Homology
BLAST of MC02g_new0213 vs. ExPASy Swiss-Prot
Match:
W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)
HSP 1 Score: 525.8 bits (1353), Expect = 5.5e-148
Identity = 256/498 (51.41%), Postives = 353/498 (70.88%), Query Frame = 0
Query: 7 YFSLSLLSILVVRFLLRSPSGRKLPPSPAFRLPIIGHLHLLGRPAQHAFKKLADKYGPVF 66
+ ++ LLSI + F + S R LPPSP LP+IGHLHL+G+ + L+ KYG VF
Sbjct: 10 FLAVVLLSISLKLFPV-SRRRRNLPPSPGLALPVIGHLHLIGKLLHRSLYDLSKKYGSVF 69
Query: 67 SLRFGSRLVVIITSPEAVKECFTKNDVVLAARPLLNSGKYMAYDNTIFAAASYGEHWRNL 126
SL+ G+RLV++++SP A +ECFTKND+V A RPL GKY+ Y+ T + YGEHWRNL
Sbjct: 70 SLQLGNRLVLVVSSPAAAEECFTKNDIVFANRPLFILGKYIGYNYTTMVGSPYGEHWRNL 129
Query: 127 RRIGSIEIYSPTRLNKFLDSRGDEVKRLLRRLGESNNSPPPASAGFSTVKLEPLFAELTF 186
RR+ ++EI+S LN+FL R DEVK+LL L +S+ F V+++ +EL+F
Sbjct: 130 RRLAAVEIFSAGSLNRFLSIREDEVKQLLLSLYQSSGQ------DFGKVEMKSKLSELSF 189
Query: 187 NVVMRMVAGKSYFGENVSNEEEAKQFRYIMKEIFMISEASSPGDFIPIWNWIDPTGYEKK 246
NV MRMVAGK YFG++V + +EAK FR ++ E+F + AS+PGDF+P WID YEKK
Sbjct: 190 NVTMRMVAGKRYFGQDV-DSDEAKLFRALIGEVFEHAGASNPGDFVPFLRWIDFKNYEKK 249
Query: 247 LKNIAKKVDIFLVNLIEEIRNRKGEGNTMVDHLLSMQVSEPQFFSDQIIKGLIWVIMLAG 306
+ I++++D FL LI E R K TM+DHLLS+Q S+P++++DQIIKG+I V++LAG
Sbjct: 250 VSKISQEMDAFLQRLIHESRINK-NNVTMIDHLLSLQESQPEYYTDQIIKGIIMVLLLAG 309
Query: 307 TDTQSGTLTWTMSHLLNNPEVLKKARAEIDTVVGRERLVEENDLSKMTYLQQVIAETLRL 366
TDT + T+ W MS LLN+PE L+KAR EI+T VG RL+EE DL K+TYL +I+ET RL
Sbjct: 310 TDTSAVTVEWAMSLLLNHPETLEKARTEIETQVGSNRLIEEQDLPKLTYLHNIISETFRL 369
Query: 367 NP-LPLLLPHCASEDCTIQGYHIPRDTMLLFNVWAIHRDPKLWDDALIFKPERHATLTIE 426
P P+L+PH +S+DC +QGY +P+ T+LL N WAIHRDP+ WD+ +FKPERH + +E
Sbjct: 370 CPAAPMLVPHESSDDCKVQGYDVPKGTILLVNAWAIHRDPEFWDEPTLFKPERHGGVELE 429
Query: 427 DQYKYLPFGLGRRACPGGGMANRLVGLALATMIQCFEWKRVSDELVDMTVGNGITASRAF 486
K +PFG+GRR+CPG G+A R+VGL L +IQCFEWKR+ + +DM G+G+T +A
Sbjct: 430 PS-KLMPFGMGRRSCPGSGLAQRVVGLTLGALIQCFEWKRIGEAKIDMAEGSGLTMPKAQ 489
Query: 487 PLEAMCKPWPDMERVLVE 504
PLEA+CKP + +V+ E
Sbjct: 490 PLEALCKPRNILHKVVSE 497
BLAST of MC02g_new0213 vs. ExPASy Swiss-Prot
Match:
Q6WNQ8 (Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1)
HSP 1 Score: 500.7 bits (1288), Expect = 1.9e-140
Identity = 251/490 (51.22%), Postives = 339/490 (69.18%), Query Frame = 0
Query: 6 LYFSLSLLSILVVRFLLRSPSGRKLPPSPAFRLPIIGHLHLLGRPAQHAFKKLADKYGPV 65
L SL L I + F S + LPP P LPIIG+LH L +P H F L+ KYG +
Sbjct: 8 LIISLFFLIITLKVFFNTSRKFKNLPPGPQC-LPIIGNLHQLKQPLHHTFHTLSQKYGQI 67
Query: 66 FSLRFGSRLVVIITSPEAVKECFTKNDVVLAARPLLNSGKYMAYDNTIFAAASYGEHWRN 125
FSL FGSRLVV+++S +ECFTKND+VLA RP +GKY+ Y+NT A + YG+HWRN
Sbjct: 68 FSLWFGSRLVVVVSSLTIAQECFTKNDIVLANRPHFLTGKYIGYNNTTVAQSPYGDHWRN 127
Query: 126 LRRIGSIEIYSPTRLNKFLDSRGDEVKRLLRRLGESNNSPPPASAGFSTVKLEPLFAELT 185
LRRI SIEI S RLN FL+ R DE+ RL+++L + + + GF+ V+L P+F+E+T
Sbjct: 128 LRRILSIEILSSHRLNSFLEIRRDEIMRLIQKLAQKSYN------GFTEVELRPMFSEMT 187
Query: 186 FNVVMRMVAGKSYFGE--NVSNEEEAKQFRYIMKEIFMISEASSPGDFIPIWNWIDPTGY 245
FN +MRMV+GK Y+G +VS+ EEA+ FR I+KE+ + A++ GDF+ W D G
Sbjct: 188 FNTIMRMVSGKRYYGNDCDVSDVEEARLFRGIIKEVVSLGGANNVGDFLGFLRWFDFDGL 247
Query: 246 EKKLKNIAKKVDIFLVNLIEEIRNRKGEGNTMVDHLLSMQVSEPQFFSDQIIKGLIWVIM 305
EK+LK I+K+ D FL LI+E R K NTM+DHLL+ Q S+P++++DQIIKGL+ V++
Sbjct: 248 EKRLKKISKRTDAFLQGLIDEHRFGKRNSNTMIDHLLTQQQSQPEYYTDQIIKGLMVVML 307
Query: 306 LAGTDTQSGTLTWTMSHLLNNPEVLKKARAEIDTVVGRERLVEENDLSKMTYLQQVIAET 365
LAGTDT S T+ W MS+LLN+PE++KKA+ E+DT +G +R V+E+D+SK+ YLQ ++ ET
Sbjct: 308 LAGTDTSSVTIEWAMSNLLNHPEIMKKAKNELDTHIGHDRQVDEHDISKLPYLQSIVYET 367
Query: 366 LRLN-PLPLLLPHCASEDCTIQGYHIPRDTMLLFNVWAIHRDPKLWDDALIFKPERHATL 425
LRL+ PLL+PH +SED ++ GY+IP++T+L+ N W IHRDP LW D FKPER
Sbjct: 368 LRLHAAAPLLVPHLSSEDFSLGGYNIPQNTILMVNAWVIHRDPNLWSDPTCFKPERFE-- 427
Query: 426 TIEDQYKYLPFGLGRRACPGGGMANRLVGLALATMIQCFEWKRVSDELVDMTVGNGITAS 485
+ K L FGLGRRACPG ++ R GL L +IQCFEWKR+ +E +DM GITA
Sbjct: 428 KEGEVNKLLSFGLGRRACPGENLSQRTEGLTLGLLIQCFEWKRIGEEKIDMVEAKGITAG 487
Query: 486 RAFPLEAMCK 493
+ L AMCK
Sbjct: 488 KKTSLNAMCK 488
BLAST of MC02g_new0213 vs. ExPASy Swiss-Prot
Match:
Q6WNQ9 (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 PE=1 SV=1)
HSP 1 Score: 499.2 bits (1284), Expect = 5.5e-140
Identity = 257/499 (51.50%), Postives = 343/499 (68.74%), Query Frame = 0
Query: 5 LLYFS-LSLLSILVVRFLLRSPSGR--KLPPSPAFRLPIIGHLHLLGRPAQHAFKKLADK 64
L Y+S LSL I+ ++ LL+ S R LPP P +PIIG+LH L P F L+
Sbjct: 2 LFYYSLLSLSFIITIKILLKITSRRLKNLPPGPP-TIPIIGNLHHLKHPLHRTFTTLSQT 61
Query: 65 YGPVFSLRFGSRLVVIITSPEAVKECFTKNDVVLAARPLLNSGKYMAYDNTIFAAASYGE 124
YG +FSL FGSRLVV+++SP ECFTKND++LA RP +GKY+ Y+ T +ASYG+
Sbjct: 62 YGDIFSLWFGSRLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGD 121
Query: 125 HWRNLRRIGSIEIYSPTRLNKFLDSRGDEVKRLLRRLGESNNSPPPASAGFSTVKLEPLF 184
HWRNLRRI +I++ S RLN FL R DE RL+++L + S GF+ V+L P
Sbjct: 122 HWRNLRRITTIDVLSNNRLNSFLGVRRDETNRLIQKLLKDVVS---EGFGFTKVELRPRL 181
Query: 185 AELTFNVVMRMVAGKSYFGE--NVSNEEEAKQFRYIMKEIFMISEASSPGDFIPIWNWID 244
E+TFN +MRM++GK Y+G+ +VS+ EEAKQFR I+ E+ + A++ GDF+P+ +D
Sbjct: 182 TEMTFNAMMRMISGKRYYGDDGDVSDVEEAKQFREIISEMMSLLGANNKGDFLPLLRVVD 241
Query: 245 PTGYEKKLKNIAKKVDIFLVNLIEEIR--NRKGEGNTMVDHLLSMQVSEPQFFSDQIIKG 304
EK+ K IAK+ + FL LIEE R N +G TM+DHLL + S+P+++SD +IKG
Sbjct: 242 LDNLEKRCKRIAKRSNAFLEGLIEEHRRGNIHSDGGTMIDHLLKLSESQPEYYSDHLIKG 301
Query: 305 LIWVIMLAGTDTQSGTLTWTMSHLLNNPEVLKKARAEIDTVVGRERLVEENDLSKMTYLQ 364
LI ++LAGTDT + T+ W MS LLN+PEVLKKA+ E+DT +G+ +LV+E DLSK+ YLQ
Sbjct: 302 LIQGMLLAGTDTSAVTIEWVMSELLNHPEVLKKAKEELDTQIGKNKLVDEQDLSKLPYLQ 361
Query: 365 QVIAETLRLN-PLPLLLPHCASEDCTIQGYHIPRDTMLLFNVWAIHRDPKLWDDALIFKP 424
+I+ETLRL+ P PLLLPH +SEDCTI +++P+DT++L NVW IHRDPK W+DAL FKP
Sbjct: 362 NIISETLRLHPPAPLLLPHYSSEDCTIGEFNVPKDTIILTNVWGIHRDPKHWNDALSFKP 421
Query: 425 ERHATLTIEDQYKYLPFGLGRRACPGGGMANRLVGLALATMIQCFEWKRVSDELVDMTVG 484
ER E+ K + FGLGRRACPG +A R VG + +IQCFEW+R S+E +DM G
Sbjct: 422 ERFE--KEEEVNKVMAFGLGRRACPGLSLAQRTVGFTVGLLIQCFEWERESEEKLDMMEG 481
Query: 485 NGITASRAFPLEAMCKPWP 496
GIT PL AMCK P
Sbjct: 482 KGITMPMKIPLRAMCKALP 494
BLAST of MC02g_new0213 vs. ExPASy Swiss-Prot
Match:
Q6WNR0 (Isoflavone 2'-hydroxylase OS=Medicago truncatula OX=3880 GN=CYP81E7 PE=1 SV=1)
HSP 1 Score: 496.9 bits (1278), Expect = 2.7e-139
Identity = 247/499 (49.50%), Postives = 348/499 (69.74%), Query Frame = 0
Query: 6 LYFSLSLLSI-LVVRFLLRSPSGRKLPPSPAFRLPIIGHLHLLGRPAQHAFKKLADKYGP 65
L +SL LSI ++R L +S + LPP P LPIIG+LH L RP FK L +KYG
Sbjct: 7 LCYSLFYLSIFFIIRLLFQSRKFKNLPPGPT-SLPIIGNLHHLKRPLNRTFKALTEKYGN 66
Query: 66 VFSLRFGSRLVVIITSPEAVKECFTKNDVVLAARPLLNSGKYMAYDNTIFAAASYGEHWR 125
V SL FGSRLVV+++S +ECFTKNDVVLA RP SGKY+ Y+ T + SYGEHWR
Sbjct: 67 VISLWFGSRLVVVVSSLSEFQECFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWR 126
Query: 126 NLRRIGSIEIYSPTRLNKFLDSRGDEVKRLLRRLGESNNSPPPASAGFSTVKLEPLFAEL 185
NLRRI S+++ S R+N F R DE +RL+++L E +S F+ V+L F ++
Sbjct: 127 NLRRITSLDVLSNHRINNFAPIRRDETQRLIKKLAED------SSTKFAEVELTFRFFDM 186
Query: 186 TFNVVMRMVAGKSYFGE--NVSNEEEAKQFRYIMKEIFMISEASSPGDFIPIWNWIDPTG 245
TFN +MRM++GK Y+G+ ++S +EA QFR ++ E+ +S A++ DF+P+ ++D
Sbjct: 187 TFNNIMRMISGKRYYGDDCDISEVQEASQFRDMVSELLQLSGANNKTDFMPLLKFLDFEN 246
Query: 246 YEKKLKNIAKKVDIFLVNLIEEIRNRKGEGNTMVDHLLSMQVSEPQFFSDQIIKGLIWVI 305
EK++K I +K D+FL L++E R++K NTM+DHLL+MQ S+P++++D IIKGL +
Sbjct: 247 LEKRVKRIGEKNDVFLSGLLQEQRSKKERTNTMIDHLLNMQESQPEYYTDTIIKGLCLAM 306
Query: 306 MLAGTDTQSGTLTWTMSHLLNNPEVLKKARAEIDTVVGRERLVEENDLSKMTYLQQVIAE 365
+LAGTD+ + TL WTMS++LN PEVLKK R E+DT VG++RLV+E+DL K+TYL+ VI E
Sbjct: 307 LLAGTDSSAVTLEWTMSNILNYPEVLKKVRDEVDTHVGQDRLVDESDLPKLTYLRNVIYE 366
Query: 366 TLRL-NPLPLLLPHCASEDCTIQGYHIPRDTMLLFNVWAIHRDPKLWDDALIFKPERHAT 425
TLRL P PLLLPH +++C + GY +PRDT++L N WAIHRDP+ W +A FKPER
Sbjct: 367 TLRLYTPAPLLLPHSTADECIMGGYKVPRDTIVLINAWAIHRDPETWSEATTFKPERFDK 426
Query: 426 LTIEDQYKYLPFGLGRRACPGGGMANRLVGLALATMIQCFEWKRVSDELVDMTVGNGITA 485
+ K + FG+GRRACPG G+A R + + LA ++QCF+WKR++DE +DM+ +G T
Sbjct: 427 K--GELEKMIAFGMGRRACPGEGLALRAISMTLALLVQCFDWKRINDEKIDMSERDGFTM 486
Query: 486 SRAFPLEAMCKPWPDMERV 501
++ PL+AMCK P + +V
Sbjct: 487 TKLLPLKAMCKTRPVVNKV 496
BLAST of MC02g_new0213 vs. ExPASy Swiss-Prot
Match:
Q9LHA1 (Cytochrome P450 81D11 OS=Arabidopsis thaliana OX=3702 GN=CYP81D11 PE=2 SV=1)
HSP 1 Score: 487.6 bits (1254), Expect = 1.7e-136
Identity = 251/507 (49.51%), Postives = 344/507 (67.85%), Query Frame = 0
Query: 1 MENILLYFSLSLLSILVVRFLLRSPSGRK--LPPSPAFRLPIIGHLHLLGRPAQHAFKKL 60
ME I L SL I + LL P RK LPPSP+ PIIGHLHLL P F L
Sbjct: 8 METIYLILSLFFFFIFLSLKLLFGPRPRKLNLPPSPSRPFPIIGHLHLLKLPLHRRFLSL 67
Query: 61 ADKY--GPVFSLRFGSRLVVIITSPEAVKECFTKNDVVLAARPLLNSGKYMAYDNTIFAA 120
++ +FSL GSRLV +++S +ECFTKNDVVLA RP GK++ Y++T
Sbjct: 68 SESLNNAKIFSLSLGSRLVFVVSSHAVAEECFTKNDVVLANRPEFLVGKHIGYNSTTMVG 127
Query: 121 ASYGEHWRNLRRIGSIEIYSPTRLNKFLDSRGDEVKRLLRRLGESNNSPPPASAGFSTVK 180
A+YG+ WRNLRRIG+IEI+S RLN F+ R DE++RL+ L +++ GF V+
Sbjct: 128 AAYGDSWRNLRRIGTIEIFSSLRLNSFVSIRQDEIRRLIICLAKNSQH------GFVKVE 187
Query: 181 LEPLFAELTFNVVMRMVAGKSYFGENVSNEEEAKQFRYIMKEIFMISEASSPGDFIPIWN 240
++PLF LT N ++RMVAGK ++G+ N+ EAK R ++ E+ + A + D+ PI
Sbjct: 188 MKPLFMCLTINNIIRMVAGKRFYGDGTENDNEAKHVRQLIAEVVVSGGAGNAADYFPILR 247
Query: 241 WIDPTGYEKKLKNIAKKVDIFLVNLIEEIRNRKGEGNTMVDHLLSMQVSEPQFFSDQIIK 300
++ T YEK +K +A +VD FL +L+ E R K +GNTM+DHLLS+Q ++P +++D IIK
Sbjct: 248 YV--TNYEKHVKKLAGRVDEFLQSLVNEKRVEKVKGNTMIDHLLSLQETQPDYYTDVIIK 307
Query: 301 GLIWVIMLAGTDTQSGTLTWTMSHLLNNPEVLKKARAEIDTVVGRERLVEENDLSKMTYL 360
G+I V++LAGTDT +GTL W MS+LLN+PEVL+KA+ EID +G +RLVEE D+ K+ YL
Sbjct: 308 GIILVMILAGTDTSAGTLEWAMSNLLNHPEVLRKAKTEIDDQIGVDRLVEEQDIVKLPYL 367
Query: 361 QQVIAETLRLNPL-PLLLPHCASEDCTIQGYHIPRDTMLLFNVWAIHRDPKLWDDALIFK 420
Q +++ETLRL P+ P+LLPH ASEDC + GY +PR T++L N WAIHRDPKLW++ FK
Sbjct: 368 QHIVSETLRLYPVAPMLLPHLASEDCIVDGYDVPRGTIILVNAWAIHRDPKLWEEPEKFK 427
Query: 421 PERHATLTIEDQYKYLPFGLGRRACPGGGMANRLVGLALATMIQCFEWKRVSDELVDMTV 480
PER ED+ K +PFG+GRR+CPG G+A RLV LAL +++QCFEW+RV ++ +DM
Sbjct: 428 PERFEKKG-EDK-KLMPFGIGRRSCPGSGLAQRLVTLALGSLVQCFEWERVEEKYLDMRE 487
Query: 481 G-NGITASRAFPLEAMCKPWPDMERVL 502
G T +A L+AMCK P + +VL
Sbjct: 488 SEKGTTMRKATSLQAMCKARPIVHKVL 504
BLAST of MC02g_new0213 vs. NCBI nr
Match:
XP_022149523.1 (isoflavone 2'-hydroxylase-like [Momordica charantia] >BBI21928.1 cytochrome P450 monooxygenase [Momordica charantia])
HSP 1 Score: 1031 bits (2666), Expect = 0.0
Identity = 510/510 (100.00%), Postives = 510/510 (100.00%), Query Frame = 0
Query: 1 MENILLYFSLSLLSILVVRFLLRSPSGRKLPPSPAFRLPIIGHLHLLGRPAQHAFKKLAD 60
MENILLYFSLSLLSILVVRFLLRSPSGRKLPPSPAFRLPIIGHLHLLGRPAQHAFKKLAD
Sbjct: 1 MENILLYFSLSLLSILVVRFLLRSPSGRKLPPSPAFRLPIIGHLHLLGRPAQHAFKKLAD 60
Query: 61 KYGPVFSLRFGSRLVVIITSPEAVKECFTKNDVVLAARPLLNSGKYMAYDNTIFAAASYG 120
KYGPVFSLRFGSRLVVIITSPEAVKECFTKNDVVLAARPLLNSGKYMAYDNTIFAAASYG
Sbjct: 61 KYGPVFSLRFGSRLVVIITSPEAVKECFTKNDVVLAARPLLNSGKYMAYDNTIFAAASYG 120
Query: 121 EHWRNLRRIGSIEIYSPTRLNKFLDSRGDEVKRLLRRLGESNNSPPPASAGFSTVKLEPL 180
EHWRNLRRIGSIEIYSPTRLNKFLDSRGDEVKRLLRRLGESNNSPPPASAGFSTVKLEPL
Sbjct: 121 EHWRNLRRIGSIEIYSPTRLNKFLDSRGDEVKRLLRRLGESNNSPPPASAGFSTVKLEPL 180
Query: 181 FAELTFNVVMRMVAGKSYFGENVSNEEEAKQFRYIMKEIFMISEASSPGDFIPIWNWIDP 240
FAELTFNVVMRMVAGKSYFGENVSNEEEAKQFRYIMKEIFMISEASSPGDFIPIWNWIDP
Sbjct: 181 FAELTFNVVMRMVAGKSYFGENVSNEEEAKQFRYIMKEIFMISEASSPGDFIPIWNWIDP 240
Query: 241 TGYEKKLKNIAKKVDIFLVNLIEEIRNRKGEGNTMVDHLLSMQVSEPQFFSDQIIKGLIW 300
TGYEKKLKNIAKKVDIFLVNLIEEIRNRKGEGNTMVDHLLSMQVSEPQFFSDQIIKGLIW
Sbjct: 241 TGYEKKLKNIAKKVDIFLVNLIEEIRNRKGEGNTMVDHLLSMQVSEPQFFSDQIIKGLIW 300
Query: 301 VIMLAGTDTQSGTLTWTMSHLLNNPEVLKKARAEIDTVVGRERLVEENDLSKMTYLQQVI 360
VIMLAGTDTQSGTLTWTMSHLLNNPEVLKKARAEIDTVVGRERLVEENDLSKMTYLQQVI
Sbjct: 301 VIMLAGTDTQSGTLTWTMSHLLNNPEVLKKARAEIDTVVGRERLVEENDLSKMTYLQQVI 360
Query: 361 AETLRLNPLPLLLPHCASEDCTIQGYHIPRDTMLLFNVWAIHRDPKLWDDALIFKPERHA 420
AETLRLNPLPLLLPHCASEDCTIQGYHIPRDTMLLFNVWAIHRDPKLWDDALIFKPERHA
Sbjct: 361 AETLRLNPLPLLLPHCASEDCTIQGYHIPRDTMLLFNVWAIHRDPKLWDDALIFKPERHA 420
Query: 421 TLTIEDQYKYLPFGLGRRACPGGGMANRLVGLALATMIQCFEWKRVSDELVDMTVGNGIT 480
TLTIEDQYKYLPFGLGRRACPGGGMANRLVGLALATMIQCFEWKRVSDELVDMTVGNGIT
Sbjct: 421 TLTIEDQYKYLPFGLGRRACPGGGMANRLVGLALATMIQCFEWKRVSDELVDMTVGNGIT 480
Query: 481 ASRAFPLEAMCKPWPDMERVLVEEVGLVRK 510
ASRAFPLEAMCKPWPDMERVLVEEVGLVRK
Sbjct: 481 ASRAFPLEAMCKPWPDMERVLVEEVGLVRK 510
BLAST of MC02g_new0213 vs. NCBI nr
Match:
XP_022149460.1 (isoflavone 2'-hydroxylase-like [Momordica charantia])
HSP 1 Score: 605 bits (1561), Expect = 1.76e-210
Identity = 295/504 (58.53%), Postives = 378/504 (75.00%), Query Frame = 0
Query: 1 MENILLYFSLSLLSILVVRFLLRSPSGRKLPPSPAFRLPIIGHLHLLGRPAQHAFKKLAD 60
M+ +LL SLSL S+ + L P R LPPSP FRLP+IGHLHL+ P KL+
Sbjct: 81 MDILLLSISLSLFSLFIFFHFLLRPYRRNLPPSPFFRLPLIGHLHLVKHPVHRTLHKLSH 140
Query: 61 KYGPVFSLRFGSRLVVIITSPEAVKECFTKNDVVLAARPLLNSGKYMAYDNTIFAAASYG 120
YG VFSLR+GSRLVV+++SP AV+ECFTKND+VLA RPLL SGK++AY++T A + YG
Sbjct: 141 NYGHVFSLRYGSRLVVVVSSPSAVQECFTKNDIVLANRPLLKSGKFLAYNHTTLAVSPYG 200
Query: 121 EHWRNLRRIGSIEIYSPTRLNKFLDSRGDEVKRLLRRL-GESNNSPPPASAGFSTVKLEP 180
EHWRNLRRI ++EI+S +RLN FL R DEVK LLR+L G+S F V+L+
Sbjct: 201 EHWRNLRRISTLEIFSTSRLNLFLGIREDEVKNLLRKLRGDSLQE-------FRAVELDS 260
Query: 181 LFAELTFNVVMRMVAGKSYFGENVSNEEEAKQFRYIMKEIFMISEASSPGDFIPIWNWID 240
+ + TFN+V+RM+AGK Y+GE++ +E ++K+FR ++KEI ++ GDF+P+WNWID
Sbjct: 261 MLLDHTFNIVVRMIAGKKYYGEDLRDEGQSKKFREMVKEILANGGTANLGDFVPLWNWID 320
Query: 241 PTGYEKKLKNIAKKVDIFLVNLIEEIRNRKGEGNTMVDHLLSMQVSEPQFFSDQIIKGLI 300
PTGYEK++ I ++ D L L++EIRN+K GNTM+DHLLS+Q ++P++FSDQIIKGL
Sbjct: 321 PTGYEKRIMKIGERSDELLQELVDEIRNQKDGGNTMIDHLLSLQNTQPEYFSDQIIKGLA 380
Query: 301 WVIMLAGTDTQSGTLTWTMSHLLNNPEVLKKARAEIDTVVGRERLVEENDLSKMTYLQQV 360
+++LAG DT + TL W +S LLNNPEVLKKARAEID +G +RLV E DLS ++YLQ V
Sbjct: 381 QILLLAGIDTAAITLEWALSQLLNNPEVLKKARAEIDHFIGEDRLVSEGDLSSLSYLQGV 440
Query: 361 IAETLRLNPL-PLLLPHCASEDCTIQGYHIPRDTMLLFNVWAIHRDPKLWDDALIFKPER 420
I ETLRLNP PLL+PHCASEDC I+GY IPRDT++L N WAIHRD +W+DA FKPER
Sbjct: 441 ITETLRLNPAAPLLVPHCASEDCKIEGYDIPRDTIILINAWAIHRDSNIWEDATSFKPER 500
Query: 421 HA-TLTIEDQYKYLPFGLGRRACPGGGMANRLVGLALATMIQCFEWKRVSDELVDMTVGN 480
HA T+ D YK LPFGLGRRACPG GMA R+VGL LA++IQCFEW+RV+ LVDMT G
Sbjct: 501 HANTMGGVDSYKLLPFGLGRRACPGMGMAQRVVGLTLASLIQCFEWERVNSSLVDMTEGE 560
Query: 481 GITASRAFPLEAMCKPWPDMERVL 501
G+T +A PL A CKP P ++ +L
Sbjct: 561 GLTMPKAQPLVAKCKPRPILKAIL 577
BLAST of MC02g_new0213 vs. NCBI nr
Match:
XP_022149455.1 (cytochrome P450 81E8-like [Momordica charantia])
HSP 1 Score: 590 bits (1521), Expect = 9.13e-206
Identity = 294/507 (57.99%), Postives = 377/507 (74.36%), Query Frame = 0
Query: 1 MENILLYFSLSLLSILVV-RFLLRSPSGRKLPPSPAFRLPIIGHLHLLGRPAQHAFKKLA 60
M+ + L SLSL S+L+ FL RS + LPPSP F LPI+GHLHL+ P F +L+
Sbjct: 1 MDILFLSISLSLFSLLLAFSFLFRS-RRQNLPPSPLFSLPIVGHLHLVKHPVHQTFHELS 60
Query: 61 DKYGPVFSLRFGSRLVVIITSPEAVKECFTKNDVVLAARPLLNSGKYMAYDNTIFAAASY 120
KYG VFSLRFGSR+VV+++SP AV+ECFTKND+VLA RPLL SGK +AY++T A A Y
Sbjct: 61 HKYGHVFSLRFGSRVVVVVSSPSAVQECFTKNDIVLANRPLLKSGKLLAYNHTTMAVAPY 120
Query: 121 GEHWRNLRRIGSIEIYSPTRLNKFLDSRGDEVKRLLRRLGESNNSPPPASAGFSTVKLEP 180
GEHWRNLRRIG++EI+S TRLN FL R DEV+ L+ +L E++ F+ V+LEP
Sbjct: 121 GEHWRNLRRIGTLEIFSTTRLNMFLGIREDEVRNLMLKLCENSFEE------FTAVELEP 180
Query: 181 LFAELTFNVVMRMVAGKSYFGENVSNEEEAKQFRYIMKEIFMISEASSPGDFIPIWNWID 240
+ +LTFN+VMRMVAGK Y+ E+++K+FR ++ ++ A++PGDFIPIWNWID
Sbjct: 181 MLFDLTFNIVMRMVAGKKYY-----EEQQSKKFREMVTQVLAHVGATNPGDFIPIWNWID 240
Query: 241 PTGYEKKLKNIAKKVDIFLVNLIEEIRNRKGEGNTMVDHLLSMQVSEPQFFSDQIIKGLI 300
PTG+EK++ + ++ D L LI+EIRN++G NTM+DHLL +Q +EP+++SDQIIKGLI
Sbjct: 241 PTGFEKRIIKLGQRSDELLQELIDEIRNQEGGDNTMIDHLLCLQKNEPEYYSDQIIKGLI 300
Query: 301 WVIMLAGTDTQSGTLTWTMSHLLNNPEVLKKARAEIDTVVGRERLVEENDLSKMTYLQQV 360
VI+LAG DT + TL W +S LLNNPEVL+KA+AEID VG+ERLV E DLS + YLQ +
Sbjct: 301 QVILLAGIDTAAVTLEWALSRLLNNPEVLEKAKAEIDCFVGQERLVNEADLSSLIYLQGI 360
Query: 361 IAETLRLNPL-PLLLPHCASEDCTIQGYHIPRDTMLLFNVWAIHRDPKLWDDALIFKPER 420
I ETLRLNP PLL+PHCAS DC I GY +PRDT++L N+WAIHRDP LW+DA FKPER
Sbjct: 361 IWETLRLNPAAPLLIPHCASADCKIGGYDVPRDTIILINIWAIHRDPNLWEDATNFKPER 420
Query: 421 HATLTIEDQYKYLPFGLGRRACPGGGMANRLVGLALATMIQCFEWKRVSDELVDMTVGNG 480
H D YK LPFG GRR+CPG GMA R+VGL LA++IQ FEW+R+S+ LVDMT G G
Sbjct: 421 HGNTIGVDSYKLLPFGSGRRSCPGVGMAQRVVGLTLASLIQGFEWERMSNSLVDMTEGKG 480
Query: 481 ITASRAFPLEAMCKPWPDMERVLVEEV 505
+T +A L A CKP P M+ +L E +
Sbjct: 481 LTMPKAQHLVAKCKPRPIMKLILNEAI 495
BLAST of MC02g_new0213 vs. NCBI nr
Match:
XP_030926572.1 (cytochrome P450 81Q32-like isoform X1 [Quercus lobata])
HSP 1 Score: 588 bits (1516), Expect = 6.71e-205
Identity = 290/505 (57.43%), Postives = 384/505 (76.04%), Query Frame = 0
Query: 1 MENILLYFSLSLLSILVVRF--LLRSPSGRK-LPPSPAFRLPIIGHLHLLGRPAQHAFKK 60
ME+ILLY SLS L +L + F L++ + RK LPPSP LPI+GHLHLL +P F +
Sbjct: 1 MEDILLYSSLSSLLLLFIAFKIFLQTRTQRKHLPPSPP-SLPILGHLHLLKKPLHRTFHR 60
Query: 61 LADKYGPVFSLRFGSRLVVIITSPEAVKECFTKNDVVLAARPLLNSGKYMAYDNTIFAAA 120
L+ KYG VFSLRFGS+LVVII+SP AV+ECFTKND+VLA RP + G+++ Y+ T +A
Sbjct: 61 LSQKYGHVFSLRFGSKLVVIISSPSAVEECFTKNDIVLANRPRFSVGRHLGYNYTTMVSA 120
Query: 121 SYGEHWRNLRRIGSIEIYSPTRLNKFLDSRGDEVKRLLRRLGESNNSPPPASAGFSTVKL 180
YG+HWRNLRRI ++EI+S RLN FL R DE+K LL +L SNNS + GF+ V+L
Sbjct: 121 PYGDHWRNLRRISALEIFSTNRLNMFLGIRRDEIKHLLHKL--SNNS----TQGFAKVEL 180
Query: 181 EPLFAELTFNVVMRMVAGKSYFGENVSNEEEAKQFRYIMKEIFMISEASSPGDFIPIWNW 240
+ +F+ELTFN++MRMV GK Y+GE+V + EEA+QFR IM EI ++ AS+PG+F+ + W
Sbjct: 181 KSMFSELTFNIIMRMVGGKRYYGEDVKDVEEARQFREIMNEIVKLAGASNPGEFVAVLRW 240
Query: 241 IDPTGYEKKLKNIAKKVDIFLVNLIEEIRNRKGEGNTMVDHLLSMQVSEPQFFSDQIIKG 300
ID G EKKLK +AK+ D+FL +LI+E+RN++ EGNTM+DHLLS+Q S+P++++DQIIKG
Sbjct: 241 IDHGGLEKKLKGLAKRTDVFLQSLIDEVRNKEEEGNTMIDHLLSLQKSQPEYYTDQIIKG 300
Query: 301 LIWVIMLAGTDTQSGTLTWTMSHLLNNPEVLKKARAEIDTVVGRERLVEENDLSKMTYLQ 360
L+ V++LAGTDT + L W MS+L+N+P+VLKKAR ++D +G+E+L++E D+SK+ YLQ
Sbjct: 301 LMMVLILAGTDTSAVILEWVMSNLINHPDVLKKARVDLDNQIGQEKLIDELDVSKLHYLQ 360
Query: 361 QVIAETLRLNPL-PLLLPHCASEDCTIQGYHIPRDTMLLFNVWAIHRDPKLWDDALIFKP 420
+I+ET RL P PLL+PH +S DCTI GY IPRDT+LL N WAIHRDP+LW+D FKP
Sbjct: 361 CIISETFRLYPAAPLLVPHFSSNDCTIGGYDIPRDTILLVNAWAIHRDPELWEDTTSFKP 420
Query: 421 ERHATLTIEDQYKYLPFGLGRRACPGGGMANRLVGLALATMIQCFEWKRVSDELVDMTVG 480
ER D YK +PFGLGRRACPG G+A R + L L ++IQCFEW+RV++E VDMT G
Sbjct: 421 ERFEIGEC-DPYKLMPFGLGRRACPGAGLAQRTISLTLGSLIQCFEWERVTNEKVDMTEG 480
Query: 481 NGITASRAFPLEAMCKPWPDMERVL 501
NGIT + PLEAMCK P M ++L
Sbjct: 481 NGITMPKVIPLEAMCKARPIMHKIL 497
BLAST of MC02g_new0213 vs. NCBI nr
Match:
XP_038901746.1 (cytochrome P450 81Q32-like [Benincasa hispida])
HSP 1 Score: 588 bits (1515), Expect = 1.02e-204
Identity = 292/507 (57.59%), Postives = 372/507 (73.37%), Query Frame = 0
Query: 1 MENILLYFSLSLLSILVVRFLLRSPSGRKLPPSPAFRLPIIGHLHLLGRPAQHAFKKLAD 60
M+NIL+Y L ++S+L+ LL P G+ LPPSP F LP+IGHLHLL P ++
Sbjct: 1 MDNILVYIFLFVISLLLALKLLL-PHGKNLPPSPLFSLPLIGHLHLLKHPIHQNLHNHSN 60
Query: 61 KYGPVFSLRFGSRLVVIITSPEAVKECFTKNDVVLAARPLLNSGKYMAYDNTIFAAASYG 120
KYG +FSL FGSRLVV+++SP AV+ECFTKND+VLA RP LN+GK++ Y+ T+ A A YG
Sbjct: 61 KYGRIFSLWFGSRLVVVVSSPSAVRECFTKNDIVLANRPFLNTGKHLTYNFTVLAVAPYG 120
Query: 121 EHWRNLRRIGSIEIYSPTRLNKFLDSRGDEVKRLLRRLGESNNSPPPASAGFSTVKLEPL 180
E WR+LRRI + EI+S +RLN F R DEVKRLL +L N+ FS V++EP+
Sbjct: 121 ELWRSLRRISTCEIFSTSRLNLFSGIRQDEVKRLLCKLCSGNSVEELLL--FSVVEVEPM 180
Query: 181 FAELTFNVVMRMVAGKSYFGENVSNEEEAKQFRYIMKEIFMISEASSPGDFIPIWNWIDP 240
+ T N+VMRMVAGK YFG++V NE +A +FR ++K + M + A++PGDFIP+WNWIDP
Sbjct: 181 LLDFTSNIVMRMVAGKRYFGDDVLNEGQAGKFRDVVKRVMMYAGATNPGDFIPLWNWIDP 240
Query: 241 TGYEKKLKNIAKKVDIFLVNLIEEIRNRKGEGNTMVDHLLSMQVSEPQFFSDQIIKGLIW 300
TG EKK+ + ++ D LI+EIR ++G GNTM+ HLL +Q + P++FSDQIIKGLI
Sbjct: 241 TGLEKKIIKVGQEADAIFQGLIDEIRTQEG-GNTMIHHLLHLQTTRPEYFSDQIIKGLIH 300
Query: 301 VIMLAGTDTQSGTLTWTMSHLLNNPEVLKKARAEIDTVVGRERLVEENDLSKMTYLQQVI 360
VI+LAG DT + TL W +SHL NNPEV+KKAR EI+ G+ERLV E DLS ++YLQ ++
Sbjct: 301 VILLAGIDTTAVTLEWALSHLFNNPEVMKKARLEIECCTGQERLVNEEDLSSLSYLQGIV 360
Query: 361 AETLRLNPL-PLLLPHCASEDCTIQGYHIPRDTMLLFNVWAIHRDPKLWDDALIFKPERH 420
ETLRL P PLL+PHCASEDC I+GY IPRDT++ N WAIHRDP LW+DA FKPERH
Sbjct: 361 LETLRLTPAAPLLVPHCASEDCQIEGYDIPRDTIIFVNAWAIHRDPNLWEDATSFKPERH 420
Query: 421 AT-LTIEDQYKYLPFGLGRRACPGGGMANRLVGLALATMIQCFEWKRVSDELVDMTVGNG 480
+ + D YK LPFGLGRRACPG GMA R+VGL LA++IQCF+W+ + LVDMT G+G
Sbjct: 421 ENAIELFDSYKLLPFGLGRRACPGVGMAQRVVGLTLASLIQCFDWETMDGSLVDMTEGHG 480
Query: 481 ITASRAFPLEAMCKPWPDMERVLVEEV 505
IT +A PL A CKP P M R LV EV
Sbjct: 481 ITMPKAQPLVAKCKPRPIM-RALVNEV 502
BLAST of MC02g_new0213 vs. ExPASy TrEMBL
Match:
A0A5K7U6A3 (Cytochrome P450 monooxygenase OS=Momordica charantia OX=3673 GN=McCYP81AQ19 PE=2 SV=1)
HSP 1 Score: 1031 bits (2666), Expect = 0.0
Identity = 510/510 (100.00%), Postives = 510/510 (100.00%), Query Frame = 0
Query: 1 MENILLYFSLSLLSILVVRFLLRSPSGRKLPPSPAFRLPIIGHLHLLGRPAQHAFKKLAD 60
MENILLYFSLSLLSILVVRFLLRSPSGRKLPPSPAFRLPIIGHLHLLGRPAQHAFKKLAD
Sbjct: 1 MENILLYFSLSLLSILVVRFLLRSPSGRKLPPSPAFRLPIIGHLHLLGRPAQHAFKKLAD 60
Query: 61 KYGPVFSLRFGSRLVVIITSPEAVKECFTKNDVVLAARPLLNSGKYMAYDNTIFAAASYG 120
KYGPVFSLRFGSRLVVIITSPEAVKECFTKNDVVLAARPLLNSGKYMAYDNTIFAAASYG
Sbjct: 61 KYGPVFSLRFGSRLVVIITSPEAVKECFTKNDVVLAARPLLNSGKYMAYDNTIFAAASYG 120
Query: 121 EHWRNLRRIGSIEIYSPTRLNKFLDSRGDEVKRLLRRLGESNNSPPPASAGFSTVKLEPL 180
EHWRNLRRIGSIEIYSPTRLNKFLDSRGDEVKRLLRRLGESNNSPPPASAGFSTVKLEPL
Sbjct: 121 EHWRNLRRIGSIEIYSPTRLNKFLDSRGDEVKRLLRRLGESNNSPPPASAGFSTVKLEPL 180
Query: 181 FAELTFNVVMRMVAGKSYFGENVSNEEEAKQFRYIMKEIFMISEASSPGDFIPIWNWIDP 240
FAELTFNVVMRMVAGKSYFGENVSNEEEAKQFRYIMKEIFMISEASSPGDFIPIWNWIDP
Sbjct: 181 FAELTFNVVMRMVAGKSYFGENVSNEEEAKQFRYIMKEIFMISEASSPGDFIPIWNWIDP 240
Query: 241 TGYEKKLKNIAKKVDIFLVNLIEEIRNRKGEGNTMVDHLLSMQVSEPQFFSDQIIKGLIW 300
TGYEKKLKNIAKKVDIFLVNLIEEIRNRKGEGNTMVDHLLSMQVSEPQFFSDQIIKGLIW
Sbjct: 241 TGYEKKLKNIAKKVDIFLVNLIEEIRNRKGEGNTMVDHLLSMQVSEPQFFSDQIIKGLIW 300
Query: 301 VIMLAGTDTQSGTLTWTMSHLLNNPEVLKKARAEIDTVVGRERLVEENDLSKMTYLQQVI 360
VIMLAGTDTQSGTLTWTMSHLLNNPEVLKKARAEIDTVVGRERLVEENDLSKMTYLQQVI
Sbjct: 301 VIMLAGTDTQSGTLTWTMSHLLNNPEVLKKARAEIDTVVGRERLVEENDLSKMTYLQQVI 360
Query: 361 AETLRLNPLPLLLPHCASEDCTIQGYHIPRDTMLLFNVWAIHRDPKLWDDALIFKPERHA 420
AETLRLNPLPLLLPHCASEDCTIQGYHIPRDTMLLFNVWAIHRDPKLWDDALIFKPERHA
Sbjct: 361 AETLRLNPLPLLLPHCASEDCTIQGYHIPRDTMLLFNVWAIHRDPKLWDDALIFKPERHA 420
Query: 421 TLTIEDQYKYLPFGLGRRACPGGGMANRLVGLALATMIQCFEWKRVSDELVDMTVGNGIT 480
TLTIEDQYKYLPFGLGRRACPGGGMANRLVGLALATMIQCFEWKRVSDELVDMTVGNGIT
Sbjct: 421 TLTIEDQYKYLPFGLGRRACPGGGMANRLVGLALATMIQCFEWKRVSDELVDMTVGNGIT 480
Query: 481 ASRAFPLEAMCKPWPDMERVLVEEVGLVRK 510
ASRAFPLEAMCKPWPDMERVLVEEVGLVRK
Sbjct: 481 ASRAFPLEAMCKPWPDMERVLVEEVGLVRK 510
BLAST of MC02g_new0213 vs. ExPASy TrEMBL
Match:
A0A6J1D5S6 (isoflavone 2'-hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111017884 PE=3 SV=1)
HSP 1 Score: 605 bits (1561), Expect = 8.51e-211
Identity = 295/504 (58.53%), Postives = 378/504 (75.00%), Query Frame = 0
Query: 1 MENILLYFSLSLLSILVVRFLLRSPSGRKLPPSPAFRLPIIGHLHLLGRPAQHAFKKLAD 60
M+ +LL SLSL S+ + L P R LPPSP FRLP+IGHLHL+ P KL+
Sbjct: 81 MDILLLSISLSLFSLFIFFHFLLRPYRRNLPPSPFFRLPLIGHLHLVKHPVHRTLHKLSH 140
Query: 61 KYGPVFSLRFGSRLVVIITSPEAVKECFTKNDVVLAARPLLNSGKYMAYDNTIFAAASYG 120
YG VFSLR+GSRLVV+++SP AV+ECFTKND+VLA RPLL SGK++AY++T A + YG
Sbjct: 141 NYGHVFSLRYGSRLVVVVSSPSAVQECFTKNDIVLANRPLLKSGKFLAYNHTTLAVSPYG 200
Query: 121 EHWRNLRRIGSIEIYSPTRLNKFLDSRGDEVKRLLRRL-GESNNSPPPASAGFSTVKLEP 180
EHWRNLRRI ++EI+S +RLN FL R DEVK LLR+L G+S F V+L+
Sbjct: 201 EHWRNLRRISTLEIFSTSRLNLFLGIREDEVKNLLRKLRGDSLQE-------FRAVELDS 260
Query: 181 LFAELTFNVVMRMVAGKSYFGENVSNEEEAKQFRYIMKEIFMISEASSPGDFIPIWNWID 240
+ + TFN+V+RM+AGK Y+GE++ +E ++K+FR ++KEI ++ GDF+P+WNWID
Sbjct: 261 MLLDHTFNIVVRMIAGKKYYGEDLRDEGQSKKFREMVKEILANGGTANLGDFVPLWNWID 320
Query: 241 PTGYEKKLKNIAKKVDIFLVNLIEEIRNRKGEGNTMVDHLLSMQVSEPQFFSDQIIKGLI 300
PTGYEK++ I ++ D L L++EIRN+K GNTM+DHLLS+Q ++P++FSDQIIKGL
Sbjct: 321 PTGYEKRIMKIGERSDELLQELVDEIRNQKDGGNTMIDHLLSLQNTQPEYFSDQIIKGLA 380
Query: 301 WVIMLAGTDTQSGTLTWTMSHLLNNPEVLKKARAEIDTVVGRERLVEENDLSKMTYLQQV 360
+++LAG DT + TL W +S LLNNPEVLKKARAEID +G +RLV E DLS ++YLQ V
Sbjct: 381 QILLLAGIDTAAITLEWALSQLLNNPEVLKKARAEIDHFIGEDRLVSEGDLSSLSYLQGV 440
Query: 361 IAETLRLNPL-PLLLPHCASEDCTIQGYHIPRDTMLLFNVWAIHRDPKLWDDALIFKPER 420
I ETLRLNP PLL+PHCASEDC I+GY IPRDT++L N WAIHRD +W+DA FKPER
Sbjct: 441 ITETLRLNPAAPLLVPHCASEDCKIEGYDIPRDTIILINAWAIHRDSNIWEDATSFKPER 500
Query: 421 HA-TLTIEDQYKYLPFGLGRRACPGGGMANRLVGLALATMIQCFEWKRVSDELVDMTVGN 480
HA T+ D YK LPFGLGRRACPG GMA R+VGL LA++IQCFEW+RV+ LVDMT G
Sbjct: 501 HANTMGGVDSYKLLPFGLGRRACPGMGMAQRVVGLTLASLIQCFEWERVNSSLVDMTEGE 560
Query: 481 GITASRAFPLEAMCKPWPDMERVL 501
G+T +A PL A CKP P ++ +L
Sbjct: 561 GLTMPKAQPLVAKCKPRPILKAIL 577
BLAST of MC02g_new0213 vs. ExPASy TrEMBL
Match:
A0A6J1D5S1 (cytochrome P450 81E8-like OS=Momordica charantia OX=3673 GN=LOC111017881 PE=3 SV=1)
HSP 1 Score: 590 bits (1521), Expect = 4.42e-206
Identity = 294/507 (57.99%), Postives = 377/507 (74.36%), Query Frame = 0
Query: 1 MENILLYFSLSLLSILVV-RFLLRSPSGRKLPPSPAFRLPIIGHLHLLGRPAQHAFKKLA 60
M+ + L SLSL S+L+ FL RS + LPPSP F LPI+GHLHL+ P F +L+
Sbjct: 1 MDILFLSISLSLFSLLLAFSFLFRS-RRQNLPPSPLFSLPIVGHLHLVKHPVHQTFHELS 60
Query: 61 DKYGPVFSLRFGSRLVVIITSPEAVKECFTKNDVVLAARPLLNSGKYMAYDNTIFAAASY 120
KYG VFSLRFGSR+VV+++SP AV+ECFTKND+VLA RPLL SGK +AY++T A A Y
Sbjct: 61 HKYGHVFSLRFGSRVVVVVSSPSAVQECFTKNDIVLANRPLLKSGKLLAYNHTTMAVAPY 120
Query: 121 GEHWRNLRRIGSIEIYSPTRLNKFLDSRGDEVKRLLRRLGESNNSPPPASAGFSTVKLEP 180
GEHWRNLRRIG++EI+S TRLN FL R DEV+ L+ +L E++ F+ V+LEP
Sbjct: 121 GEHWRNLRRIGTLEIFSTTRLNMFLGIREDEVRNLMLKLCENSFEE------FTAVELEP 180
Query: 181 LFAELTFNVVMRMVAGKSYFGENVSNEEEAKQFRYIMKEIFMISEASSPGDFIPIWNWID 240
+ +LTFN+VMRMVAGK Y+ E+++K+FR ++ ++ A++PGDFIPIWNWID
Sbjct: 181 MLFDLTFNIVMRMVAGKKYY-----EEQQSKKFREMVTQVLAHVGATNPGDFIPIWNWID 240
Query: 241 PTGYEKKLKNIAKKVDIFLVNLIEEIRNRKGEGNTMVDHLLSMQVSEPQFFSDQIIKGLI 300
PTG+EK++ + ++ D L LI+EIRN++G NTM+DHLL +Q +EP+++SDQIIKGLI
Sbjct: 241 PTGFEKRIIKLGQRSDELLQELIDEIRNQEGGDNTMIDHLLCLQKNEPEYYSDQIIKGLI 300
Query: 301 WVIMLAGTDTQSGTLTWTMSHLLNNPEVLKKARAEIDTVVGRERLVEENDLSKMTYLQQV 360
VI+LAG DT + TL W +S LLNNPEVL+KA+AEID VG+ERLV E DLS + YLQ +
Sbjct: 301 QVILLAGIDTAAVTLEWALSRLLNNPEVLEKAKAEIDCFVGQERLVNEADLSSLIYLQGI 360
Query: 361 IAETLRLNPL-PLLLPHCASEDCTIQGYHIPRDTMLLFNVWAIHRDPKLWDDALIFKPER 420
I ETLRLNP PLL+PHCAS DC I GY +PRDT++L N+WAIHRDP LW+DA FKPER
Sbjct: 361 IWETLRLNPAAPLLIPHCASADCKIGGYDVPRDTIILINIWAIHRDPNLWEDATNFKPER 420
Query: 421 HATLTIEDQYKYLPFGLGRRACPGGGMANRLVGLALATMIQCFEWKRVSDELVDMTVGNG 480
H D YK LPFG GRR+CPG GMA R+VGL LA++IQ FEW+R+S+ LVDMT G G
Sbjct: 421 HGNTIGVDSYKLLPFGSGRRSCPGVGMAQRVVGLTLASLIQGFEWERMSNSLVDMTEGKG 480
Query: 481 ITASRAFPLEAMCKPWPDMERVLVEEV 505
+T +A L A CKP P M+ +L E +
Sbjct: 481 LTMPKAQHLVAKCKPRPIMKLILNEAI 495
BLAST of MC02g_new0213 vs. ExPASy TrEMBL
Match:
A0A7N2R7H8 (Uncharacterized protein OS=Quercus lobata OX=97700 PE=3 SV=1)
HSP 1 Score: 588 bits (1516), Expect = 3.25e-205
Identity = 290/505 (57.43%), Postives = 384/505 (76.04%), Query Frame = 0
Query: 1 MENILLYFSLSLLSILVVRF--LLRSPSGRK-LPPSPAFRLPIIGHLHLLGRPAQHAFKK 60
ME+ILLY SLS L +L + F L++ + RK LPPSP LPI+GHLHLL +P F +
Sbjct: 1 MEDILLYSSLSSLLLLFIAFKIFLQTRTQRKHLPPSPP-SLPILGHLHLLKKPLHRTFHR 60
Query: 61 LADKYGPVFSLRFGSRLVVIITSPEAVKECFTKNDVVLAARPLLNSGKYMAYDNTIFAAA 120
L+ KYG VFSLRFGS+LVVII+SP AV+ECFTKND+VLA RP + G+++ Y+ T +A
Sbjct: 61 LSQKYGHVFSLRFGSKLVVIISSPSAVEECFTKNDIVLANRPRFSVGRHLGYNYTTMVSA 120
Query: 121 SYGEHWRNLRRIGSIEIYSPTRLNKFLDSRGDEVKRLLRRLGESNNSPPPASAGFSTVKL 180
YG+HWRNLRRI ++EI+S RLN FL R DE+K LL +L SNNS + GF+ V+L
Sbjct: 121 PYGDHWRNLRRISALEIFSTNRLNMFLGIRRDEIKHLLHKL--SNNS----TQGFAKVEL 180
Query: 181 EPLFAELTFNVVMRMVAGKSYFGENVSNEEEAKQFRYIMKEIFMISEASSPGDFIPIWNW 240
+ +F+ELTFN++MRMV GK Y+GE+V + EEA+QFR IM EI ++ AS+PG+F+ + W
Sbjct: 181 KSMFSELTFNIIMRMVGGKRYYGEDVKDVEEARQFREIMNEIVKLAGASNPGEFVAVLRW 240
Query: 241 IDPTGYEKKLKNIAKKVDIFLVNLIEEIRNRKGEGNTMVDHLLSMQVSEPQFFSDQIIKG 300
ID G EKKLK +AK+ D+FL +LI+E+RN++ EGNTM+DHLLS+Q S+P++++DQIIKG
Sbjct: 241 IDHGGLEKKLKGLAKRTDVFLQSLIDEVRNKEEEGNTMIDHLLSLQKSQPEYYTDQIIKG 300
Query: 301 LIWVIMLAGTDTQSGTLTWTMSHLLNNPEVLKKARAEIDTVVGRERLVEENDLSKMTYLQ 360
L+ V++LAGTDT + L W MS+L+N+P+VLKKAR ++D +G+E+L++E D+SK+ YLQ
Sbjct: 301 LMMVLILAGTDTSAVILEWVMSNLINHPDVLKKARVDLDNQIGQEKLIDELDVSKLHYLQ 360
Query: 361 QVIAETLRLNPL-PLLLPHCASEDCTIQGYHIPRDTMLLFNVWAIHRDPKLWDDALIFKP 420
+I+ET RL P PLL+PH +S DCTI GY IPRDT+LL N WAIHRDP+LW+D FKP
Sbjct: 361 CIISETFRLYPAAPLLVPHFSSNDCTIGGYDIPRDTILLVNAWAIHRDPELWEDTTSFKP 420
Query: 421 ERHATLTIEDQYKYLPFGLGRRACPGGGMANRLVGLALATMIQCFEWKRVSDELVDMTVG 480
ER D YK +PFGLGRRACPG G+A R + L L ++IQCFEW+RV++E VDMT G
Sbjct: 421 ERFEIGEC-DPYKLMPFGLGRRACPGAGLAQRTISLTLGSLIQCFEWERVTNEKVDMTEG 480
Query: 481 NGITASRAFPLEAMCKPWPDMERVL 501
NGIT + PLEAMCK P M ++L
Sbjct: 481 NGITMPKVIPLEAMCKARPIMHKIL 497
BLAST of MC02g_new0213 vs. ExPASy TrEMBL
Match:
A0A6J1FUI0 (cytochrome P450 81D1-like OS=Cucurbita moschata OX=3662 GN=LOC111448921 PE=3 SV=1)
HSP 1 Score: 587 bits (1513), Expect = 7.50e-205
Identity = 291/505 (57.62%), Postives = 383/505 (75.84%), Query Frame = 0
Query: 1 MENILLYFSLSLLSILVVRFLLRSPSGRKLPPSPAFRLPIIGHLHLLGRPAQHAFKKLAD 60
MEN++LY L L++ + L PS KLPPSP F LPIIGHL+LL RP +KL++
Sbjct: 1 MENLMLYLCLPLMAFISSLIFLLRPST-KLPPSPPFSLPIIGHLYLLKRPVHQTLQKLSN 60
Query: 61 KYGPVFSLRFGSRLVVIITSPEAVKECFTKNDVVLAARPLLNSGKYMAYDNTIFAAASYG 120
KYG VFSLR GS+LVV+++SP AVKECFT ND++LA RP N K++ Y+NTIF+A+SYG
Sbjct: 61 KYGHVFSLRLGSQLVVVVSSPSAVKECFTMNDIILANRPSSNIIKHLTYNNTIFSASSYG 120
Query: 121 EHWRNLRRIGSIEIYSPTRLNKFLDSRGDEVKRLLRRLGESNNSPPPASAGFSTVKLEPL 180
+HWR LRRI +IEI+S +RLN FL++R DE+KR+L +L + S F+ V++ +
Sbjct: 121 DHWRELRRISTIEIFSASRLNMFLETRKDEIKRVLGKLCRIS------SDSFARVEMSSM 180
Query: 181 FAELTFNVVMRMVAGKSYFGENVSNEEEAKQFRYIMKEIFMISEASSPGDFIPIWNWIDP 240
+EL+ N+VMRMV GK ++GE V + E+A++FR I+K++ + FIP+ NWID
Sbjct: 181 ISELSSNIVMRMVMGKRFYGEGVLDLEQARKFRDIVKQVMQKCGSFDSRSFIPLLNWIDI 240
Query: 241 TGYEKKLKNIAKKVDIFLVNLIEEIRNRKGEG-NTMVDHLLSMQVSEPQFFSDQIIKGLI 300
T YEKK+ + ++ D FL LI+E RN+K EG NTM+DHLLS+Q S+ F SDQ+IKGLI
Sbjct: 241 THYEKKIIKLGQRNDEFLQGLIDEHRNQKEEGRNTMIDHLLSLQQSQLDFMSDQVIKGLI 300
Query: 301 WVIMLAGTDTQSGTLTWTMSHLLNNPEVLKKARAEIDTVVGRERLVEENDLSKMTYLQQV 360
VI+LAGTDT + T+ W +SHLLNNP VLKKAR E+D+++G+ERLVEE+D+SK++YLQ V
Sbjct: 301 MVILLAGTDTIAVTIEWALSHLLNNPNVLKKARIELDSIIGQERLVEESDMSKLSYLQGV 360
Query: 361 IAETLRLNPL-PLLLPHCASEDCTIQGYHIPRDTMLLFNVWAIHRDPKLWDDALIFKPER 420
I+ETLRLNP PLL+PH ASE+C I GY IPRDT++L N WAIHRDP +W+DA F PER
Sbjct: 361 ISETLRLNPAAPLLVPHYASENCWIAGYKIPRDTIILINAWAIHRDPNIWEDANTFNPER 420
Query: 421 HATLTIEDQYKYLPFGLGRRACPGGGMANRLVGLALATMIQCFEWKRVSDELVDMTVGNG 480
+ ++D YK +PFGLGRRACPG GMA+R+VGL+LA++IQCFEWKRVS E+VDMT G G
Sbjct: 421 YKITMVDDLYKLIPFGLGRRACPGVGMAHRVVGLSLASLIQCFEWKRVSSEVVDMTEGVG 480
Query: 481 ITASRAFPLEAMCKPWPDMERVLVE 503
+T RA PLEAMCK P +ERVL++
Sbjct: 481 LTIPRAQPLEAMCKSRPIVERVLMQ 498
BLAST of MC02g_new0213 vs. TAIR 10
Match:
AT3G28740.1 (Cytochrome P450 superfamily protein )
HSP 1 Score: 487.6 bits (1254), Expect = 1.2e-137
Identity = 251/507 (49.51%), Postives = 344/507 (67.85%), Query Frame = 0
Query: 1 MENILLYFSLSLLSILVVRFLLRSPSGRK--LPPSPAFRLPIIGHLHLLGRPAQHAFKKL 60
ME I L SL I + LL P RK LPPSP+ PIIGHLHLL P F L
Sbjct: 8 METIYLILSLFFFFIFLSLKLLFGPRPRKLNLPPSPSRPFPIIGHLHLLKLPLHRRFLSL 67
Query: 61 ADKY--GPVFSLRFGSRLVVIITSPEAVKECFTKNDVVLAARPLLNSGKYMAYDNTIFAA 120
++ +FSL GSRLV +++S +ECFTKNDVVLA RP GK++ Y++T
Sbjct: 68 SESLNNAKIFSLSLGSRLVFVVSSHAVAEECFTKNDVVLANRPEFLVGKHIGYNSTTMVG 127
Query: 121 ASYGEHWRNLRRIGSIEIYSPTRLNKFLDSRGDEVKRLLRRLGESNNSPPPASAGFSTVK 180
A+YG+ WRNLRRIG+IEI+S RLN F+ R DE++RL+ L +++ GF V+
Sbjct: 128 AAYGDSWRNLRRIGTIEIFSSLRLNSFVSIRQDEIRRLIICLAKNSQH------GFVKVE 187
Query: 181 LEPLFAELTFNVVMRMVAGKSYFGENVSNEEEAKQFRYIMKEIFMISEASSPGDFIPIWN 240
++PLF LT N ++RMVAGK ++G+ N+ EAK R ++ E+ + A + D+ PI
Sbjct: 188 MKPLFMCLTINNIIRMVAGKRFYGDGTENDNEAKHVRQLIAEVVVSGGAGNAADYFPILR 247
Query: 241 WIDPTGYEKKLKNIAKKVDIFLVNLIEEIRNRKGEGNTMVDHLLSMQVSEPQFFSDQIIK 300
++ T YEK +K +A +VD FL +L+ E R K +GNTM+DHLLS+Q ++P +++D IIK
Sbjct: 248 YV--TNYEKHVKKLAGRVDEFLQSLVNEKRVEKVKGNTMIDHLLSLQETQPDYYTDVIIK 307
Query: 301 GLIWVIMLAGTDTQSGTLTWTMSHLLNNPEVLKKARAEIDTVVGRERLVEENDLSKMTYL 360
G+I V++LAGTDT +GTL W MS+LLN+PEVL+KA+ EID +G +RLVEE D+ K+ YL
Sbjct: 308 GIILVMILAGTDTSAGTLEWAMSNLLNHPEVLRKAKTEIDDQIGVDRLVEEQDIVKLPYL 367
Query: 361 QQVIAETLRLNPL-PLLLPHCASEDCTIQGYHIPRDTMLLFNVWAIHRDPKLWDDALIFK 420
Q +++ETLRL P+ P+LLPH ASEDC + GY +PR T++L N WAIHRDPKLW++ FK
Sbjct: 368 QHIVSETLRLYPVAPMLLPHLASEDCIVDGYDVPRGTIILVNAWAIHRDPKLWEEPEKFK 427
Query: 421 PERHATLTIEDQYKYLPFGLGRRACPGGGMANRLVGLALATMIQCFEWKRVSDELVDMTV 480
PER ED+ K +PFG+GRR+CPG G+A RLV LAL +++QCFEW+RV ++ +DM
Sbjct: 428 PERFEKKG-EDK-KLMPFGIGRRSCPGSGLAQRLVTLALGSLVQCFEWERVEEKYLDMRE 487
Query: 481 G-NGITASRAFPLEAMCKPWPDMERVL 502
G T +A L+AMCK P + +VL
Sbjct: 488 SEKGTTMRKATSLQAMCKARPIVHKVL 504
BLAST of MC02g_new0213 vs. TAIR 10
Match:
AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8 )
HSP 1 Score: 487.6 bits (1254), Expect = 1.2e-137
Identity = 242/508 (47.64%), Postives = 347/508 (68.31%), Query Frame = 0
Query: 1 MENILLYFSLSLLSILVVRFLLRSPSGRKLPPSPAFRLPIIGHLHLLGRPAQHAFKKLAD 60
ME L FS+ + + ++ + + LPPSPA+ LP+IGHL LL P F L+
Sbjct: 1 METKTLIFSILFVVLSLIYLIGKLKRKPNLPPSPAWSLPVIGHLRLLKPPIHRTFLSLSQ 60
Query: 61 KY--GPVFSLRFGSRLVVIITSPEAVKECFTKNDVVLAARPLLNSGKYMAYDNTIFAAAS 120
P+FSLR G+RLV + +S +ECFTKNDVVLA RP K++AYD T AAS
Sbjct: 61 SLNNAPIFSLRLGNRLVFVNSSHSIAEECFTKNDVVLANRPNFILAKHVAYDYTTMIAAS 120
Query: 121 YGEHWRNLRRIGSIEIYSPTRLNKFLDSRGDEVKRLLRRLGESNNSPPPASAGFSTVKLE 180
YG+HWRNLRRIGS+EI+S RLN FL R DE++RL+ RL + S F V ++
Sbjct: 121 YGDHWRNLRRIGSVEIFSNHRLNSFLSIRKDEIRRLVFRLSRN------FSQEFVKVDMK 180
Query: 181 PLFAELTFNVVMRMVAGKSYFGENVSNEEEAKQFRYIMKEIFMISEASSPGDFIPIWNWI 240
+ ++LTFN ++RMVAGK Y+G+ V ++ EAK+ R ++ ++ + A + D++P+ +
Sbjct: 181 SMLSDLTFNNILRMVAGKRYYGDGVEDDPEAKRVRQLIADVVACAGAGNAVDYLPVLRLV 240
Query: 241 DPTGYEKKLKNIAKKVDIFLVNLIEEIRNRKGEGNTMVDHLLSMQVSEPQFFSDQIIKGL 300
+ YE ++K +A ++D FL L++E R K +GNTM+DHLL++Q S+P +F+D+IIKG
Sbjct: 241 --SDYETRVKKLAGRLDEFLQGLVDEKREAKEKGNTMIDHLLTLQESQPDYFTDRIIKGN 300
Query: 301 IWVIMLAGTDTQSGTLTWTMSHLLNNPEVLKKARAEIDTVVGRERLVEENDLSKMTYLQQ 360
+ ++LAGTDT + TL W +S++LN+P+VL KAR EID +G +RL++E+D+S + YLQ
Sbjct: 301 MLALILAGTDTSAVTLEWALSNVLNHPDVLNKARDEIDRKIGLDRLMDESDISNLPYLQN 360
Query: 361 VIAETLRLNP-LPLLLPHCASEDCTIQGYHIPRDTMLLFNVWAIHRDPKLWDDALIFKPE 420
+++ETLRL P P+LLPH ASEDC + GY +PR T+LL NVWAIHRDP+LWDD + FKPE
Sbjct: 361 IVSETLRLYPAAPMLLPHVASEDCKVAGYDMPRGTILLTNVWAIHRDPQLWDDPMSFKPE 420
Query: 421 RHATLTIEDQYKYLPFGLGRRACPGGGMANRLVGLALATMIQCFEWKRVSDELVDMTVGN 480
R + K +PFGLGRRACPG G+A+RL+ L L ++IQC EW+++ +E VDM+ G
Sbjct: 421 RFE--KEGEAQKLMPFGLGRRACPGSGLAHRLINLTLGSLIQCLEWEKIGEE-VDMSEGK 480
Query: 481 GITASRAFPLEAMCKPWPDMERVLVEEV 506
G+T +A PLEAMC+ P + ++ E V
Sbjct: 481 GVTMPKAKPLEAMCRARPSVVKIFNESV 497
BLAST of MC02g_new0213 vs. TAIR 10
Match:
AT4G37340.1 (cytochrome P450, family 81, subfamily D, polypeptide 3 )
HSP 1 Score: 474.2 bits (1219), Expect = 1.3e-133
Identity = 247/508 (48.62%), Postives = 339/508 (66.73%), Query Frame = 0
Query: 1 MENILLYFSLSLLSILVVRFLLRSPSGRKLPPSPAFRLPIIGHLHLLGRPAQHAFKKLAD 60
ME L F+ +S+ + + R LPPSP++ LP+IGHL LL P F +++
Sbjct: 1 MEGQTLIFTFLFISLSLTFIIGRIKRRPNLPPSPSWALPVIGHLRLLKPPLHRVFLSVSE 60
Query: 61 KYG--PVFSLRFGSRLVVIITSPEAVKECFTKNDVVLAARPLLNSGKYMAYDNTIFAAAS 120
G P+ SLR G+RLV +++S +ECFTKNDVVLA R + K+++Y T AS
Sbjct: 61 SLGDAPIISLRLGNRLVFVVSSHSLAEECFTKNDVVLANRFNSLASKHISYGCTTVVTAS 120
Query: 121 YGEHWRNLRRIGSIEIYSPTRLNKFLDSRGDEVKRLLRRLGESNNSPPPASAGFSTVKLE 180
YG+HWRNLRRIG++EI+S RLN F R DE+ RL+ L S NS S F+ V+++
Sbjct: 121 YGDHWRNLRRIGAVEIFSAHRLNSFSSIRRDEIHRLIACL--SRNS----SLEFTKVEMK 180
Query: 181 PLFAELTFNVVMRMVAGKSYFGENVSNEEEAKQFRYIMKEIFMISEASSPGDFIPIWNWI 240
+F+ LTFN ++RM+AGK Y+G+ ++ EAK+ R ++ E A + D++PI WI
Sbjct: 181 SMFSNLTFNNIIRMLAGKCYYGDGAEDDPEAKRVRELIAEGMGCFGAGNTADYLPILTWI 240
Query: 241 DPTGYEKKLKNIAKKVDIFLVNLIEEIRNRKGE-GNTMVDHLLSMQVSEPQFFSDQIIKG 300
TG EK++K IA ++D FL L++E R K + NTMVDHLL +Q ++P++++D IIKG
Sbjct: 241 --TGSEKRIKKIASRLDEFLQGLVDERREGKEKRQNTMVDHLLCLQETQPEYYTDNIIKG 300
Query: 301 LIWVIMLAGTDTQSGTLTWTMSHLLNNPEVLKKARAEIDTVVGRERLVEENDLSKMTYLQ 360
++ ++LAGTDT + TL WT+S LLN+P++L KAR EID VG RLVEE+DLS + YLQ
Sbjct: 301 IMLSLILAGTDTSAVTLEWTLSALLNHPQILSKARDEIDNKVGLNRLVEESDLSHLPYLQ 360
Query: 361 QVIAETLRLNPL-PLLLPHCASEDCTIQGYHIPRDTMLLFNVWAIHRDPKLWDDALIFKP 420
+++E+LRL P PLL+PH ASEDC + GYH+PR TMLL N WAIHRDPK+WDD FKP
Sbjct: 361 NIVSESLRLYPASPLLVPHVASEDCKVGGYHMPRGTMLLTNAWAIHRDPKIWDDPTSFKP 420
Query: 421 ERHATLTIEDQYKYLPFGLGRRACPGGGMANRLVGLALATMIQCFEWKRVSDELVDMTV- 480
ER + K L FGLGRRACPG G+A RL L + ++IQCFEW+R+ +E VDMT
Sbjct: 421 ERFE--KEGEAQKLLGFGLGRRACPGSGLAQRLASLTIGSLIQCFEWERIGEEEVDMTEG 480
Query: 481 GNGITASRAFPLEAMCKPWPDMERVLVE 504
G G+ +A PL AMCK P + ++L E
Sbjct: 481 GGGVIMPKAIPLVAMCKARPVVGKILNE 498
BLAST of MC02g_new0213 vs. TAIR 10
Match:
AT4G37360.1 (cytochrome P450, family 81, subfamily D, polypeptide 2 )
HSP 1 Score: 473.0 bits (1216), Expect = 3.0e-133
Identity = 237/496 (47.78%), Postives = 337/496 (67.94%), Query Frame = 0
Query: 1 MENILLYFSLSLLSILVVRFLLRSPSGRKLPPSPAFRLPIIGHLHLLGRPAQHAFKKLAD 60
ME ++L F+ + + ++ + R LPPSPA+ LP+IGHL LL P F ++
Sbjct: 1 MEALMLIFTFCFIVLSLIFLIGRIKRKLNLPPSPAWALPVIGHLRLLKPPLHRVFLSVSQ 60
Query: 61 KYG--PVFSLRFGSRLVVIITSPEAVKECFTKNDVVLAARPLLNSGKYMAYDNTIFAAAS 120
G P+ SLR G+RL+ +++S +ECFTKNDV+LA R S K+++Y N+ +AS
Sbjct: 61 SLGDAPIISLRLGNRLLFVVSSHSIAEECFTKNDVILANRQTTISTKHISYGNSTVVSAS 120
Query: 121 YGEHWRNLRRIGSIEIYSPTRLNKFLDSRGDEVKRLLRRLGESNNSPPPASAGFSTVKLE 180
Y EHWRNLRRIG++EI+S RLN F R DE++RL+ RL + +S GF+ V+++
Sbjct: 121 YSEHWRNLRRIGALEIFSAHRLNSFSSIRRDEIRRLIGRLLRN------SSYGFTKVEMK 180
Query: 181 PLFAELTFNVVMRMVAGKSYFGENVSNEEEAKQFRYIMKEIFMISEASSPGDFIPIWNWI 240
+F++LTFN ++RM+AGK Y+G+ ++ EAK+ R ++ E S + D+IPI WI
Sbjct: 181 SMFSDLTFNNIIRMLAGKCYYGDGKEDDPEAKRVRTLIAEAMSSSGPGNAADYIPILTWI 240
Query: 241 DPTGYEKKLKNIAKKVDIFLVNLIEEIR-NRKGEGNTMVDHLLSMQVSEPQFFSDQIIKG 300
T E ++K +A ++D FL L++E R ++ + NTMVDHLL +Q ++P+++ D+IIKG
Sbjct: 241 --TYSETRIKKLAGRLDEFLQGLVDEKREGKEKKENTMVDHLLCLQETQPEYYMDRIIKG 300
Query: 301 LIWVIMLAGTDTQSGTLTWTMSHLLNNPEVLKKARAEIDTVVGRERLVEENDLSKMTYLQ 360
+ ++ GTDT + TL W +S LLNNPEVL KAR EID ++G +RL+EE+D+ + YLQ
Sbjct: 301 TMLSLIAGGTDTTAVTLEWALSSLLNNPEVLNKARDEIDRMIGVDRLLEESDIPNLPYLQ 360
Query: 361 QVIAETLRLNP-LPLLLPHCASEDCTIQGYHIPRDTMLLFNVWAIHRDPKLWDDALIFKP 420
+++ETLRL P P+LLPH AS+DC + GY +PR TMLL N WAIHRDP LWDD FKP
Sbjct: 361 NIVSETLRLYPAAPMLLPHVASKDCKVGGYDMPRGTMLLTNAWAIHRDPLLWDDPTSFKP 420
Query: 421 ERHATLTIEDQYKYLPFGLGRRACPGGGMANRLVGLALATMIQCFEWKRVSDELVDMTVG 480
ER + K +PFGLGRRACPG G+A RLV L+L ++IQCFEW+R+ +E VDMT G
Sbjct: 421 ERFE--KEGEAKKLMPFGLGRRACPGSGLAQRLVTLSLGSLIQCFEWERIGEEEVDMTEG 480
Query: 481 NGITASRAFPLEAMCK 493
G+T +A PLEAMC+
Sbjct: 481 PGLTMPKARPLEAMCR 486
BLAST of MC02g_new0213 vs. TAIR 10
Match:
AT4G37330.1 (cytochrome P450, family 81, subfamily D, polypeptide 4 )
HSP 1 Score: 472.6 bits (1215), Expect = 3.9e-133
Identity = 237/493 (48.07%), Postives = 325/493 (65.92%), Query Frame = 0
Query: 3 NILLYFSLSLLSILVVRFLLRSPSGRKLPPSPAFRLPIIGHLHLLGRPAQHAFKKLADKY 62
N +L S L L + L RS LPPSPA LP+IGHLHLL P F L+
Sbjct: 4 NQILILSFLSLFTLAIFLLTRSKRKLNLPPSPAISLPVIGHLHLLKPPLHRTFLSLSKSI 63
Query: 63 G--PVFSLRFGSRLVVIITSPEAVKECFTKNDVVLAARPLLNSGKYMAYDNTIFAAASYG 122
G PVF LR G+RLV +I+S +ECFTKNDVVLA RP K++ Y+ T +ASYG
Sbjct: 64 GNAPVFHLRLGNRLVYVISSRSIAEECFTKNDVVLANRPKFTISKHLGYNATYLLSASYG 123
Query: 123 EHWRNLRRIGSIEIYSPTRLNKFLDSRGDEVKRLLRRLGESNNSPPPASAGFSTVKLEPL 182
+HWRNLRRI ++EI+S RLN FL R DE++RL+ L + GF V+++ L
Sbjct: 124 DHWRNLRRIAAVEIFSTHRLNSFLYIRKDEIRRLISHLSRD------SLHGFVEVEMKTL 183
Query: 183 FAELTFNVVMRMVAGKSYFGENVSNEEEAKQFRYIMKEIFMISEASSPGDFIPIWNWIDP 242
L N +RM+AGK YFGE + ++AK + ++ E + A +P D++ I W+
Sbjct: 184 LTNLASNTTIRMLAGKRYFGE---DNDDAKLVKNLVSEAVTSAGAGNPIDYLSILRWV-- 243
Query: 243 TGYEKKLKNIAKKVDIFLVNLIEEIRNRKGEGNTMVDHLLSMQVSEPQFFSDQIIKGLIW 302
+ YEK++KN+ + D FL L++E R K +G TM+DHLL++Q +P +++D IIKG+I
Sbjct: 244 SSYEKRIKNLGNRFDTFLQKLVDEKRAEKEKGETMIDHLLALQDIQPDYYTDVIIKGIIL 303
Query: 303 VIMLAGTDTQSGTLTWTMSHLLNNPEVLKKARAEIDTVVGRERLVEENDLSKMTYLQQVI 362
+++AGTDT S TL W MS+LLN+PE+LKKAR EID VG +RLV+E+D+ ++YLQ ++
Sbjct: 304 TLIIAGTDTSSVTLEWAMSNLLNHPEILKKARMEIDEKVGLDRLVDESDIVNLSYLQSIV 363
Query: 363 AETLRLNP-LPLLLPHCASEDCTIQGYHIPRDTMLLFNVWAIHRDPKLWDDALIFKPERH 422
ETLR+ P +PLLLPH +SEDC + GY IP TM+L N WA+HRDP++W+D IFKPER
Sbjct: 364 LETLRMYPAVPLLLPHLSSEDCKVGGYDIPSGTMVLTNAWAMHRDPEVWEDPEIFKPERF 423
Query: 423 ATLTIEDQYKYLPFGLGRRACPGGGMANRLVGLALATMIQCFEWKRVSDELVDMTVGNGI 482
+ K + FG+GRRACPG G+A+RL+ AL +++QCFEW+RV ++ VDMT G
Sbjct: 424 E--KEGEAEKLISFGMGRRACPGAGLAHRLINQALGSLVQCFEWERVGEDFVDMTEDKGA 483
Query: 483 TASRAFPLEAMCK 493
T +A PL AMCK
Sbjct: 484 TLPKAIPLRAMCK 483
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| W8JMU7 | 5.5e-148 | 51.41 | Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1 | [more] |
| Q6WNQ8 | 1.9e-140 | 51.22 | Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1 | [more] |
| Q6WNQ9 | 5.5e-140 | 51.50 | Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 P... | [more] |
| Q6WNR0 | 2.7e-139 | 49.50 | Isoflavone 2'-hydroxylase OS=Medicago truncatula OX=3880 GN=CYP81E7 PE=1 SV=1 | [more] |
| Q9LHA1 | 1.7e-136 | 49.51 | Cytochrome P450 81D11 OS=Arabidopsis thaliana OX=3702 GN=CYP81D11 PE=2 SV=1 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A5K7U6A3 | 0.0 | 100.00 | Cytochrome P450 monooxygenase OS=Momordica charantia OX=3673 GN=McCYP81AQ19 PE=2... | [more] |
| A0A6J1D5S6 | 8.51e-211 | 58.53 | isoflavone 2'-hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111017884 PE... | [more] |
| A0A6J1D5S1 | 4.42e-206 | 57.99 | cytochrome P450 81E8-like OS=Momordica charantia OX=3673 GN=LOC111017881 PE=3 SV... | [more] |
| A0A7N2R7H8 | 3.25e-205 | 57.43 | Uncharacterized protein OS=Quercus lobata OX=97700 PE=3 SV=1 | [more] |
| A0A6J1FUI0 | 7.50e-205 | 57.62 | cytochrome P450 81D1-like OS=Cucurbita moschata OX=3662 GN=LOC111448921 PE=3 SV=... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| AT3G28740.1 | 1.2e-137 | 49.51 | Cytochrome P450 superfamily protein | [more] |
| AT4G37370.1 | 1.2e-137 | 47.64 | cytochrome P450, family 81, subfamily D, polypeptide 8 | [more] |
| AT4G37340.1 | 1.3e-133 | 48.62 | cytochrome P450, family 81, subfamily D, polypeptide 3 | [more] |
| AT4G37360.1 | 3.0e-133 | 47.78 | cytochrome P450, family 81, subfamily D, polypeptide 2 | [more] |
| AT4G37330.1 | 3.9e-133 | 48.07 | cytochrome P450, family 81, subfamily D, polypeptide 4 | [more] |
