MC02g_new0101 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC02g_new0101
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Auxin-responsive GH3 family protein
Location	MC02: 4207512 .. 4210032 (+)
RNA-Seq Expression	MC02g_new0101
Synteny	MC02g_new0101

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CTTGCTTATAACTTCATAAATTTGCACCACTTTCCTCCTCCGCACTTCATTTTTTAGAGTTTTTTAGAGCTCAAAGAAATGCCGGAGGCTTCAAACAACTTGCCCCCCGAACCTGATAGCCTAATTGGTAGTTCCCATGAGAAACATGAACGGGCATTGCAGTATATTGAACAAGTTACAGCTCATGCCGATCAAGTTCAACGTCGAGTTCTTACTGAAATTCTCTCCGCAAATGCCCATGTTGAGTACCTACGCCATGGCCTCGACGGATATATGGATACGAAGACGTTCAAGAAGACGATGCCCGTTGTCTCTTACGATGACATAAAACCATACGTCGATCGAATTGCGAATGGCGATCCATCTCCAATACTATGTTCAAAACCCATATCGGAGTTCTTAACCAGGTGAGTATATAATAATTAATCCATTATAGTTTTCATGTTAAACAACATGAATAAATAATTTTTTTGGATATGGTTTTACAACAAGAAGCCCTATATTCACTCTCAAGCACGTACAGAAAATTCTACCTTCTAACTTTTCAAATACATGATCTAATGTAGGGAAAAAACTATTAAAGTTATACTTTTGTATATAATTTTTTAAACTAAGACGAACTAAGAGGGCAATTGCCTCTCCTCCTTTTTCCACTCCGTCTCAGCAAATTCTTATATATCATTTTTTTCTCAATTAATATTGATAGTCAACTTGATTATATTTGTTCGTTTGTAGTTCGGGAACTTCAGGGGGAGAGAGAAAGCTAATGCCAACAACAGAAGAAGAGCTGGAGAGAAAATCATTGCTTTATTCCCTTCTGATGCCTGTGATGAACCAGTTCATTCCAGGGTTGGACAAGGGCAAAGGAATGTACTTTTTATTCGTAAAATCTGAGTCCACAACTCCTGGAGGGCTACTTGCACGTCCAGTTCTCACCAGCTACTACAGAAGCCCACACTTTAAGGACCGCCCCTATGATCCCTACAACCAATACACCAGCCCTAATGAAACAATTCTTTGCACAGATTCATACGAGAGCATGTACTCCCAGTTTCTATGTGGGCTGTGCCTCAGCAGGGAAGTTCTCCGAGTTGGTGCTGTTTTTGCTTCTGGGTTCATTCGTGCCATCAAATTCCTTGAAACTCATTGGCTCCTTTTGTCTAATGATATCAGAACAGGAACCATAAACCCTAGAATCACTGACCCATTAGTGAGACAATCTGTCTTGAACATTCTGAAACCTGACCCTGAGCTTGCGGATCTGATTGAATCTGAGTGCAGTAAAGGGACGTGGGAAGGCATCATAAGTAGGCTGTGGCGGAATACCAAGTATATTGATGTTATTGTGACGGGTACCATGTCACAGTACATCCCAACTTTGGATTACTATAGTAATGGCCTTCCCCTTGTTTGTACTATGTATGCTTCTTCCGAGTGCTACTTTGGCATCAATTTAAATCCCTTGTGTAACCCAAGTGAGGTCTCTTACACCCTCATTCCCACCATGGCGTATTTTGAATTTTTGCCTCTTGATGGGACAAATGGATTTGGCTACTCCAGTACTTGTCAATCAGAGGCCAATCAGAAAACAGACCAAGAGCTGGTTGATCTGGTGGATGTCCAATTAGGAAAAGAATATGAGCTGGTTATCACCACTCATGCAGGAGGGTTTTTAAGTTATAAGTAGTTTTTTTTTTCTAGTTATGCTCGGCTTCTCACAATTTCTTCGTTAAAAACATATTTATTTTACAGGGCTGTATCGATATCGAGTCGGGGACATTCTTCGAGTATCGGGATTCAAGAACAACGCCCCACAGTTCAACTTCGTATGCAGGAAGAATGTGATTCTCAGTATTGATTCGGACAAAGTTGACGAAGTGGAGCTTCAAAACGCAGTAATGGAAGCCTCTAAGAACTTAATTCCATATGGGGCTTGTGTGGTCGAGTACACGAGCTACGCTGACACTGTCACAATTCCAGGCCACTATGTCATCTATTGGGAGATCAGCCTAAAGGAAGCAACCGCCAATAATAATAATGTTATTCCTGGTTCGGTATTTGAAAAATGTTGTCTTTCGATGGAGGAATCACTCAACAGCGTGTACCGCCAAGGAAGAGTCTCAGATAAATCCATTGGTCCTTTAGAGATGAAGATTGTGGAGACAGGGACGTTCGATAAACTCATGGACTATGCCCTCAATCATGGTGCTTCCATAAACCAATACAAGACACCTCGCTGTGTGAAACATGCACCAATTCTCGAGCTTTTGAACTCGTACGTTGTGGACAATTATTTCAGCCCAAAATGTCCCCACTGGGTTCCTGGTCGTAAAGAATGGTCCACCAACAACAGTTAAAAAAAAAAAAAAAAAAAAAAAAGTGTCCACGATTCGGGTTATTGTGTGTGTTTGTGTTTACGTGTGCATTATGTCAAAAACAAAAACAAAAAAGAAAAGGAATAAAATTGTGTTTACTAAAAGGAAGATCATTAACTGTTGTTAGAGTGTCAATGAAATTGGAT

mRNA sequence

ATGCCGGAGGCTTCAAACAACTTGCCCCCCGAACCTGATAGCCTAATTGGTAGTTCCCATGAGAAACATGAACGGGCATTGCAGTATATTGAACAAGTTACAGCTCATGCCGATCAAGTTCAACGTCGAGTTCTTACTGAAATTCTCTCCGCAAATGCCCATGTTGAGTACCTACGCCATGGCCTCGACGGATATATGGATACGAAGACGTTCAAGAAGACGATGCCCGTTGTCTCTTACGATGACATAAAACCATACGTCGATCGAATTGCGAATGGCGATCCATCTCCAATACTATGTTCAAAACCCATATCGGAGTTCTTAACCAGTTCGGGAACTTCAGGGGGAGAGAGAAAGCTAATGCCAACAACAGAAGAAGAGCTGGAGAGAAAATCATTGCTTTATTCCCTTCTGATGCCTGTGATGAACCAGTTCATTCCAGGGTTGGACAAGGGCAAAGGAATGTACTTTTTATTCGTAAAATCTGAGTCCACAACTCCTGGAGGGCTACTTGCACGTCCAGTTCTCACCAGCTACTACAGAAGCCCACACTTTAAGGACCGCCCCTATGATCCCTACAACCAATACACCAGCCCTAATGAAACAATTCTTTGCACAGATTCATACGAGAGCATGTACTCCCAGTTTCTATGTGGGCTGTGCCTCAGCAGGGAAGTTCTCCGAGTTGGTGCTGTTTTTGCTTCTGGGTTCATTCGTGCCATCAAATTCCTTGAAACTCATTGGCTCCTTTTGTCTAATGATATCAGAACAGGAACCATAAACCCTAGAATCACTGACCCATTAGTGAGACAATCTGTCTTGAACATTCTGAAACCTGACCCTGAGCTTGCGGATCTGATTGAATCTGAGTGCAGTAAAGGGACGTGGGAAGGCATCATAAGTAGGCTGTGGCGGAATACCAAGTATATTGATGTTATTGTGACGGGTACCATGTCACAGTACATCCCAACTTTGGATTACTATAGTAATGGCCTTCCCCTTGTTTGTACTATGTATGCTTCTTCCGAGTGCTACTTTGGCATCAATTTAAATCCCTTGTGTAACCCAAGTGAGGTCTCTTACACCCTCATTCCCACCATGGCGTATTTTGAATTTTTGCCTCTTGATGGGACAAATGGATTTGGCTACTCCAGTACTTGTCAATCAGAGGCCAATCAGAAAACAGACCAAGAGCTGGTTGATCTGGTGGATGTCCAATTAGGAAAAGAATATGAGCTGGTTATCACCACTCATGCAGGAGGGTTTTTAAGTTATAAGTAG

Coding sequence (CDS)

ATGCCGGAGGCTTCAAACAACTTGCCCCCCGAACCTGATAGCCTAATTGGTAGTTCCCATGAGAAACATGAACGGGCATTGCAGTATATTGAACAAGTTACAGCTCATGCCGATCAAGTTCAACGTCGAGTTCTTACTGAAATTCTCTCCGCAAATGCCCATGTTGAGTACCTACGCCATGGCCTCGACGGATATATGGATACGAAGACGTTCAAGAAGACGATGCCCGTTGTCTCTTACGATGACATAAAACCATACGTCGATCGAATTGCGAATGGCGATCCATCTCCAATACTATGTTCAAAACCCATATCGGAGTTCTTAACCAGTTCGGGAACTTCAGGGGGAGAGAGAAAGCTAATGCCAACAACAGAAGAAGAGCTGGAGAGAAAATCATTGCTTTATTCCCTTCTGATGCCTGTGATGAACCAGTTCATTCCAGGGTTGGACAAGGGCAAAGGAATGTACTTTTTATTCGTAAAATCTGAGTCCACAACTCCTGGAGGGCTACTTGCACGTCCAGTTCTCACCAGCTACTACAGAAGCCCACACTTTAAGGACCGCCCCTATGATCCCTACAACCAATACACCAGCCCTAATGAAACAATTCTTTGCACAGATTCATACGAGAGCATGTACTCCCAGTTTCTATGTGGGCTGTGCCTCAGCAGGGAAGTTCTCCGAGTTGGTGCTGTTTTTGCTTCTGGGTTCATTCGTGCCATCAAATTCCTTGAAACTCATTGGCTCCTTTTGTCTAATGATATCAGAACAGGAACCATAAACCCTAGAATCACTGACCCATTAGTGAGACAATCTGTCTTGAACATTCTGAAACCTGACCCTGAGCTTGCGGATCTGATTGAATCTGAGTGCAGTAAAGGGACGTGGGAAGGCATCATAAGTAGGCTGTGGCGGAATACCAAGTATATTGATGTTATTGTGACGGGTACCATGTCACAGTACATCCCAACTTTGGATTACTATAGTAATGGCCTTCCCCTTGTTTGTACTATGTATGCTTCTTCCGAGTGCTACTTTGGCATCAATTTAAATCCCTTGTGTAACCCAAGTGAGGTCTCTTACACCCTCATTCCCACCATGGCGTATTTTGAATTTTTGCCTCTTGATGGGACAAATGGATTTGGCTACTCCAGTACTTGTCAATCAGAGGCCAATCAGAAAACAGACCAAGAGCTGGTTGATCTGGTGGATGTCCAATTAGGAAAAGAATATGAGCTGGTTATCACCACTCATGCAGGAGGGTTTTTAAGTTATAAGTAG

Protein sequence

MPEASNNLPPEPDSLIGSSHEKHERALQYIEQVTAHADQVQRRVLTEILSANAHVEYLRHGLDGYMDTKTFKKTMPVVSYDDIKPYVDRIANGDPSPILCSKPISEFLTSSGTSGGERKLMPTTEEELERKSLLYSLLMPVMNQFIPGLDKGKGMYFLFVKSESTTPGGLLARPVLTSYYRSPHFKDRPYDPYNQYTSPNETILCTDSYESMYSQFLCGLCLSREVLRVGAVFASGFIRAIKFLETHWLLLSNDIRTGTINPRITDPLVRQSVLNILKPDPELADLIESECSKGTWEGIISRLWRNTKYIDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGINLNPLCNPSEVSYTLIPTMAYFEFLPLDGTNGFGYSSTCQSEANQKTDQELVDLVDVQLGKEYELVITTHAGGFLSYK

Homology

[Close]

BLAST of MC02g_new0101 vs. ExPASy Swiss-Prot
Match: Q9LSQ4 (Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana OX=3702 GN=GH3.6 PE=1 SV=1)

HSP 1 Score: 614.8 bits (1584), Expect = 7.5e-175
Identity = 299/401 (74.56%), Postives = 342/401 (85.29%), Query Frame = 0


Query: 21  EKHERALQYIEQVTAHADQVQRRVLTEILSANAHVEYL-RHGLDGYMDTKTFKKTMPVVS 80
           EK++  LQ+IE VT +AD VQRRVL EILS NA VEYL RHGL+G  D +TFK  MPVV+
Sbjct: 19  EKNKNKLQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHGLEGRTDRETFKHIMPVVT 78

Query: 81  YDDIKPYVDRIANGDPSPILCSKPISEFLTSSGTSGGERKLMPTTEEELERKSLLYSLLM 140
           Y+DI+P ++RIANGD S +LCS PISEFLTSSGTSGGERKLMPT EEEL+R+SLLYSLLM
Sbjct: 79  YEDIQPEINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSLLM 138

Query: 141 PVMNQFIPGLDKGKGMYFLFVKSESTTPGGLLARPVLTSYYRSPHFKDRPYDPYNQYTSP 200
           PVM+QF+PGLDKGKGMYFLF+KSES TPGGL ARPVLTSYY+S HFK+RPYDPY  YTSP
Sbjct: 139 PVMDQFVPGLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKNRPYDPYTNYTSP 198

Query: 201 NETILCTDSYESMYSQFLCGLCLSREVLRVGAVFASGFIRAIKFLETHWLLLSNDIRTGT 260
           N+TILC+DSY+SMYSQ LCGLC  +EVLRVGAVFASGFIRAIKFLE HW  L+ DIRTGT
Sbjct: 199 NQTILCSDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIKFLEKHWPELARDIRTGT 258

Query: 261 INPRITDPLVRQSVLNILKPDPELADLIESECSKGTWEGIISRLWRNTKYIDVIVTGTMS 320
           ++  ITD  VR++V  ILKPDP+LAD +ESEC K +W+GII+RLW NTKY+DVIVTGTMS
Sbjct: 259 LSSEITDSSVREAVGEILKPDPKLADFVESECRKTSWQGIITRLWPNTKYVDVIVTGTMS 318

Query: 321 QYIPTLDYYSNGLPLVCTMYASSECYFGINLNPLCNPSEVSYTLIPTMAYFEFLPLDGTN 380
           QYIPTLDYYSNGLPLVCTMYASSECYFG+NL PLC PSEVSYTLIP MAYFEFLP+   +
Sbjct: 319 QYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPNMAYFEFLPVHRNS 378

Query: 381 GFGYSSTCQSEANQKTDQELVDLVDVQLGKEYELVITTHAG 421
           G   S +      +K  QELVDLVDV+LG+EYELV+TT+AG
Sbjct: 379 GVTSSISLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAG 419

[Close]

BLAST of MC02g_new0101 vs. ExPASy Swiss-Prot
Match: O81829 (Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana OX=3702 GN=GH3.5 PE=1 SV=1)

HSP 1 Score: 609.8 bits (1571), Expect = 2.4e-173
Identity = 301/421 (71.50%), Postives = 350/421 (83.14%), Query Frame = 0


Query: 1   MPEASNNLPPEPDSLIGSSHEKHERALQYIEQVTAHADQVQRRVLTEILSANAHVEYL-R 60
           MPEA      E   L  +  +K+++ LQ IE++T++ADQVQR+VL EIL+ NA VEYL R
Sbjct: 1   MPEAPKKESLEVFDL--TLDQKNKQKLQLIEELTSNADQVQRQVLEEILTRNADVEYLRR 60

Query: 61  HGLDGYMDTKTFKKTMPVVSYDDIKPYVDRIANGDPSPILCSKPISEFLTSSGTSGGERK 120
           H L+G  D +TFK  MPV++Y+DI+P ++RIANGD SPIL SKPISEFLTSSGTSGGERK
Sbjct: 61  HDLNGRTDRETFKNIMPVITYEDIEPEINRIANGDKSPILSSKPISEFLTSSGTSGGERK 120

Query: 121 LMPTTEEELERKSLLYSLLMPVMNQFIPGLDKGKGMYFLFVKSESTTPGGLLARPVLTSY 180
           LMPT EEEL+R+SLLYSLLMPVM+QF+PGL+ GKGMYFLF+KSES TPGGL ARPVLTSY
Sbjct: 121 LMPTIEEELDRRSLLYSLLMPVMSQFVPGLENGKGMYFLFIKSESKTPGGLPARPVLTSY 180

Query: 181 YRSPHFKDRPYDPYNQYTSPNETILCTDSYESMYSQFLCGLCLSREVLRVGAVFASGFIR 240
           Y+S HFK+RPYDPY  YTSPNETILC+DSY+SMYSQ LCGLC  +EVLRVGAVFASGFIR
Sbjct: 181 YKSSHFKERPYDPYTNYTSPNETILCSDSYQSMYSQMLCGLCQHQEVLRVGAVFASGFIR 240

Query: 241 AIKFLETHWLLLSNDIRTGTINPRITDPLVRQSVLNILKPDPELADLIESECSKGTWEGI 300
           AIKFLE HW+ L  DIRTGT++  ITDP VR++V  ILKP P+LAD +E EC K +W+GI
Sbjct: 241 AIKFLEKHWIELVRDIRTGTLSSLITDPSVREAVAKILKPSPKLADFVEFECKKSSWQGI 300

Query: 301 ISRLWRNTKYIDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGINLNPLCNPSEV 360
           I+RLW NTKY+DVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFG+NL PLC PSEV
Sbjct: 301 ITRLWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEV 360

Query: 361 SYTLIPTMAYFEFLPLDGTNGFGYSSTCQSEANQKTDQELVDLVDVQLGKEYELVITTHA 420
           SYTLIP+MAYFEFLP+   NG   S        +K  QELVDLVDV+LG+EYELV+TT+A
Sbjct: 361 SYTLIPSMAYFEFLPVHRNNGVTNSINLPKALTEKEQQELVDLVDVKLGQEYELVVTTYA 419

[Close]

BLAST of MC02g_new0101 vs. ExPASy Swiss-Prot
Match: A3BLS0 (Indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. indica OX=39946 GN=GH3.8 PE=1 SV=2)

HSP 1 Score: 531.9 bits (1369), Expect = 6.4e-150
Identity = 254/405 (62.72%), Postives = 317/405 (78.27%), Query Frame = 0


Query: 17  GSSHEKHERALQYIEQVTAHADQVQRRVLTEILSANAHVEYL-RHGLDGYMDTKTFKKTM 76
           G+  E     L++I+++T + D VQ RVL EIL  NA  EYL + GLDG  D   F+  +
Sbjct: 20  GAVKEGDVEKLRFIDEMTTNVDAVQERVLGEILGRNAGTEYLTKCGLDGATDRAAFRAKV 79

Query: 77  PVVSYDDIKPYVDRIANGDPSPILCSKPISEFLTSSGTSGGERKLMPTTEEELERKSLLY 136
           PVVSYDD++PY+ RIANGD SPIL + P+SEFLTSSGTS GERKLMPT  +EL+R+ LLY
Sbjct: 80  PVVSYDDLQPYIQRIANGDRSPILSTHPVSEFLTSSGTSAGERKLMPTIMDELDRRQLLY 139

Query: 137 SLLMPVMNQFIPGLDKGKGMYFLFVKSESTTPGGLLARPVLTSYYRSPHFKDRPYDPYNQ 196
           SLLMPVMN ++PGLDKGKG+YFLFVKSE+ TPGGL ARPVLTSYY+S HFK+RPYDPY+ 
Sbjct: 140 SLLMPVMNLYVPGLDKGKGLYFLFVKSETKTPGGLTARPVLTSYYKSDHFKNRPYDPYHN 199

Query: 197 YTSPNETILCTDSYESMYSQFLCGLCLSREVLRVGAVFASGFIRAIKFLETHWLLLSNDI 256
           YTSP   ILC D+++SMY+Q +CGLC   +VLR+GAVFASG +RAI+FL+ +W  L++DI
Sbjct: 200 YTSPTAAILCADAFQSMYAQMVCGLCQRNDVLRLGAVFASGLLRAIRFLQLNWEQLADDI 259

Query: 257 RTGTINPRITDPLVRQSVLNILKPDPELADLIESECSKGTWEGIISRLWRNTKYIDVIVT 316
            +G + PR+TDP VR++V  IL PDPELA LI +ECSKG W GII+R+W NTKY+DVIVT
Sbjct: 260 ESGELTPRVTDPSVREAVAAILLPDPELAKLIRAECSKGDWAGIITRVWPNTKYLDVIVT 319

Query: 317 GTMSQYIPTLDYYSNGLPLVCTMYASSECYFGINLNPLCNPSEVSYTLIPTMAYFEFLPL 376
           G M+QYIPTL++YS GLP+ CTMYASSECYFG+NL P+C+PSEVSYT++P M YFEFLP+
Sbjct: 320 GAMAQYIPTLEFYSGGLPMACTMYASSECYFGLNLRPMCDPSEVSYTIMPNMGYFEFLPV 379

Query: 377 DGTNGFGYSSTCQSEANQKTDQELVDLVDVQLGKEYELVITTHAG 421
           D T      +T           +LVDL  V++G+EYELVITT+AG
Sbjct: 380 DETGAASGDAT-----------QLVDLARVEVGREYELVITTYAG 413

[Close]

BLAST of MC02g_new0101 vs. ExPASy Swiss-Prot
Match: Q0D4Z6 (Indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.8 PE=1 SV=1)

HSP 1 Score: 531.9 bits (1369), Expect = 6.4e-150
Identity = 254/405 (62.72%), Postives = 317/405 (78.27%), Query Frame = 0


Query: 17  GSSHEKHERALQYIEQVTAHADQVQRRVLTEILSANAHVEYL-RHGLDGYMDTKTFKKTM 76
           G+  E     L++I+++T + D VQ RVL EIL  NA  EYL + GLDG  D   F+  +
Sbjct: 20  GAVKEGDVEKLRFIDEMTTNVDAVQERVLGEILGRNAGTEYLTKCGLDGATDRAAFRAKV 79

Query: 77  PVVSYDDIKPYVDRIANGDPSPILCSKPISEFLTSSGTSGGERKLMPTTEEELERKSLLY 136
           PVVSYDD++PY+ RIANGD SPIL + P+SEFLTSSGTS GERKLMPT  +EL+R+ LLY
Sbjct: 80  PVVSYDDLQPYIQRIANGDRSPILSTHPVSEFLTSSGTSAGERKLMPTIMDELDRRQLLY 139

Query: 137 SLLMPVMNQFIPGLDKGKGMYFLFVKSESTTPGGLLARPVLTSYYRSPHFKDRPYDPYNQ 196
           SLLMPVMN ++PGLDKGKG+YFLFVKSE+ TPGGL ARPVLTSYY+S HFK+RPYDPY+ 
Sbjct: 140 SLLMPVMNLYVPGLDKGKGLYFLFVKSETKTPGGLTARPVLTSYYKSDHFKNRPYDPYHN 199

Query: 197 YTSPNETILCTDSYESMYSQFLCGLCLSREVLRVGAVFASGFIRAIKFLETHWLLLSNDI 256
           YTSP   ILC D+++SMY+Q +CGLC   +VLR+GAVFASG +RAI+FL+ +W  L++DI
Sbjct: 200 YTSPTAAILCADAFQSMYAQMVCGLCQRNDVLRLGAVFASGLLRAIRFLQLNWEQLADDI 259

Query: 257 RTGTINPRITDPLVRQSVLNILKPDPELADLIESECSKGTWEGIISRLWRNTKYIDVIVT 316
            +G + PR+TDP VR++V  IL PDPELA LI +ECSKG W GII+R+W NTKY+DVIVT
Sbjct: 260 ESGELTPRVTDPSVREAVAAILLPDPELAKLIRAECSKGDWAGIITRVWPNTKYLDVIVT 319

Query: 317 GTMSQYIPTLDYYSNGLPLVCTMYASSECYFGINLNPLCNPSEVSYTLIPTMAYFEFLPL 376
           G M+QYIPTL++YS GLP+ CTMYASSECYFG+NL P+C+PSEVSYT++P M YFEFLP+
Sbjct: 320 GAMAQYIPTLEFYSGGLPMACTMYASSECYFGLNLRPMCDPSEVSYTIMPNMGYFEFLPV 379

Query: 377 DGTNGFGYSSTCQSEANQKTDQELVDLVDVQLGKEYELVITTHAG 421
           D T      +T           +LVDL  V++G+EYELVITT+AG
Sbjct: 380 DETGAASGDAT-----------QLVDLARVEVGREYELVITTYAG 413

[Close]

BLAST of MC02g_new0101 vs. ExPASy Swiss-Prot
Match: Q8LQM5 (Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.1 PE=2 SV=1)

HSP 1 Score: 525.8 bits (1353), Expect = 4.6e-148
Identity = 259/400 (64.75%), Postives = 313/400 (78.25%), Query Frame = 0


Query: 23  HERALQYIEQVTAHADQVQRRVLTEILSANAHVEYL-RHGLDGYMD-TKTFKKTMPVVSY 82
           H  AL++IE VTA+A QVQRRVL EIL+ NA  EYL R+G+ G  D    F++ +P+V+Y
Sbjct: 20  HAEALEFIEHVTANAGQVQRRVLGEILAQNAPAEYLRRYGIPGSPDVVDAFRRLVPLVTY 79

Query: 83  DDIKPYVDRIANGDPSPILCSKPISEFLTSSGTSGGERKLMPTTEEELERKSLLYSLLMP 142
           + ++P + RIANGD SPI   KPISEFLTSSGTSGGERKLMPT  +E+ R+SLLYSLLMP
Sbjct: 80  EGLQPDILRIANGDTSPIFSGKPISEFLTSSGTSGGERKLMPTIADEMNRRSLLYSLLMP 139

Query: 143 VMNQFIPGLDKGKGMYFLFVKSESTTPGGLLARPVLTSYYRSPHFKDRPYDPYNQYTSPN 202
           VM+Q + GLDKGK MY LFVK+ES TPGGL ARPVLTSYYRS  F DRP DPY  YTSP+
Sbjct: 140 VMSQSVSGLDKGKAMYLLFVKAESRTPGGLAARPVLTSYYRSRQFLDRPRDPYTSYTSPD 199

Query: 203 ETILCTDSYESMYSQFLCGLCLSREVLRVGAVFASGFIRAIKFLETHWLLLSNDIRTGTI 262
           E ILC DSY+SMY+Q LCGL    +VLRVGAVFASGF+RAI FLE HW  L +DIRTG +
Sbjct: 200 EAILCVDSYQSMYAQLLCGLVHRADVLRVGAVFASGFLRAIHFLEKHWARLCHDIRTGEL 259

Query: 263 NPRITDPLVRQSVLNILKPDPELADLIESECSKGTWEGIISRLWRNTKYIDVIVTGTMSQ 322
           +P ITD +VR +V  +L+ DP LAD IE EC++ +WEGII RLW  TKYIDVIVTGTMSQ
Sbjct: 260 DPEITDRVVRDAVGRVLRADPALADAIEDECARASWEGIIRRLWPRTKYIDVIVTGTMSQ 319

Query: 323 YIPTLDYYSNGLPLVCTMYASSECYFGINLNPLCNPSEVSYTLIPTMAYFEFLPLDGTNG 382
           YIPTL++Y  GLPL CTMYASSECYFG+NLNP+C PS+V+YTLIPTM Y+EFLP++    
Sbjct: 320 YIPTLEFYGGGLPLTCTMYASSECYFGLNLNPMCKPSDVAYTLIPTMCYYEFLPVN---- 379

Query: 383 FGYSSTCQSEANQKTDQELVDLVDVQLGKEYELVITTHAG 421
                 C +   + + ++LVDLVDV+LG EYELV+TT++G
Sbjct: 380 ------CNNATAEASHRDLVDLVDVKLGHEYELVVTTYSG 409

[Close]

BLAST of MC02g_new0101 vs. NCBI nr
Match: XP_022140599.1 (indole-3-acetic acid-amido synthetase GH3.6-like isoform X2 [Momordica charantia])

HSP 1 Score: 852 bits (2202), Expect = 8.30e-309
Identity = 418/420 (99.52%), Postives = 420/420 (100.00%), Query Frame = 0


Query: 1   MPEASNNLPPEPDSLIGSSHEKHERALQYIEQVTAHADQVQRRVLTEILSANAHVEYLRH 60
           MPEASNNLPPEPDSLIGSSHEKHERALQYIEQVTAHADQVQRRVLTEILSANAHVEYLRH
Sbjct: 1   MPEASNNLPPEPDSLIGSSHEKHERALQYIEQVTAHADQVQRRVLTEILSANAHVEYLRH 60

Query: 61  GLDGYMDTKTFKKTMPVVSYDDIKPYVDRIANGDPSPILCSKPISEFLTSSGTSGGERKL 120
           GLDGYMDTKTFKKTMPVVSYDDIKPYVDRIANGDPSPILCSKPISEFLTSSGTSGGERKL
Sbjct: 61  GLDGYMDTKTFKKTMPVVSYDDIKPYVDRIANGDPSPILCSKPISEFLTSSGTSGGERKL 120

Query: 121 MPTTEEELERKSLLYSLLMPVMNQFIPGLDKGKGMYFLFVKSESTTPGGLLARPVLTSYY 180
           MPTTEEELERKSLLYSLLMPVMNQFIPGLDKGKGMYFLFVKSESTTPGGLLARPVLTSYY
Sbjct: 121 MPTTEEELERKSLLYSLLMPVMNQFIPGLDKGKGMYFLFVKSESTTPGGLLARPVLTSYY 180

Query: 181 RSPHFKDRPYDPYNQYTSPNETILCTDSYESMYSQFLCGLCLSREVLRVGAVFASGFIRA 240
           RSPHFKDRPYDPYNQYTSPNETILCTDSY+SMYSQFLCGLCLSREVLRVGAVFASGFIRA
Sbjct: 181 RSPHFKDRPYDPYNQYTSPNETILCTDSYQSMYSQFLCGLCLSREVLRVGAVFASGFIRA 240

Query: 241 IKFLETHWLLLSNDIRTGTINPRITDPLVRQSVLNILKPDPELADLIESECSKGTWEGII 300
           IKFLETHWLLLSNDIRTGTINPRITDPLVRQSVLNILKPDPELADLIESECSKGTWEGII
Sbjct: 241 IKFLETHWLLLSNDIRTGTINPRITDPLVRQSVLNILKPDPELADLIESECSKGTWEGII 300

Query: 301 SRLWRNTKYIDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGINLNPLCNPSEVS 360
           SRLWRNTKYIDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGINLNPLCNPSEVS
Sbjct: 301 SRLWRNTKYIDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGINLNPLCNPSEVS 360

Query: 361 YTLIPTMAYFEFLPLDGTNGFGYSSTCQSEANQKTDQELVDLVDVQLGKEYELVITTHAG 420
           YTLIPTMAYFEFLPLDGTNGFGYSSTCQSEANQKTDQELVDLVDVQLGKEYELVITT+AG
Sbjct: 361 YTLIPTMAYFEFLPLDGTNGFGYSSTCQSEANQKTDQELVDLVDVQLGKEYELVITTYAG 420

[Close]

BLAST of MC02g_new0101 vs. NCBI nr
Match: XP_022140598.1 (indole-3-acetic acid-amido synthetase GH3.6-like isoform X1 [Momordica charantia])

HSP 1 Score: 665 bits (1716), Expect = 2.70e-236
Identity = 327/328 (99.70%), Postives = 328/328 (100.00%), Query Frame = 0


Query: 1   MPEASNNLPPEPDSLIGSSHEKHERALQYIEQVTAHADQVQRRVLTEILSANAHVEYLRH 60
           MPEASNNLPPEPDSLIGSSHEKHERALQYIEQVTAHADQVQRRVLTEILSANAHVEYLRH
Sbjct: 1   MPEASNNLPPEPDSLIGSSHEKHERALQYIEQVTAHADQVQRRVLTEILSANAHVEYLRH 60

Query: 61  GLDGYMDTKTFKKTMPVVSYDDIKPYVDRIANGDPSPILCSKPISEFLTSSGTSGGERKL 120
           GLDGYMDTKTFKKTMPVVSYDDIKPYVDRIANGDPSPILCSKPISEFLTSSGTSGGERKL
Sbjct: 61  GLDGYMDTKTFKKTMPVVSYDDIKPYVDRIANGDPSPILCSKPISEFLTSSGTSGGERKL 120

Query: 121 MPTTEEELERKSLLYSLLMPVMNQFIPGLDKGKGMYFLFVKSESTTPGGLLARPVLTSYY 180
           MPTTEEELERKSLLYSLLMPVMNQFIPGLDKGKGMYFLFVKSESTTPGGLLARPVLTSYY
Sbjct: 121 MPTTEEELERKSLLYSLLMPVMNQFIPGLDKGKGMYFLFVKSESTTPGGLLARPVLTSYY 180

Query: 181 RSPHFKDRPYDPYNQYTSPNETILCTDSYESMYSQFLCGLCLSREVLRVGAVFASGFIRA 240
           RSPHFKDRPYDPYNQYTSPNETILCTDSY+SMYSQFLCGLCLSREVLRVGAVFASGFIRA
Sbjct: 181 RSPHFKDRPYDPYNQYTSPNETILCTDSYQSMYSQFLCGLCLSREVLRVGAVFASGFIRA 240

Query: 241 IKFLETHWLLLSNDIRTGTINPRITDPLVRQSVLNILKPDPELADLIESECSKGTWEGII 300
           IKFLETHWLLLSNDIRTGTINPRITDPLVRQSVLNILKPDPELADLIESECSKGTWEGII
Sbjct: 241 IKFLETHWLLLSNDIRTGTINPRITDPLVRQSVLNILKPDPELADLIESECSKGTWEGII 300

Query: 301 SRLWRNTKYIDVIVTGTMSQYIPTLDYY 328
           SRLWRNTKYIDVIVTGTMSQYIPTLDYY
Sbjct: 301 SRLWRNTKYIDVIVTGTMSQYIPTLDYY 328

[Close]

BLAST of MC02g_new0101 vs. NCBI nr
Match: KAG7034947.1 (Indole-3-acetic acid-amido synthetase GH3.6, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 659 bits (1700), Expect = 3.27e-233
Identity = 323/395 (81.77%), Postives = 354/395 (89.62%), Query Frame = 0


Query: 27  LQYIEQVTAHADQVQRRVLTEILSANAHVEYLRH-GLDGYMDTKTFKKTMPVVSYDDIKP 86
           LQ+IE++T HADQVQRRVLTEILS NAHVEYL+  GLDG MD   FK  +PVVSY+++KP
Sbjct: 10  LQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVPVVSYENLKP 69

Query: 87  YVDRIANGDPSPILCSKPISEFLTSSGTSGGERKLMPTTEEELERKSLLYSLLMPVMNQF 146
           Y+DRIANGD SPILCS+P+SEFLTSSGTSGGERKLMPTTEEEL+R+S LYSLLMPVMNQF
Sbjct: 70  YIDRIANGDASPILCSEPVSEFLTSSGTSGGERKLMPTTEEELKRRSFLYSLLMPVMNQF 129

Query: 147 IPGLDKGKGMYFLFVKSESTTPGGLLARPVLTSYYRSPHFKDRPYDPYNQYTSPNETILC 206
           IPGL+KGKGMYFLFVKSESTTPGGL ARPVLTSYY+S HFKDRPYD Y  YTSPN+TILC
Sbjct: 130 IPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSPNQTILC 189

Query: 207 TDSYESMYSQFLCGLCLSREVLRVGAVFASGFIRAIKFLETHWLLLSNDIRTGTINPRIT 266
           TDSY+SMY QFLCGLCLS++VLRVG+VFASGFIRAIKFLETHW+LL+NDIRTGTINPRI+
Sbjct: 190 TDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIRTGTINPRIS 249

Query: 267 DPLVRQSVLNILKPDPELADLIESECSKGTWEGIISRLWRNTKYIDVIVTGTMSQYIPTL 326
           DPLVRQSV+NILKPDP LADLIE ECSKGTW GII RLW NTKYIDVIVTGTMSQYIPTL
Sbjct: 250 DPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIIPRLWPNTKYIDVIVTGTMSQYIPTL 309

Query: 327 DYYSNGLPLVCTMYASSECYFGINLNPLCNPSEVSYTLIPTMAYFEFLPLDGTNGFGYSS 386
           DYYSNGLPLVCTMYASSECYFG+NLNPLCNP+EVSYTLIPTMAYFEFLPLD         
Sbjct: 310 DYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLD--------- 369

Query: 387 TCQSEANQKTDQELVDLVDVQLGKEYELVITTHAG 420
                  +K  +ELVDLVDV+LGKEYELVITT++G
Sbjct: 370 -------RKMSEELVDLVDVELGKEYELVITTYSG 388

[Close]

BLAST of MC02g_new0101 vs. NCBI nr
Match: XP_022948165.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita moschata])

HSP 1 Score: 658 bits (1698), Expect = 1.04e-232
Identity = 323/395 (81.77%), Postives = 353/395 (89.37%), Query Frame = 0


Query: 27  LQYIEQVTAHADQVQRRVLTEILSANAHVEYLRH-GLDGYMDTKTFKKTMPVVSYDDIKP 86
           LQ+IE++T HADQVQRRVLTEILS NAHVEYL+  GLDG MD   FK  +PVVSY+++KP
Sbjct: 10  LQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVPVVSYENLKP 69

Query: 87  YVDRIANGDPSPILCSKPISEFLTSSGTSGGERKLMPTTEEELERKSLLYSLLMPVMNQF 146
           Y+DRIANGD SPILCS+P+SEFLTSSGTSGGERKLMPTTEEEL+R+S LYSLLMPVMNQF
Sbjct: 70  YIDRIANGDASPILCSEPVSEFLTSSGTSGGERKLMPTTEEELKRRSFLYSLLMPVMNQF 129

Query: 147 IPGLDKGKGMYFLFVKSESTTPGGLLARPVLTSYYRSPHFKDRPYDPYNQYTSPNETILC 206
           IPGL+KGKGMYFLFVKSESTTPGGL ARPVLTSYY+S HFKDRPYD Y  YTSPN+TILC
Sbjct: 130 IPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSPNQTILC 189

Query: 207 TDSYESMYSQFLCGLCLSREVLRVGAVFASGFIRAIKFLETHWLLLSNDIRTGTINPRIT 266
           TDSY+SMY QFLCGLCLS++VLRVG+VFASGFIRAIKFLETHW+LL+NDIRTGTINPRI 
Sbjct: 190 TDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIRTGTINPRIR 249

Query: 267 DPLVRQSVLNILKPDPELADLIESECSKGTWEGIISRLWRNTKYIDVIVTGTMSQYIPTL 326
           DPLVRQSV+NILKPDP LADLIE ECSKGTW GII RLW NTKYIDVIVTGTMSQYIPTL
Sbjct: 250 DPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIIPRLWPNTKYIDVIVTGTMSQYIPTL 309

Query: 327 DYYSNGLPLVCTMYASSECYFGINLNPLCNPSEVSYTLIPTMAYFEFLPLDGTNGFGYSS 386
           DYYSNGLPLVCTMYASSECYFG+NLNPLCNP+EVSYTLIPTMAYFEFLPLD         
Sbjct: 310 DYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLD--------- 369

Query: 387 TCQSEANQKTDQELVDLVDVQLGKEYELVITTHAG 420
                  +K  +ELVDLVDV+LGKEYELVITT++G
Sbjct: 370 -------RKMSEELVDLVDVELGKEYELVITTYSG 388

[Close]

BLAST of MC02g_new0101 vs. NCBI nr
Match: XP_023533206.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 658 bits (1698), Expect = 1.34e-232
Identity = 322/395 (81.52%), Postives = 355/395 (89.87%), Query Frame = 0


Query: 27  LQYIEQVTAHADQVQRRVLTEILSANAHVEYLRH-GLDGYMDTKTFKKTMPVVSYDDIKP 86
           LQ+IE++T HADQVQRRVLTEILS NAHVEYL+  GLDG MD   FK  +PVVSY+++KP
Sbjct: 17  LQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVPVVSYENLKP 76

Query: 87  YVDRIANGDPSPILCSKPISEFLTSSGTSGGERKLMPTTEEELERKSLLYSLLMPVMNQF 146
           Y+DRIANGD SPILCS+P+SEFLTSSGTSGGERKLMPTTEEEL+R+S LYSLLMPVMNQF
Sbjct: 77  YIDRIANGDASPILCSEPVSEFLTSSGTSGGERKLMPTTEEELKRRSFLYSLLMPVMNQF 136

Query: 147 IPGLDKGKGMYFLFVKSESTTPGGLLARPVLTSYYRSPHFKDRPYDPYNQYTSPNETILC 206
           IPGL++GKGMYFLFVKSESTTPGGL ARPVLTSYY+S HFKDRPYD Y  YTSPN+TILC
Sbjct: 137 IPGLNRGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSPNQTILC 196

Query: 207 TDSYESMYSQFLCGLCLSREVLRVGAVFASGFIRAIKFLETHWLLLSNDIRTGTINPRIT 266
           TDSY+SMYSQFLCGLCLS++VLRVG+VFASGFIRAIKFLETHW+LL+NDIRTGTINPRI+
Sbjct: 197 TDSYQSMYSQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIRTGTINPRIS 256

Query: 267 DPLVRQSVLNILKPDPELADLIESECSKGTWEGIISRLWRNTKYIDVIVTGTMSQYIPTL 326
           DPLVRQSV+NILKPDP LADLIE ECSKGTW GII RLW NTKYIDVIVTGTMSQYIPTL
Sbjct: 257 DPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIIPRLWPNTKYIDVIVTGTMSQYIPTL 316

Query: 327 DYYSNGLPLVCTMYASSECYFGINLNPLCNPSEVSYTLIPTMAYFEFLPLDGTNGFGYSS 386
           DYYSNGLPLVCTMYASSECYFG+NLNPLCNP+EVSYTLIPTMAYFEFLPLD         
Sbjct: 317 DYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLD--------- 376

Query: 387 TCQSEANQKTDQELVDLVDVQLGKEYELVITTHAG 420
                  ++  +ELVDLVDV+LGKEYELVITT++G
Sbjct: 377 -------REMSEELVDLVDVELGKEYELVITTYSG 395

[Close]

BLAST of MC02g_new0101 vs. ExPASy TrEMBL
Match: A0A6J1CG47 (indole-3-acetic acid-amido synthetase GH3.6-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111011212 PE=3 SV=1)

HSP 1 Score: 852 bits (2202), Expect = 4.02e-309
Identity = 418/420 (99.52%), Postives = 420/420 (100.00%), Query Frame = 0


Query: 1   MPEASNNLPPEPDSLIGSSHEKHERALQYIEQVTAHADQVQRRVLTEILSANAHVEYLRH 60
           MPEASNNLPPEPDSLIGSSHEKHERALQYIEQVTAHADQVQRRVLTEILSANAHVEYLRH
Sbjct: 1   MPEASNNLPPEPDSLIGSSHEKHERALQYIEQVTAHADQVQRRVLTEILSANAHVEYLRH 60

Query: 61  GLDGYMDTKTFKKTMPVVSYDDIKPYVDRIANGDPSPILCSKPISEFLTSSGTSGGERKL 120
           GLDGYMDTKTFKKTMPVVSYDDIKPYVDRIANGDPSPILCSKPISEFLTSSGTSGGERKL
Sbjct: 61  GLDGYMDTKTFKKTMPVVSYDDIKPYVDRIANGDPSPILCSKPISEFLTSSGTSGGERKL 120

Query: 121 MPTTEEELERKSLLYSLLMPVMNQFIPGLDKGKGMYFLFVKSESTTPGGLLARPVLTSYY 180
           MPTTEEELERKSLLYSLLMPVMNQFIPGLDKGKGMYFLFVKSESTTPGGLLARPVLTSYY
Sbjct: 121 MPTTEEELERKSLLYSLLMPVMNQFIPGLDKGKGMYFLFVKSESTTPGGLLARPVLTSYY 180

Query: 181 RSPHFKDRPYDPYNQYTSPNETILCTDSYESMYSQFLCGLCLSREVLRVGAVFASGFIRA 240
           RSPHFKDRPYDPYNQYTSPNETILCTDSY+SMYSQFLCGLCLSREVLRVGAVFASGFIRA
Sbjct: 181 RSPHFKDRPYDPYNQYTSPNETILCTDSYQSMYSQFLCGLCLSREVLRVGAVFASGFIRA 240

Query: 241 IKFLETHWLLLSNDIRTGTINPRITDPLVRQSVLNILKPDPELADLIESECSKGTWEGII 300
           IKFLETHWLLLSNDIRTGTINPRITDPLVRQSVLNILKPDPELADLIESECSKGTWEGII
Sbjct: 241 IKFLETHWLLLSNDIRTGTINPRITDPLVRQSVLNILKPDPELADLIESECSKGTWEGII 300

Query: 301 SRLWRNTKYIDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGINLNPLCNPSEVS 360
           SRLWRNTKYIDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGINLNPLCNPSEVS
Sbjct: 301 SRLWRNTKYIDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGINLNPLCNPSEVS 360

Query: 361 YTLIPTMAYFEFLPLDGTNGFGYSSTCQSEANQKTDQELVDLVDVQLGKEYELVITTHAG 420
           YTLIPTMAYFEFLPLDGTNGFGYSSTCQSEANQKTDQELVDLVDVQLGKEYELVITT+AG
Sbjct: 361 YTLIPTMAYFEFLPLDGTNGFGYSSTCQSEANQKTDQELVDLVDVQLGKEYELVITTYAG 420

[Close]

BLAST of MC02g_new0101 vs. ExPASy TrEMBL
Match: A0A6J1CIC6 (indole-3-acetic acid-amido synthetase GH3.6-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111011212 PE=3 SV=1)

HSP 1 Score: 665 bits (1716), Expect = 1.31e-236
Identity = 327/328 (99.70%), Postives = 328/328 (100.00%), Query Frame = 0


Query: 1   MPEASNNLPPEPDSLIGSSHEKHERALQYIEQVTAHADQVQRRVLTEILSANAHVEYLRH 60
           MPEASNNLPPEPDSLIGSSHEKHERALQYIEQVTAHADQVQRRVLTEILSANAHVEYLRH
Sbjct: 1   MPEASNNLPPEPDSLIGSSHEKHERALQYIEQVTAHADQVQRRVLTEILSANAHVEYLRH 60

Query: 61  GLDGYMDTKTFKKTMPVVSYDDIKPYVDRIANGDPSPILCSKPISEFLTSSGTSGGERKL 120
           GLDGYMDTKTFKKTMPVVSYDDIKPYVDRIANGDPSPILCSKPISEFLTSSGTSGGERKL
Sbjct: 61  GLDGYMDTKTFKKTMPVVSYDDIKPYVDRIANGDPSPILCSKPISEFLTSSGTSGGERKL 120

Query: 121 MPTTEEELERKSLLYSLLMPVMNQFIPGLDKGKGMYFLFVKSESTTPGGLLARPVLTSYY 180
           MPTTEEELERKSLLYSLLMPVMNQFIPGLDKGKGMYFLFVKSESTTPGGLLARPVLTSYY
Sbjct: 121 MPTTEEELERKSLLYSLLMPVMNQFIPGLDKGKGMYFLFVKSESTTPGGLLARPVLTSYY 180

Query: 181 RSPHFKDRPYDPYNQYTSPNETILCTDSYESMYSQFLCGLCLSREVLRVGAVFASGFIRA 240
           RSPHFKDRPYDPYNQYTSPNETILCTDSY+SMYSQFLCGLCLSREVLRVGAVFASGFIRA
Sbjct: 181 RSPHFKDRPYDPYNQYTSPNETILCTDSYQSMYSQFLCGLCLSREVLRVGAVFASGFIRA 240

Query: 241 IKFLETHWLLLSNDIRTGTINPRITDPLVRQSVLNILKPDPELADLIESECSKGTWEGII 300
           IKFLETHWLLLSNDIRTGTINPRITDPLVRQSVLNILKPDPELADLIESECSKGTWEGII
Sbjct: 241 IKFLETHWLLLSNDIRTGTINPRITDPLVRQSVLNILKPDPELADLIESECSKGTWEGII 300

Query: 301 SRLWRNTKYIDVIVTGTMSQYIPTLDYY 328
           SRLWRNTKYIDVIVTGTMSQYIPTLDYY
Sbjct: 301 SRLWRNTKYIDVIVTGTMSQYIPTLDYY 328

[Close]

BLAST of MC02g_new0101 vs. ExPASy TrEMBL
Match: A0A6J1G8Z2 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita moschata OX=3662 GN=LOC111451824 PE=3 SV=1)

HSP 1 Score: 658 bits (1698), Expect = 5.05e-233
Identity = 323/395 (81.77%), Postives = 353/395 (89.37%), Query Frame = 0


Query: 27  LQYIEQVTAHADQVQRRVLTEILSANAHVEYLRH-GLDGYMDTKTFKKTMPVVSYDDIKP 86
           LQ+IE++T HADQVQRRVLTEILS NAHVEYL+  GLDG MD   FK  +PVVSY+++KP
Sbjct: 10  LQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVPVVSYENLKP 69

Query: 87  YVDRIANGDPSPILCSKPISEFLTSSGTSGGERKLMPTTEEELERKSLLYSLLMPVMNQF 146
           Y+DRIANGD SPILCS+P+SEFLTSSGTSGGERKLMPTTEEEL+R+S LYSLLMPVMNQF
Sbjct: 70  YIDRIANGDASPILCSEPVSEFLTSSGTSGGERKLMPTTEEELKRRSFLYSLLMPVMNQF 129

Query: 147 IPGLDKGKGMYFLFVKSESTTPGGLLARPVLTSYYRSPHFKDRPYDPYNQYTSPNETILC 206
           IPGL+KGKGMYFLFVKSESTTPGGL ARPVLTSYY+S HFKDRPYD Y  YTSPN+TILC
Sbjct: 130 IPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSPNQTILC 189

Query: 207 TDSYESMYSQFLCGLCLSREVLRVGAVFASGFIRAIKFLETHWLLLSNDIRTGTINPRIT 266
           TDSY+SMY QFLCGLCLS++VLRVG+VFASGFIRAIKFLETHW+LL+NDIRTGTINPRI 
Sbjct: 190 TDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIRTGTINPRIR 249

Query: 267 DPLVRQSVLNILKPDPELADLIESECSKGTWEGIISRLWRNTKYIDVIVTGTMSQYIPTL 326
           DPLVRQSV+NILKPDP LADLIE ECSKGTW GII RLW NTKYIDVIVTGTMSQYIPTL
Sbjct: 250 DPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIIPRLWPNTKYIDVIVTGTMSQYIPTL 309

Query: 327 DYYSNGLPLVCTMYASSECYFGINLNPLCNPSEVSYTLIPTMAYFEFLPLDGTNGFGYSS 386
           DYYSNGLPLVCTMYASSECYFG+NLNPLCNP+EVSYTLIPTMAYFEFLPLD         
Sbjct: 310 DYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLD--------- 369

Query: 387 TCQSEANQKTDQELVDLVDVQLGKEYELVITTHAG 420
                  +K  +ELVDLVDV+LGKEYELVITT++G
Sbjct: 370 -------RKMSEELVDLVDVELGKEYELVITTYSG 388

[Close]

BLAST of MC02g_new0101 vs. ExPASy TrEMBL
Match: A0A6J1I4E6 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita maxima OX=3661 GN=LOC111469778 PE=3 SV=1)

HSP 1 Score: 656 bits (1693), Expect = 2.91e-232
Identity = 321/395 (81.27%), Postives = 355/395 (89.87%), Query Frame = 0


Query: 27  LQYIEQVTAHADQVQRRVLTEILSANAHVEYLRH-GLDGYMDTKTFKKTMPVVSYDDIKP 86
           LQ+IE++T HADQVQRRVLTEILS NAHVEYL+  GLDG MD   FK  +PVVSY+++KP
Sbjct: 10  LQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVPVVSYENLKP 69

Query: 87  YVDRIANGDPSPILCSKPISEFLTSSGTSGGERKLMPTTEEELERKSLLYSLLMPVMNQF 146
           Y+DRIANGD SPILCS+PISEFLTSSGTSGGERKLMPTT+EEL+R+S LYSLLMPVMNQF
Sbjct: 70  YIDRIANGDASPILCSEPISEFLTSSGTSGGERKLMPTTKEELKRRSFLYSLLMPVMNQF 129

Query: 147 IPGLDKGKGMYFLFVKSESTTPGGLLARPVLTSYYRSPHFKDRPYDPYNQYTSPNETILC 206
           IPGL+KGKGMYFLFVKSESTTPGGL ARPVLTSYY+S HFKDRPYD Y  YTSPN+TILC
Sbjct: 130 IPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSPNQTILC 189

Query: 207 TDSYESMYSQFLCGLCLSREVLRVGAVFASGFIRAIKFLETHWLLLSNDIRTGTINPRIT 266
           TDSY+SMYSQFLCGLCLS++VLRVG+VFASGFIRAIKFLETHW+LL+NDIRTGTINPRI+
Sbjct: 190 TDSYQSMYSQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIRTGTINPRIS 249

Query: 267 DPLVRQSVLNILKPDPELADLIESECSKGTWEGIISRLWRNTKYIDVIVTGTMSQYIPTL 326
           DPLVRQSV+NILKPDP LADLIE ECSKGTW GII+RLW NTKY+DVIVTGTMSQYIPTL
Sbjct: 250 DPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIITRLWPNTKYVDVIVTGTMSQYIPTL 309

Query: 327 DYYSNGLPLVCTMYASSECYFGINLNPLCNPSEVSYTLIPTMAYFEFLPLDGTNGFGYSS 386
           D YSNGLPLVCTMYASSECYFG+NLNPLCNP+EVSYTLIPTMAYFEFLPLD         
Sbjct: 310 DKYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLD--------- 369

Query: 387 TCQSEANQKTDQELVDLVDVQLGKEYELVITTHAG 420
                  +K  +ELVDLVDV+LGKEYEL+ITT++G
Sbjct: 370 -------RKMSEELVDLVDVELGKEYELIITTYSG 388

[Close]

BLAST of MC02g_new0101 vs. ExPASy TrEMBL
Match: A0A6A3AIU7 (Indole-3-acetic acid-amido synthetase GH3.6 OS=Hibiscus syriacus OX=106335 GN=F3Y22_tig00110472pilonHSYRG00040 PE=3 SV=1)

HSP 1 Score: 644 bits (1661), Expect = 5.62e-227
Identity = 319/422 (75.59%), Postives = 359/422 (85.07%), Query Frame = 0


Query: 1   MPEASN-NLPPEPDSLIGSSHEKHERALQYIEQVTAHADQVQRRVLTEILSANAHVEYL- 60
           MPEA N  L P   SL      K+++ LQ+IE VT+  D VQR+VL EIL  NAHVEYL 
Sbjct: 1   MPEAPNITLKPTDYSL----ELKNKKTLQFIEDVTSSPDGVQRKVLEEILERNAHVEYLQ 60

Query: 61  RHGLDGYMDTKTFKKTMPVVSYDDIKPYVDRIANGDPSPILCSKPISEFLTSSGTSGGER 120
           +HGL+G+ D  +FKK MPV++Y+DI+PYV+RIANGD SPILCSKPISEFLTSSGTSGGER
Sbjct: 61  KHGLNGHTDRGSFKKIMPVITYEDIQPYVERIANGDTSPILCSKPISEFLTSSGTSGGER 120

Query: 121 KLMPTTEEELERKSLLYSLLMPVMNQFIPGLDKGKGMYFLFVKSESTTPGGLLARPVLTS 180
           KLMPT EEEL R+SLLYSLLMPVM+QF+PGLDKGKGMYFLF+KSE+ TPGGL ARPVLTS
Sbjct: 121 KLMPTIEEELGRRSLLYSLLMPVMSQFVPGLDKGKGMYFLFIKSEAKTPGGLAARPVLTS 180

Query: 181 YYRSPHFKDRPYDPYNQYTSPNETILCTDSYESMYSQFLCGLCLSREVLRVGAVFASGFI 240
           YY+S HFKDRPYDPY  YTSPNETILC DSY+SMYSQ LCGLC  +EVLRVGAVFASGFI
Sbjct: 181 YYKSYHFKDRPYDPYTNYTSPNETILCPDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFI 240

Query: 241 RAIKFLETHWLLLSNDIRTGTINPRITDPLVRQSVLNILKPDPELADLIESECSKGTWEG 300
           RAIKFLE HW LL NDIRTGTI  +ITD  VR++V+NILKPDPELAD IE+ECSK +W+G
Sbjct: 241 RAIKFLEKHWRLLCNDIRTGTIGSQITDQSVREAVMNILKPDPELADFIEAECSKDSWQG 300

Query: 301 IISRLWRNTKYIDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGINLNPLCNPSE 360
           II+RLW NTKY+DVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFG+NLNPLC PSE
Sbjct: 301 IITRLWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSE 360

Query: 361 VSYTLIPTMAYFEFLPLDGTNGFGYSSTCQSEANQKTDQELVDLVDVQLGKEYELVITTH 420
           VSYTLIPTMAYFEFLP+   NG   S +     N+K  QELVDLVDV+LGKEYELV+TT+
Sbjct: 361 VSYTLIPTMAYFEFLPIQRNNGVSNSISVPKTLNEKEQQELVDLVDVKLGKEYELVVTTY 418

[Close]

BLAST of MC02g_new0101 vs. TAIR 10
Match: AT5G54510.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 614.8 bits (1584), Expect = 5.3e-176
Identity = 299/401 (74.56%), Postives = 342/401 (85.29%), Query Frame = 0


Query: 21  EKHERALQYIEQVTAHADQVQRRVLTEILSANAHVEYL-RHGLDGYMDTKTFKKTMPVVS 80
           EK++  LQ+IE VT +AD VQRRVL EILS NA VEYL RHGL+G  D +TFK  MPVV+
Sbjct: 19  EKNKNKLQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHGLEGRTDRETFKHIMPVVT 78

Query: 81  YDDIKPYVDRIANGDPSPILCSKPISEFLTSSGTSGGERKLMPTTEEELERKSLLYSLLM 140
           Y+DI+P ++RIANGD S +LCS PISEFLTSSGTSGGERKLMPT EEEL+R+SLLYSLLM
Sbjct: 79  YEDIQPEINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSLLM 138

Query: 141 PVMNQFIPGLDKGKGMYFLFVKSESTTPGGLLARPVLTSYYRSPHFKDRPYDPYNQYTSP 200
           PVM+QF+PGLDKGKGMYFLF+KSES TPGGL ARPVLTSYY+S HFK+RPYDPY  YTSP
Sbjct: 139 PVMDQFVPGLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKNRPYDPYTNYTSP 198

Query: 201 NETILCTDSYESMYSQFLCGLCLSREVLRVGAVFASGFIRAIKFLETHWLLLSNDIRTGT 260
           N+TILC+DSY+SMYSQ LCGLC  +EVLRVGAVFASGFIRAIKFLE HW  L+ DIRTGT
Sbjct: 199 NQTILCSDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIKFLEKHWPELARDIRTGT 258

Query: 261 INPRITDPLVRQSVLNILKPDPELADLIESECSKGTWEGIISRLWRNTKYIDVIVTGTMS 320
           ++  ITD  VR++V  ILKPDP+LAD +ESEC K +W+GII+RLW NTKY+DVIVTGTMS
Sbjct: 259 LSSEITDSSVREAVGEILKPDPKLADFVESECRKTSWQGIITRLWPNTKYVDVIVTGTMS 318

Query: 321 QYIPTLDYYSNGLPLVCTMYASSECYFGINLNPLCNPSEVSYTLIPTMAYFEFLPLDGTN 380
           QYIPTLDYYSNGLPLVCTMYASSECYFG+NL PLC PSEVSYTLIP MAYFEFLP+   +
Sbjct: 319 QYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPNMAYFEFLPVHRNS 378

Query: 381 GFGYSSTCQSEANQKTDQELVDLVDVQLGKEYELVITTHAG 421
           G   S +      +K  QELVDLVDV+LG+EYELV+TT+AG
Sbjct: 379 GVTSSISLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAG 419

[Close]

BLAST of MC02g_new0101 vs. TAIR 10
Match: AT4G27260.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 609.8 bits (1571), Expect = 1.7e-174
Identity = 301/421 (71.50%), Postives = 350/421 (83.14%), Query Frame = 0


Query: 1   MPEASNNLPPEPDSLIGSSHEKHERALQYIEQVTAHADQVQRRVLTEILSANAHVEYL-R 60
           MPEA      E   L  +  +K+++ LQ IE++T++ADQVQR+VL EIL+ NA VEYL R
Sbjct: 1   MPEAPKKESLEVFDL--TLDQKNKQKLQLIEELTSNADQVQRQVLEEILTRNADVEYLRR 60

Query: 61  HGLDGYMDTKTFKKTMPVVSYDDIKPYVDRIANGDPSPILCSKPISEFLTSSGTSGGERK 120
           H L+G  D +TFK  MPV++Y+DI+P ++RIANGD SPIL SKPISEFLTSSGTSGGERK
Sbjct: 61  HDLNGRTDRETFKNIMPVITYEDIEPEINRIANGDKSPILSSKPISEFLTSSGTSGGERK 120

Query: 121 LMPTTEEELERKSLLYSLLMPVMNQFIPGLDKGKGMYFLFVKSESTTPGGLLARPVLTSY 180
           LMPT EEEL+R+SLLYSLLMPVM+QF+PGL+ GKGMYFLF+KSES TPGGL ARPVLTSY
Sbjct: 121 LMPTIEEELDRRSLLYSLLMPVMSQFVPGLENGKGMYFLFIKSESKTPGGLPARPVLTSY 180

Query: 181 YRSPHFKDRPYDPYNQYTSPNETILCTDSYESMYSQFLCGLCLSREVLRVGAVFASGFIR 240
           Y+S HFK+RPYDPY  YTSPNETILC+DSY+SMYSQ LCGLC  +EVLRVGAVFASGFIR
Sbjct: 181 YKSSHFKERPYDPYTNYTSPNETILCSDSYQSMYSQMLCGLCQHQEVLRVGAVFASGFIR 240

Query: 241 AIKFLETHWLLLSNDIRTGTINPRITDPLVRQSVLNILKPDPELADLIESECSKGTWEGI 300
           AIKFLE HW+ L  DIRTGT++  ITDP VR++V  ILKP P+LAD +E EC K +W+GI
Sbjct: 241 AIKFLEKHWIELVRDIRTGTLSSLITDPSVREAVAKILKPSPKLADFVEFECKKSSWQGI 300

Query: 301 ISRLWRNTKYIDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGINLNPLCNPSEV 360
           I+RLW NTKY+DVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFG+NL PLC PSEV
Sbjct: 301 ITRLWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEV 360

Query: 361 SYTLIPTMAYFEFLPLDGTNGFGYSSTCQSEANQKTDQELVDLVDVQLGKEYELVITTHA 420
           SYTLIP+MAYFEFLP+   NG   S        +K  QELVDLVDV+LG+EYELV+TT+A
Sbjct: 361 SYTLIPSMAYFEFLPVHRNNGVTNSINLPKALTEKEQQELVDLVDVKLGQEYELVVTTYA 419

[Close]

BLAST of MC02g_new0101 vs. TAIR 10
Match: AT2G14960.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 520.4 bits (1339), Expect = 1.4e-147
Identity = 253/401 (63.09%), Postives = 307/401 (76.56%), Query Frame = 0


Query: 21  EKHERALQYIEQVTAHADQVQRRVLTEILSANAHVEYLRH-GLDGYMDTKTFKKTMPVVS 80
           EK  +AL++IE++T +AD VQ  +L EIL+ NA  EYLR   L G  D  TFK  +PV++
Sbjct: 17  EKDAKALRFIEEMTRNADTVQENLLAEILARNADTEYLRRFNLCGATDRDTFKTKIPVIT 76

Query: 81  YDDIKPYVDRIANGDPSPILCSKPISEFLTSSGTSGGERKLMPTTEEELERKSLLYSLLM 140
           Y+D++P + RIA+GD SPIL + PISEFLTSSGTS GERKLMPT +EEL+R+ LLYSLLM
Sbjct: 77  YEDLQPEIQRIADGDRSPILSAHPISEFLTSSGTSAGERKLMPTIKEELDRRQLLYSLLM 136

Query: 141 PVMNQFIPGLDKGKGMYFLFVKSESTTPGGLLARPVLTSYYRSPHFKDRPYDPYNQYTSP 200
           PVMN ++PGLDKGKGMYFLFVKSE+ TPGGL ARPVLTSYY+S HF+ RPYDPYN YTSP
Sbjct: 137 PVMNLYVPGLDKGKGMYFLFVKSETKTPGGLPARPVLTSYYKSEHFRSRPYDPYNVYTSP 196

Query: 201 NETILCTDSYESMYSQFLCGLCLSREVLRVGAVFASGFIRAIKFLETHWLLLSNDIRTGT 260
           NE ILC DS++SMY+Q LCGL     VLRVGAVFASG +RAI+FL+ HW   ++DI  G 
Sbjct: 197 NEAILCPDSFQSMYTQMLCGLLDRLSVLRVGAVFASGLLRAIRFLQLHWSRFAHDIELGC 256

Query: 261 INPRITDPLVRQSVLNILKPDPELADLIESECSKGTWEGIISRLWRNTKYIDVIVTGTMS 320
           ++  ITDP +RQ +  ILKPDP LA+ I  EC    WE II+R+W NTKY+DVIVTG M+
Sbjct: 257 LDSEITDPSIRQCMSGILKPDPVLAEFIRRECKSDNWEKIITRIWPNTKYLDVIVTGAMA 316

Query: 321 QYIPTLDYYSNGLPLVCTMYASSECYFGINLNPLCNPSEVSYTLIPTMAYFEFLPLDGTN 380
           QYIPTL+YYS GLP+ CTMYASSECYFG+NLNP+  PSEVSYT++P MAYFEF+PL GT 
Sbjct: 317 QYIPTLEYYSGGLPMACTMYASSECYFGLNLNPMSKPSEVSYTIMPNMAYFEFIPLGGT- 376

Query: 381 GFGYSSTCQSEANQKTDQELVDLVDVQLGKEYELVITTHAG 421
                             + V+LVDV +GKEYELV+TT+AG
Sbjct: 377 ------------------KAVELVDVNIGKEYELVVTTYAG 398

[Close]

BLAST of MC02g_new0101 vs. TAIR 10
Match: AT4G37390.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 518.1 bits (1333), Expect = 6.8e-147
Identity = 257/414 (62.08%), Postives = 321/414 (77.54%), Query Frame = 0


Query: 9   PPEPDSLIGSSHEKHERALQYIEQVTAHADQVQRRVLTEILSANAHVEYL-RHGLDGYMD 68
           P +   +  ++ EK  +AL++IE++T + D VQ +VL EIL+ N++ EYL R  LDG +D
Sbjct: 6   PLQSRMVSATTSEKDVKALKFIEEMTRNPDSVQEKVLGEILTRNSNTEYLKRFDLDGVVD 65

Query: 69  TKTFKKTMPVVSYDDIKPYVDRIANGDPSPILCSKPISEFLTSSGTSGGERKLMPTTEEE 128
            KTFK  +PVV+Y+D+KP + RI+NGD SPIL S PI+EFLTSSGTS GERKLMPT EE+
Sbjct: 66  RKTFKSKVPVVTYEDLKPEIQRISNGDCSPILSSHPITEFLTSSGTSAGERKLMPTIEED 125

Query: 129 LERKSLLYSLLMPVMNQFIPGLDKGKGMYFLFVKSESTTPGGLLARPVLTSYYRSPHFKD 188
           L+R+ LLYSLLMPVMN ++PGLDKGKG+YFLFVKSES T GGL ARPVLTSYY+S HFK 
Sbjct: 126 LDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSESKTSGGLPARPVLTSYYKSDHFKR 185

Query: 189 RPYDPYNQYTSPNETILCTDSYESMYSQFLCGLCLSREVLRVGAVFASGFIRAIKFLETH 248
           RPYDPYN YTSPNE ILC+DS +SMY+Q LCGL +  EVLR+GAVFASG +RAI FL+ +
Sbjct: 186 RPYDPYNVYTSPNEAILCSDSSQSMYAQMLCGLLMRHEVLRLGAVFASGLLRAISFLQNN 245

Query: 249 WLLLSNDIRTGTINPRITDPLVRQSVLNIL-KPDPELADLIESECSKGTWEGIISRLWRN 308
           W  L+ DI TGT++ RI DP ++  +  IL KPD ELA+ +   CS+  WEGII+++W N
Sbjct: 246 WKELARDISTGTLSSRIFDPAIKNRMSKILTKPDQELAEFLVGVCSQENWEGIITKIWPN 305

Query: 309 TKYIDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGINLNPLCNPSEVSYTLIPT 368
           TKY+DVIVTG M+QYIPTL+YYS GLP+ CTMYASSE YFGINL P+C PSEVSYT++P 
Sbjct: 306 TKYLDVIVTGAMAQYIPTLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSYTIMPN 365

Query: 369 MAYFEFLPLDGTNGFGYSSTCQSEANQKTDQELVDLVDVQLGKEYELVITTHAG 421
           MAYFEFLP    +G G +     E +      LV+L +V++GKEYELVITT+AG
Sbjct: 366 MAYFEFLP-HNHDGDGAAEASLDETS------LVELANVEVGKEYELVITTYAG 412

[Close]

BLAST of MC02g_new0101 vs. TAIR 10
Match: AT2G23170.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 506.5 bits (1303), Expect = 2.0e-143
Identity = 254/409 (62.10%), Postives = 313/409 (76.53%), Query Frame = 0


Query: 15  LIGSSHEKHERALQYIEQVTAHADQVQRRVLTEILSANAHVEYL-RHGLDGYMDTKTFKK 74
           ++ S   K  +AL++IE++T + D VQ++V+ EILS N+  EYL R GL G+ D KTFK 
Sbjct: 11  MMHSPSTKDVKALRFIEEMTRNVDFVQKKVIREILSRNSDTEYLKRFGLKGFTDRKTFKT 70

Query: 75  TMPVVSYDDIKPYVDRIANGDPSPILCSKPISEFLTSSGTSGGERKLMPTTEEELERKSL 134
            +PVV YDD+KP + RIANGD S IL S PI+EFLTSSGTS GERKLMPT +E+++R+ L
Sbjct: 71  KVPVVIYDDLKPEIQRIANGDRSMILSSYPITEFLTSSGTSAGERKLMPTIDEDMDRRQL 130

Query: 135 LYSLLMPVMNQFIPGLDKGKGMYFLFVKSESTTPGGLLARPVLTSYYRSPHFKDRPYDPY 194
           LYSLLMPVMN ++PGLDKGK +YFLFVK+ES TPGGL ARPVLTSYY+S  FK RP DPY
Sbjct: 131 LYSLLMPVMNLYVPGLDKGKALYFLFVKTESKTPGGLPARPVLTSYYKSEQFKRRPNDPY 190

Query: 195 NQYTSPNETILCTDSYESMYSQFLCGLCLSREVLRVGAVFASGFIRAIKFLETHWLLLSN 254
           N YTSPNE ILC DS +SMY+Q LCGL +  EVLR+GAVFASG +RAI FL+T+W  L++
Sbjct: 191 NVYTSPNEAILCPDSSQSMYTQMLCGLLMRHEVLRLGAVFASGLLRAIGFLQTNWKELAD 250

Query: 255 DIRTGTINPRITDPLVRQSVLNIL-KPDPELADLIESECSK-GTWEGIISRLWRNTKYID 314
           DI TGT++ RI+DP +++S+  IL KPD ELAD I S C +  +WEGII+++W NTKY+D
Sbjct: 251 DISTGTLSSRISDPAIKESMSKILTKPDQELADFITSVCGQDNSWEGIITKIWPNTKYLD 310

Query: 315 VIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGINLNPLCNPSEVSYTLIPTMAYFE 374
           VIVTG M+QYIP L+YYS GLP+ CTMYASSE YFGINL P+C PSEVSYT++P MAYFE
Sbjct: 311 VIVTGAMAQYIPMLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSYTIMPNMAYFE 370

Query: 375 FLPLDGTNGFGYSSTCQSEANQKTDQELVDLVDVQLGKEYELVITTHAG 421
           FLP                       ELV+L DV++GKEYELVITT+AG
Sbjct: 371 FLP--------------HHEVPTEKSELVELADVEVGKEYELVITTYAG 405

The following BLAST results are available for this feature:

BLAST of MC02g_new0101 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LSQ4	7.5e-175	74.56	Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana OX=3702 GN=G...	[more]
O81829	2.4e-173	71.50	Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana OX=3702 GN=G...	[more]
A3BLS0	6.4e-150	62.72	Indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. indica OX=399...	[more]
Q0D4Z6	6.4e-150	62.72	Indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. japonica OX=3...	[more]
Q8LQM5	4.6e-148	64.75	Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Oryza sativa subsp. japo...	[more]

BLAST of MC02g_new0101 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022140599.1	8.30e-309	99.52	indole-3-acetic acid-amido synthetase GH3.6-like isoform X2 [Momordica charantia...	[more]
XP_022140598.1	2.70e-236	99.70	indole-3-acetic acid-amido synthetase GH3.6-like isoform X1 [Momordica charantia...	[more]
KAG7034947.1	3.27e-233	81.77	Indole-3-acetic acid-amido synthetase GH3.6, partial [Cucurbita argyrosperma sub...	[more]
XP_022948165.1	1.04e-232	81.77	indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita moschata]	[more]
XP_023533206.1	1.34e-232	81.52	indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of MC02g_new0101 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CG47	4.02e-309	99.52	indole-3-acetic acid-amido synthetase GH3.6-like isoform X2 OS=Momordica charant...	[more]
A0A6J1CIC6	1.31e-236	99.70	indole-3-acetic acid-amido synthetase GH3.6-like isoform X1 OS=Momordica charant...	[more]
A0A6J1G8Z2	5.05e-233	81.77	indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita moschata OX=3662 G...	[more]
A0A6J1I4E6	2.91e-232	81.27	indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita maxima OX=3661 GN=...	[more]
A0A6A3AIU7	5.62e-227	75.59	Indole-3-acetic acid-amido synthetase GH3.6 OS=Hibiscus syriacus OX=106335 GN=F3...	[more]

BLAST of MC02g_new0101 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G54510.1	5.3e-176	74.56	Auxin-responsive GH3 family protein	[more]
AT4G27260.1	1.7e-174	71.50	Auxin-responsive GH3 family protein	[more]
AT2G14960.1	1.4e-147	63.09	Auxin-responsive GH3 family protein	[more]
AT4G37390.1	6.8e-147	62.08	Auxin-responsive GH3 family protein	[more]
AT2G23170.1	2.0e-143	62.10	Auxin-responsive GH3 family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004993	GH3 family	PFAM	PF03321	GH3	coord: 30..420 e-value: 3.3E-132 score: 442.1
IPR004993	GH3 family	PANTHER	PTHR31901	GH3 DOMAIN-CONTAINING PROTEIN	coord: 15..422
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..22
None	No IPR available	PANTHER	PTHR31901:SF70	BNAC03G14570D PROTEIN	coord: 15..422

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC02g_new0101.1	MC02g_new0101.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0010279	indole-3-acetic acid amido synthetase activity