MC03g0028 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC03g0028
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	RNA-directed DNA methylation 4
Location	MC03: 380666 .. 384299 (+)
RNA-Seq Expression	MC03g0028
Synteny	MC03g0028

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

CCCATTTGTGATGACTAATCAGGGTTTCTCGCCTATTCCAGCGGGGCAAGCGACATCAATCATCACTCTCTCTTCCTTTTCGATTTTTTCTTTTCAAAGCCAAAGCAATCCCTCACTACTCCCTCCTGTTCCTTGCCGCACGCCCCAGCGCCCGCAACCTTACCGACCACCGCTCGGCTCGCCTCTGTATCGCCGCCGCAGCCTCGCTTAGGCGTCTCTGCGATCTGTTCCGCCACCCCGTCGCACGCTGCACGCCGGTGGAATCCCCAACGCAAGAAGCCTCAGCGCTGTTGACATGGCTGAGAAGGACGAGGGAAAGCTGACTAATCCGACCGATAAAAGCCGAATCGGAGCGGACGAACTCGGTTCCTAATGTTCGCTTCGTTGCGGTCCAACCGAACCGACTGGGAACTGAAATTGTGTACTTGGTGGTCTCAAGACTTGCATGTCCCTGAGCATAAATCATTGTCTTCGATCGCCTCTCCTCTCGTATGGCTACCATCGGCGAGAGCTCTTCGTCTGTTCCCAAACTCGCTGACGGAAAGCCAGTGTTGGTTAGGGTTAAGCGCAAAGCTTCCCAATCTCGACTCGATGCACTATGTGAGTTGGGACACTCTTTCATGATCCTTTCTTTCGATTTTTTATTGTTTATGAACCATCAAACGAAGTTTGAATTGTTCGTGCCCGCGTTGACGTTTTGGTATGTTTGGATAATTTGTGTGCCGTAGGGCTGGAGATCAATGAGAGGCCACTCAAGCGACCTCTGCTGGATTTTGAGAACTTATCTATCTCAGAAACATTCCAGAATGGTCCGTCCAGCTCTGTACCTGTACTTGCGTTTTTTTTTTTCTGTTCCTGTCACTTGGTAATCTGCATAGGTTAAAAGAGGAAACATCAATTAGCAGCTATAGGTTTCTTCCAGACAAATATCCGAAAATTAGCTTCCCTAGTTTATACTATAGTATTTGAAGGGGGGGTGATTAAAAAAAAAACTAAATTCTTTGTTATATGGATTTTTTTTGTAGGAGAATCGTGTTAACCCTTTATAAGGAAAGTAATGGATGGATTGTATTGTTGTTATTGTTTCTTCCTTTTTGTGGAGGAGGGAAGATATGGGTTTCTTCACTTTATGGTTCTTGCCAGTTGATTAAATATCAATTTGAGATATCAACCTCGATTAAGCTTCTAAGATTTACTATAATTCACGATTACATAGCAAATTAAGTGTGATTTCACTCCCTGCCCCTGATGGAATAGCTTTTACTTGCATCAGAGGAACTTAAGACTAAGAAGATATTTGTACAGCACGTGGAGACATTGAAAAGCTCTGAGGCCACTGTTGACATTGTACAGTCCTTTGTGGTTAGTATCTTGGTCAGTTTAAGTCTTCTTTTTGACATTTTAACTAGCATCTTTGTCGGTAACTGTCTTATCTTTAGGCACCTGATGCTGCTGGCACCGTTGAGAATAGCGTAAAGAATGAGGAGCGTAGAAGAAATTTTAAGAGAGAGATTGTATGTCTTGTCTACTCGTTTGTTTGGTCCATTAGTTTGTGTGCTACTTATTTAATAGTTTGCTTACCTTGTCTAGTATTCTGCAGCCAAGACAAGATCAGCTGTTGAGTAAAGCTAGACAAGAACAAGAGGTATGGACCGTATGGTATATGATGACATTCCTTTAATGCTTTTTAAACGATAAATTCAATCATTGTTTTATCCGAAGAGTTTTGGTCATATTAGATTATAATGTGTTTATAGTTTGTTTAAACTGTTTTCATATGTTGTACTGTTACTTTTTGTTGGTAACATTCTTTCTTTGTGCTACTGCTTTCTCTTTCTATCAGGTTTTAGCAAAAAATGCTCGATTTGAGCAGATATGGAGAAGTAGGAAAGGGGTTAAAGATGCAAAAGATGACCAATTACATGACATGTATCATATCTATGATATTGTTCGTCTTGATACAAACAAAATATCAAGTGAAGTACCGGAGCAGGAGTGAGTTCTGTCACGATGATACTATTGGATCTTGATTTTCTATAAACTCCTCATTTATGTGGACAAACGTGCTCTTGCTATTAATGCAGGTATATGTCCCTGGAGGATCAGAGTATGTTATCGAGTTACCTGCCTTTACTAAGGGAGTTTATCCCAAGTGCTGCTGCAGAAATTGAGTCAGATATACATGCAAACATGATGAAACAAGATGGTTGGTTTTTTTTAATCTAGCAGTCACTTGAGGCATTTTGCTTACGGATGTAGTGGTAAATAAATAATGGAAGTAAGGTTCTAGGCAATTCAGCAGTAGATGTGTGTTGGATGAGGATAATTATCGTCCATCTTTTATTGGTTGTTCCAGCTATTGTCTCCAGTCCTTGCTATGCCTAAGTTCCTTCCTAGTTCACCCTTTTGTTAATATTATTTGCATGCTTGTATGACTGTTTTCGTTACTCGTTTATGTAACTTCTTACTGTTATTTCGGTGTCTTCACTTGTTCTACAGTGCCTGTAGATGATTATGTATATGACTACTACACTGTGAAGAGTGACATGGAGATTGCTGAAGACGATGCCTCTAATCCATTTCCTTTGTGCGTCATTGTCTCTCTCTGTTTTCATTTCGTTTGTTTCCTCTCTGTGTTCCCTTTTTGCAATAAATTCACATCTTTTTCCAGGATACAAGTTGATGATTTGGATCTATATGATGGGCCTGATGACTCAGATTACGAAAGCGATGATTCAAATGGTAAATATCTTTTTCCTTGATCCTTGTAGTTTAGAAACCAAAGTTACATCTGATTCTTTTTTGCAAATATCTTTTTCGGTTTCAGCTGAAAACAATCCACACTTTGATTACCCTGATGAGGTATCGGAAGAAGACGACGAGTTGGGGAGTGAATCTTCAAATGGTGAATCAAAAGATAATGATGATGACAGCGAGAAGAAGCAGTCGTCGGAAGCTAATGACTCAGAAGAGGATGACCTGTCAGTGGACAGAGCCCAATTATACGAGGATGAAATATATGGTGATTTTGATGATGATGATGCTAATAGTTTCGATTGTGACAGTAATGGTGGTCATGATGAGGGTGAGGATTCGAGATGGTCCTATCGTTGAATTGAATGTTTGTTCCACCGCATTCCCTATCAGCTCAGCTAGTACTATTTTTTCCAGTGAGATGAGATGCTTACTTGTGAGGAAGGCGTTTGTAGGTAGTAATGATAGGGATTTGCCATTTGAGAAGTTTGCTATCTGATCTTCATGAGTAGATATTGAAAGCCAGCCTATGAGGTCATGGTTATATAAAATGGAATGAGTTCCATTCTGATGACCTCTGGAAGGGTGATTTAAAGAACTTCTCAGAATTCCTAAGGGGTGGTTGTTTATGCAATTTTGATTCTATTTCTAGATATCTGTTTGGCAAATTTTTTTCGAATTTTTTTTTTGAAATCTGTGATTTAAAAAGTAGACATTTTTGTATTTTTAGGTTTTGAATTTGGAGTAGAATTACACTAAAAAAATGTATTATAAATTATTTAAATATTGTAAAATAATAATTCTATAAAATTTATAATATATATTTATTAAATTAATTAAACATATTTAATAACTACCACTAATTACTAATAATTTTTTA

mRNA sequence

CCCATTTGTGATGACTAATCAGGGTTTCTCGCCTATTCCAGCGGGGCAAGCGACATCAATCATCACTCTCTCTTCCTTTTCGATTTTTTCTTTTCAAAGCCAAAGCAATCCCTCACTACTCCCTCCTGTTCCTTGCCGCACGCCCCAGCGCCCGCAACCTTACCGACCACCGCTCGGCTCGCCTCTGTATCGCCGCCGCAGCCTCGCTTAGGCGTCTCTGCGATCTGTTCCGCCACCCCGTCGCACGCTGCACGCCGGTGGAATCCCCAACGCAAGAAGCCTCAGCGCTGTTGACATGGCTGAGAAGGACGAGGGAAAGCTGACTAATCCGACCGATAAAAGCCGAATCGGAGCGGACGAACTCGGTTCCTAATGTTCGCTTCGTTGCGGTCCAACCGAACCGACTGGGAACTGAAATTGTGTACTTGGTGGTCTCAAGACTTGCATGTCCCTGAGCATAAATCATTGTCTTCGATCGCCTCTCCTCTCGTATGGCTACCATCGGCGAGAGCTCTTCGTCTGTTCCCAAACTCGCTGACGGAAAGCCAGTGTTGGTTAGGGTTAAGCGCAAAGCTTCCCAATCTCGACTCGATGCACTATGGCTGGAGATCAATGAGAGGCCACTCAAGCGACCTCTGCTGGATTTTGAGAACTTATCTATCTCAGAAACATTCCAGAATGAGGAACTTAAGACTAAGAAGATATTTGTACAGCACGTGGAGACATTGAAAAGCTCTGAGGCCACTGTTGACATTGTACAGTCCTTTGTGGCACCTGATGCTGCTGGCACCGTTGAGAATAGCGTAAAGAATGAGGAGCGTAGAAGAAATTTTAAGAGAGAGATTCCAAGACAAGATCAGCTGTTGAGTAAAGCTAGACAAGAACAAGAGGTTTTAGCAAAAAATGCTCGATTTGAGCAGATATGGAGAAGTAGGAAAGGGGTTAAAGATGCAAAAGATGACCAATTACATGACATGTATCATATCTATGATATTGTTCGTCTTGATACAAACAAAATATCAAGTGAAGTACCGGAGCAGGAGTATATGTCCCTGGAGGATCAGAGTATGTTATCGAGTTACCTGCCTTTACTAAGGGAGTTTATCCCAAGTGCTGCTGCAGAAATTGAGTCAGATATACATGCAAACATGATGAAACAAGATGTGCCTGTAGATGATTATGTATATGACTACTACACTGTGAAGAGTGACATGGAGATTGCTGAAGACGATGCCTCTAATCCATTTCCTTTGATACAAGTTGATGATTTGGATCTATATGATGGGCCTGATGACTCAGATTACGAAAGCGATGATTCAAATGCTGAAAACAATCCACACTTTGATTACCCTGATGAGGTATCGGAAGAAGACGACGAGTTGGGGAGTGAATCTTCAAATGGTGAATCAAAAGATAATGATGATGACAGCGAGAAGAAGCAGTCGTCGGAAGCTAATGACTCAGAAGAGGATGACCTGTCAGTGGACAGAGCCCAATTATACGAGGATGAAATATATGGTGATTTTGATGATGATGATGCTAATAGTTTCGATTGTGACAGTAATGGTGGTCATGATGAGGGTGAGGATTCGAGATGGTCCTATCGTTGAATTGAATGTTTGTTCCACCGCATTCCCTATCAGCTCAGCTAGTACTATTTTTTCCAGTGAGATGAGATGCTTACTTGTGAGGAAGGCGTTTGTAGGTAGTAATGATAGGGATTTGCCATTTGAGAAGTTTGCTATCTGATCTTCATGAGTAGATATTGAAAGCCAGCCTATGAGGTCATGGTTATATAAAATGGAATGAGTTCCATTCTGATGACCTCTGGAAGGGTGATTTAAAGAACTTCTCAGAATTCCTAAGGGGTGGTTGTTTATGCAATTTTGATTCTATTTCTAGATATCTGTTTGGCAAATTTTTTTCGAATTTTTTTTTTGAAATCTGTGATTTAAAAAGTAGACATTTTTGTATTTTTAGGTTTTGAATTTGGAGTAGAATTACACTAAAAAAATGTATTATAAATTATTTAAATATTGTAAAATAATAATTCTATAAAATTTATAATATATATTTATTAAATTAATTAAACATATTTAATAACTACCACTAATTACTAATAATTTTTTA

Coding sequence (CDS)

ATGGCTACCATCGGCGAGAGCTCTTCGTCTGTTCCCAAACTCGCTGACGGAAAGCCAGTGTTGGTTAGGGTTAAGCGCAAAGCTTCCCAATCTCGACTCGATGCACTATGGCTGGAGATCAATGAGAGGCCACTCAAGCGACCTCTGCTGGATTTTGAGAACTTATCTATCTCAGAAACATTCCAGAATGAGGAACTTAAGACTAAGAAGATATTTGTACAGCACGTGGAGACATTGAAAAGCTCTGAGGCCACTGTTGACATTGTACAGTCCTTTGTGGCACCTGATGCTGCTGGCACCGTTGAGAATAGCGTAAAGAATGAGGAGCGTAGAAGAAATTTTAAGAGAGAGATTCCAAGACAAGATCAGCTGTTGAGTAAAGCTAGACAAGAACAAGAGGTTTTAGCAAAAAATGCTCGATTTGAGCAGATATGGAGAAGTAGGAAAGGGGTTAAAGATGCAAAAGATGACCAATTACATGACATGTATCATATCTATGATATTGTTCGTCTTGATACAAACAAAATATCAAGTGAAGTACCGGAGCAGGAGTATATGTCCCTGGAGGATCAGAGTATGTTATCGAGTTACCTGCCTTTACTAAGGGAGTTTATCCCAAGTGCTGCTGCAGAAATTGAGTCAGATATACATGCAAACATGATGAAACAAGATGTGCCTGTAGATGATTATGTATATGACTACTACACTGTGAAGAGTGACATGGAGATTGCTGAAGACGATGCCTCTAATCCATTTCCTTTGATACAAGTTGATGATTTGGATCTATATGATGGGCCTGATGACTCAGATTACGAAAGCGATGATTCAAATGCTGAAAACAATCCACACTTTGATTACCCTGATGAGGTATCGGAAGAAGACGACGAGTTGGGGAGTGAATCTTCAAATGGTGAATCAAAAGATAATGATGATGACAGCGAGAAGAAGCAGTCGTCGGAAGCTAATGACTCAGAAGAGGATGACCTGTCAGTGGACAGAGCCCAATTATACGAGGATGAAATATATGGTGATTTTGATGATGATGATGCTAATAGTTTCGATTGTGACAGTAATGGTGGTCATGATGAGGGTGAGGATTCGAGATGGTCCTATCGTTGA

Protein sequence

MATIGESSSSVPKLADGKPVLVRVKRKASQSRLDALWLEINERPLKRPLLDFENLSISETFQNEELKTKKIFVQHVETLKSSEATVDIVQSFVAPDAAGTVENSVKNEERRRNFKREIPRQDQLLSKARQEQEVLAKNARFEQIWRSRKGVKDAKDDQLHDMYHIYDIVRLDTNKISSEVPEQEYMSLEDQSMLSSYLPLLREFIPSAAAEIESDIHANMMKQDVPVDDYVYDYYTVKSDMEIAEDDASNPFPLIQVDDLDLYDGPDDSDYESDDSNAENNPHFDYPDEVSEEDDELGSESSNGESKDNDDDSEKKQSSEANDSEEDDLSVDRAQLYEDEIYGDFDDDDANSFDCDSNGGHDEGEDSRWSYR

Homology

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BLAST of MC03g0028 vs. ExPASy Swiss-Prot
Match: Q8GYP3 (RNA-directed DNA methylation 4 OS=Arabidopsis thaliana OX=3702 GN=RDM4 PE=1 SV=1)

HSP 1 Score: 250.4 bits (638), Expect = 3.3e-65
Identity = 162/361 (44.88%), Postives = 231/361 (63.99%), Query Frame = 0


Query: 5   GESSSSVPKLADGKPVLVRVKRKASQSRLDALWLEINERPLKRPLLDFENLSISETFQN- 64
           G   SS     + KPV+VRVKRK  QS LDA WLEINERPLKRP LDF  LSIS++ +  
Sbjct: 3   GVGESSTQNEVEEKPVIVRVKRKVGQSLLDAFWLEINERPLKRPTLDFSKLSISDSGERG 62

Query: 65  ----EELKTKKIFVQHVETLKSSEATVDIVQSFVAPDAAGTVENSVKNEERRRNFKREIP 124
               E++K KK+ V+H+ET+  SE T DI+ SF   D      +  K EER+  FK++  
Sbjct: 63  PSVAEDVKPKKVLVRHLETVTDSETTADIIHSFFESDHNEKSCSKGKFEERKIAFKKD-N 122

Query: 125 RQDQLLSKARQEQEVLAKNARFEQIWRSRKGVKDAKDDQLHDMYHIYDIVRLDTNKISSE 184
           R++Q L+K+ Q+Q++ ++NARFEQIWRSRKG K+     +H+  H +D++R+DT +    
Sbjct: 123 RKEQRLTKSVQKQQIASENARFEQIWRSRKGNKEG----IHEKCHFFDVIRVDTEERRDN 182

Query: 185 VPEQEYMSLEDQSMLSSYLPLLREFIPSAAAEIESDIHANMMKQDVPVDDYVYDYYTVKS 244
              QE+ SLEDQ ML+S+LPLLRE IP+AA EIE+DI      Q    ++YVYDYY V  
Sbjct: 183 A--QEFTSLEDQKMLASFLPLLRECIPTAAEEIEADI------QSSHTEEYVYDYYAVNE 242

Query: 245 DMEIAEDDASNPFPLIQVDDLDLY-DGPDDSDYESDDSNAENNPHFDYPDEVSEEDDELG 304
           +M+I+ED + N FPL+ V+D + + DG D+SDY+S+DSNAE++P  DYP+E  EE++E  
Sbjct: 243 EMDISEDSSKNQFPLVIVEDEEEFCDGSDESDYDSEDSNAEDHPKTDYPEEEEEEEEE-- 302

Query: 305 SESSNGESKDNDDDSEKKQSSEANDSEEDD----------LSVDRAQLYEDEIYGDFDDD 350
                 +  D+DDD  +++ SEA+D  +D+          L  D    Y +++YG  + D
Sbjct: 303 ------DDDDDDDDESEEEKSEASDESDDEETSKRHVRSVLGDDEFDDYAEDVYGYSESD 342

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BLAST of MC03g0028 vs. NCBI nr
Match: XP_022154907.1 (RNA-directed DNA methylation 4 [Momordica charantia])

HSP 1 Score: 698 bits (1801), Expect = 1.12e-252
Identity = 372/372 (100.00%), Postives = 372/372 (100.00%), Query Frame = 0


Query: 1   MATIGESSSSVPKLADGKPVLVRVKRKASQSRLDALWLEINERPLKRPLLDFENLSISET 60
           MATIGESSSSVPKLADGKPVLVRVKRKASQSRLDALWLEINERPLKRPLLDFENLSISET
Sbjct: 1   MATIGESSSSVPKLADGKPVLVRVKRKASQSRLDALWLEINERPLKRPLLDFENLSISET 60

Query: 61  FQNEELKTKKIFVQHVETLKSSEATVDIVQSFVAPDAAGTVENSVKNEERRRNFKREIPR 120
           FQNEELKTKKIFVQHVETLKSSEATVDIVQSFVAPDAAGTVENSVKNEERRRNFKREIPR
Sbjct: 61  FQNEELKTKKIFVQHVETLKSSEATVDIVQSFVAPDAAGTVENSVKNEERRRNFKREIPR 120

Query: 121 QDQLLSKARQEQEVLAKNARFEQIWRSRKGVKDAKDDQLHDMYHIYDIVRLDTNKISSEV 180
           QDQLLSKARQEQEVLAKNARFEQIWRSRKGVKDAKDDQLHDMYHIYDIVRLDTNKISSEV
Sbjct: 121 QDQLLSKARQEQEVLAKNARFEQIWRSRKGVKDAKDDQLHDMYHIYDIVRLDTNKISSEV 180

Query: 181 PEQEYMSLEDQSMLSSYLPLLREFIPSAAAEIESDIHANMMKQDVPVDDYVYDYYTVKSD 240
           PEQEYMSLEDQSMLSSYLPLLREFIPSAAAEIESDIHANMMKQDVPVDDYVYDYYTVKSD
Sbjct: 181 PEQEYMSLEDQSMLSSYLPLLREFIPSAAAEIESDIHANMMKQDVPVDDYVYDYYTVKSD 240

Query: 241 MEIAEDDASNPFPLIQVDDLDLYDGPDDSDYESDDSNAENNPHFDYPDEVSEEDDELGSE 300
           MEIAEDDASNPFPLIQVDDLDLYDGPDDSDYESDDSNAENNPHFDYPDEVSEEDDELGSE
Sbjct: 241 MEIAEDDASNPFPLIQVDDLDLYDGPDDSDYESDDSNAENNPHFDYPDEVSEEDDELGSE 300

Query: 301 SSNGESKDNDDDSEKKQSSEANDSEEDDLSVDRAQLYEDEIYGDFDDDDANSFDCDSNGG 360
           SSNGESKDNDDDSEKKQSSEANDSEEDDLSVDRAQLYEDEIYGDFDDDDANSFDCDSNGG
Sbjct: 301 SSNGESKDNDDDSEKKQSSEANDSEEDDLSVDRAQLYEDEIYGDFDDDDANSFDCDSNGG 360

Query: 361 HDEGEDSRWSYR 372
           HDEGEDSRWSYR
Sbjct: 361 HDEGEDSRWSYR 372

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BLAST of MC03g0028 vs. NCBI nr
Match: XP_023522069.1 (RNA-directed DNA methylation 4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 572 bits (1473), Expect = 8.87e-203
Identity = 308/372 (82.80%), Postives = 339/372 (91.13%), Query Frame = 0


Query: 1   MATIGESSSSVPKLADGKPVLVRVKRKASQSRLDALWLEINERPLKRPLLDFENLSISET 60
           MA+IGESSSS PK AD KPVLVRVKRKASQSRLDALWLEINERPLKRPLLDF+NLSISET
Sbjct: 1   MASIGESSSSAPKFADEKPVLVRVKRKASQSRLDALWLEINERPLKRPLLDFDNLSISET 60

Query: 61  FQNEELKTKKIFVQHVETLKSSEATVDIVQSFVAPDAAGTVENSVKNEERRRNFKREIPR 120
           FQ EELKTKKIFVQHVETL+SSEA+VDI+QSFVAPDAAG +EN++KNEERRRNFKRE PR
Sbjct: 61  FQKEELKTKKIFVQHVETLRSSEASVDIIQSFVAPDAAGNIENNLKNEERRRNFKRESPR 120

Query: 121 QDQLLSKARQEQEVLAKNARFEQIWRSRKGVKDAKDDQLHDMYHIYDIVRLDTNKISSEV 180
           Q+QLL K RQEQEVLAK ARFEQIWRSRKG+KD KDDQLHD YHIYDIVRLDTN+ SSE 
Sbjct: 121 QEQLLVKTRQEQEVLAKKARFEQIWRSRKGIKDPKDDQLHDAYHIYDIVRLDTNETSSEA 180

Query: 181 PEQEYMSLEDQSMLSSYLPLLREFIPSAAAEIESDIHANMMKQDVPVDDYVYDYYTVKSD 240
           P+ E M+LEDQSMLSSYLPLLREFIPSAA EIESDI+AN +KQD+PVDDYVYDYYTVK++
Sbjct: 181 PKAECMTLEDQSMLSSYLPLLREFIPSAAEEIESDINANTLKQDLPVDDYVYDYYTVKNN 240

Query: 241 MEIAEDDASNPFPLIQVDDLDLYDGPDDSDYESDDSNAENNPHFDYPDEVSEEDDELGSE 300
           + IA+DDASNPFPLIQVDDLDLYDGPDDS+YESDDSNAENNPHFDYPDE SEE+ EL SE
Sbjct: 241 VPIADDDASNPFPLIQVDDLDLYDGPDDSEYESDDSNAENNPHFDYPDEASEEE-ELESE 300

Query: 301 SSNGESKDNDDDSEKKQSSEANDSEEDDLSVDRAQLYEDEIYGDFDDDDANSFDCDSNGG 360
           S+N ES+++D+D   KQSSEAND EEDDLS +  QL EDEIYGDFD+DD +SFD DSNGG
Sbjct: 301 STNEESEEDDED---KQSSEANDLEEDDLSENGVQLDEDEIYGDFDEDDGDSFDYDSNGG 360

Query: 361 HDEGEDSRWSYR 372
           H+EGED RWSYR
Sbjct: 361 HEEGEDWRWSYR 368

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BLAST of MC03g0028 vs. NCBI nr
Match: KAG6607356.1 (RNA-directed DNA methylation 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 572 bits (1473), Expect = 8.87e-203
Identity = 309/372 (83.06%), Postives = 338/372 (90.86%), Query Frame = 0


Query: 1   MATIGESSSSVPKLADGKPVLVRVKRKASQSRLDALWLEINERPLKRPLLDFENLSISET 60
           MA+IGESSSS PK AD KPVLVRVKRKASQSRLDALWLEINERPLKRPLLDF+NLSISET
Sbjct: 1   MASIGESSSSAPKFADEKPVLVRVKRKASQSRLDALWLEINERPLKRPLLDFDNLSISET 60

Query: 61  FQNEELKTKKIFVQHVETLKSSEATVDIVQSFVAPDAAGTVENSVKNEERRRNFKREIPR 120
           FQ EELKTKKIFVQHVETL+SSEA+VDI+QSFVAPDAAG +EN++KNEERRRNFKRE PR
Sbjct: 61  FQKEELKTKKIFVQHVETLRSSEASVDIIQSFVAPDAAGNIENNLKNEERRRNFKRESPR 120

Query: 121 QDQLLSKARQEQEVLAKNARFEQIWRSRKGVKDAKDDQLHDMYHIYDIVRLDTNKISSEV 180
           Q+QLL K RQEQEVLAK ARFEQIWRSRKG+KD KDDQLHD YHIYDIVRLDTN+ SSE 
Sbjct: 121 QEQLLVKTRQEQEVLAKKARFEQIWRSRKGIKDPKDDQLHDAYHIYDIVRLDTNETSSEA 180

Query: 181 PEQEYMSLEDQSMLSSYLPLLREFIPSAAAEIESDIHANMMKQDVPVDDYVYDYYTVKSD 240
           P+ E M+LEDQSMLSSYLPLLREFIPSAA EIESDI+AN MKQD+PVDDYVYDYYTVK++
Sbjct: 181 PKAECMTLEDQSMLSSYLPLLREFIPSAAEEIESDINANTMKQDLPVDDYVYDYYTVKNN 240

Query: 241 MEIAEDDASNPFPLIQVDDLDLYDGPDDSDYESDDSNAENNPHFDYPDEVSEEDDELGSE 300
           + IAEDDASNPFPLIQVDDLDLYDGPDDSDYESDDSNAENNPHFDYPDE SEE+ EL SE
Sbjct: 241 VPIAEDDASNPFPLIQVDDLDLYDGPDDSDYESDDSNAENNPHFDYPDEASEEE-ELESE 300

Query: 301 SSNGESKDNDDDSEKKQSSEANDSEEDDLSVDRAQLYEDEIYGDFDDDDANSFDCDSNGG 360
           S+N ES+++DDD   KQSSEAND EED+LS +  QL +DE+YGDFD+DD +SFD DSNGG
Sbjct: 301 STNEESEEDDDD---KQSSEANDLEEDNLSENGVQLDDDEVYGDFDEDDDDSFDYDSNGG 360

Query: 361 HDEGEDSRWSYR 372
           H EGED RWSYR
Sbjct: 361 HGEGEDWRWSYR 368

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BLAST of MC03g0028 vs. NCBI nr
Match: XP_022949593.1 (RNA-directed DNA methylation 4 [Cucurbita moschata])

HSP 1 Score: 568 bits (1465), Expect = 1.46e-201
Identity = 308/372 (82.80%), Postives = 337/372 (90.59%), Query Frame = 0


Query: 1   MATIGESSSSVPKLADGKPVLVRVKRKASQSRLDALWLEINERPLKRPLLDFENLSISET 60
           MA+IGESSSS PK AD KPVLVRVKRKASQSRLDALWLEINERPLKRPLLDF+NLSISET
Sbjct: 1   MASIGESSSSAPKFADEKPVLVRVKRKASQSRLDALWLEINERPLKRPLLDFDNLSISET 60

Query: 61  FQNEELKTKKIFVQHVETLKSSEATVDIVQSFVAPDAAGTVENSVKNEERRRNFKREIPR 120
           FQ EELKTKKIFVQHVETL+SSEA+VDI+QSFVAPDAAG +EN++KNEERRRNFKRE PR
Sbjct: 61  FQKEELKTKKIFVQHVETLRSSEASVDIIQSFVAPDAAGNIENNLKNEERRRNFKRESPR 120

Query: 121 QDQLLSKARQEQEVLAKNARFEQIWRSRKGVKDAKDDQLHDMYHIYDIVRLDTNKISSEV 180
           Q+QLL K RQEQEVLAK ARFEQIWRSRKG+KD +DDQLHD YHIYDIVRLDTN+ SSE 
Sbjct: 121 QEQLLVKTRQEQEVLAKKARFEQIWRSRKGIKDPRDDQLHDAYHIYDIVRLDTNETSSEA 180

Query: 181 PEQEYMSLEDQSMLSSYLPLLREFIPSAAAEIESDIHANMMKQDVPVDDYVYDYYTVKSD 240
           P+ E M+LEDQSMLSSYLPLLREFIPSAA EIESDI+AN MKQD+PVDDYVYDYYTVK++
Sbjct: 181 PKAECMTLEDQSMLSSYLPLLREFIPSAAEEIESDINANTMKQDLPVDDYVYDYYTVKNN 240

Query: 241 MEIAEDDASNPFPLIQVDDLDLYDGPDDSDYESDDSNAENNPHFDYPDEVSEEDDELGSE 300
           + IAEDDASNPFPLIQVDDLDLYDGPDDSDYESDDSNAENNPHFDYPDE SEE+ EL SE
Sbjct: 241 VPIAEDDASNPFPLIQVDDLDLYDGPDDSDYESDDSNAENNPHFDYPDEASEEE-ELESE 300

Query: 301 SSNGESKDNDDDSEKKQSSEANDSEEDDLSVDRAQLYEDEIYGDFDDDDANSFDCDSNGG 360
           S+N ES+++D+D   KQSSEAN  EEDDLS +  QL EDEIYGDFD+DD +SFD DSNGG
Sbjct: 301 STNEESEEDDED---KQSSEANGLEEDDLSENGVQLDEDEIYGDFDEDDGDSFDYDSNGG 360

Query: 361 HDEGEDSRWSYR 372
            +EGED RWSYR
Sbjct: 361 QEEGEDWRWSYR 368

[Close]

BLAST of MC03g0028 vs. NCBI nr
Match: XP_038885561.1 (RNA-directed DNA methylation 4 [Benincasa hispida] >XP_038885562.1 RNA-directed DNA methylation 4 [Benincasa hispida])

HSP 1 Score: 565 bits (1456), Expect = 3.56e-200
Identity = 311/373 (83.38%), Postives = 337/373 (90.35%), Query Frame = 0


Query: 1   MATIGESSSSVPKLADGKPVLVRVKRKASQSRLDALWLEINERPLKRPLLDFENLSISET 60
           MA+IGESSSSVPKL D KPVLVRVKRKASQSRLDALWLEINERP KRPLLDFENLSISET
Sbjct: 1   MASIGESSSSVPKLVDEKPVLVRVKRKASQSRLDALWLEINERPSKRPLLDFENLSISET 60

Query: 61  FQNEELKTKKIFVQHVETLKSSEATVDIVQSFVAPDAAGTVENSVKNEERRRNFKREIPR 120
           F  EELKTKKIFVQHVETL+SSEATVDIVQSF APDAA T+EN++KNEERRR FK+EI R
Sbjct: 61  FHKEELKTKKIFVQHVETLRSSEATVDIVQSFGAPDAASTIENNLKNEERRRKFKKEISR 120

Query: 121 QDQLLSKARQEQEVLAKNARFEQIWRSRKGVKDAKDDQLHDMYHIYDIVRLDTNKISSEV 180
           QDQLL KARQEQEVLAKNARFEQIWRSRKGVKD+KDDQLHD+YHIYDIVRLD N+IS E 
Sbjct: 121 QDQLLVKARQEQEVLAKNARFEQIWRSRKGVKDSKDDQLHDIYHIYDIVRLDANEISIEA 180

Query: 181 PEQE-YMSLEDQSMLSSYLPLLREFIPSAAAEIESDIHANMMKQDVPVDDYVYDYYTVKS 240
           P+QE  MSL+DQSMLSSYLPLLREFIPSAAAEIESDI ANMMKQD+P+DDYVYDYYTVK 
Sbjct: 181 PKQEEQMSLDDQSMLSSYLPLLREFIPSAAAEIESDIDANMMKQDLPIDDYVYDYYTVKD 240

Query: 241 DMEIAEDDASNPFPLIQVDDLDLYDGPDDSDYESDDSNAENNPHFDYPDEVSEEDDELGS 300
           ++EIA+DDASNPFPLIQVDDLDLYDGPDDSDYESDDSNAENNP FDYPDEVS+E+ EL S
Sbjct: 241 NVEIADDDASNPFPLIQVDDLDLYDGPDDSDYESDDSNAENNPCFDYPDEVSDEE-ELES 300

Query: 301 ESSNGESKDNDDDSEKKQSSEANDSEEDDLSVDRAQLYEDEIYGDFDDDDANSFDCDSNG 360
            S N E +DNDDD   KQSSE+N+ E+     D+ QLYEDEIYGDFD+DDA+SFD D+NG
Sbjct: 301 GSLNDELEDNDDD---KQSSESNELEDVFSEEDKIQLYEDEIYGDFDEDDADSFDYDTNG 360

Query: 361 GHDEGEDSRWSYR 372
           GHDEGED RWSYR
Sbjct: 361 GHDEGEDWRWSYR 369

[Close]

BLAST of MC03g0028 vs. ExPASy TrEMBL
Match: A0A6J1DMY7 (RNA-directed DNA methylation 4 OS=Momordica charantia OX=3673 GN=LOC111022052 PE=3 SV=1)

HSP 1 Score: 698 bits (1801), Expect = 5.44e-253
Identity = 372/372 (100.00%), Postives = 372/372 (100.00%), Query Frame = 0


Query: 1   MATIGESSSSVPKLADGKPVLVRVKRKASQSRLDALWLEINERPLKRPLLDFENLSISET 60
           MATIGESSSSVPKLADGKPVLVRVKRKASQSRLDALWLEINERPLKRPLLDFENLSISET
Sbjct: 1   MATIGESSSSVPKLADGKPVLVRVKRKASQSRLDALWLEINERPLKRPLLDFENLSISET 60

Query: 61  FQNEELKTKKIFVQHVETLKSSEATVDIVQSFVAPDAAGTVENSVKNEERRRNFKREIPR 120
           FQNEELKTKKIFVQHVETLKSSEATVDIVQSFVAPDAAGTVENSVKNEERRRNFKREIPR
Sbjct: 61  FQNEELKTKKIFVQHVETLKSSEATVDIVQSFVAPDAAGTVENSVKNEERRRNFKREIPR 120

Query: 121 QDQLLSKARQEQEVLAKNARFEQIWRSRKGVKDAKDDQLHDMYHIYDIVRLDTNKISSEV 180
           QDQLLSKARQEQEVLAKNARFEQIWRSRKGVKDAKDDQLHDMYHIYDIVRLDTNKISSEV
Sbjct: 121 QDQLLSKARQEQEVLAKNARFEQIWRSRKGVKDAKDDQLHDMYHIYDIVRLDTNKISSEV 180

Query: 181 PEQEYMSLEDQSMLSSYLPLLREFIPSAAAEIESDIHANMMKQDVPVDDYVYDYYTVKSD 240
           PEQEYMSLEDQSMLSSYLPLLREFIPSAAAEIESDIHANMMKQDVPVDDYVYDYYTVKSD
Sbjct: 181 PEQEYMSLEDQSMLSSYLPLLREFIPSAAAEIESDIHANMMKQDVPVDDYVYDYYTVKSD 240

Query: 241 MEIAEDDASNPFPLIQVDDLDLYDGPDDSDYESDDSNAENNPHFDYPDEVSEEDDELGSE 300
           MEIAEDDASNPFPLIQVDDLDLYDGPDDSDYESDDSNAENNPHFDYPDEVSEEDDELGSE
Sbjct: 241 MEIAEDDASNPFPLIQVDDLDLYDGPDDSDYESDDSNAENNPHFDYPDEVSEEDDELGSE 300

Query: 301 SSNGESKDNDDDSEKKQSSEANDSEEDDLSVDRAQLYEDEIYGDFDDDDANSFDCDSNGG 360
           SSNGESKDNDDDSEKKQSSEANDSEEDDLSVDRAQLYEDEIYGDFDDDDANSFDCDSNGG
Sbjct: 301 SSNGESKDNDDDSEKKQSSEANDSEEDDLSVDRAQLYEDEIYGDFDDDDANSFDCDSNGG 360

Query: 361 HDEGEDSRWSYR 372
           HDEGEDSRWSYR
Sbjct: 361 HDEGEDSRWSYR 372

[Close]

BLAST of MC03g0028 vs. ExPASy TrEMBL
Match: A0A6J1GCH8 (RNA-directed DNA methylation 4 OS=Cucurbita moschata OX=3662 GN=LOC111452902 PE=3 SV=1)

HSP 1 Score: 568 bits (1465), Expect = 7.09e-202
Identity = 308/372 (82.80%), Postives = 337/372 (90.59%), Query Frame = 0


Query: 1   MATIGESSSSVPKLADGKPVLVRVKRKASQSRLDALWLEINERPLKRPLLDFENLSISET 60
           MA+IGESSSS PK AD KPVLVRVKRKASQSRLDALWLEINERPLKRPLLDF+NLSISET
Sbjct: 1   MASIGESSSSAPKFADEKPVLVRVKRKASQSRLDALWLEINERPLKRPLLDFDNLSISET 60

Query: 61  FQNEELKTKKIFVQHVETLKSSEATVDIVQSFVAPDAAGTVENSVKNEERRRNFKREIPR 120
           FQ EELKTKKIFVQHVETL+SSEA+VDI+QSFVAPDAAG +EN++KNEERRRNFKRE PR
Sbjct: 61  FQKEELKTKKIFVQHVETLRSSEASVDIIQSFVAPDAAGNIENNLKNEERRRNFKRESPR 120

Query: 121 QDQLLSKARQEQEVLAKNARFEQIWRSRKGVKDAKDDQLHDMYHIYDIVRLDTNKISSEV 180
           Q+QLL K RQEQEVLAK ARFEQIWRSRKG+KD +DDQLHD YHIYDIVRLDTN+ SSE 
Sbjct: 121 QEQLLVKTRQEQEVLAKKARFEQIWRSRKGIKDPRDDQLHDAYHIYDIVRLDTNETSSEA 180

Query: 181 PEQEYMSLEDQSMLSSYLPLLREFIPSAAAEIESDIHANMMKQDVPVDDYVYDYYTVKSD 240
           P+ E M+LEDQSMLSSYLPLLREFIPSAA EIESDI+AN MKQD+PVDDYVYDYYTVK++
Sbjct: 181 PKAECMTLEDQSMLSSYLPLLREFIPSAAEEIESDINANTMKQDLPVDDYVYDYYTVKNN 240

Query: 241 MEIAEDDASNPFPLIQVDDLDLYDGPDDSDYESDDSNAENNPHFDYPDEVSEEDDELGSE 300
           + IAEDDASNPFPLIQVDDLDLYDGPDDSDYESDDSNAENNPHFDYPDE SEE+ EL SE
Sbjct: 241 VPIAEDDASNPFPLIQVDDLDLYDGPDDSDYESDDSNAENNPHFDYPDEASEEE-ELESE 300

Query: 301 SSNGESKDNDDDSEKKQSSEANDSEEDDLSVDRAQLYEDEIYGDFDDDDANSFDCDSNGG 360
           S+N ES+++D+D   KQSSEAN  EEDDLS +  QL EDEIYGDFD+DD +SFD DSNGG
Sbjct: 301 STNEESEEDDED---KQSSEANGLEEDDLSENGVQLDEDEIYGDFDEDDGDSFDYDSNGG 360

Query: 361 HDEGEDSRWSYR 372
            +EGED RWSYR
Sbjct: 361 QEEGEDWRWSYR 368

[Close]

BLAST of MC03g0028 vs. ExPASy TrEMBL
Match: A0A6J1K8G0 (RNA-directed DNA methylation 4 OS=Cucurbita maxima OX=3661 GN=LOC111493190 PE=3 SV=1)

HSP 1 Score: 562 bits (1448), Expect = 2.74e-199
Identity = 304/372 (81.72%), Postives = 336/372 (90.32%), Query Frame = 0


Query: 1   MATIGESSSSVPKLADGKPVLVRVKRKASQSRLDALWLEINERPLKRPLLDFENLSISET 60
           MA+IGESSSS PK AD KPVLVRVKRKASQSRLDALWLEINERPLKRPLLDF+NLSISET
Sbjct: 1   MASIGESSSSAPKPADEKPVLVRVKRKASQSRLDALWLEINERPLKRPLLDFDNLSISET 60

Query: 61  FQNEELKTKKIFVQHVETLKSSEATVDIVQSFVAPDAAGTVENSVKNEERRRNFKREIPR 120
           F+  ELKTKKIFVQHVETL+SSEA+VDI+QSFVAPDAAG +EN++KNEERRRNFKRE PR
Sbjct: 61  FKKAELKTKKIFVQHVETLRSSEASVDIIQSFVAPDAAGNIENNLKNEERRRNFKRESPR 120

Query: 121 QDQLLSKARQEQEVLAKNARFEQIWRSRKGVKDAKDDQLHDMYHIYDIVRLDTNKISSEV 180
           Q+QLL K RQEQEVL K ARFEQIWRSRKG+KDAKDDQLHD YHIYDIVRLDTN+ SS  
Sbjct: 121 QEQLLVKTRQEQEVLTKKARFEQIWRSRKGIKDAKDDQLHDAYHIYDIVRLDTNETSSGA 180

Query: 181 PEQEYMSLEDQSMLSSYLPLLREFIPSAAAEIESDIHANMMKQDVPVDDYVYDYYTVKSD 240
           P+ + M+LEDQSMLSSYLPLLREFIPSAA EIESDI+AN MKQD+PVDDYVYDYYTVK++
Sbjct: 181 PKADCMTLEDQSMLSSYLPLLREFIPSAAEEIESDINANTMKQDLPVDDYVYDYYTVKNN 240

Query: 241 MEIAEDDASNPFPLIQVDDLDLYDGPDDSDYESDDSNAENNPHFDYPDEVSEEDDELGSE 300
           ++IAEDDASNPFPLIQVDDLDLYDGPDDSDYESDDSNAENNPHFDYPDE SEE+ EL SE
Sbjct: 241 VQIAEDDASNPFPLIQVDDLDLYDGPDDSDYESDDSNAENNPHFDYPDEASEEE-ELESE 300

Query: 301 SSNGESKDNDDDSEKKQSSEANDSEEDDLSVDRAQLYEDEIYGDFDDDDANSFDCDSNGG 360
           S+N ES+++D+D   KQSSEAN  EEDDLS +  QL EDEIYGDFD+ D +SFD DSNGG
Sbjct: 301 STNEESEEDDED---KQSSEANGLEEDDLSENGVQLDEDEIYGDFDEGDGDSFDYDSNGG 360

Query: 361 HDEGEDSRWSYR 372
           H+EGE+ RWSYR
Sbjct: 361 HEEGEECRWSYR 368

[Close]

BLAST of MC03g0028 vs. ExPASy TrEMBL
Match: A0A5D3BU38 (RNA-directed DNA methylation 4-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1161G00230 PE=4 SV=1)

HSP 1 Score: 548 bits (1412), Expect = 6.23e-193
Identity = 306/374 (81.82%), Postives = 334/374 (89.30%), Query Frame = 0


Query: 1   MATIGESSSSVPKLADGKPVLVRVKRKASQSRLDALWLEINERPLKRPLLDFENLSISET 60
           MA IGESSSSVPKL D KPVLVRVKRKASQSRLDALWLEINERPLKRP LDFENLSISET
Sbjct: 60  MALIGESSSSVPKLVDEKPVLVRVKRKASQSRLDALWLEINERPLKRPFLDFENLSISET 119

Query: 61  FQNEELKTKKIFVQHVETLKSSEATVDIVQSFVAPDAAGTVENSVKNEERRRNFKREIPR 120
              EELKTKKIFVQHVETL+SSEAT DIVQSFVAPDAA T+EN++KNEERRRNFKREI R
Sbjct: 120 LYKEELKTKKIFVQHVETLRSSEATADIVQSFVAPDAASTIENNLKNEERRRNFKREISR 179

Query: 121 QDQLLSKARQEQEVLAKNARFEQIWRSRKGVKDAKDDQLHDMYHIYDIVRLDTNKISSEV 180
           QDQLL KARQEQE++AKNARFEQIWRSRKGVKD KDDQLHD+YHIYDIVRLDTN+ISSE 
Sbjct: 180 QDQLLVKARQEQELVAKNARFEQIWRSRKGVKDEKDDQLHDVYHIYDIVRLDTNEISSEA 239

Query: 181 PEQEYMSLEDQSMLSSYLPLLREFIPSAAAEIESDIHANMMKQDVPVDDYVYDYYTVKSD 240
           P QE MSLEDQSMLSSYLPLLREFIPSAAAEIESDI A+MMKQ++PVDDYVYDYYTVK+D
Sbjct: 240 PRQEQMSLEDQSMLSSYLPLLREFIPSAAAEIESDIDASMMKQNLPVDDYVYDYYTVKTD 299

Query: 241 MEIAEDDASNPFPLIQVDDLDLYDGPDDSDYESDDSNAENNPHFDYPDEVSEEDDELGSE 300
           +EI EDDAS+PFPLIQVDDLD +DGP DSDYE+DDSNAENNP FDYPDE     +EL S+
Sbjct: 300 VEIPEDDASHPFPLIQVDDLD-HDGPSDSDYETDDSNAENNPCFDYPDE-----EELESK 359

Query: 301 SSNGESKDNDDDSEKKQSSEANDSEEDDLSVD-RAQLYEDEIYGDFDDDD-ANSFDCDSN 360
           SSN E +D+DD   +KQSSE+ND EED+LS + + +LYEDEIY DFD+DD A+SFD DSN
Sbjct: 360 SSNDELEDSDD---EKQSSESNDVEEDELSEEEKVELYEDEIYDDFDEDDGADSFDYDSN 419

Query: 361 GGHDEGEDSRWSYR 372
           GG DEGED RWSYR
Sbjct: 420 GGRDEGEDWRWSYR 424

[Close]

BLAST of MC03g0028 vs. ExPASy TrEMBL
Match: A0A1S4DWJ0 (RNA-directed DNA methylation 4-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103489641 PE=4 SV=1)

HSP 1 Score: 545 bits (1405), Expect = 8.61e-193
Identity = 304/374 (81.28%), Postives = 333/374 (89.04%), Query Frame = 0


Query: 1   MATIGESSSSVPKLADGKPVLVRVKRKASQSRLDALWLEINERPLKRPLLDFENLSISET 60
           MA IGESSSSVPKL D KPVLVRVKRKASQSRLDALWLEINERPLKRP LDFENLSISET
Sbjct: 1   MALIGESSSSVPKLVDEKPVLVRVKRKASQSRLDALWLEINERPLKRPFLDFENLSISET 60

Query: 61  FQNEELKTKKIFVQHVETLKSSEATVDIVQSFVAPDAAGTVENSVKNEERRRNFKREIPR 120
              EELKTKKI VQHVETL+SSEAT DIVQSFVAPDAA T+EN++KNEERRRNFKREI R
Sbjct: 61  LYKEELKTKKILVQHVETLRSSEATADIVQSFVAPDAASTIENNLKNEERRRNFKREISR 120

Query: 121 QDQLLSKARQEQEVLAKNARFEQIWRSRKGVKDAKDDQLHDMYHIYDIVRLDTNKISSEV 180
           QDQLL KARQEQE++AKNARFEQIWRSRKGVKD KDDQLHD+YHIYDIVRLDTN+ISSE 
Sbjct: 121 QDQLLVKARQEQELVAKNARFEQIWRSRKGVKDEKDDQLHDVYHIYDIVRLDTNEISSEA 180

Query: 181 PEQEYMSLEDQSMLSSYLPLLREFIPSAAAEIESDIHANMMKQDVPVDDYVYDYYTVKSD 240
           P QE MSLEDQSMLSSYLPLLREFIPSAAAEIESDI  +MMKQ++PVDDYVYDYYTVK+D
Sbjct: 181 PRQEQMSLEDQSMLSSYLPLLREFIPSAAAEIESDIDPSMMKQNLPVDDYVYDYYTVKTD 240

Query: 241 MEIAEDDASNPFPLIQVDDLDLYDGPDDSDYESDDSNAENNPHFDYPDEVSEEDDELGSE 300
           +EI EDDAS+PFPLIQVDDLD +DGP DSDYE+DDSNAENNP FDYPDE     +EL S+
Sbjct: 241 VEIPEDDASHPFPLIQVDDLD-HDGPSDSDYETDDSNAENNPCFDYPDE-----EELESK 300

Query: 301 SSNGESKDNDDDSEKKQSSEANDSEEDDLSVD-RAQLYEDEIYGDFDDDD-ANSFDCDSN 360
           SSN E +D+DD   +KQSSE+ND EED+LS + + +LYEDEIY DFD+DD A+SFD DSN
Sbjct: 301 SSNDELEDSDD---EKQSSESNDVEEDELSEEEKVELYEDEIYDDFDEDDGADSFDYDSN 360

Query: 361 GGHDEGEDSRWSYR 372
           GGHD+GED RWSYR
Sbjct: 361 GGHDKGEDWRWSYR 365

[Close]

BLAST of MC03g0028 vs. TAIR 10
Match: AT2G30280.1 (RNA-directed DNA methylation 4 )

HSP 1 Score: 250.4 bits (638), Expect = 2.3e-66
Identity = 162/361 (44.88%), Postives = 231/361 (63.99%), Query Frame = 0


Query: 5   GESSSSVPKLADGKPVLVRVKRKASQSRLDALWLEINERPLKRPLLDFENLSISETFQN- 64
           G   SS     + KPV+VRVKRK  QS LDA WLEINERPLKRP LDF  LSIS++ +  
Sbjct: 3   GVGESSTQNEVEEKPVIVRVKRKVGQSLLDAFWLEINERPLKRPTLDFSKLSISDSGERG 62

Query: 65  ----EELKTKKIFVQHVETLKSSEATVDIVQSFVAPDAAGTVENSVKNEERRRNFKREIP 124
               E++K KK+ V+H+ET+  SE T DI+ SF   D      +  K EER+  FK++  
Sbjct: 63  PSVAEDVKPKKVLVRHLETVTDSETTADIIHSFFESDHNEKSCSKGKFEERKIAFKKD-N 122

Query: 125 RQDQLLSKARQEQEVLAKNARFEQIWRSRKGVKDAKDDQLHDMYHIYDIVRLDTNKISSE 184
           R++Q L+K+ Q+Q++ ++NARFEQIWRSRKG K+     +H+  H +D++R+DT +    
Sbjct: 123 RKEQRLTKSVQKQQIASENARFEQIWRSRKGNKEG----IHEKCHFFDVIRVDTEERRDN 182

Query: 185 VPEQEYMSLEDQSMLSSYLPLLREFIPSAAAEIESDIHANMMKQDVPVDDYVYDYYTVKS 244
              QE+ SLEDQ ML+S+LPLLRE IP+AA EIE+DI      Q    ++YVYDYY V  
Sbjct: 183 A--QEFTSLEDQKMLASFLPLLRECIPTAAEEIEADI------QSSHTEEYVYDYYAVNE 242

Query: 245 DMEIAEDDASNPFPLIQVDDLDLY-DGPDDSDYESDDSNAENNPHFDYPDEVSEEDDELG 304
           +M+I+ED + N FPL+ V+D + + DG D+SDY+S+DSNAE++P  DYP+E  EE++E  
Sbjct: 243 EMDISEDSSKNQFPLVIVEDEEEFCDGSDESDYDSEDSNAEDHPKTDYPEEEEEEEEE-- 302

Query: 305 SESSNGESKDNDDDSEKKQSSEANDSEEDD----------LSVDRAQLYEDEIYGDFDDD 350
                 +  D+DDD  +++ SEA+D  +D+          L  D    Y +++YG  + D
Sbjct: 303 ------DDDDDDDDESEEEKSEASDESDDEETSKRHVRSVLGDDEFDDYAEDVYGYSESD 342

The following BLAST results are available for this feature:

BLAST of MC03g0028 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8GYP3	3.3e-65	44.88	RNA-directed DNA methylation 4 OS=Arabidopsis thaliana OX=3702 GN=RDM4 PE=1 SV=1	[more]

BLAST of MC03g0028 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022154907.1	1.12e-252	100.00	RNA-directed DNA methylation 4 [Momordica charantia]	[more]
XP_023522069.1	8.87e-203	82.80	RNA-directed DNA methylation 4 [Cucurbita pepo subsp. pepo]	[more]
KAG6607356.1	8.87e-203	83.06	RNA-directed DNA methylation 4, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022949593.1	1.46e-201	82.80	RNA-directed DNA methylation 4 [Cucurbita moschata]	[more]
XP_038885561.1	3.56e-200	83.38	RNA-directed DNA methylation 4 [Benincasa hispida] >XP_038885562.1 RNA-directed ...	[more]

BLAST of MC03g0028 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DMY7	5.44e-253	100.00	RNA-directed DNA methylation 4 OS=Momordica charantia OX=3673 GN=LOC111022052 PE...	[more]
A0A6J1GCH8	7.09e-202	82.80	RNA-directed DNA methylation 4 OS=Cucurbita moschata OX=3662 GN=LOC111452902 PE=...	[more]
A0A6J1K8G0	2.74e-199	81.72	RNA-directed DNA methylation 4 OS=Cucurbita maxima OX=3661 GN=LOC111493190 PE=3 ...	[more]
A0A5D3BU38	6.23e-193	81.82	RNA-directed DNA methylation 4-like isoform X1 OS=Cucumis melo var. makuwa OX=11...	[more]
A0A1S4DWJ0	8.61e-193	81.28	RNA-directed DNA methylation 4-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103...	[more]

BLAST of MC03g0028 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G30280.1	2.3e-66	44.88	RNA-directed DNA methylation 4	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013883	Transcription factor Iwr1 domain	PFAM	PF08574	Iwr1	coord: 228..289 e-value: 1.1E-4 score: 23.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 261..275
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 258..372
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 354..372
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 300..340
None	No IPR available	PANTHER	PTHR31934	ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN	coord: 1..372
None	No IPR available	PANTHER	PTHR31934:SF2	RNA-DIRECTED DNA METHYLATION 4	coord: 1..372

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC03g0028.1	MC03g0028.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006306	DNA methylation
biological_process	GO:0009791	post-embryonic development
biological_process	GO:0006355	regulation of transcription, DNA-templated
molecular_function	GO:0003700	DNA-binding transcription factor activity