MC02g_new0189 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC02g_new0189
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	disease resistance protein RGA2-like
Location	MC02: 8521759 .. 8523780 (+)
RNA-Seq Expression	MC02g_new0189
Synteny	MC02g_new0189

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CACCGTGCCAACTACCAAGTCCACGTTTACTTTCAAAGACAACTTTGATTTATAAACCCTATGTATTTTGGATCTTCTTCCCCAAATCCACTGCAATTTTCAGGAAAACTATAATCAGAGCTATGGCTGAGTTGCTATTGACTTTTGCTGTAGAGGGAGTGTTAAGCAAAGTGGTGACAGTTGCAACTGAGCAAATTGGGCTCGCATGGGGCCTGGAGAAGGAGCTAGAGGAGCTCAAGAAATGGCTACTCAACATTGAAGCCTTCTTATGTGATATCAACAAAAGCAAATTTCATCATAATTCAGTGGAGCTGTGGGTGGACAAACTTCAAGATCTTGTTCATGAAGCTGAGAATCCATTGGACGAGCTTGTTTATGAGCATCTTCGGCGAAAGGTGCAAACAGGAAAAAGGAACAAGGTACGTGATTTTGCATCCTCTTCTAATAATCCTTTGGTCTTTCCTGTCAAGATGGCAAATAGAATAAAGAACATTACGAACCAGTTGCAAGAGCATTATGGTAGGGCAGGTCATTTGGGAATTGTAGGCAGTGGATCCACAGGAACAGAGACTGTTATTAGCCAAATTCGAGAGACAGTCTCGGAGTTGGATGACTTTGGAGTCGTGGGGAGGGATGTTGAAGTCTCTAGCATAGTGAAGCAGGTGGTGGATGCTACAAATAGTCATGTTACATCTATCTTACCCATTGTGGGTGTGGGTGGGCTGGGAAAAACAACGTTGGCAAAGTTGGTCTTCAATCACCCAAGGATCAAACAACATTTTGATAAAACTATATGGGTGTGTGTCCGAACCTTTTGATATCAACAAGATTTTGGAAGCAATCTTACAAAACTTAAAAGGTCCTTCAGGCGCCTGCGATAACAGGGAGGTATTGCTTCATGAACTTAAAAAGGAAATGCGTGACAAAAGATATTTTCTTGTGCTTGATGACGTTTGGAATGAAAATTCTTCTTTGTGGGATGAGTTGAAGAATTGTTTAATGAGGATGACCGAAAAATCAGAAAATAGTATTGTTGTTACTACACGCAACGTTGAAGTTGCCAAAATCATAGAAACACTTCCTAGTCATCATTTGAGTAAGCTCTCTATCGATCAGTTTTGGTCCTTATTTCAGGAAAGTGCAAATGCAAATGGACTACCAATGACTTTAAGTTTGGAAATTGTTCGAGAAAGGTTGATTAAAAAAATTGGTGGCACACTATTAGTGGCTAAAGTTTGGGGAAAGACAGTACAATTTCAAGGAAACTATGATGGATGGGTGACGAAAATTGGAGAGTGAAATAAATGAAATGAGAACTCCAACTCAAGAGGAAAAATGTGTTTTAGAAATATTGAAATTAAGTGTGGATCGCCTGCCATCATTTTTATTAAAGCAATGTTTTGCCTATACTGTTCAAATTTTTCCAAAGATTTTGTGTTTGAAAAAGAACAACTAATTCTTACGTGGATAGCACAAGGATTTATTCATCTACAAGAAAGAAGTAGCAACGAAACAATGGAAGACGAAGGAGATAGGCACTTCAAGATTTTGTTGTCTCGTTGCTTATTTCAAGATGTTGTTAAAGATAGCACAGGAAGAATTATAAGATGTAAGATGCATGATCTTTTACATAAAATTGCATGTGATGTCTCAAATGATCTTAAATTACAATATTTTATCAATTCATTGGATGCGGAATATTGGACGAATGAGAATCAAAACATTGCAAGCAAGTTATGCACCATAATTATTTATGATCAAAAGATGATGCCTTACAAGCAAGGGGAGGTGATTCGTGATAAGATGATAAACTTTGTTTGTTTACGTGTTTTGGTGATGAATTTGAAGTCTATCTATGAGTTACCAGATTCAATTGGTAAGTTGAAACACCTGAGATATCTCGACATTTCAAATTGTTCAATAAAAAAGCTTCCAGAATCTATTGGCGTGCTTTATAATTTGCAAACACTAAGGATGACATGGACGCCATCGGTAGATTTTCCAAAGAATTTTGGAAAATTA

mRNA sequence

ATGCTACAAATAGTCATGTTACATCTATCTTACCCATTGTGGGTGTGGGTGGGCTGGGAAAAACAACGTTGGCAAAGTTGGTCTTCAATCACCCAAGGATCAAACAACATTTTGATAAAACTATATGGGTGTGTGTCCGAACCTTTTGATATCAACAAGATTTTGGAAGCAATCTTACAAAACTTAAAAGGTCCTTCAGGCGCCTGCGATAACAGGGAGGTATTGCTTCATGAACTTAAAAAGGAAATGCGTGACAAAAGATATTTTCTTGTGCTTGATGACGTTTGGAATGAAAATTCTTCTTTGTGGGATGAGTTGAAGAATTGTTTAATGAGGATGACCGAAAAATCAGAAAATAGTATTGTTGTTACTACACGCAACGTTGAAGTTGCCAAAATCATAGAAACACTTCCTAGTCATCATTTGAGTAAGCTCTCTATCGATCAGTTTTGGTCCTTATTTCAGGAAAGTGCAAATGCAAATGGACTACCAATGACTTTAAGTTTGGAAATTGTTCGAGAAAGGTTGATTAAAAAAATTGGTGGCACACTATTAGTGGCTAAAGTTTGGGGAAAGACAGTACAATTTCAAGGAAACTATGATGGATGGGTGACGAAAATTGGAGAGTGA

Coding sequence (CDS)

ATGCTACAAATAGTCATGTTACATCTATCTTACCCATTGTGGGTGTGGGTGGGCTGGGAAAAACAACGTTGGCAAAGTTGGTCTTCAATCACCCAAGGATCAAACAACATTTTGATAAAACTATATGGGTGTGTGTCCGAACCTTTTGATATCAACAAGATTTTGGAAGCAATCTTACAAAACTTAAAAGGTCCTTCAGGCGCCTGCGATAACAGGGAGGTATTGCTTCATGAACTTAAAAAGGAAATGCGTGACAAAAGATATTTTCTTGTGCTTGATGACGTTTGGAATGAAAATTCTTCTTTGTGGGATGAGTTGAAGAATTGTTTAATGAGGATGACCGAAAAATCAGAAAATAGTATTGTTGTTACTACACGCAACGTTGAAGTTGCCAAAATCATAGAAACACTTCCTAGTCATCATTTGAGTAAGCTCTCTATCGATCAGTTTTGGTCCTTATTTCAGGAAAGTGCAAATGCAAATGGACTACCAATGACTTTAAGTTTGGAAATTGTTCGAGAAAGGTTGATTAAAAAAATTGGTGGCACACTATTAGTGGCTAAAGTTTGGGGAAAGACAGTACAATTTCAAGGAAACTATGATGGATGGGTGACGAAAATTGGAGAGTGA

Protein sequence

MLQIVMLHLSYPLWVWVGWEKQRWQSWSSITQGSNNILIKLYGCVSEPFDINKILEAILQNLKGPSGACDNREVLLHELKKEMRDKRYFLVLDDVWNENSSLWDELKNCLMRMTEKSENSIVVTTRNVEVAKIIETLPSHHLSKLSIDQFWSLFQESANANGLPMTLSLEIVRERLIKKIGGTLLVAKVWGKTVQFQGNYDGWVTKIGE

Homology

[Close]

BLAST of MC02g_new0189 vs. ExPASy Swiss-Prot
Match: Q7XA40 (Putative disease resistance protein RGA3 OS=Solanum bulbocastanum OX=147425 GN=RGA3 PE=2 SV=2)

HSP 1 Score: 102.4 bits (254), Expect = 6.2e-21
Identity = 57/165 (34.55%), Postives = 95/165 (57.58%), Query Frame = 0


Query: 39  IKLYGCVSEPFDINKILEAILQNLKGPSGACDNREVLLHELKKEMRDKRYFLVLDDVWNE 98
           +K++ CVS+ FD  ++++AI+++++G S    +   L  +L++ +  KRYFLVLDDVWNE
Sbjct: 207 LKIWVCVSDDFDEKRLIKAIVESIEGKSLGDMDLAPLQKKLQELLNGKRYFLVLDDVWNE 266

Query: 99  NSSLWDELKNCLMRMTEKSENSIVVTTRNVEVAKIIETLPSHHLSKLSIDQFWSLFQESA 158
           +   WD L+  L      S  SI++TTR  ++  I+ TL  + LS LS +  W LF++ A
Sbjct: 267 DQEKWDNLRAVL--KIGASGASILITTRLEKIGSIMGTLQLYQLSNLSQEDCWLLFKQRA 326

Query: 159 NANGLPMTLSLEIVRERLIKKIGGTLLVAKVWGKTVQFQGNYDGW 204
             +    +  L  + + ++KK GG  L AK  G  ++F+     W
Sbjct: 327 FCHQTETSPKLMEIGKEIVKKCGGVPLAAKTLGGLLRFKREESEW 369

[Close]

BLAST of MC02g_new0189 vs. ExPASy Swiss-Prot
Match: Q7XA42 (Putative disease resistance protein RGA1 OS=Solanum bulbocastanum OX=147425 GN=RGA1 PE=2 SV=2)

HSP 1 Score: 92.8 bits (229), Expect = 4.9e-18
Identity = 53/164 (32.32%), Postives = 92/164 (56.10%), Query Frame = 0


Query: 40  KLYGCVSEPFDINKILEAILQNLKGPSGACDNREVLLHELKKEMRDKRYFLVLDDVWNEN 99
           K++ C+S+ F+  ++++AI+++++G S +  +   L  +L++ +  KRYFLVLDDVWNE+
Sbjct: 208 KIWICISDDFNEKRLIKAIVESIEGKSLSDMDLAPLQKKLQELLNGKRYFLVLDDVWNED 267

Query: 100 SSLWDELKNCLMRMTEKSENSIVVTTRNVEVAKIIETLPSHHLSKLSIDQFWSLFQESAN 159
              W  L+  L      S   ++ TTR  +V  I+ TL  + LS LS +  W LF + A 
Sbjct: 268 QHKWANLRAVL--KVGASGAFVLTTTRLEKVGSIMGTLQPYELSNLSPEDCWFLFMQRAF 327

Query: 160 ANGLPMTLSLEIVRERLIKKIGGTLLVAKVWGKTVQFQGNYDGW 204
            +   +  +L  + + ++KK GG  L AK  G  ++F+     W
Sbjct: 328 GHQEEINPNLMAIGKEIVKKCGGVPLAAKTLGGILRFKREEREW 369

[Close]

BLAST of MC02g_new0189 vs. ExPASy Swiss-Prot
Match: Q7XBQ9 (Disease resistance protein RGA2 OS=Solanum bulbocastanum OX=147425 GN=RGA2 PE=1 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 1.9e-17
Identity = 56/165 (33.94%), Postives = 90/165 (54.55%), Query Frame = 0


Query: 40  KLYGCVSEPFDINKILEAILQNLKG-PSGACDNREVLLHELKKEMRDKRYFLVLDDVWNE 99
           K++ CVSE FD  ++++AI+++++G P     +   L  +L++ +  KRY LVLDDVWNE
Sbjct: 208 KIWICVSEDFDEKRLIKAIVESIEGRPLLGEMDLAPLQKKLQELLNGKRYLLVLDDVWNE 267

Query: 100 NSSLWDELKNCLMRMTEKSENSIVVTTRNVEVAKIIETLPSHHLSKLSIDQFWSLFQESA 159
           +   W  L+  L      S  S++ TTR  +V  I+ TL  + LS LS +  W LF + A
Sbjct: 268 DQQKWANLRAVL--KVGASGASVLTTTRLEKVGSIMGTLQPYELSNLSQEDCWLLFMQRA 327

Query: 160 NANGLPMTLSLEIVRERLIKKIGGTLLVAKVWGKTVQFQGNYDGW 204
             +   +  +L  + + ++KK GG  L AK  G  + F+     W
Sbjct: 328 FGHQEEINPNLVAIGKEIVKKSGGVPLAAKTLGGILCFKREERAW 370

[Close]

BLAST of MC02g_new0189 vs. ExPASy Swiss-Prot
Match: Q7XA39 (Putative disease resistance protein RGA4 OS=Solanum bulbocastanum OX=147425 GN=RGA4 PE=2 SV=1)

HSP 1 Score: 87.0 bits (214), Expect = 2.7e-16
Identity = 52/164 (31.71%), Postives = 86/164 (52.44%), Query Frame = 0


Query: 40  KLYGCVSEPFDINKILEAILQNLKGPSGACDNREVLLHELKKEMRDKRYFLVLDDVWNEN 99
           K++ CVS+ FD  ++++ I+ N++  S   ++      +L++ +  KRY LVLDDVWN++
Sbjct: 210 KIWVCVSDDFDEKRLIKTIIGNIERSSPHVEDLASFQKKLQELLNGKRYLLVLDDVWNDD 269

Query: 100 SSLWDELKNCLMRMTEKSENSIVVTTRNVEVAKIIETLPSHHLSKLSIDQFWSLFQESAN 159
              W +L+  L      +  SI+ TTR  +V  I+ TL  +HLS LS      LF + A 
Sbjct: 270 LEKWAKLRAVLTVGARGA--SILATTRLEKVGSIMGTLQPYHLSNLSPHDSLLLFMQRAF 329

Query: 160 ANGLPMTLSLEIVRERLIKKIGGTLLVAKVWGKTVQFQGNYDGW 204
                   +L  + + ++KK GG  L AK  G  ++F+     W
Sbjct: 330 GQQKEANPNLVAIGKEIVKKCGGVPLAAKTLGGLLRFKREESEW 371

[Close]

BLAST of MC02g_new0189 vs. ExPASy Swiss-Prot
Match: Q9LRR4 (Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=RPPL1 PE=3 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 5.0e-15
Identity = 56/168 (33.33%), Postives = 90/168 (53.57%), Query Frame = 0


Query: 40  KLYGCVSEPFDINKILEAILQNLKGPSGACDNREVLLHELKKEMRDK--RYFLVLDDVWN 99
           K++  VSE FD+ KI + + +++        + +VL  +LK+ +      + LVLDD+WN
Sbjct: 229 KVWAHVSEEFDVFKITKKVYESVTSRPCEFTDLDVLQVKLKERLTGTGLPFLLVLDDLWN 288

Query: 100 ENSSLWDELKNCLMRMTEKSENSIVVTTRNVEVAKIIETLPSHHLSKLSIDQFWSLFQES 159
           EN + WD L+   +   + S+  I+VTTR+  VA I+  +  H+L  LS    WSLF ++
Sbjct: 289 ENFADWDLLRQPFIHAAQGSQ--ILVTTRSQRVASIMCAVHVHNLQPLSDGDCWSLFMKT 348

Query: 160 ANANGLPMTLSLEI--VRERLIKKIGGTLLVAKVWGKTVQFQGNYDGW 204
              N  P  L+ EI  + ER++ K  G  L  K  G  ++F+G    W
Sbjct: 349 VFGNQEP-CLNREIGDLAERIVHKCRGLPLAVKTLGGVLRFEGKVIEW 393

[Close]

BLAST of MC02g_new0189 vs. NCBI nr
Match: XP_022150842.1 (putative disease resistance protein RGA3 [Momordica charantia])

HSP 1 Score: 246 bits (628), Expect = 5.58e-74
Identity = 122/165 (73.94%), Postives = 142/165 (86.06%), Query Frame = 0


Query: 41  LYGCVSEPFDINKILEAILQNLKGPSGACDNREVLLHELKKEMRDKRYFLVLDDVWNENS 100
           ++ CVSEPF +NKIL AIL+ L+G S   DN+EVLL ELKKEM  KRYFLVLDDVWNEN 
Sbjct: 224 IWVCVSEPFLVNKILGAILETLRGTSDRLDNKEVLLCELKKEMHGKRYFLVLDDVWNENP 283

Query: 101 SLWDELKNCLMRMTEKSENSIVVTTRNVEVAKIIETLPSHHLSKLSIDQFWSLFQESANA 160
           SLWDELKNCL R+TE+SENSI+VTTR++EVAKI+ETLPS+HLSKLS DQ W LFQESANA
Sbjct: 284 SLWDELKNCLTRITEESENSILVTTRSIEVAKIMETLPSYHLSKLSDDQCWYLFQESANA 343

Query: 161 NGLPMTLSLEIVRERLIKKIGGTLLVAKVWGKTVQFQGNYDGWVT 205
           NGLPMT SL++ +E L+KKIGG  LVAKV G+TV+FQGN+D WVT
Sbjct: 344 NGLPMTSSLKVFQEGLVKKIGGVPLVAKVLGRTVKFQGNFDIWVT 388

[Close]

BLAST of MC02g_new0189 vs. NCBI nr
Match: XP_038901403.1 (putative disease resistance protein RGA3 isoform X3 [Benincasa hispida])

HSP 1 Score: 240 bits (613), Expect = 3.29e-73
Identity = 119/167 (71.26%), Postives = 137/167 (82.04%), Query Frame = 0


Query: 41  LYGCVSEPFDINKILEAILQNLKGPSGACDNREVLLHELKKEMRDKRYFLVLDDVWNENS 100
           ++ CVSEPF INKIL  IL  LKG S   DN+E LL ELK EMR K YFLVLDDVWNEN 
Sbjct: 108 IWVCVSEPFLINKILGTILVTLKGTSSGLDNKEALLRELKYEMRHKSYFLVLDDVWNENP 167

Query: 101 SLWDELKNCLMRMTEKSENSIVVTTRNVEVAKIIETLPSHHLSKLSIDQFWSLFQESANA 160
           SLWDELKNCLM++TEKSENS VVTTR+VEVA+I+ETLP+HHLSKLS DQ WSLFQ S+NA
Sbjct: 168 SLWDELKNCLMKITEKSENSFVVTTRSVEVARIMETLPTHHLSKLSDDQCWSLFQASSNA 227

Query: 161 NGLPMTLSLEIVRERLIKKIGGTLLVAKVWGKTVQFQGNYDGWVTKI 207
            GLPMT SL ++RE L+KKIGG  LVA+V G+ ++FQGNYD WVT +
Sbjct: 228 IGLPMTSSLTLLREELVKKIGGIPLVARVLGRALKFQGNYDRWVTML 274

[Close]

BLAST of MC02g_new0189 vs. NCBI nr
Match: XP_038901402.1 (putative disease resistance protein RGA3 isoform X2 [Benincasa hispida])

HSP 1 Score: 240 bits (613), Expect = 4.82e-72
Identity = 119/167 (71.26%), Postives = 137/167 (82.04%), Query Frame = 0


Query: 41  LYGCVSEPFDINKILEAILQNLKGPSGACDNREVLLHELKKEMRDKRYFLVLDDVWNENS 100
           ++ CVSEPF INKIL  IL  LKG S   DN+E LL ELK EMR K YFLVLDDVWNEN 
Sbjct: 108 IWVCVSEPFLINKILGTILVTLKGTSSGLDNKEALLRELKYEMRHKSYFLVLDDVWNENP 167

Query: 101 SLWDELKNCLMRMTEKSENSIVVTTRNVEVAKIIETLPSHHLSKLSIDQFWSLFQESANA 160
           SLWDELKNCLM++TEKSENS VVTTR+VEVA+I+ETLP+HHLSKLS DQ WSLFQ S+NA
Sbjct: 168 SLWDELKNCLMKITEKSENSFVVTTRSVEVARIMETLPTHHLSKLSDDQCWSLFQASSNA 227

Query: 161 NGLPMTLSLEIVRERLIKKIGGTLLVAKVWGKTVQFQGNYDGWVTKI 207
            GLPMT SL ++RE L+KKIGG  LVA+V G+ ++FQGNYD WVT +
Sbjct: 228 IGLPMTSSLTLLREELVKKIGGIPLVARVLGRALKFQGNYDRWVTML 274

[Close]

BLAST of MC02g_new0189 vs. NCBI nr
Match: XP_038901401.1 (disease resistance protein RGA2-like isoform X1 [Benincasa hispida])

HSP 1 Score: 240 bits (613), Expect = 6.99e-72
Identity = 119/167 (71.26%), Postives = 137/167 (82.04%), Query Frame = 0


Query: 41  LYGCVSEPFDINKILEAILQNLKGPSGACDNREVLLHELKKEMRDKRYFLVLDDVWNENS 100
           ++ CVSEPF INKIL  IL  LKG S   DN+E LL ELK EMR K YFLVLDDVWNEN 
Sbjct: 108 IWVCVSEPFLINKILGTILVTLKGTSSGLDNKEALLRELKYEMRHKSYFLVLDDVWNENP 167

Query: 101 SLWDELKNCLMRMTEKSENSIVVTTRNVEVAKIIETLPSHHLSKLSIDQFWSLFQESANA 160
           SLWDELKNCLM++TEKSENS VVTTR+VEVA+I+ETLP+HHLSKLS DQ WSLFQ S+NA
Sbjct: 168 SLWDELKNCLMKITEKSENSFVVTTRSVEVARIMETLPTHHLSKLSDDQCWSLFQASSNA 227

Query: 161 NGLPMTLSLEIVRERLIKKIGGTLLVAKVWGKTVQFQGNYDGWVTKI 207
            GLPMT SL ++RE L+KKIGG  LVA+V G+ ++FQGNYD WVT +
Sbjct: 228 IGLPMTSSLTLLREELVKKIGGIPLVARVLGRALKFQGNYDRWVTML 274

[Close]

BLAST of MC02g_new0189 vs. NCBI nr
Match: XP_022158717.1 (putative disease resistance protein RGA3 [Momordica charantia])

HSP 1 Score: 235 bits (600), Expect = 1.73e-68
Identity = 116/167 (69.46%), Postives = 140/167 (83.83%), Query Frame = 0


Query: 41  LYGCVSEPFDINKILEAILQNLKGPSGACDNREVLLHELKKEMRDKRYFLVLDDVWNENS 100
           ++ CVSEPF +NKIL AIL  L+G S   DN+E LLHELKKEM  KRYFLVLDDVWNE+ 
Sbjct: 224 IWVCVSEPFLVNKILGAILGTLRGTSSGLDNKEALLHELKKEMHGKRYFLVLDDVWNESH 283

Query: 101 SLWDELKNCLMRMTEKSENSIVVTTRNVEVAKIIETLPSHHLSKLSIDQFWSLFQESANA 160
           SLWDELKNCLMR+TE+SENSI+VTTR+ EVAKI+ETLP+HHLSKLS DQ WSLF ESAN 
Sbjct: 284 SLWDELKNCLMRITERSENSILVTTRSAEVAKIMETLPNHHLSKLSDDQCWSLFLESANV 343

Query: 161 NGLPMTLSLEIVRERLIKKIGGTLLVAKVWGKTVQFQGNYDGWVTKI 207
           NGL +T SL++V+E L+KKIGG  LVA+V G+ V+F+G+Y+ WVT +
Sbjct: 344 NGLSITSSLKVVQE-LVKKIGGIPLVARVLGRAVKFEGDYETWVTTL 389

[Close]

BLAST of MC02g_new0189 vs. ExPASy TrEMBL
Match: A0A6J1DBV6 (putative disease resistance protein RGA3 OS=Momordica charantia OX=3673 GN=LOC111018890 PE=4 SV=1)

HSP 1 Score: 246 bits (628), Expect = 2.70e-74
Identity = 122/165 (73.94%), Postives = 142/165 (86.06%), Query Frame = 0


Query: 41  LYGCVSEPFDINKILEAILQNLKGPSGACDNREVLLHELKKEMRDKRYFLVLDDVWNENS 100
           ++ CVSEPF +NKIL AIL+ L+G S   DN+EVLL ELKKEM  KRYFLVLDDVWNEN 
Sbjct: 224 IWVCVSEPFLVNKILGAILETLRGTSDRLDNKEVLLCELKKEMHGKRYFLVLDDVWNENP 283

Query: 101 SLWDELKNCLMRMTEKSENSIVVTTRNVEVAKIIETLPSHHLSKLSIDQFWSLFQESANA 160
           SLWDELKNCL R+TE+SENSI+VTTR++EVAKI+ETLPS+HLSKLS DQ W LFQESANA
Sbjct: 284 SLWDELKNCLTRITEESENSILVTTRSIEVAKIMETLPSYHLSKLSDDQCWYLFQESANA 343

Query: 161 NGLPMTLSLEIVRERLIKKIGGTLLVAKVWGKTVQFQGNYDGWVT 205
           NGLPMT SL++ +E L+KKIGG  LVAKV G+TV+FQGN+D WVT
Sbjct: 344 NGLPMTSSLKVFQEGLVKKIGGVPLVAKVLGRTVKFQGNFDIWVT 388

[Close]

BLAST of MC02g_new0189 vs. ExPASy TrEMBL
Match: A0A6J1DXY1 (putative disease resistance protein RGA3 OS=Momordica charantia OX=3673 GN=LOC111025175 PE=4 SV=1)

HSP 1 Score: 235 bits (600), Expect = 8.36e-69
Identity = 116/167 (69.46%), Postives = 140/167 (83.83%), Query Frame = 0


Query: 41  LYGCVSEPFDINKILEAILQNLKGPSGACDNREVLLHELKKEMRDKRYFLVLDDVWNENS 100
           ++ CVSEPF +NKIL AIL  L+G S   DN+E LLHELKKEM  KRYFLVLDDVWNE+ 
Sbjct: 224 IWVCVSEPFLVNKILGAILGTLRGTSSGLDNKEALLHELKKEMHGKRYFLVLDDVWNESH 283

Query: 101 SLWDELKNCLMRMTEKSENSIVVTTRNVEVAKIIETLPSHHLSKLSIDQFWSLFQESANA 160
           SLWDELKNCLMR+TE+SENSI+VTTR+ EVAKI+ETLP+HHLSKLS DQ WSLF ESAN 
Sbjct: 284 SLWDELKNCLMRITERSENSILVTTRSAEVAKIMETLPNHHLSKLSDDQCWSLFLESANV 343

Query: 161 NGLPMTLSLEIVRERLIKKIGGTLLVAKVWGKTVQFQGNYDGWVTKI 207
           NGL +T SL++V+E L+KKIGG  LVA+V G+ V+F+G+Y+ WVT +
Sbjct: 344 NGLSITSSLKVVQE-LVKKIGGIPLVARVLGRAVKFEGDYETWVTTL 389

[Close]

BLAST of MC02g_new0189 vs. ExPASy TrEMBL
Match: A0A6J1D340 (disease resistance protein RGA2-like OS=Momordica charantia OX=3673 GN=LOC111017143 PE=4 SV=1)

HSP 1 Score: 228 bits (580), Expect = 2.27e-65
Identity = 113/168 (67.26%), Postives = 134/168 (79.76%), Query Frame = 0


Query: 41  LYGCVSEPFDINKILEAILQNLKG-PSGACDNREVLLHELKKEMRDKRYFLVLDDVWNEN 100
           ++ CVS+PF++NKIL  IL+ LK  PSG    +EVLL +L KEMR K+YFLVLDDVWNEN
Sbjct: 225 IWVCVSQPFNVNKILGTILETLKDTPSGLEYKKEVLLGQLYKEMRGKKYFLVLDDVWNEN 284

Query: 101 SSLWDELKNCLMRMTEKSENSIVVTTRNVEVAKIIETLPSHHLSKLSIDQFWSLFQESAN 160
             LWDELKNCLM++TE SEN I+VTTR+ EVAKI+ETLPSHHL KLS DQ WSLFQESAN
Sbjct: 285 RLLWDELKNCLMKITENSENGILVTTRSAEVAKIMETLPSHHLGKLSDDQCWSLFQESAN 344

Query: 161 ANGLPMTLSLEIVRERLIKKIGGTLLVAKVWGKTVQFQGNYDGWVTKI 207
           ANGL MT SL++VRE L+KKIGG  L+AKVWG   Q+ G+ D WV  +
Sbjct: 345 ANGLSMTSSLKVVREELVKKIGGLPLIAKVWGSAEQYLGDPDKWVINL 392

[Close]

BLAST of MC02g_new0189 vs. ExPASy TrEMBL
Match: A0A6J1FYJ5 (disease resistance protein RGA2-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111448744 PE=4 SV=1)

HSP 1 Score: 220 bits (561), Expect = 5.82e-63
Identity = 111/168 (66.07%), Postives = 134/168 (79.76%), Query Frame = 0


Query: 41  LYGCVSEPFDINKILEAILQNLKGPSGACDNREVLLHELKKEMRDKRYFLVLDDVWNENS 100
           ++ CVS PFDINKIL AILQN+KG  G   NRE+LL ELKKEM  KRYFLVLDDVWNE+S
Sbjct: 227 IWVCVSIPFDINKILGAILQNVKGHDGCYHNREILLRELKKEMYGKRYFLVLDDVWNEDS 286

Query: 101 SLWDELKNCLMRMTEKSENSIVVTTRNVEVAKIIE-TLPSHHLSKLSIDQFWSLFQESAN 160
            LW++LKN LM++T KS NSI+VTTRNVEV KI+E  LP+HH++KLS +Q WSLF ESAN
Sbjct: 287 LLWEQLKNFLMKITSKSGNSILVTTRNVEVCKIMEKALPTHHITKLSDEQCWSLFMESAN 346

Query: 161 ANGLPMTLSLEIVRERLIKKIGGTLLVAKVWGKTVQFQGNYDGWVTKI 207
           ANGLP T +LE VRE L+KK GG  LVA+V G  V+F+ +YD WV+ +
Sbjct: 347 ANGLPTTSNLEAVREELVKKFGGIPLVARVLGSAVKFEQDYDIWVSLL 394

[Close]

BLAST of MC02g_new0189 vs. ExPASy TrEMBL
Match: A0A6J1FWC6 (disease resistance protein RGA2-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111448744 PE=4 SV=1)

HSP 1 Score: 220 bits (561), Expect = 7.07e-63
Identity = 111/168 (66.07%), Postives = 134/168 (79.76%), Query Frame = 0


Query: 41  LYGCVSEPFDINKILEAILQNLKGPSGACDNREVLLHELKKEMRDKRYFLVLDDVWNENS 100
           ++ CVS PFDINKIL AILQN+KG  G   NRE+LL ELKKEM  KRYFLVLDDVWNE+S
Sbjct: 199 IWVCVSIPFDINKILGAILQNVKGHDGCYHNREILLRELKKEMYGKRYFLVLDDVWNEDS 258

Query: 101 SLWDELKNCLMRMTEKSENSIVVTTRNVEVAKIIE-TLPSHHLSKLSIDQFWSLFQESAN 160
            LW++LKN LM++T KS NSI+VTTRNVEV KI+E  LP+HH++KLS +Q WSLF ESAN
Sbjct: 259 LLWEQLKNFLMKITSKSGNSILVTTRNVEVCKIMEKALPTHHITKLSDEQCWSLFMESAN 318

Query: 161 ANGLPMTLSLEIVRERLIKKIGGTLLVAKVWGKTVQFQGNYDGWVTKI 207
           ANGLP T +LE VRE L+KK GG  LVA+V G  V+F+ +YD WV+ +
Sbjct: 319 ANGLPTTSNLEAVREELVKKFGGIPLVARVLGSAVKFEQDYDIWVSLL 366

[Close]

BLAST of MC02g_new0189 vs. TAIR 10
Match: AT3G14470.1 (NB-ARC domain-containing disease resistance protein )

HSP 1 Score: 82.8 bits (203), Expect = 3.6e-16
Identity = 56/168 (33.33%), Postives = 90/168 (53.57%), Query Frame = 0


Query: 40  KLYGCVSEPFDINKILEAILQNLKGPSGACDNREVLLHELKKEMRDK--RYFLVLDDVWN 99
           K++  VSE FD+ KI + + +++        + +VL  +LK+ +      + LVLDD+WN
Sbjct: 229 KVWAHVSEEFDVFKITKKVYESVTSRPCEFTDLDVLQVKLKERLTGTGLPFLLVLDDLWN 288

Query: 100 ENSSLWDELKNCLMRMTEKSENSIVVTTRNVEVAKIIETLPSHHLSKLSIDQFWSLFQES 159
           EN + WD L+   +   + S+  I+VTTR+  VA I+  +  H+L  LS    WSLF ++
Sbjct: 289 ENFADWDLLRQPFIHAAQGSQ--ILVTTRSQRVASIMCAVHVHNLQPLSDGDCWSLFMKT 348

Query: 160 ANANGLPMTLSLEI--VRERLIKKIGGTLLVAKVWGKTVQFQGNYDGW 204
              N  P  L+ EI  + ER++ K  G  L  K  G  ++F+G    W
Sbjct: 349 VFGNQEP-CLNREIGDLAERIVHKCRGLPLAVKTLGGVLRFEGKVIEW 393

[Close]

BLAST of MC02g_new0189 vs. TAIR 10
Match: AT3G50950.2 (HOPZ-ACTIVATED RESISTANCE 1 )

HSP 1 Score: 73.2 bits (178), Expect = 2.8e-13
Identity = 52/156 (33.33%), Postives = 83/156 (53.21%), Query Frame = 0


Query: 40  KLYGCVSEPFDINKILEAILQNLKGPSGACDNREVLLHELKKEMRDKRYFLVLDDVWNEN 99
           +++  VS+ F   +I+ +IL+NL G +   D+   LL ++++ +  KRY +V+DDVW++N
Sbjct: 215 RIWVSVSQTFTEEQIMRSILRNL-GDASVGDDIGTLLRKIQQYLLGKRYLIVMDDVWDKN 274

Query: 100 SSLWDELKNCLMRMTEKSENSIVVTTRNVEVAKIIETL--PSHHLSKLSIDQFWSLFQES 159
            S WD++   L R       S++VTTR+  VAK ++     +H    LS D  W LF   
Sbjct: 275 LSWWDKIYQGLPR---GQGGSVIVTTRSESVAKRVQARDDKTHRPELLSPDNSWLLFCNV 334

Query: 160 ANA--NGLPMTLSLEIVRERLIKKIGGTLLVAKVWG 192
           A A  +G      LE V + ++ K  G  L  K  G
Sbjct: 335 AFAANDGTCERPELEDVGKEIVTKCKGLPLTIKAVG 366

[Close]

BLAST of MC02g_new0189 vs. TAIR 10
Match: AT3G50950.1 (HOPZ-ACTIVATED RESISTANCE 1 )

HSP 1 Score: 73.2 bits (178), Expect = 2.8e-13
Identity = 52/156 (33.33%), Postives = 83/156 (53.21%), Query Frame = 0


Query: 40  KLYGCVSEPFDINKILEAILQNLKGPSGACDNREVLLHELKKEMRDKRYFLVLDDVWNEN 99
           +++  VS+ F   +I+ +IL+NL G +   D+   LL ++++ +  KRY +V+DDVW++N
Sbjct: 215 RIWVSVSQTFTEEQIMRSILRNL-GDASVGDDIGTLLRKIQQYLLGKRYLIVMDDVWDKN 274

Query: 100 SSLWDELKNCLMRMTEKSENSIVVTTRNVEVAKIIETL--PSHHLSKLSIDQFWSLFQES 159
            S WD++   L R       S++VTTR+  VAK ++     +H    LS D  W LF   
Sbjct: 275 LSWWDKIYQGLPR---GQGGSVIVTTRSESVAKRVQARDDKTHRPELLSPDNSWLLFCNV 334

Query: 160 ANA--NGLPMTLSLEIVRERLIKKIGGTLLVAKVWG 192
           A A  +G      LE V + ++ K  G  L  K  G
Sbjct: 335 AFAANDGTCERPELEDVGKEIVTKCKGLPLTIKAVG 366

[Close]

BLAST of MC02g_new0189 vs. TAIR 10
Match: AT3G14460.1 (LRR and NB-ARC domains-containing disease resistance protein )

HSP 1 Score: 64.7 bits (156), Expect = 1.0e-10
Identity = 42/168 (25.00%), Postives = 86/168 (51.19%), Query Frame = 0


Query: 39  IKLYGCVSEPFDINKILEAILQNLKGPSGACDNREVLLHELKKEMRDKRYFLVLDDVWNE 98
           +K++      F++  + +A+LQ++   +   ++   L  +LKK +  KR+ LVLDD W+E
Sbjct: 225 VKMWISAGINFNVFTVTKAVLQDITSSAVNTEDLPSLQIQLKKTLSGKRFLLVLDDFWSE 284

Query: 99  NSSLWDELKNCLMRMTEKSENS-IVVTTRNVEVAKIIETLPSHHLSKLSIDQFWSLFQES 158
           + S W+  +   +  T+  E S IV+TTR+  V+ + +    + +  ++ ++ W L    
Sbjct: 285 SDSEWESFQ---VAFTDAEEGSKIVLTTRSEIVSTVAKAEKIYQMKLMTNEECWELISRF 344

Query: 159 A--NANGLPMTLSLEIVRERLIKKIGGTLLVAKVWGKTVQFQGNYDGW 204
           A  N +   +   LE + +R+ ++  G  L A+     ++ + N D W
Sbjct: 345 AFGNISVGSINQELEGIGKRIAEQCKGLPLAARAIASHLRSKPNPDDW 389

[Close]

BLAST of MC02g_new0189 vs. TAIR 10
Match: AT3G46730.1 (NB-ARC domain-containing disease resistance protein )

HSP 1 Score: 55.8 bits (133), Expect = 4.7e-08
Identity = 42/154 (27.27%), Postives = 80/154 (51.95%), Query Frame = 0


Query: 45  VSEPFDINKILEAILQNLKGPSGACDNREVLLHELKKE--------MRDKRYFLVLDDVW 104
           VS+ +    IL  I+++L G   A +  ++ + E  +E        +  K Y +V+DDVW
Sbjct: 223 VSQEYKTRDILIRIIRSL-GIVSAEEMEKIKMFEEDEELEVYLYGLLEGKNYMVVVDDVW 282

Query: 105 NENSSLWDELKNCLMRMTEKSENSIVVTTRNVEVAKIIE-TLPSHHLSKLSIDQFWSLFQ 164
           + ++  W+ LK  L    +   + +++TTR   +A+ +E T+ +H L  L+ ++ W+LF+
Sbjct: 283 DPDA--WESLKRAL--PCDHRGSKVIITTRIRAIAEGVEGTVYAHKLRFLTFEESWTLFE 342

Query: 165 ESANANGLPMTLSLEIVRERLIKKIGGTLLVAKV 190
             A +N   +   L+   + ++KK GG  L   V
Sbjct: 343 RKAFSNIEKVDEDLQRTGKEMVKKCGGLPLAIVV 371

The following BLAST results are available for this feature:

BLAST of MC02g_new0189 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q7XA40	6.2e-21	34.55	Putative disease resistance protein RGA3 OS=Solanum bulbocastanum OX=147425 GN=R...	[more]
Q7XA42	4.9e-18	32.32	Putative disease resistance protein RGA1 OS=Solanum bulbocastanum OX=147425 GN=R...	[more]
Q7XBQ9	1.9e-17	33.94	Disease resistance protein RGA2 OS=Solanum bulbocastanum OX=147425 GN=RGA2 PE=1 ...	[more]
Q7XA39	2.7e-16	31.71	Putative disease resistance protein RGA4 OS=Solanum bulbocastanum OX=147425 GN=R...	[more]
Q9LRR4	5.0e-15	33.33	Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana OX=3702...	[more]

BLAST of MC02g_new0189 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022150842.1	5.58e-74	73.94	putative disease resistance protein RGA3 [Momordica charantia]	[more]
XP_038901403.1	3.29e-73	71.26	putative disease resistance protein RGA3 isoform X3 [Benincasa hispida]	[more]
XP_038901402.1	4.82e-72	71.26	putative disease resistance protein RGA3 isoform X2 [Benincasa hispida]	[more]
XP_038901401.1	6.99e-72	71.26	disease resistance protein RGA2-like isoform X1 [Benincasa hispida]	[more]
XP_022158717.1	1.73e-68	69.46	putative disease resistance protein RGA3 [Momordica charantia]	[more]

BLAST of MC02g_new0189 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DBV6	2.70e-74	73.94	putative disease resistance protein RGA3 OS=Momordica charantia OX=3673 GN=LOC11...	[more]
A0A6J1DXY1	8.36e-69	69.46	putative disease resistance protein RGA3 OS=Momordica charantia OX=3673 GN=LOC11...	[more]
A0A6J1D340	2.27e-65	67.26	disease resistance protein RGA2-like OS=Momordica charantia OX=3673 GN=LOC111017...	[more]
A0A6J1FYJ5	5.82e-63	66.07	disease resistance protein RGA2-like isoform X2 OS=Cucurbita moschata OX=3662 GN...	[more]
A0A6J1FWC6	7.07e-63	66.07	disease resistance protein RGA2-like isoform X1 OS=Cucurbita moschata OX=3662 GN...	[more]

BLAST of MC02g_new0189 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G14470.1	3.6e-16	33.33	NB-ARC domain-containing disease resistance protein	[more]
AT3G50950.2	2.8e-13	33.33	HOPZ-ACTIVATED RESISTANCE 1	[more]
AT3G50950.1	2.8e-13	33.33	HOPZ-ACTIVATED RESISTANCE 1	[more]
AT3G14460.1	1.0e-10	25.00	LRR and NB-ARC domains-containing disease resistance protein	[more]
AT3G46730.1	4.7e-08	27.27	NB-ARC domain-containing disease resistance protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002182	NB-ARC	PFAM	PF00931	NB-ARC	coord: 10..201 e-value: 4.7E-24 score: 84.9
IPR027417	P-loop containing nucleoside triphosphate hydrolase	GENE3D	3.40.50.300		coord: 32..137 e-value: 3.2E-14 score: 54.8
IPR027417	P-loop containing nucleoside triphosphate hydrolase	SUPERFAMILY	52540	P-loop containing nucleoside triphosphate hydrolases	coord: 41..204
None	No IPR available	PANTHER	PTHR23155:SF1139	NBS-LRR TYPE DISEASE RESISTANCE PROTEIN	coord: 40..194
IPR044974	Disease resistance protein, plants	PANTHER	PTHR23155	DISEASE RESISTANCE PROTEIN RP	coord: 40..194

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC02g_new0189.1	MC02g_new0189.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006952	defense response
molecular_function	GO:0043531	ADP binding