 125 TITDLSPAPMHGAQLRVAYQGVPGAYSEAAAGKAYPKCEAIPCDQFEVAFQAVELWIADR 184

Query: 61  AVLPVENSLGGSIHRNYDLLLRHNLHIIGEEQIPVHHCLLALPGVRQESVTRVISHPQAL 120
           AVLP+ENSLGGSIHRNYDLLLRH LHI+GE Q+PVHHCLLALPGVR+E +TRVISHPQAL
Sbjct: 185 AVLPIENSLGGSIHRNYDLLLRHRLHIVGEVQLPVHHCLLALPGVRKEYLTRVISHPQAL 244

Query: 121 AQCEQSLTKLGLNAAREAVSDTAGAAEQIALNKLRDTAAIASARAAELYGLEILADGIQD 180
           AQCE +LTKLGLN AREAV DTAGAAE IA N LRDTAAIAS+RAAELYGL+IL  GIQD
Sbjct: 245 AQCELTLTKLGLNVAREAVDDTAGAAEYIAANNLRDTAAIASSRAAELYGLDILEQGIQD 304

Query: 181 DSRNVTRFVVLARDPYPDLVDPDRAFKTSIAFALEKGTAALFEVLAAFAARNIRLTKIET 240
           D  NVTRFV+LAR+P   +   DR FKTSI FA +KGT+ LF+VL+AFA RNI LTKIE+
Sbjct: 305 DLSNVTRFVMLAREPI--IPRTDRPFKTSIVFAHDKGTSVLFKVLSAFAFRNISLTKIES 364

Query: 241 RPHRSCPIRL-----VAAVKRFEYMFYADFEASMADLRAQEAMAELQECTSFFRVLGSYP 300
           RPHR+ PIRL     V   K FEYMFY DFEASMAD+RAQ A+AE+QE TSF RVLGSYP
Sbjct: 365 RPHRNRPIRLVDDANVGTAKHFEYMFYVDFEASMADVRAQNALAEVQEFTSFLRVLGSYP 424

Query: 301 MDIPPSSPN 305
           MD+ P SP+
Sbjct: 425 MDMTPWSPS 431

BLAST of MC03g0399 vs. ExPASy Swiss-Prot
Match: D3U715 (Arogenate dehydratase 1 OS=Petunia hybrida OX=4102 GN=ADT1 PE=1 SV=1)

HSP 1 Score: 431.0 bits (1107), Expect = 1.1e-119
Identity = 229/309 (74.11%), Postives = 255/309 (82.52%), Query Frame = 0

Query: 1   TELDHSPAPMDGPKPHVAYQGVPGAYSEAAAGKAYPNCETVPCAEFEAAFESVEDHIADR 60
           T  D SPAPM G    VAYQGVPGAYSEAAAGKAYPNCE +PC +FE AF++VE  IADR
Sbjct: 116 TITDLSPAPMHGSTLRVAYQGVPGAYSEAAAGKAYPNCEAIPCDQFEVAFQAVELWIADR 175

Query: 61  AVLPVENSLGGSIHRNYDLLLRHNLHIIGEEQIPVHHCLLALPGVRQESVTRVISHPQAL 120
           AVLPVENSLGGSIHRNYDLLLRH LHI+GE Q+PVHHCLLALPGVR+E +TRVISHPQAL
Sbjct: 176 AVLPVENSLGGSIHRNYDLLLRHRLHIVGEVQLPVHHCLLALPGVRKEYLTRVISHPQAL 235

Query: 121 AQCEQSLTKLGLNAAREAVSDTAGAAEQIALNKLRDTAAIASARAAELYGLEILADGIQD 180
           AQCE ++TKLGLN AREAV DTAGAAE IA N LRDTAA+ASARAAELYGL+ILA+GIQD
Sbjct: 236 AQCELTITKLGLNVAREAVDDTAGAAEYIAANNLRDTAAVASARAAELYGLQILAEGIQD 295

Query: 181 DSRNVTRFVVLARDPYPDLVDPDRAFKTSIAFALEKGTAALFEVLAAFAARNIRLTKIET 240
           DS NVTRFV+LAR+P   +   DR FKTSI FA E GT  LF+VL+AFA RNI LTKIE+
Sbjct: 296 DSSNVTRFVMLAREPI--IPRMDRPFKTSIVFAHE-GTGVLFKVLSAFAFRNISLTKIES 355

Query: 241 RPHRSCPIRL-----VAAVKRFEYMFYADFEASMADLRAQEAMAELQECTSFFRVLGSYP 300
           RPHR+ PIRL     V   K FEYMFY DF+ASMAD+RAQ A+AE+QE TSF RVLGSYP
Sbjct: 356 RPHRNRPIRLVDDANVGTAKHFEYMFYVDFDASMADVRAQNALAEVQEFTSFLRVLGSYP 415

Query: 301 MDIPPSSPN 305
           MD+ P  P+
Sbjct: 416 MDMTPCCPS 421

BLAST of MC03g0399 vs. ExPASy Swiss-Prot
Match: Q9SGD6 (Arogenate dehydratase/prephenate dehydratase 6, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ADT6 PE=1 SV=1)

HSP 1 Score: 425.2 bits (1092), Expect = 6.0e-118
Identity = 225/305 (73.77%), Postives = 251/305 (82.30%), Query Frame = 0

Query: 4   DHSPAPMDGPKPHVAYQGVPGAYSEAAAGKAYPNCETVPCAEFEAAFESVEDHIADRAVL 63
           D SPAPM G    VAYQGVPGAYSEAAAGKAYPNC+ +PC +FE AF++VE  IADRAVL
Sbjct: 105 DLSPAPMHGSNLRVAYQGVPGAYSEAAAGKAYPNCQAIPCDQFEVAFQAVELWIADRAVL 164

Query: 64  PVENSLGGSIHRNYDLLLRHNLHIIGEEQIPVHHCLLALPGVRQESVTRVISHPQALAQC 123
           PVENSLGGSIHRNYDLLLRH LHI+GE Q+PVHHCLLALPGVR+E +TRVISHPQ LAQC
Sbjct: 165 PVENSLGGSIHRNYDLLLRHRLHIVGEVQLPVHHCLLALPGVRKEFLTRVISHPQGLAQC 224

Query: 124 EQSLTKLGLNAAREAVSDTAGAAEQIALNKLRDTAAIASARAAELYGLEILADGIQDDSR 183
           E +LTKLGLN AREAV DTAGAAE IA N LRDTAAIASARAAE+YGLEIL DGIQDD  
Sbjct: 225 EHTLTKLGLNVAREAVDDTAGAAEFIASNNLRDTAAIASARAAEIYGLEILEDGIQDDVS 284

Query: 184 NVTRFVVLARDPYPDLVDPDRAFKTSIAFALEKGTAALFEVLAAFAARNIRLTKIETRPH 243
           NVTRFV+LAR+P   +   DR FKTSI FA EKGT+ LF+VL+AFA R+I LTKIE+RP+
Sbjct: 285 NVTRFVMLAREPI--IPRTDRPFKTSIVFAHEKGTSVLFKVLSAFAFRDISLTKIESRPN 344

Query: 244 RSCPIRL-----VAAVKRFEYMFYADFEASMADLRAQEAMAELQECTSFFRVLGSYPMDI 303
            + PIR+     V   K FEYMFY DFEASMA+ RAQ A+AE+QE TSF RVLGSYPMD+
Sbjct: 345 HNRPIRVVDDANVGTAKHFEYMFYVDFEASMAEARAQNALAEVQEFTSFLRVLGSYPMDM 404

BLAST of MC03g0399 vs. ExPASy Swiss-Prot
Match: Q9ZUY3 (Arogenate dehydratase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ADT3 PE=1 SV=1)

HSP 1 Score: 424.5 bits (1090), Expect = 1.0e-117
Identity = 223/306 (72.88%), Postives = 251/306 (82.03%), Query Frame = 0

Query: 4   DHSPAPMDGPKPHVAYQGVPGAYSEAAAGKAYPNCETVPCAEFEAAFESVEDHIADRAVL 63
           D SPAPM G    VAYQGVPGAYSEAAAGKAYPNC+ +PC +FE AF++VE  IADRAVL
Sbjct: 110 DLSPAPMHGSNLRVAYQGVPGAYSEAAAGKAYPNCQAIPCDQFEVAFQAVELWIADRAVL 169

Query: 64  PVENSLGGSIHRNYDLLLRHNLHIIGEEQIPVHHCLLALPGVRQESVTRVISHPQALAQC 123
           PVENSLGGSIHRNYDLLLRH LHI+GE Q+PVHHCL+ALPGVR+E +TRVISHPQ LAQC
Sbjct: 170 PVENSLGGSIHRNYDLLLRHRLHIVGEVQLPVHHCLIALPGVRKEFLTRVISHPQGLAQC 229

Query: 124 EQSLTKLGLNAAREAVSDTAGAAEQIALNKLRDTAAIASARAAELYGLEILADGIQDDSR 183
           E +LTKLGLN AREAV DTAGAAE IA N +RDTAAIASARAAE+YGLEIL DGIQDD+ 
Sbjct: 230 EHTLTKLGLNVAREAVDDTAGAAEFIAANNIRDTAAIASARAAEIYGLEILEDGIQDDAS 289

Query: 184 NVTRFVVLARDPYPDLVDPDRAFKTSIAFALEKGTAALFEVLAAFAARNIRLTKIETRPH 243
           NVTRFV+LAR+P   +   DR FKTSI FA EKGT  LF+VL+AFA RNI LTKIE+RP+
Sbjct: 290 NVTRFVMLAREPI--IPRTDRPFKTSIVFAHEKGTCVLFKVLSAFAFRNISLTKIESRPN 349

Query: 244 RSCPIRL-----VAAVKRFEYMFYADFEASMADLRAQEAMAELQECTSFFRVLGSYPMDI 303
            + PIRL     V   K FEYMFY DFEASMA+ RAQ A++E+QE TSF RVLGSYPMD+
Sbjct: 350 HNVPIRLVDEANVGTAKHFEYMFYIDFEASMAESRAQNALSEVQEFTSFLRVLGSYPMDM 409

Query: 304 PPSSPN 305
              SP+
Sbjct: 410 TSWSPS 413

BLAST of MC03g0399 vs. ExPASy Swiss-Prot
Match: Q9FNJ8 (Arogenate dehydratase 5, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ADT5 PE=1 SV=1)

HSP 1 Score: 401.4 bits (1030), Expect = 9.3e-111
Identity = 217/309 (70.23%), Postives = 244/309 (78.96%), Query Frame = 0

Query: 1   TELDHSPAPMDGPKPHVAYQGVPGAYSEAAAGKAYPNCETVPCAEFEAAFESVEDHIADR 60
           T  D SPAP  G    VAYQGVPGAYSEAAAGKAYPN E +PC +F+ AF++VE  IADR
Sbjct: 112 TITDLSPAPSHGSTLRVAYQGVPGAYSEAAAGKAYPNSEAIPCDQFDVAFQAVELWIADR 171

Query: 61  AVLPVENSLGGSIHRNYDLLLRHNLHIIGEEQIPVHHCLLALPGVRQESVTRVISHPQAL 120
           AVLPVENSLGGSIHRNYDLLLRH LHI+GE QIPVHHCLLALPGVR + +TRVISHPQAL
Sbjct: 172 AVLPVENSLGGSIHRNYDLLLRHRLHIVGEVQIPVHHCLLALPGVRTDCITRVISHPQAL 231

Query: 121 AQCEQSLTKLGLNAAREAVSDTAGAAEQIALNKLRDTAAIASARAAELYGLEILADGIQD 180
           AQ E SL KL   AA EA  DTA AAE IA N L DTAA+ASARAAELYGL+ILADGIQD
Sbjct: 232 AQTEGSLNKLTPKAAIEAFHDTAAAAEYIAANNLHDTAAVASARAAELYGLQILADGIQD 291

Query: 181 DSRNVTRFVVLARDPYPDLVDPDRAFKTSIAFALE--KGTAALFEVLAAFAARNIRLTKI 240
           D+ NVTRF++LARDP   +   DR FKTSI FA +  KGT+ LF+VL+AFA RNI LTKI
Sbjct: 292 DAGNVTRFLMLARDPI--IPRTDRPFKTSIVFAAQEHKGTSVLFKVLSAFAFRNISLTKI 351

Query: 241 ETRPHRSCPIRL-----VAAVKRFEYMFYADFEASMADLRAQEAMAELQECTSFFRVLGS 300
           E+RPH++CP+R+     V   K FEY FY DFEASMA+ RAQ A+AE+QE TSF RVLGS
Sbjct: 352 ESRPHQNCPVRVVGDENVGTSKHFEYTFYVDFEASMAEARAQNALAEVQEYTSFLRVLGS 411

Query: 301 YPMDIPPSS 303
           YPMD+ P S
Sbjct: 412 YPMDMTPWS 418

BLAST of MC03g0399 vs. NCBI nr
Match: XP_038893857.1 (arogenate dehydratase 3-like [Benincasa hispida])

HSP 1 Score: 446 bits (1147), Expect = 1.66e-155
Identity = 234/291 (80.41%), Postives = 253/291 (86.94%), Query Frame = 0

Query: 10  MDGPKPHVAYQGVPGAYSEAAAGKAYPNCETVPCAEFEAAFESVEDHIADRAVLPVENSL 69
           MD  K HVAYQGVPGAYSEAA  KA PNCET+PC +FE AF+SVE+ IAD AVLP+ENSL
Sbjct: 1   MDAQKLHVAYQGVPGAYSEAAVAKACPNCETIPCDQFETAFQSVENRIADLAVLPIENSL 60

Query: 70  GGSIHRNYDLLLRHNLHIIGEEQIPVHHCLLALPGVRQESVTRVISHPQALAQCEQSLTK 129
           GGSIHRNYDLLLRH+LHIIGE Q+PVHHCLLALPGVR ES+ RVISHPQALAQCEQ+LTK
Sbjct: 61  GGSIHRNYDLLLRHSLHIIGEVQLPVHHCLLALPGVRPESIRRVISHPQALAQCEQTLTK 120

Query: 130 LGLNAAREAVSDTAGAAEQIALNKLRDTAAIASARAAELYGLEILADGIQDDSRNVTRFV 189
           LGLNAAREA  DTAGAAE IALN LRDTAAIAS RAAELYGLEILA+GIQDDSRNVTRFV
Sbjct: 121 LGLNAAREAFDDTAGAAEHIALNNLRDTAAIASTRAAELYGLEILANGIQDDSRNVTRFV 180

Query: 190 VLARDPYPDLVDP--DRAFKTSIAFALEKGTAALFEVLAAFAARNIRLTKIETRPHRSCP 249
           VLARDP    V+P  DRA KTSI FALEKGT ALF+VLAAFA RNI LTKIE+RP+RSCP
Sbjct: 181 VLARDP----VNPEADRALKTSIVFALEKGTPALFKVLAAFAVRNINLTKIESRPYRSCP 240

Query: 250 IRLVAAVKRFEYMFYADFEASMADLRAQEAMAELQECTSFFRVLGSYPMDI 298
           IR+   VKRFEY+FY DFEASMA+ R +EAMAEL+E  SFFRVLG YPMDI
Sbjct: 241 IRVAETVKRFEYVFYVDFEASMAEERGEEAMAELKEYISFFRVLGCYPMDI 287

BLAST of MC03g0399 vs. NCBI nr
Match: XP_008464196.1 (PREDICTED: arogenate dehydratase 3, chloroplastic-like, partial [Cucumis melo])

HSP 1 Score: 445 bits (1144), Expect = 1.08e-154
Identity = 231/304 (75.99%), Postives = 261/304 (85.86%), Query Frame = 0

Query: 1   TELDHSPAPMDGPKPHVAYQGVPGAYSEAAAGKAYPNCETVPCAEFEAAFESVEDHIADR 60
           T  ++SPAPM   K HVAYQGVPGAYSEAAAGKA PN  T+PC +FE AF+SVE+ IAD 
Sbjct: 13  TVGNYSPAPMGLQKLHVAYQGVPGAYSEAAAGKACPNSLTIPCHQFETAFQSVENRIADL 72

Query: 61  AVLPVENSLGGSIHRNYDLLLRHNLHIIGEEQIPVHHCLLALPGVRQESVTRVISHPQAL 120
           AVLP+ENSLGGSIHRNYDLLLRH+LHIIGE Q+PVHHCLLALPGVR ES+ R+ISHPQAL
Sbjct: 73  AVLPIENSLGGSIHRNYDLLLRHSLHIIGEVQLPVHHCLLALPGVRSESIRRIISHPQAL 132

Query: 121 AQCEQSLTKLGLNAAREAVSDTAGAAEQIALNKLRDTAAIASARAAELYGLEILADGIQD 180
           +QCEQ+LTKLGLNAAREA  DTAGAAE IALN LRDTAAIASARAAELYGLEILA+GIQD
Sbjct: 133 SQCEQTLTKLGLNAAREAFDDTAGAAEHIALNNLRDTAAIASARAAELYGLEILANGIQD 192

Query: 181 DSRNVTRFVVLARDPYPDLVDPDRAFKTSIAFALEKGTAALFEVLAAFAARNIRLTKIET 240
           DSRNVTRFVVLARDP   + +PD   KTS+ FALEKGT ALF+VLAAFA RNI LTKIE+
Sbjct: 193 DSRNVTRFVVLARDPV--IPEPDLPLKTSVVFALEKGTPALFKVLAAFAMRNISLTKIES 252

Query: 241 RPHRSCPIRLVAAVKRFEYMFYADFEASMADLRAQEAMAELQECTSFFRVLGSYPMDIPP 300
           RPHRSCP+R+   VKRFEY+FYADFEASM + + +EAMAEL+E  SFFRVLG YPM++  
Sbjct: 253 RPHRSCPMRVAETVKRFEYVFYADFEASMEEGKGEEAMAELKEYASFFRVLGCYPMEVAM 312

Query: 301 SSPN 304
           ++ N
Sbjct: 313 ATKN 314

BLAST of MC03g0399 vs. NCBI nr
Match: XP_021611125.1 (arogenate dehydratase 3, chloroplastic-like [Manihot esculenta] >OAY51458.1 hypothetical protein MANES_04G008300v8 [Manihot esculenta])

HSP 1 Score: 444 bits (1143), Expect = 7.96e-153
Identity = 233/309 (75.40%), Postives = 260/309 (84.14%), Query Frame = 0

Query: 1   TELDHSPAPMDGPKPHVAYQGVPGAYSEAAAGKAYPNCETVPCAEFEAAFESVEDHIADR 60
           T  D SPAPM G    VAYQGVPGAYSEAAAGKAYPNCE++PC +FE AF++VE  +ADR
Sbjct: 119 TITDLSPAPMHGSHLRVAYQGVPGAYSEAAAGKAYPNCESIPCDQFEVAFQAVELWVADR 178

Query: 61  AVLPVENSLGGSIHRNYDLLLRHNLHIIGEEQIPVHHCLLALPGVRQESVTRVISHPQAL 120
           AVLPVENSLGGSIHRNYDLLLRH LHI+GE Q+PVHHCLLALPGV +E++TRVISHPQAL
Sbjct: 179 AVLPVENSLGGSIHRNYDLLLRHRLHIVGEVQLPVHHCLLALPGVHKENITRVISHPQAL 238

Query: 121 AQCEQSLTKLGLNAAREAVSDTAGAAEQIALNKLRDTAAIASARAAELYGLEILADGIQD 180
           AQCE SL+KLGLNAAREAV DTAGAAE IA N LRDTAAIASARAAELYGL+ILADGIQD
Sbjct: 239 AQCELSLSKLGLNAAREAVDDTAGAAEHIAANNLRDTAAIASARAAELYGLQILADGIQD 298

Query: 181 DSRNVTRFVVLARDPYPDLVDPDRAFKTSIAFALEKGTAALFEVLAAFAARNIRLTKIET 240
           DS NVTRFV+LAR+P   +   DR FKTSI FA +KGT+ LF+VL+AFA RNI LTKIE+
Sbjct: 299 DSSNVTRFVMLAREPI--IPRTDRPFKTSIVFAHDKGTSVLFKVLSAFAFRNINLTKIES 358

Query: 241 RPHRSCPIRLV-----AAVKRFEYMFYADFEASMADLRAQEAMAELQECTSFFRVLGSYP 300
           RPHR+CPIRLV        K FEYMFY DFEASMAD+RAQ A+AE+QE TSF RVLGSYP
Sbjct: 359 RPHRNCPIRLVDDANVGTAKHFEYMFYVDFEASMADVRAQNALAEVQEFTSFLRVLGSYP 418

Query: 301 MDIPPSSPN 304
           MD+ P  P+
Sbjct: 419 MDMTPWCPS 425

BLAST of MC03g0399 vs. NCBI nr
Match: KAA0065884.1 (arogenate dehydratase 3 [Cucumis melo var. makuwa] >TYK15133.1 arogenate dehydratase 3 [Cucumis melo var. makuwa])

HSP 1 Score: 436 bits (1122), Expect = 1.14e-151
Identity = 225/291 (77.32%), Postives = 253/291 (86.94%), Query Frame = 0

Query: 14  KPHVAYQGVPGAYSEAAAGKAYPNCETVPCAEFEAAFESVEDHIADRAVLPVENSLGGSI 73
           K HVAYQGVPGAYSEAAAGKA PN  T+PC +FE AF+SVE+ IAD AVLP+ENSLGGSI
Sbjct: 5   KLHVAYQGVPGAYSEAAAGKACPNSLTIPCHQFETAFQSVENRIADLAVLPIENSLGGSI 64

Query: 74  HRNYDLLLRHNLHIIGEEQIPVHHCLLALPGVRQESVTRVISHPQALAQCEQSLTKLGLN 133
           HRNYDLLLRH+LHIIGE Q+PVHHCLLALPGVR ES+ R+ISHPQAL+QCEQ+LTKLGLN
Sbjct: 65  HRNYDLLLRHSLHIIGEVQLPVHHCLLALPGVRSESIRRIISHPQALSQCEQTLTKLGLN 124

Query: 134 AAREAVSDTAGAAEQIALNKLRDTAAIASARAAELYGLEILADGIQDDSRNVTRFVVLAR 193
           AAREA  DTAGAAE IALN LRDTAAIASARAAELYGLEILA+GIQDDSRNVTRFVVLAR
Sbjct: 125 AAREAFDDTAGAAEHIALNNLRDTAAIASARAAELYGLEILANGIQDDSRNVTRFVVLAR 184

Query: 194 DPYPDLVDPDRAFKTSIAFALEKGTAALFEVLAAFAARNIRLTKIETRPHRSCPIRLVAA 253
           DP   + +PD   KTS+ FALEKGT ALF+VLAAFA RNI LTKIE+RPHRSCP+R+   
Sbjct: 185 DPV--IPEPDLPLKTSVVFALEKGTPALFKVLAAFAMRNISLTKIESRPHRSCPMRVAET 244

Query: 254 VKRFEYMFYADFEASMADLRAQEAMAELQECTSFFRVLGSYPMDIPPSSPN 304
           VKRFEY+FYADFEASM + + +EAMAEL+E  SFFRVLG YPM++  ++ N
Sbjct: 245 VKRFEYVFYADFEASMEEGKGEEAMAELKEYASFFRVLGCYPMEVAMATKN 293

BLAST of MC03g0399 vs. NCBI nr
Match: XP_023534062.1 (arogenate dehydratase/prephenate dehydratase 6, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 440 bits (1132), Expect = 3.99e-151
Identity = 231/309 (74.76%), Postives = 257/309 (83.17%), Query Frame = 0

Query: 1   TELDHSPAPMDGPKPHVAYQGVPGAYSEAAAGKAYPNCETVPCAEFEAAFESVEDHIADR 60
           T  D SPAPM G K  VAYQGVPGAYSEAAAGKAYPNC+ +PC +FE AF++VE  IADR
Sbjct: 121 TITDFSPAPMHGEKLRVAYQGVPGAYSEAAAGKAYPNCDAIPCDQFEVAFQAVELWIADR 180

Query: 61  AVLPVENSLGGSIHRNYDLLLRHNLHIIGEEQIPVHHCLLALPGVRQESVTRVISHPQAL 120
           AVLPVENSLGGSIHRNYDLLLRH LHI+GE Q+PVHHCLLALPGVR+E + RVISHPQAL
Sbjct: 181 AVLPVENSLGGSIHRNYDLLLRHRLHIVGEVQLPVHHCLLALPGVRKEYLNRVISHPQAL 240

Query: 121 AQCEQSLTKLGLNAAREAVSDTAGAAEQIALNKLRDTAAIASARAAELYGLEILADGIQD 180
           AQCE +LTKLGLN  REAV DTAGAAE IA N L DTAAIASARAAELYGL+++ADGIQD
Sbjct: 241 AQCELTLTKLGLNVTREAVDDTAGAAEYIAANNLLDTAAIASARAAELYGLQVIADGIQD 300

Query: 181 DSRNVTRFVVLARDPYPDLVDPDRAFKTSIAFALEKGTAALFEVLAAFAARNIRLTKIET 240
           DS NVTRFV+LARDP   +   DR FKTSI FA +KGT+ALF+VL+AFA RNI LTKIE+
Sbjct: 301 DSNNVTRFVMLARDPI--IPRTDRPFKTSIVFAHDKGTSALFKVLSAFAFRNISLTKIES 360

Query: 241 RPHRSCPIRLV-----AAVKRFEYMFYADFEASMADLRAQEAMAELQECTSFFRVLGSYP 300
           RPHR+CPIRLV        K FEYMFY DFEASMA+ RAQ A+AE+QE TSF RVLGSYP
Sbjct: 361 RPHRNCPIRLVDDANVGTAKHFEYMFYVDFEASMAETRAQNALAEVQEFTSFLRVLGSYP 420

Query: 301 MDIPPSSPN 304
           MD+ P SP+
Sbjct: 421 MDMTPWSPS 427

BLAST of MC03g0399 vs. ExPASy TrEMBL
Match: A0A1S3CKX4 (Arogenate dehydratase OS=Cucumis melo OX=3656 GN=LOC103502130 PE=4 SV=1)

HSP 1 Score: 445 bits (1144), Expect = 5.23e-155
Identity = 231/304 (75.99%), Postives = 261/304 (85.86%), Query Frame = 0

Query: 1   TELDHSPAPMDGPKPHVAYQGVPGAYSEAAAGKAYPNCETVPCAEFEAAFESVEDHIADR 60
           T  ++SPAPM   K HVAYQGVPGAYSEAAAGKA PN  T+PC +FE AF+SVE+ IAD 
Sbjct: 13  TVGNYSPAPMGLQKLHVAYQGVPGAYSEAAAGKACPNSLTIPCHQFETAFQSVENRIADL 72

Query: 61  AVLPVENSLGGSIHRNYDLLLRHNLHIIGEEQIPVHHCLLALPGVRQESVTRVISHPQAL 120
           AVLP+ENSLGGSIHRNYDLLLRH+LHIIGE Q+PVHHCLLALPGVR ES+ R+ISHPQAL
Sbjct: 73  AVLPIENSLGGSIHRNYDLLLRHSLHIIGEVQLPVHHCLLALPGVRSESIRRIISHPQAL 132

Query: 121 AQCEQSLTKLGLNAAREAVSDTAGAAEQIALNKLRDTAAIASARAAELYGLEILADGIQD 180
           +QCEQ+LTKLGLNAAREA  DTAGAAE IALN LRDTAAIASARAAELYGLEILA+GIQD
Sbjct: 133 SQCEQTLTKLGLNAAREAFDDTAGAAEHIALNNLRDTAAIASARAAELYGLEILANGIQD 192

Query: 181 DSRNVTRFVVLARDPYPDLVDPDRAFKTSIAFALEKGTAALFEVLAAFAARNIRLTKIET 240
           DSRNVTRFVVLARDP   + +PD   KTS+ FALEKGT ALF+VLAAFA RNI LTKIE+
Sbjct: 193 DSRNVTRFVVLARDPV--IPEPDLPLKTSVVFALEKGTPALFKVLAAFAMRNISLTKIES 252

Query: 241 RPHRSCPIRLVAAVKRFEYMFYADFEASMADLRAQEAMAELQECTSFFRVLGSYPMDIPP 300
           RPHRSCP+R+   VKRFEY+FYADFEASM + + +EAMAEL+E  SFFRVLG YPM++  
Sbjct: 253 RPHRSCPMRVAETVKRFEYVFYADFEASMEEGKGEEAMAELKEYASFFRVLGCYPMEVAM 312

Query: 301 SSPN 304
           ++ N
Sbjct: 313 ATKN 314

BLAST of MC03g0399 vs. ExPASy TrEMBL
Match: A0A2C9VYL0 (Arogenate dehydratase OS=Manihot esculenta OX=3983 GN=MANES_04G008300 PE=4 SV=1)

HSP 1 Score: 444 bits (1143), Expect = 3.86e-153
Identity = 233/309 (75.40%), Postives = 260/309 (84.14%), Query Frame = 0

Query: 1   TELDHSPAPMDGPKPHVAYQGVPGAYSEAAAGKAYPNCETVPCAEFEAAFESVEDHIADR 60
           T  D SPAPM G    VAYQGVPGAYSEAAAGKAYPNCE++PC +FE AF++VE  +ADR
Sbjct: 119 TITDLSPAPMHGSHLRVAYQGVPGAYSEAAAGKAYPNCESIPCDQFEVAFQAVELWVADR 178

Query: 61  AVLPVENSLGGSIHRNYDLLLRHNLHIIGEEQIPVHHCLLALPGVRQESVTRVISHPQAL 120
           AVLPVENSLGGSIHRNYDLLLRH LHI+GE Q+PVHHCLLALPGV +E++TRVISHPQAL
Sbjct: 179 AVLPVENSLGGSIHRNYDLLLRHRLHIVGEVQLPVHHCLLALPGVHKENITRVISHPQAL 238

Query: 121 AQCEQSLTKLGLNAAREAVSDTAGAAEQIALNKLRDTAAIASARAAELYGLEILADGIQD 180
           AQCE SL+KLGLNAAREAV DTAGAAE IA N LRDTAAIASARAAELYGL+ILADGIQD
Sbjct: 239 AQCELSLSKLGLNAAREAVDDTAGAAEHIAANNLRDTAAIASARAAELYGLQILADGIQD 298

Query: 181 DSRNVTRFVVLARDPYPDLVDPDRAFKTSIAFALEKGTAALFEVLAAFAARNIRLTKIET 240
           DS NVTRFV+LAR+P   +   DR FKTSI FA +KGT+ LF+VL+AFA RNI LTKIE+
Sbjct: 299 DSSNVTRFVMLAREPI--IPRTDRPFKTSIVFAHDKGTSVLFKVLSAFAFRNINLTKIES 358

Query: 241 RPHRSCPIRLV-----AAVKRFEYMFYADFEASMADLRAQEAMAELQECTSFFRVLGSYP 300
           RPHR+CPIRLV        K FEYMFY DFEASMAD+RAQ A+AE+QE TSF RVLGSYP
Sbjct: 359 RPHRNCPIRLVDDANVGTAKHFEYMFYVDFEASMADVRAQNALAEVQEFTSFLRVLGSYP 418

Query: 301 MDIPPSSPN 304
           MD+ P  P+
Sbjct: 419 MDMTPWCPS 425

BLAST of MC03g0399 vs. ExPASy TrEMBL
Match: A0A5A7VF08 (Arogenate dehydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold259G00700 PE=4 SV=1)

HSP 1 Score: 436 bits (1122), Expect = 5.51e-152
Identity = 225/291 (77.32%), Postives = 253/291 (86.94%), Query Frame = 0

Query: 14  KPHVAYQGVPGAYSEAAAGKAYPNCETVPCAEFEAAFESVEDHIADRAVLPVENSLGGSI 73
           K HVAYQGVPGAYSEAAAGKA PN  T+PC +FE AF+SVE+ IAD AVLP+ENSLGGSI
Sbjct: 5   KLHVAYQGVPGAYSEAAAGKACPNSLTIPCHQFETAFQSVENRIADLAVLPIENSLGGSI 64

Query: 74  HRNYDLLLRHNLHIIGEEQIPVHHCLLALPGVRQESVTRVISHPQALAQCEQSLTKLGLN 133
           HRNYDLLLRH+LHIIGE Q+PVHHCLLALPGVR ES+ R+ISHPQAL+QCEQ+LTKLGLN
Sbjct: 65  HRNYDLLLRHSLHIIGEVQLPVHHCLLALPGVRSESIRRIISHPQALSQCEQTLTKLGLN 124

Query: 134 AAREAVSDTAGAAEQIALNKLRDTAAIASARAAELYGLEILADGIQDDSRNVTRFVVLAR 193
           AAREA  DTAGAAE IALN LRDTAAIASARAAELYGLEILA+GIQDDSRNVTRFVVLAR
Sbjct: 125 AAREAFDDTAGAAEHIALNNLRDTAAIASARAAELYGLEILANGIQDDSRNVTRFVVLAR 184

Query: 194 DPYPDLVDPDRAFKTSIAFALEKGTAALFEVLAAFAARNIRLTKIETRPHRSCPIRLVAA 253
           DP   + +PD   KTS+ FALEKGT ALF+VLAAFA RNI LTKIE+RPHRSCP+R+   
Sbjct: 185 DPV--IPEPDLPLKTSVVFALEKGTPALFKVLAAFAMRNISLTKIESRPHRSCPMRVAET 244

Query: 254 VKRFEYMFYADFEASMADLRAQEAMAELQECTSFFRVLGSYPMDIPPSSPN 304
           VKRFEY+FYADFEASM + + +EAMAEL+E  SFFRVLG YPM++  ++ N
Sbjct: 245 VKRFEYVFYADFEASMEEGKGEEAMAELKEYASFFRVLGCYPMEVAMATKN 293

BLAST of MC03g0399 vs. ExPASy TrEMBL
Match: A0A6J1K9L2 (Arogenate dehydratase OS=Cucurbita maxima OX=3661 GN=LOC111491340 PE=4 SV=1)

HSP 1 Score: 440 bits (1132), Expect = 1.93e-151
Identity = 231/309 (74.76%), Postives = 257/309 (83.17%), Query Frame = 0

Query: 1   TELDHSPAPMDGPKPHVAYQGVPGAYSEAAAGKAYPNCETVPCAEFEAAFESVEDHIADR 60
           T  D SPAPM G K  VAYQGVPGAYSEAAAGKAYPNC+ +PC +FE AF++VE  IADR
Sbjct: 121 TITDFSPAPMHGEKLRVAYQGVPGAYSEAAAGKAYPNCDAIPCDQFEVAFQAVELWIADR 180

Query: 61  AVLPVENSLGGSIHRNYDLLLRHNLHIIGEEQIPVHHCLLALPGVRQESVTRVISHPQAL 120
           AVLPVENSLGGSIHRNYDLLLRH LHI+GE Q+PVHHCLLALPGVR+E + RVISHPQAL
Sbjct: 181 AVLPVENSLGGSIHRNYDLLLRHRLHIVGEVQLPVHHCLLALPGVRKEYLNRVISHPQAL 240

Query: 121 AQCEQSLTKLGLNAAREAVSDTAGAAEQIALNKLRDTAAIASARAAELYGLEILADGIQD 180
           AQCE +LTKLGLN  REAV DTAGAAE IA N L DTAAIASARAAELYGL+++ADGIQD
Sbjct: 241 AQCELTLTKLGLNVTREAVDDTAGAAEYIAANNLLDTAAIASARAAELYGLQVIADGIQD 300

Query: 181 DSRNVTRFVVLARDPYPDLVDPDRAFKTSIAFALEKGTAALFEVLAAFAARNIRLTKIET 240
           DS NVTRFV+LARDP   +   DR FKTSI FA +KGT+ALF+VL+AFA RNI LTKIE+
Sbjct: 301 DSNNVTRFVMLARDPI--IPRTDRPFKTSIVFAHDKGTSALFKVLSAFAFRNISLTKIES 360

Query: 241 RPHRSCPIRLV-----AAVKRFEYMFYADFEASMADLRAQEAMAELQECTSFFRVLGSYP 300
           RPHR+CPIRLV        K FEYMFY DFEASMA+ RAQ A+AE+QE TSF RVLGSYP
Sbjct: 361 RPHRNCPIRLVDDANVGTAKHFEYMFYVDFEASMAETRAQNALAEVQEFTSFLRVLGSYP 420

Query: 301 MDIPPSSPN 304
           MD+ P SP+
Sbjct: 421 MDMTPWSPS 427

BLAST of MC03g0399 vs. ExPASy TrEMBL
Match: A0A6J1H347 (Arogenate dehydratase OS=Cucurbita moschata OX=3662 GN=LOC111459678 PE=4 SV=1)

HSP 1 Score: 440 bits (1132), Expect = 1.93e-151
Identity = 231/309 (74.76%), Postives = 257/309 (83.17%), Query Frame = 0

Query: 1   TELDHSPAPMDGPKPHVAYQGVPGAYSEAAAGKAYPNCETVPCAEFEAAFESVEDHIADR 60
           T  D SPAPM G K  VAYQGVPGAYSEAAAGKAYPNC+ +PC +FE AF++VE  IADR
Sbjct: 121 TITDFSPAPMHGEKLRVAYQGVPGAYSEAAAGKAYPNCDAIPCDQFEVAFQAVELWIADR 180

Query: 61  AVLPVENSLGGSIHRNYDLLLRHNLHIIGEEQIPVHHCLLALPGVRQESVTRVISHPQAL 120
           AVLPVENSLGGSIHRNYDLLLRH LHI+GE Q+PVHHCLLALPGVR+E + RVISHPQAL
Sbjct: 181 AVLPVENSLGGSIHRNYDLLLRHRLHIVGEVQLPVHHCLLALPGVRKEYLNRVISHPQAL 240

Query: 121 AQCEQSLTKLGLNAAREAVSDTAGAAEQIALNKLRDTAAIASARAAELYGLEILADGIQD 180
           AQCE +LTKLGLN  REAV DTAGAAE IA N L DTAAIASARAAELYGL+++ADGIQD
Sbjct: 241 AQCELTLTKLGLNVTREAVDDTAGAAEYIAANNLLDTAAIASARAAELYGLQVIADGIQD 300

Query: 181 DSRNVTRFVVLARDPYPDLVDPDRAFKTSIAFALEKGTAALFEVLAAFAARNIRLTKIET 240
           DS NVTRFV+LARDP   +   DR FKTSI FA +KGT+ALF+VL+AFA RNI LTKIE+
Sbjct: 301 DSNNVTRFVMLARDPI--IPRTDRPFKTSIVFAHDKGTSALFKVLSAFAFRNISLTKIES 360

Query: 241 RPHRSCPIRLV-----AAVKRFEYMFYADFEASMADLRAQEAMAELQECTSFFRVLGSYP 300
           RPHR+CPIRLV        K FEYMFY DFEASMA+ RAQ A+AE+QE TSF RVLGSYP
Sbjct: 361 RPHRNCPIRLVDDANVGTAKHFEYMFYVDFEASMAETRAQNALAEVQEFTSFLRVLGSYP 420

Query: 301 MDIPPSSPN 304
           MD+ P SP+
Sbjct: 421 MDMTPWSPS 427

BLAST of MC03g0399 vs. TAIR 10
Match: AT1G08250.1 (arogenate dehydratase 6 )

HSP 1 Score: 425.2 bits (1092), Expect = 4.3e-119
Identity = 225/305 (73.77%), Postives = 251/305 (82.30%), Query Frame = 0

Query: 4   DHSPAPMDGPKPHVAYQGVPGAYSEAAAGKAYPNCETVPCAEFEAAFESVEDHIADRAVL 63
           D SPAPM G    VAYQGVPGAYSEAAAGKAYPNC+ +PC +FE AF++VE  IADRAVL
Sbjct: 105 DLSPAPMHGSNLRVAYQGVPGAYSEAAAGKAYPNCQAIPCDQFEVAFQAVELWIADRAVL 164

Query: 64  PVENSLGGSIHRNYDLLLRHNLHIIGEEQIPVHHCLLALPGVRQESVTRVISHPQALAQC 123
           PVENSLGGSIHRNYDLLLRH LHI+GE Q+PVHHCLLALPGVR+E +TRVISHPQ LAQC
Sbjct: 165 PVENSLGGSIHRNYDLLLRHRLHIVGEVQLPVHHCLLALPGVRKEFLTRVISHPQGLAQC 224

Query: 124 EQSLTKLGLNAAREAVSDTAGAAEQIALNKLRDTAAIASARAAELYGLEILADGIQDDSR 183
           E +LTKLGLN AREAV DTAGAAE IA N LRDTAAIASARAAE+YGLEIL DGIQDD  
Sbjct: 225 EHTLTKLGLNVAREAVDDTAGAAEFIASNNLRDTAAIASARAAEIYGLEILEDGIQDDVS 284

Query: 184 NVTRFVVLARDPYPDLVDPDRAFKTSIAFALEKGTAALFEVLAAFAARNIRLTKIETRPH 243
           NVTRFV+LAR+P   +   DR FKTSI FA EKGT+ LF+VL+AFA R+I LTKIE+RP+
Sbjct: 285 NVTRFVMLAREPI--IPRTDRPFKTSIVFAHEKGTSVLFKVLSAFAFRDISLTKIESRPN 344

Query: 244 RSCPIRL-----VAAVKRFEYMFYADFEASMADLRAQEAMAELQECTSFFRVLGSYPMDI 303
            + PIR+     V   K FEYMFY DFEASMA+ RAQ A+AE+QE TSF RVLGSYPMD+
Sbjct: 345 HNRPIRVVDDANVGTAKHFEYMFYVDFEASMAEARAQNALAEVQEFTSFLRVLGSYPMDM 404

BLAST of MC03g0399 vs. TAIR 10
Match: AT2G27820.1 (prephenate dehydratase 1 )

HSP 1 Score: 424.5 bits (1090), Expect = 7.3e-119
Identity = 223/306 (72.88%), Postives = 251/306 (82.03%), Query Frame = 0

Query: 4   DHSPAPMDGPKPHVAYQGVPGAYSEAAAGKAYPNCETVPCAEFEAAFESVEDHIADRAVL 63
           D SPAPM G    VAYQGVPGAYSEAAAGKAYPNC+ +PC +FE AF++VE  IADRAVL
Sbjct: 110 DLSPAPMHGSNLRVAYQGVPGAYSEAAAGKAYPNCQAIPCDQFEVAFQAVELWIADRAVL 169

Query: 64  PVENSLGGSIHRNYDLLLRHNLHIIGEEQIPVHHCLLALPGVRQESVTRVISHPQALAQC 123
           PVENSLGGSIHRNYDLLLRH LHI+GE Q+PVHHCL+ALPGVR+E +TRVISHPQ LAQC
Sbjct: 170 PVENSLGGSIHRNYDLLLRHRLHIVGEVQLPVHHCLIALPGVRKEFLTRVISHPQGLAQC 229

Query: 124 EQSLTKLGLNAAREAVSDTAGAAEQIALNKLRDTAAIASARAAELYGLEILADGIQDDSR 183
           E +LTKLGLN AREAV DTAGAAE IA N +RDTAAIASARAAE+YGLEIL DGIQDD+ 
Sbjct: 230 EHTLTKLGLNVAREAVDDTAGAAEFIAANNIRDTAAIASARAAEIYGLEILEDGIQDDAS 289

Query: 184 NVTRFVVLARDPYPDLVDPDRAFKTSIAFALEKGTAALFEVLAAFAARNIRLTKIETRPH 243
           NVTRFV+LAR+P   +   DR FKTSI FA EKGT  LF+VL+AFA RNI LTKIE+RP+
Sbjct: 290 NVTRFVMLAREPI--IPRTDRPFKTSIVFAHEKGTCVLFKVLSAFAFRNISLTKIESRPN 349

Query: 244 RSCPIRL-----VAAVKRFEYMFYADFEASMADLRAQEAMAELQECTSFFRVLGSYPMDI 303
            + PIRL     V   K FEYMFY DFEASMA+ RAQ A++E+QE TSF RVLGSYPMD+
Sbjct: 350 HNVPIRLVDEANVGTAKHFEYMFYIDFEASMAESRAQNALSEVQEFTSFLRVLGSYPMDM 409

Query: 304 PPSSPN 305
              SP+
Sbjct: 410 TSWSPS 413

BLAST of MC03g0399 vs. TAIR 10
Match: AT5G22630.1 (arogenate dehydratase 5 )

HSP 1 Score: 401.4 bits (1030), Expect = 6.6e-112
Identity = 217/309 (70.23%), Postives = 244/309 (78.96%), Query Frame = 0

Query: 1   TELDHSPAPMDGPKPHVAYQGVPGAYSEAAAGKAYPNCETVPCAEFEAAFESVEDHIADR 60
           T  D SPAP  G    VAYQGVPGAYSEAAAGKAYPN E +PC +F+ AF++VE  IADR
Sbjct: 112 TITDLSPAPSHGSTLRVAYQGVPGAYSEAAAGKAYPNSEAIPCDQFDVAFQAVELWIADR 171

Query: 61  AVLPVENSLGGSIHRNYDLLLRHNLHIIGEEQIPVHHCLLALPGVRQESVTRVISHPQAL 120
           AVLPVENSLGGSIHRNYDLLLRH LHI+GE QIPVHHCLLALPGVR + +TRVISHPQAL
Sbjct: 172 AVLPVENSLGGSIHRNYDLLLRHRLHIVGEVQIPVHHCLLALPGVRTDCITRVISHPQAL 231

Query: 121 AQCEQSLTKLGLNAAREAVSDTAGAAEQIALNKLRDTAAIASARAAELYGLEILADGIQD 180
           AQ E SL KL   AA EA  DTA AAE IA N L DTAA+ASARAAELYGL+ILADGIQD
Sbjct: 232 AQTEGSLNKLTPKAAIEAFHDTAAAAEYIAANNLHDTAAVASARAAELYGLQILADGIQD 291

Query: 181 DSRNVTRFVVLARDPYPDLVDPDRAFKTSIAFALE--KGTAALFEVLAAFAARNIRLTKI 240
           D+ NVTRF++LARDP   +   DR FKTSI FA +  KGT+ LF+VL+AFA RNI LTKI
Sbjct: 292 DAGNVTRFLMLARDPI--IPRTDRPFKTSIVFAAQEHKGTSVLFKVLSAFAFRNISLTKI 351

Query: 241 ETRPHRSCPIRL-----VAAVKRFEYMFYADFEASMADLRAQEAMAELQECTSFFRVLGS 300
           E+RPH++CP+R+     V   K FEY FY DFEASMA+ RAQ A+AE+QE TSF RVLGS
Sbjct: 352 ESRPHQNCPVRVVGDENVGTSKHFEYTFYVDFEASMAEARAQNALAEVQEYTSFLRVLGS 411

Query: 301 YPMDIPPSS 303
           YPMD+ P S
Sbjct: 412 YPMDMTPWS 418

BLAST of MC03g0399 vs. TAIR 10
Match: AT3G44720.1 (arogenate dehydratase 4 )

HSP 1 Score: 393.7 bits (1010), Expect = 1.4e-109
Identity = 213/309 (68.93%), Postives = 244/309 (78.96%), Query Frame = 0

Query: 1   TELDHSPAPMDGPKPHVAYQGVPGAYSEAAAGKAYPNCETVPCAEFEAAFESVEDHIADR 60
           T  D SPAP+ G    VAYQGVPGAYSEAAAGKAYPNC+ +PC +F+ AF++VE  IADR
Sbjct: 111 TITDLSPAPLHGSSLRVAYQGVPGAYSEAAAGKAYPNCDAIPCDQFDVAFQAVELWIADR 170

Query: 61  AVLPVENSLGGSIHRNYDLLLRHNLHIIGEEQIPVHHCLLALPGVRQESVTRVISHPQAL 120
           AVLPVENSLGGSIHRNYDLLLRH LHI+GE QIPVHHCLLALPGVR + V+RVISHPQAL
Sbjct: 171 AVLPVENSLGGSIHRNYDLLLRHRLHIVGEVQIPVHHCLLALPGVRTDCVSRVISHPQAL 230

Query: 121 AQCEQSLTKLGLNAAREAVSDTAGAAEQIALNKLRDTAAIASARAAELYGLEILADGIQD 180
           AQ E SL  L  +AAREA  DTA AAE I+ N L DTAA+ASARAAELY L+ILADGIQD
Sbjct: 231 AQTEHSLDVLTPHAAREAFHDTAAAAEYISANDLHDTAAVASARAAELYNLQILADGIQD 290

Query: 181 DSRNVTRFVVLARDPYPDLVDPDRAFKTSIAFALE--KGTAALFEVLAAFAARNIRLTKI 240
           D  NVTRF++LAR+P   +   DR FKTSI FA +  KGT+ LF+VL+AFA R+I LTKI
Sbjct: 291 DPGNVTRFLMLAREPI--IPRTDRPFKTSIVFAAQEHKGTSVLFKVLSAFAFRDISLTKI 350

Query: 241 ETRPHRSCPIRLV-----AAVKRFEYMFYADFEASMADLRAQEAMAELQECTSFFRVLGS 300
           E+RPH + P+R+V        K FEYMFY DFEASMA+ RAQ A+AE+QE TSF RVLGS
Sbjct: 351 ESRPHHNRPLRVVGDGSFGTSKNFEYMFYVDFEASMAEPRAQNALAEVQEYTSFLRVLGS 410

Query: 301 YPMDIPPSS 303
           YPMD+ P S
Sbjct: 411 YPMDMTPWS 417

BLAST of MC03g0399 vs. TAIR 10
Match: AT3G07630.1 (arogenate dehydratase 2 )

HSP 1 Score: 346.3 bits (887), Expect = 2.5e-95
Identity = 182/287 (63.41%), Postives = 218/287 (75.96%), Query Frame = 0

Query: 11  DGPKPHVAYQGVPGAYSEAAAGKAYPNCETVPCAEFEAAFESVEDHIADRAVLPVENSLG 70
           +G +  VAYQGV GAYSE+AA KAYPNCE VPC EF+ AFE+VE  + DRAVLP+ENSLG
Sbjct: 95  NGSRVRVAYQGVRGAYSESAAEKAYPNCEAVPCEEFDTAFEAVERWLVDRAVLPIENSLG 154

Query: 71  GSIHRNYDLLLRHNLHIIGEEQIPVHHCLLALPGVRQESVTRVISHPQALAQCEQSLTKL 130
           GSIHRNYDLLLRHNLHI+GE ++ V HCLLA  GV  E + RV+SHPQALAQCE +LTKL
Sbjct: 155 GSIHRNYDLLLRHNLHIVGEVKLAVRHCLLANHGVNIEDLRRVLSHPQALAQCENTLTKL 214

Query: 131 GLNAAREAVSDTAGAAEQIALNKLRDTAAIASARAAELYGLEILADGIQDDSRNVTRFVV 190
           GL   REAV DTAGAA+QIA   L D AA+AS +AA++YGL I+A  IQDD  NVTRF++
Sbjct: 215 GL--VREAVDDTAGAAKQIAFENLNDAAAVASEKAAKIYGLNIVAKDIQDDCDNVTRFLM 274

Query: 191 LARDPYPDLVDPDRAFKTSIAFALEKGTAALFEVLAAFAARNIRLTKIETRPHRSCPIRL 250
           LAR+P   +   +R FKTSI F+LE+G   LF+ LA FA R I LTKIE+RP R  P+R 
Sbjct: 275 LAREPI--IPGTNRLFKTSIVFSLEEGPGVLFKALAVFALRQINLTKIESRPLRKHPLRA 334

Query: 251 VAAVKRFEYMFYADFEASMADLRAQEAMAELQECTSFFRVLGSYPMD 298
              +K F+Y+FY DFEASMAD  AQ A+  L+E  +F RVLGSYP+D
Sbjct: 335 SGGLKYFDYLFYVDFEASMADEVAQNALRHLEEFATFLRVLGSYPVD 377

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
D3U7173.8e-12073.79Arogenate dehydratase 3 OS=Petunia hybrida OX=4102 GN=ADT3 PE=1 SV=1[more]
D3U7151.1e-11974.11Arogenate dehydratase 1 OS=Petunia hybrida OX=4102 GN=ADT1 PE=1 SV=1[more]
Q9SGD66.0e-11873.77Arogenate dehydratase/prephenate dehydratase 6, chloroplastic OS=Arabidopsis tha... [more]
Q9ZUY31.0e-11772.88Arogenate dehydratase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ADT3 P... [more]
Q9FNJ89.3e-11170.23Arogenate dehydratase 5, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ADT5 P... [more]
Match NameE-valueIdentityDescription
XP_038893857.11.66e-15580.41arogenate dehydratase 3-like [Benincasa hispida][more]
XP_008464196.11.08e-15475.99PREDICTED: arogenate dehydratase 3, chloroplastic-like, partial [Cucumis melo][more]
XP_021611125.17.96e-15375.40arogenate dehydratase 3, chloroplastic-like [Manihot esculenta] >OAY51458.1 hypo... [more]
KAA0065884.11.14e-15177.32arogenate dehydratase 3 [Cucumis melo var. makuwa] >TYK15133.1 arogenate dehydra... [more]
XP_023534062.13.99e-15174.76arogenate dehydratase/prephenate dehydratase 6, chloroplastic-like [Cucurbita pe... [more]
Match NameE-valueIdentityDescription
A0A1S3CKX45.23e-15575.99Arogenate dehydratase OS=Cucumis melo OX=3656 GN=LOC103502130 PE=4 SV=1[more]
A0A2C9VYL03.86e-15375.40Arogenate dehydratase OS=Manihot esculenta OX=3983 GN=MANES_04G008300 PE=4 SV=1[more]
A0A5A7VF085.51e-15277.32Arogenate dehydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold25... [more]
A0A6J1K9L21.93e-15174.76Arogenate dehydratase OS=Cucurbita maxima OX=3661 GN=LOC111491340 PE=4 SV=1[more]
A0A6J1H3471.93e-15174.76Arogenate dehydratase OS=Cucurbita moschata OX=3662 GN=LOC111459678 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G08250.14.3e-11973.77arogenate dehydratase 6 [more]
AT2G27820.17.3e-11972.88prephenate dehydratase 1 [more]
AT5G22630.16.6e-11270.23arogenate dehydratase 5 [more]
AT3G44720.11.4e-10968.93arogenate dehydratase 4 [more]
AT3G07630.12.5e-9563.41arogenate dehydratase 2 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.190.10coord: 96..182
e-value: 1.7E-60
score: 205.7
NoneNo IPR availableGENE3D3.30.70.260coord: 202..298
e-value: 1.3E-28
score: 100.6
NoneNo IPR availableGENE3D3.40.190.10coord: 17..189
e-value: 1.7E-60
score: 205.7
NoneNo IPR availablePANTHERPTHR21022:SF28PREPHENATE DEHYDRATASE, ACT DOMAIN PROTEIN-RELATEDcoord: 2..303
NoneNo IPR availablePANTHERPTHR21022PREPHENATE DEHYDRATASE P PROTEINcoord: 2..303
NoneNo IPR availableCDDcd13631PBP2_Ct-PDT_likecoord: 14..195
e-value: 8.03454E-88
score: 258.495
NoneNo IPR availableCDDcd04905ACT_CM-PDTcoord: 207..295
e-value: 6.19441E-31
score: 109.126
NoneNo IPR availableSUPERFAMILY55021ACT-likecoord: 188..297
NoneNo IPR availableSUPERFAMILY53850Periplasmic binding protein-like IIcoord: 16..196
IPR008242Bifunctional P-protein, chorismate mutase/prephenate dehydratasePIRSFPIRSF001500Chor_mut_pdt_Pprcoord: 6..298
e-value: 2.0E-71
score: 238.6
IPR001086Prephenate dehydratasePFAMPF00800PDTcoord: 17..195
e-value: 4.8E-60
score: 202.5
IPR001086Prephenate dehydratasePROSITEPS51171PREPHENATE_DEHYDR_3coord: 16..193
score: 47.709072
IPR018528Prephenate dehydratase, conserved sitePROSITEPS00857PREPHENATE_DEHYDR_1coord: 169..191
IPR018528Prephenate dehydratase, conserved sitePROSITEPS00858PREPHENATE_DEHYDR_2coord: 235..242
IPR002912ACT domainPROSITEPS51671ACTcoord: 209..295
score: 9.116565

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC03g0399.1MC03g0399.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009094 L-phenylalanine biosynthetic process
cellular_component GO:0009570 chloroplast stroma
molecular_function GO:0047769 arogenate dehydratase activity
molecular_function GO:0004106 chorismate mutase activity
molecular_function GO:0004664 prephenate dehydratase activity