MC03g0310 (gene) Bitter gourd (Dali-11) v1

BLAST of MC03g0310 vs. ExPASy Swiss-Prot
Match: O22207 (Ubiquitin carboxyl-terminal hydrolase 5 OS=Arabidopsis thaliana OX=3702 GN=UBP5 PE=1 SV=2)

HSP 1 Score: 1255.7 bits (3248), Expect = 0.0e+00
Identity = 644/927 (69.47%), Postives = 743/927 (80.15%), Query Frame = 0

Query: 64  AEVSMCSSGGRTELTPEEERIMIRDIALTAEANTKEGDVFYLITQRWWQHWIEYVNQDQP 123
           AEVSM SS   T+L+PEEER+ IRDIA+ AEAN+KEGD FYLITQRWWQ WIEYVNQDQP
Sbjct: 2   AEVSMGSSSSSTDLSPEEERVFIRDIAIAAEANSKEGDTFYLITQRWWQEWIEYVNQDQP 61

Query: 124 VNANDGSSFAEIYDSFGSSMLKRPANIDNSDLIYDAASEDSSVSIEIHDTLLEGRDYVLL 183
            N NDGSS +E  DS GSS LK+P+ IDNSDLIYD++ ED S + EI +TL EGRDYVLL
Sbjct: 62  CNTNDGSSLSEHCDSPGSSTLKKPSRIDNSDLIYDSSLEDPSNTSEIIETLQEGRDYVLL 121

Query: 184 PQEVWKQLYLWYGGGPTLARKVISAGLSQTELAVEVYPLRLQLLEVGKGDRSTIRISKKE 243
           PQEVW QL  WYGGGPTLAR+VIS+GLSQTELAVEVYPLRLQLL + K D S IRISKKE
Sbjct: 122 PQEVWNQLRSWYGGGPTLARRVISSGLSQTELAVEVYPLRLQLLLMPKSDHSAIRISKKE 181

Query: 244 TIGELHRRACEIFDLNLEQVCIWDYYGHRKHALMNDMDKTLDDANIQMDQDILVEIINHD 303
           TI ELHRRACEIFDL+ E V IWDYYGH+K++LMND+DKTLDDAN+QMDQDILVE++  D
Sbjct: 182 TIRELHRRACEIFDLDSEHVRIWDYYGHQKYSLMNDLDKTLDDANLQMDQDILVEVL--D 241

Query: 304 NNNALGGC-TSYVQDNGTTDKEATSISLESSKSNLIIAGGV-SNKGASKTEVVQ-SQSLT 363
            N  L        Q+NG  D ++TSI +E SKS+L  AGG  S++ A +T  V+ SQS  
Sbjct: 242 INGTLSSAHIQSAQENGLVDGDSTSILIEPSKSSLAAAGGFSSSRNAFRTGSVEVSQSF- 301

Query: 364 SSIRESDNTYGQSGVSTRGSSCGLTGLLNLGNTCFMNSAIQCLVHTPEFARYFREDYHQE 423
                 DNTY  +GV+TRGS+ GLTGLLNLGNTCFMNSAIQCLVHTPEFA YF+EDYHQE
Sbjct: 302 ------DNTYSSTGVTTRGSTAGLTGLLNLGNTCFMNSAIQCLVHTPEFASYFQEDYHQE 361

Query: 424 INRQNPLGMVGELAMAFGDLLRKLWAPGRTSVAPRPFKAKLARFAPQFSGYNQHDSQELL 483
           IN QNPLGMVGELA+AFGDLLRKLWAPGRT +APRPFKAKLARFAPQFSGYNQHDSQELL
Sbjct: 362 INWQNPLGMVGELALAFGDLLRKLWAPGRTPIAPRPFKAKLARFAPQFSGYNQHDSQELL 421

Query: 484 AFLLDGLHEDLNRVKHKPYIKSRDADGRPDEEVADEYWANHIARNDSIIVDVCQGQYKST 543
           AFLLDGLHEDLNRVKHKPYI SRDADGRPDEEVADE+W NHIARNDSIIVDVCQGQYKST
Sbjct: 422 AFLLDGLHEDLNRVKHKPYINSRDADGRPDEEVADEFWKNHIARNDSIIVDVCQGQYKST 481

Query: 544 LVCPVCNKVSVTFDPFMYLSLPLQSTTTRTMTATLFTSDGSKLPSTLTVTVPKQGRCRDL 603
           LVCP+CNKVSVTFDPFMYLSLPLQ  TTR +T T+F+ D + LPST+TV V KQGRCRDL
Sbjct: 482 LVCPICNKVSVTFDPFMYLSLPLQFNTTRAITVTVFSCDKTALPSTITVNVSKQGRCRDL 541

Query: 604 IQALNSACLLKHSEKLLLAEVRDHLIHRFLEDPLISLSTIKDDDHIAAYKIPKLTKNTKY 663
           IQAL +AC LK SE+L LAE+R++ IHR  EDPLI LS+IKDDDH+AAYK+ K ++NT  
Sbjct: 542 IQALTNACSLKQSEELKLAEIRNNFIHRLFEDPLIPLSSIKDDDHLAAYKLSKSSENTTL 601

Query: 664 LQLVHRRQEQVTSDAHAMSGWRPFGTPLVSVVSCDDPVTKGYIQRIIKRVLSPMAKTGTL 723
           L+LV RR++Q   +  +    +P GTPL+S  SC D +TKG I  +++ +LSP  +  ++
Sbjct: 602 LRLVLRRRDQKAGERESTVQLKPCGTPLLSSASCGDALTKGKIHCLVQNMLSPFRREESV 661

Query: 724 MDTDVFDSSVSHAGEDLHPRDSSIETCTVSISSLMNKDDPKSKGAEPFKLPLQLLNKDNV 783
                 DSS+    E    R ++ E     +  L       S      KL LQL+++DN 
Sbjct: 662 GKKGNSDSSIP---ERRSARFNNTEE-EDKVGGLKKAKKSNSSDLGASKLSLQLIDEDNK 721

Query: 784 CIDLSTGE-ESVQLPPSSSVLVYIDWSQKLMKKFDTFYLENLPEVFKSGPVMKKARTEPL 843
            I+L   E E+++LP S++V +Y+DW+ +L   +D   LE+LPEV K GP  KKAR+EPL
Sbjct: 722 TINLPDNEAEAMKLPSSATVTIYLDWTPELSGMYDITCLESLPEVLKYGPTTKKARSEPL 781

Query: 844 SLYSCLESFLREEPLVPEDMWFCPQCKERRQASKKLDLWRLPEVLVIHLKRFSYSRSMKH 903
           SLY+CLE+FLREEPLVP++MWFCPQC ERRQASKKLDLWRLPEVLVIHLKRFSYSRSMKH
Sbjct: 782 SLYACLEAFLREEPLVPDEMWFCPQCNERRQASKKLDLWRLPEVLVIHLKRFSYSRSMKH 841

Query: 904 KLETFVNFPIHDFDLTNYVANKNNSQRQLYELYALTNHYGGMGSGHYTAHIKLIDENRWY 963
           KLETFVNFPIHD DLT YVANKN SQ QLYELYALTNHYGGMGSGHYTAHIKL+D++RWY
Sbjct: 842 KLETFVNFPIHDLDLTKYVANKNLSQPQLYELYALTNHYGGMGSGHYTAHIKLLDDSRWY 901

Query: 964 NFDDSHISLINEEEVKSAAAYVLFYRR 987
           NFDDSHIS INE++VKS AAYVLFYRR
Sbjct: 902 NFDDSHISHINEDDVKSGAAYVLFYRR 915

BLAST of MC03g0310 vs. ExPASy Swiss-Prot
Match: Q9ZSB5 (Ubiquitin carboxyl-terminal hydrolase 10 OS=Arabidopsis thaliana OX=3702 GN=UBP10 PE=2 SV=2)

HSP 1 Score: 851.7 bits (2199), Expect = 8.6e-246
Identity = 446/917 (48.64%), Postives = 611/917 (66.63%), Query Frame = 0

Query: 78  TPEEERIMIRDIALTAEANTKEGDVFYLITQRWWQHWIEYVNQDQPVNANDGSSFAEIYD 137
           TPEEE+ ++ ++   +E N K+G+++++I++RW+  W EYV      +AN+ S+      
Sbjct: 20  TPEEEKRIVSELTSESEDNLKQGNLYFVISKRWYTSWQEYVEN----SANECSTGE---- 79

Query: 138 SFGSSMLKRPANIDNSDLIYDAASEDSSVS-IEIHDTLLEGRDYVLLPQEVWKQLYLWYG 197
              SS   RP  IDN D+I      DS ++  ++   L+EG DYVL+P++VWK+L  WY 
Sbjct: 80  ---SSEAPRPGPIDNHDII----ESDSDINDPQLRRLLVEGEDYVLVPKQVWKRLVEWYS 139

Query: 198 GGPTLARKVISAGLSQTELAVEVYPLRLQLLEVGKGDRSTIRISKKETIGELHRRACEIF 257
           GGP + RK+I  G      +VEVYPL L L +     R+ IR+ K+ +I EL+ + C + 
Sbjct: 140 GGPPIERKLICQGFYTRSYSVEVYPLCLMLTDGRDESRTVIRLGKQASIRELYEKVCAMT 199

Query: 258 DLNLEQVCIWDYYGHRKHALMNDMD-KTLDDANIQMDQDILVEIINHDNNNALGGCTSYV 317
            +  E+  IWDY+  RK+ L++ +  K+L+++++ MDQDILVE+         G  +S  
Sbjct: 200 GVPQEKAHIWDYFDKRKNGLLDPLSYKSLEESSLHMDQDILVEVD--------GLSSSSQ 259

Query: 318 QDNGTTDKEATSISLESSKSNLIIAGGVSNKGASKTEVVQSQSLTSSIRESDNTYGQSGV 377
               +T  E   + LE S+S + IAGG +      T    + SL   I   D+      +
Sbjct: 260 SAMSSTGNELALVPLEPSRSIVTIAGGPTLSNGHST--TSNFSLFPRITSEDDGRDSLSI 319

Query: 378 STRGSSCGLTGLLNLGNTCFMNSAIQCLVHTPEFARYFREDYHQEINRQNPLGMVGELAM 437
             +G   GL GL NLGNTCFMNSA+QCL HTP    YF +DY  +INR NPLGM GELA+
Sbjct: 320 LGKGEKGGLAGLSNLGNTCFMNSALQCLAHTPPIVEYFLQDYSDDINRDNPLGMCGELAI 379

Query: 438 AFGDLLRKLWAPGRTSVAPRPFKAKLARFAPQFSGYNQHDSQELLAFLLDGLHEDLNRVK 497
           AFGDLL+KLW+ GR +VAPR FK KLARFAPQFSGYNQHDSQELLAFLLDGLHEDLN+VK
Sbjct: 380 AFGDLLKKLWSSGRNAVAPRAFKTKLARFAPQFSGYNQHDSQELLAFLLDGLHEDLNKVK 439

Query: 498 HKPYIKSRDADGRPDEEVADEYWANHIARNDSIIVDVCQGQYKSTLVCPVCNKVSVTFDP 557
            KPYI+ +D+D RPD+EVA+E W  H ARNDS+IVDVCQGQYKSTLVCPVC K+S+TFDP
Sbjct: 440 RKPYIELKDSDSRPDDEVAEELWNYHKARNDSVIVDVCQGQYKSTLVCPVCGKISITFDP 499

Query: 558 FMYLSLPLQSTTTRTMTATLFTSDGSKLPSTLTVTVPKQGRCRDLIQALNSACLLKHSEK 617
           FMYLS+PL ST TR+MT T+F  DGS+LP   TV VPKQG  RDLI AL +AC L   E 
Sbjct: 500 FMYLSVPLPSTLTRSMTITVFYCDGSRLPMPYTVIVPKQGSIRDLITALGTACCLAEDES 559

Query: 618 LLLAEVRDHLIHRFLEDPLISLSTIKDDDHIAAYKIPKLTKNTK--YLQLVHRRQEQVTS 677
           LLLAEV DH I R+ E PL SLS IKDD+HI AY++ ++ K ++   L+++H  QE+   
Sbjct: 560 LLLAEVYDHKIFRYFEIPLDSLSAIKDDEHIVAYRLNQIPKGSRKAKLEILHGGQERAVL 619

Query: 678 DAHAMSGWRPFGTPLVSVVSCDDPVTKGYIQRIIKRVLSPMAKTGTLMDTDVFDSSVSHA 737
           D+   S  + FGTP V+ V+  +P++   I  +I   LSP+ K           S + + 
Sbjct: 620 DSVRGSDVKLFGTPFVTYVN-TEPLSGTDIDAVISGFLSPLHKVHA-------PSKIHNG 679

Query: 738 GEDLHPRDSSIETCTVSISSLMNKDDPKSKGAEPFKLPLQLLNKDNVCIDLSTGEESVQL 797
            ++ H  D++++      S +++  D +   A   +L  ++   D   +++   +    +
Sbjct: 680 SDNGHLADATVD----QASGILSSPDTEIDNASDRELSFRIFLTDERGLNIKPLQSESSI 739

Query: 798 PPSSSVLVYIDWSQKLMKKFDTFYLENLPEVFKSGPVMKKARTEPLSLYSCLESFLREEP 857
            P +   V ++W++   +++D+ YL +LPEV K+    KK R E +SL+SCLE+FL EEP
Sbjct: 740 SPGTVTRVLVEWNEGEHERYDSSYLSDLPEVHKTSFSAKKTRQESISLFSCLEAFLAEEP 799

Query: 858 LVPEDMWFCPQCKERRQASKKLDLWRLPEVLVIHLKRFSYSRSMKHKLETFVNFPIHDFD 917
           L P+DMWFCP CKE RQA+KKLDLW+LP++LV HLKRF+YSR +K+K++TFVNFP+HD D
Sbjct: 800 LGPDDMWFCPSCKEHRQANKKLDLWKLPDILVFHLKRFTYSRYLKNKIDTFVNFPVHDLD 859

Query: 918 LTNYVANKNNSQRQLYELYALTNHYGGMGSGHYTAHIKLIDENRWYNFDDSHISLINEEE 977
           L+ YV NK N Q  LYELYA++NHYGG+G GHYTA+ KLID+N+WY+FDDSH+S +NE E
Sbjct: 860 LSKYVKNK-NGQSYLYELYAVSNHYGGLGGGHYTAYAKLIDDNKWYHFDDSHVSSVNESE 898

Query: 978 VKSAAAYVLFYRRVRTE 991
           ++++AAYVLFYRRVR+E
Sbjct: 920 IRNSAAYVLFYRRVRSE 898

BLAST of MC03g0310 vs. ExPASy Swiss-Prot
Match: Q93Y01 (Ubiquitin carboxyl-terminal hydrolase 9 OS=Arabidopsis thaliana OX=3702 GN=UBP9 PE=2 SV=1)

HSP 1 Score: 848.6 bits (2191), Expect = 7.3e-245
Identity = 449/916 (49.02%), Postives = 608/916 (66.38%), Query Frame = 0

Query: 78  TPEEERIMIRDIALTAEANTKEGDVFYLITQRWWQHWIEYVNQDQPVNANDGSSFAEIYD 137
           TPEEE+ ++ ++   +E N KEG+++++I++RW+  W +YV Q             + Y 
Sbjct: 20  TPEEEKRIVSELITESEDNLKEGNLYFVISKRWYTSWEKYVEQS-----------TKEYI 79

Query: 138 SFGSSMLKRPANIDNSDLIYDAASEDSSVSIEIHDTLLEGRDYVLLPQEVWKQLYLWYGG 197
           S  SS   RP  IDN D+I    SE      ++   L+E  DYVL+PQEVWK+L  WY G
Sbjct: 80  SGESSEASRPGPIDNHDII---ESESDVNDPQLRRLLMERVDYVLVPQEVWKRLVEWYSG 139

Query: 198 GPTLARKVISAGLSQTELAVEVYPLRLQLLEVGKGDRSTIRISKKETIGELHRRACEIFD 257
           GP + RK+I  G      +VEVYPL L L +     R+ IR+ K+ +I EL+ + C +  
Sbjct: 140 GPPIERKLICQGFYTRSYSVEVYPLCLMLTDGRDESRTVIRLGKQASIRELYEKVCALTG 199

Query: 258 LNLEQVCIWDYYGHRKHALMNDMD-KTLDDANIQMDQDILVEIINHDNNNALGGCTSYVQ 317
           +  E+  IWDY+  RK+ L++ +  K+L+++++ MDQDIL+E+          G +S   
Sbjct: 200 VPQEKAHIWDYFDKRKNGLLDSLSYKSLEESSLHMDQDILLEV---------DGSSSSQS 259

Query: 318 DNGTTDKEATSISLESSKSNLIIAGGVSNKGASKTEVVQSQSLTSSIRESDNTYGQSGVS 377
              +T  E   + LE S+S++ IAGG +      T    + SL   I   D+      + 
Sbjct: 260 AMSSTGNELALVPLEPSRSSVTIAGGPTLSNGHST--TSNFSLFPRITSEDDGSNSLSIL 319

Query: 378 TRGSSCGLTGLLNLGNTCFMNSAIQCLVHTPEFARYFREDYHQEINRQNPLGMVGELAMA 437
            +G   GL GL NLGNTCFMNSA+QCL HTP    YF +DY  +INR NPLGM GELA+A
Sbjct: 320 GKGEKGGLAGLSNLGNTCFMNSALQCLAHTPPIVEYFLQDYSDDINRDNPLGMCGELAIA 379

Query: 438 FGDLLRKLWAPGRTSVAPRPFKAKLARFAPQFSGYNQHDSQELLAFLLDGLHEDLNRVKH 497
           FGDLL+KLW+ GR SVAPR FK KLARFAPQFSGYNQHDSQELLAFLLDGLHEDLN+VK 
Sbjct: 380 FGDLLKKLWSSGRNSVAPRAFKTKLARFAPQFSGYNQHDSQELLAFLLDGLHEDLNKVKR 439

Query: 498 KPYIKSRDADGRPDEEVADEYWANHIARNDSIIVDVCQGQYKSTLVCPVCNKVSVTFDPF 557
           KPYI+ +D+D RPD+EVA+E W  H ARNDS+IVDVCQGQYKSTLVCP C K+S+TFDPF
Sbjct: 440 KPYIELKDSDSRPDDEVAEELWNYHKARNDSVIVDVCQGQYKSTLVCPACGKISITFDPF 499

Query: 558 MYLSLPLQSTTTRTMTATLFTSDGSKLPSTLTVTVPKQGRCRDLIQALNSACLLKHSEKL 617
           MYLS+PL ST TR+MT T+F  DGS LP   TV VPK G  RDLI AL +ACLL   E L
Sbjct: 500 MYLSVPLPSTLTRSMTVTVFYCDGSHLPMPYTVIVPKNGSIRDLITALGTACLLAEDESL 559

Query: 618 LLAEVRDHLIHRFLEDPLISLSTIKDDDHIAAYKIPKLTKNT--KYLQLVHRRQEQVTSD 677
           LLAEV DH I ++ E+PL SLS+IKDD+HI AY++ ++ K +    L+++H  Q++   +
Sbjct: 560 LLAEVYDHKIFKYFENPLDSLSSIKDDEHIVAYRLNQMPKGSGKAKLEILHGGQKRPILE 619

Query: 678 AHAMSGWRPFGTPLVSVVSCDDPVTKGYIQRIIKRVLSPMAKTGTLMDTDVFDSSVSHAG 737
           +      + FGTP V+ V+  +P++   I  ++ R LSP+ K           S + +  
Sbjct: 620 SVRGRDVKLFGTPFVTYVN-TEPLSGADIDAVLSRFLSPLHKVHA-------PSKIHNGS 679

Query: 738 EDLHPRDSSIETCTVSISSLMNKDDPKSKGAEPFKLPLQLLNKDNVCIDLSTGEESVQLP 797
           E+ H  D++++  +  +SS   + D  S     F+  + L ++  +       E S+ L 
Sbjct: 680 ENGHLPDATVDEASEILSSPDTEIDDASDRELSFR--IFLTDERGLNFKPLQSESSISLG 739

Query: 798 PSSSVLVYIDWSQKLMKKFDTFYLENLPEVFKSGPVMKKARTEPLSLYSCLESFLREEPL 857
            ++ VLV  +W++   +++D+ YL +LPEV K+    KK R E +SL+SCLE+FL EEPL
Sbjct: 740 IATRVLV--EWNEGEHERYDSSYLSDLPEVHKTSFSAKKTRQESISLFSCLEAFLAEEPL 799

Query: 858 VPEDMWFCPQCKERRQASKKLDLWRLPEVLVIHLKRFSYSRSMKHKLETFVNFPIHDFDL 917
            P+DMWFCP CKE RQA+KKLDLW+LP++LV HLKRF+YSR +K+K++TFVNFP+HD DL
Sbjct: 800 GPDDMWFCPSCKEHRQANKKLDLWKLPDILVFHLKRFTYSRYLKNKIDTFVNFPVHDLDL 859

Query: 918 TNYVANKNNSQRQLYELYALTNHYGGMGSGHYTAHIKLIDENRWYNFDDSHISLINEEEV 977
           + YV NKN+ Q  LYELYA++NHYGG+G GHYTA+ KLID+N WY+FDDSH+S +NE E+
Sbjct: 860 SKYVKNKND-QSYLYELYAVSNHYGGLGGGHYTAYAKLIDDNEWYHFDDSHVSSVNESEI 897

Query: 978 KSAAAYVLFYRRVRTE 991
           K++AAYVLFYRRVR+E
Sbjct: 920 KNSAAYVLFYRRVRSE 897

BLAST of MC03g0310 vs. ExPASy Swiss-Prot
Match: Q9MAQ3 (Putative ubiquitin carboxyl-terminal hydrolase 11 OS=Arabidopsis thaliana OX=3702 GN=UBP11 PE=3 SV=2)

HSP 1 Score: 768.1 bits (1982), Expect = 1.2e-220
Identity = 431/935 (46.10%), Postives = 594/935 (63.53%), Query Frame = 0

Query: 66  VSMCSSGGRTELTPEEERIMIRDIALTAEANTKEGDVFYLITQRWWQHWIEYVNQDQPVN 125
           + +C S      TPEEER ++ ++   AEA+ KEG+++++I+ RW+  W  +V       
Sbjct: 10  IGVCES----SYTPEEERRIVTELNNEAEADLKEGNLYFVISNRWYTRWQRFV------- 69

Query: 126 ANDGSSFAEIYDSFGSSMLKRPANIDNSDLIYDAASEDSSVSIEIHDTLLEGRDYVLLPQ 185
                   E + S   S + RP  IDN D+I    SE  +   ++   L EG DY L+ Q
Sbjct: 70  ----GLLTEEFRSGEPSEVTRPGPIDNHDII---DSESDASDPQLRMMLEEGVDYTLVQQ 129

Query: 186 EVWKQLYLWYGGGPTLARKVISAGLSQTELAVEVYPLRLQLLEVGKGDRSTIRISKKETI 245
           EVW++L  WY GGP + RK+IS G      +VEVY L L L +      + IR+SK+ +I
Sbjct: 130 EVWRKLVKWYKGGPPVPRKLISQGFYTKSFSVEVYLLCLTLTDSRDESTTIIRLSKQASI 189

Query: 246 GELHRRACEIFDLNLEQVCIWDYYGHRKHALMN-DMDKTLDDANIQMDQDILVEIINHDN 305
           G+L+   C    +  E+  IWDY+  +K  L++   ++++++A +Q +QDIL+E+     
Sbjct: 190 GQLYEMVCAGKGVAKEKARIWDYFEKKKSVLLDPSSEQSVEEAGLQFNQDILLEV----- 249

Query: 306 NNALGGCTSYVQDNGTTDKEATSISLESSKS---NLIIAGGVSNKGAS---KTEVVQSQS 365
                G  S        + E   + LE  +S   +++  GG  + G S   K       +
Sbjct: 250 ----DGSASSQFVMSLAENELAMVPLEPMRSDAMDIVRGGGTLSNGHSNGFKFSFFGRNT 309

Query: 366 LTSSIRESDNTYGQSGVSTRGSSCGLTGLLNLGNTCFMNSAIQCLVHTPEFARYFREDYH 425
               +  S  T+G      +G   GL GL NLGNTCFMNS +QCL HTP    YF +DY 
Sbjct: 310 FKDDV--SSRTFG------KGEKRGLGGLQNLGNTCFMNSTLQCLAHTPPIVEYFLQDYR 369

Query: 426 QEINRQNPLGMVGELAMAFGDLLRKLWAPGRTSVAPRPFKAKLARFAPQFSGYNQHDSQE 485
            +IN +NPLGM GELA+AFG+LLRKLW+ G+ +VAPR FK KLARFAPQFSGYNQHDSQE
Sbjct: 370 SDINAKNPLGMRGELAIAFGELLRKLWSSGQNTVAPRAFKTKLARFAPQFSGYNQHDSQE 429

Query: 486 LLAFLLDGLHEDLNRVKHKPYIKSRDADGRPDEEVADEYWANHIARNDSIIVDVCQGQYK 545
           +LAFLLDGLHEDLN+VK KPYI+++D+DGRPD+EVA+E W  H ARNDS+IVDV QGQYK
Sbjct: 430 MLAFLLDGLHEDLNKVKRKPYIEAKDSDGRPDDEVAEEKWKYHKARNDSVIVDVFQGQYK 489

Query: 546 STLVCPVCNKVSVTFDPFMYLSLPLQSTTTRTMTATLFTSDGSKLPSTLTVTVPKQGRCR 605
           STLVCP C K+S+TFDPFMYLSLPL S+ TR+MT T+F  DGS LP   TVTVPK G CR
Sbjct: 490 STLVCPDCGKISITFDPFMYLSLPLPSSRTRSMTVTVFYGDGSHLPMPYTVTVPKDGSCR 549

Query: 606 DLIQALNSACLLKHSEKLLLAEVRDHLIHRFLEDPLISLSTIKDDDHIAAYKIPKLTK-- 665
           DL  AL +AC L + E LLLAEV DH + ++ E+P   L+ IKD++HI AY+  ++ K  
Sbjct: 550 DLSNALGTACCLDNDESLLLAEVYDHKVFKYYENPRELLNGIKDNEHIVAYRFKQMHKGP 609

Query: 666 NTKYLQLVHRRQEQVTSDAHAMSGWRPFGTPLVSVVSCDDPVTKGYIQRIIKRVLSPMAK 725
               L+++H  QE+ +SD     G + FGTPLV+ ++  +P++   I   I  +LSP+ +
Sbjct: 610 GKVKLEILHGEQEK-SSD----RGPKCFGTPLVTYIN-KEPLSGTDIATSISGLLSPLRR 669

Query: 726 TGTLMDTDVFDSSVSHAG-EDLHPRDSSIETCTVSISSLMNKDDPKSKGAEPFKLPLQLL 785
                   V  S V ++G E+ H  D S         S++++D          +L L LL
Sbjct: 670 --------VHMSCVVNSGNENGHVPDESSR-------SILSRDTETEDNDR--ELSLSLL 729

Query: 786 NKDNVCIDLSTGEESVQLPPSSSVLVYIDWSQKLMKKFDTFYLENLPEVFKSGPVMKKAR 845
            +D    +L   E    + P S   V + W++K  +K+D+ YL +LP+V K+  + KK  
Sbjct: 730 -RDYYSFNLQPLESDSVVNPGSVTKVLVKWNEKEHEKYDSSYLNDLPKVHKN-VLAKKTM 789

Query: 846 TEPLSLYSCLESFLREEPLVPEDMWFCPQCKERRQASKKLDLWRLPEVLVIHLKRFSYSR 905
            E +SL+SCLE+FL EEPL P+DMW+CP CKE RQA+KKLDLW+LP++LV HLKRF+YSR
Sbjct: 790 QEGISLFSCLEAFLAEEPLGPDDMWYCPGCKEHRQANKKLDLWKLPDILVFHLKRFTYSR 849

Query: 906 SMKHKLETFVNFPIHDFDLTNYVANKNNSQRQLYELYALTNHYGGMGSGHYTAHIKLIDE 965
             K+K++T VNF IHD DL+ YV N+ + Q  LYELYA++NHYGG+G GHYTA+ KL+DE
Sbjct: 850 YFKNKIDTLVNFHIHDLDLSKYVKNE-DGQSYLYELYAISNHYGGLGGGHYTAYAKLMDE 883

Query: 966 NRWYNFDDSHISLINEEEVKSAAAYVLFYRRVRTE 991
            +WYNFDDS +S +NE E+K++AAYVLFY+RV+++
Sbjct: 910 TKWYNFDDSRVSAVNESEIKTSAAYVLFYQRVKSD 883

BLAST of MC03g0310 vs. ExPASy Swiss-Prot
Match: Q9C585 (Ubiquitin carboxyl-terminal hydrolase 8 OS=Arabidopsis thaliana OX=3702 GN=UBP8 PE=2 SV=2)

HSP 1 Score: 680.6 bits (1755), Expect = 2.6e-194
Identity = 393/887 (44.31%), Postives = 540/887 (60.88%), Query Frame = 0

Query: 104 YLITQRWWQHWIEYVNQDQPVNANDGSSFAEIYDSFGSSMLKRPANIDNSDLIYDAASE- 163
           Y +  RWW+   + +   + V   +    A    S+G  M K   NI NSD+++D   E 
Sbjct: 27  YFVPLRWWKDAQDSM-PSESVEKREILYTASCGSSYGGPM-KLINNIFNSDILFDLRREG 86

Query: 164 DSSVSIEIHDTLLEGRDYVLLPQEVWKQLYLWYGGGPTLARKVISAGLSQTELAVEVYPL 223
           D+  + E  +  + GRD+ L+  ++W Q   WY       + V S      +   +VYP+
Sbjct: 87  DALQNGETGEASVSGRDFALVSSDMWLQALKWYHDDKNTEKGVKSFSAGGVDRG-DVYPV 146

Query: 224 RLQLLEVGKGDRSTIRISKKETIGELHRRACEIFDLNLEQVCIWDYYGHRKHALMNDMDK 283
           +L+L  + + +   ++I KK+   E  RRAC+IF L+ EQ+ IWD  G       +D+  
Sbjct: 147 QLRLSVLQETNSLAVKICKKDNSVECFRRACKIFSLDSEQLRIWDISGQTTLFFESDVSN 206

Query: 284 TLDDANIQMDQDILVEIINHDNNNALGGCTSYVQDNGTTDKEATSISLESSKSNLIIAGG 343
           +  D   Q DQ+IL+E+  +  ++++    S  +D  T              +N +  G 
Sbjct: 207 S-KDCQQQADQEILLELQIYGLSDSIKLKESKKEDGSTQQTNGI--------TNGMNGGT 266

Query: 344 VSNKGASKTEVVQSQSLTSSIRESDNTYGQSGVSTRGSSCGLTGLLNLGNTCFMNSAIQC 403
           V   G S                  N+    G +    + GLTGL NLGNTCFMNS++QC
Sbjct: 267 VFRFGRS------------------NSLSFLGKAGEAGTLGLTGLQNLGNTCFMNSSLQC 326

Query: 404 LVHTPEFARYFREDYHQEINRQNPLGMVGELAMAFGDLLRKLWAPGRTSVAPRPFKAKLA 463
           L HTP+   +F  +Y +EIN  NPLGM GE+A+AFGDLLR LWAPG ++VAPR FKAKLA
Sbjct: 327 LAHTPKLVDFFLGEYSKEINLDNPLGMKGEIALAFGDLLRSLWAPGASTVAPRTFKAKLA 386

Query: 464 RFAPQFSGYNQHDSQELLAFLLDGLHEDLNRVKHKPYIKSRDADGRPDEEVADEYWANHI 523
           RFAPQFSG+NQHDSQELLAFLLDGLHEDLNRVK+KPY++++D DGRPD EVADEYW NH+
Sbjct: 387 RFAPQFSGFNQHDSQELLAFLLDGLHEDLNRVKNKPYVEAKDGDGRPDAEVADEYWRNHV 446

Query: 524 ARNDSIIVDVCQGQYKSTLVCPVCNKVSVTFDPFMYLSLPLQSTTTRTMTATLFTSDGSK 583
           ARNDSIIVDVCQGQYKSTLVCP+C KVSV FDPFMYLSLPL  T+ RTM  T+ ++DGS 
Sbjct: 447 ARNDSIIVDVCQGQYKSTLVCPICKKVSVMFDPFMYLSLPLPCTSMRTMDLTVMSADGSS 506

Query: 584 LPSTLTVTVPKQGRCRDLIQALNSACLLKHSEKLLLAEVRDHLIHRFLEDPLISLSTIKD 643
           LP  LTV VPK G+  DL +AL +AC L   E LL+ EV ++ I RFLE+P  SL+ I+D
Sbjct: 507 LPIPLTVNVPKFGKFEDLHKALVTACSLPEEETLLVTEVYNNRIIRFLEEPTDSLTLIRD 566

Query: 644 DDHIAAYKIPKLTKNTKYLQLVHRRQEQVTSDAHAMSGWRPFGTPLVSVVSCDDPVTKGY 703
            D +  Y++ K   N+  +  +H++ E+      +   W+ FG PLVS + CD  V  G 
Sbjct: 567 GDKLVVYRLKKDANNSPLIVYMHQKLEEQFISGKSSPTWKAFGIPLVSRL-CD--VENGS 626

Query: 704 -IQRIIKRVLSPMAKTGTLMDTDVFDSSVSHAGEDLHPRDSSIETCTVSISSLMNKDDPK 763
            ++ +  ++LS        M T+ F  ++ +  E+    D +    T S+    + D  +
Sbjct: 627 DVENLYLKLLSSFK-----MPTEFFTENLENPTEE-EATDKTDTDGTTSVEDTNSTDVKE 686

Query: 764 SKGAEPFK-LPLQLLNKDNVCIDLSTGEESVQLPPSSSVLVYIDWSQKLMKKFDTFYLEN 823
           +  + P   L L L +     I+    +E   +  S  + V   W  K +  +DT  L +
Sbjct: 687 TTESLPDPVLRLYLTDDRGNSIEAEMLKEK-PVNKSKRLNVLARWPVKELDVYDTCLLSS 746

Query: 824 LPEVFKSGPVMKKARTEPLSLYSCLESFLREEPLVPEDMWFCPQCKERRQASKKLDLWRL 883
           LPEV KSG    K   E +SL+ CLE+FL EEPL P+DMW+CP CKE RQA KKLDLWRL
Sbjct: 747 LPEVSKSG---TKRPQESVSLFKCLEAFLTEEPLGPDDMWYCPGCKEHRQAIKKLDLWRL 806

Query: 884 PEVLVIHLKRFSYSRSMKHKLETFVNFPIHDFDLTNYVANKNNSQRQLYELYALTNHYGG 943
           PE+LVIHLKRFSYSR MK+KLE +V+FP+ + DL++Y++ KN      Y LYA++NHYG 
Sbjct: 807 PEILVIHLKRFSYSRFMKNKLEAYVDFPLDNLDLSSYISYKNGQTTYRYMLYAISNHYGS 866

Query: 944 MGSGHYTAHIKLIDENRWYNFDDSHISLINEEEVKSAAAYVLFYRRV 988
           MG GHYTA++     +RWY+FDDSH+  I++E++K++AAYVLFY+R+
Sbjct: 867 MGGGHYTAYVH-HGGDRWYDFDDSHVHQISQEKIKTSAAYVLFYKRL 869

BLAST of MC03g0310 vs. NCBI nr
Match: XP_022159318.1 (ubiquitin carboxyl-terminal hydrolase 5 [Momordica charantia])

HSP 1 Score: 1865 bits (4831), Expect = 0.0
Identity = 930/932 (99.79%), Postives = 931/932 (99.89%), Query Frame = 0

Query: 68  MCSSGGRTELTPEEERIMIRDIALTAEANTKEGDVFYLITQRWWQHWIEYVNQDQPVNAN 127
           MCSSGG TELTPEEERIMIRDIALTAEANTKEGDVFYLITQRWWQHWIEYVNQDQPVNAN
Sbjct: 1   MCSSGGSTELTPEEERIMIRDIALTAEANTKEGDVFYLITQRWWQHWIEYVNQDQPVNAN 60

Query: 128 DGSSFAEIYDSFGSSMLKRPANIDNSDLIYDAASEDSSVSIEIHDTLLEGRDYVLLPQEV 187
           DGSSFAEIYDSFGSSMLKRPANIDNSDLIYDAASEDSSVSIEIHDTLLEGRDYVLLPQEV
Sbjct: 61  DGSSFAEIYDSFGSSMLKRPANIDNSDLIYDAASEDSSVSIEIHDTLLEGRDYVLLPQEV 120

Query: 188 WKQLYLWYGGGPTLARKVISAGLSQTELAVEVYPLRLQLLEVGKGDRSTIRISKKETIGE 247
           WKQLYLWYGGGPTLARKVISAGLSQTELAVEVYPLRLQLLEVGKGDRSTIRISKKETIGE
Sbjct: 121 WKQLYLWYGGGPTLARKVISAGLSQTELAVEVYPLRLQLLEVGKGDRSTIRISKKETIGE 180

Query: 248 LHRRACEIFDLNLEQVCIWDYYGHRKHALMNDMDKTLDDANIQMDQDILVEIINHDNNNA 307
           LHRRACEIFDLNLEQVC+WDYYGHRKHALMNDMDKTLDDANIQMDQDILVEIINHDNNNA
Sbjct: 181 LHRRACEIFDLNLEQVCVWDYYGHRKHALMNDMDKTLDDANIQMDQDILVEIINHDNNNA 240

Query: 308 LGGCTSYVQDNGTTDKEATSISLESSKSNLIIAGGVSNKGASKTEVVQSQSLTSSIRESD 367
           LGGCTSYVQDNGTTDKEATSISLESSKSNLIIAGGVSNKGASKTEVVQSQSLTSSIRESD
Sbjct: 241 LGGCTSYVQDNGTTDKEATSISLESSKSNLIIAGGVSNKGASKTEVVQSQSLTSSIRESD 300

Query: 368 NTYGQSGVSTRGSSCGLTGLLNLGNTCFMNSAIQCLVHTPEFARYFREDYHQEINRQNPL 427
           NTYGQSGVSTRGSSCGLTGLLNLGNTCFMNSAIQCLVHTPEFARYFREDYHQEINRQNPL
Sbjct: 301 NTYGQSGVSTRGSSCGLTGLLNLGNTCFMNSAIQCLVHTPEFARYFREDYHQEINRQNPL 360

Query: 428 GMVGELAMAFGDLLRKLWAPGRTSVAPRPFKAKLARFAPQFSGYNQHDSQELLAFLLDGL 487
           GMVGELAMAFGDLLRKLWAPGRTSVAPRPFKAKLARFAPQFSGYNQHDSQELLAFLLDGL
Sbjct: 361 GMVGELAMAFGDLLRKLWAPGRTSVAPRPFKAKLARFAPQFSGYNQHDSQELLAFLLDGL 420

Query: 488 HEDLNRVKHKPYIKSRDADGRPDEEVADEYWANHIARNDSIIVDVCQGQYKSTLVCPVCN 547
           HEDLNRVKHKPYIKSRDADGRPDEEVADEYWANHIARNDSIIVDVCQGQYKSTLVCPVCN
Sbjct: 421 HEDLNRVKHKPYIKSRDADGRPDEEVADEYWANHIARNDSIIVDVCQGQYKSTLVCPVCN 480

Query: 548 KVSVTFDPFMYLSLPLQSTTTRTMTATLFTSDGSKLPSTLTVTVPKQGRCRDLIQALNSA 607
           KVSVTFDPFMYLSLPLQSTTTRTMTATLFTSDGSKLPSTLTVTVPKQGRCRDLIQALNSA
Sbjct: 481 KVSVTFDPFMYLSLPLQSTTTRTMTATLFTSDGSKLPSTLTVTVPKQGRCRDLIQALNSA 540

Query: 608 CLLKHSEKLLLAEVRDHLIHRFLEDPLISLSTIKDDDHIAAYKIPKLTKNTKYLQLVHRR 667
           CLLKHSEKLLLAEVRDHLIHRFLEDPLISLSTIKDDDHIAAYKIPKLTKNTKYLQLVHRR
Sbjct: 541 CLLKHSEKLLLAEVRDHLIHRFLEDPLISLSTIKDDDHIAAYKIPKLTKNTKYLQLVHRR 600

Query: 668 QEQVTSDAHAMSGWRPFGTPLVSVVSCDDPVTKGYIQRIIKRVLSPMAKTGTLMDTDVFD 727
           QEQVTSDAHAMSGWRPFGTPLVSVVSCDDPVTKGYIQRIIKRVLSPMAKTGTLMDTDVFD
Sbjct: 601 QEQVTSDAHAMSGWRPFGTPLVSVVSCDDPVTKGYIQRIIKRVLSPMAKTGTLMDTDVFD 660

Query: 728 SSVSHAGEDLHPRDSSIETCTVSISSLMNKDDPKSKGAEPFKLPLQLLNKDNVCIDLSTG 787
           SSVSHAGEDLHPRDSSIETCTVSISSLMNKDDPKSKGAEPFKLPLQLLNKDNVCIDLSTG
Sbjct: 661 SSVSHAGEDLHPRDSSIETCTVSISSLMNKDDPKSKGAEPFKLPLQLLNKDNVCIDLSTG 720

Query: 788 EESVQLPPSSSVLVYIDWSQKLMKKFDTFYLENLPEVFKSGPVMKKARTEPLSLYSCLES 847
           EESVQLPPSSSVLVYIDWSQKLMKKFDTFYLENLPEVFKSGPVMKKARTEPLSLYSCLES
Sbjct: 721 EESVQLPPSSSVLVYIDWSQKLMKKFDTFYLENLPEVFKSGPVMKKARTEPLSLYSCLES 780

Query: 848 FLREEPLVPEDMWFCPQCKERRQASKKLDLWRLPEVLVIHLKRFSYSRSMKHKLETFVNF 907
           FLREEPLVPEDMWFCPQCKERRQASKKLDLWRLPEVLVIHLKRFSYSRSMKHKLETFVNF
Sbjct: 781 FLREEPLVPEDMWFCPQCKERRQASKKLDLWRLPEVLVIHLKRFSYSRSMKHKLETFVNF 840

Query: 908 PIHDFDLTNYVANKNNSQRQLYELYALTNHYGGMGSGHYTAHIKLIDENRWYNFDDSHIS 967
           PIHDFDLTNYVANKNNSQRQLYELYALTNHYGGMGSGHYTAHIKLIDENRWYNFDDSHIS
Sbjct: 841 PIHDFDLTNYVANKNNSQRQLYELYALTNHYGGMGSGHYTAHIKLIDENRWYNFDDSHIS 900

Query: 968 LINEEEVKSAAAYVLFYRRVRTEDVSVSNGLQ 999
           LINEEEVKSAAAYVLFYRRVRTEDVSVSNGLQ
Sbjct: 901 LINEEEVKSAAAYVLFYRRVRTEDVSVSNGLQ 932

BLAST of MC03g0310 vs. NCBI nr
Match: XP_004147269.1 (ubiquitin carboxyl-terminal hydrolase 5 isoform X1 [Cucumis sativus] >XP_031741493.1 ubiquitin carboxyl-terminal hydrolase 5 isoform X1 [Cucumis sativus] >KGN64753.1 hypothetical protein Csa_014296 [Cucumis sativus])

HSP 1 Score: 1731 bits (4482), Expect = 0.0
Identity = 860/936 (91.88%), Postives = 889/936 (94.98%), Query Frame = 0

Query: 64  AEVSMCSSGGRTELTPEEERIMIRDIALTAEANTKEGDVFYLITQRWWQHWIEYVNQDQP 123
           AEVSMCSS G TELTPEEERIMIRDIAL AEANTKEGD+FYLITQRWWQHWIEYVNQDQP
Sbjct: 2   AEVSMCSSSGSTELTPEEERIMIRDIALAAEANTKEGDIFYLITQRWWQHWIEYVNQDQP 61

Query: 124 VNANDGSSFAEIYDSFGSSMLKRPANIDNSDLIYDAASEDSSVSIEIHDTLLEGRDYVLL 183
           +N NDGSSFAEIYDSFGSSMLKRPA+IDNSDLIYDAASEDSS  IEIHDTLLEGRDYVLL
Sbjct: 62  INTNDGSSFAEIYDSFGSSMLKRPASIDNSDLIYDAASEDSSAGIEIHDTLLEGRDYVLL 121

Query: 184 PQEVWKQLYLWYGGGPTLARKVISAGLSQTELAVEVYPLRLQLLEVGKGDRSTIRISKKE 243
           PQEVW QL LWYGGGP LARKVISAGLSQTEL VEVYPLRLQLLEV KGDRSTIRISKKE
Sbjct: 122 PQEVWNQLCLWYGGGPKLARKVISAGLSQTELTVEVYPLRLQLLEVPKGDRSTIRISKKE 181

Query: 244 TIGELHRRACEIFDLNLEQVCIWDYYGHRKHALMNDMDKTLDDANIQMDQDILVEIINHD 303
           TIGELHRRACEIFDLNLEQVCIWDYYGHRKHALMNDMDKTLDDANIQMDQDILVEI+NH 
Sbjct: 182 TIGELHRRACEIFDLNLEQVCIWDYYGHRKHALMNDMDKTLDDANIQMDQDILVEILNHG 241

Query: 304 NNNALGGCTSYVQDNGTTDKEATSISLESSKSNLIIAGGVSNKGASKTEVVQSQSLTSSI 363
           N+NALGGCTSYVQDNGTTDKEATSI LE+SKSNLI AGG+ NKGASKTEVVQSQ+ TSSI
Sbjct: 242 NHNALGGCTSYVQDNGTTDKEATSIHLEASKSNLISAGGMPNKGASKTEVVQSQNPTSSI 301

Query: 364 RESDNTYGQSGVSTRGSSCGLTGLLNLGNTCFMNSAIQCLVHTPEFARYFREDYHQEINR 423
           +E DN YGQSGVSTRGSSCGLTGLLNLGNTCFMNSAIQCLVHTPEFARYFREDYHQEIN 
Sbjct: 302 KELDNAYGQSGVSTRGSSCGLTGLLNLGNTCFMNSAIQCLVHTPEFARYFREDYHQEINW 361

Query: 424 QNPLGMVGELAMAFGDLLRKLWAPGRTSVAPRPFKAKLARFAPQFSGYNQHDSQELLAFL 483
           QNPLGMVGELAMAFGDLLRKLWAPGRTSVAPRPFKAKLARFAPQFSGYNQHDSQELLAFL
Sbjct: 362 QNPLGMVGELAMAFGDLLRKLWAPGRTSVAPRPFKAKLARFAPQFSGYNQHDSQELLAFL 421

Query: 484 LDGLHEDLNRVKHKPYIKSRDADGRPDEEVADEYWANHIARNDSIIVDVCQGQYKSTLVC 543
           LDGLHEDLNRVKHKPYIKSRDADGRPDEEVADEYWANHIARNDSIIVDVCQGQYKSTLVC
Sbjct: 422 LDGLHEDLNRVKHKPYIKSRDADGRPDEEVADEYWANHIARNDSIIVDVCQGQYKSTLVC 481

Query: 544 PVCNKVSVTFDPFMYLSLPLQSTTTRTMTATLFTSDGSKLPSTLTVTVPKQGRCRDLIQA 603
           PVCNKVSVTFDPFMYLSLPLQSTTTRTMT T+FTSDGSK PSTLTVTVPKQGRCRDLIQA
Sbjct: 482 PVCNKVSVTFDPFMYLSLPLQSTTTRTMTVTVFTSDGSKRPSTLTVTVPKQGRCRDLIQA 541

Query: 604 LNSACLLKHSEKLLLAEVRDHLIHRFLEDPLISLSTIKDDDHIAAYKIPKLTKNTKYLQL 663
           LN  C L+HSEKLLLAEVRDHL+HRFLEDPLISLSTIKDDDHIAAYKIPKLTKN KYLQL
Sbjct: 542 LNGTCSLRHSEKLLLAEVRDHLVHRFLEDPLISLSTIKDDDHIAAYKIPKLTKNAKYLQL 601

Query: 664 VHRRQEQVTSDAHAMSGWRPFGTPLVSVVSCDDPVTKGYIQRIIKRVLSPMAKTGTLMDT 723
           VHR QEQVTSDA  MSGWRPFGTPLVSVVSC+ PV K Y+QRI+KR LSP+AK GTLMDT
Sbjct: 602 VHRSQEQVTSDADIMSGWRPFGTPLVSVVSCEGPVKKDYLQRIVKRALSPLAKIGTLMDT 661

Query: 724 DVFDSSVSHAGEDLHPRDSSIETCTVSISSLMNKDDPKSKGAEPFKLPLQLLNKDNVCID 783
           DV DSS+SHAGEDLH  DSS ETCT S+    N DDPKSK  EPFKLPLQLLN++NVCI+
Sbjct: 662 DVSDSSMSHAGEDLHHHDSSAETCTSSL----NSDDPKSKAMEPFKLPLQLLNEENVCIE 721

Query: 784 LSTGEESVQLPPSSSVLVYIDWSQKLMKKFDTFYLENLPEVFKSGPVMKKARTEPLSLYS 843
           LS+GEE+V+LPPSSSVLVYIDWSQKL+KKFDT YLENLPEVFKSGPVMKKARTEPLSLYS
Sbjct: 722 LSSGEEAVKLPPSSSVLVYIDWSQKLLKKFDTGYLENLPEVFKSGPVMKKARTEPLSLYS 781

Query: 844 CLESFLREEPLVPEDMWFCPQCKERRQASKKLDLWRLPEVLVIHLKRFSYSRSMKHKLET 903
           CLESFLREEPLVPEDMWFCPQCKERRQASKKLDLWRLPEVLVIHLKRFSYSRSMKHKLET
Sbjct: 782 CLESFLREEPLVPEDMWFCPQCKERRQASKKLDLWRLPEVLVIHLKRFSYSRSMKHKLET 841

Query: 904 FVNFPIHDFDLTNYVANKNNSQRQLYELYALTNHYGGMGSGHYTAHIKLIDENRWYNFDD 963
           FVNFPIHDFDLTNYVANKNNSQRQLYELYALTNHYG MGSGHYTAHIKLIDENRWY+FDD
Sbjct: 842 FVNFPIHDFDLTNYVANKNNSQRQLYELYALTNHYGSMGSGHYTAHIKLIDENRWYSFDD 901

Query: 964 SHISLINEEEVKSAAAYVLFYRRVRTEDVSVSNGLQ 999
           SHISLINEEEVKSAAAYVLFYRRV+TEDVS+SNG+Q
Sbjct: 902 SHISLINEEEVKSAAAYVLFYRRVKTEDVSLSNGVQ 933

BLAST of MC03g0310 vs. NCBI nr
Match: XP_038894344.1 (ubiquitin carboxyl-terminal hydrolase 5 isoform X2 [Benincasa hispida])

HSP 1 Score: 1727 bits (4474), Expect = 0.0
Identity = 861/936 (91.99%), Postives = 885/936 (94.55%), Query Frame = 0

Query: 64  AEVSMCSSGGRTELTPEEERIMIRDIALTAEANTKEGDVFYLITQRWWQHWIEYVNQDQP 123
           AEVSMCSS G TELTPEEERIMIRDIAL AEANTKEGD+FYLITQRWWQHWIEYVNQDQP
Sbjct: 2   AEVSMCSSSGCTELTPEEERIMIRDIALAAEANTKEGDIFYLITQRWWQHWIEYVNQDQP 61

Query: 124 VNANDGSSFAEIYDSFGSSMLKRPANIDNSDLIYDAASEDSSVSIEIHDTLLEGRDYVLL 183
           +NANDGSSFAEIYDSFGSSMLKRPANIDNSDLIYDA SEDSS SIEIHDTLLEGRDYVLL
Sbjct: 62  INANDGSSFAEIYDSFGSSMLKRPANIDNSDLIYDAVSEDSSASIEIHDTLLEGRDYVLL 121

Query: 184 PQEVWKQLYLWYGGGPTLARKVISAGLSQTELAVEVYPLRLQLLEVGKGDRSTIRISKKE 243
           PQEVW QLYLWYGGGP LARKVISAGLSQTELAVEVYPLRLQLLEV KGDRSTIRISKKE
Sbjct: 122 PQEVWNQLYLWYGGGPKLARKVISAGLSQTELAVEVYPLRLQLLEVPKGDRSTIRISKKE 181

Query: 244 TIGELHRRACEIFDLNLEQVCIWDYYGHRKHALMNDMDKTLDDANIQMDQDILVEIINHD 303
           TIGELHRRACEIFDLNLEQVCIWDYYGHRKHALMNDMDKTLDDANIQMDQDILVEIINH 
Sbjct: 182 TIGELHRRACEIFDLNLEQVCIWDYYGHRKHALMNDMDKTLDDANIQMDQDILVEIINHG 241

Query: 304 NNNALGGCTSYVQDNGTTDKEATSISLESSKSNLIIAGGVSNKGASKTEVVQSQSLTSSI 363
           N+NALGGC SYVQDNGTTDKEATS  LE SKSNLI AGG+SNKGASKTEVVQSQ+ TSSI
Sbjct: 242 NHNALGGCASYVQDNGTTDKEATSTHLEPSKSNLISAGGMSNKGASKTEVVQSQNPTSSI 301

Query: 364 RESDNTYGQSGVSTRGSSCGLTGLLNLGNTCFMNSAIQCLVHTPEFARYFREDYHQEINR 423
           +E DNTYGQSGVSTRGSSCGLTGLLNLGNTCFMNSAIQCLVHTPEFARYFREDYHQEIN 
Sbjct: 302 KELDNTYGQSGVSTRGSSCGLTGLLNLGNTCFMNSAIQCLVHTPEFARYFREDYHQEINW 361

Query: 424 QNPLGMVGELAMAFGDLLRKLWAPGRTSVAPRPFKAKLARFAPQFSGYNQHDSQELLAFL 483
           QNPLGMVGELAMAFGDLLRKLWAPGRTSVAPRPFKAKLARFAPQFSGYNQHDSQELLAFL
Sbjct: 362 QNPLGMVGELAMAFGDLLRKLWAPGRTSVAPRPFKAKLARFAPQFSGYNQHDSQELLAFL 421

Query: 484 LDGLHEDLNRVKHKPYIKSRDADGRPDEEVADEYWANHIARNDSIIVDVCQGQYKSTLVC 543
           LDGLHEDLNRVKHKPYIKSRDADGRPDEEVADEYWANHIARNDSIIVDVCQGQYKSTLVC
Sbjct: 422 LDGLHEDLNRVKHKPYIKSRDADGRPDEEVADEYWANHIARNDSIIVDVCQGQYKSTLVC 481

Query: 544 PVCNKVSVTFDPFMYLSLPLQSTTTRTMTATLFTSDGSKLPSTLTVTVPKQGRCRDLIQA 603
           PVCNKVSVTFDPFMYLSLPLQSTTTRTMT T+FTSDGSK PSTLTVTVPKQGRCRDLIQA
Sbjct: 482 PVCNKVSVTFDPFMYLSLPLQSTTTRTMTVTVFTSDGSKRPSTLTVTVPKQGRCRDLIQA 541

Query: 604 LNSACLLKHSEKLLLAEVRDHLIHRFLEDPLISLSTIKDDDHIAAYKIPKLTKNTKYLQL 663
           LN  C L+HSEKLLLAEVRDHLI RFLEDPLI LSTI+DDDHIAAYKIPKLTKNTKYLQL
Sbjct: 542 LNGTCSLRHSEKLLLAEVRDHLIQRFLEDPLILLSTIRDDDHIAAYKIPKLTKNTKYLQL 601

Query: 664 VHRRQEQVTSDAHAMSGWRPFGTPLVSVVSCDDPVTKGYIQRIIKRVLSPMAKTGTLMDT 723
           VHR QEQV SDAH MSGWRPFGTPLVSVVSCD PV K Y+QRI+KR LSPMAK GTLMDT
Sbjct: 602 VHRSQEQVNSDAHKMSGWRPFGTPLVSVVSCDGPVKKDYLQRIVKRALSPMAKAGTLMDT 661

Query: 724 DVFDSSVSHAGEDLHPRDSSIETCTVSISSLMNKDDPKSKGAEPFKLPLQLLNKDNVCID 783
           DV DSS+SH GED H  D   ETCT S    MN DDPKSK  EPFKLPLQLLN++NVCI+
Sbjct: 662 DVSDSSMSHIGEDHHHHDFYAETCTSS----MNSDDPKSKATEPFKLPLQLLNEENVCIE 721

Query: 784 LSTGEESVQLPPSSSVLVYIDWSQKLMKKFDTFYLENLPEVFKSGPVMKKARTEPLSLYS 843
           LS+GEE+++LPPSSSVLVYIDWSQKL+KKFDT YLENLPEVFKSGPVMKK RTEPLSLYS
Sbjct: 722 LSSGEEALKLPPSSSVLVYIDWSQKLLKKFDTGYLENLPEVFKSGPVMKKPRTEPLSLYS 781

Query: 844 CLESFLREEPLVPEDMWFCPQCKERRQASKKLDLWRLPEVLVIHLKRFSYSRSMKHKLET 903
           CLESFLREEPLVPEDMWFCPQCKERRQASKKLDLWRLPEVLVIHLKRFSYSRSMKHKLET
Sbjct: 782 CLESFLREEPLVPEDMWFCPQCKERRQASKKLDLWRLPEVLVIHLKRFSYSRSMKHKLET 841

Query: 904 FVNFPIHDFDLTNYVANKNNSQRQLYELYALTNHYGGMGSGHYTAHIKLIDENRWYNFDD 963
           FVNFPIHDFDLTNYVANKNNSQRQLYELYALTNHYG MGSGHYTAHIKLIDENRWY+FDD
Sbjct: 842 FVNFPIHDFDLTNYVANKNNSQRQLYELYALTNHYGSMGSGHYTAHIKLIDENRWYSFDD 901

Query: 964 SHISLINEEEVKSAAAYVLFYRRVRTEDVSVSNGLQ 999
           SHISLINEEEV+SAAAYVLFYRRV+TEDVSVSNG+Q
Sbjct: 902 SHISLINEEEVRSAAAYVLFYRRVKTEDVSVSNGVQ 933

BLAST of MC03g0310 vs. NCBI nr
Match: XP_038894343.1 (ubiquitin carboxyl-terminal hydrolase 5 isoform X1 [Benincasa hispida])

HSP 1 Score: 1727 bits (4474), Expect = 0.0
Identity = 861/936 (91.99%), Postives = 885/936 (94.55%), Query Frame = 0

Query: 64  AEVSMCSSGGRTELTPEEERIMIRDIALTAEANTKEGDVFYLITQRWWQHWIEYVNQDQP 123
           AEVSMCSS G TELTPEEERIMIRDIAL AEANTKEGD+FYLITQRWWQHWIEYVNQDQP
Sbjct: 48  AEVSMCSSSGCTELTPEEERIMIRDIALAAEANTKEGDIFYLITQRWWQHWIEYVNQDQP 107

Query: 124 VNANDGSSFAEIYDSFGSSMLKRPANIDNSDLIYDAASEDSSVSIEIHDTLLEGRDYVLL 183
           +NANDGSSFAEIYDSFGSSMLKRPANIDNSDLIYDA SEDSS SIEIHDTLLEGRDYVLL
Sbjct: 108 INANDGSSFAEIYDSFGSSMLKRPANIDNSDLIYDAVSEDSSASIEIHDTLLEGRDYVLL 167

Query: 184 PQEVWKQLYLWYGGGPTLARKVISAGLSQTELAVEVYPLRLQLLEVGKGDRSTIRISKKE 243
           PQEVW QLYLWYGGGP LARKVISAGLSQTELAVEVYPLRLQLLEV KGDRSTIRISKKE
Sbjct: 168 PQEVWNQLYLWYGGGPKLARKVISAGLSQTELAVEVYPLRLQLLEVPKGDRSTIRISKKE 227

Query: 244 TIGELHRRACEIFDLNLEQVCIWDYYGHRKHALMNDMDKTLDDANIQMDQDILVEIINHD 303
           TIGELHRRACEIFDLNLEQVCIWDYYGHRKHALMNDMDKTLDDANIQMDQDILVEIINH 
Sbjct: 228 TIGELHRRACEIFDLNLEQVCIWDYYGHRKHALMNDMDKTLDDANIQMDQDILVEIINHG 287

Query: 304 NNNALGGCTSYVQDNGTTDKEATSISLESSKSNLIIAGGVSNKGASKTEVVQSQSLTSSI 363
           N+NALGGC SYVQDNGTTDKEATS  LE SKSNLI AGG+SNKGASKTEVVQSQ+ TSSI
Sbjct: 288 NHNALGGCASYVQDNGTTDKEATSTHLEPSKSNLISAGGMSNKGASKTEVVQSQNPTSSI 347

Query: 364 RESDNTYGQSGVSTRGSSCGLTGLLNLGNTCFMNSAIQCLVHTPEFARYFREDYHQEINR 423
           +E DNTYGQSGVSTRGSSCGLTGLLNLGNTCFMNSAIQCLVHTPEFARYFREDYHQEIN 
Sbjct: 348 KELDNTYGQSGVSTRGSSCGLTGLLNLGNTCFMNSAIQCLVHTPEFARYFREDYHQEINW 407

Query: 424 QNPLGMVGELAMAFGDLLRKLWAPGRTSVAPRPFKAKLARFAPQFSGYNQHDSQELLAFL 483
           QNPLGMVGELAMAFGDLLRKLWAPGRTSVAPRPFKAKLARFAPQFSGYNQHDSQELLAFL
Sbjct: 408 QNPLGMVGELAMAFGDLLRKLWAPGRTSVAPRPFKAKLARFAPQFSGYNQHDSQELLAFL 467

Query: 484 LDGLHEDLNRVKHKPYIKSRDADGRPDEEVADEYWANHIARNDSIIVDVCQGQYKSTLVC 543
           LDGLHEDLNRVKHKPYIKSRDADGRPDEEVADEYWANHIARNDSIIVDVCQGQYKSTLVC
Sbjct: 468 LDGLHEDLNRVKHKPYIKSRDADGRPDEEVADEYWANHIARNDSIIVDVCQGQYKSTLVC 527

Query: 544 PVCNKVSVTFDPFMYLSLPLQSTTTRTMTATLFTSDGSKLPSTLTVTVPKQGRCRDLIQA 603
           PVCNKVSVTFDPFMYLSLPLQSTTTRTMT T+FTSDGSK PSTLTVTVPKQGRCRDLIQA
Sbjct: 528 PVCNKVSVTFDPFMYLSLPLQSTTTRTMTVTVFTSDGSKRPSTLTVTVPKQGRCRDLIQA 587

Query: 604 LNSACLLKHSEKLLLAEVRDHLIHRFLEDPLISLSTIKDDDHIAAYKIPKLTKNTKYLQL 663
           LN  C L+HSEKLLLAEVRDHLI RFLEDPLI LSTI+DDDHIAAYKIPKLTKNTKYLQL
Sbjct: 588 LNGTCSLRHSEKLLLAEVRDHLIQRFLEDPLILLSTIRDDDHIAAYKIPKLTKNTKYLQL 647

Query: 664 VHRRQEQVTSDAHAMSGWRPFGTPLVSVVSCDDPVTKGYIQRIIKRVLSPMAKTGTLMDT 723
           VHR QEQV SDAH MSGWRPFGTPLVSVVSCD PV K Y+QRI+KR LSPMAK GTLMDT
Sbjct: 648 VHRSQEQVNSDAHKMSGWRPFGTPLVSVVSCDGPVKKDYLQRIVKRALSPMAKAGTLMDT 707

Query: 724 DVFDSSVSHAGEDLHPRDSSIETCTVSISSLMNKDDPKSKGAEPFKLPLQLLNKDNVCID 783
           DV DSS+SH GED H  D   ETCT S    MN DDPKSK  EPFKLPLQLLN++NVCI+
Sbjct: 708 DVSDSSMSHIGEDHHHHDFYAETCTSS----MNSDDPKSKATEPFKLPLQLLNEENVCIE 767

Query: 784 LSTGEESVQLPPSSSVLVYIDWSQKLMKKFDTFYLENLPEVFKSGPVMKKARTEPLSLYS 843
           LS+GEE+++LPPSSSVLVYIDWSQKL+KKFDT YLENLPEVFKSGPVMKK RTEPLSLYS
Sbjct: 768 LSSGEEALKLPPSSSVLVYIDWSQKLLKKFDTGYLENLPEVFKSGPVMKKPRTEPLSLYS 827

Query: 844 CLESFLREEPLVPEDMWFCPQCKERRQASKKLDLWRLPEVLVIHLKRFSYSRSMKHKLET 903
           CLESFLREEPLVPEDMWFCPQCKERRQASKKLDLWRLPEVLVIHLKRFSYSRSMKHKLET
Sbjct: 828 CLESFLREEPLVPEDMWFCPQCKERRQASKKLDLWRLPEVLVIHLKRFSYSRSMKHKLET 887

Query: 904 FVNFPIHDFDLTNYVANKNNSQRQLYELYALTNHYGGMGSGHYTAHIKLIDENRWYNFDD 963
           FVNFPIHDFDLTNYVANKNNSQRQLYELYALTNHYG MGSGHYTAHIKLIDENRWY+FDD
Sbjct: 888 FVNFPIHDFDLTNYVANKNNSQRQLYELYALTNHYGSMGSGHYTAHIKLIDENRWYSFDD 947

Query: 964 SHISLINEEEVKSAAAYVLFYRRVRTEDVSVSNGLQ 999
           SHISLINEEEV+SAAAYVLFYRRV+TEDVSVSNG+Q
Sbjct: 948 SHISLINEEEVRSAAAYVLFYRRVKTEDVSVSNGVQ 979

BLAST of MC03g0310 vs. NCBI nr
Match: XP_008463584.1 (PREDICTED: ubiquitin carboxyl-terminal hydrolase 5 isoform X1 [Cucumis melo])

HSP 1 Score: 1721 bits (4457), Expect = 0.0
Identity = 858/937 (91.57%), Postives = 889/937 (94.88%), Query Frame = 0

Query: 64  AEVSMCSSGGRTELTPEEERIMIRDIALTAEANTKEGDVFYLITQRWWQHWIEYVNQDQP 123
           AEVSMCSS G TELTPEEERIMIRDIAL AEANTKEGD+FYLITQRWWQHWIEYVNQDQP
Sbjct: 2   AEVSMCSSSGSTELTPEEERIMIRDIALAAEANTKEGDIFYLITQRWWQHWIEYVNQDQP 61

Query: 124 VNANDGSSFAEIYDSFGSSMLKRPANIDNSDLIYDAASEDSSVSIEIHDTLLEGRDYVLL 183
           +NANDGSSFAEIYDSFGSSMLKRPA+IDNSDLIYDAASEDSS  IEIHDTLLEGRDYVLL
Sbjct: 62  INANDGSSFAEIYDSFGSSMLKRPASIDNSDLIYDAASEDSSAGIEIHDTLLEGRDYVLL 121

Query: 184 PQEVWKQLYLWYGGGPTLARKVISAGLSQTELAVEVYPLRLQLLEVGKGDRSTIRISKKE 243
           PQEVW QL LWYGGGP LARKVISAGLSQTEL VEVYPLRLQLLEV KGDRSTIRISKKE
Sbjct: 122 PQEVWNQLCLWYGGGPKLARKVISAGLSQTELTVEVYPLRLQLLEVPKGDRSTIRISKKE 181

Query: 244 TIGELHRRACEIFDLNLEQVCIWDYYGHRKHALMNDMDKTLDDANIQMDQDILVEIINHD 303
           TIGELHRRACEIFDLNLEQVCIWDYYGHRKHALMNDMDKTLDDANIQMDQDILVEI+NH 
Sbjct: 182 TIGELHRRACEIFDLNLEQVCIWDYYGHRKHALMNDMDKTLDDANIQMDQDILVEILNHG 241

Query: 304 NNNALGGCTSYVQDNGTTDKEATSISLESSKSNLIIAGGV-SNKGASKTEVVQSQSLTSS 363
           N+NALGGC SYVQDNGTTDKEATSI LE+SKSNLI AGG+ +NKGASKTEVVQSQ+ TSS
Sbjct: 242 NHNALGGCASYVQDNGTTDKEATSIYLEASKSNLISAGGMPNNKGASKTEVVQSQNPTSS 301

Query: 364 IRESDNTYGQSGVSTRGSSCGLTGLLNLGNTCFMNSAIQCLVHTPEFARYFREDYHQEIN 423
           I+E DNTYGQSGVSTRGSSCGLTGLLNLGNTCFMNSAIQCLVHTPEFARYFREDYHQEIN
Sbjct: 302 IKELDNTYGQSGVSTRGSSCGLTGLLNLGNTCFMNSAIQCLVHTPEFARYFREDYHQEIN 361

Query: 424 RQNPLGMVGELAMAFGDLLRKLWAPGRTSVAPRPFKAKLARFAPQFSGYNQHDSQELLAF 483
            QNPLGMVGELAMAFGDLLRKLWAPGRTSVAPRPFKAKLARFAPQFSGYNQHDSQELLAF
Sbjct: 362 WQNPLGMVGELAMAFGDLLRKLWAPGRTSVAPRPFKAKLARFAPQFSGYNQHDSQELLAF 421

Query: 484 LLDGLHEDLNRVKHKPYIKSRDADGRPDEEVADEYWANHIARNDSIIVDVCQGQYKSTLV 543
           LLDGLHEDLNRVKHKPYIKSRDADGRPDEEVADEYWANHIARNDSIIVDVCQGQYKSTLV
Sbjct: 422 LLDGLHEDLNRVKHKPYIKSRDADGRPDEEVADEYWANHIARNDSIIVDVCQGQYKSTLV 481

Query: 544 CPVCNKVSVTFDPFMYLSLPLQSTTTRTMTATLFTSDGSKLPSTLTVTVPKQGRCRDLIQ 603
           CPVCNKVSVTFDPFMYLSLPLQSTTTRTMT T+FTSDGSK PST TVTVPKQGRCRDLIQ
Sbjct: 482 CPVCNKVSVTFDPFMYLSLPLQSTTTRTMTVTVFTSDGSKRPSTFTVTVPKQGRCRDLIQ 541

Query: 604 ALNSACLLKHSEKLLLAEVRDHLIHRFLEDPLISLSTIKDDDHIAAYKIPKLTKNTKYLQ 663
           ALN  C L+HSEKLLLAEVRDHLIHRFLEDPLISLSTIKDDDHIAAYKIPKLTKN KYLQ
Sbjct: 542 ALNGTCSLRHSEKLLLAEVRDHLIHRFLEDPLISLSTIKDDDHIAAYKIPKLTKNAKYLQ 601

Query: 664 LVHRRQEQVTSDAHAMSGWRPFGTPLVSVVSCDDPVTKGYIQRIIKRVLSPMAKTGTLMD 723
           LVHR QEQVTSDAH MSGWRPFGTPLVSVVSC+ PV K  +QRI+KR LSP+AK GTLMD
Sbjct: 602 LVHRSQEQVTSDAHIMSGWRPFGTPLVSVVSCEGPVKKDCLQRIVKRALSPLAKIGTLMD 661

Query: 724 TDVFDSSVSHAGEDLHPRDSSIETCTVSISSLMNKDDPKSKGAEPFKLPLQLLNKDNVCI 783
            DV DSS+SHAGEDL+  DSS ETCT S+    N DDPKSK  EPFKLPLQLLN++NVCI
Sbjct: 662 NDVSDSSMSHAGEDLNHHDSSAETCTSSL----NSDDPKSKAMEPFKLPLQLLNEENVCI 721

Query: 784 DLSTGEESVQLPPSSSVLVYIDWSQKLMKKFDTFYLENLPEVFKSGPVMKKARTEPLSLY 843
           +LS+GEE+V+LPPS+SVLVYIDWSQKL+KKFDT YLENLPEVFKSGPVMKKARTEPLSLY
Sbjct: 722 ELSSGEEAVRLPPSTSVLVYIDWSQKLLKKFDTGYLENLPEVFKSGPVMKKARTEPLSLY 781

Query: 844 SCLESFLREEPLVPEDMWFCPQCKERRQASKKLDLWRLPEVLVIHLKRFSYSRSMKHKLE 903
           SCLESFLREEPLVPEDMWFCPQCKERRQASKKLDLWRLPEVLVIHLKRFSYSRSMKHKLE
Sbjct: 782 SCLESFLREEPLVPEDMWFCPQCKERRQASKKLDLWRLPEVLVIHLKRFSYSRSMKHKLE 841

Query: 904 TFVNFPIHDFDLTNYVANKNNSQRQLYELYALTNHYGGMGSGHYTAHIKLIDENRWYNFD 963
           TFVNFPIHDFDLTNYVANKNNSQRQLYELYALTNHYG MGSGHYTAHIKLIDENRWY+FD
Sbjct: 842 TFVNFPIHDFDLTNYVANKNNSQRQLYELYALTNHYGSMGSGHYTAHIKLIDENRWYSFD 901

Query: 964 DSHISLINEEEVKSAAAYVLFYRRVRTEDVSVSNGLQ 999
           DSHISLINEEEVKSAAAYVLFYRRV+TEDVS+SNG+Q
Sbjct: 902 DSHISLINEEEVKSAAAYVLFYRRVKTEDVSLSNGVQ 934

BLAST of MC03g0310 vs. ExPASy TrEMBL
Match: A0A6J1DYG8 (Ubiquitin carboxyl-terminal hydrolase OS=Momordica charantia OX=3673 GN=LOC111025728 PE=3 SV=1)

HSP 1 Score: 1865 bits (4831), Expect = 0.0
Identity = 930/932 (99.79%), Postives = 931/932 (99.89%), Query Frame = 0

Query: 68  MCSSGGRTELTPEEERIMIRDIALTAEANTKEGDVFYLITQRWWQHWIEYVNQDQPVNAN 127
           MCSSGG TELTPEEERIMIRDIALTAEANTKEGDVFYLITQRWWQHWIEYVNQDQPVNAN
Sbjct: 1   MCSSGGSTELTPEEERIMIRDIALTAEANTKEGDVFYLITQRWWQHWIEYVNQDQPVNAN 60

Query: 128 DGSSFAEIYDSFGSSMLKRPANIDNSDLIYDAASEDSSVSIEIHDTLLEGRDYVLLPQEV 187
           DGSSFAEIYDSFGSSMLKRPANIDNSDLIYDAASEDSSVSIEIHDTLLEGRDYVLLPQEV
Sbjct: 61  DGSSFAEIYDSFGSSMLKRPANIDNSDLIYDAASEDSSVSIEIHDTLLEGRDYVLLPQEV 120

Query: 188 WKQLYLWYGGGPTLARKVISAGLSQTELAVEVYPLRLQLLEVGKGDRSTIRISKKETIGE 247
           WKQLYLWYGGGPTLARKVISAGLSQTELAVEVYPLRLQLLEVGKGDRSTIRISKKETIGE
Sbjct: 121 WKQLYLWYGGGPTLARKVISAGLSQTELAVEVYPLRLQLLEVGKGDRSTIRISKKETIGE 180

Query: 248 LHRRACEIFDLNLEQVCIWDYYGHRKHALMNDMDKTLDDANIQMDQDILVEIINHDNNNA 307
           LHRRACEIFDLNLEQVC+WDYYGHRKHALMNDMDKTLDDANIQMDQDILVEIINHDNNNA
Sbjct: 181 LHRRACEIFDLNLEQVCVWDYYGHRKHALMNDMDKTLDDANIQMDQDILVEIINHDNNNA 240

Query: 308 LGGCTSYVQDNGTTDKEATSISLESSKSNLIIAGGVSNKGASKTEVVQSQSLTSSIRESD 367
           LGGCTSYVQDNGTTDKEATSISLESSKSNLIIAGGVSNKGASKTEVVQSQSLTSSIRESD
Sbjct: 241 LGGCTSYVQDNGTTDKEATSISLESSKSNLIIAGGVSNKGASKTEVVQSQSLTSSIRESD 300

Query: 368 NTYGQSGVSTRGSSCGLTGLLNLGNTCFMNSAIQCLVHTPEFARYFREDYHQEINRQNPL 427
           NTYGQSGVSTRGSSCGLTGLLNLGNTCFMNSAIQCLVHTPEFARYFREDYHQEINRQNPL
Sbjct: 301 NTYGQSGVSTRGSSCGLTGLLNLGNTCFMNSAIQCLVHTPEFARYFREDYHQEINRQNPL 360

Query: 428 GMVGELAMAFGDLLRKLWAPGRTSVAPRPFKAKLARFAPQFSGYNQHDSQELLAFLLDGL 487
           GMVGELAMAFGDLLRKLWAPGRTSVAPRPFKAKLARFAPQFSGYNQHDSQELLAFLLDGL
Sbjct: 361 GMVGELAMAFGDLLRKLWAPGRTSVAPRPFKAKLARFAPQFSGYNQHDSQELLAFLLDGL 420

Query: 488 HEDLNRVKHKPYIKSRDADGRPDEEVADEYWANHIARNDSIIVDVCQGQYKSTLVCPVCN 547
           HEDLNRVKHKPYIKSRDADGRPDEEVADEYWANHIARNDSIIVDVCQGQYKSTLVCPVCN
Sbjct: 421 HEDLNRVKHKPYIKSRDADGRPDEEVADEYWANHIARNDSIIVDVCQGQYKSTLVCPVCN 480

Query: 548 KVSVTFDPFMYLSLPLQSTTTRTMTATLFTSDGSKLPSTLTVTVPKQGRCRDLIQALNSA 607
           KVSVTFDPFMYLSLPLQSTTTRTMTATLFTSDGSKLPSTLTVTVPKQGRCRDLIQALNSA
Sbjct: 481 KVSVTFDPFMYLSLPLQSTTTRTMTATLFTSDGSKLPSTLTVTVPKQGRCRDLIQALNSA 540

Query: 608 CLLKHSEKLLLAEVRDHLIHRFLEDPLISLSTIKDDDHIAAYKIPKLTKNTKYLQLVHRR 667
           CLLKHSEKLLLAEVRDHLIHRFLEDPLISLSTIKDDDHIAAYKIPKLTKNTKYLQLVHRR
Sbjct: 541 CLLKHSEKLLLAEVRDHLIHRFLEDPLISLSTIKDDDHIAAYKIPKLTKNTKYLQLVHRR 600

Query: 668 QEQVTSDAHAMSGWRPFGTPLVSVVSCDDPVTKGYIQRIIKRVLSPMAKTGTLMDTDVFD 727
           QEQVTSDAHAMSGWRPFGTPLVSVVSCDDPVTKGYIQRIIKRVLSPMAKTGTLMDTDVFD
Sbjct: 601 QEQVTSDAHAMSGWRPFGTPLVSVVSCDDPVTKGYIQRIIKRVLSPMAKTGTLMDTDVFD 660

Query: 728 SSVSHAGEDLHPRDSSIETCTVSISSLMNKDDPKSKGAEPFKLPLQLLNKDNVCIDLSTG 787
           SSVSHAGEDLHPRDSSIETCTVSISSLMNKDDPKSKGAEPFKLPLQLLNKDNVCIDLSTG
Sbjct: 661 SSVSHAGEDLHPRDSSIETCTVSISSLMNKDDPKSKGAEPFKLPLQLLNKDNVCIDLSTG 720

Query: 788 EESVQLPPSSSVLVYIDWSQKLMKKFDTFYLENLPEVFKSGPVMKKARTEPLSLYSCLES 847
           EESVQLPPSSSVLVYIDWSQKLMKKFDTFYLENLPEVFKSGPVMKKARTEPLSLYSCLES
Sbjct: 721 EESVQLPPSSSVLVYIDWSQKLMKKFDTFYLENLPEVFKSGPVMKKARTEPLSLYSCLES 780

Query: 848 FLREEPLVPEDMWFCPQCKERRQASKKLDLWRLPEVLVIHLKRFSYSRSMKHKLETFVNF 907
           FLREEPLVPEDMWFCPQCKERRQASKKLDLWRLPEVLVIHLKRFSYSRSMKHKLETFVNF
Sbjct: 781 FLREEPLVPEDMWFCPQCKERRQASKKLDLWRLPEVLVIHLKRFSYSRSMKHKLETFVNF 840

Query: 908 PIHDFDLTNYVANKNNSQRQLYELYALTNHYGGMGSGHYTAHIKLIDENRWYNFDDSHIS 967
           PIHDFDLTNYVANKNNSQRQLYELYALTNHYGGMGSGHYTAHIKLIDENRWYNFDDSHIS
Sbjct: 841 PIHDFDLTNYVANKNNSQRQLYELYALTNHYGGMGSGHYTAHIKLIDENRWYNFDDSHIS 900

Query: 968 LINEEEVKSAAAYVLFYRRVRTEDVSVSNGLQ 999
           LINEEEVKSAAAYVLFYRRVRTEDVSVSNGLQ
Sbjct: 901 LINEEEVKSAAAYVLFYRRVRTEDVSVSNGLQ 932

BLAST of MC03g0310 vs. ExPASy TrEMBL
Match: A0A0A0LSJ1 (Ubiquitin carboxyl-terminal hydrolase OS=Cucumis sativus OX=3659 GN=Csa_1G087940 PE=3 SV=1)

HSP 1 Score: 1731 bits (4482), Expect = 0.0
Identity = 860/936 (91.88%), Postives = 889/936 (94.98%), Query Frame = 0

Query: 64  AEVSMCSSGGRTELTPEEERIMIRDIALTAEANTKEGDVFYLITQRWWQHWIEYVNQDQP 123
           AEVSMCSS G TELTPEEERIMIRDIAL AEANTKEGD+FYLITQRWWQHWIEYVNQDQP
Sbjct: 2   AEVSMCSSSGSTELTPEEERIMIRDIALAAEANTKEGDIFYLITQRWWQHWIEYVNQDQP 61

Query: 124 VNANDGSSFAEIYDSFGSSMLKRPANIDNSDLIYDAASEDSSVSIEIHDTLLEGRDYVLL 183
           +N NDGSSFAEIYDSFGSSMLKRPA+IDNSDLIYDAASEDSS  IEIHDTLLEGRDYVLL
Sbjct: 62  INTNDGSSFAEIYDSFGSSMLKRPASIDNSDLIYDAASEDSSAGIEIHDTLLEGRDYVLL 121

Query: 184 PQEVWKQLYLWYGGGPTLARKVISAGLSQTELAVEVYPLRLQLLEVGKGDRSTIRISKKE 243
           PQEVW QL LWYGGGP LARKVISAGLSQTEL VEVYPLRLQLLEV KGDRSTIRISKKE
Sbjct: 122 PQEVWNQLCLWYGGGPKLARKVISAGLSQTELTVEVYPLRLQLLEVPKGDRSTIRISKKE 181

Query: 244 TIGELHRRACEIFDLNLEQVCIWDYYGHRKHALMNDMDKTLDDANIQMDQDILVEIINHD 303
           TIGELHRRACEIFDLNLEQVCIWDYYGHRKHALMNDMDKTLDDANIQMDQDILVEI+NH 
Sbjct: 182 TIGELHRRACEIFDLNLEQVCIWDYYGHRKHALMNDMDKTLDDANIQMDQDILVEILNHG 241

Query: 304 NNNALGGCTSYVQDNGTTDKEATSISLESSKSNLIIAGGVSNKGASKTEVVQSQSLTSSI 363
           N+NALGGCTSYVQDNGTTDKEATSI LE+SKSNLI AGG+ NKGASKTEVVQSQ+ TSSI
Sbjct: 242 NHNALGGCTSYVQDNGTTDKEATSIHLEASKSNLISAGGMPNKGASKTEVVQSQNPTSSI 301

Query: 364 RESDNTYGQSGVSTRGSSCGLTGLLNLGNTCFMNSAIQCLVHTPEFARYFREDYHQEINR 423
           +E DN YGQSGVSTRGSSCGLTGLLNLGNTCFMNSAIQCLVHTPEFARYFREDYHQEIN 
Sbjct: 302 KELDNAYGQSGVSTRGSSCGLTGLLNLGNTCFMNSAIQCLVHTPEFARYFREDYHQEINW 361

Query: 424 QNPLGMVGELAMAFGDLLRKLWAPGRTSVAPRPFKAKLARFAPQFSGYNQHDSQELLAFL 483
           QNPLGMVGELAMAFGDLLRKLWAPGRTSVAPRPFKAKLARFAPQFSGYNQHDSQELLAFL
Sbjct: 362 QNPLGMVGELAMAFGDLLRKLWAPGRTSVAPRPFKAKLARFAPQFSGYNQHDSQELLAFL 421

Query: 484 LDGLHEDLNRVKHKPYIKSRDADGRPDEEVADEYWANHIARNDSIIVDVCQGQYKSTLVC 543
           LDGLHEDLNRVKHKPYIKSRDADGRPDEEVADEYWANHIARNDSIIVDVCQGQYKSTLVC
Sbjct: 422 LDGLHEDLNRVKHKPYIKSRDADGRPDEEVADEYWANHIARNDSIIVDVCQGQYKSTLVC 481

Query: 544 PVCNKVSVTFDPFMYLSLPLQSTTTRTMTATLFTSDGSKLPSTLTVTVPKQGRCRDLIQA 603
           PVCNKVSVTFDPFMYLSLPLQSTTTRTMT T+FTSDGSK PSTLTVTVPKQGRCRDLIQA
Sbjct: 482 PVCNKVSVTFDPFMYLSLPLQSTTTRTMTVTVFTSDGSKRPSTLTVTVPKQGRCRDLIQA 541

Query: 604 LNSACLLKHSEKLLLAEVRDHLIHRFLEDPLISLSTIKDDDHIAAYKIPKLTKNTKYLQL 663
           LN  C L+HSEKLLLAEVRDHL+HRFLEDPLISLSTIKDDDHIAAYKIPKLTKN KYLQL
Sbjct: 542 LNGTCSLRHSEKLLLAEVRDHLVHRFLEDPLISLSTIKDDDHIAAYKIPKLTKNAKYLQL 601

Query: 664 VHRRQEQVTSDAHAMSGWRPFGTPLVSVVSCDDPVTKGYIQRIIKRVLSPMAKTGTLMDT 723
           VHR QEQVTSDA  MSGWRPFGTPLVSVVSC+ PV K Y+QRI+KR LSP+AK GTLMDT
Sbjct: 602 VHRSQEQVTSDADIMSGWRPFGTPLVSVVSCEGPVKKDYLQRIVKRALSPLAKIGTLMDT 661

Query: 724 DVFDSSVSHAGEDLHPRDSSIETCTVSISSLMNKDDPKSKGAEPFKLPLQLLNKDNVCID 783
           DV DSS+SHAGEDLH  DSS ETCT S+    N DDPKSK  EPFKLPLQLLN++NVCI+
Sbjct: 662 DVSDSSMSHAGEDLHHHDSSAETCTSSL----NSDDPKSKAMEPFKLPLQLLNEENVCIE 721

Query: 784 LSTGEESVQLPPSSSVLVYIDWSQKLMKKFDTFYLENLPEVFKSGPVMKKARTEPLSLYS 843
           LS+GEE+V+LPPSSSVLVYIDWSQKL+KKFDT YLENLPEVFKSGPVMKKARTEPLSLYS
Sbjct: 722 LSSGEEAVKLPPSSSVLVYIDWSQKLLKKFDTGYLENLPEVFKSGPVMKKARTEPLSLYS 781

Query: 844 CLESFLREEPLVPEDMWFCPQCKERRQASKKLDLWRLPEVLVIHLKRFSYSRSMKHKLET 903
           CLESFLREEPLVPEDMWFCPQCKERRQASKKLDLWRLPEVLVIHLKRFSYSRSMKHKLET
Sbjct: 782 CLESFLREEPLVPEDMWFCPQCKERRQASKKLDLWRLPEVLVIHLKRFSYSRSMKHKLET 841

Query: 904 FVNFPIHDFDLTNYVANKNNSQRQLYELYALTNHYGGMGSGHYTAHIKLIDENRWYNFDD 963
           FVNFPIHDFDLTNYVANKNNSQRQLYELYALTNHYG MGSGHYTAHIKLIDENRWY+FDD
Sbjct: 842 FVNFPIHDFDLTNYVANKNNSQRQLYELYALTNHYGSMGSGHYTAHIKLIDENRWYSFDD 901

Query: 964 SHISLINEEEVKSAAAYVLFYRRVRTEDVSVSNGLQ 999
           SHISLINEEEVKSAAAYVLFYRRV+TEDVS+SNG+Q
Sbjct: 902 SHISLINEEEVKSAAAYVLFYRRVKTEDVSLSNGVQ 933

BLAST of MC03g0310 vs. ExPASy TrEMBL
Match: A0A1S3CJL4 (Ubiquitin carboxyl-terminal hydrolase OS=Cucumis melo OX=3656 GN=LOC103501691 PE=3 SV=1)

HSP 1 Score: 1721 bits (4457), Expect = 0.0
Identity = 858/937 (91.57%), Postives = 889/937 (94.88%), Query Frame = 0

Query: 64  AEVSMCSSGGRTELTPEEERIMIRDIALTAEANTKEGDVFYLITQRWWQHWIEYVNQDQP 123
           AEVSMCSS G TELTPEEERIMIRDIAL AEANTKEGD+FYLITQRWWQHWIEYVNQDQP
Sbjct: 2   AEVSMCSSSGSTELTPEEERIMIRDIALAAEANTKEGDIFYLITQRWWQHWIEYVNQDQP 61

Query: 124 VNANDGSSFAEIYDSFGSSMLKRPANIDNSDLIYDAASEDSSVSIEIHDTLLEGRDYVLL 183
           +NANDGSSFAEIYDSFGSSMLKRPA+IDNSDLIYDAASEDSS  IEIHDTLLEGRDYVLL
Sbjct: 62  INANDGSSFAEIYDSFGSSMLKRPASIDNSDLIYDAASEDSSAGIEIHDTLLEGRDYVLL 121

Query: 184 PQEVWKQLYLWYGGGPTLARKVISAGLSQTELAVEVYPLRLQLLEVGKGDRSTIRISKKE 243
           PQEVW QL LWYGGGP LARKVISAGLSQTEL VEVYPLRLQLLEV KGDRSTIRISKKE
Sbjct: 122 PQEVWNQLCLWYGGGPKLARKVISAGLSQTELTVEVYPLRLQLLEVPKGDRSTIRISKKE 181

Query: 244 TIGELHRRACEIFDLNLEQVCIWDYYGHRKHALMNDMDKTLDDANIQMDQDILVEIINHD 303
           TIGELHRRACEIFDLNLEQVCIWDYYGHRKHALMNDMDKTLDDANIQMDQDILVEI+NH 
Sbjct: 182 TIGELHRRACEIFDLNLEQVCIWDYYGHRKHALMNDMDKTLDDANIQMDQDILVEILNHG 241

Query: 304 NNNALGGCTSYVQDNGTTDKEATSISLESSKSNLIIAGGV-SNKGASKTEVVQSQSLTSS 363
           N+NALGGC SYVQDNGTTDKEATSI LE+SKSNLI AGG+ +NKGASKTEVVQSQ+ TSS
Sbjct: 242 NHNALGGCASYVQDNGTTDKEATSIYLEASKSNLISAGGMPNNKGASKTEVVQSQNPTSS 301

Query: 364 IRESDNTYGQSGVSTRGSSCGLTGLLNLGNTCFMNSAIQCLVHTPEFARYFREDYHQEIN 423
           I+E DNTYGQSGVSTRGSSCGLTGLLNLGNTCFMNSAIQCLVHTPEFARYFREDYHQEIN
Sbjct: 302 IKELDNTYGQSGVSTRGSSCGLTGLLNLGNTCFMNSAIQCLVHTPEFARYFREDYHQEIN 361

Query: 424 RQNPLGMVGELAMAFGDLLRKLWAPGRTSVAPRPFKAKLARFAPQFSGYNQHDSQELLAF 483
            QNPLGMVGELAMAFGDLLRKLWAPGRTSVAPRPFKAKLARFAPQFSGYNQHDSQELLAF
Sbjct: 362 WQNPLGMVGELAMAFGDLLRKLWAPGRTSVAPRPFKAKLARFAPQFSGYNQHDSQELLAF 421

Query: 484 LLDGLHEDLNRVKHKPYIKSRDADGRPDEEVADEYWANHIARNDSIIVDVCQGQYKSTLV 543
           LLDGLHEDLNRVKHKPYIKSRDADGRPDEEVADEYWANHIARNDSIIVDVCQGQYKSTLV
Sbjct: 422 LLDGLHEDLNRVKHKPYIKSRDADGRPDEEVADEYWANHIARNDSIIVDVCQGQYKSTLV 481

Query: 544 CPVCNKVSVTFDPFMYLSLPLQSTTTRTMTATLFTSDGSKLPSTLTVTVPKQGRCRDLIQ 603
           CPVCNKVSVTFDPFMYLSLPLQSTTTRTMT T+FTSDGSK PST TVTVPKQGRCRDLIQ
Sbjct: 482 CPVCNKVSVTFDPFMYLSLPLQSTTTRTMTVTVFTSDGSKRPSTFTVTVPKQGRCRDLIQ 541

Query: 604 ALNSACLLKHSEKLLLAEVRDHLIHRFLEDPLISLSTIKDDDHIAAYKIPKLTKNTKYLQ 663
           ALN  C L+HSEKLLLAEVRDHLIHRFLEDPLISLSTIKDDDHIAAYKIPKLTKN KYLQ
Sbjct: 542 ALNGTCSLRHSEKLLLAEVRDHLIHRFLEDPLISLSTIKDDDHIAAYKIPKLTKNAKYLQ 601

Query: 664 LVHRRQEQVTSDAHAMSGWRPFGTPLVSVVSCDDPVTKGYIQRIIKRVLSPMAKTGTLMD 723
           LVHR QEQVTSDAH MSGWRPFGTPLVSVVSC+ PV K  +QRI+KR LSP+AK GTLMD
Sbjct: 602 LVHRSQEQVTSDAHIMSGWRPFGTPLVSVVSCEGPVKKDCLQRIVKRALSPLAKIGTLMD 661

Query: 724 TDVFDSSVSHAGEDLHPRDSSIETCTVSISSLMNKDDPKSKGAEPFKLPLQLLNKDNVCI 783
            DV DSS+SHAGEDL+  DSS ETCT S+    N DDPKSK  EPFKLPLQLLN++NVCI
Sbjct: 662 NDVSDSSMSHAGEDLNHHDSSAETCTSSL----NSDDPKSKAMEPFKLPLQLLNEENVCI 721

Query: 784 DLSTGEESVQLPPSSSVLVYIDWSQKLMKKFDTFYLENLPEVFKSGPVMKKARTEPLSLY 843
           +LS+GEE+V+LPPS+SVLVYIDWSQKL+KKFDT YLENLPEVFKSGPVMKKARTEPLSLY
Sbjct: 722 ELSSGEEAVRLPPSTSVLVYIDWSQKLLKKFDTGYLENLPEVFKSGPVMKKARTEPLSLY 781

Query: 844 SCLESFLREEPLVPEDMWFCPQCKERRQASKKLDLWRLPEVLVIHLKRFSYSRSMKHKLE 903
           SCLESFLREEPLVPEDMWFCPQCKERRQASKKLDLWRLPEVLVIHLKRFSYSRSMKHKLE
Sbjct: 782 SCLESFLREEPLVPEDMWFCPQCKERRQASKKLDLWRLPEVLVIHLKRFSYSRSMKHKLE 841

Query: 904 TFVNFPIHDFDLTNYVANKNNSQRQLYELYALTNHYGGMGSGHYTAHIKLIDENRWYNFD 963
           TFVNFPIHDFDLTNYVANKNNSQRQLYELYALTNHYG MGSGHYTAHIKLIDENRWY+FD
Sbjct: 842 TFVNFPIHDFDLTNYVANKNNSQRQLYELYALTNHYGSMGSGHYTAHIKLIDENRWYSFD 901

Query: 964 DSHISLINEEEVKSAAAYVLFYRRVRTEDVSVSNGLQ 999
           DSHISLINEEEVKSAAAYVLFYRRV+TEDVS+SNG+Q
Sbjct: 902 DSHISLINEEEVKSAAAYVLFYRRVKTEDVSLSNGVQ 934

BLAST of MC03g0310 vs. ExPASy TrEMBL
Match: A0A6J1KBB7 (Ubiquitin carboxyl-terminal hydrolase OS=Cucurbita maxima OX=3661 GN=LOC111493383 PE=3 SV=1)

HSP 1 Score: 1678 bits (4345), Expect = 0.0
Identity = 842/937 (89.86%), Postives = 875/937 (93.38%), Query Frame = 0

Query: 64  AEVSMCSSGGRTELTPEEERIMIRDIALTAEANTKEGDVFYLITQRWWQHWIEYVNQDQP 123
           AEVSMCSS G TELTPEEERIMIRDIAL AEANTKEGDVFYLITQRWWQHWIEYVNQDQP
Sbjct: 2   AEVSMCSSSGSTELTPEEERIMIRDIALAAEANTKEGDVFYLITQRWWQHWIEYVNQDQP 61

Query: 124 VN-ANDGSSFAEIYDSFGSSMLKRPANIDNSDLIYDAASEDSSVSIEIHDTLLEGRDYVL 183
           V  ANDGSSFAEIYDSFGSSMLKRPA IDNSDLIYDA SEDS + IEIHDTLLEGRDYVL
Sbjct: 62  VGGANDGSSFAEIYDSFGSSMLKRPAIIDNSDLIYDAGSEDSGMGIEIHDTLLEGRDYVL 121

Query: 184 LPQEVWKQLYLWYGGGPTLARKVISAGLSQTELAVEVYPLRLQLLEVGKGDRSTIRISKK 243
           LPQEVW QLYLWYGGGPTLARKVISAGLSQTELAVEVYPLRLQLLEV KGDRST+RISKK
Sbjct: 122 LPQEVWNQLYLWYGGGPTLARKVISAGLSQTELAVEVYPLRLQLLEVPKGDRSTMRISKK 181

Query: 244 ETIGELHRRACEIFDLNLEQVCIWDYYGHRKHALMNDMDKTLDDANIQMDQDILVEIINH 303
           ETIGELHRRACEIFDLNLEQVCIWDYYGHRKHALMNDMDKTLDDAN+QMDQDILVEIINH
Sbjct: 182 ETIGELHRRACEIFDLNLEQVCIWDYYGHRKHALMNDMDKTLDDANLQMDQDILVEIINH 241

Query: 304 DNNNALGGCTSYVQDNGTTDKEATSISLESSKSNLIIAGGVSNKGASKTEVVQSQSLTSS 363
            NNNA  GC SYVQDNGT DKEATSISLE SKSNL +AGG+SNKG SKTE VQSQ+LTSS
Sbjct: 242 GNNNASDGCASYVQDNGTADKEATSISLEPSKSNLNVAGGMSNKGMSKTEAVQSQNLTSS 301

Query: 364 IRESDNTYGQSGVSTRGSSCGLTGLLNLGNTCFMNSAIQCLVHTPEFARYFREDYHQEIN 423
           IRE DN  G +GVSTRGSSCGLTGLLNLGNTCFMNSAIQCLVHTPEFARYFREDYHQEIN
Sbjct: 302 IRELDNASGHTGVSTRGSSCGLTGLLNLGNTCFMNSAIQCLVHTPEFARYFREDYHQEIN 361

Query: 424 RQNPLGMVGELAMAFGDLLRKLWAPGRTSVAPRPFKAKLARFAPQFSGYNQHDSQELLAF 483
            QNPLGMVGELAMAFGDLLRKLWAPGRTSVAPRPFKAKLARFAPQFSGYNQHDSQELLAF
Sbjct: 362 WQNPLGMVGELAMAFGDLLRKLWAPGRTSVAPRPFKAKLARFAPQFSGYNQHDSQELLAF 421

Query: 484 LLDGLHEDLNRVKHKPYIKSRDADGRPDEEVADEYWANHIARNDSIIVDVCQGQYKSTLV 543
           LLDGLHEDLNRVKHKPYIKSRDADGRPDEEVADEYWANHIARNDSIIVDVCQGQYKSTLV
Sbjct: 422 LLDGLHEDLNRVKHKPYIKSRDADGRPDEEVADEYWANHIARNDSIIVDVCQGQYKSTLV 481

Query: 544 CPVCNKVSVTFDPFMYLSLPLQSTTTRTMTATLFTSDGSKLPSTLTVTVPKQGRCRDLIQ 603
           CPVCNKVSVTFDPFMYLSLPLQSTTT TMT T+F+SDGSKLPSTLTVTVPKQGRCRDLIQ
Sbjct: 482 CPVCNKVSVTFDPFMYLSLPLQSTTTCTMTVTVFSSDGSKLPSTLTVTVPKQGRCRDLIQ 541

Query: 604 ALNSACLLKHSEKLLLAEVRDHLIHRFLEDPLISLSTIKDDDHIAAYKIPKLTKNTKYLQ 663
           ALN  C LK +EKLLLAEVRDHLIHRFLEDPLISLSTIKDD HI AYKIPKLTKNTKY+Q
Sbjct: 542 ALNGTCSLKQNEKLLLAEVRDHLIHRFLEDPLISLSTIKDDSHIVAYKIPKLTKNTKYIQ 601

Query: 664 LVHRRQEQVTSDAHAMSGWRPFGTPLVSVVSCDDPVTKGYIQRIIKRVLSPMAKTGTLMD 723
           L+HR QEQVTSDAH MSGWR FGTPLVSVVS D PV K Y++RI+K+VLSPMAK GT MD
Sbjct: 602 LIHRSQEQVTSDAHKMSGWRAFGTPLVSVVSPDGPVKKVYLERIVKKVLSPMAKIGTSMD 661

Query: 724 TDVFDSSVSHAGEDLHPRDSSIETCTVSISSLMNKDDPKSKGAEPFKLPLQLLNKDNVCI 783
           TDV DS++SHAGED      S ETCT S    MN +DPKSK  EP +LPLQLLN+DNVCI
Sbjct: 662 TDVTDSTMSHAGED------STETCTSS----MNSNDPKSKVLEPLELPLQLLNEDNVCI 721

Query: 784 DLSTGEESVQLPPSSSVLVYIDWSQKLMKKFDTFYLENLPEVFKSGPVMKKARTEPLSLY 843
           DLS+GEE+VQLP SSSV VY+DWSQKL+KKFDT +LENLPEVFKSGPV+KKARTEPLSLY
Sbjct: 722 DLSSGEEAVQLPSSSSVTVYLDWSQKLLKKFDTCHLENLPEVFKSGPVLKKARTEPLSLY 781

Query: 844 SCLESFLREEPLVPEDMWFCPQCKERRQASKKLDLWRLPEVLVIHLKRFSYSRSMKHKLE 903
           SCLESFLREEPLVPEDMWFCP+CKERRQASKKLDLWRLPEVLVIHLKRFSYSRSMKHKLE
Sbjct: 782 SCLESFLREEPLVPEDMWFCPECKERRQASKKLDLWRLPEVLVIHLKRFSYSRSMKHKLE 841

Query: 904 TFVNFPIHDFDLTNYVANKNNSQRQLYELYALTNHYGGMGSGHYTAHIKLIDENRWYNFD 963
           TFVNFPIHDF+LTNYVANKN+SQRQLYELYALTNHYGGMGSGHYTAHIKLI+ENRWYNFD
Sbjct: 842 TFVNFPIHDFELTNYVANKNSSQRQLYELYALTNHYGGMGSGHYTAHIKLIEENRWYNFD 901

Query: 964 DSHISLINEEEVKSAAAYVLFYRRVRTEDVSVSNGLQ 999
           DSHISLINEEEVKSAAAYVLFYRRV+TEDVSVSNG+Q
Sbjct: 902 DSHISLINEEEVKSAAAYVLFYRRVKTEDVSVSNGVQ 928

BLAST of MC03g0310 vs. ExPASy TrEMBL
Match: A0A6J1GA42 (Ubiquitin carboxyl-terminal hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111452171 PE=3 SV=1)

HSP 1 Score: 1677 bits (4342), Expect = 0.0
Identity = 843/937 (89.97%), Postives = 875/937 (93.38%), Query Frame = 0

Query: 64  AEVSMCSSGGRTELTPEEERIMIRDIALTAEANTKEGDVFYLITQRWWQHWIEYVNQDQP 123
           AEVSMCSS G TELTPEEERIMIRDIAL AEANTKEGDVFYLITQRWWQHWIEYVNQDQP
Sbjct: 2   AEVSMCSSSGSTELTPEEERIMIRDIALAAEANTKEGDVFYLITQRWWQHWIEYVNQDQP 61

Query: 124 VN-ANDGSSFAEIYDSFGSSMLKRPANIDNSDLIYDAASEDSSVSIEIHDTLLEGRDYVL 183
           V  ANDGSSFAEIYDSFGSSMLKRPA IDNSDLIYDAASEDS + IEIHDTLLEGRDYVL
Sbjct: 62  VGGANDGSSFAEIYDSFGSSMLKRPAIIDNSDLIYDAASEDSGMGIEIHDTLLEGRDYVL 121

Query: 184 LPQEVWKQLYLWYGGGPTLARKVISAGLSQTELAVEVYPLRLQLLEVGKGDRSTIRISKK 243
           LPQEVW QLYLWYGGGPTLARKVISAGLSQTELAVEVYPLRLQLLEV KGDRST+RISKK
Sbjct: 122 LPQEVWNQLYLWYGGGPTLARKVISAGLSQTELAVEVYPLRLQLLEVPKGDRSTMRISKK 181

Query: 244 ETIGELHRRACEIFDLNLEQVCIWDYYGHRKHALMNDMDKTLDDANIQMDQDILVEIINH 303
           ETIGELHRRACEIF LNLEQVCIWDYYGHRKHALMNDMDKTLDDAN+QMDQDILVEII+H
Sbjct: 182 ETIGELHRRACEIFGLNLEQVCIWDYYGHRKHALMNDMDKTLDDANLQMDQDILVEIISH 241

Query: 304 DNNNALGGCTSYVQDNGTTDKEATSISLESSKSNLIIAGGVSNKGASKTEVVQSQSLTSS 363
            NNNA  GC SYVQDNGT DKEATSISLE SKSNL +AGG+SNKG SKTEVVQSQ+ TSS
Sbjct: 242 GNNNASDGCASYVQDNGTADKEATSISLEPSKSNLNVAGGMSNKGMSKTEVVQSQNQTSS 301

Query: 364 IRESDNTYGQSGVSTRGSSCGLTGLLNLGNTCFMNSAIQCLVHTPEFARYFREDYHQEIN 423
           IRE DN  G +GVSTRGSSCGLTGLLNLGNTCFMNSAIQCLVHTPEFARYFREDYHQEIN
Sbjct: 302 IRELDNASGHTGVSTRGSSCGLTGLLNLGNTCFMNSAIQCLVHTPEFARYFREDYHQEIN 361

Query: 424 RQNPLGMVGELAMAFGDLLRKLWAPGRTSVAPRPFKAKLARFAPQFSGYNQHDSQELLAF 483
            QNPLGMVGELAMAFGDLLRKLWAPGRTSVAPRPFKAKLARFAPQFSGYNQHDSQELLAF
Sbjct: 362 WQNPLGMVGELAMAFGDLLRKLWAPGRTSVAPRPFKAKLARFAPQFSGYNQHDSQELLAF 421

Query: 484 LLDGLHEDLNRVKHKPYIKSRDADGRPDEEVADEYWANHIARNDSIIVDVCQGQYKSTLV 543
           LLDGLHEDLNRVKHKPYIKSRDADGRPDEEVADEYWANHIARNDSIIVDVCQGQYKSTLV
Sbjct: 422 LLDGLHEDLNRVKHKPYIKSRDADGRPDEEVADEYWANHIARNDSIIVDVCQGQYKSTLV 481

Query: 544 CPVCNKVSVTFDPFMYLSLPLQSTTTRTMTATLFTSDGSKLPSTLTVTVPKQGRCRDLIQ 603
           CPVCNKVSVTFDPFMYLSLPLQSTTT TMT T+FTSDGSKLPSTLTVTVPKQGRCRDLIQ
Sbjct: 482 CPVCNKVSVTFDPFMYLSLPLQSTTTCTMTVTVFTSDGSKLPSTLTVTVPKQGRCRDLIQ 541

Query: 604 ALNSACLLKHSEKLLLAEVRDHLIHRFLEDPLISLSTIKDDDHIAAYKIPKLTKNTKYLQ 663
           ALN  C LK +EKLLLAEVRDHLIHRFLEDPLISLSTIKDD HI AYKIPKLTKNTKY+Q
Sbjct: 542 ALNGTCSLKQNEKLLLAEVRDHLIHRFLEDPLISLSTIKDDSHIVAYKIPKLTKNTKYIQ 601

Query: 664 LVHRRQEQVTSDAHAMSGWRPFGTPLVSVVSCDDPVTKGYIQRIIKRVLSPMAKTGTLMD 723
           LVHR QEQVTSDAH MSGWR FGTPLVSVVS D PV K Y++RI+KRVLSP+AK GTLMD
Sbjct: 602 LVHRSQEQVTSDAHKMSGWRAFGTPLVSVVSSDGPVKKVYLERIVKRVLSPLAKIGTLMD 661

Query: 724 TDVFDSSVSHAGEDLHPRDSSIETCTVSISSLMNKDDPKSKGAEPFKLPLQLLNKDNVCI 783
           TDV DS++SHAGED      S ETCT S    MN +DPKSK  EP +LPLQLLN+DNVCI
Sbjct: 662 TDVTDSTMSHAGED------SAETCTSS----MNSNDPKSKVMEPLELPLQLLNEDNVCI 721

Query: 784 DLSTGEESVQLPPSSSVLVYIDWSQKLMKKFDTFYLENLPEVFKSGPVMKKARTEPLSLY 843
           DLS+GEE+VQLP SSSV VY+DWSQKL+KKFDT +LENLPEVFKSGPV+KKARTEPLSLY
Sbjct: 722 DLSSGEEAVQLPSSSSVTVYLDWSQKLLKKFDTCHLENLPEVFKSGPVLKKARTEPLSLY 781

Query: 844 SCLESFLREEPLVPEDMWFCPQCKERRQASKKLDLWRLPEVLVIHLKRFSYSRSMKHKLE 903
           SCLESFLREEPLVPEDMWFCP+CKERRQASKKLDLWRLPEVLVIHLKRFSYSRSMKHKLE
Sbjct: 782 SCLESFLREEPLVPEDMWFCPECKERRQASKKLDLWRLPEVLVIHLKRFSYSRSMKHKLE 841

Query: 904 TFVNFPIHDFDLTNYVANKNNSQRQLYELYALTNHYGGMGSGHYTAHIKLIDENRWYNFD 963
           TFVNFPIHDF+LTNYVANKN+SQRQLYELYALTNHYG MGSGHYTAHIKLI+ENRWYNFD
Sbjct: 842 TFVNFPIHDFELTNYVANKNSSQRQLYELYALTNHYGSMGSGHYTAHIKLIEENRWYNFD 901

Query: 964 DSHISLINEEEVKSAAAYVLFYRRVRTEDVSVSNGLQ 999
           DSHISLINEEEVKSAAAYVLFYRRV+TEDVSVSNG+Q
Sbjct: 902 DSHISLINEEEVKSAAAYVLFYRRVKTEDVSVSNGVQ 928

BLAST of MC03g0310 vs. TAIR 10
Match: AT2G40930.1 (ubiquitin-specific protease 5 )

HSP 1 Score: 1255.7 bits (3248), Expect = 0.0e+00
Identity = 644/927 (69.47%), Postives = 743/927 (80.15%), Query Frame = 0

Query: 64  AEVSMCSSGGRTELTPEEERIMIRDIALTAEANTKEGDVFYLITQRWWQHWIEYVNQDQP 123
           AEVSM SS   T+L+PEEER+ IRDIA+ AEAN+KEGD FYLITQRWWQ WIEYVNQDQP
Sbjct: 2   AEVSMGSSSSSTDLSPEEERVFIRDIAIAAEANSKEGDTFYLITQRWWQEWIEYVNQDQP 61

Query: 124 VNANDGSSFAEIYDSFGSSMLKRPANIDNSDLIYDAASEDSSVSIEIHDTLLEGRDYVLL 183
            N NDGSS +E  DS GSS LK+P+ IDNSDLIYD++ ED S + EI +TL EGRDYVLL
Sbjct: 62  CNTNDGSSLSEHCDSPGSSTLKKPSRIDNSDLIYDSSLEDPSNTSEIIETLQEGRDYVLL 121

Query: 184 PQEVWKQLYLWYGGGPTLARKVISAGLSQTELAVEVYPLRLQLLEVGKGDRSTIRISKKE 243
           PQEVW QL  WYGGGPTLAR+VIS+GLSQTELAVEVYPLRLQLL + K D S IRISKKE
Sbjct: 122 PQEVWNQLRSWYGGGPTLARRVISSGLSQTELAVEVYPLRLQLLLMPKSDHSAIRISKKE 181

Query: 244 TIGELHRRACEIFDLNLEQVCIWDYYGHRKHALMNDMDKTLDDANIQMDQDILVEIINHD 303
           TI ELHRRACEIFDL+ E V IWDYYGH+K++LMND+DKTLDDAN+QMDQDILVE++  D
Sbjct: 182 TIRELHRRACEIFDLDSEHVRIWDYYGHQKYSLMNDLDKTLDDANLQMDQDILVEVL--D 241

Query: 304 NNNALGGC-TSYVQDNGTTDKEATSISLESSKSNLIIAGGV-SNKGASKTEVVQ-SQSLT 363
            N  L        Q+NG  D ++TSI +E SKS+L  AGG  S++ A +T  V+ SQS  
Sbjct: 242 INGTLSSAHIQSAQENGLVDGDSTSILIEPSKSSLAAAGGFSSSRNAFRTGSVEVSQSF- 301

Query: 364 SSIRESDNTYGQSGVSTRGSSCGLTGLLNLGNTCFMNSAIQCLVHTPEFARYFREDYHQE 423
                 DNTY  +GV+TRGS+ GLTGLLNLGNTCFMNSAIQCLVHTPEFA YF+EDYHQE
Sbjct: 302 ------DNTYSSTGVTTRGSTAGLTGLLNLGNTCFMNSAIQCLVHTPEFASYFQEDYHQE 361

Query: 424 INRQNPLGMVGELAMAFGDLLRKLWAPGRTSVAPRPFKAKLARFAPQFSGYNQHDSQELL 483
           IN QNPLGMVGELA+AFGDLLRKLWAPGRT +APRPFKAKLARFAPQFSGYNQHDSQELL
Sbjct: 362 INWQNPLGMVGELALAFGDLLRKLWAPGRTPIAPRPFKAKLARFAPQFSGYNQHDSQELL 421

Query: 484 AFLLDGLHEDLNRVKHKPYIKSRDADGRPDEEVADEYWANHIARNDSIIVDVCQGQYKST 543
           AFLLDGLHEDLNRVKHKPYI SRDADGRPDEEVADE+W NHIARNDSIIVDVCQGQYKST
Sbjct: 422 AFLLDGLHEDLNRVKHKPYINSRDADGRPDEEVADEFWKNHIARNDSIIVDVCQGQYKST 481

Query: 544 LVCPVCNKVSVTFDPFMYLSLPLQSTTTRTMTATLFTSDGSKLPSTLTVTVPKQGRCRDL 603
           LVCP+CNKVSVTFDPFMYLSLPLQ  TTR +T T+F+ D + LPST+TV V KQGRCRDL
Sbjct: 482 LVCPICNKVSVTFDPFMYLSLPLQFNTTRAITVTVFSCDKTALPSTITVNVSKQGRCRDL 541

Query: 604 IQALNSACLLKHSEKLLLAEVRDHLIHRFLEDPLISLSTIKDDDHIAAYKIPKLTKNTKY 663
           IQAL +AC LK SE+L LAE+R++ IHR  EDPLI LS+IKDDDH+AAYK+ K ++NT  
Sbjct: 542 IQALTNACSLKQSEELKLAEIRNNFIHRLFEDPLIPLSSIKDDDHLAAYKLSKSSENTTL 601

Query: 664 LQLVHRRQEQVTSDAHAMSGWRPFGTPLVSVVSCDDPVTKGYIQRIIKRVLSPMAKTGTL 723
           L+LV RR++Q   +  +    +P GTPL+S  SC D +TKG I  +++ +LSP  +  ++
Sbjct: 602 LRLVLRRRDQKAGERESTVQLKPCGTPLLSSASCGDALTKGKIHCLVQNMLSPFRREESV 661

Query: 724 MDTDVFDSSVSHAGEDLHPRDSSIETCTVSISSLMNKDDPKSKGAEPFKLPLQLLNKDNV 783
                 DSS+    E    R ++ E     +  L       S      KL LQL+++DN 
Sbjct: 662 GKKGNSDSSIP---ERRSARFNNTEE-EDKVGGLKKAKKSNSSDLGASKLSLQLIDEDNK 721

Query: 784 CIDLSTGE-ESVQLPPSSSVLVYIDWSQKLMKKFDTFYLENLPEVFKSGPVMKKARTEPL 843
            I+L   E E+++LP S++V +Y+DW+ +L   +D   LE+LPEV K GP  KKAR+EPL
Sbjct: 722 TINLPDNEAEAMKLPSSATVTIYLDWTPELSGMYDITCLESLPEVLKYGPTTKKARSEPL 781

Query: 844 SLYSCLESFLREEPLVPEDMWFCPQCKERRQASKKLDLWRLPEVLVIHLKRFSYSRSMKH 903
           SLY+CLE+FLREEPLVP++MWFCPQC ERRQASKKLDLWRLPEVLVIHLKRFSYSRSMKH
Sbjct: 782 SLYACLEAFLREEPLVPDEMWFCPQCNERRQASKKLDLWRLPEVLVIHLKRFSYSRSMKH 841

Query: 904 KLETFVNFPIHDFDLTNYVANKNNSQRQLYELYALTNHYGGMGSGHYTAHIKLIDENRWY 963
           KLETFVNFPIHD DLT YVANKN SQ QLYELYALTNHYGGMGSGHYTAHIKL+D++RWY
Sbjct: 842 KLETFVNFPIHDLDLTKYVANKNLSQPQLYELYALTNHYGGMGSGHYTAHIKLLDDSRWY 901

Query: 964 NFDDSHISLINEEEVKSAAAYVLFYRR 987
           NFDDSHIS INE++VKS AAYVLFYRR
Sbjct: 902 NFDDSHISHINEDDVKSGAAYVLFYRR 915

BLAST of MC03g0310 vs. TAIR 10
Match: AT4G10570.1 (ubiquitin-specific protease 9 )

HSP 1 Score: 851.7 bits (2199), Expect = 6.1e-247
Identity = 446/917 (48.64%), Postives = 611/917 (66.63%), Query Frame = 0

Query: 78  TPEEERIMIRDIALTAEANTKEGDVFYLITQRWWQHWIEYVNQDQPVNANDGSSFAEIYD 137
           TPEEE+ ++ ++   +E N K+G+++++I++RW+  W EYV      +AN+ S+      
Sbjct: 20  TPEEEKRIVSELTSESEDNLKQGNLYFVISKRWYTSWQEYVEN----SANECSTGE---- 79

Query: 138 SFGSSMLKRPANIDNSDLIYDAASEDSSVS-IEIHDTLLEGRDYVLLPQEVWKQLYLWYG 197
              SS   RP  IDN D+I      DS ++  ++   L+EG DYVL+P++VWK+L  WY 
Sbjct: 80  ---SSEAPRPGPIDNHDII----ESDSDINDPQLRRLLVEGEDYVLVPKQVWKRLVEWYS 139

Query: 198 GGPTLARKVISAGLSQTELAVEVYPLRLQLLEVGKGDRSTIRISKKETIGELHRRACEIF 257
           GGP + RK+I  G      +VEVYPL L L +     R+ IR+ K+ +I EL+ + C + 
Sbjct: 140 GGPPIERKLICQGFYTRSYSVEVYPLCLMLTDGRDESRTVIRLGKQASIRELYEKVCAMT 199

Query: 258 DLNLEQVCIWDYYGHRKHALMNDMD-KTLDDANIQMDQDILVEIINHDNNNALGGCTSYV 317
            +  E+  IWDY+  RK+ L++ +  K+L+++++ MDQDILVE+         G  +S  
Sbjct: 200 GVPQEKAHIWDYFDKRKNGLLDPLSYKSLEESSLHMDQDILVEVD--------GLSSSSQ 259

Query: 318 QDNGTTDKEATSISLESSKSNLIIAGGVSNKGASKTEVVQSQSLTSSIRESDNTYGQSGV 377
               +T  E   + LE S+S + IAGG +      T    + SL   I   D+      +
Sbjct: 260 SAMSSTGNELALVPLEPSRSIVTIAGGPTLSNGHST--TSNFSLFPRITSEDDGRDSLSI 319

Query: 378 STRGSSCGLTGLLNLGNTCFMNSAIQCLVHTPEFARYFREDYHQEINRQNPLGMVGELAM 437
             +G   GL GL NLGNTCFMNSA+QCL HTP    YF +DY  +INR NPLGM GELA+
Sbjct: 320 LGKGEKGGLAGLSNLGNTCFMNSALQCLAHTPPIVEYFLQDYSDDINRDNPLGMCGELAI 379

Query: 438 AFGDLLRKLWAPGRTSVAPRPFKAKLARFAPQFSGYNQHDSQELLAFLLDGLHEDLNRVK 497
           AFGDLL+KLW+ GR +VAPR FK KLARFAPQFSGYNQHDSQELLAFLLDGLHEDLN+VK
Sbjct: 380 AFGDLLKKLWSSGRNAVAPRAFKTKLARFAPQFSGYNQHDSQELLAFLLDGLHEDLNKVK 439

Query: 498 HKPYIKSRDADGRPDEEVADEYWANHIARNDSIIVDVCQGQYKSTLVCPVCNKVSVTFDP 557
            KPYI+ +D+D RPD+EVA+E W  H ARNDS+IVDVCQGQYKSTLVCPVC K+S+TFDP
Sbjct: 440 RKPYIELKDSDSRPDDEVAEELWNYHKARNDSVIVDVCQGQYKSTLVCPVCGKISITFDP 499

Query: 558 FMYLSLPLQSTTTRTMTATLFTSDGSKLPSTLTVTVPKQGRCRDLIQALNSACLLKHSEK 617
           FMYLS+PL ST TR+MT T+F  DGS+LP   TV VPKQG  RDLI AL +AC L   E 
Sbjct: 500 FMYLSVPLPSTLTRSMTITVFYCDGSRLPMPYTVIVPKQGSIRDLITALGTACCLAEDES 559

Query: 618 LLLAEVRDHLIHRFLEDPLISLSTIKDDDHIAAYKIPKLTKNTK--YLQLVHRRQEQVTS 677
           LLLAEV DH I R+ E PL SLS IKDD+HI AY++ ++ K ++   L+++H  QE+   
Sbjct: 560 LLLAEVYDHKIFRYFEIPLDSLSAIKDDEHIVAYRLNQIPKGSRKAKLEILHGGQERAVL 619

Query: 678 DAHAMSGWRPFGTPLVSVVSCDDPVTKGYIQRIIKRVLSPMAKTGTLMDTDVFDSSVSHA 737
           D+   S  + FGTP V+ V+  +P++   I  +I   LSP+ K           S + + 
Sbjct: 620 DSVRGSDVKLFGTPFVTYVN-TEPLSGTDIDAVISGFLSPLHKVHA-------PSKIHNG 679

Query: 738 GEDLHPRDSSIETCTVSISSLMNKDDPKSKGAEPFKLPLQLLNKDNVCIDLSTGEESVQL 797
            ++ H  D++++      S +++  D +   A   +L  ++   D   +++   +    +
Sbjct: 680 SDNGHLADATVD----QASGILSSPDTEIDNASDRELSFRIFLTDERGLNIKPLQSESSI 739

Query: 798 PPSSSVLVYIDWSQKLMKKFDTFYLENLPEVFKSGPVMKKARTEPLSLYSCLESFLREEP 857
            P +   V ++W++   +++D+ YL +LPEV K+    KK R E +SL+SCLE+FL EEP
Sbjct: 740 SPGTVTRVLVEWNEGEHERYDSSYLSDLPEVHKTSFSAKKTRQESISLFSCLEAFLAEEP 799

Query: 858 LVPEDMWFCPQCKERRQASKKLDLWRLPEVLVIHLKRFSYSRSMKHKLETFVNFPIHDFD 917
           L P+DMWFCP CKE RQA+KKLDLW+LP++LV HLKRF+YSR +K+K++TFVNFP+HD D
Sbjct: 800 LGPDDMWFCPSCKEHRQANKKLDLWKLPDILVFHLKRFTYSRYLKNKIDTFVNFPVHDLD 859

Query: 918 LTNYVANKNNSQRQLYELYALTNHYGGMGSGHYTAHIKLIDENRWYNFDDSHISLINEEE 977
           L+ YV NK N Q  LYELYA++NHYGG+G GHYTA+ KLID+N+WY+FDDSH+S +NE E
Sbjct: 860 LSKYVKNK-NGQSYLYELYAVSNHYGGLGGGHYTAYAKLIDDNKWYHFDDSHVSSVNESE 898

Query: 978 VKSAAAYVLFYRRVRTE 991
           ++++AAYVLFYRRVR+E
Sbjct: 920 IRNSAAYVLFYRRVRSE 898

BLAST of MC03g0310 vs. TAIR 10
Match: AT4G10590.2 (ubiquitin-specific protease 10 )

HSP 1 Score: 848.6 bits (2191), Expect = 5.2e-246
Identity = 449/916 (49.02%), Postives = 608/916 (66.38%), Query Frame = 0

Query: 78  TPEEERIMIRDIALTAEANTKEGDVFYLITQRWWQHWIEYVNQDQPVNANDGSSFAEIYD 137
           TPEEE+ ++ ++   +E N KEG+++++I++RW+  W +YV Q             + Y 
Sbjct: 20  TPEEEKRIVSELITESEDNLKEGNLYFVISKRWYTSWEKYVEQS-----------TKEYI 79

Query: 138 SFGSSMLKRPANIDNSDLIYDAASEDSSVSIEIHDTLLEGRDYVLLPQEVWKQLYLWYGG 197
           S  SS   RP  IDN D+I    SE      ++   L+E  DYVL+PQEVWK+L  WY G
Sbjct: 80  SGESSEASRPGPIDNHDII---ESESDVNDPQLRRLLMERVDYVLVPQEVWKRLVEWYSG 139

Query: 198 GPTLARKVISAGLSQTELAVEVYPLRLQLLEVGKGDRSTIRISKKETIGELHRRACEIFD 257
           GP + RK+I  G      +VEVYPL L L +     R+ IR+ K+ +I EL+ + C +  
Sbjct: 140 GPPIERKLICQGFYTRSYSVEVYPLCLMLTDGRDESRTVIRLGKQASIRELYEKVCALTG 199

Query: 258 LNLEQVCIWDYYGHRKHALMNDMD-KTLDDANIQMDQDILVEIINHDNNNALGGCTSYVQ 317
           +  E+  IWDY+  RK+ L++ +  K+L+++++ MDQDIL+E+          G +S   
Sbjct: 200 VPQEKAHIWDYFDKRKNGLLDSLSYKSLEESSLHMDQDILLEV---------DGSSSSQS 259

Query: 318 DNGTTDKEATSISLESSKSNLIIAGGVSNKGASKTEVVQSQSLTSSIRESDNTYGQSGVS 377
              +T  E   + LE S+S++ IAGG +      T    + SL   I   D+      + 
Sbjct: 260 AMSSTGNELALVPLEPSRSSVTIAGGPTLSNGHST--TSNFSLFPRITSEDDGSNSLSIL 319

Query: 378 TRGSSCGLTGLLNLGNTCFMNSAIQCLVHTPEFARYFREDYHQEINRQNPLGMVGELAMA 437
            +G   GL GL NLGNTCFMNSA+QCL HTP    YF +DY  +INR NPLGM GELA+A
Sbjct: 320 GKGEKGGLAGLSNLGNTCFMNSALQCLAHTPPIVEYFLQDYSDDINRDNPLGMCGELAIA 379

Query: 438 FGDLLRKLWAPGRTSVAPRPFKAKLARFAPQFSGYNQHDSQELLAFLLDGLHEDLNRVKH 497
           FGDLL+KLW+ GR SVAPR FK KLARFAPQFSGYNQHDSQELLAFLLDGLHEDLN+VK 
Sbjct: 380 FGDLLKKLWSSGRNSVAPRAFKTKLARFAPQFSGYNQHDSQELLAFLLDGLHEDLNKVKR 439

Query: 498 KPYIKSRDADGRPDEEVADEYWANHIARNDSIIVDVCQGQYKSTLVCPVCNKVSVTFDPF 557
           KPYI+ +D+D RPD+EVA+E W  H ARNDS+IVDVCQGQYKSTLVCP C K+S+TFDPF
Sbjct: 440 KPYIELKDSDSRPDDEVAEELWNYHKARNDSVIVDVCQGQYKSTLVCPACGKISITFDPF 499

Query: 558 MYLSLPLQSTTTRTMTATLFTSDGSKLPSTLTVTVPKQGRCRDLIQALNSACLLKHSEKL 617
           MYLS+PL ST TR+MT T+F  DGS LP   TV VPK G  RDLI AL +ACLL   E L
Sbjct: 500 MYLSVPLPSTLTRSMTVTVFYCDGSHLPMPYTVIVPKNGSIRDLITALGTACLLAEDESL 559

Query: 618 LLAEVRDHLIHRFLEDPLISLSTIKDDDHIAAYKIPKLTKNT--KYLQLVHRRQEQVTSD 677
           LLAEV DH I ++ E+PL SLS+IKDD+HI AY++ ++ K +    L+++H  Q++   +
Sbjct: 560 LLAEVYDHKIFKYFENPLDSLSSIKDDEHIVAYRLNQMPKGSGKAKLEILHGGQKRPILE 619

Query: 678 AHAMSGWRPFGTPLVSVVSCDDPVTKGYIQRIIKRVLSPMAKTGTLMDTDVFDSSVSHAG 737
           +      + FGTP V+ V+  +P++   I  ++ R LSP+ K           S + +  
Sbjct: 620 SVRGRDVKLFGTPFVTYVN-TEPLSGADIDAVLSRFLSPLHKVHA-------PSKIHNGS 679

Query: 738 EDLHPRDSSIETCTVSISSLMNKDDPKSKGAEPFKLPLQLLNKDNVCIDLSTGEESVQLP 797
           E+ H  D++++  +  +SS   + D  S     F+  + L ++  +       E S+ L 
Sbjct: 680 ENGHLPDATVDEASEILSSPDTEIDDASDRELSFR--IFLTDERGLNFKPLQSESSISLG 739

Query: 798 PSSSVLVYIDWSQKLMKKFDTFYLENLPEVFKSGPVMKKARTEPLSLYSCLESFLREEPL 857
            ++ VLV  +W++   +++D+ YL +LPEV K+    KK R E +SL+SCLE+FL EEPL
Sbjct: 740 IATRVLV--EWNEGEHERYDSSYLSDLPEVHKTSFSAKKTRQESISLFSCLEAFLAEEPL 799

Query: 858 VPEDMWFCPQCKERRQASKKLDLWRLPEVLVIHLKRFSYSRSMKHKLETFVNFPIHDFDL 917
            P+DMWFCP CKE RQA+KKLDLW+LP++LV HLKRF+YSR +K+K++TFVNFP+HD DL
Sbjct: 800 GPDDMWFCPSCKEHRQANKKLDLWKLPDILVFHLKRFTYSRYLKNKIDTFVNFPVHDLDL 859

Query: 918 TNYVANKNNSQRQLYELYALTNHYGGMGSGHYTAHIKLIDENRWYNFDDSHISLINEEEV 977
           + YV NKN+ Q  LYELYA++NHYGG+G GHYTA+ KLID+N WY+FDDSH+S +NE E+
Sbjct: 860 SKYVKNKND-QSYLYELYAVSNHYGGLGGGHYTAYAKLIDDNEWYHFDDSHVSSVNESEI 897

Query: 978 KSAAAYVLFYRRVRTE 991
           K++AAYVLFYRRVR+E
Sbjct: 920 KNSAAYVLFYRRVRSE 897

BLAST of MC03g0310 vs. TAIR 10
Match: AT4G10590.1 (ubiquitin-specific protease 10 )

HSP 1 Score: 848.6 bits (2191), Expect = 5.2e-246
Identity = 449/916 (49.02%), Postives = 608/916 (66.38%), Query Frame = 0

Query: 78  TPEEERIMIRDIALTAEANTKEGDVFYLITQRWWQHWIEYVNQDQPVNANDGSSFAEIYD 137
           TPEEE+ ++ ++   +E N KEG+++++I++RW+  W +YV Q             + Y 
Sbjct: 20  TPEEEKRIVSELITESEDNLKEGNLYFVISKRWYTSWEKYVEQS-----------TKEYI 79

Query: 138 SFGSSMLKRPANIDNSDLIYDAASEDSSVSIEIHDTLLEGRDYVLLPQEVWKQLYLWYGG 197
           S  SS   RP  IDN D+I    SE      ++   L+E  DYVL+PQEVWK+L  WY G
Sbjct: 80  SGESSEASRPGPIDNHDII---ESESDVNDPQLRRLLMERVDYVLVPQEVWKRLVEWYSG 139

Query: 198 GPTLARKVISAGLSQTELAVEVYPLRLQLLEVGKGDRSTIRISKKETIGELHRRACEIFD 257
           GP + RK+I  G      +VEVYPL L L +     R+ IR+ K+ +I EL+ + C +  
Sbjct: 140 GPPIERKLICQGFYTRSYSVEVYPLCLMLTDGRDESRTVIRLGKQASIRELYEKVCALTG 199

Query: 258 LNLEQVCIWDYYGHRKHALMNDMD-KTLDDANIQMDQDILVEIINHDNNNALGGCTSYVQ 317
           +  E+  IWDY+  RK+ L++ +  K+L+++++ MDQDIL+E+          G +S   
Sbjct: 200 VPQEKAHIWDYFDKRKNGLLDSLSYKSLEESSLHMDQDILLEV---------DGSSSSQS 259

Query: 318 DNGTTDKEATSISLESSKSNLIIAGGVSNKGASKTEVVQSQSLTSSIRESDNTYGQSGVS 377
              +T  E   + LE S+S++ IAGG +      T    + SL   I   D+      + 
Sbjct: 260 AMSSTGNELALVPLEPSRSSVTIAGGPTLSNGHST--TSNFSLFPRITSEDDGSNSLSIL 319

Query: 378 TRGSSCGLTGLLNLGNTCFMNSAIQCLVHTPEFARYFREDYHQEINRQNPLGMVGELAMA 437
            +G   GL GL NLGNTCFMNSA+QCL HTP    YF +DY  +INR NPLGM GELA+A
Sbjct: 320 GKGEKGGLAGLSNLGNTCFMNSALQCLAHTPPIVEYFLQDYSDDINRDNPLGMCGELAIA 379

Query: 438 FGDLLRKLWAPGRTSVAPRPFKAKLARFAPQFSGYNQHDSQELLAFLLDGLHEDLNRVKH 497
           FGDLL+KLW+ GR SVAPR FK KLARFAPQFSGYNQHDSQELLAFLLDGLHEDLN+VK 
Sbjct: 380 FGDLLKKLWSSGRNSVAPRAFKTKLARFAPQFSGYNQHDSQELLAFLLDGLHEDLNKVKR 439

Query: 498 KPYIKSRDADGRPDEEVADEYWANHIARNDSIIVDVCQGQYKSTLVCPVCNKVSVTFDPF 557
           KPYI+ +D+D RPD+EVA+E W  H ARNDS+IVDVCQGQYKSTLVCP C K+S+TFDPF
Sbjct: 440 KPYIELKDSDSRPDDEVAEELWNYHKARNDSVIVDVCQGQYKSTLVCPACGKISITFDPF 499

Query: 558 MYLSLPLQSTTTRTMTATLFTSDGSKLPSTLTVTVPKQGRCRDLIQALNSACLLKHSEKL 617
           MYLS+PL ST TR+MT T+F  DGS LP   TV VPK G  RDLI AL +ACLL   E L
Sbjct: 500 MYLSVPLPSTLTRSMTVTVFYCDGSHLPMPYTVIVPKNGSIRDLITALGTACLLAEDESL 559

Query: 618 LLAEVRDHLIHRFLEDPLISLSTIKDDDHIAAYKIPKLTKNT--KYLQLVHRRQEQVTSD 677
           LLAEV DH I ++ E+PL SLS+IKDD+HI AY++ ++ K +    L+++H  Q++   +
Sbjct: 560 LLAEVYDHKIFKYFENPLDSLSSIKDDEHIVAYRLNQMPKGSGKAKLEILHGGQKRPILE 619

Query: 678 AHAMSGWRPFGTPLVSVVSCDDPVTKGYIQRIIKRVLSPMAKTGTLMDTDVFDSSVSHAG 737
           +      + FGTP V+ V+  +P++   I  ++ R LSP+ K           S + +  
Sbjct: 620 SVRGRDVKLFGTPFVTYVN-TEPLSGADIDAVLSRFLSPLHKVHA-------PSKIHNGS 679

Query: 738 EDLHPRDSSIETCTVSISSLMNKDDPKSKGAEPFKLPLQLLNKDNVCIDLSTGEESVQLP 797
           E+ H  D++++  +  +SS   + D  S     F+  + L ++  +       E S+ L 
Sbjct: 680 ENGHLPDATVDEASEILSSPDTEIDDASDRELSFR--IFLTDERGLNFKPLQSESSISLG 739

Query: 798 PSSSVLVYIDWSQKLMKKFDTFYLENLPEVFKSGPVMKKARTEPLSLYSCLESFLREEPL 857
            ++ VLV  +W++   +++D+ YL +LPEV K+    KK R E +SL+SCLE+FL EEPL
Sbjct: 740 IATRVLV--EWNEGEHERYDSSYLSDLPEVHKTSFSAKKTRQESISLFSCLEAFLAEEPL 799

Query: 858 VPEDMWFCPQCKERRQASKKLDLWRLPEVLVIHLKRFSYSRSMKHKLETFVNFPIHDFDL 917
            P+DMWFCP CKE RQA+KKLDLW+LP++LV HLKRF+YSR +K+K++TFVNFP+HD DL
Sbjct: 800 GPDDMWFCPSCKEHRQANKKLDLWKLPDILVFHLKRFTYSRYLKNKIDTFVNFPVHDLDL 859

Query: 918 TNYVANKNNSQRQLYELYALTNHYGGMGSGHYTAHIKLIDENRWYNFDDSHISLINEEEV 977
           + YV NKN+ Q  LYELYA++NHYGG+G GHYTA+ KLID+N WY+FDDSH+S +NE E+
Sbjct: 860 SKYVKNKND-QSYLYELYAVSNHYGGLGGGHYTAYAKLIDDNEWYHFDDSHVSSVNESEI 897

Query: 978 KSAAAYVLFYRRVRTE 991
           K++AAYVLFYRRVR+E
Sbjct: 920 KNSAAYVLFYRRVRSE 897

BLAST of MC03g0310 vs. TAIR 10
Match: AT1G32850.1 (ubiquitin-specific protease 11 )

HSP 1 Score: 768.1 bits (1982), Expect = 8.9e-222
Identity = 431/935 (46.10%), Postives = 594/935 (63.53%), Query Frame = 0

Query: 66  VSMCSSGGRTELTPEEERIMIRDIALTAEANTKEGDVFYLITQRWWQHWIEYVNQDQPVN 125
           + +C S      TPEEER ++ ++   AEA+ KEG+++++I+ RW+  W  +V       
Sbjct: 10  IGVCES----SYTPEEERRIVTELNNEAEADLKEGNLYFVISNRWYTRWQRFV------- 69

Query: 126 ANDGSSFAEIYDSFGSSMLKRPANIDNSDLIYDAASEDSSVSIEIHDTLLEGRDYVLLPQ 185
                   E + S   S + RP  IDN D+I    SE  +   ++   L EG DY L+ Q
Sbjct: 70  ----GLLTEEFRSGEPSEVTRPGPIDNHDII---DSESDASDPQLRMMLEEGVDYTLVQQ 129

Query: 186 EVWKQLYLWYGGGPTLARKVISAGLSQTELAVEVYPLRLQLLEVGKGDRSTIRISKKETI 245
           EVW++L  WY GGP + RK+IS G      +VEVY L L L +      + IR+SK+ +I
Sbjct: 130 EVWRKLVKWYKGGPPVPRKLISQGFYTKSFSVEVYLLCLTLTDSRDESTTIIRLSKQASI 189

Query: 246 GELHRRACEIFDLNLEQVCIWDYYGHRKHALMN-DMDKTLDDANIQMDQDILVEIINHDN 305
           G+L+   C    +  E+  IWDY+  +K  L++   ++++++A +Q +QDIL+E+     
Sbjct: 190 GQLYEMVCAGKGVAKEKARIWDYFEKKKSVLLDPSSEQSVEEAGLQFNQDILLEV----- 249

Query: 306 NNALGGCTSYVQDNGTTDKEATSISLESSKS---NLIIAGGVSNKGAS---KTEVVQSQS 365
                G  S        + E   + LE  +S   +++  GG  + G S   K       +
Sbjct: 250 ----DGSASSQFVMSLAENELAMVPLEPMRSDAMDIVRGGGTLSNGHSNGFKFSFFGRNT 309

Query: 366 LTSSIRESDNTYGQSGVSTRGSSCGLTGLLNLGNTCFMNSAIQCLVHTPEFARYFREDYH 425
               +  S  T+G      +G   GL GL NLGNTCFMNS +QCL HTP    YF +DY 
Sbjct: 310 FKDDV--SSRTFG------KGEKRGLGGLQNLGNTCFMNSTLQCLAHTPPIVEYFLQDYR 369

Query: 426 QEINRQNPLGMVGELAMAFGDLLRKLWAPGRTSVAPRPFKAKLARFAPQFSGYNQHDSQE 485
            +IN +NPLGM GELA+AFG+LLRKLW+ G+ +VAPR FK KLARFAPQFSGYNQHDSQE
Sbjct: 370 SDINAKNPLGMRGELAIAFGELLRKLWSSGQNTVAPRAFKTKLARFAPQFSGYNQHDSQE 429

Query: 486 LLAFLLDGLHEDLNRVKHKPYIKSRDADGRPDEEVADEYWANHIARNDSIIVDVCQGQYK 545
           +LAFLLDGLHEDLN+VK KPYI+++D+DGRPD+EVA+E W  H ARNDS+IVDV QGQYK
Sbjct: 430 MLAFLLDGLHEDLNKVKRKPYIEAKDSDGRPDDEVAEEKWKYHKARNDSVIVDVFQGQYK 489

Query: 546 STLVCPVCNKVSVTFDPFMYLSLPLQSTTTRTMTATLFTSDGSKLPSTLTVTVPKQGRCR 605
           STLVCP C K+S+TFDPFMYLSLPL S+ TR+MT T+F  DGS LP   TVTVPK G CR
Sbjct: 490 STLVCPDCGKISITFDPFMYLSLPLPSSRTRSMTVTVFYGDGSHLPMPYTVTVPKDGSCR 549

Query: 606 DLIQALNSACLLKHSEKLLLAEVRDHLIHRFLEDPLISLSTIKDDDHIAAYKIPKLTK-- 665
           DL  AL +AC L + E LLLAEV DH + ++ E+P   L+ IKD++HI AY+  ++ K  
Sbjct: 550 DLSNALGTACCLDNDESLLLAEVYDHKVFKYYENPRELLNGIKDNEHIVAYRFKQMHKGP 609

Query: 666 NTKYLQLVHRRQEQVTSDAHAMSGWRPFGTPLVSVVSCDDPVTKGYIQRIIKRVLSPMAK 725
               L+++H  QE+ +SD     G + FGTPLV+ ++  +P++   I   I  +LSP+ +
Sbjct: 610 GKVKLEILHGEQEK-SSD----RGPKCFGTPLVTYIN-KEPLSGTDIATSISGLLSPLRR 669

Query: 726 TGTLMDTDVFDSSVSHAG-EDLHPRDSSIETCTVSISSLMNKDDPKSKGAEPFKLPLQLL 785
                   V  S V ++G E+ H  D S         S++++D          +L L LL
Sbjct: 670 --------VHMSCVVNSGNENGHVPDESSR-------SILSRDTETEDNDR--ELSLSLL 729

Query: 786 NKDNVCIDLSTGEESVQLPPSSSVLVYIDWSQKLMKKFDTFYLENLPEVFKSGPVMKKAR 845
            +D    +L   E    + P S   V + W++K  +K+D+ YL +LP+V K+  + KK  
Sbjct: 730 -RDYYSFNLQPLESDSVVNPGSVTKVLVKWNEKEHEKYDSSYLNDLPKVHKN-VLAKKTM 789

Query: 846 TEPLSLYSCLESFLREEPLVPEDMWFCPQCKERRQASKKLDLWRLPEVLVIHLKRFSYSR 905
            E +SL+SCLE+FL EEPL P+DMW+CP CKE RQA+KKLDLW+LP++LV HLKRF+YSR
Sbjct: 790 QEGISLFSCLEAFLAEEPLGPDDMWYCPGCKEHRQANKKLDLWKLPDILVFHLKRFTYSR 849

Query: 906 SMKHKLETFVNFPIHDFDLTNYVANKNNSQRQLYELYALTNHYGGMGSGHYTAHIKLIDE 965
             K+K++T VNF IHD DL+ YV N+ + Q  LYELYA++NHYGG+G GHYTA+ KL+DE
Sbjct: 850 YFKNKIDTLVNFHIHDLDLSKYVKNE-DGQSYLYELYAISNHYGGLGGGHYTAYAKLMDE 883

Query: 966 NRWYNFDDSHISLINEEEVKSAAAYVLFYRRVRTE 991
            +WYNFDDS +S +NE E+K++AAYVLFY+RV+++
Sbjct: 910 TKWYNFDDSRVSAVNESEIKTSAAYVLFYQRVKSD 883