MC02g_new0218 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC02g_new0218
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	mitogen-activated protein kinase kinase kinase 17-like
Location	MC02: 9196820 .. 9198290 (-)
RNA-Seq Expression	MC02g_new0218
Synteny	MC02g_new0218

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CAAATTTTAGAACTCGGGAATCAATTCAACTTCGAACTCTGTTTCTTTTCTTCTTCCCAGTTACCAAATTAAACAGAGAAATCGAATTCGCCGGAGGAATTTCAGACTTGGGGATTGTGAGATTGGAGCTTCGAATTTCCATCAATGGAGTGGATCAGAGGAGATCAATTGGGCTCCGGCAACTTCGCAACAATCAATTTAGCGATTCTCACAAAGGAGTTCGATCAATTTCCGCCATTAATGGCGGTGAAGACCTCGCCGGCCAGCTCCTCTGTTTCGCTGAAGAACGAGAAGCAAATTTTTGATCAAATTGGAAGTTGCCCACAAATTCTTACCTGTTTTGGCGATGGCTACACCATTGAAAGGGACGGGCAGAAGCATTACAATCTGTTCTTGGAGTACGCCAATGGCGGAAGCCTTGCAGATAAAGTCAAAGCCCACGGCGGCGGGTTGCCGGAATTCGATGTTCAGAGCTACATGGCGGCGGTTCTTAATGGCCTGAGATTTATCCACGAAAATGGGTTTGTTCACTGCGATATAAAGCTTGCGAATATATTGACGTTTAGCAATGGCGGCGCGAAGATCGCTGATTTCGGGCTGGCGAAGAAGGCGGCGGCGGAAACAGAGCAGAAATTTGAGTGGAGAGGGACTCCTCTGTATATGTCGCCGGAATCTGTGAACAGCGGCGTGTATGAGCCGCCGTGTGATATCTGGGCGCTGGGTTGCGCCGTCGTGGAGATGGTGGCCGGAAAGCCGGCGTGGAATTGTAAGCCGGAAACGGACATCTTCGGGCTAATGATGCGAATCGGAGCCGGAGACGAAGTGCCCGACCCGCCGGAGAATTTGTCGGACGACGGGAAAGATTTTCTCCGAAAGTGTTTCATCAAGGATCCGACGAAGAGATGGACGGCGGAGATGCTTTTGAATCACCCGTTCGTCGTCGGAGGCGGCGATACTGTTACATTGAAGGAGACAGAACCGCCGGCGGCGTCCCCTCGTGGTCCGTTCGATTTCCCGGAATTTGGTTCGCTGCCGTTAAGCTCCGACGAGCGGTGTTTCACGCCGGCGATTGATTCGGGGTTGGATTTTGCACCGGCGATGAGTACGATCCGGCGGCTGGTGACGGAGAAGCCACTGGATTGGTCGGTTTCGGATAGTTGGGTGACAGTGAGGTGAGGTGATCAAATTTTGTGGCAGAGCAGAGGGAGCAGTCAGAAGGTGAAAGTTGAAGAAGTTTTTGGAAGATCTTCAACGCATCGGACGGTCCTGATTCAATTGATAATTTCTTTTTTTTAATTCACAATTGGGCTCTGTTTTATTTTGTTTTAATTTCTGTAAATGAGTAGTCAGCTGATTCGACGGTGCTGATTTAAAGCATCAGAGTCAGATATTACTTAGTGGTGTAGGAATTTGTACAATATAGTTTATAATTTATAAATATATATATACACACACTTTTGTTACAATAAGG

mRNA sequence

ATGGAGTGGATCAGAGGAGATCAATTGGGCTCCGGCAACTTCGCAACAATCAATTTAGCGATTCTCACAAAGGAGTTCGATCAATTTCCGCCATTAATGGCGGTGAAGACCTCGCCGGCCAGCTCCTCTGTTTCGCTGAAGAACGAGAAGCAAATTTTTGATCAAATTGGAAGTTGCCCACAAATTCTTACCTGTTTTGGCGATGGCTACACCATTGAAAGGGACGGGCAGAAGCATTACAATCTGTTCTTGGAGTACGCCAATGGCGGAAGCCTTGCAGATAAAGTCAAAGCCCACGGCGGCGGGTTGCCGGAATTCGATGTTCAGAGCTACATGGCGGCGGTTCTTAATGGCCTGAGATTTATCCACGAAAATGGGTTTGTTCACTGCGATATAAAGCTTGCGAATATATTGACGTTTAGCAATGGCGGCGCGAAGATCGCTGATTTCGGGCTGGCGAAGAAGGCGGCGGCGGAAACAGAGCAGAAATTTGAGTGGAGAGGGACTCCTCTGTATATGTCGCCGGAATCTGTGAACAGCGGCGTGTATGAGCCGCCGTGTGATATCTGGGCGCTGGGTTGCGCCGTCGTGGAGATGGTGGCCGGAAAGCCGGCGTGGAATTGTAAGCCGGAAACGGACATCTTCGGGCTAATGATGCGAATCGGAGCCGGAGACGAAGTGCCCGACCCGCCGGAGAATTTGTCGGACGACGGGAAAGATTTTCTCCGAAAGTGTTTCATCAAGGATCCGACGAAGAGATGGACGGCGGAGATGCTTTTGAATCACCCGTTCGTCGTCGGAGGCGGCGATACTGTTACATTGAAGGAGACAGAACCGCCGGCGGCGTCCCCTCGTGGTCCGTTCGATTTCCCGGAATTTGGTTCGCTGCCGTTAAGCTCCGACGAGCGGTGTTTCACGCCGGCGATTGATTCGGGGTTGGATTTTGCACCGGCGATGAGTACGATCCGGCGGCTGGTGACGGAGAAGCCACTGGATTGGTCGGTTTCGGATAGTTGGGTGACAGTGAGGTGA

Coding sequence (CDS)

ATGGAGTGGATCAGAGGAGATCAATTGGGCTCCGGCAACTTCGCAACAATCAATTTAGCGATTCTCACAAAGGAGTTCGATCAATTTCCGCCATTAATGGCGGTGAAGACCTCGCCGGCCAGCTCCTCTGTTTCGCTGAAGAACGAGAAGCAAATTTTTGATCAAATTGGAAGTTGCCCACAAATTCTTACCTGTTTTGGCGATGGCTACACCATTGAAAGGGACGGGCAGAAGCATTACAATCTGTTCTTGGAGTACGCCAATGGCGGAAGCCTTGCAGATAAAGTCAAAGCCCACGGCGGCGGGTTGCCGGAATTCGATGTTCAGAGCTACATGGCGGCGGTTCTTAATGGCCTGAGATTTATCCACGAAAATGGGTTTGTTCACTGCGATATAAAGCTTGCGAATATATTGACGTTTAGCAATGGCGGCGCGAAGATCGCTGATTTCGGGCTGGCGAAGAAGGCGGCGGCGGAAACAGAGCAGAAATTTGAGTGGAGAGGGACTCCTCTGTATATGTCGCCGGAATCTGTGAACAGCGGCGTGTATGAGCCGCCGTGTGATATCTGGGCGCTGGGTTGCGCCGTCGTGGAGATGGTGGCCGGAAAGCCGGCGTGGAATTGTAAGCCGGAAACGGACATCTTCGGGCTAATGATGCGAATCGGAGCCGGAGACGAAGTGCCCGACCCGCCGGAGAATTTGTCGGACGACGGGAAAGATTTTCTCCGAAAGTGTTTCATCAAGGATCCGACGAAGAGATGGACGGCGGAGATGCTTTTGAATCACCCGTTCGTCGTCGGAGGCGGCGATACTGTTACATTGAAGGAGACAGAACCGCCGGCGGCGTCCCCTCGTGGTCCGTTCGATTTCCCGGAATTTGGTTCGCTGCCGTTAAGCTCCGACGAGCGGTGTTTCACGCCGGCGATTGATTCGGGGTTGGATTTTGCACCGGCGATGAGTACGATCCGGCGGCTGGTGACGGAGAAGCCACTGGATTGGTCGGTTTCGGATAGTTGGGTGACAGTGAGGTGA

Protein sequence

MEWIRGDQLGSGNFATINLAILTKEFDQFPPLMAVKTSPASSSVSLKNEKQIFDQIGSCPQILTCFGDGYTIERDGQKHYNLFLEYANGGSLADKVKAHGGGLPEFDVQSYMAAVLNGLRFIHENGFVHCDIKLANILTFSNGGAKIADFGLAKKAAAETEQKFEWRGTPLYMSPESVNSGVYEPPCDIWALGCAVVEMVAGKPAWNCKPETDIFGLMMRIGAGDEVPDPPENLSDDGKDFLRKCFIKDPTKRWTAEMLLNHPFVVGGGDTVTLKETEPPAASPRGPFDFPEFGSLPLSSDERCFTPAIDSGLDFAPAMSTIRRLVTEKPLDWSVSDSWVTVR

Homology

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BLAST of MC02g_new0218 vs. ExPASy Swiss-Prot
Match: Q9SND6 (Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK20 PE=1 SV=1)

HSP 1 Score: 331.6 bits (849), Expect = 1.0e-89
Identity = 168/354 (47.46%), Postives = 226/354 (63.84%), Query Frame = 0


Query: 1   MEWIRGDQLGSGNFATINLAILTKEFDQFPPLMAVKTSPASSSVSLKNEKQIFDQIGSCP 60
           MEW+RG+ +G G F+T++ A  ++    FP L+AVK++ A  + SL NEK + D +G CP
Sbjct: 1   MEWVRGETIGFGTFSTVSTATKSRNSGDFPALIAVKSTDAYGAASLSNEKSVLDSLGDCP 60

Query: 61  QILTCFGDGYTIERDGQKHYNLFLEYANGGSLADKVKAHGG-GLPEFDVQSYMAAVLNGL 120
           +I+ C+G+  T+E +G++ +NL LEYA+ GSLA  +K  GG GLPE  V+ +  +VL GL
Sbjct: 61  EIIRCYGEDSTVE-NGEEMHNLLLEYASRGSLASYMKKLGGEGLPESTVRRHTGSVLRGL 120

Query: 121 RFIHENGFVHCDIKLANILTFSNGGAKIADFGLAKKAAAE---TEQKFEWRGTPLYMSPE 180
           R IH  GF HCDIKLANIL F++G  KIADFGLA +   +     +  E RGTPLYM+PE
Sbjct: 121 RHIHAKGFAHCDIKLANILLFNDGSVKIADFGLAMRVDGDLTALRKSVEIRGTPLYMAPE 180

Query: 181 SVNSGVYEPPCDIWALGCAVVEMVAGKPAWNCKPETDIFGLMMRIGAGDEVPDPPENLSD 240
            VN   Y    D+WALGCAVVEM +GK AW+ K  +    L++RIG GDE+P  PE LS+
Sbjct: 181 CVNDNEYGSAADVWALGCAVVEMFSGKTAWSVKEGSHFMSLLIRIGVGDELPKIPEMLSE 240

Query: 241 DGKDFLRKCFIKDPTKRWTAEMLLNHPFVV-------GGGDTVTLKETEPPAASPRGPFD 300
           +GKDFL KCF+KDP KRWTAEMLLNH FV             V +K+ +    SP+ PF+
Sbjct: 241 EGKDFLSKCFVKDPAKRWTAEMLLNHSFVTIDLEDDHRENFVVKVKDEDKVLMSPKCPFE 300

Query: 301 FPEFGSLPLSSDERCFTPAIDSGLDFAPAMSTIRRLVTEKPLDWSVSDSWVTVR 344
           F ++ S  L S+     P+ DS ++       +  LV+    DWSV  SW+TVR
Sbjct: 301 FDDWDSFTLDSN-----PSFDSPVE------RLGSLVSGSIPDWSVGGSWLTVR 342

[Close]

BLAST of MC02g_new0218 vs. ExPASy Swiss-Prot
Match: O80888 (Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK17 PE=1 SV=1)

HSP 1 Score: 189.9 bits (481), Expect = 4.8e-47
Identity = 103/266 (38.72%), Postives = 141/266 (53.01%), Query Frame = 0


Query: 1   MEWIRGDQLGSGNFATINLAILTKEFDQFPPLMAVKTSPASSSVSLKNEKQIFDQIGSCP 60
           MEW RG  LG G+ AT+  A      D+   ++AVK+S    S  L+ E +I   + S P
Sbjct: 1   MEWTRGRILGRGSTATV-YAAAGHNSDE---ILAVKSSEVHRSEFLQREAKILSSLSS-P 60

Query: 61  QILTCFGDGYTIERDGQKHYNLFLEYANGGSLADKVKAHGGGLPEFDVQSYMAAVLNGLR 120
            ++   G     E +G   YNL +EYA  G+L D     GG + E  V  Y   +L GL 
Sbjct: 61  YVIGYRGSETKRESNGVVMYNLLMEYAPYGTLTDAAAKDGGRVDETRVVKYTRDILKGLE 120

Query: 121 FIHENGFVHCDIKLANILTFSNGGAKIADFGLAKKAAAETEQKFEWRGTPLYMSPESVNS 180
           +IH  G VHCD+K +N++    G AKIADFG AK+     E      GTP +M+PE    
Sbjct: 121 YIHSKGIVHCDVKGSNVVISEKGEAKIADFGCAKRVDPVFESPV--MGTPAFMAPEVARG 180

Query: 181 GVYEPPCDIWALGCAVVEMVAGKPAW-NCKPETDIFGLMMRIGAGDEVPDPPENLSDDGK 240
                  DIWA+GC ++EMV G P W       D   ++ R+G   E P+ P  L+++ K
Sbjct: 181 EKQGKESDIWAVGCTMIEMVTGSPPWTKADSREDPVSVLYRVGYSSETPELPCLLAEEAK 240

Query: 241 DFLRKCFIKDPTKRWTAEMLLNHPFV 266
           DFL KC  ++  +RWTA  LLNHPF+
Sbjct: 241 DFLEKCLKREANERWTATQLLNHPFL 259

[Close]

BLAST of MC02g_new0218 vs. ExPASy Swiss-Prot
Match: Q9ZVP5 (Mitogen-activated protein kinase kinase kinase 18 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK18 PE=1 SV=1)

HSP 1 Score: 186.8 bits (473), Expect = 4.1e-46
Identity = 107/271 (39.48%), Postives = 149/271 (54.98%), Query Frame = 0


Query: 1   MEWIRGDQLGSGNFATINLAILTKEFDQFPPLMAVKTSPASSSVSLKNEKQIFDQIGSCP 60
           M W RG  LG G+ AT++ A   +  +     +AVK++    S  L+ E +I   + S P
Sbjct: 1   MNWTRGKTLGRGSTATVSAATCHESGE----TLAVKSAEFHRSEFLQREAKILSSLNS-P 60

Query: 61  QILTCFGDGYTIE---RDGQ-KHYNLFLEYANGGSLADKVKAHGGGLPEFDVQSYMAAVL 120
            ++   G   T E    +G+   Y+L +EYA  G+L D    +GG + E  V  Y   +L
Sbjct: 61  YVIGYRGCEITREPFHNNGEATTYSLLMEYAPYGTLTDVATKNGGFIDEARVVKYTRQIL 120

Query: 121 NGLRFIHEN-GFVHCDIKLANILTFSNGGAKIADFGLAKKAAAETEQKFEWRGTPLYMSP 180
            GL +IH + G  HCDIK +N+L   NG AKIADFG AK    E  +    RGTP +M+P
Sbjct: 121 LGLEYIHNSKGIAHCDIKGSNVLVGENGEAKIADFGCAKWVEPEITEPV--RGTPAFMAP 180

Query: 181 ESVNSGVYEPPCDIWALGCAVVEMVAGKPAWNCKPETDIFGLMMRIGAGDEVPDPPENLS 240
           E+          DIWA+GC V+EMV G   W     TD   ++ R+G   E+P+ P +L+
Sbjct: 181 EAARGERQGKESDIWAVGCTVIEMVTGSQPWIGADFTDPVSVLYRVGYLGELPELPCSLT 240

Query: 241 DDGKDFLRKCFIKDPTKRWTAEMLLNHPFVV 267
           +  KDFL KC  K+ T+RWTA  LLNHPF+V
Sbjct: 241 EQAKDFLGKCLKKEATERWTASQLLNHPFLV 264

[Close]

BLAST of MC02g_new0218 vs. ExPASy Swiss-Prot
Match: F4HRJ4 (Mitogen-activated protein kinase kinase kinase 3 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK3 PE=1 SV=1)

HSP 1 Score: 156.0 bits (393), Expect = 7.7e-37
Identity = 97/292 (33.22%), Postives = 151/292 (51.71%), Query Frame = 0


Query: 3   WIRGDQLGSGNFATINLAILTKEFDQFPPLMAVK--TSPASSSVSLKNEKQIFDQIGSC- 62
           W +G  LGSG F  + L     E  +   +  VK  +   +S   LK   Q  + +    
Sbjct: 214 WKKGKFLGSGTFGQVYLG-FNSEKGKMCAIKEVKVISDDQTSKECLKQLNQEINLLNQLC 273

Query: 63  -PQILTCFGDGYTIERDGQKHYNLFLEYANGGSLADKVKAHGGGLPEFDVQSYMAAVLNG 122
            P I+  +G   +     ++  +++LEY +GGS+   +K + G   E  +Q+Y   +L G
Sbjct: 274 HPNIVQYYGSELS-----EETLSVYLEYVSGGSIHKLLKDY-GSFTEPVIQNYTRQILAG 333

Query: 123 LRFIHENGFVHCDIKLANILTFSNGGAKIADFGLAKKAAAETEQKFEWRGTPLYMSPESV 182
           L ++H    VH DIK ANIL   NG  K+ADFG+AK   A +     ++G+P +M+PE V
Sbjct: 334 LAYLHGRNTVHRDIKGANILVDPNGEIKLADFGMAKHVTAFSTM-LSFKGSPYWMAPEVV 393

Query: 183 NS-GVYEPPCDIWALGCAVVEMVAGKPAWNCKPETDIFGLMMRIGAGDEVPDPPENLSDD 242
            S   Y    DIW+LGC ++EM   KP W+   + +    + +IG   + P+ P++LS+D
Sbjct: 394 MSQNGYTHAVDIWSLGCTILEMATSKPPWS---QFEGVAAIFKIGNSKDTPEIPDHLSND 453

Query: 243 GKDFLRKCFIKDPTKRWTAEMLLNHPFVVGGGDTVTLKETEPPAASPRGPFD 290
            K+F+R C  ++PT R TA  LL HPF+    +T  +  T  P   P   +D
Sbjct: 454 AKNFIRLCLQRNPTVRPTASQLLEHPFL---RNTTRVASTSLPKDFPPRSYD 491

[Close]

BLAST of MC02g_new0218 vs. ExPASy Swiss-Prot
Match: O22042 (Mitogen-activated protein kinase kinase kinase 3 OS=Arabidopsis thaliana OX=3702 GN=ANP3 PE=1 SV=1)

HSP 1 Score: 154.5 bits (389), Expect = 2.2e-36
Identity = 91/277 (32.85%), Postives = 142/277 (51.26%), Query Frame = 0


Query: 1   MEWIRGDQLGSGNFATINLAILTKEFDQFPPLMAVKTSPASSSV--------SLKNEKQI 60
           + W +G+ +G G F  + +  +  +  +   +  V  +P+S+S          L+ E Q+
Sbjct: 66  IRWRKGELIGCGAFGRVYMG-MNLDSGELLAIKQVLIAPSSASKEKTQGHIRELEEEVQL 125

Query: 61  FDQIGSCPQILTCFGDGYTIERDGQKHYNLFLEYANGGSLADKVKAHGGGLPEFDVQSYM 120
              + S P I+   G   T+        N+ +E+  GGS++  ++   G  PE  +  Y 
Sbjct: 126 LKNL-SHPNIVRYLG---TVRESDS--LNILMEFVPGGSISSLLEKF-GSFPEPVIIMYT 185

Query: 121 AAVLNGLRFIHENGFVHCDIKLANILTFSNGGAKIADFGLAKKAA--AETEQKFEWRGTP 180
             +L GL ++H NG +H DIK ANIL  + G  ++ADFG +KK    A        +GTP
Sbjct: 186 KQLLLGLEYLHNNGIMHRDIKGANILVDNKGCIRLADFGASKKVVELATVNGAKSMKGTP 245

Query: 181 LYMSPESVNSGVYEPPCDIWALGCAVVEMVAGKPAWNCKPETDIFGLMMRIGAGDEVPDP 240
            +M+PE +    +    DIW++GC V+EM  GKP W+   +   F  ++ IG     P  
Sbjct: 246 YWMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWS--EQYQQFAAVLHIGRTKAHPPI 305

Query: 241 PENLSDDGKDFLRKCFIKDPTKRWTAEMLLNHPFVVG 268
           PE+LS + KDFL KC  K+P+ R +A  LL HPFV G
Sbjct: 306 PEDLSPEAKDFLMKCLHKEPSLRLSATELLQHPFVTG 332

[Close]

BLAST of MC02g_new0218 vs. NCBI nr
Match: XP_022149525.1 (mitogen-activated protein kinase kinase kinase 17-like [Momordica charantia])

HSP 1 Score: 721 bits (1860), Expect = 1.18e-262
Identity = 343/343 (100.00%), Postives = 343/343 (100.00%), Query Frame = 0


Query: 1   MEWIRGDQLGSGNFATINLAILTKEFDQFPPLMAVKTSPASSSVSLKNEKQIFDQIGSCP 60
           MEWIRGDQLGSGNFATINLAILTKEFDQFPPLMAVKTSPASSSVSLKNEKQIFDQIGSCP
Sbjct: 1   MEWIRGDQLGSGNFATINLAILTKEFDQFPPLMAVKTSPASSSVSLKNEKQIFDQIGSCP 60

Query: 61  QILTCFGDGYTIERDGQKHYNLFLEYANGGSLADKVKAHGGGLPEFDVQSYMAAVLNGLR 120
           QILTCFGDGYTIERDGQKHYNLFLEYANGGSLADKVKAHGGGLPEFDVQSYMAAVLNGLR
Sbjct: 61  QILTCFGDGYTIERDGQKHYNLFLEYANGGSLADKVKAHGGGLPEFDVQSYMAAVLNGLR 120

Query: 121 FIHENGFVHCDIKLANILTFSNGGAKIADFGLAKKAAAETEQKFEWRGTPLYMSPESVNS 180
           FIHENGFVHCDIKLANILTFSNGGAKIADFGLAKKAAAETEQKFEWRGTPLYMSPESVNS
Sbjct: 121 FIHENGFVHCDIKLANILTFSNGGAKIADFGLAKKAAAETEQKFEWRGTPLYMSPESVNS 180

Query: 181 GVYEPPCDIWALGCAVVEMVAGKPAWNCKPETDIFGLMMRIGAGDEVPDPPENLSDDGKD 240
           GVYEPPCDIWALGCAVVEMVAGKPAWNCKPETDIFGLMMRIGAGDEVPDPPENLSDDGKD
Sbjct: 181 GVYEPPCDIWALGCAVVEMVAGKPAWNCKPETDIFGLMMRIGAGDEVPDPPENLSDDGKD 240

Query: 241 FLRKCFIKDPTKRWTAEMLLNHPFVVGGGDTVTLKETEPPAASPRGPFDFPEFGSLPLSS 300
           FLRKCFIKDPTKRWTAEMLLNHPFVVGGGDTVTLKETEPPAASPRGPFDFPEFGSLPLSS
Sbjct: 241 FLRKCFIKDPTKRWTAEMLLNHPFVVGGGDTVTLKETEPPAASPRGPFDFPEFGSLPLSS 300

Query: 301 DERCFTPAIDSGLDFAPAMSTIRRLVTEKPLDWSVSDSWVTVR 343
           DERCFTPAIDSGLDFAPAMSTIRRLVTEKPLDWSVSDSWVTVR
Sbjct: 301 DERCFTPAIDSGLDFAPAMSTIRRLVTEKPLDWSVSDSWVTVR 343

[Close]

BLAST of MC02g_new0218 vs. NCBI nr
Match: XP_022149480.1 (mitogen-activated protein kinase kinase kinase 18-like [Momordica charantia])

HSP 1 Score: 536 bits (1380), Expect = 1.90e-189
Identity = 259/349 (74.21%), Postives = 289/349 (82.81%), Query Frame = 0


Query: 1   MEWIRGDQLGSGNFATINLAILTKEFDQFPPLMAVKTSPASSSVS--LKNEKQIFDQIGS 60
           +EW+RG++LG GNFATINLAI TK FDQFPPLMAVK+S A+SSVS  LKNEKQI D+IG+
Sbjct: 3   LEWVRGEELGRGNFATINLAIPTKGFDQFPPLMAVKSSVAASSVSVSLKNEKQILDRIGN 62

Query: 61  CPQILTCFGDGYTIERDGQKHYNLFLEYANGGSLADKVKAHGGGLPEFDVQSYMAAVLNG 120
           CPQI++CFGDG+TIERDG+K+YN+ LEYANGGSLAD VK HGGGLPEFDV+ Y AA+L G
Sbjct: 63  CPQIVSCFGDGFTIERDGEKYYNVLLEYANGGSLADSVKIHGGGLPEFDVRRYTAAILRG 122

Query: 121 LRFIHENGFVHCDIKLANILTFSNGGAKIADFGLAK----KAAAETEQKFEWRGTPLYMS 180
           LR +H NGFVHCD+KLAN+L F NG  KIADFGLAK    K AAETEQ+FEWRGTP+ MS
Sbjct: 123 LRHVHGNGFVHCDLKLANVLNFGNGDVKIADFGLAKVAGKKTAAETEQRFEWRGTPMSMS 182

Query: 181 PESVNSGVYEPPCDIWALGCAVVEMVAGKPAWNCKPETDIFGLMMRIGAGDEVPDPPENL 240
           PE+V  G YEPPCDIWALGCAVVEMVAGKPAWN +PETDIFGLMMRIG GDEVP+ PENL
Sbjct: 183 PETVTGGEYEPPCDIWALGCAVVEMVAGKPAWNVRPETDIFGLMMRIGVGDEVPELPENL 242

Query: 241 SDDGKDFLRKCFIKDPTKRWTAEMLLNHPFVVGGGDTVTLKETEPPAASPRGPFDFPEFG 300
           SD+G+DFLR+CF+KDP KRWTAEMLLNHPFV G   TVTLKE EPP  SP GPFDFPE  
Sbjct: 243 SDEGRDFLRRCFVKDPRKRWTAEMLLNHPFVAG--ITVTLKEAEPPMESPTGPFDFPE-- 302

Query: 301 SLPLSSDERCFTPAIDSGLDFAPAMSTIRRLVTEKPLDWSVSDSWVTVR 343
           SLP  SD+        S       MS +RRLVTEKPLDWSVSDSWVTVR
Sbjct: 303 SLPTCSDQPSDDSYFCSSTVSPETMSRLRRLVTEKPLDWSVSDSWVTVR 347

[Close]

BLAST of MC02g_new0218 vs. NCBI nr
Match: XP_022948206.1 (mitogen-activated protein kinase kinase kinase 18-like [Cucurbita moschata])

HSP 1 Score: 523 bits (1346), Expect = 2.74e-184
Identity = 246/349 (70.49%), Postives = 288/349 (82.52%), Query Frame = 0


Query: 1   MEWIRGDQLGSGNFATINLAILTKEFDQFPPLMAVKTSPASS--SVSLKNEKQIFDQIGS 60
           MEW+RGD+LG GNF TI+LAILTK FDQFPPLMAVK+S + S  SVSLKNEK++ D+IG+
Sbjct: 1   MEWVRGDELGRGNFGTISLAILTKGFDQFPPLMAVKSSVSVSVSSVSLKNEKKVLDRIGN 60

Query: 61  CPQILTCFGDGYTIERDGQKHYNLFLEYANGGSLADKVKAHGGGLPEFDVQSYMAAVLNG 120
           CPQI+TCFGDG+++ERDG+K+YNLFLEYANGGSLAD VK HGGGL EFDV+ Y  A+L G
Sbjct: 61  CPQIVTCFGDGFSVERDGEKYYNLFLEYANGGSLADTVKLHGGGLLEFDVRRYTRAILCG 120

Query: 121 LRFIHENGFVHCDIKLANILTFSNGGAKIADFGLAKKAAA----ETEQKFEWRGTPLYMS 180
           LR +H NGFVHCD+KL+N+L F NG  KIADFGLAK+AAA    ETE+KFEWRGTP+YMS
Sbjct: 121 LRHVHGNGFVHCDLKLSNVLVFGNGEVKIADFGLAKEAAAAETEETEEKFEWRGTPMYMS 180

Query: 181 PESVNSGVYEPPCDIWALGCAVVEMVAGKPAWNCKPETDIFGLMMRIGAGDEVPDPPENL 240
           PE+VN G Y PPCD+WALGCAVVEMV GKPAW  KPETDIFGLMM IG GDEVP+ PENL
Sbjct: 181 PETVNGGKYAPPCDVWALGCAVVEMVGGKPAWRVKPETDIFGLMMMIGVGDEVPELPENL 240

Query: 241 SDDGKDFLRKCFIKDPTKRWTAEMLLNHPFVVGGGDTVTLKETEPPAASPRGPFDFPEFG 300
           S++GKDFLR+CF+KDP KRWTAEMLLNHPFV G G+TVTLKE+     SP GPFDFPEF 
Sbjct: 241 SEEGKDFLRRCFVKDPRKRWTAEMLLNHPFVAGAGETVTLKESNLTTESPTGPFDFPEFI 300

Query: 301 SLPLSSDERCFTPAIDSGLDFAPAMSTIRRLVTEKPLDWSVSDSWVTVR 343
               SS++RC   ++      A  M+ +RR+VTEKPLDWS ++SWVTVR
Sbjct: 301 ---CSSNQRCEEMSLCCSSSTAEMMNRLRRVVTEKPLDWSFTESWVTVR 346

[Close]

BLAST of MC02g_new0218 vs. NCBI nr
Match: KAG6605205.1 (Mitogen-activated protein kinase kinase kinase 20, partial [Cucurbita argyrosperma subsp. sororia] >KAG7035183.1 Mitogen-activated protein kinase kinase kinase 17, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 521 bits (1343), Expect = 7.85e-184
Identity = 246/349 (70.49%), Postives = 287/349 (82.23%), Query Frame = 0


Query: 1   MEWIRGDQLGSGNFATINLAILTKEFDQFPPLMAVKTSPASS--SVSLKNEKQIFDQIGS 60
           MEW+RGD+LG GNF TI+LAILTK FDQFPPLMAVK+S + S  SVSLKNEK++ D+IG+
Sbjct: 1   MEWVRGDELGRGNFGTISLAILTKGFDQFPPLMAVKSSVSVSVSSVSLKNEKKVLDRIGN 60

Query: 61  CPQILTCFGDGYTIERDGQKHYNLFLEYANGGSLADKVKAHGGGLPEFDVQSYMAAVLNG 120
           CPQI+TC+GDG+++ERDG+K+YNLFLEYANGGSLAD VK HGGGL EFDV+ Y  A+L G
Sbjct: 61  CPQIVTCYGDGFSVERDGEKYYNLFLEYANGGSLADTVKLHGGGLLEFDVRRYTRAILCG 120

Query: 121 LRFIHENGFVHCDIKLANILTFSNGGAKIADFGLAKKAAA----ETEQKFEWRGTPLYMS 180
           LR +H NGFVHCD+KL+N+L F NG  KIADFGLAK+AA     ETE+KFEWRGTP+YMS
Sbjct: 121 LRHVHGNGFVHCDLKLSNVLVFGNGEVKIADFGLAKEAAPAETEETEEKFEWRGTPMYMS 180

Query: 181 PESVNSGVYEPPCDIWALGCAVVEMVAGKPAWNCKPETDIFGLMMRIGAGDEVPDPPENL 240
           PE+VN G Y PPCD+WALGCAVVEMV GKPAW  KPETDIFGLMM IG GDEVP+ PENL
Sbjct: 181 PETVNGGKYAPPCDVWALGCAVVEMVGGKPAWRVKPETDIFGLMMMIGVGDEVPELPENL 240

Query: 241 SDDGKDFLRKCFIKDPTKRWTAEMLLNHPFVVGGGDTVTLKETEPPAASPRGPFDFPEFG 300
           S++GKDFLR+CF+KDP KRWTAEMLLNHPFV G G+TVTLKE+     SP GPFDFPEF 
Sbjct: 241 SEEGKDFLRRCFVKDPRKRWTAEMLLNHPFVAGAGETVTLKESNLTTESPTGPFDFPEFI 300

Query: 301 SLPLSSDERCFTPAIDSGLDFAPAMSTIRRLVTEKPLDWSVSDSWVTVR 343
               SS++RC    +      A  M+ +RRLVTEKPLDWSV++SWVTVR
Sbjct: 301 ---CSSNQRCDEMNLCCSSSTAEMMNRLRRLVTEKPLDWSVTESWVTVR 346

[Close]

BLAST of MC02g_new0218 vs. NCBI nr
Match: XP_023007353.1 (mitogen-activated protein kinase kinase kinase 18-like [Cucurbita maxima])

HSP 1 Score: 515 bits (1326), Expect = 3.04e-181
Identity = 243/349 (69.63%), Postives = 285/349 (81.66%), Query Frame = 0


Query: 1   MEWIRGDQLGSGNFATINLAILTKEFDQFPPLMAVKTSPASS--SVSLKNEKQIFDQIGS 60
           MEW+RGD+LG GNF TI+LA LTK FDQFPPLMAVK+S + S  S SLKNEK++ D+IG+
Sbjct: 1   MEWVRGDELGRGNFGTISLARLTKGFDQFPPLMAVKSSVSVSVSSASLKNEKKVLDRIGN 60

Query: 61  CPQILTCFGDGYTIERDGQKHYNLFLEYANGGSLADKVKAHGGGLPEFDVQSYMAAVLNG 120
           CPQI+TC+GDG+++ERDG+K+YNLFLEYANGGSLAD VK HGGGL EFDV+ Y  A+L G
Sbjct: 61  CPQIVTCYGDGFSVERDGEKYYNLFLEYANGGSLADTVKLHGGGLLEFDVRRYTRAILCG 120

Query: 121 LRFIHENGFVHCDIKLANILTFSNGGAKIADFGLAKKAAA----ETEQKFEWRGTPLYMS 180
           LR +H NGFVHCD+KL+N+L F NG  KIADFGLAK+AAA    ETE+ FEWRGTP+YMS
Sbjct: 121 LRHVHGNGFVHCDLKLSNVLVFGNGEVKIADFGLAKEAAAAETEETEENFEWRGTPMYMS 180

Query: 181 PESVNSGVYEPPCDIWALGCAVVEMVAGKPAWNCKPETDIFGLMMRIGAGDEVPDPPENL 240
           PE+VN G Y PPCD+WALGCAVVEMV GKPAW  KPETDIFGLMM IG GDEVP+ PENL
Sbjct: 181 PETVNGGKYAPPCDVWALGCAVVEMVRGKPAWRVKPETDIFGLMMMIGIGDEVPELPENL 240

Query: 241 SDDGKDFLRKCFIKDPTKRWTAEMLLNHPFVVGGGDTVTLKETEPPAASPRGPFDFPEFG 300
           S++GK+FLR+CF+KDP KRWTAEMLLNHPFV G G+TVTLKE+     SP GPFDFPEF 
Sbjct: 241 SEEGKNFLRRCFVKDPRKRWTAEMLLNHPFVAGAGETVTLKESNLSTESPTGPFDFPEFV 300

Query: 301 SLPLSSDERCFTPAIDSGLDFAPAMSTIRRLVTEKPLDWSVSDSWVTVR 343
               SS++RC    +      A  M+ +RRLVTEKPLDWSV++SWVTVR
Sbjct: 301 ---CSSNQRCDEMNLCCSSSSAEMMNRLRRLVTEKPLDWSVTESWVTVR 346

[Close]

BLAST of MC02g_new0218 vs. ExPASy TrEMBL
Match: A0A6J1D5Z2 (mitogen-activated protein kinase kinase kinase 17-like OS=Momordica charantia OX=3673 GN=LOC111017937 PE=3 SV=1)

HSP 1 Score: 721 bits (1860), Expect = 5.73e-263
Identity = 343/343 (100.00%), Postives = 343/343 (100.00%), Query Frame = 0


Query: 1   MEWIRGDQLGSGNFATINLAILTKEFDQFPPLMAVKTSPASSSVSLKNEKQIFDQIGSCP 60
           MEWIRGDQLGSGNFATINLAILTKEFDQFPPLMAVKTSPASSSVSLKNEKQIFDQIGSCP
Sbjct: 1   MEWIRGDQLGSGNFATINLAILTKEFDQFPPLMAVKTSPASSSVSLKNEKQIFDQIGSCP 60

Query: 61  QILTCFGDGYTIERDGQKHYNLFLEYANGGSLADKVKAHGGGLPEFDVQSYMAAVLNGLR 120
           QILTCFGDGYTIERDGQKHYNLFLEYANGGSLADKVKAHGGGLPEFDVQSYMAAVLNGLR
Sbjct: 61  QILTCFGDGYTIERDGQKHYNLFLEYANGGSLADKVKAHGGGLPEFDVQSYMAAVLNGLR 120

Query: 121 FIHENGFVHCDIKLANILTFSNGGAKIADFGLAKKAAAETEQKFEWRGTPLYMSPESVNS 180
           FIHENGFVHCDIKLANILTFSNGGAKIADFGLAKKAAAETEQKFEWRGTPLYMSPESVNS
Sbjct: 121 FIHENGFVHCDIKLANILTFSNGGAKIADFGLAKKAAAETEQKFEWRGTPLYMSPESVNS 180

Query: 181 GVYEPPCDIWALGCAVVEMVAGKPAWNCKPETDIFGLMMRIGAGDEVPDPPENLSDDGKD 240
           GVYEPPCDIWALGCAVVEMVAGKPAWNCKPETDIFGLMMRIGAGDEVPDPPENLSDDGKD
Sbjct: 181 GVYEPPCDIWALGCAVVEMVAGKPAWNCKPETDIFGLMMRIGAGDEVPDPPENLSDDGKD 240

Query: 241 FLRKCFIKDPTKRWTAEMLLNHPFVVGGGDTVTLKETEPPAASPRGPFDFPEFGSLPLSS 300
           FLRKCFIKDPTKRWTAEMLLNHPFVVGGGDTVTLKETEPPAASPRGPFDFPEFGSLPLSS
Sbjct: 241 FLRKCFIKDPTKRWTAEMLLNHPFVVGGGDTVTLKETEPPAASPRGPFDFPEFGSLPLSS 300

Query: 301 DERCFTPAIDSGLDFAPAMSTIRRLVTEKPLDWSVSDSWVTVR 343
           DERCFTPAIDSGLDFAPAMSTIRRLVTEKPLDWSVSDSWVTVR
Sbjct: 301 DERCFTPAIDSGLDFAPAMSTIRRLVTEKPLDWSVSDSWVTVR 343

[Close]

BLAST of MC02g_new0218 vs. ExPASy TrEMBL
Match: A0A6J1D5U5 (mitogen-activated protein kinase kinase kinase 18-like OS=Momordica charantia OX=3673 GN=LOC111017899 PE=4 SV=1)

HSP 1 Score: 536 bits (1380), Expect = 9.19e-190
Identity = 259/349 (74.21%), Postives = 289/349 (82.81%), Query Frame = 0


Query: 1   MEWIRGDQLGSGNFATINLAILTKEFDQFPPLMAVKTSPASSSVS--LKNEKQIFDQIGS 60
           +EW+RG++LG GNFATINLAI TK FDQFPPLMAVK+S A+SSVS  LKNEKQI D+IG+
Sbjct: 3   LEWVRGEELGRGNFATINLAIPTKGFDQFPPLMAVKSSVAASSVSVSLKNEKQILDRIGN 62

Query: 61  CPQILTCFGDGYTIERDGQKHYNLFLEYANGGSLADKVKAHGGGLPEFDVQSYMAAVLNG 120
           CPQI++CFGDG+TIERDG+K+YN+ LEYANGGSLAD VK HGGGLPEFDV+ Y AA+L G
Sbjct: 63  CPQIVSCFGDGFTIERDGEKYYNVLLEYANGGSLADSVKIHGGGLPEFDVRRYTAAILRG 122

Query: 121 LRFIHENGFVHCDIKLANILTFSNGGAKIADFGLAK----KAAAETEQKFEWRGTPLYMS 180
           LR +H NGFVHCD+KLAN+L F NG  KIADFGLAK    K AAETEQ+FEWRGTP+ MS
Sbjct: 123 LRHVHGNGFVHCDLKLANVLNFGNGDVKIADFGLAKVAGKKTAAETEQRFEWRGTPMSMS 182

Query: 181 PESVNSGVYEPPCDIWALGCAVVEMVAGKPAWNCKPETDIFGLMMRIGAGDEVPDPPENL 240
           PE+V  G YEPPCDIWALGCAVVEMVAGKPAWN +PETDIFGLMMRIG GDEVP+ PENL
Sbjct: 183 PETVTGGEYEPPCDIWALGCAVVEMVAGKPAWNVRPETDIFGLMMRIGVGDEVPELPENL 242

Query: 241 SDDGKDFLRKCFIKDPTKRWTAEMLLNHPFVVGGGDTVTLKETEPPAASPRGPFDFPEFG 300
           SD+G+DFLR+CF+KDP KRWTAEMLLNHPFV G   TVTLKE EPP  SP GPFDFPE  
Sbjct: 243 SDEGRDFLRRCFVKDPRKRWTAEMLLNHPFVAG--ITVTLKEAEPPMESPTGPFDFPE-- 302

Query: 301 SLPLSSDERCFTPAIDSGLDFAPAMSTIRRLVTEKPLDWSVSDSWVTVR 343
           SLP  SD+        S       MS +RRLVTEKPLDWSVSDSWVTVR
Sbjct: 303 SLPTCSDQPSDDSYFCSSTVSPETMSRLRRLVTEKPLDWSVSDSWVTVR 347

[Close]

BLAST of MC02g_new0218 vs. ExPASy TrEMBL
Match: A0A6J1G8M2 (mitogen-activated protein kinase kinase kinase 18-like OS=Cucurbita moschata OX=3662 GN=LOC111451851 PE=3 SV=1)

HSP 1 Score: 523 bits (1346), Expect = 1.33e-184
Identity = 246/349 (70.49%), Postives = 288/349 (82.52%), Query Frame = 0


Query: 1   MEWIRGDQLGSGNFATINLAILTKEFDQFPPLMAVKTSPASS--SVSLKNEKQIFDQIGS 60
           MEW+RGD+LG GNF TI+LAILTK FDQFPPLMAVK+S + S  SVSLKNEK++ D+IG+
Sbjct: 1   MEWVRGDELGRGNFGTISLAILTKGFDQFPPLMAVKSSVSVSVSSVSLKNEKKVLDRIGN 60

Query: 61  CPQILTCFGDGYTIERDGQKHYNLFLEYANGGSLADKVKAHGGGLPEFDVQSYMAAVLNG 120
           CPQI+TCFGDG+++ERDG+K+YNLFLEYANGGSLAD VK HGGGL EFDV+ Y  A+L G
Sbjct: 61  CPQIVTCFGDGFSVERDGEKYYNLFLEYANGGSLADTVKLHGGGLLEFDVRRYTRAILCG 120

Query: 121 LRFIHENGFVHCDIKLANILTFSNGGAKIADFGLAKKAAA----ETEQKFEWRGTPLYMS 180
           LR +H NGFVHCD+KL+N+L F NG  KIADFGLAK+AAA    ETE+KFEWRGTP+YMS
Sbjct: 121 LRHVHGNGFVHCDLKLSNVLVFGNGEVKIADFGLAKEAAAAETEETEEKFEWRGTPMYMS 180

Query: 181 PESVNSGVYEPPCDIWALGCAVVEMVAGKPAWNCKPETDIFGLMMRIGAGDEVPDPPENL 240
           PE+VN G Y PPCD+WALGCAVVEMV GKPAW  KPETDIFGLMM IG GDEVP+ PENL
Sbjct: 181 PETVNGGKYAPPCDVWALGCAVVEMVGGKPAWRVKPETDIFGLMMMIGVGDEVPELPENL 240

Query: 241 SDDGKDFLRKCFIKDPTKRWTAEMLLNHPFVVGGGDTVTLKETEPPAASPRGPFDFPEFG 300
           S++GKDFLR+CF+KDP KRWTAEMLLNHPFV G G+TVTLKE+     SP GPFDFPEF 
Sbjct: 241 SEEGKDFLRRCFVKDPRKRWTAEMLLNHPFVAGAGETVTLKESNLTTESPTGPFDFPEFI 300

Query: 301 SLPLSSDERCFTPAIDSGLDFAPAMSTIRRLVTEKPLDWSVSDSWVTVR 343
               SS++RC   ++      A  M+ +RR+VTEKPLDWS ++SWVTVR
Sbjct: 301 ---CSSNQRCEEMSLCCSSSTAEMMNRLRRVVTEKPLDWSFTESWVTVR 346

[Close]

BLAST of MC02g_new0218 vs. ExPASy TrEMBL
Match: A0A6J1L7G1 (mitogen-activated protein kinase kinase kinase 18-like OS=Cucurbita maxima OX=3661 GN=LOC111499874 PE=3 SV=1)

HSP 1 Score: 515 bits (1326), Expect = 1.47e-181
Identity = 243/349 (69.63%), Postives = 285/349 (81.66%), Query Frame = 0


Query: 1   MEWIRGDQLGSGNFATINLAILTKEFDQFPPLMAVKTSPASS--SVSLKNEKQIFDQIGS 60
           MEW+RGD+LG GNF TI+LA LTK FDQFPPLMAVK+S + S  S SLKNEK++ D+IG+
Sbjct: 1   MEWVRGDELGRGNFGTISLARLTKGFDQFPPLMAVKSSVSVSVSSASLKNEKKVLDRIGN 60

Query: 61  CPQILTCFGDGYTIERDGQKHYNLFLEYANGGSLADKVKAHGGGLPEFDVQSYMAAVLNG 120
           CPQI+TC+GDG+++ERDG+K+YNLFLEYANGGSLAD VK HGGGL EFDV+ Y  A+L G
Sbjct: 61  CPQIVTCYGDGFSVERDGEKYYNLFLEYANGGSLADTVKLHGGGLLEFDVRRYTRAILCG 120

Query: 121 LRFIHENGFVHCDIKLANILTFSNGGAKIADFGLAKKAAA----ETEQKFEWRGTPLYMS 180
           LR +H NGFVHCD+KL+N+L F NG  KIADFGLAK+AAA    ETE+ FEWRGTP+YMS
Sbjct: 121 LRHVHGNGFVHCDLKLSNVLVFGNGEVKIADFGLAKEAAAAETEETEENFEWRGTPMYMS 180

Query: 181 PESVNSGVYEPPCDIWALGCAVVEMVAGKPAWNCKPETDIFGLMMRIGAGDEVPDPPENL 240
           PE+VN G Y PPCD+WALGCAVVEMV GKPAW  KPETDIFGLMM IG GDEVP+ PENL
Sbjct: 181 PETVNGGKYAPPCDVWALGCAVVEMVRGKPAWRVKPETDIFGLMMMIGIGDEVPELPENL 240

Query: 241 SDDGKDFLRKCFIKDPTKRWTAEMLLNHPFVVGGGDTVTLKETEPPAASPRGPFDFPEFG 300
           S++GK+FLR+CF+KDP KRWTAEMLLNHPFV G G+TVTLKE+     SP GPFDFPEF 
Sbjct: 241 SEEGKNFLRRCFVKDPRKRWTAEMLLNHPFVAGAGETVTLKESNLSTESPTGPFDFPEFV 300

Query: 301 SLPLSSDERCFTPAIDSGLDFAPAMSTIRRLVTEKPLDWSVSDSWVTVR 343
               SS++RC    +      A  M+ +RRLVTEKPLDWSV++SWVTVR
Sbjct: 301 ---CSSNQRCDEMNLCCSSSSAEMMNRLRRLVTEKPLDWSVTESWVTVR 346

[Close]

BLAST of MC02g_new0218 vs. ExPASy TrEMBL
Match: A0A0A0LMI4 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G278170 PE=3 SV=1)

HSP 1 Score: 506 bits (1304), Expect = 3.16e-178
Identity = 245/349 (70.20%), Postives = 279/349 (79.94%), Query Frame = 0


Query: 1   MEWIRGDQLGSGNFATINLAILTKEFDQFPPLMAVKTSPAS-SSVS-LKNEKQIFDQIGS 60
           MEWIRGDQLG GNFATINLA LTK FDQFPPLMAVK+S +S SSVS LKNEKQI D+IG 
Sbjct: 1   MEWIRGDQLGRGNFATINLAKLTKGFDQFPPLMAVKSSVSSLSSVSSLKNEKQILDRIGV 60

Query: 61  CPQILTCFGDGYTIERDGQKHYNLFLEYANGGSLADKVKAHGGGLPEFDVQSYMAAVLNG 120
           CPQI+TC+GDG+++E+DG K YNLFLEYANGGSLAD ++ HGGGL EFDV+ Y  A+L G
Sbjct: 61  CPQIITCYGDGFSVEKDGDKCYNLFLEYANGGSLADALRIHGGGLSEFDVRRYTRAILCG 120

Query: 121 LRFIHENGFVHCDIKLANILTFSNGGAKIADFGLAKKA----AAETEQKFEWRGTPLYMS 180
           L+ +H NGFVHCD+KL+N+L F NG  KIADFGLAK A    A ETE++FEWRGTP+YMS
Sbjct: 121 LQHVHGNGFVHCDLKLSNVLIFGNGEVKIADFGLAKSAGKFAAVETEERFEWRGTPMYMS 180

Query: 181 PESVNSGVYEPPCDIWALGCAVVEMVAGKPAWNCKPETDIFGLMMRIGAGDEVPDPPENL 240
           PE VN G YE PCDIWALGCAVVEMV GKPAW   PETD+FGLMMRIG GDEVP+ PENL
Sbjct: 181 PEIVNDGEYESPCDIWALGCAVVEMVVGKPAWRVGPETDMFGLMMRIGVGDEVPEVPENL 240

Query: 241 SDDGKDFLRKCFIKDPTKRWTAEMLLNHPFVVGGGDTVTLKETEPPAASPRGPFDFPEFG 300
           S +GKDF+R+CF+KDP+KRWTAE+LLNHPFV G GDTVTLKE E    SP GPFDFPEF 
Sbjct: 241 SAEGKDFIRRCFVKDPSKRWTAEILLNHPFVAGAGDTVTLKEVELATESPTGPFDFPEFV 300

Query: 301 SLPLSSDERCFTPAIDSGLDFAPAMSTIRRLVTEKPLDWSVSDSWVTVR 343
                SDE  F  +  S       +S +R+L+T KPLDWSV DSWVTVR
Sbjct: 301 CSGQGSDEWSFCSSSSS----PEVLSRVRQLMTGKPLDWSVMDSWVTVR 345

[Close]

BLAST of MC02g_new0218 vs. TAIR 10
Match: AT4G36950.1 (mitogen-activated protein kinase kinase kinase 21 )

HSP 1 Score: 339.3 bits (869), Expect = 3.5e-93
Identity = 174/350 (49.71%), Postives = 235/350 (67.14%), Query Frame = 0


Query: 1   MEWIRGDQLGSGNFATINLAILTKEFDQ-FPPLMAVKTSPASSSVSLKNEKQIFDQIGSC 60
           MEWIR + +G G+F+T++LA  +    + FP LMAVK+S    S +L+NE+ + D +G C
Sbjct: 1   MEWIRRETIGHGSFSTVSLATTSGSSSKAFPSLMAVKSSGVVCSAALRNERDVLDDLGDC 60

Query: 61  PQILTCFGDGYTIERDGQKHYNLFLEYANGGSLADKVKAHGGGLPEFDVQSYMAAVLNGL 120
            +I+ CFG+G T+E +G++ YNLFLEYA+GGSLAD++K+ G  LPEF+V+ +  +++ GL
Sbjct: 61  SEIVRCFGEGRTVE-NGEEIYNLFLEYASGGSLADRIKSSGEALPEFEVRRFTRSIVKGL 120

Query: 121 RFIHENGFVHCDIKLANILTFSNGGAKIADFGLAKKAAAETEQKFEWRGTPLYMSPESVN 180
             IH NGF HCDIKL N+L F +G  KI+DFGLAK+ + E     E RGTPLYM+PESVN
Sbjct: 121 CHIHGNGFTHCDIKLENVLVFGDGDVKISDFGLAKRRSGEV--CVEIRGTPLYMAPESVN 180

Query: 181 SGVYEPPCDIWALGCAVVEMVAGKPAWNCKPE---TDIFGLMMRIGAGDEVPDPPENLSD 240
            G +E P DIWALGC+VVEM +GK AW C  +    ++  L++RIG+GDEVP  P  LS+
Sbjct: 181 HGEFESPADIWALGCSVVEMSSGKTAW-CLEDGVMNNVMSLLVRIGSGDEVPRIPVELSE 240

Query: 241 DGKDFLRKCFIKDPTKRWTAEMLLNHPFVVGGGDTVTLKETEPPAASPRGPFDFPEFGSL 300
           +GKDF+ KCF+K+  +RWTAEMLL+HPF+    D  + +E E  + SPR PFDFP + S+
Sbjct: 241 EGKDFVSKCFVKNAAERWTAEMLLDHPFL--AVDDESGEEDEACSVSPRNPFDFPGWNSV 300

Query: 301 PLSSDERCFTPAIDS---GLDFAPAMSTIRRLVTEKPLDWSVSDSWVTVR 344
                    +P  DS   G         I  LV+EK  DWSVS  WV VR
Sbjct: 301 Q--------SPVNDSVMFGSLVGSPEERISGLVSEKVPDWSVSCDWVNVR 336

[Close]

BLAST of MC02g_new0218 vs. TAIR 10
Match: AT5G67080.1 (mitogen-activated protein kinase kinase kinase 19 )

HSP 1 Score: 332.8 bits (852), Expect = 3.3e-91
Identity = 176/357 (49.30%), Postives = 230/357 (64.43%), Query Frame = 0


Query: 1   MEWIRGDQLGSGNFATINLAILT-KEFDQFPPLMAVKTSPASSSVSLKNEKQIFDQIG-S 60
           MEWIRG+ +G G F+T++LA  +  +  +FPPLMAVK++ +  + SL NEK + D +G  
Sbjct: 1   MEWIRGETIGYGTFSTVSLATRSNNDSGEFPPLMAVKSADSYGAASLANEKSVLDNLGDD 60

Query: 61  CPQILTCFGDGYTIERDGQKHYNLFLEYANGGSLADKVKAHGG-GLPEFDVQSYMAAVLN 120
           C +I+ CFG+  T+E +G++ +NLFLEYA+ GSL   +K   G G+PE  V+ +  +VL 
Sbjct: 61  CNEIVRCFGEDRTVE-NGEEMHNLFLEYASRGSLESYLKKLAGEGVPESTVRRHTGSVLR 120

Query: 121 GLRFIHENGFVHCDIKLANILTFSNGGAKIADFGLAKKAAAETEQKF--EWRGTPLYMSP 180
           GLR IH NGF HCD+KL NIL F +G  KIADFGLAK+    T   +  + RGTPLYM+P
Sbjct: 121 GLRHIHANGFAHCDLKLGNILLFGDGAVKIADFGLAKRIGDLTALNYGVQIRGTPLYMAP 180

Query: 181 ESVNSGVYEPPCDIWALGCAVVEMVAGKPAWNCKPETDIFGLMMRIGAGDEVPDPPENLS 240
           ESVN   Y    D+WALGC VVEM +GK AW+ K  ++   L++RIG GDEVP  PE LS
Sbjct: 181 ESVNDNEYGSEGDVWALGCVVVEMFSGKTAWSLKEGSNFMSLLLRIGVGDEVPMIPEELS 240

Query: 241 DDGKDFLRKCFIKDPTKRWTAEMLLNHPFV---------VGGGDTVTLKETEPPAASPRG 300
           + G+DFL KCF+KDP KRWTAEMLLNHPFV         V   D V   +TE  + SPR 
Sbjct: 241 EQGRDFLSKCFVKDPKKRWTAEMLLNHPFVTVDVDHDVLVKEEDFVVNMKTEDVSTSPRC 300

Query: 301 PFDFPEFGSLPLSSDERCFTPAIDSGLDFAPAMSTIRRLVTEKPLDWSVSDSWVTVR 344
           PF+FP++ S+   S        IDS          +  LVT+   DWSV++SWVTVR
Sbjct: 301 PFEFPDWVSVSSGSQ------TIDS------PDERVASLVTDMIPDWSVTNSWVTVR 344

[Close]

BLAST of MC02g_new0218 vs. TAIR 10
Match: AT3G50310.1 (mitogen-activated protein kinase kinase kinase 20 )

HSP 1 Score: 331.6 bits (849), Expect = 7.3e-91
Identity = 168/354 (47.46%), Postives = 226/354 (63.84%), Query Frame = 0


Query: 1   MEWIRGDQLGSGNFATINLAILTKEFDQFPPLMAVKTSPASSSVSLKNEKQIFDQIGSCP 60
           MEW+RG+ +G G F+T++ A  ++    FP L+AVK++ A  + SL NEK + D +G CP
Sbjct: 1   MEWVRGETIGFGTFSTVSTATKSRNSGDFPALIAVKSTDAYGAASLSNEKSVLDSLGDCP 60

Query: 61  QILTCFGDGYTIERDGQKHYNLFLEYANGGSLADKVKAHGG-GLPEFDVQSYMAAVLNGL 120
           +I+ C+G+  T+E +G++ +NL LEYA+ GSLA  +K  GG GLPE  V+ +  +VL GL
Sbjct: 61  EIIRCYGEDSTVE-NGEEMHNLLLEYASRGSLASYMKKLGGEGLPESTVRRHTGSVLRGL 120

Query: 121 RFIHENGFVHCDIKLANILTFSNGGAKIADFGLAKKAAAE---TEQKFEWRGTPLYMSPE 180
           R IH  GF HCDIKLANIL F++G  KIADFGLA +   +     +  E RGTPLYM+PE
Sbjct: 121 RHIHAKGFAHCDIKLANILLFNDGSVKIADFGLAMRVDGDLTALRKSVEIRGTPLYMAPE 180

Query: 181 SVNSGVYEPPCDIWALGCAVVEMVAGKPAWNCKPETDIFGLMMRIGAGDEVPDPPENLSD 240
            VN   Y    D+WALGCAVVEM +GK AW+ K  +    L++RIG GDE+P  PE LS+
Sbjct: 181 CVNDNEYGSAADVWALGCAVVEMFSGKTAWSVKEGSHFMSLLIRIGVGDELPKIPEMLSE 240

Query: 241 DGKDFLRKCFIKDPTKRWTAEMLLNHPFVV-------GGGDTVTLKETEPPAASPRGPFD 300
           +GKDFL KCF+KDP KRWTAEMLLNH FV             V +K+ +    SP+ PF+
Sbjct: 241 EGKDFLSKCFVKDPAKRWTAEMLLNHSFVTIDLEDDHRENFVVKVKDEDKVLMSPKCPFE 300

Query: 301 FPEFGSLPLSSDERCFTPAIDSGLDFAPAMSTIRRLVTEKPLDWSVSDSWVTVR 344
           F ++ S  L S+     P+ DS ++       +  LV+    DWSV  SW+TVR
Sbjct: 301 FDDWDSFTLDSN-----PSFDSPVE------RLGSLVSGSIPDWSVGGSWLTVR 342

[Close]

BLAST of MC02g_new0218 vs. TAIR 10
Match: AT4G26890.1 (mitogen-activated protein kinase kinase kinase 16 )

HSP 1 Score: 213.8 bits (543), Expect = 2.2e-55
Identity = 126/353 (35.69%), Postives = 183/353 (51.84%), Query Frame = 0


Query: 1   MEWIRGDQLGSGNFATINLAILTKEFDQFPPLMAVKTSPASSSVSLKNEKQIFDQIGSCP 60
           + W RG  +G G+ AT+++AI +        L AVK++  SSS  L+ E+ I   + S P
Sbjct: 3   INWTRGPIIGRGSTATVSIAISSS-----GELFAVKSADLSSSSLLQKEQSILSTLSS-P 62

Query: 61  QILTCFGDGYTIERDGQKHYNLFLEYANGGSLADKVKAHGGGLPEFDVQSYMAAVLNGLR 120
            ++   G G T E +G   YN+ +EY +GG+L D +K  GG LPE +++SY   +LNGL 
Sbjct: 63  HMVKYIGTGLTRESNGLV-YNILMEYVSGGNLHDLIKNSGGKLPEPEIRSYTRQILNGLV 122

Query: 121 FIHENGFVHCDIKLANILTFSNGGAKIADFGLAKKAAAETEQKFEWRGTPLYMSPESVNS 180
           ++HE G VHCD+K  N+L   NG  KIAD G AK        K E+ GTP +M+PE    
Sbjct: 123 YLHERGIVHCDLKSHNVLVEENGVLKIADMGCAKSV-----DKSEFSGTPAFMAPEVARG 182

Query: 181 GVYEPPCDIWALGCAVVEMVAGKPAWNCKPE-TDIFGLMMRIGAGDEVPDPPENLSDDGK 240
                P D+WALGC ++EM+ G   W   PE  D+   M +IG   E P  P  +SD  K
Sbjct: 183 EEQRFPADVWALGCTMIEMMTGSSPW---PELNDVVAAMYKIGFSGESPAIPAWISDKAK 242

Query: 241 DFLRKCFIKDPTKRWTAEMLLNHPFVVGGGDTVTLKETEPPAASPRGPFDFPEFGSLPLS 300
           DFL+ C  +D  +RWT E LL HPF+    ++ T    +   +SP    D   + S   S
Sbjct: 243 DFLKNCLKEDQKQRWTVEELLKHPFLDDDEESQTSDCLKNKTSSPSTVLDQRFWDSCESS 302

Query: 301 SDERCFTPAIDSGLDFAPAMST----IRRLVTEK---PLDWSVSD--SWVTVR 344
                     D   +++ ++ +    I +L  ++    LDW   D   W+ VR
Sbjct: 303 KSHLVSIDHEDPFAEYSESLDSPADRIEKLAGDEFSSLLDWDTEDDGGWIQVR 340

[Close]

BLAST of MC02g_new0218 vs. TAIR 10
Match: AT5G55090.1 (mitogen-activated protein kinase kinase kinase 15 )

HSP 1 Score: 207.6 bits (527), Expect = 1.6e-53
Identity = 117/266 (43.98%), Postives = 153/266 (57.52%), Query Frame = 0


Query: 3   WIRGDQLGSGNFATINLAILTKEFDQFPPLMAVKTSPASSSVSLKNEKQIFDQIGSCPQI 62
           WIRG  +G G+ AT++L I T   D F    AVK++  SSS  L+ E+ I  ++ S P I
Sbjct: 6   WIRGPIIGRGSTATVSLGI-TNSGDFF----AVKSAEFSSSAFLQREQSILSKLSS-PYI 65

Query: 63  LTCFGDGYTIERDGQKHYNLFLEYANGGSLADKVKAHGGGLPEFDVQSYMAAVLNGLRFI 122
           +   G   T E D +  YNL +EY +GGSL D +K  GG LPE  ++SY   +L GL ++
Sbjct: 66  VKYIGSNVTKEND-KLMYNLLMEYVSGGSLHDLIKNSGGKLPEPLIRSYTRQILKGLMYL 125

Query: 123 HENGFVHCDIKLANILTFSNGG--AKIADFGLAKKAAAETEQKFEWRGTPLYMSPESVNS 182
           H+ G VHCD+K  N++    GG  AKI D G AK    E  +  E+ GTP +MSPE    
Sbjct: 126 HDQGIVHCDVKSQNVMI---GGEIAKIVDLGCAK--TVEENENLEFSGTPAFMSPEVARG 185

Query: 183 GVYEPPCDIWALGCAVVEMVAGKPAWNCKPE-TDIFGLMMRIGAGDEVPDPPENLSDDGK 242
                P D+WALGC V+EM  G   W   PE  D+   + +IG   E P  P  LS+ G+
Sbjct: 186 EEQSFPADVWALGCTVIEMATGSSPW---PELNDVVAAIYKIGFTGESPVIPVWLSEKGQ 245

Query: 243 DFLRKCFIKDPTKRWTAEMLLNHPFV 266
           DFLRKC  KDP +RWT E LL HPF+
Sbjct: 246 DFLRKCLRKDPKQRWTVEELLQHPFL 256

The following BLAST results are available for this feature:

BLAST of MC02g_new0218 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SND6	1.0e-89	47.46	Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=370...	[more]
O80888	4.8e-47	38.72	Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=370...	[more]
Q9ZVP5	4.1e-46	39.48	Mitogen-activated protein kinase kinase kinase 18 OS=Arabidopsis thaliana OX=370...	[more]
F4HRJ4	7.7e-37	33.22	Mitogen-activated protein kinase kinase kinase 3 OS=Arabidopsis thaliana OX=3702...	[more]
O22042	2.2e-36	32.85	Mitogen-activated protein kinase kinase kinase 3 OS=Arabidopsis thaliana OX=3702...	[more]

BLAST of MC02g_new0218 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022149525.1	1.18e-262	100.00	mitogen-activated protein kinase kinase kinase 17-like [Momordica charantia]	[more]
XP_022149480.1	1.90e-189	74.21	mitogen-activated protein kinase kinase kinase 18-like [Momordica charantia]	[more]
XP_022948206.1	2.74e-184	70.49	mitogen-activated protein kinase kinase kinase 18-like [Cucurbita moschata]	[more]
KAG6605205.1	7.85e-184	70.49	Mitogen-activated protein kinase kinase kinase 20, partial [Cucurbita argyrosper...	[more]
XP_023007353.1	3.04e-181	69.63	mitogen-activated protein kinase kinase kinase 18-like [Cucurbita maxima]	[more]

BLAST of MC02g_new0218 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D5Z2	5.73e-263	100.00	mitogen-activated protein kinase kinase kinase 17-like OS=Momordica charantia OX...	[more]
A0A6J1D5U5	9.19e-190	74.21	mitogen-activated protein kinase kinase kinase 18-like OS=Momordica charantia OX...	[more]
A0A6J1G8M2	1.33e-184	70.49	mitogen-activated protein kinase kinase kinase 18-like OS=Cucurbita moschata OX=...	[more]
A0A6J1L7G1	1.47e-181	69.63	mitogen-activated protein kinase kinase kinase 18-like OS=Cucurbita maxima OX=36...	[more]
A0A0A0LMI4	3.16e-178	70.20	Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G278...	[more]

BLAST of MC02g_new0218 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G36950.1	3.5e-93	49.71	mitogen-activated protein kinase kinase kinase 21	[more]
AT5G67080.1	3.3e-91	49.30	mitogen-activated protein kinase kinase kinase 19	[more]
AT3G50310.1	7.3e-91	47.46	mitogen-activated protein kinase kinase kinase 20	[more]
AT4G26890.1	2.2e-55	35.69	mitogen-activated protein kinase kinase kinase 16	[more]
AT5G55090.1	1.6e-53	43.98	mitogen-activated protein kinase kinase kinase 15	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000719	Protein kinase domain	SMART	SM00220	serkin_6	coord: 3..265 e-value: 8.1E-65 score: 231.3
IPR000719	Protein kinase domain	PFAM	PF00069	Pkinase	coord: 6..265 e-value: 8.1E-53 score: 179.5
IPR000719	Protein kinase domain	PROSITE	PS50011	PROTEIN_KINASE_DOM	coord: 3..265 score: 36.852299
None	No IPR available	GENE3D	1.10.510.10	Transferase(Phosphotransferase) domain 1	coord: 94..283 e-value: 4.7E-51 score: 175.5
None	No IPR available	GENE3D	3.30.200.20	Phosphorylase Kinase; domain 1	coord: 1..93 e-value: 5.4E-7 score: 31.8
None	No IPR available	PANTHER	PTHR48011:SF18	MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 20	coord: 1..300
None	No IPR available	PANTHER	PTHR48011	CCR4-NOT TRANSCRIPTIONAL COMPLEX SUBUNIT CAF120-RELATED	coord: 1..300
None	No IPR available	CDD	cd06606	STKc_MAPKKK	coord: 2..265 e-value: 2.53371E-106 score: 309.836
IPR008271	Serine/threonine-protein kinase, active site	PROSITE	PS00108	PROTEIN_KINASE_ST	coord: 127..139
IPR017441	Protein kinase, ATP binding site	PROSITE	PS00107	PROTEIN_KINASE_ATP	coord: 9..36
IPR011009	Protein kinase-like domain superfamily	SUPERFAMILY	56112	Protein kinase-like (PK-like)	coord: 3..293

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC02g_new0218.1	MC02g_new0218.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006468	protein phosphorylation
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004674	protein serine/threonine kinase activity
molecular_function	GO:0004672	protein kinase activity