MC02g_new0216 (gene) Bitter gourd (Dali-11) v1

BLAST of MC02g_new0216 vs. NCBI nr
Match: XP_022149515.1 (uncharacterized protein LOC111017927 [Momordica charantia])

HSP 1 Score: 435 bits (1119), Expect = 7.55e-153
Identity = 220/276 (79.71%), Postives = 220/276 (79.71%), Query Frame = 0

Query: 1   MKKNLPFYSASLLLYQLALVMKRWRWNESLIRNSFLEEEADIILNIPLSTCNQHDEVIWG 60
           MKKNLPFYSASLLLYQLALVMKRWRWNESLIRNSFLEEEADIILNIPLSTCNQHDEVIWG
Sbjct: 1   MKKNLPFYSASLLLYQLALVMKRWRWNESLIRNSFLEEEADIILNIPLSTCNQHDEVIWG 60

Query: 61  PDKKDKFSVKSTYRLGVHLASADEVQTSNSEEEAKKWKKLWRTLVPTKVKICCWRIYNDI 120
           PDKKDKFSVKSTYRLGVHLASADEVQTSNSEEEAKKWKKLWRTLVPTKVKICCWRIYNDI
Sbjct: 61  PDKKDKFSVKSTYRLGVHLASADEVQTSNSEEEAKKWKKLWRTLVPTKVKICCWRIYNDI 120

Query: 121 ISTIAILNKKGIVIRQGCSFCKKEEEESSTHIFWNCRFVRDIWALYLNSNMCDFWLNRHV 180
           ISTIAILNKKGIVIRQGCSFCKKEEEESSTHIFWNCR                       
Sbjct: 121 ISTIAILNKKGIVIRQGCSFCKKEEEESSTHIFWNCR----------------------- 180

Query: 181 LSSKDFCDPMWSRASGDELQKSQLILWKLWGYRNMIYHSKENPNIQTLIEEIEIVLRNHN 240
                                            NMIYHSKENPNIQTLIEEIEIVLRNHN
Sbjct: 181 ---------------------------------NMIYHSKENPNIQTLIEEIEIVLRNHN 220

Query: 241 LKGETNLVKRVRRSTIEGSSWTTPPHHRWTLPGWTP 276
           LKGETNLVKRVRRSTIEGSSWTTPPHHRWTLPGWTP
Sbjct: 241 LKGETNLVKRVRRSTIEGSSWTTPPHHRWTLPGWTP 220

BLAST of MC02g_new0216 vs. NCBI nr
Match: TXG65555.1 (hypothetical protein EZV62_006830 [Acer yangbiense])

HSP 1 Score: 118 bits (296), Expect = 3.76e-28
Identity = 72/208 (34.62%), Postives = 107/208 (51.44%), Query Frame = 0

Query: 19  LVMKRWRWNESLIRNSFLEEEADIILNIPLSTCNQHDEVIWGPDKKDKFSVKSTYRLGVH 78
           L+ ++ RW++ L+R+ FL +E+++IL+IPLS   + D  +W  DKK KFSVKS Y++   
Sbjct: 5   LMEQQGRWDQRLVRSCFLGDESEVILSIPLSINPREDAYLWHFDKKGKFSVKSAYKVA-- 64

Query: 79  LASADEVQTSNSEEEAKKWKKLWRTLVPTKVKICCWRIYNDIISTIAILNKKGIVIRQGC 138
           LA     Q S S      WKKLW   +P+K++  CW+    I+ T  +L KKG+V+   C
Sbjct: 65  LADQLSDQASCSYGPLAWWKKLWSLELPSKIRFFCWKANKVILPTKLLLCKKGLVVSDLC 124

Query: 139 SFCKKEEEESSTHIFWNCRFVRDIWALYLNSNMCDFWLNRHVLSSKDFCDP-MWSRASG- 198
            FC  +E ES  H  W C+ V   W  +        + + + L    F D  +W  + G 
Sbjct: 125 PFCG-DEPESVDHALWRCKRVVPHWGTW------SLFPDINKLRGGCFIDRVLWLSSMGN 184

Query: 199 -DELQKSQLILWKLWGYRNMIYHSKENP 223
            D L    +  W LW  RN + H    P
Sbjct: 185 KDVLLHFLVTAWMLWFRRNRLVHGGTVP 203

BLAST of MC02g_new0216 vs. NCBI nr
Match: KAE8788950.1 (hypothetical protein D1007_36964 [Hordeum vulgare])

HSP 1 Score: 121 bits (304), Expect = 7.42e-28
Identity = 83/262 (31.68%), Postives = 116/262 (44.27%), Query Frame = 0

Query: 25  RWNESLIRNSFLEEEADIILNIPLSTCNQHDEVIWGPDKKDKFSVKSTYRLGVHLASADE 84
           RWN  LI ++FL  +AD+ILNIPL T +  D   W  DKK  FSVKS YR+ +      E
Sbjct: 12  RWNRELITSTFLPIDADVILNIPLRTRHYEDYWSWSQDKKGLFSVKSCYRMLITTKLRRE 71

Query: 85  VQTSNS------EEEAKKWKKLWRTLVPTKVKICCWRIYNDIISTIAILNKKGIVIRQGC 144
                +        E K W +LWR  VP+K+ I  WR+    + T  I + + +   + C
Sbjct: 72  AWLEGNGGPADVTRERKCWDELWRIQVPSKINISLWRLSQTSLPTADIRHHRNMAPSRAC 131

Query: 145 SFCKKEEEESSTHIFWNCRFVRDIWALYLNSNMCDFWLNRHVLSSKDFCDPMWSRASGDE 204
           + C  E+  S  H    C   R +W L+ NS + +  L      +K +   M S  S  E
Sbjct: 132 AICGAED--SWIHSLLTCTMARCVWVLH-NSELVEHMLANAEPDAKSWLFSMRSSVSATE 191

Query: 205 LQKSQLILWKLWGYRNMIYHSKENPNIQTLIEEIEIVLRNHNLKGETNLVKRVRRSTIEG 264
           + K  + LW +W  R  + H  E+ N    +  +   +       E  L K   RST   
Sbjct: 192 MTKITVTLWAIWFARRKLIHEGEHQNPGATLAFVNRYI------AELELNKPASRST--- 251

Query: 265 SSWTTPPH---HRWTLP--GWT 275
               TP H    RW  P  GWT
Sbjct: 252 ---PTPVHSNTRRWIKPPAGWT 258

BLAST of MC02g_new0216 vs. NCBI nr
Match: XP_040993121.1 (uncharacterized protein LOC121239848 [Juglans microcarpa x Juglans regia])

HSP 1 Score: 115 bits (287), Expect = 6.51e-27
Identity = 66/210 (31.43%), Postives = 105/210 (50.00%), Query Frame = 0

Query: 21  MKRWRWNESLIRNSFLEEEADIILNIPLSTCNQHDEVIWGPDKKDKFSVKSTYRLGVHLA 80
           M   RW E L+R  F EEEA++IL+IP+S     D +IW P+K  K+S+KS Y + +   
Sbjct: 1   MNSRRWKEELVRMIFEEEEANLILSIPISRGRAMDRLIWEPEKNGKYSIKSAYLMELRER 60

Query: 81  SADEVQTSNSEEEAKKWKKLWRTLVPTKVKICCWRIYNDIISTIAILNKKGIVIRQGCSF 140
                ++   ++EA+ WK+LWR  VP  V    W++ N+I+ T   L KK IV    C  
Sbjct: 61  ERINGESFEVDKEAELWKQLWRLEVPGTVNQFLWKVANNILPTNLNLWKKSIVKESNCPI 120

Query: 141 CKKEEEESSTHIFWNCRFVRDIWALYLNSNMCDFWLNRHVLSSKDFCDPMWSRASGDELQ 200
            +  + ES  H  WNC    D+WA    S +  +W      S       M+ + + + ++
Sbjct: 121 -RLMDVESVMHAIWNCPAASDVWAKE-GSPVKKWWSGEQ--SFTQLWTDMYRKLTKNNIE 180

Query: 201 KSQLILWKLWGYRNMIYHSKENPNIQTLIE 230
           K  +I+  +W  RN      E  N + +++
Sbjct: 181 KMVMIMKNIWYRRNKFVFETEFLNPKQVVQ 206

BLAST of MC02g_new0216 vs. NCBI nr
Match: KAE8785787.1 (hypothetical protein D1007_40474 [Hordeum vulgare])

HSP 1 Score: 112 bits (279), Expect = 3.29e-25
Identity = 64/206 (31.07%), Postives = 101/206 (49.03%), Query Frame = 0

Query: 25  RWNESLIRNSFLEEEADIILNIPLSTCNQHDEVIWGPDKKDKFSVKSTYRLGVHLASADE 84
           RWN  LI ++FL  EA++ILNIPLST +  D   W  +KK  F+V+S YR+ + +    E
Sbjct: 12  RWNRDLITSTFLPIEAEVILNIPLSTRHYEDYWSWSQEKKGVFTVRSCYRMLITMKLRRE 71

Query: 85  VQTSNS------EEEAKKWKKLWRTLVPTKVKICCWRIYNDIISTIAILNKKGIVIRQGC 144
              + +        E K W +LWR  VP+K+KI  WR+    + T  + + + +   + C
Sbjct: 72  AWLAGNGGPADVTREKKCWDELWRIQVPSKIKIFLWRLAQTSLPTADVRHHRNMAPNRAC 131

Query: 145 SFCKKEEEESSTHIFWNCRFVRDIWALYLNSNMCDFWLNRHVLSSKDFCDPMWSRASGDE 204
           + C  E+  S  H    C   R +W L+ N  + +  L      +K +   M S  S  E
Sbjct: 132 AVCGAED--SWIHSLLTCTMARCVWVLH-NPELVEHMLVTAEPDAKSWLFSMRSSVSETE 191

Query: 205 LQKSQLILWKLWGYRNMIYHSKENPN 224
           + K  + LW +W  R  + H  E+ +
Sbjct: 192 MTKITVTLWAIWYGRRKLIHEGEHQS 214

BLAST of MC02g_new0216 vs. ExPASy TrEMBL
Match: A0A6J1D5Y4 (uncharacterized protein LOC111017927 OS=Momordica charantia OX=3673 GN=LOC111017927 PE=4 SV=1)

HSP 1 Score: 435 bits (1119), Expect = 3.66e-153
Identity = 220/276 (79.71%), Postives = 220/276 (79.71%), Query Frame = 0

Query: 1   MKKNLPFYSASLLLYQLALVMKRWRWNESLIRNSFLEEEADIILNIPLSTCNQHDEVIWG 60
           MKKNLPFYSASLLLYQLALVMKRWRWNESLIRNSFLEEEADIILNIPLSTCNQHDEVIWG
Sbjct: 1   MKKNLPFYSASLLLYQLALVMKRWRWNESLIRNSFLEEEADIILNIPLSTCNQHDEVIWG 60

Query: 61  PDKKDKFSVKSTYRLGVHLASADEVQTSNSEEEAKKWKKLWRTLVPTKVKICCWRIYNDI 120
           PDKKDKFSVKSTYRLGVHLASADEVQTSNSEEEAKKWKKLWRTLVPTKVKICCWRIYNDI
Sbjct: 61  PDKKDKFSVKSTYRLGVHLASADEVQTSNSEEEAKKWKKLWRTLVPTKVKICCWRIYNDI 120

Query: 121 ISTIAILNKKGIVIRQGCSFCKKEEEESSTHIFWNCRFVRDIWALYLNSNMCDFWLNRHV 180
           ISTIAILNKKGIVIRQGCSFCKKEEEESSTHIFWNCR                       
Sbjct: 121 ISTIAILNKKGIVIRQGCSFCKKEEEESSTHIFWNCR----------------------- 180

Query: 181 LSSKDFCDPMWSRASGDELQKSQLILWKLWGYRNMIYHSKENPNIQTLIEEIEIVLRNHN 240
                                            NMIYHSKENPNIQTLIEEIEIVLRNHN
Sbjct: 181 ---------------------------------NMIYHSKENPNIQTLIEEIEIVLRNHN 220

Query: 241 LKGETNLVKRVRRSTIEGSSWTTPPHHRWTLPGWTP 276
           LKGETNLVKRVRRSTIEGSSWTTPPHHRWTLPGWTP
Sbjct: 241 LKGETNLVKRVRRSTIEGSSWTTPPHHRWTLPGWTP 220

BLAST of MC02g_new0216 vs. ExPASy TrEMBL
Match: A0A5C7I8M1 (zf-RVT domain-containing protein OS=Acer yangbiense OX=1000413 GN=EZV62_006830 PE=4 SV=1)

HSP 1 Score: 118 bits (296), Expect = 1.82e-28
Identity = 72/208 (34.62%), Postives = 107/208 (51.44%), Query Frame = 0

Query: 19  LVMKRWRWNESLIRNSFLEEEADIILNIPLSTCNQHDEVIWGPDKKDKFSVKSTYRLGVH 78
           L+ ++ RW++ L+R+ FL +E+++IL+IPLS   + D  +W  DKK KFSVKS Y++   
Sbjct: 5   LMEQQGRWDQRLVRSCFLGDESEVILSIPLSINPREDAYLWHFDKKGKFSVKSAYKVA-- 64

Query: 79  LASADEVQTSNSEEEAKKWKKLWRTLVPTKVKICCWRIYNDIISTIAILNKKGIVIRQGC 138
           LA     Q S S      WKKLW   +P+K++  CW+    I+ T  +L KKG+V+   C
Sbjct: 65  LADQLSDQASCSYGPLAWWKKLWSLELPSKIRFFCWKANKVILPTKLLLCKKGLVVSDLC 124

Query: 139 SFCKKEEEESSTHIFWNCRFVRDIWALYLNSNMCDFWLNRHVLSSKDFCDP-MWSRASG- 198
            FC  +E ES  H  W C+ V   W  +        + + + L    F D  +W  + G 
Sbjct: 125 PFCG-DEPESVDHALWRCKRVVPHWGTW------SLFPDINKLRGGCFIDRVLWLSSMGN 184

Query: 199 -DELQKSQLILWKLWGYRNMIYHSKENP 223
            D L    +  W LW  RN + H    P
Sbjct: 185 KDVLLHFLVTAWMLWFRRNRLVHGGTVP 203

BLAST of MC02g_new0216 vs. ExPASy TrEMBL
Match: A0A803P9P5 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 124 bits (311), Expect = 3.34e-28
Identity = 80/274 (29.20%), Postives = 130/274 (47.45%), Query Frame = 0

Query: 3   KNLPFYSASLLLYQLALVMKRWRWNESLIRNSFLEEEADIILNIPLSTCNQHDEVIWGPD 62
           K L F + SL +    L+  + +WN SL++  FLE +   I +IPL+ C+Q D++IW  +
Sbjct: 426 KPLIFKNPSLSMKVSELITNQRQWNHSLLQQCFLESDVAKIQSIPLTLCDQSDQLIWNFE 485

Query: 63  KKDKFSVKSTYRLGVHLASADEVQTSNSEEEAKKWKKLWRTLVPTKVKICCWRIYNDIIS 122
               +SVKS Y L   L   +++  ++S    + WKK W   +P+K+KI  WR  +D + 
Sbjct: 486 NNGMYSVKSGYTLATRLE--EQLPAASSNNSLQWWKKFWSLTLPSKIKIFLWRAMHDCLP 545

Query: 123 TIAILNKKGIVIRQGCSFCKKEEEESSTHIFWNCRFVRDIWALYLNSNMCDFWLNRHVLS 182
              IL+ + I     C+ C +  E ++  +FW C+  R IW L  + ++ DF    H +S
Sbjct: 546 VADILHHRHISDSAICTLCHQAIESTTHALFW-CKRPRKIWQLS-SFSISDF--VTHNMS 605

Query: 183 SKDFCDPMWSRASGDELQKSQLILWKLWGYRNMIYH-SKENPNIQTLIEEIEIVLRNHNL 242
             D    M    S  ++++   ILW +W  RN   H SK  P   TL   ++ +    + 
Sbjct: 606 LMDVLQNMSQIWSSKQIEQFACILWSIWNERNKERHGSKTKPPEMTLFFAMDYIEEFQSA 665

Query: 243 KGETNLVKRVRRSTIEGSS----WTTPPHHRWTL 271
           +  T+       +T         W  PP  R  L
Sbjct: 666 RLNTSFADSATHATTRSQQQELPWMNPPSGRLKL 693

BLAST of MC02g_new0216 vs. ExPASy TrEMBL
Match: M5Y023 (zf-RVT domain-containing protein (Fragment) OS=Prunus persica OX=3760 GN=PRUPE_ppa022315mg PE=4 SV=1)

HSP 1 Score: 112 bits (281), Expect = 1.34e-25
Identity = 75/246 (30.49%), Postives = 120/246 (48.78%), Query Frame = 0

Query: 26  WNESLIRNSFLEEEADIILNIPLSTCNQHDEVIWGPDKKDKFSVKSTYRLGVHL-ASADE 85
           WN SL++N F  EE  +I +IPLS     D ++W  DKK  F+VKS Y +   L +S   
Sbjct: 20  WNASLLQNVFFPEEVMLIRSIPLSLRLTPDMLVWHYDKKGMFTVKSAYHVARSLHSSTGR 79

Query: 86  VQTSNSEEEAKKWKKLWRTLVPTKVKICCWRIYNDIISTIAILNKKGIVIRQGCSFCKKE 145
             +SNS+  A+ W  LW+ +VP +VK   WR+ + I+ T A L +K + + + C  C+  
Sbjct: 80  ASSSNSDAVARNWSLLWKAIVPARVKTFWWRVISGILPTKANLARKKVSLDEECMLCEGP 139

Query: 146 EEESSTHIFWNCRFVRDIWALYLNSNMCDFWLNRHVLSSKDFCDPMWSRASGDELQKSQL 205
            E SS HI  +C F    W    +S +     N    S KD+      + S  +     +
Sbjct: 140 VE-SSIHILRDCPFAICAWR---SSTLSSSRWNNGAHSPKDWVFRCAEQLSSQDFATFLM 199

Query: 206 ILWKLWGYRN-MIYHSKENPNIQTLIE---EIEIVLRNHNLKGETNLVKRVRRSTIEGSS 265
           + W +W  RN +++++K++   Q  +     ++  LR  N  G  +     R+  I+   
Sbjct: 200 VGWAIWEARNGLLWNNKKSRPEQVSLHASLRLQDFLRVSNCLGSQS-----RQGQIKQKM 255

BLAST of MC02g_new0216 vs. ExPASy TrEMBL
Match: A0A2I4DF11 (uncharacterized protein LOC108979499 OS=Juglans regia OX=51240 GN=LOC108979499 PE=4 SV=2)

HSP 1 Score: 116 bits (290), Expect = 2.24e-25
Identity = 74/247 (29.96%), Postives = 119/247 (48.18%), Query Frame = 0

Query: 26   WNESLIRNSFLEEEADIILNIPLSTCNQHDEVIWGPDKKDKFSVKSTYRLGVHLASADEV 85
            WN  L+ + F +EEADII+ IPLST N+ D++IW       F+VKS Y L   L    + 
Sbjct: 843  WNMELVEHLFCKEEADIIVQIPLSTSNRPDQLIWKGTSTGFFTVKSAYHLHQELIQDTKG 902

Query: 86   QTSNSEEEAKKWKKLWRTLVPTKVKICCWRIYNDIISTIAILNKKGIVIRQGCSFCKKEE 145
            Q S+S    + WK LW+  V    K+  WR  ++ + T + L K+ IV    C  C+ EE
Sbjct: 903  QPSSSHNSKEVWKTLWQLQVTPGEKVFLWRACHNALPTQSNLFKRKIVANPKCPICELEE 962

Query: 146  EESSTHIFWNCRFVRDIWALYLNSNMCDFWLNRHVLSSKDFCDPMWSRASG---DELQKS 205
            E +  H  W C   RD+W+       C   + +   S  DF +   + A G   + +Q+ 
Sbjct: 963  E-TVAHALWECESARDVWS------QCTKSIQKRHFSHMDFLEIFEAMAEGMTFECMQEF 1022

Query: 206  QLILWKLWGYRN-MIYHSK-ENPNIQTLIEEIEIVLRNHNLKGETNLVKRVRRSTIEGSS 265
             ++  ++W  RN  I++ +  +PN  T++        N  +  +  + K    ++I  +S
Sbjct: 1023 VMVAKQVWWRRNDFIFNQRFRHPNQVTMVAN-----ENIRMMKDLEMQKNESSTSINSNS 1077