MC02g_new0178 (gene) Bitter gourd (Dali-11) v1

BLAST of MC02g_new0178 vs. ExPASy Swiss-Prot
Match: A0A290U7C4 (Disease resistance protein Roq1 OS=Nicotiana benthamiana OX=4100 GN=ROQ1 PE=1 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 1.5e-27
Identity = 86/256 (33.59%), Postives = 126/256 (49.22%), Query Frame = 0

Query: 4   VEVCLQTEIFSKIKNLRLLIIREAN-VIGSP-QYLSNKLKWLEWAGPCSIAPPSTFYPQK 63
           +E     E   K + LR+L+    N     P  YL N L WLEW    S + PS F P K
Sbjct: 542 LEYMYSAEALKKTRRLRILVKEYYNRGFDEPVAYLPNSLLWLEWRNYSSNSFPSNFEPSK 601

Query: 64  LVVLNIQHSSIKKFGIGLKG-----------------------FKNLRYLNFNGCETLTE 123
           LV L ++ SSI +   G K                          NL  L  + C+ L E
Sbjct: 602 LVYLTMKGSSIIELWNGAKRLAFLTTLDLSYCHKLIQTPDFRMITNLERLILSSCDALVE 661

Query: 124 I-CDISVMPNLVILDLQQCSNLEFFSSGLKSKSLEVLNLGHCYKLIKFPDVVEEMECLNI 183
           +   +  + NL++L++  C +LE   + ++S+ LEVL+L +C+ L  FP+V   M  L  
Sbjct: 662 VHPSVGFLKNLILLNMDHCISLERLPAIIQSECLEVLDLNYCFNLKMFPEVERNMTHLKK 721

Query: 184 IDLRASGITELPTSIENITGLRFLNLDFCEHLVLLPSSIYRLKNLDYIGLNACFRLLKFP 233
           +DL ++GI ELP SIE+++ L  L +  C  LV LPSSI+R +NL    ++ C +L   P
Sbjct: 722 LDLTSTGIRELPASIEHLSSLENLQMHSCNQLVSLPSSIWRFRNLK---ISECEKLGSLP 781

BLAST of MC02g_new0178 vs. ExPASy Swiss-Prot
Match: Q9SZ67 (Probable WRKY transcription factor 19 OS=Arabidopsis thaliana OX=3702 GN=WRKY19 PE=1 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 1.2e-24
Identity = 85/259 (32.82%), Postives = 125/259 (48.26%), Query Frame = 0

Query: 1    MPNVEVCLQTEIFSKIKNLRLLII-----REANVIGSPQ---YLSNKLKWLEWAGPCSIA 60
            M N++      +F K+ NLRLL +      E + +  PQ   YL +KL+ L W      +
Sbjct: 1160 MLNLKFDANPNVFEKMCNLRLLKLYCSKAEEKHGVSFPQGLEYLPSKLRLLHWEYYPLSS 1219

Query: 61   PPSTFYPQKLVVLNIQHSSIKKFGIG-------------------------------LKG 120
             P +F P+ LV LN+  S  KK   G                               L  
Sbjct: 1220 LPKSFNPENLVELNLPSSCAKKLWKGKKARFCTTNSSLEKLKKMRLSYSDQLTKIPRLSS 1279

Query: 121  FKNLRYLNFNGCETLTEIC-DISVMPNLVILDLQQCSNLEFFSSGLKSKSLEVLNLGHCY 180
              NL +++  GC +L  +   IS +  LV L+L+ CS LE   S +  +SLEVLNL  C 
Sbjct: 1280 ATNLEHIDLEGCNSLLSLSQSISYLKKLVFLNLKGCSKLENIPSMVDLESLEVLNLSGCS 1339

Query: 181  KLIKFPDVVEEMECLNIIDLRASG--ITELPTSIENITGLRFLNLDFCEHLVLLPSSIYR 218
            KL  FP++       N+ +L   G  I E+P+SI+N+  L  L+L+   HL  LP+SIY+
Sbjct: 1340 KLGNFPEISP-----NVKELYMGGTMIQEIPSSIKNLVLLEKLDLENSRHLKNLPTSIYK 1399

BLAST of MC02g_new0178 vs. ExPASy Swiss-Prot
Match: F4J339 (Probable disease resistance protein RPP1 OS=Arabidopsis thaliana OX=3702 GN=RPP1 PE=1 SV=1)

HSP 1 Score: 110.5 bits (275), Expect = 3.9e-23
Identity = 70/199 (35.18%), Postives = 108/199 (54.27%), Query Frame = 0

Query: 35  YLSNKLKWLEWAGPCSIAPPSTFYPQKLVVLNIQHSSIKKFGIGLKGFKNLRYLNFNGCE 94
           Y S K++ L W G  S+  PSTF P+ LV L+++ S+++K   G K  +NL++++ +   
Sbjct: 666 YHSPKIRSLNWYGYESLCLPSTFNPEFLVELDMRSSNLRKLWEGTKQLRNLKWMDLSYSS 725

Query: 95  TLTEICDISVMPNLVILDLQQCSNL-EFFSSGLKSKSLEVLNLGHCYKLIKFPDVVEEME 154
            L E+ ++S   NL  L L+ CS+L E  SS  K  SL++L+L +C  L K P +    +
Sbjct: 726 YLKELPNLSTATNLEELKLRNCSSLVELPSSIEKLTSLQILDLENCSSLEKLPAIENATK 785

Query: 155 CLNIIDLRASGITELPTSIENITGLRFLNLDFCEHLVLLPSSIYRLKNLDYIGLNACFRL 214
              +     S + ELP SI   T L+ LN+  C  LV LPSSI  + +L+   L+ C  L
Sbjct: 786 LRELKLQNCSSLIELPLSIGTATNLKQLNISGCSSLVKLPSSIGDITDLEVFDLSNCSSL 845

Query: 215 LKFPQRLNSAVVSSNLLKL 233
           +  P  + +     NL KL
Sbjct: 846 VTLPSSIGNL---QNLCKL 861

BLAST of MC02g_new0178 vs. ExPASy Swiss-Prot
Match: F4JT80 (Disease resistance protein RPP2B OS=Arabidopsis thaliana OX=3702 GN=RPP2B PE=1 SV=2)

HSP 1 Score: 102.1 bits (253), Expect = 1.4e-20
Identity = 66/208 (31.73%), Postives = 100/208 (48.08%), Query Frame = 0

Query: 33  PQYLSNKLKWLEWAGPCSIAPPSTFYPQKLVVLNIQHSSIKKFG---------------- 92
           P +  ++L +L W G      PS F P++LV L++++S IK+                  
Sbjct: 581 PDHFPDELVYLHWQGYPYDCLPSDFDPKELVDLSLRYSHIKQLWEDEKNTESLRWVDLGQ 640

Query: 93  -------IGLKGFKNLRYLNFNGCETLTEICDISVMPNLVILDLQQCSNLEFFSSGLKSK 152
                   GL   KNL  L+  GC +L  +  +  M  L+ L+L+ C++LE    G K K
Sbjct: 641 SKDLLNLSGLSRAKNLERLDLEGCTSLDLLGSVKQMNELIYLNLRDCTSLESLPKGFKIK 700

Query: 153 SLEVLNLGHCYKLIKFPDVVEEMECLNIIDLRASGITELPTSIENITGLRFLNLDFCEHL 212
           SL+ L L  C KL  F  + E +E L+   L  + I  +   IE++  L  LNL  CE L
Sbjct: 701 SLKTLILSGCLKLKDFHIISESIESLH---LEGTAIERVVEHIESLHSLILLNLKNCEKL 760

Query: 213 VLLPSSIYRLKNLDYIGLNACFRLLKFP 218
             LP+ +Y+LK+L  + L+ C  L   P
Sbjct: 761 KYLPNDLYKLKSLQELVLSGCSALESLP 785

BLAST of MC02g_new0178 vs. ExPASy Swiss-Prot
Match: Q40392 (TMV resistance protein N OS=Nicotiana glutinosa OX=35889 GN=N PE=1 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 1.1e-17
Identity = 73/232 (31.47%), Postives = 113/232 (48.71%), Query Frame = 0

Query: 16  IKNLRLLIIREANVIGSPQYLSNKLKWLEWAGPCSIAPPSTFYPQKLVVLNIQHSSIKKF 75
           +K LR+  +  ++   +  YL N L+          + PSTF  + LV L ++H+S++  
Sbjct: 552 MKRLRVFNMGRSSTHYAIDYLPNNLRCFVCTNYPWESFPSTFELKMLVHLQLRHNSLRHL 611

Query: 76  GIGLKGFKNLRYLNFNGCETLTEICDISVMPNLVILDLQQCSNLE--FFSSGLKSK---- 135
               K   +LR ++ +  + LT   D + MPNL  ++L QCSNLE    S G  SK    
Sbjct: 612 WTETKHLPSLRRIDLSWSKRLTRTPDFTGMPNLEYVNLYQCSNLEEVHHSLGCCSKVIGL 671

Query: 136 -----------------SLEVLNLGHCYKLIKFPDVVEEMECLNIIDLRASGITELPTSI 195
                            SLE L L  C  L K P++   M+    I ++ SGI ELP+SI
Sbjct: 672 YLNDCKSLKRFPCVNVESLEYLGLRSCDSLEKLPEIYGRMKPEIQIHMQGSGIRELPSSI 731

Query: 196 ----ENITGLRFLNLDFCEHLVLLPSSIYRLKNLDYIGLNACFRLLKFPQRL 221
                ++T L   N+   ++LV LPSSI RLK+L  + ++ C +L   P+ +
Sbjct: 732 FQYKTHVTKLLLWNM---KNLVALPSSICRLKSLVSLSVSGCSKLESLPEEI 780

BLAST of MC02g_new0178 vs. NCBI nr
Match: XP_022148563.1 (TMV resistance protein N-like [Momordica charantia])

HSP 1 Score: 353 bits (906), Expect = 5.07e-111
Identity = 197/312 (63.14%), Postives = 221/312 (70.83%), Query Frame = 0

Query: 1   MPNVEVCLQTEIFSKIKNLRLLIIREANVIGSPQYLSNKLKWLEWAGPCSIAPPSTFYPQ 60
           MPNVEV LQTE F K+K LRLLIIREANVIG+PQYLSN+L+WLEW  P S+ P STF   
Sbjct: 535 MPNVEVSLQTETFLKMKKLRLLIIREANVIGTPQYLSNELRWLEWPAPSSMVP-STFRAN 594

Query: 61  KLVVLNIQHSSIKKFGIGLKGFKNLRYLNFNGCETLTEICDISVMPNLVILDLQQCSNLE 120
           KLVVLN+Q  SIKKF IGLKGF+NLRYLNFNGC+ LTEICDISVMPNLV LDLQ+CSNLE
Sbjct: 595 KLVVLNLQRCSIKKFEIGLKGFENLRYLNFNGCKMLTEICDISVMPNLVSLDLQRCSNLE 654

Query: 121 FFSSGLKSKSLEVLNLGHCYKLIKFPDVVEEMECLNIIDLRASGITELPTSIENITGLRF 180
           FFSSGLKSK L+ LNL  CYKL KFPD++EEMECL+ + LRASGITELP SIEN+TGL  
Sbjct: 655 FFSSGLKSKFLKDLNLDCCYKLKKFPDILEEMECLDTLSLRASGITELPASIENLTGLID 714

Query: 181 LNLDFCEHLVLLPSSIYRLKNLDYIGLNACFRLLKFPQRLNSAVVSS------------- 240
           LNLD CEHLV LPSSIYRLKN+ +I L+ CFRL  FPQ LNS VVSS             
Sbjct: 715 LNLDSCEHLVRLPSSIYRLKNVRFIVLDGCFRLETFPQPLNSVVVSSSSEGKRIQDSMTC 774

Query: 241 -----------------NLLKLDWIMPFNHFYSSVILTF---KERKLPMGMSKFDGHNSL 279
                            N LKLD+IM FN F S   L     K R+LP GMSKF+G   +
Sbjct: 775 LDNRYHSTLAEFRLMQSNFLKLDFIMAFNCFNSLRSLHLNGSKIRRLPRGMSKFEGLELI 834

BLAST of MC02g_new0178 vs. NCBI nr
Match: XP_038902439.1 (disease resistance protein RUN1-like [Benincasa hispida])

HSP 1 Score: 226 bits (577), Expect = 1.86e-63
Identity = 127/252 (50.40%), Postives = 164/252 (65.08%), Query Frame = 0

Query: 2   PNVEVC-LQTEIFSKIKNLRLLIIRE---ANVIGSPQYLSNKLKWLEWAGPCSIAPPSTF 61
           P  EV  L  + FS++K LRLLIIR      VIG+P+ LS +L+W E     S   PSTF
Sbjct: 541 PKAEVVWLNAKAFSRMKKLRLLIIRNNVNVIVIGTPRCLSKELRWFECRRSSSSLVPSTF 600

Query: 62  YPQKLVVLNIQHSSIKKFGIGLKGFKNLRYLNFNGCETLTEICDISVMPNLVILDLQQCS 121
           +PQKLVV  I H  IK+    LK F+++R L+   CETLTE+ DIS+MPNL+ L++  C 
Sbjct: 601 HPQKLVVFGISHYPIKQLVANLKNFESIRQLSLENCETLTEVGDISLMPNLIRLEIINCF 660

Query: 122 NLEFFSS-GLKSKSLEVLNLGHCYKLIKFPDVVEEMECLNIIDLRASGITELPTSIENIT 181
           NLE FS+ GLKSK+L+ L+L  CYKL KFPD+VEEMEC+ I+ L  +G+TELPTSIE ++
Sbjct: 661 NLECFSNVGLKSKTLQRLHLKSCYKLKKFPDIVEEMECIEILSLEGTGVTELPTSIEKLS 720

Query: 182 GLRFLNLDFCEHLVLLPSSIYRLKNLDYIGLNACFRLLKFPQRLNSAVVSSNLLKLDWIM 241
           GLR L L  CE LV LPS+IY LK L+ + L+ C +L +FPQ  NS       L L  + 
Sbjct: 721 GLRHLELGLCEDLVHLPSTIYELKMLNALKLSKCHKLQEFPQPSNSITNVKKFLPLRLLN 780

Query: 242 PFNHFYSSVILT 248
             N   +SV  T
Sbjct: 781 QLNPLSTSVTYT 792

BLAST of MC02g_new0178 vs. NCBI nr
Match: XP_016902380.1 (PREDICTED: TMV resistance protein N-like [Cucumis melo])

HSP 1 Score: 210 bits (535), Expect = 1.68e-57
Identity = 124/249 (49.80%), Postives = 156/249 (62.65%), Query Frame = 0

Query: 1   MPNVE--VCLQTEIFSKIKNLRLLIIRE-ANVIGSPQYLSNKLKWLEWAGPCSIAPPSTF 60
           +P  E  V L  + FSK+  LRLLIIR   NVIG+P+  SN L+W E     S   P TF
Sbjct: 541 LPKAEEVVWLDAKSFSKMTKLRLLIIRNNVNVIGTPRCFSNYLRWFECHRSSSSLVPYTF 600

Query: 61  YPQKLVVLNIQHSSIKKFGIGLKGFKNLRYLNFNGCETLTEICDISVMPNLVILDLQQCS 120
            P+ LVVL I H  I      LK F+++R L+   CE LTEI DIS+MPNL+ L++  C 
Sbjct: 601 NPKNLVVLVISHYPIMHLVANLKCFESIRQLSLENCEMLTEIGDISLMPNLIKLEIINCF 660

Query: 121 NLEFFSS-GLKSKSLEVLNLGHCYKLIKFPDVVEEMECLNIIDLRASGITELPTSIENIT 180
           NLE FSS GLKSK+L+ L+L  CYKL KFPD+VEEMECL I+ L   G+TELP SIE ++
Sbjct: 661 NLECFSSTGLKSKTLQCLHLKSCYKLKKFPDIVEEMECLQILSLEEIGVTELPKSIEKLS 720

Query: 181 GLRFLNLDFCEHLVLLPSSIYRLKNLDYIGLNACFRLLKFPQRLNSAVVSSNLLKLDWIM 240
           GLR L L  CE L+ LPS IY LK L+ + L+ C +L +FPQ  NS+      L L  + 
Sbjct: 721 GLRHLELSLCEDLLQLPSIIYDLKMLNSLKLSKCLKLQEFPQPSNSSTNVKKFLPLRLLN 780

Query: 241 PFNHFYSSV 245
             N   +SV
Sbjct: 781 QKNLLSTSV 789

BLAST of MC02g_new0178 vs. NCBI nr
Match: TYK06530.1 (TMV resistance protein N-like [Cucumis melo var. makuwa])

HSP 1 Score: 210 bits (535), Expect = 1.40e-56
Identity = 121/239 (50.63%), Postives = 153/239 (64.02%), Query Frame = 0

Query: 1   MPNVE--VCLQTEIFSKIKNLRLLIIRE-ANVIGSPQYLSNKLKWLEWAGPCSIAPPSTF 60
           +P  E  V L  + FSK+  LRLLIIR   NVIG+P+  SN L+W E     S   P TF
Sbjct: 541 LPKAEEVVWLDAKSFSKMTKLRLLIIRNNVNVIGTPRCFSNYLRWFECHRSSSSLVPYTF 600

Query: 61  YPQKLVVLNIQHSSIKKFGIGLKGFKNLRYLNFNGCETLTEICDISVMPNLVILDLQQCS 120
            P+ LVVL I H  I      LK F+++R L+   CE LTEI DIS+MPNL+ L++  C 
Sbjct: 601 NPKNLVVLVISHYPIMHLVANLKCFESIRQLSLENCEMLTEIGDISLMPNLIKLEIINCF 660

Query: 121 NLEFFSS-GLKSKSLEVLNLGHCYKLIKFPDVVEEMECLNIIDLRASGITELPTSIENIT 180
           NLE FSS GLKSK+L+ L+L  CYKL KFPD+VEEMECL I+ L   G+TELP SIE ++
Sbjct: 661 NLECFSSTGLKSKTLQCLHLKSCYKLKKFPDIVEEMECLQILSLEEIGVTELPKSIEKLS 720

Query: 181 GLRFLNLDFCEHLVLLPSSIYRLKNLDYIGLNACFRLLKFPQRLNSAVVSSNLLKLDWI 235
           GLR L L  CE L+ LPS IY LK L+ + L+ C +L +FPQ  NS+      L L ++
Sbjct: 721 GLRHLELSLCEDLLQLPSIIYDLKMLNSLKLSKCLKLQEFPQPSNSSTNVKKFLPLRFL 779

BLAST of MC02g_new0178 vs. NCBI nr
Match: KAF9826339.1 (hypothetical protein H0E87_028010 [Populus deltoides])

HSP 1 Score: 191 bits (484), Expect = 7.92e-50
Identity = 102/240 (42.50%), Postives = 142/240 (59.17%), Query Frame = 0

Query: 5   EVCLQTEIFSKIKNLRLLIIREANVIGSPQYLSNKLKWLEWAGPCSIAPPSTFYPQKLVV 64
           EV L  + F K+K LR+LI+  A VIG P+ L N L+WLEW G    + PSTF+ +KLVV
Sbjct: 540 EVHLSAKAFEKMKRLRILIVHNAQVIGGPENLPNALRWLEWPGCPLKSMPSTFHARKLVV 599

Query: 65  LNIQHSSIKKFGIGLKGFKNLRYLNFNGCETLTEICDISVMPNL---------------- 124
           LN+ HS IK+ G G K +  L+Y++F  CE LTE  D S +PNL                
Sbjct: 600 LNMHHSCIKQLGEGFKNYTTLKYIDFRDCEFLTETPDFSTIPNLERLNLEGCTSLVKIHN 659

Query: 125 --------VILDLQQCSNLEFFSSGLKSKSLEVLNLGHCYKLIKFPDVVEEMECLNIIDL 184
                   V L ++ CSNLE  SS  + +SL+ L L  C KL KFPD+ ++M  +  + L
Sbjct: 660 SVGYLAKLVFLSVEFCSNLESISSHFRLRSLQTLLLTGCSKLEKFPDIEDKMASIERVSL 719

Query: 185 RASGITELPTSIENITGLRFLNLDFCEHLVLLPSSIYRLKNLDYIGLNACFRLLKFPQRL 220
            ++ I ELP SIEN+ GL+ + L FC +L  +PSSIY+L++L  + L+ C  L KFP+ +
Sbjct: 720 NSTAIKELPLSIENLVGLKVMTLSFCRNLSRIPSSIYKLQHLKRLLLDGCSELTKFPENM 779

BLAST of MC02g_new0178 vs. ExPASy TrEMBL
Match: A0A6J1D4F5 (TMV resistance protein N-like OS=Momordica charantia OX=3673 GN=LOC111017198 PE=4 SV=1)

HSP 1 Score: 353 bits (906), Expect = 2.46e-111
Identity = 197/312 (63.14%), Postives = 221/312 (70.83%), Query Frame = 0

Query: 1   MPNVEVCLQTEIFSKIKNLRLLIIREANVIGSPQYLSNKLKWLEWAGPCSIAPPSTFYPQ 60
           MPNVEV LQTE F K+K LRLLIIREANVIG+PQYLSN+L+WLEW  P S+ P STF   
Sbjct: 535 MPNVEVSLQTETFLKMKKLRLLIIREANVIGTPQYLSNELRWLEWPAPSSMVP-STFRAN 594

Query: 61  KLVVLNIQHSSIKKFGIGLKGFKNLRYLNFNGCETLTEICDISVMPNLVILDLQQCSNLE 120
           KLVVLN+Q  SIKKF IGLKGF+NLRYLNFNGC+ LTEICDISVMPNLV LDLQ+CSNLE
Sbjct: 595 KLVVLNLQRCSIKKFEIGLKGFENLRYLNFNGCKMLTEICDISVMPNLVSLDLQRCSNLE 654

Query: 121 FFSSGLKSKSLEVLNLGHCYKLIKFPDVVEEMECLNIIDLRASGITELPTSIENITGLRF 180
           FFSSGLKSK L+ LNL  CYKL KFPD++EEMECL+ + LRASGITELP SIEN+TGL  
Sbjct: 655 FFSSGLKSKFLKDLNLDCCYKLKKFPDILEEMECLDTLSLRASGITELPASIENLTGLID 714

Query: 181 LNLDFCEHLVLLPSSIYRLKNLDYIGLNACFRLLKFPQRLNSAVVSS------------- 240
           LNLD CEHLV LPSSIYRLKN+ +I L+ CFRL  FPQ LNS VVSS             
Sbjct: 715 LNLDSCEHLVRLPSSIYRLKNVRFIVLDGCFRLETFPQPLNSVVVSSSSEGKRIQDSMTC 774

Query: 241 -----------------NLLKLDWIMPFNHFYSSVILTF---KERKLPMGMSKFDGHNSL 279
                            N LKLD+IM FN F S   L     K R+LP GMSKF+G   +
Sbjct: 775 LDNRYHSTLAEFRLMQSNFLKLDFIMAFNCFNSLRSLHLNGSKIRRLPRGMSKFEGLELI 834

BLAST of MC02g_new0178 vs. ExPASy TrEMBL
Match: A0A1S4E327 (TMV resistance protein N-like OS=Cucumis melo OX=3656 GN=LOC103498360 PE=4 SV=1)

HSP 1 Score: 210 bits (535), Expect = 8.14e-58
Identity = 124/249 (49.80%), Postives = 156/249 (62.65%), Query Frame = 0

Query: 1   MPNVE--VCLQTEIFSKIKNLRLLIIRE-ANVIGSPQYLSNKLKWLEWAGPCSIAPPSTF 60
           +P  E  V L  + FSK+  LRLLIIR   NVIG+P+  SN L+W E     S   P TF
Sbjct: 541 LPKAEEVVWLDAKSFSKMTKLRLLIIRNNVNVIGTPRCFSNYLRWFECHRSSSSLVPYTF 600

Query: 61  YPQKLVVLNIQHSSIKKFGIGLKGFKNLRYLNFNGCETLTEICDISVMPNLVILDLQQCS 120
            P+ LVVL I H  I      LK F+++R L+   CE LTEI DIS+MPNL+ L++  C 
Sbjct: 601 NPKNLVVLVISHYPIMHLVANLKCFESIRQLSLENCEMLTEIGDISLMPNLIKLEIINCF 660

Query: 121 NLEFFSS-GLKSKSLEVLNLGHCYKLIKFPDVVEEMECLNIIDLRASGITELPTSIENIT 180
           NLE FSS GLKSK+L+ L+L  CYKL KFPD+VEEMECL I+ L   G+TELP SIE ++
Sbjct: 661 NLECFSSTGLKSKTLQCLHLKSCYKLKKFPDIVEEMECLQILSLEEIGVTELPKSIEKLS 720

Query: 181 GLRFLNLDFCEHLVLLPSSIYRLKNLDYIGLNACFRLLKFPQRLNSAVVSSNLLKLDWIM 240
           GLR L L  CE L+ LPS IY LK L+ + L+ C +L +FPQ  NS+      L L  + 
Sbjct: 721 GLRHLELSLCEDLLQLPSIIYDLKMLNSLKLSKCLKLQEFPQPSNSSTNVKKFLPLRLLN 780

Query: 241 PFNHFYSSV 245
             N   +SV
Sbjct: 781 QKNLLSTSV 789

BLAST of MC02g_new0178 vs. ExPASy TrEMBL
Match: A0A5D3C5B5 (TMV resistance protein N-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold70G00650 PE=4 SV=1)

HSP 1 Score: 210 bits (535), Expect = 6.76e-57
Identity = 121/239 (50.63%), Postives = 153/239 (64.02%), Query Frame = 0

Query: 1   MPNVE--VCLQTEIFSKIKNLRLLIIRE-ANVIGSPQYLSNKLKWLEWAGPCSIAPPSTF 60
           +P  E  V L  + FSK+  LRLLIIR   NVIG+P+  SN L+W E     S   P TF
Sbjct: 541 LPKAEEVVWLDAKSFSKMTKLRLLIIRNNVNVIGTPRCFSNYLRWFECHRSSSSLVPYTF 600

Query: 61  YPQKLVVLNIQHSSIKKFGIGLKGFKNLRYLNFNGCETLTEICDISVMPNLVILDLQQCS 120
            P+ LVVL I H  I      LK F+++R L+   CE LTEI DIS+MPNL+ L++  C 
Sbjct: 601 NPKNLVVLVISHYPIMHLVANLKCFESIRQLSLENCEMLTEIGDISLMPNLIKLEIINCF 660

Query: 121 NLEFFSS-GLKSKSLEVLNLGHCYKLIKFPDVVEEMECLNIIDLRASGITELPTSIENIT 180
           NLE FSS GLKSK+L+ L+L  CYKL KFPD+VEEMECL I+ L   G+TELP SIE ++
Sbjct: 661 NLECFSSTGLKSKTLQCLHLKSCYKLKKFPDIVEEMECLQILSLEEIGVTELPKSIEKLS 720

Query: 181 GLRFLNLDFCEHLVLLPSSIYRLKNLDYIGLNACFRLLKFPQRLNSAVVSSNLLKLDWI 235
           GLR L L  CE L+ LPS IY LK L+ + L+ C +L +FPQ  NS+      L L ++
Sbjct: 721 GLRHLELSLCEDLLQLPSIIYDLKMLNSLKLSKCLKLQEFPQPSNSSTNVKKFLPLRFL 779

BLAST of MC02g_new0178 vs. ExPASy TrEMBL
Match: A0A2K1WMI4 (TIR domain-containing protein OS=Populus trichocarpa OX=3694 GN=POPTR_019G002500 PE=4 SV=1)

HSP 1 Score: 191 bits (484), Expect = 3.84e-50
Identity = 101/240 (42.08%), Postives = 142/240 (59.17%), Query Frame = 0

Query: 5   EVCLQTEIFSKIKNLRLLIIREANVIGSPQYLSNKLKWLEWAGPCSIAPPSTFYPQKLVV 64
           EV L  + F K+K LR+LI+  A VIG P+ L N L+WLEW G    + PSTF+ +KLVV
Sbjct: 540 EVHLSAKAFKKMKRLRILIVHNAQVIGGPENLPNALRWLEWPGCPLESMPSTFHARKLVV 599

Query: 65  LNIQHSSIKKFGIGLKGFKNLRYLNFNGCETLTEICDISVMPNL---------------- 124
           LN+ HS IK+ G G K +  L+Y++F  CE LTE  D S +PNL                
Sbjct: 600 LNMHHSCIKQLGEGFKNYTTLKYIDFRDCEFLTETPDFSTIPNLERLNLEGCTSLVKIHN 659

Query: 125 --------VILDLQQCSNLEFFSSGLKSKSLEVLNLGHCYKLIKFPDVVEEMECLNIIDL 184
                   V L ++ CSNL+  SS  + +SL+ L L  C KL KFPD+ ++M C+  + L
Sbjct: 660 SVGYLAKLVFLSVEFCSNLKSISSHFRLRSLQTLLLTGCSKLEKFPDIEDKMACIERVSL 719

Query: 185 RASGITELPTSIENITGLRFLNLDFCEHLVLLPSSIYRLKNLDYIGLNACFRLLKFPQRL 220
            ++ I ELP SIEN+ GL+ + L FC +L  +PSSIY+L++L  + L+ C  L  FP+ +
Sbjct: 720 NSTAIKELPLSIENLVGLKVMTLSFCRNLSRIPSSIYKLQHLKRLLLDGCSELTTFPENM 779

BLAST of MC02g_new0178 vs. ExPASy TrEMBL
Match: A0A5N5J2H0 (Uncharacterized protein OS=Salix brachista OX=2182728 GN=DKX38_028823 PE=4 SV=1)

HSP 1 Score: 175 bits (444), Expect = 2.80e-45
Identity = 106/247 (42.91%), Postives = 147/247 (59.51%), Query Frame = 0

Query: 5   EVCLQTEIFSKIKNLRLLIIREANVIGSPQYLSNKLKWLEWAGPCSIAPPSTFYPQKLVV 64
           E+ L  + F K+K LR+LII  A +  +P YL N+L+WLEW G   ++ PSTF+ +KLVV
Sbjct: 39  EIHLSAKAFKKMKRLRILIIHNALLAVAPVYLPNELRWLEWPGCPLLSLPSTFHARKLVV 98

Query: 65  LNIQHSSIKKFGIGLKGFKNLRYLNFNGCETLTEICDISVMPNLVILDLQQCSNL----- 124
           LN+QHS I  F  G K +  L+++NF+GCE L E  D S + NL  L+L+ C+NL     
Sbjct: 99  LNLQHSFIGHFE-GFKNYSYLKFMNFSGCEFLRETPDFSTIRNLERLNLEGCANLVEVHQ 158

Query: 125 --------EF----FSSGLKS-------KSLEVLNLGHCYKLIKFPDVVEEMECLNIIDL 184
                   EF    F S L++       KSL  L L  C KL  FPDV+E+M CL  + L
Sbjct: 159 SVGYLDKLEFLNVEFCSNLRTLPRIIKLKSLNTLLLSGCQKLEAFPDVIEKMPCLEKLCL 218

Query: 185 RASGITELPTSIENITGLRFLNLDFCEHLVLLPSSIYRLKNLDYIGLNACFRLLKFPQRL 227
             + I  LP+SI+NI+GL+ L L FC +L  LP+SIY+L  L+ + L  C +L KFP   
Sbjct: 219 SGTAIKVLPSSIKNISGLKVLTLAFCRNLADLPNSIYKLTLLECLLLEGCSKLDKFPLNG 278

BLAST of MC02g_new0178 vs. TAIR 10
Match: AT4G12020.1 (protein kinase family protein )

HSP 1 Score: 115.5 bits (288), Expect = 8.5e-26
Identity = 85/259 (32.82%), Postives = 125/259 (48.26%), Query Frame = 0

Query: 1    MPNVEVCLQTEIFSKIKNLRLLII-----REANVIGSPQ---YLSNKLKWLEWAGPCSIA 60
            M N++      +F K+ NLRLL +      E + +  PQ   YL +KL+ L W      +
Sbjct: 1160 MLNLKFDANPNVFEKMCNLRLLKLYCSKAEEKHGVSFPQGLEYLPSKLRLLHWEYYPLSS 1219

Query: 61   PPSTFYPQKLVVLNIQHSSIKKFGIG-------------------------------LKG 120
             P +F P+ LV LN+  S  KK   G                               L  
Sbjct: 1220 LPKSFNPENLVELNLPSSCAKKLWKGKKARFCTTNSSLEKLKKMRLSYSDQLTKIPRLSS 1279

Query: 121  FKNLRYLNFNGCETLTEIC-DISVMPNLVILDLQQCSNLEFFSSGLKSKSLEVLNLGHCY 180
              NL +++  GC +L  +   IS +  LV L+L+ CS LE   S +  +SLEVLNL  C 
Sbjct: 1280 ATNLEHIDLEGCNSLLSLSQSISYLKKLVFLNLKGCSKLENIPSMVDLESLEVLNLSGCS 1339

Query: 181  KLIKFPDVVEEMECLNIIDLRASG--ITELPTSIENITGLRFLNLDFCEHLVLLPSSIYR 218
            KL  FP++       N+ +L   G  I E+P+SI+N+  L  L+L+   HL  LP+SIY+
Sbjct: 1340 KLGNFPEISP-----NVKELYMGGTMIQEIPSSIKNLVLLEKLDLENSRHLKNLPTSIYK 1399

BLAST of MC02g_new0178 vs. TAIR 10
Match: AT4G12020.2 (protein kinase family protein )

HSP 1 Score: 115.5 bits (288), Expect = 8.5e-26
Identity = 85/259 (32.82%), Postives = 125/259 (48.26%), Query Frame = 0

Query: 1    MPNVEVCLQTEIFSKIKNLRLLII-----REANVIGSPQ---YLSNKLKWLEWAGPCSIA 60
            M N++      +F K+ NLRLL +      E + +  PQ   YL +KL+ L W      +
Sbjct: 1160 MLNLKFDANPNVFEKMCNLRLLKLYCSKAEEKHGVSFPQGLEYLPSKLRLLHWEYYPLSS 1219

Query: 61   PPSTFYPQKLVVLNIQHSSIKKFGIG-------------------------------LKG 120
             P +F P+ LV LN+  S  KK   G                               L  
Sbjct: 1220 LPKSFNPENLVELNLPSSCAKKLWKGKKARFCTTNSSLEKLKKMRLSYSDQLTKIPRLSS 1279

Query: 121  FKNLRYLNFNGCETLTEIC-DISVMPNLVILDLQQCSNLEFFSSGLKSKSLEVLNLGHCY 180
              NL +++  GC +L  +   IS +  LV L+L+ CS LE   S +  +SLEVLNL  C 
Sbjct: 1280 ATNLEHIDLEGCNSLLSLSQSISYLKKLVFLNLKGCSKLENIPSMVDLESLEVLNLSGCS 1339

Query: 181  KLIKFPDVVEEMECLNIIDLRASG--ITELPTSIENITGLRFLNLDFCEHLVLLPSSIYR 218
            KL  FP++       N+ +L   G  I E+P+SI+N+  L  L+L+   HL  LP+SIY+
Sbjct: 1340 KLGNFPEISP-----NVKELYMGGTMIQEIPSSIKNLVLLEKLDLENSRHLKNLPTSIYK 1399

BLAST of MC02g_new0178 vs. TAIR 10
Match: AT4G12020.3 (protein kinase family protein )

HSP 1 Score: 115.5 bits (288), Expect = 8.5e-26
Identity = 85/259 (32.82%), Postives = 125/259 (48.26%), Query Frame = 0

Query: 1    MPNVEVCLQTEIFSKIKNLRLLII-----REANVIGSPQ---YLSNKLKWLEWAGPCSIA 60
            M N++      +F K+ NLRLL +      E + +  PQ   YL +KL+ L W      +
Sbjct: 1160 MLNLKFDANPNVFEKMCNLRLLKLYCSKAEEKHGVSFPQGLEYLPSKLRLLHWEYYPLSS 1219

Query: 61   PPSTFYPQKLVVLNIQHSSIKKFGIG-------------------------------LKG 120
             P +F P+ LV LN+  S  KK   G                               L  
Sbjct: 1220 LPKSFNPENLVELNLPSSCAKKLWKGKKARFCTTNSSLEKLKKMRLSYSDQLTKIPRLSS 1279

Query: 121  FKNLRYLNFNGCETLTEIC-DISVMPNLVILDLQQCSNLEFFSSGLKSKSLEVLNLGHCY 180
              NL +++  GC +L  +   IS +  LV L+L+ CS LE   S +  +SLEVLNL  C 
Sbjct: 1280 ATNLEHIDLEGCNSLLSLSQSISYLKKLVFLNLKGCSKLENIPSMVDLESLEVLNLSGCS 1339

Query: 181  KLIKFPDVVEEMECLNIIDLRASG--ITELPTSIENITGLRFLNLDFCEHLVLLPSSIYR 218
            KL  FP++       N+ +L   G  I E+P+SI+N+  L  L+L+   HL  LP+SIY+
Sbjct: 1340 KLGNFPEISP-----NVKELYMGGTMIQEIPSSIKNLVLLEKLDLENSRHLKNLPTSIYK 1399

BLAST of MC02g_new0178 vs. TAIR 10
Match: AT3G44480.1 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 110.5 bits (275), Expect = 2.7e-24
Identity = 70/199 (35.18%), Postives = 108/199 (54.27%), Query Frame = 0

Query: 35  YLSNKLKWLEWAGPCSIAPPSTFYPQKLVVLNIQHSSIKKFGIGLKGFKNLRYLNFNGCE 94
           Y S K++ L W G  S+  PSTF P+ LV L+++ S+++K   G K  +NL++++ +   
Sbjct: 666 YHSPKIRSLNWYGYESLCLPSTFNPEFLVELDMRSSNLRKLWEGTKQLRNLKWMDLSYSS 725

Query: 95  TLTEICDISVMPNLVILDLQQCSNL-EFFSSGLKSKSLEVLNLGHCYKLIKFPDVVEEME 154
            L E+ ++S   NL  L L+ CS+L E  SS  K  SL++L+L +C  L K P +    +
Sbjct: 726 YLKELPNLSTATNLEELKLRNCSSLVELPSSIEKLTSLQILDLENCSSLEKLPAIENATK 785

Query: 155 CLNIIDLRASGITELPTSIENITGLRFLNLDFCEHLVLLPSSIYRLKNLDYIGLNACFRL 214
              +     S + ELP SI   T L+ LN+  C  LV LPSSI  + +L+   L+ C  L
Sbjct: 786 LRELKLQNCSSLIELPLSIGTATNLKQLNISGCSSLVKLPSSIGDITDLEVFDLSNCSSL 845

Query: 215 LKFPQRLNSAVVSSNLLKL 233
           +  P  + +     NL KL
Sbjct: 846 VTLPSSIGNL---QNLCKL 861

BLAST of MC02g_new0178 vs. TAIR 10
Match: AT5G11250.1 (Disease resistance protein (TIR-NBS-LRR class) )

HSP 1 Score: 109.8 bits (273), Expect = 4.7e-24
Identity = 71/200 (35.50%), Postives = 104/200 (52.00%), Query Frame = 0

Query: 35  YLSNKLKWLEWAGPCSIAPPSTFYPQKLVVLNIQHSSIKKFGIGLKGFKNLRYLNFNGCE 94
           YLS KL+ L+W        PST   + L+ LN+ HS +     G+K   NLR ++ +   
Sbjct: 628 YLSRKLQLLDWIYFPMTCLPSTVNVEFLIELNLTHSKLDMLWEGVKPLHNLRQMDLSYSV 687

Query: 95  TLTEICDISVMPNLVILDLQQCSNLEFFSSGL-KSKSLEVLNLGHCYKLIKFPDVVEEME 154
            L E+ D+S   NL  L L  CS+L    S +  + +LE L+L  C  L++ P   + + 
Sbjct: 688 NLKELPDLSTAINLRKLILSNCSSLIKLPSCIGNAINLEDLDLNGCSSLVELPSFGDAIN 747

Query: 155 CLNIIDLRASGITELPTSIENITGLRFLNLDFCEHLVLLPSSIYRLKNLDYIGLNACFRL 214
              ++    S + ELP+SI N   LR L+L +C  L+ LPSSI    NL  + LN C  L
Sbjct: 748 LQKLLLRYCSNLVELPSSIGNAINLRELDLYYCSSLIRLPSSIGNAINLLILDLNGCSNL 807

Query: 215 LKFPQRLNSAVVSSNLLKLD 234
           L+ P  + +A+   NL KLD
Sbjct: 808 LELPSSIGNAI---NLQKLD 824