MC02g_new0100 (gene) Bitter gourd (Dali-11) v1

BLAST of MC02g_new0100 vs. ExPASy Swiss-Prot
Match: Q9LSQ4 (Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana OX=3702 GN=GH3.6 PE=1 SV=1)

HSP 1 Score: 714.9 bits (1844), Expect = 7.5e-205
Identity = 352/592 (59.46%), Postives = 446/592 (75.34%), Query Frame = 0

Query: 6   DTTLDEKDKQVLQHLEDVTSNADEIQRRVLTEILSKNAHVEYLQQHGLDGSTDASTFKNL 65
           D +L EK+K  LQ +EDVT+NAD++QRRVL EILS+NA VEYL++HGL+G TD  TFK++
Sbjct: 14  DESLAEKNKNKLQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHGLEGRTDRETFKHI 73

Query: 66  VPIVSYEHLKPYIDRIANGDDSPILCAHPITEFLISSGTSGGEQKLFPMFEQELVRRLSI 125
           +P+V+YE ++P I+RIANGD S +LC++PI+EFL SSGTSGGE+KL P  E+EL RR  +
Sbjct: 74  MPVVTYEDIQPEINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLMPTIEEELDRRSLL 133

Query: 126 RNCMRPKIKELFPSLYNGKEMFFHFARAESVTKGGIVARSASTSLSRSSDFNNMESG--- 185
            + + P + +  P L  GK M+F F ++ES T GG+ AR   TS  +SS F N       
Sbjct: 134 YSLLMPVMDQFVPGLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKNRPYDPYT 193

Query: 186 SYTSPNEIIRCTDAYQSLYCQLLCGLLQREKVVGIGALFASTLVYVSKFLENHWVLLAND 245
           +YTSPN+ I C+D+YQS+Y Q+LCGL Q ++V+ +GA+FAS  +   KFLE HW  LA D
Sbjct: 194 NYTSPNQTILCSDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIKFLEKHWPELARD 253

Query: 246 IRTGTVNTKITDQLVRESVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAIV 305
           IRTGT++++ITD  VRE+V  ILKPDP+LADF+ESEC K +W+GI+TRLWPNTK+++ IV
Sbjct: 254 IRTGTLSSEITDSSVREAVGEILKPDPKLADFVESECRKTSWQGIITRLWPNTKYVDVIV 313

Query: 306 TGSMSQYIPQLNYYSNCLPLISLFYASSECYFGINLDPLSKPEEISYTFIPTMAYFEFLP 365
           TG+MSQYIP L+YYSN LPL+   YASSECYFG+NL PL KP E+SYT IP MAYFEFLP
Sbjct: 314 TGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPNMAYFEFLP 373

Query: 366 VDRANA-----------NREVGQELVDLVDVKLGQEYEVIVTTYTGLYRYCIGDIVRVTG 425
           V R +              +  QELVDLVDVKLGQEYE++VTTY GLYRY +GD++ V G
Sbjct: 374 VHRNSGVTSSISLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLYRYRVGDVLSVAG 433

Query: 426 FKYKAPCFSFVCRKNVVLSLDFEKTDEAELHNAVKGAGELLNPFGATIADYTSYADNSTF 485
           FK  AP FSF+CRKNVVLS+D +KTDE EL NAVK A   L PF A++++YTSYAD S+ 
Sbjct: 434 FKNNAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSI 493

Query: 486 PGHYVLYWEIHFKEEISIIPSSIYEDCCLAVEESLNSLYRQARSHQKVINPLEIKMVEAG 545
           PGHYVL+WE+      + IP S++EDCCL +EESLNS+YRQ R   K I PLEIKMVE+G
Sbjct: 494 PGHYVLFWELCLNGN-TPIPPSVFEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKMVESG 553

Query: 546 TFDKLLERATYQGASISQYKTPRCVTSTQIVELLDTKVVASYFSKKYPKWDP 584
           TFDKL++ A   GASI+QYKTPRCV    I+ELL+++VV SYFS K PKW P
Sbjct: 554 TFDKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWSP 604

BLAST of MC02g_new0100 vs. ExPASy Swiss-Prot
Match: O81829 (Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana OX=3702 GN=GH3.5 PE=1 SV=1)

HSP 1 Score: 710.7 bits (1833), Expect = 1.4e-203
Identity = 351/592 (59.29%), Postives = 446/592 (75.34%), Query Frame = 0

Query: 6   DTTLDEKDKQVLQHLEDVTSNADEIQRRVLTEILSKNAHVEYLQQHGLDGSTDASTFKNL 65
           D TLD+K+KQ LQ +E++TSNAD++QR+VL EIL++NA VEYL++H L+G TD  TFKN+
Sbjct: 14  DLTLDQKNKQKLQLIEELTSNADQVQRQVLEEILTRNADVEYLRRHDLNGRTDRETFKNI 73

Query: 66  VPIVSYEHLKPYIDRIANGDDSPILCAHPITEFLISSGTSGGEQKLFPMFEQELVRRLSI 125
           +P+++YE ++P I+RIANGD SPIL + PI+EFL SSGTSGGE+KL P  E+EL RR  +
Sbjct: 74  MPVITYEDIEPEINRIANGDKSPILSSKPISEFLTSSGTSGGERKLMPTIEEELDRRSLL 133

Query: 126 RNCMRPKIKELFPSLYNGKEMFFHFARAESVTKGGIVARSASTSLSRSSDFNNMESG--- 185
            + + P + +  P L NGK M+F F ++ES T GG+ AR   TS  +SS F         
Sbjct: 134 YSLLMPVMSQFVPGLENGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKERPYDPYT 193

Query: 186 SYTSPNEIIRCTDAYQSLYCQLLCGLLQREKVVGIGALFASTLVYVSKFLENHWVLLAND 245
           +YTSPNE I C+D+YQS+Y Q+LCGL Q ++V+ +GA+FAS  +   KFLE HW+ L  D
Sbjct: 194 NYTSPNETILCSDSYQSMYSQMLCGLCQHQEVLRVGAVFASGFIRAIKFLEKHWIELVRD 253

Query: 246 IRTGTVNTKITDQLVRESVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAIV 305
           IRTGT+++ ITD  VRE+V  ILKP P+LADF+E EC K +W+GI+TRLWPNTK+++ IV
Sbjct: 254 IRTGTLSSLITDPSVREAVAKILKPSPKLADFVEFECKKSSWQGIITRLWPNTKYVDVIV 313

Query: 306 TGSMSQYIPQLNYYSNCLPLISLFYASSECYFGINLDPLSKPEEISYTFIPTMAYFEFLP 365
           TG+MSQYIP L+YYSN LPL+   YASSECYFG+NL PL KP E+SYT IP+MAYFEFLP
Sbjct: 314 TGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPSMAYFEFLP 373

Query: 366 VDRANA-----------NREVGQELVDLVDVKLGQEYEVIVTTYTGLYRYCIGDIVRVTG 425
           V R N              +  QELVDLVDVKLGQEYE++VTTY GL RY +GD++RVTG
Sbjct: 374 VHRNNGVTNSINLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLCRYRVGDLLRVTG 433

Query: 426 FKYKAPCFSFVCRKNVVLSLDFEKTDEAELHNAVKGAGELLNPFGATIADYTSYADNSTF 485
           FK KAP FSF+CRKNVVLS+D +KTDE EL NAVK A   L PF A++++YTSYAD S+ 
Sbjct: 434 FKNKAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSI 493

Query: 486 PGHYVLYWEIHFKEEISIIPSSIYEDCCLAVEESLNSLYRQARSHQKVINPLEIKMVEAG 545
           PGHYVL+WE+   +  + IP S++EDCCLAVEES N++YRQ R   K I PLEIK+VE G
Sbjct: 494 PGHYVLFWELCL-DGNTPIPPSVFEDCCLAVEESFNTVYRQGRVSDKSIGPLEIKIVEPG 553

Query: 546 TFDKLLERATYQGASISQYKTPRCVTSTQIVELLDTKVVASYFSKKYPKWDP 584
           TFDKL++ A   GASI+QYKTPRCV    I+ELL+++VV SYFS K PKW P
Sbjct: 554 TFDKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWVP 604

BLAST of MC02g_new0100 vs. ExPASy Swiss-Prot
Match: Q8LQM5 (Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.1 PE=2 SV=1)

HSP 1 Score: 639.4 bits (1648), Expect = 4.0e-182
Identity = 315/574 (54.88%), Postives = 415/574 (72.30%), Query Frame = 0

Query: 15  QVLQHLEDVTSNADEIQRRVLTEILSKNAHVEYLQQHGLDGSTD-ASTFKNLVPIVSYEH 74
           + L+ +E VT+NA ++QRRVL EIL++NA  EYL+++G+ GS D    F+ LVP+V+YE 
Sbjct: 22  EALEFIEHVTANAGQVQRRVLGEILAQNAPAEYLRRYGIPGSPDVVDAFRRLVPLVTYEG 81

Query: 75  LKPYIDRIANGDDSPILCAHPITEFLISSGTSGGEQKLFPMFEQELVRRLSIRNCMRPKI 134
           L+P I RIANGD SPI    PI+EFL SSGTSGGE+KL P    E+ RR  + + + P +
Sbjct: 82  LQPDILRIANGDTSPIFSGKPISEFLTSSGTSGGERKLMPTIADEMNRRSLLYSLLMPVM 141

Query: 135 KELFPSLYNGKEMFFHFARAESVTKGGIVARSASTSLSRSSDFNNMESG---SYTSPNEI 194
            +    L  GK M+  F +AES T GG+ AR   TS  RS  F +       SYTSP+E 
Sbjct: 142 SQSVSGLDKGKAMYLLFVKAESRTPGGLAARPVLTSYYRSRQFLDRPRDPYTSYTSPDEA 201

Query: 195 IRCTDAYQSLYCQLLCGLLQREKVVGIGALFASTLVYVSKFLENHWVLLANDIRTGTVNT 254
           I C D+YQS+Y QLLCGL+ R  V+ +GA+FAS  +    FLE HW  L +DIRTG ++ 
Sbjct: 202 ILCVDSYQSMYAQLLCGLVHRADVLRVGAVFASGFLRAIHFLEKHWARLCHDIRTGELDP 261

Query: 255 KITDQLVRESVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAIVTGSMSQYI 314
           +ITD++VR++V  +L+ DP LAD IE EC++ +WEGI+ RLWP TK+I+ IVTG+MSQYI
Sbjct: 262 EITDRVVRDAVGRVLRADPALADAIEDECARASWEGIIRRLWPRTKYIDVIVTGTMSQYI 321

Query: 315 PQLNYYSNCLPLISLFYASSECYFGINLDPLSKPEEISYTFIPTMAYFEFLPVDRANANR 374
           P L +Y   LPL    YASSECYFG+NL+P+ KP +++YT IPTM Y+EFLPV+  NA  
Sbjct: 322 PTLEFYGGGLPLTCTMYASSECYFGLNLNPMCKPSDVAYTLIPTMCYYEFLPVNCNNATA 381

Query: 375 EVG-QELVDLVDVKLGQEYEVIVTTYTGLYRYCIGDIVRVTGFKYKAPCFSFVCRKNVVL 434
           E   ++LVDLVDVKLG EYE++VTTY+GLYRY +GD++RV GFK KAP FSFV R+NV L
Sbjct: 382 EASHRDLVDLVDVKLGHEYELVVTTYSGLYRYRVGDVLRVAGFKNKAPMFSFVRRQNVAL 441

Query: 435 SLDFEKTDEAELHNAVKGAGELLNPFGATIADYTSYADNSTFPGHYVLYWEIHFKEEISI 494
           S+D +KTDE ELH AV GA + L PFGA++ +YTSYAD +T PGHYVL+WE+  +   + 
Sbjct: 442 SVDSDKTDETELHAAVSGAVQHLAPFGASLVEYTSYADAATIPGHYVLFWEL--RAGSTA 501

Query: 495 IPSSIYEDCCLAVEESLNSLYRQARSHQKVINPLEIKMVEAGTFDKLLERATYQGASISQ 554
           +P+S++E+CCL+VEE+LNS+YRQ R+  + I PLEI++V  GTFDKL++ A  +GASI+Q
Sbjct: 502 VPASVFEECCLSVEEALNSVYRQGRACDRSIGPLEIRVVAEGTFDKLMDYAISRGASINQ 561

Query: 555 YKTPRCVTSTQIVELLDTKVVASYFSKKYPKWDP 584
           YK PRCV    +VELLD +V   YFS K PKW P
Sbjct: 562 YKAPRCVRPGPVVELLDARVQGKYFSPKCPKWSP 593

BLAST of MC02g_new0100 vs. ExPASy Swiss-Prot
Match: Q60EJ6 (Probable indole-3-acetic acid-amido synthetase GH3.4 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.4 PE=2 SV=1)

HSP 1 Score: 607.4 bits (1565), Expect = 1.7e-172
Identity = 309/590 (52.37%), Postives = 410/590 (69.49%), Query Frame = 0

Query: 4   ALDTTLDEKDKQVLQHLEDVTSNADEIQRRVLTEILSKNAHVEYLQQHGLDGSTDAS--T 63
           A  T +  + ++ L+H+E VT NA + QRRVL EIL++NA  EYL++ G+ G    +   
Sbjct: 14  AAATAMCAEHREKLEHIERVTRNAGQEQRRVLEEILAQNAQAEYLRRLGVPGDAPGADEA 73

Query: 64  FKNLVPIVSYEHLKPYIDRIANGDDSPILCAHPITEFLISSGTSGGEQKLFPMFEQELVR 123
           F+ L P+V+YE + P + RIANGD SPIL   P++EFL SSGTSGGE+KL P  E+E+ R
Sbjct: 74  FRRLAPLVTYEDILPDVLRIANGDTSPILSGKPVSEFLTSSGTSGGERKLMPTIEEEMER 133

Query: 124 RLSIRNCMRPKIKELFPSLYNGKEMFFHFARAESVTKGGIVARSASTSLSRSSDFNNMES 183
           R  + + + P +    P L  GK M+ +F ++E  T GG+ AR   TS  RS  F     
Sbjct: 134 RSGLYSLLMPVMSRQVPGLDKGKAMYLYFVKSEWRTPGGLPARPVLTSFYRSRYFLERPH 193

Query: 184 GS---YTSPNEIIRCTDAYQSLYCQLLCGLLQREKVVGIGALFASTLVYVSKFLENHWVL 243
                YTSP+E + C DAYQS+Y QL+CGL+ R  V+ +GA+FAS  +   +FLE HW  
Sbjct: 194 DPYTVYTSPDEAVLCEDAYQSMYAQLICGLVHRADVLRVGAVFASGFLRAIRFLEKHWPS 253

Query: 244 LANDIRTGTVNTKITDQLVRESVMNILK-PDPQLADFIESECSKGTWEGIVTRLWPNTKF 303
           L  DIR G ++  +TD  VR +V  +L+  DP LAD IE+EC++ +W+GI+ R+WP+TK+
Sbjct: 254 LCRDIRAGELDGGVTDPAVRGAVGRVLRGADPALADAIEAECARPSWQGIIRRVWPSTKY 313

Query: 304 INAIVTGSMSQYIPQLNYYSNCLPLISLFYASSECYFGINLDPLSKPEEISYTFIPTMAY 363
           I+ IVTG+M+QYIP L +Y   LPL    YASSECYFG+NL+P+ KP E++YT IPTM Y
Sbjct: 314 IDVIVTGAMAQYIPTLEFYGGGLPLACTMYASSECYFGLNLNPMCKPSEVAYTLIPTMCY 373

Query: 364 FEFLPVDRANANREVGQE-----LVDLVDVKLGQEYEVIVTTYTGLYRYCIGDIVRVTGF 423
           FEFLPV+ + AN     E     LVDLVDVKLG EYE++VTTY+GLYRY +GD++RV GF
Sbjct: 374 FEFLPVN-SGANDVAAPEPDHRGLVDLVDVKLGHEYELVVTTYSGLYRYRVGDVLRVAGF 433

Query: 424 KYKAPCFSFVCRKNVVLSLDFEKTDEAELHNAVKGAGELLNPFGATIADYTSYADN-STF 483
           K  AP F+FV RKNV LS+D +KTDEAELH AV  A + L PFGA++ +YTSYAD  +T 
Sbjct: 434 KNAAPMFAFVRRKNVALSIDSDKTDEAELHAAVTEAVQHLAPFGASLVEYTSYADTATTI 493

Query: 484 PGHYVLYWEIHFKEEISIIPSSIYEDCCLAVEESLNSLYRQARSHQKVINPLEIKMVEAG 543
           PGHYVL+WE+      + +P+S++EDCCLAVEE LNS+YRQ R+  + I PLEI++V  G
Sbjct: 494 PGHYVLFWELRSPAGGTPVPASVFEDCCLAVEEGLNSVYRQCRAADRSIGPLEIRVVADG 553

Query: 544 TFDKLLERATYQGASISQYKTPRCVTSTQIVELLDTKVVASYFSKKYPKW 582
           TFDKL++ A  +GASI+QYK PRCV    +VELLD +V A+YFS K PKW
Sbjct: 554 TFDKLMDYALSRGASINQYKAPRCVRPGPVVELLDGRVQATYFSPKCPKW 602

BLAST of MC02g_new0100 vs. ExPASy Swiss-Prot
Match: O82333 (Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Arabidopsis thaliana OX=3702 GN=GH3.1 PE=2 SV=1)

HSP 1 Score: 605.9 bits (1561), Expect = 4.9e-172
Identity = 301/592 (50.84%), Postives = 408/592 (68.92%), Query Frame = 0

Query: 1   MSNALDTTLDEKDKQVLQHLEDVTSNADEIQRRVLTEILSKNAHVEYLQQHGLDGSTDAS 60
           +S+ L     EKD + L+ +E++T NAD +Q  +L EIL++NA  EYL++  L G+TD  
Sbjct: 7   LSSPLGPPACEKDAKALRFIEEMTRNADTVQENLLAEILARNADTEYLRRFNLCGATDRD 66

Query: 61  TFKNLVPIVSYEHLKPYIDRIANGDDSPILCAHPITEFLISSGTSGGEQKLFPMFEQELV 120
           TFK  +P+++YE L+P I RIA+GD SPIL AHPI+EFL SSGTS GE+KL P  ++EL 
Sbjct: 67  TFKTKIPVITYEDLQPEIQRIADGDRSPILSAHPISEFLTSSGTSAGERKLMPTIKEELD 126

Query: 121 RRLSIRNCMRPKIKELFPSLYNGKEMFFHFARAESVTKGGIVARSASTSLSRSSDFNNM- 180
           RR  + + + P +    P L  GK M+F F ++E+ T GG+ AR   TS  +S  F +  
Sbjct: 127 RRQLLYSLLMPVMNLYVPGLDKGKGMYFLFVKSETKTPGGLPARPVLTSYYKSEHFRSRP 186

Query: 181 --ESGSYTSPNEIIRCTDAYQSLYCQLLCGLLQREKVVGIGALFASTLVYVSKFLENHWV 240
                 YTSPNE I C D++QS+Y Q+LCGLL R  V+ +GA+FAS L+   +FL+ HW 
Sbjct: 187 YDPYNVYTSPNEAILCPDSFQSMYTQMLCGLLDRLSVLRVGAVFASGLLRAIRFLQLHWS 246

Query: 241 LLANDIRTGTVNTKITDQLVRESVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKF 300
             A+DI  G ++++ITD  +R+ +  ILKPDP LA+FI  EC    WE I+TR+WPNTK+
Sbjct: 247 RFAHDIELGCLDSEITDPSIRQCMSGILKPDPVLAEFIRRECKSDNWEKIITRIWPNTKY 306

Query: 301 INAIVTGSMSQYIPQLNYYSNCLPLISLFYASSECYFGINLDPLSKPEEISYTFIPTMAY 360
           ++ IVTG+M+QYIP L YYS  LP+    YASSECYFG+NL+P+SKP E+SYT +P MAY
Sbjct: 307 LDVIVTGAMAQYIPTLEYYSGGLPMACTMYASSECYFGLNLNPMSKPSEVSYTIMPNMAY 366

Query: 361 FEFLPVDRANANREVGQELVDLVDVKLGQEYEVIVTTYTGLYRYCIGDIVRVTGFKYKAP 420
           FEF+P+         G + V+LVDV +G+EYE++VTTY GL RY +GDI+RVTGF   AP
Sbjct: 367 FEFIPLG--------GTKAVELVDVNIGKEYELVVTTYAGLCRYRVGDILRVTGFHNSAP 426

Query: 421 CFSFVCRKNVVLSLDFEKTDEAELHNAVKGAGELLN-PFGATIADYTSYADNSTFPGHYV 480
            F FV RKNV+LS+D +KTDE+EL  AV+ A  +L+   G+ +A+YTSYAD ST PGHYV
Sbjct: 427 QFHFVRRKNVLLSIDSDKTDESELQKAVENASSILHEECGSRVAEYTSYADTSTIPGHYV 486

Query: 481 LYWEIHFKEEISIIPSSIYEDCCLAVEESLNSLYRQARSHQKVINPLEIKMVEAGTFDKL 540
           LYWE+  ++            CCL +EESLNS+YRQ+R     + PLEI++V  GTF++L
Sbjct: 487 LYWELLVRDGARQPSHETLTRCCLGMEESLNSVYRQSRVADNSVGPLEIRVVRNGTFEEL 546

Query: 541 LERATYQGASISQYKTPRCVTSTQIVELLDTKVVASYFSKKYPKWDPTCRHR 589
           ++ A  +GASI+QYK PRCV  T IVELLD++VV+++FS   P W P  R R
Sbjct: 547 MDYAISRGASINQYKVPRCVNFTPIVELLDSRVVSAHFSPSLPHWTPERRRR 590

BLAST of MC02g_new0100 vs. NCBI nr
Match: XP_022140614.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Momordica charantia])

HSP 1 Score: 1045 bits (2703), Expect = 0.0
Identity = 530/594 (89.23%), Postives = 538/594 (90.57%), Query Frame = 0

Query: 7   TTLDEKDKQVLQHLEDVTSNADEIQRRVLTEILSKNAHVEYLQQHGLDGSTDASTFKNLV 66
           + LDEKDKQVLQ+LEDVTSNADEIQRRVL EILS NAHVEYL+QHGLDGSTD STFKNL+
Sbjct: 2   SNLDEKDKQVLQNLEDVTSNADEIQRRVLAEILSTNAHVEYLKQHGLDGSTDVSTFKNLI 61

Query: 67  PIVSYEHLKPYIDRIANGDDSPILCAHPITEFLISSGTSGGEQKLFPMFEQELVRRLSIR 126
           P+VSYEHLKPYIDRI++GDDSPILCAHPIT+F                            
Sbjct: 62  PLVSYEHLKPYIDRISSGDDSPILCAHPITDF---------------------------- 121

Query: 127 NCMRPKIKELFPSLYNGKEMFFHFARAESVTKGGIVARSASTSLSRSSDFNNMESGSYTS 186
                                   ARAESVTKGGIVARSASTSLSRSSDFNNMESGSYTS
Sbjct: 122 ------------------------ARAESVTKGGIVARSASTSLSRSSDFNNMESGSYTS 181

Query: 187 PNEIIRCTDAYQSLYCQLLCGLLQREKVVGIGALFASTLVYVSKFLENHWVLLANDIRTG 246
           PNEIIRCTDAYQSLYCQLLCGLLQREKVVGIGALFASTLVYVSKFLENHWVLLANDIRTG
Sbjct: 182 PNEIIRCTDAYQSLYCQLLCGLLQREKVVGIGALFASTLVYVSKFLENHWVLLANDIRTG 241

Query: 247 TVNTKITDQLVRESVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAIVTGSM 306
           TVNTKITDQLVRESVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAIVTGSM
Sbjct: 242 TVNTKITDQLVRESVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAIVTGSM 301

Query: 307 SQYIPQLNYYSNCLPLISLFYASSECYFGINLDPLSKPEEISYTFIPTMAYFEFLPVDRA 366
           SQYIPQLNYYSNCLPLISLFYASSECYFGINLDPLSKPEEISYTFIPTMAYFEFLPVDRA
Sbjct: 302 SQYIPQLNYYSNCLPLISLFYASSECYFGINLDPLSKPEEISYTFIPTMAYFEFLPVDRA 361

Query: 367 NANREVGQELVDLVDVKLGQEYEVIVTTYTGLYRYCIGDIVRVTGFKYKAPCFSFVCRKN 426
           NANREVGQELVDLVDVKLGQEYEVIVTTYTGLYRYCIGDIVRVTGFKYKAPCFSFVCRKN
Sbjct: 362 NANREVGQELVDLVDVKLGQEYEVIVTTYTGLYRYCIGDIVRVTGFKYKAPCFSFVCRKN 421

Query: 427 VVLSLDFEKTDEAELHNAVKGAGELLNPFGATIADYTSYADNSTFPGHYVLYWEIHFKEE 486
           VVLSLDFEKTDEAELHNAVKGAGELLNPFGATIADYTSYADNSTFPGHYVLYWEIHFKEE
Sbjct: 422 VVLSLDFEKTDEAELHNAVKGAGELLNPFGATIADYTSYADNSTFPGHYVLYWEIHFKEE 481

Query: 487 ISIIPSSIYEDCCLAVEESLNSLYRQARSHQKVINPLEIKMVEAGTFDKLLERATYQGAS 546
           ISIIPSSIYEDCCLAVEESLNSLYRQARSHQKVINPLEIKMVEAGTFDKLLERATYQGAS
Sbjct: 482 ISIIPSSIYEDCCLAVEESLNSLYRQARSHQKVINPLEIKMVEAGTFDKLLERATYQGAS 541

Query: 547 ISQYKTPRCVTSTQIVELLDTKVVASYFSKKYPKWDPTCRHRLFALCQYPYLMR 600
           ISQYKTPRCVTSTQIVELLDTKVVASYFSKKYPKWDPTCRHRLFALCQYPYLMR
Sbjct: 542 ISQYKTPRCVTSTQIVELLDTKVVASYFSKKYPKWDPTCRHRLFALCQYPYLMR 543

BLAST of MC02g_new0100 vs. NCBI nr
Match: XP_022140778.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Momordica charantia])

HSP 1 Score: 950 bits (2455), Expect = 0.0
Identity = 467/582 (80.24%), Postives = 512/582 (87.97%), Query Frame = 0

Query: 7   TTLDEKDKQVLQHLEDVTSNADEIQRRVLTEILSKNAHVEYLQQHGLDGSTDASTFKNLV 66
           + LDEKDKQ LQ LEDVT  ADEIQRRVLTEILS NAHVEYLQQHGL+GSTDASTFKNL+
Sbjct: 2   SNLDEKDKQALQDLEDVTRTADEIQRRVLTEILSTNAHVEYLQQHGLNGSTDASTFKNLI 61

Query: 67  PIVSYEHLKPYIDRIANGDDSPILCAHPITEFLISSGTSGGEQKLFPMFEQELVRRLSIR 126
           PIVSYEHLKPYIDRI NGDDSPILCAHPITEF  S+GTSGGEQKL P  EQE  R+LSI 
Sbjct: 62  PIVSYEHLKPYIDRIVNGDDSPILCAHPITEFYTSTGTSGGEQKLIPTIEQEFARKLSIS 121

Query: 127 NCMRPKIKELFPSLYNGKEMFFHFARAESVTKGGIVARSASTSLSRSSDFNNME-SGSYT 186
           N MRP++K+LFP L+NGKEMFF+FA+ ES+TK GIVARS STS  +S   NN++   SYT
Sbjct: 122 NYMRPRMKQLFPGLHNGKEMFFNFAKVESITKAGIVARSVSTSFLKSPHLNNVKVESSYT 181

Query: 187 SPNEIIRCTDAYQSLYCQLLCGLLQREKVVGIGALFASTLVYVSKFLENHWVLLANDIRT 246
           SP +I+ CTD YQSLYCQLLCGL Q +KVV +G+LFASTL+Y+ KFLENHWVLLANDIRT
Sbjct: 182 SPTDILCCTDVYQSLYCQLLCGLCQNQKVVRVGSLFASTLIYIFKFLENHWVLLANDIRT 241

Query: 247 GTVNTKITDQLVRESVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAIVTGS 306
           GT+N KI D  VR+SVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINA+VTGS
Sbjct: 242 GTINAKIMDSYVRKSVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAVVTGS 301

Query: 307 MSQYIPQLNYYSNCLPLISLFYASSECYFGINLDPLSKPEEISYTFIPTMAYFEFLPVDR 366
           MS YIPQLNYY   LPLI++FYASSEC+FGINLDPL KPEEISYTFIPT+AYFEFLPVDR
Sbjct: 302 MSHYIPQLNYYCKSLPLITVFYASSECFFGINLDPLCKPEEISYTFIPTVAYFEFLPVDR 361

Query: 367 ANANREVGQELVDLVDVKLGQEYEVIVTTYTGLYRYCIGDIVRVTGFKYKAPCFSFVCRK 426
            N + EVGQE VDLVDVKLGQEYE++VTTY GLYRYCIGDIVRVTGFK KAP F FV RK
Sbjct: 362 TNDSGEVGQEFVDLVDVKLGQEYEIVVTTYAGLYRYCIGDIVRVTGFKNKAPNFRFVGRK 421

Query: 427 NVVLSLDFEKTDEAELHNAVKGAGELLNPFGATIADYTSYADNSTFPGHYVLYWEIHFKE 486
           NV+LSLD EKTDEAELHNAV   G LL+ FGA + DYTSYAD+STFPGHYVLYWEI FKE
Sbjct: 422 NVILSLDVEKTDEAELHNAVNKVGSLLSSFGAILVDYTSYADSSTFPGHYVLYWEIQFKE 481

Query: 487 EISIIPSSIYEDCCLAVEESLNSLYRQARSHQKVINPLEIKMVEAGTFDKLLERATYQGA 546
           EISIIPSSIYEDCCLA+EESL+S+YR+ARSHQKVINPLEIK+VEA TFDKL+ER   QGA
Sbjct: 482 EISIIPSSIYEDCCLAIEESLSSVYRRARSHQKVINPLEIKIVEARTFDKLMERMVDQGA 541

Query: 547 SISQYKTPRCVTSTQIVELLDTKVVASYFSKKYPKWDPTCRH 587
           SISQYKTPRCV STQI+E LD+KVVA+YFSKKYPKWDPT  H
Sbjct: 542 SISQYKTPRCVKSTQIIEFLDSKVVANYFSKKYPKWDPTRPH 583

BLAST of MC02g_new0100 vs. NCBI nr
Match: XP_022140368.1 (indole-3-acetic acid-amido synthetase GH3.5-like [Momordica charantia])

HSP 1 Score: 871 bits (2250), Expect = 8.50e-314
Identity = 429/577 (74.35%), Postives = 501/577 (86.83%), Query Frame = 0

Query: 6   DTTLDEKDKQVLQHLEDVTSNADEIQRRVLTEILSKNAHVEYLQQHGLDGSTDASTFKNL 65
           D  LD KDKQ+L H+ED TSNADEIQRRV+TEILSKNAHVEYL+Q GLDGSTD STFK L
Sbjct: 3   DDILDAKDKQILYHIEDFTSNADEIQRRVITEILSKNAHVEYLKQLGLDGSTDISTFKKL 62

Query: 66  VPIVSYEHLKPYIDRIANGDDSPILCAHPITEFLISSGTSGGEQKLFPMFEQELVRRLSI 125
           +P+VSYE LKPYIDRIA+GDDSPILCA  IT+FLIS+GTSGG QKL PMF+ EL+RRL+ 
Sbjct: 63  IPVVSYEQLKPYIDRIADGDDSPILCADTITDFLISTGTSGGAQKLIPMFQSELLRRLAT 122

Query: 126 RNCMRPKIKELFPSLYNGKEMFFHFARAESVTKGGIVARSASTSLSRSSDFNNMES-GSY 185
                P +K+LFP L NGKEMFF+F RAE+ TKGG+VA+  S+ LS+ S F+++E   +Y
Sbjct: 123 NYYAIPMMKQLFPGLLNGKEMFFNFTRAETKTKGGMVAQLLSSCLSKCSLFSSVEVLRAY 182

Query: 186 TSPNEIIRCTDAYQSLYCQLLCGLLQREKVVGIGALFASTLVYVSKFLENHWVLLANDIR 245
           TSP +IIRCTD YQSLYCQLLCGL Q + V+ +GALFASTL+Y+ KFLENHW LL NDIR
Sbjct: 183 TSPIDIIRCTDVYQSLYCQLLCGLCQNQIVLRVGALFASTLIYIFKFLENHWALLVNDIR 242

Query: 246 TGTVNTKITDQLVRESVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAIVTG 305
           TGTV+ KI D LVRESVM++LKP+P+LADFIESECSKG WEGIVTRLWPNTK+I+ +VTG
Sbjct: 243 TGTVDAKIEDPLVRESVMSVLKPNPELADFIESECSKGAWEGIVTRLWPNTKYISTVVTG 302

Query: 306 SMSQYIPQLNYYSNCLPLISLFYASSECYFGINLDPLSKPEEISYTFIPTMAYFEFLPVD 365
           SMSQYIPQLN+Y  C P++S+FYASSEC+FGINLDPL KPEEISYTF+PTMAYFEFLPVD
Sbjct: 303 SMSQYIPQLNFYCKC-PIVSVFYASSECFFGINLDPLCKPEEISYTFMPTMAYFEFLPVD 362

Query: 366 RANANR-EVGQELVDLVDVKLGQEYEVIVTTYTGLYRYCIGDIVRVTGFKYKAPCFSFVC 425
           RAN+N  EVGQELVDLVDVKLGQEYEV+VTTY GLYRYC+GDI+RVTGFK K P FSF C
Sbjct: 363 RANSNNGEVGQELVDLVDVKLGQEYEVVVTTYAGLYRYCMGDILRVTGFKNKTPNFSFAC 422

Query: 426 RKNVVLSLDFEKTDEAELHNAVKGAGELLNPFGATIADYTSYADNSTFPGHYVLYWEIHF 485
           RKNV+LSLD EKTDE +LH+AV  A  LL+P GA I DYTSYAD+STFPGHYVLYWEI  
Sbjct: 423 RKNVILSLDIEKTDEVDLHHAVTEASGLLSPLGAIIVDYTSYADSSTFPGHYVLYWEIQC 482

Query: 486 KEEISIIPSSIYEDCCLAVEESLNSLYRQARSHQKVINPLEIKMVEAGTFDKLLERATYQ 545
           +EE S IPS++YEDCCLAVEESL+S+YRQARS+ KVINPLEIK+VE GTF+KL+E+A  +
Sbjct: 483 REETSTIPSTVYEDCCLAVEESLSSVYRQARSNNKVINPLEIKIVETGTFEKLMEQAVDR 542

Query: 546 GASISQYKTPRCVTSTQIVELLDTKVVASYFSKKYPK 580
           GASISQYKTPRC+ STQ++ELLD+KVV++YFS++YPK
Sbjct: 543 GASISQYKTPRCLKSTQMIELLDSKVVSNYFSQEYPK 578

BLAST of MC02g_new0100 vs. NCBI nr
Match: KAG6570863.1 (Indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 811 bits (2096), Expect = 3.12e-290
Identity = 399/593 (67.28%), Postives = 483/593 (81.45%), Query Frame = 0

Query: 1   MSNALDTTLDEKDKQVLQHLEDVTSNADEIQRRVLTEILSKNAHVEYLQQHGLDGSTDAS 60
           M++  + + DE+DKQ LQH+EDVT +A +IQR++LT+ILS NA VEYLQQHGL GST+ S
Sbjct: 1   MAHVDEISSDERDKQALQHIEDVTIHASQIQRQILTQILSTNADVEYLQQHGLHGSTELS 60

Query: 61  TFKNLVPIVSYEHLKPYIDRIANGDDSPILCAHPITEFLISSGTSGGEQKLFPMFEQELV 120
           TFKNL+P++SYE L+PYIDRIANGDDSPILC +PITEF ISSGTSGGE KL PM+E+E +
Sbjct: 61  TFKNLIPLISYEQLRPYIDRIANGDDSPILCTNPITEFFISSGTSGGEPKLIPMYEEEFM 120

Query: 121 RRLSIRNCMRPKIKELFPS--LYNGKEMFFHFARAESVTKGGIVARSASTSLSRSSDFNN 180
           RRLS  + + P++K+LFP+  +  GK M F FA+ E  T+ GIVA +A+TSL + ++ NN
Sbjct: 121 RRLSFFDYVMPRMKQLFPNSLVGKGKGMHFQFAKVELKTRSGIVAHTATTSLCKRANLNN 180

Query: 181 MESGSYTSPNEIIRCTDAYQSLYCQLLCGLLQREKVVGIGALFASTLVYVSKFLENHWVL 240
                 TSP +II CTD  QSLYCQLLCGL Q + V  +GALFASTL++V KFLE+HWV+
Sbjct: 181 ------TSPKDIILCTDTNQSLYCQLLCGLCQNDIVFRVGALFASTLIHVLKFLEDHWVV 240

Query: 241 LANDIRTGTVNTKITDQLVRESVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFI 300
           LA+DIR GT+NTKITD  VRE+VMNIL P+ QLADFIE+ECSKGTWEGI+TRLWPN K+I
Sbjct: 241 LASDIRAGTLNTKITDPSVREAVMNILNPNAQLADFIEAECSKGTWEGIITRLWPNAKYI 300

Query: 301 NAIVTGSMSQYIPQLNYYSNCLPLISLFYASSECYFGINLDPLSKPEEISYTFIPTMAYF 360
           NAIVTGSM+QY+P+LNYYSN LP++S  Y  SEC+ G+NLDPL  P+  SYT IPTMAYF
Sbjct: 301 NAIVTGSMAQYLPKLNYYSNHLPIVSDHYGGSECFLGLNLDPLCSPDRTSYTLIPTMAYF 360

Query: 361 EFLPVDRANANREVGQELVDLVDVKLGQEYEVIVTTYTGLYRYCIGDIVRVTGFKYKAPC 420
           EFLP+DR  ++RE+ +ELVDLVDVKLG EYE+++TTY GLYRYC+GDI+RVTGFK KAP 
Sbjct: 361 EFLPIDRTKSSRELSRELVDLVDVKLGHEYELVITTYAGLYRYCMGDILRVTGFKNKAPN 420

Query: 421 FSFVCRKNVVLSLDFEKTDEAELHNAVKGAGELLNPFGATIADYTSYADNSTFPGHYVLY 480
           FSFVCRKNVVLSLD EKTDEAELH+AV+ A  LL+PFGA IADYTS+AD+S  PGHYVLY
Sbjct: 421 FSFVCRKNVVLSLDIEKTDEAELHDAVQEAARLLSPFGAAIADYTSHADSSKIPGHYVLY 480

Query: 481 WEIHFKEEISIIPSSIYEDCCLAVEESLNSLYRQARSHQKVINPLEIKMVEAGTFDKLLE 540
           WEIH K  ISI PSSIYEDCC A+EE LN  YR  RSH+K INPLEIK+V+ GTF+KL+ 
Sbjct: 481 WEIHVKGGISI-PSSIYEDCCFAIEELLNGPYRYLRSHEKCINPLEIKIVKVGTFEKLMA 540

Query: 541 RATYQGASISQYKTPRCVTSTQIVELLDTKVVASYFSKKYPKWDPTCRHRLFA 591
           +A  +GAS+SQYKTPRCV ST I+ELLD+ VV  YFSK+ PK D    HRL +
Sbjct: 541 QALNRGASMSQYKTPRCVNSTHIIELLDSNVVTKYFSKECPKVDTNALHRLLS 586

BLAST of MC02g_new0100 vs. NCBI nr
Match: XP_022944596.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita moschata])

HSP 1 Score: 805 bits (2080), Expect = 8.14e-288
Identity = 398/593 (67.12%), Postives = 482/593 (81.28%), Query Frame = 0

Query: 1   MSNALDTTLDEKDKQVLQHLEDVTSNADEIQRRVLTEILSKNAHVEYLQQHGLDGSTDAS 60
           M++  + + DE+DKQ LQH+EDVT +A +IQR++LT+ILS NA VEYLQQHGL GST+ S
Sbjct: 1   MAHVDEISSDERDKQALQHIEDVTIHASQIQRQILTQILSTNADVEYLQQHGLHGSTELS 60

Query: 61  TFKNLVPIVSYEHLKPYIDRIANGDDSPILCAHPITEFLISSGTSGGEQKLFPMFEQELV 120
           TFKNL+P++SYE L+PYIDRIANGDDSPILC +PITEF ISSGTSGGE KL PM+E+E +
Sbjct: 61  TFKNLIPLISYEQLRPYIDRIANGDDSPILCTNPITEFFISSGTSGGEPKLIPMYEEEFM 120

Query: 121 RRLSIRNCMRPKIKELFPS--LYNGKEMFFHFARAESVTKGGIVARSASTSLSRSSDFNN 180
           RRLS  + + P++K+LFP+  +  GK M F FA+ E  T+ GIVA +A+TSL + ++ NN
Sbjct: 121 RRLSFFDYVMPRMKQLFPNSLVGKGKGMHFQFAKVELKTRSGIVAHTATTSLCKRANLNN 180

Query: 181 MESGSYTSPNEIIRCTDAYQSLYCQLLCGLLQREKVVGIGALFASTLVYVSKFLENHWVL 240
                 TSP +II CTD  QSLYCQLLCGL Q + V  +GALFASTL++V KFLE+HWV+
Sbjct: 181 ------TSPKDIILCTDTNQSLYCQLLCGLCQNDIVFRVGALFASTLIHVLKFLEDHWVV 240

Query: 241 LANDIRTGTVNTKITDQLVRESVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFI 300
           LA+DIR GT+NTKITD  VRE+VMNIL P+ QLADFIE+ECSKGTWEGI+TRLWPN K+I
Sbjct: 241 LASDIRAGTLNTKITDPSVREAVMNILNPNAQLADFIEAECSKGTWEGIITRLWPNAKYI 300

Query: 301 NAIVTGSMSQYIPQLNYYSNCLPLISLFYASSECYFGINLDPLSKPEEISYTFIPTMAYF 360
           NAIVTGSM+QY+P+LNYYSN LP++S  Y +SEC+ G+NLDPL  P+  SYT IPTMAYF
Sbjct: 301 NAIVTGSMAQYLPKLNYYSNHLPIVSDHYGASECFLGLNLDPLCSPDRTSYTLIPTMAYF 360

Query: 361 EFLPVDRANANREVGQELVDLVDVKLGQEYEVIVTTYTGLYRYCIGDIVRVTGFKYKAPC 420
           EFLP+DR  ++RE+ +ELVDLVDVKLG EYE+++TTY GLYRY +GDI+ VTGFK KAP 
Sbjct: 361 EFLPIDRTKSSRELSRELVDLVDVKLGHEYELVITTYAGLYRYRMGDILGVTGFKNKAPN 420

Query: 421 FSFVCRKNVVLSLDFEKTDEAELHNAVKGAGELLNPFGATIADYTSYADNSTFPGHYVLY 480
           FSFVCRKNVVLSLD EKTDEAELH+AV+ A  LL+PFGA IADYTS+AD+S  PGHYVLY
Sbjct: 421 FSFVCRKNVVLSLDIEKTDEAELHDAVQEAARLLSPFGAAIADYTSHADSSKIPGHYVLY 480

Query: 481 WEIHFKEEISIIPSSIYEDCCLAVEESLNSLYRQARSHQKVINPLEIKMVEAGTFDKLLE 540
           WEIH K  ISI PSSIYEDCC A+EE LN  YR  RSH+K INPLEIK+V+ GTF+KL+ 
Sbjct: 481 WEIHVKGGISI-PSSIYEDCCFAIEELLNGPYRYLRSHEKCINPLEIKIVKVGTFEKLMA 540

Query: 541 RATYQGASISQYKTPRCVTSTQIVELLDTKVVASYFSKKYPKWDPTCRHRLFA 591
           +A  +GAS+SQYKTPRCV ST I+ELLD+ VV  YFSKK PK D    HRL +
Sbjct: 541 QALNRGASMSQYKTPRCVNSTHIIELLDSNVVIKYFSKKCPKVDTKALHRLLS 586

BLAST of MC02g_new0100 vs. ExPASy TrEMBL
Match: A0A6J1CG61 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Momordica charantia OX=3673 GN=LOC111011222 PE=3 SV=1)

HSP 1 Score: 1045 bits (2703), Expect = 0.0
Identity = 530/594 (89.23%), Postives = 538/594 (90.57%), Query Frame = 0

Query: 7   TTLDEKDKQVLQHLEDVTSNADEIQRRVLTEILSKNAHVEYLQQHGLDGSTDASTFKNLV 66
           + LDEKDKQVLQ+LEDVTSNADEIQRRVL EILS NAHVEYL+QHGLDGSTD STFKNL+
Sbjct: 2   SNLDEKDKQVLQNLEDVTSNADEIQRRVLAEILSTNAHVEYLKQHGLDGSTDVSTFKNLI 61

Query: 67  PIVSYEHLKPYIDRIANGDDSPILCAHPITEFLISSGTSGGEQKLFPMFEQELVRRLSIR 126
           P+VSYEHLKPYIDRI++GDDSPILCAHPIT+F                            
Sbjct: 62  PLVSYEHLKPYIDRISSGDDSPILCAHPITDF---------------------------- 121

Query: 127 NCMRPKIKELFPSLYNGKEMFFHFARAESVTKGGIVARSASTSLSRSSDFNNMESGSYTS 186
                                   ARAESVTKGGIVARSASTSLSRSSDFNNMESGSYTS
Sbjct: 122 ------------------------ARAESVTKGGIVARSASTSLSRSSDFNNMESGSYTS 181

Query: 187 PNEIIRCTDAYQSLYCQLLCGLLQREKVVGIGALFASTLVYVSKFLENHWVLLANDIRTG 246
           PNEIIRCTDAYQSLYCQLLCGLLQREKVVGIGALFASTLVYVSKFLENHWVLLANDIRTG
Sbjct: 182 PNEIIRCTDAYQSLYCQLLCGLLQREKVVGIGALFASTLVYVSKFLENHWVLLANDIRTG 241

Query: 247 TVNTKITDQLVRESVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAIVTGSM 306
           TVNTKITDQLVRESVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAIVTGSM
Sbjct: 242 TVNTKITDQLVRESVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAIVTGSM 301

Query: 307 SQYIPQLNYYSNCLPLISLFYASSECYFGINLDPLSKPEEISYTFIPTMAYFEFLPVDRA 366
           SQYIPQLNYYSNCLPLISLFYASSECYFGINLDPLSKPEEISYTFIPTMAYFEFLPVDRA
Sbjct: 302 SQYIPQLNYYSNCLPLISLFYASSECYFGINLDPLSKPEEISYTFIPTMAYFEFLPVDRA 361

Query: 367 NANREVGQELVDLVDVKLGQEYEVIVTTYTGLYRYCIGDIVRVTGFKYKAPCFSFVCRKN 426
           NANREVGQELVDLVDVKLGQEYEVIVTTYTGLYRYCIGDIVRVTGFKYKAPCFSFVCRKN
Sbjct: 362 NANREVGQELVDLVDVKLGQEYEVIVTTYTGLYRYCIGDIVRVTGFKYKAPCFSFVCRKN 421

Query: 427 VVLSLDFEKTDEAELHNAVKGAGELLNPFGATIADYTSYADNSTFPGHYVLYWEIHFKEE 486
           VVLSLDFEKTDEAELHNAVKGAGELLNPFGATIADYTSYADNSTFPGHYVLYWEIHFKEE
Sbjct: 422 VVLSLDFEKTDEAELHNAVKGAGELLNPFGATIADYTSYADNSTFPGHYVLYWEIHFKEE 481

Query: 487 ISIIPSSIYEDCCLAVEESLNSLYRQARSHQKVINPLEIKMVEAGTFDKLLERATYQGAS 546
           ISIIPSSIYEDCCLAVEESLNSLYRQARSHQKVINPLEIKMVEAGTFDKLLERATYQGAS
Sbjct: 482 ISIIPSSIYEDCCLAVEESLNSLYRQARSHQKVINPLEIKMVEAGTFDKLLERATYQGAS 541

Query: 547 ISQYKTPRCVTSTQIVELLDTKVVASYFSKKYPKWDPTCRHRLFALCQYPYLMR 600
           ISQYKTPRCVTSTQIVELLDTKVVASYFSKKYPKWDPTCRHRLFALCQYPYLMR
Sbjct: 542 ISQYKTPRCVTSTQIVELLDTKVVASYFSKKYPKWDPTCRHRLFALCQYPYLMR 543

BLAST of MC02g_new0100 vs. ExPASy TrEMBL
Match: A0A6J1CIS9 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Momordica charantia OX=3673 GN=LOC111011358 PE=3 SV=1)

HSP 1 Score: 950 bits (2455), Expect = 0.0
Identity = 467/582 (80.24%), Postives = 512/582 (87.97%), Query Frame = 0

Query: 7   TTLDEKDKQVLQHLEDVTSNADEIQRRVLTEILSKNAHVEYLQQHGLDGSTDASTFKNLV 66
           + LDEKDKQ LQ LEDVT  ADEIQRRVLTEILS NAHVEYLQQHGL+GSTDASTFKNL+
Sbjct: 2   SNLDEKDKQALQDLEDVTRTADEIQRRVLTEILSTNAHVEYLQQHGLNGSTDASTFKNLI 61

Query: 67  PIVSYEHLKPYIDRIANGDDSPILCAHPITEFLISSGTSGGEQKLFPMFEQELVRRLSIR 126
           PIVSYEHLKPYIDRI NGDDSPILCAHPITEF  S+GTSGGEQKL P  EQE  R+LSI 
Sbjct: 62  PIVSYEHLKPYIDRIVNGDDSPILCAHPITEFYTSTGTSGGEQKLIPTIEQEFARKLSIS 121

Query: 127 NCMRPKIKELFPSLYNGKEMFFHFARAESVTKGGIVARSASTSLSRSSDFNNME-SGSYT 186
           N MRP++K+LFP L+NGKEMFF+FA+ ES+TK GIVARS STS  +S   NN++   SYT
Sbjct: 122 NYMRPRMKQLFPGLHNGKEMFFNFAKVESITKAGIVARSVSTSFLKSPHLNNVKVESSYT 181

Query: 187 SPNEIIRCTDAYQSLYCQLLCGLLQREKVVGIGALFASTLVYVSKFLENHWVLLANDIRT 246
           SP +I+ CTD YQSLYCQLLCGL Q +KVV +G+LFASTL+Y+ KFLENHWVLLANDIRT
Sbjct: 182 SPTDILCCTDVYQSLYCQLLCGLCQNQKVVRVGSLFASTLIYIFKFLENHWVLLANDIRT 241

Query: 247 GTVNTKITDQLVRESVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAIVTGS 306
           GT+N KI D  VR+SVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINA+VTGS
Sbjct: 242 GTINAKIMDSYVRKSVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAVVTGS 301

Query: 307 MSQYIPQLNYYSNCLPLISLFYASSECYFGINLDPLSKPEEISYTFIPTMAYFEFLPVDR 366
           MS YIPQLNYY   LPLI++FYASSEC+FGINLDPL KPEEISYTFIPT+AYFEFLPVDR
Sbjct: 302 MSHYIPQLNYYCKSLPLITVFYASSECFFGINLDPLCKPEEISYTFIPTVAYFEFLPVDR 361

Query: 367 ANANREVGQELVDLVDVKLGQEYEVIVTTYTGLYRYCIGDIVRVTGFKYKAPCFSFVCRK 426
            N + EVGQE VDLVDVKLGQEYE++VTTY GLYRYCIGDIVRVTGFK KAP F FV RK
Sbjct: 362 TNDSGEVGQEFVDLVDVKLGQEYEIVVTTYAGLYRYCIGDIVRVTGFKNKAPNFRFVGRK 421

Query: 427 NVVLSLDFEKTDEAELHNAVKGAGELLNPFGATIADYTSYADNSTFPGHYVLYWEIHFKE 486
           NV+LSLD EKTDEAELHNAV   G LL+ FGA + DYTSYAD+STFPGHYVLYWEI FKE
Sbjct: 422 NVILSLDVEKTDEAELHNAVNKVGSLLSSFGAILVDYTSYADSSTFPGHYVLYWEIQFKE 481

Query: 487 EISIIPSSIYEDCCLAVEESLNSLYRQARSHQKVINPLEIKMVEAGTFDKLLERATYQGA 546
           EISIIPSSIYEDCCLA+EESL+S+YR+ARSHQKVINPLEIK+VEA TFDKL+ER   QGA
Sbjct: 482 EISIIPSSIYEDCCLAIEESLSSVYRRARSHQKVINPLEIKIVEARTFDKLMERMVDQGA 541

Query: 547 SISQYKTPRCVTSTQIVELLDTKVVASYFSKKYPKWDPTCRH 587
           SISQYKTPRCV STQI+E LD+KVVA+YFSKKYPKWDPT  H
Sbjct: 542 SISQYKTPRCVKSTQIIEFLDSKVVANYFSKKYPKWDPTRPH 583

BLAST of MC02g_new0100 vs. ExPASy TrEMBL
Match: A0A6J1CHU9 (indole-3-acetic acid-amido synthetase GH3.5-like OS=Momordica charantia OX=3673 GN=LOC111011061 PE=3 SV=1)

HSP 1 Score: 871 bits (2250), Expect = 4.12e-314
Identity = 429/577 (74.35%), Postives = 501/577 (86.83%), Query Frame = 0

Query: 6   DTTLDEKDKQVLQHLEDVTSNADEIQRRVLTEILSKNAHVEYLQQHGLDGSTDASTFKNL 65
           D  LD KDKQ+L H+ED TSNADEIQRRV+TEILSKNAHVEYL+Q GLDGSTD STFK L
Sbjct: 3   DDILDAKDKQILYHIEDFTSNADEIQRRVITEILSKNAHVEYLKQLGLDGSTDISTFKKL 62

Query: 66  VPIVSYEHLKPYIDRIANGDDSPILCAHPITEFLISSGTSGGEQKLFPMFEQELVRRLSI 125
           +P+VSYE LKPYIDRIA+GDDSPILCA  IT+FLIS+GTSGG QKL PMF+ EL+RRL+ 
Sbjct: 63  IPVVSYEQLKPYIDRIADGDDSPILCADTITDFLISTGTSGGAQKLIPMFQSELLRRLAT 122

Query: 126 RNCMRPKIKELFPSLYNGKEMFFHFARAESVTKGGIVARSASTSLSRSSDFNNMES-GSY 185
                P +K+LFP L NGKEMFF+F RAE+ TKGG+VA+  S+ LS+ S F+++E   +Y
Sbjct: 123 NYYAIPMMKQLFPGLLNGKEMFFNFTRAETKTKGGMVAQLLSSCLSKCSLFSSVEVLRAY 182

Query: 186 TSPNEIIRCTDAYQSLYCQLLCGLLQREKVVGIGALFASTLVYVSKFLENHWVLLANDIR 245
           TSP +IIRCTD YQSLYCQLLCGL Q + V+ +GALFASTL+Y+ KFLENHW LL NDIR
Sbjct: 183 TSPIDIIRCTDVYQSLYCQLLCGLCQNQIVLRVGALFASTLIYIFKFLENHWALLVNDIR 242

Query: 246 TGTVNTKITDQLVRESVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAIVTG 305
           TGTV+ KI D LVRESVM++LKP+P+LADFIESECSKG WEGIVTRLWPNTK+I+ +VTG
Sbjct: 243 TGTVDAKIEDPLVRESVMSVLKPNPELADFIESECSKGAWEGIVTRLWPNTKYISTVVTG 302

Query: 306 SMSQYIPQLNYYSNCLPLISLFYASSECYFGINLDPLSKPEEISYTFIPTMAYFEFLPVD 365
           SMSQYIPQLN+Y  C P++S+FYASSEC+FGINLDPL KPEEISYTF+PTMAYFEFLPVD
Sbjct: 303 SMSQYIPQLNFYCKC-PIVSVFYASSECFFGINLDPLCKPEEISYTFMPTMAYFEFLPVD 362

Query: 366 RANANR-EVGQELVDLVDVKLGQEYEVIVTTYTGLYRYCIGDIVRVTGFKYKAPCFSFVC 425
           RAN+N  EVGQELVDLVDVKLGQEYEV+VTTY GLYRYC+GDI+RVTGFK K P FSF C
Sbjct: 363 RANSNNGEVGQELVDLVDVKLGQEYEVVVTTYAGLYRYCMGDILRVTGFKNKTPNFSFAC 422

Query: 426 RKNVVLSLDFEKTDEAELHNAVKGAGELLNPFGATIADYTSYADNSTFPGHYVLYWEIHF 485
           RKNV+LSLD EKTDE +LH+AV  A  LL+P GA I DYTSYAD+STFPGHYVLYWEI  
Sbjct: 423 RKNVILSLDIEKTDEVDLHHAVTEASGLLSPLGAIIVDYTSYADSSTFPGHYVLYWEIQC 482

Query: 486 KEEISIIPSSIYEDCCLAVEESLNSLYRQARSHQKVINPLEIKMVEAGTFDKLLERATYQ 545
           +EE S IPS++YEDCCLAVEESL+S+YRQARS+ KVINPLEIK+VE GTF+KL+E+A  +
Sbjct: 483 REETSTIPSTVYEDCCLAVEESLSSVYRQARSNNKVINPLEIKIVETGTFEKLMEQAVDR 542

Query: 546 GASISQYKTPRCVTSTQIVELLDTKVVASYFSKKYPK 580
           GASISQYKTPRC+ STQ++ELLD+KVV++YFS++YPK
Sbjct: 543 GASISQYKTPRCLKSTQMIELLDSKVVSNYFSQEYPK 578

BLAST of MC02g_new0100 vs. ExPASy TrEMBL
Match: A0A6J1FUU5 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita moschata OX=3662 GN=LOC111449009 PE=3 SV=1)

HSP 1 Score: 805 bits (2080), Expect = 3.94e-288
Identity = 398/593 (67.12%), Postives = 482/593 (81.28%), Query Frame = 0

Query: 1   MSNALDTTLDEKDKQVLQHLEDVTSNADEIQRRVLTEILSKNAHVEYLQQHGLDGSTDAS 60
           M++  + + DE+DKQ LQH+EDVT +A +IQR++LT+ILS NA VEYLQQHGL GST+ S
Sbjct: 1   MAHVDEISSDERDKQALQHIEDVTIHASQIQRQILTQILSTNADVEYLQQHGLHGSTELS 60

Query: 61  TFKNLVPIVSYEHLKPYIDRIANGDDSPILCAHPITEFLISSGTSGGEQKLFPMFEQELV 120
           TFKNL+P++SYE L+PYIDRIANGDDSPILC +PITEF ISSGTSGGE KL PM+E+E +
Sbjct: 61  TFKNLIPLISYEQLRPYIDRIANGDDSPILCTNPITEFFISSGTSGGEPKLIPMYEEEFM 120

Query: 121 RRLSIRNCMRPKIKELFPS--LYNGKEMFFHFARAESVTKGGIVARSASTSLSRSSDFNN 180
           RRLS  + + P++K+LFP+  +  GK M F FA+ E  T+ GIVA +A+TSL + ++ NN
Sbjct: 121 RRLSFFDYVMPRMKQLFPNSLVGKGKGMHFQFAKVELKTRSGIVAHTATTSLCKRANLNN 180

Query: 181 MESGSYTSPNEIIRCTDAYQSLYCQLLCGLLQREKVVGIGALFASTLVYVSKFLENHWVL 240
                 TSP +II CTD  QSLYCQLLCGL Q + V  +GALFASTL++V KFLE+HWV+
Sbjct: 181 ------TSPKDIILCTDTNQSLYCQLLCGLCQNDIVFRVGALFASTLIHVLKFLEDHWVV 240

Query: 241 LANDIRTGTVNTKITDQLVRESVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFI 300
           LA+DIR GT+NTKITD  VRE+VMNIL P+ QLADFIE+ECSKGTWEGI+TRLWPN K+I
Sbjct: 241 LASDIRAGTLNTKITDPSVREAVMNILNPNAQLADFIEAECSKGTWEGIITRLWPNAKYI 300

Query: 301 NAIVTGSMSQYIPQLNYYSNCLPLISLFYASSECYFGINLDPLSKPEEISYTFIPTMAYF 360
           NAIVTGSM+QY+P+LNYYSN LP++S  Y +SEC+ G+NLDPL  P+  SYT IPTMAYF
Sbjct: 301 NAIVTGSMAQYLPKLNYYSNHLPIVSDHYGASECFLGLNLDPLCSPDRTSYTLIPTMAYF 360

Query: 361 EFLPVDRANANREVGQELVDLVDVKLGQEYEVIVTTYTGLYRYCIGDIVRVTGFKYKAPC 420
           EFLP+DR  ++RE+ +ELVDLVDVKLG EYE+++TTY GLYRY +GDI+ VTGFK KAP 
Sbjct: 361 EFLPIDRTKSSRELSRELVDLVDVKLGHEYELVITTYAGLYRYRMGDILGVTGFKNKAPN 420

Query: 421 FSFVCRKNVVLSLDFEKTDEAELHNAVKGAGELLNPFGATIADYTSYADNSTFPGHYVLY 480
           FSFVCRKNVVLSLD EKTDEAELH+AV+ A  LL+PFGA IADYTS+AD+S  PGHYVLY
Sbjct: 421 FSFVCRKNVVLSLDIEKTDEAELHDAVQEAARLLSPFGAAIADYTSHADSSKIPGHYVLY 480

Query: 481 WEIHFKEEISIIPSSIYEDCCLAVEESLNSLYRQARSHQKVINPLEIKMVEAGTFDKLLE 540
           WEIH K  ISI PSSIYEDCC A+EE LN  YR  RSH+K INPLEIK+V+ GTF+KL+ 
Sbjct: 481 WEIHVKGGISI-PSSIYEDCCFAIEELLNGPYRYLRSHEKCINPLEIKIVKVGTFEKLMA 540

Query: 541 RATYQGASISQYKTPRCVTSTQIVELLDTKVVASYFSKKYPKWDPTCRHRLFA 591
           +A  +GAS+SQYKTPRCV ST I+ELLD+ VV  YFSKK PK D    HRL +
Sbjct: 541 QALNRGASMSQYKTPRCVNSTHIIELLDSNVVIKYFSKKCPKVDTKALHRLLS 586

BLAST of MC02g_new0100 vs. ExPASy TrEMBL
Match: A0A5A7V3L5 (Indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold143G00190 PE=3 SV=1)

HSP 1 Score: 764 bits (1974), Expect = 6.31e-272
Identity = 378/592 (63.85%), Postives = 474/592 (80.07%), Query Frame = 0

Query: 1   MSNALDTTLDEKDKQVLQHLEDVTSNADEIQRRVLTEILSKNAHVEYLQQHGLDGSTDAS 60
           MSN  +  LDEKDK+ LQ++ED+T+ ADEIQR++L EILSKNA+VEYLQ+HGLD STD+S
Sbjct: 1   MSNFEEKWLDEKDKEALQYIEDITTKADEIQRQILNEILSKNANVEYLQKHGLDASTDSS 60

Query: 61  TFKNLVPIVSYEHLKPYIDRIANGDDSPILCAHPITEFLISSGTSGGEQKLFPMFEQELV 120
           TFK L+P+V Y+ L+PYI RIA+GDDS ILC++PITEF  SSGTSG E KL PM+EQE V
Sbjct: 61  TFKKLIPLVYYQQLRPYIARIADGDDSSILCSNPITEFFKSSGTSGEEHKLIPMYEQEFV 120

Query: 121 RRLSIRNCMRPKIKELFPSL--YNGKEMFFHFARAESVTKGGIVARSASTSL-SRSSDFN 180
           RRLS  + + P++K+LFP++  +  K + FHFA+ E  TKGGIV RS  T+L  RSS+  
Sbjct: 121 RRLSFFSYITPRMKQLFPNINWHKAKGLNFHFAKPEFKTKGGIVVRSIFTNLHKRSSNLR 180

Query: 181 NMESGSYTSPNEIIRCTDAYQSLYCQLLCGLLQREKVVGIGALFASTLVYVSKFLENHWV 240
           +M SG+ TSP++II CTD+YQSLYCQLLCGL Q + V  + ALFASTL++V KFLENHW+
Sbjct: 181 SMPSGNNTSPDDIILCTDSYQSLYCQLLCGLYQNDVVFRVSALFASTLIHVFKFLENHWI 240

Query: 241 LLANDIRTGTVNTKITDQLVRESVMNIL-KPDPQLADFIESECSKGTWEGIVTRLWPNTK 300
            LA DIRT T+N KIT   VRESVM I+ KP+P++AD IE+EC KG WEGI+TRLWPN K
Sbjct: 241 DLATDIRTRTINPKITSSSVRESVMKIVVKPNPEVADLIENECGKGRWEGIITRLWPNAK 300

Query: 301 FINAIVTGSMSQYIPQLNYYSNCLPLISLFYASSECYFGINLDPLSKPEEISYTFIPTMA 360
           +INA+VTGSMSQYIP LNYY+N LP++S  Y SSEC+ G+NLDPL  P+E+SYT IPTMA
Sbjct: 301 YINAVVTGSMSQYIPLLNYYTNNLPIVSDHYGSSECFLGLNLDPLCNPDEVSYTLIPTMA 360

Query: 361 YFEFLPVDRANANREVGQELVDLVDVKLGQEYEVIVTTYTGLYRYCIGDIVRVTGFKYKA 420
           YFEFLP+D  N   EV Q LVDLVDVKLG EYE+++TT+ GLYRYC+GD+VRVTGFK KA
Sbjct: 361 YFEFLPIDMLNPKGEVNQRLVDLVDVKLGHEYELVITTFAGLYRYCMGDVVRVTGFKNKA 420

Query: 421 PCFSFVCRKNVVLSLDFEKTDEAELHNAVKGAGELLNPFGATIADYTSYADNSTFPGHYV 480
           P FSFV RKNVVL++  EKTDEA LH AV+  G ++  FGATI DYT+YAD ST PGHYV
Sbjct: 421 PNFSFVRRKNVVLNISNEKTDEAGLHKAVEEGGRVMKQFGATIVDYTTYADISTIPGHYV 480

Query: 481 LYWEIHF----KEEISIIPSSIYEDCCLAVEESLNSLYRQARSHQKVINPLEIKMVEAGT 540
           LYWE+      ++ ++ IP S++EDCCLA+E SLN LYR ARSH+K INPLEIK+V+AGT
Sbjct: 481 LYWELTLDDLKEQNLNYIPCSVFEDCCLAIENSLNLLYRLARSHEKCINPLEIKIVKAGT 540

Query: 541 FDKLLERATYQGASISQYKTPRCVTSTQI--VELLDTKVVASYFSKKYPKWD 582
           F+KL++ A  +GASI+QYKTPRC+ S+QI  ++LL++ +V++YFSK YP  +
Sbjct: 541 FEKLMQLALNRGASITQYKTPRCLNSSQIHIIQLLESNLVSNYFSKNYPNLE 592

BLAST of MC02g_new0100 vs. TAIR 10
Match: AT5G54510.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 714.9 bits (1844), Expect = 5.3e-206
Identity = 352/592 (59.46%), Postives = 446/592 (75.34%), Query Frame = 0

Query: 6   DTTLDEKDKQVLQHLEDVTSNADEIQRRVLTEILSKNAHVEYLQQHGLDGSTDASTFKNL 65
           D +L EK+K  LQ +EDVT+NAD++QRRVL EILS+NA VEYL++HGL+G TD  TFK++
Sbjct: 14  DESLAEKNKNKLQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHGLEGRTDRETFKHI 73

Query: 66  VPIVSYEHLKPYIDRIANGDDSPILCAHPITEFLISSGTSGGEQKLFPMFEQELVRRLSI 125
           +P+V+YE ++P I+RIANGD S +LC++PI+EFL SSGTSGGE+KL P  E+EL RR  +
Sbjct: 74  MPVVTYEDIQPEINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLMPTIEEELDRRSLL 133

Query: 126 RNCMRPKIKELFPSLYNGKEMFFHFARAESVTKGGIVARSASTSLSRSSDFNNMESG--- 185
            + + P + +  P L  GK M+F F ++ES T GG+ AR   TS  +SS F N       
Sbjct: 134 YSLLMPVMDQFVPGLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKNRPYDPYT 193

Query: 186 SYTSPNEIIRCTDAYQSLYCQLLCGLLQREKVVGIGALFASTLVYVSKFLENHWVLLAND 245
           +YTSPN+ I C+D+YQS+Y Q+LCGL Q ++V+ +GA+FAS  +   KFLE HW  LA D
Sbjct: 194 NYTSPNQTILCSDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIKFLEKHWPELARD 253

Query: 246 IRTGTVNTKITDQLVRESVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAIV 305
           IRTGT++++ITD  VRE+V  ILKPDP+LADF+ESEC K +W+GI+TRLWPNTK+++ IV
Sbjct: 254 IRTGTLSSEITDSSVREAVGEILKPDPKLADFVESECRKTSWQGIITRLWPNTKYVDVIV 313

Query: 306 TGSMSQYIPQLNYYSNCLPLISLFYASSECYFGINLDPLSKPEEISYTFIPTMAYFEFLP 365
           TG+MSQYIP L+YYSN LPL+   YASSECYFG+NL PL KP E+SYT IP MAYFEFLP
Sbjct: 314 TGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPNMAYFEFLP 373

Query: 366 VDRANA-----------NREVGQELVDLVDVKLGQEYEVIVTTYTGLYRYCIGDIVRVTG 425
           V R +              +  QELVDLVDVKLGQEYE++VTTY GLYRY +GD++ V G
Sbjct: 374 VHRNSGVTSSISLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLYRYRVGDVLSVAG 433

Query: 426 FKYKAPCFSFVCRKNVVLSLDFEKTDEAELHNAVKGAGELLNPFGATIADYTSYADNSTF 485
           FK  AP FSF+CRKNVVLS+D +KTDE EL NAVK A   L PF A++++YTSYAD S+ 
Sbjct: 434 FKNNAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSI 493

Query: 486 PGHYVLYWEIHFKEEISIIPSSIYEDCCLAVEESLNSLYRQARSHQKVINPLEIKMVEAG 545
           PGHYVL+WE+      + IP S++EDCCL +EESLNS+YRQ R   K I PLEIKMVE+G
Sbjct: 494 PGHYVLFWELCLNGN-TPIPPSVFEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKMVESG 553

Query: 546 TFDKLLERATYQGASISQYKTPRCVTSTQIVELLDTKVVASYFSKKYPKWDP 584
           TFDKL++ A   GASI+QYKTPRCV    I+ELL+++VV SYFS K PKW P
Sbjct: 554 TFDKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWSP 604

BLAST of MC02g_new0100 vs. TAIR 10
Match: AT4G27260.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 710.7 bits (1833), Expect = 1.0e-204
Identity = 351/592 (59.29%), Postives = 446/592 (75.34%), Query Frame = 0

Query: 6   DTTLDEKDKQVLQHLEDVTSNADEIQRRVLTEILSKNAHVEYLQQHGLDGSTDASTFKNL 65
           D TLD+K+KQ LQ +E++TSNAD++QR+VL EIL++NA VEYL++H L+G TD  TFKN+
Sbjct: 14  DLTLDQKNKQKLQLIEELTSNADQVQRQVLEEILTRNADVEYLRRHDLNGRTDRETFKNI 73

Query: 66  VPIVSYEHLKPYIDRIANGDDSPILCAHPITEFLISSGTSGGEQKLFPMFEQELVRRLSI 125
           +P+++YE ++P I+RIANGD SPIL + PI+EFL SSGTSGGE+KL P  E+EL RR  +
Sbjct: 74  MPVITYEDIEPEINRIANGDKSPILSSKPISEFLTSSGTSGGERKLMPTIEEELDRRSLL 133

Query: 126 RNCMRPKIKELFPSLYNGKEMFFHFARAESVTKGGIVARSASTSLSRSSDFNNMESG--- 185
            + + P + +  P L NGK M+F F ++ES T GG+ AR   TS  +SS F         
Sbjct: 134 YSLLMPVMSQFVPGLENGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKERPYDPYT 193

Query: 186 SYTSPNEIIRCTDAYQSLYCQLLCGLLQREKVVGIGALFASTLVYVSKFLENHWVLLAND 245
           +YTSPNE I C+D+YQS+Y Q+LCGL Q ++V+ +GA+FAS  +   KFLE HW+ L  D
Sbjct: 194 NYTSPNETILCSDSYQSMYSQMLCGLCQHQEVLRVGAVFASGFIRAIKFLEKHWIELVRD 253

Query: 246 IRTGTVNTKITDQLVRESVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAIV 305
           IRTGT+++ ITD  VRE+V  ILKP P+LADF+E EC K +W+GI+TRLWPNTK+++ IV
Sbjct: 254 IRTGTLSSLITDPSVREAVAKILKPSPKLADFVEFECKKSSWQGIITRLWPNTKYVDVIV 313

Query: 306 TGSMSQYIPQLNYYSNCLPLISLFYASSECYFGINLDPLSKPEEISYTFIPTMAYFEFLP 365
           TG+MSQYIP L+YYSN LPL+   YASSECYFG+NL PL KP E+SYT IP+MAYFEFLP
Sbjct: 314 TGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPSMAYFEFLP 373

Query: 366 VDRANA-----------NREVGQELVDLVDVKLGQEYEVIVTTYTGLYRYCIGDIVRVTG 425
           V R N              +  QELVDLVDVKLGQEYE++VTTY GL RY +GD++RVTG
Sbjct: 374 VHRNNGVTNSINLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLCRYRVGDLLRVTG 433

Query: 426 FKYKAPCFSFVCRKNVVLSLDFEKTDEAELHNAVKGAGELLNPFGATIADYTSYADNSTF 485
           FK KAP FSF+CRKNVVLS+D +KTDE EL NAVK A   L PF A++++YTSYAD S+ 
Sbjct: 434 FKNKAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSI 493

Query: 486 PGHYVLYWEIHFKEEISIIPSSIYEDCCLAVEESLNSLYRQARSHQKVINPLEIKMVEAG 545
           PGHYVL+WE+   +  + IP S++EDCCLAVEES N++YRQ R   K I PLEIK+VE G
Sbjct: 494 PGHYVLFWELCL-DGNTPIPPSVFEDCCLAVEESFNTVYRQGRVSDKSIGPLEIKIVEPG 553

Query: 546 TFDKLLERATYQGASISQYKTPRCVTSTQIVELLDTKVVASYFSKKYPKWDP 584
           TFDKL++ A   GASI+QYKTPRCV    I+ELL+++VV SYFS K PKW P
Sbjct: 554 TFDKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWVP 604

BLAST of MC02g_new0100 vs. TAIR 10
Match: AT2G14960.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 605.9 bits (1561), Expect = 3.5e-173
Identity = 301/592 (50.84%), Postives = 408/592 (68.92%), Query Frame = 0

Query: 1   MSNALDTTLDEKDKQVLQHLEDVTSNADEIQRRVLTEILSKNAHVEYLQQHGLDGSTDAS 60
           +S+ L     EKD + L+ +E++T NAD +Q  +L EIL++NA  EYL++  L G+TD  
Sbjct: 7   LSSPLGPPACEKDAKALRFIEEMTRNADTVQENLLAEILARNADTEYLRRFNLCGATDRD 66

Query: 61  TFKNLVPIVSYEHLKPYIDRIANGDDSPILCAHPITEFLISSGTSGGEQKLFPMFEQELV 120
           TFK  +P+++YE L+P I RIA+GD SPIL AHPI+EFL SSGTS GE+KL P  ++EL 
Sbjct: 67  TFKTKIPVITYEDLQPEIQRIADGDRSPILSAHPISEFLTSSGTSAGERKLMPTIKEELD 126

Query: 121 RRLSIRNCMRPKIKELFPSLYNGKEMFFHFARAESVTKGGIVARSASTSLSRSSDFNNM- 180
           RR  + + + P +    P L  GK M+F F ++E+ T GG+ AR   TS  +S  F +  
Sbjct: 127 RRQLLYSLLMPVMNLYVPGLDKGKGMYFLFVKSETKTPGGLPARPVLTSYYKSEHFRSRP 186

Query: 181 --ESGSYTSPNEIIRCTDAYQSLYCQLLCGLLQREKVVGIGALFASTLVYVSKFLENHWV 240
                 YTSPNE I C D++QS+Y Q+LCGLL R  V+ +GA+FAS L+   +FL+ HW 
Sbjct: 187 YDPYNVYTSPNEAILCPDSFQSMYTQMLCGLLDRLSVLRVGAVFASGLLRAIRFLQLHWS 246

Query: 241 LLANDIRTGTVNTKITDQLVRESVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKF 300
             A+DI  G ++++ITD  +R+ +  ILKPDP LA+FI  EC    WE I+TR+WPNTK+
Sbjct: 247 RFAHDIELGCLDSEITDPSIRQCMSGILKPDPVLAEFIRRECKSDNWEKIITRIWPNTKY 306

Query: 301 INAIVTGSMSQYIPQLNYYSNCLPLISLFYASSECYFGINLDPLSKPEEISYTFIPTMAY 360
           ++ IVTG+M+QYIP L YYS  LP+    YASSECYFG+NL+P+SKP E+SYT +P MAY
Sbjct: 307 LDVIVTGAMAQYIPTLEYYSGGLPMACTMYASSECYFGLNLNPMSKPSEVSYTIMPNMAY 366

Query: 361 FEFLPVDRANANREVGQELVDLVDVKLGQEYEVIVTTYTGLYRYCIGDIVRVTGFKYKAP 420
           FEF+P+         G + V+LVDV +G+EYE++VTTY GL RY +GDI+RVTGF   AP
Sbjct: 367 FEFIPLG--------GTKAVELVDVNIGKEYELVVTTYAGLCRYRVGDILRVTGFHNSAP 426

Query: 421 CFSFVCRKNVVLSLDFEKTDEAELHNAVKGAGELLN-PFGATIADYTSYADNSTFPGHYV 480
            F FV RKNV+LS+D +KTDE+EL  AV+ A  +L+   G+ +A+YTSYAD ST PGHYV
Sbjct: 427 QFHFVRRKNVLLSIDSDKTDESELQKAVENASSILHEECGSRVAEYTSYADTSTIPGHYV 486

Query: 481 LYWEIHFKEEISIIPSSIYEDCCLAVEESLNSLYRQARSHQKVINPLEIKMVEAGTFDKL 540
           LYWE+  ++            CCL +EESLNS+YRQ+R     + PLEI++V  GTF++L
Sbjct: 487 LYWELLVRDGARQPSHETLTRCCLGMEESLNSVYRQSRVADNSVGPLEIRVVRNGTFEEL 546

Query: 541 LERATYQGASISQYKTPRCVTSTQIVELLDTKVVASYFSKKYPKWDPTCRHR 589
           ++ A  +GASI+QYK PRCV  T IVELLD++VV+++FS   P W P  R R
Sbjct: 547 MDYAISRGASINQYKVPRCVNFTPIVELLDSRVVSAHFSPSLPHWTPERRRR 590

BLAST of MC02g_new0100 vs. TAIR 10
Match: AT1G59500.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 602.8 bits (1553), Expect = 3.0e-172
Identity = 293/589 (49.75%), Postives = 412/589 (69.95%), Query Frame = 0

Query: 2   SNALDTTLDEKDKQVLQHLEDVTSNADEIQRRVLTEILSKNAHVEYLQQHGLDGSTDAST 61
           S     T  E + + L+ +E++T N D +Q +VL EILS+N++ EYL++  L+G+ D  +
Sbjct: 9   SGMASPTTSETEVKALKFIEEITRNPDSVQEKVLGEILSRNSNTEYLKRFDLNGAVDRKS 68

Query: 62  FKNLVPIVSYEHLKPYIDRIANGDDSPILCAHPITEFLISSGTSGGEQKLFPMFEQELVR 121
           FK+ VP+V YE LK  I RI+NGD SPIL +HPITEFL SSGTS GE+KL P  E+++ R
Sbjct: 69  FKSKVPVVIYEDLKTDIQRISNGDRSPILSSHPITEFLTSSGTSAGERKLMPTIEEDINR 128

Query: 122 RLSIRNCMRPKIKELFPSLYNGKEMFFHFARAESVTKGGIVARSASTSLSRSSDFNNMES 181
           R  + N + P +    P L  GK ++F F ++ES T GG+ AR A TS  +S  F   +S
Sbjct: 129 RQLLGNLLMPVMNLYVPGLDKGKGLYFLFVKSESTTSGGLPARPALTSYYKSDYFRTSDS 188

Query: 182 GS-YTSPNEIIRCTDAYQSLYCQLLCGLLQREKVVGIGALFASTLVYVSKFLENHWVLLA 241
            S YTSP E I C D+ QS+Y Q+LCGLL R +V  +GA+F S L+    FL+N+W  L+
Sbjct: 189 DSVYTSPKEAILCCDSSQSMYTQMLCGLLMRHEVNRLGAVFPSGLLRAISFLQNNWKELS 248

Query: 242 NDIRTGTVNTKITDQLVRESVMNIL-KPDPQLADFIESECSKGTWEGIVTRLWPNTKFIN 301
            DI TGT+++KI D  ++  + NIL KPD +LA+F+   CS+  WEGI+T++WPNTK+++
Sbjct: 249 QDISTGTLSSKIFDHAIKTRMSNILNKPDQELAEFLIGVCSQENWEGIITKIWPNTKYLD 308

Query: 302 AIVTGSMSQYIPQLNYYSNCLPLISLFYASSECYFGINLDPLSKPEEISYTFIPTMAYFE 361
            IVTG+M++YIP L YYS  LP+ S+ YASSE YFGINL+P+ KP E+SYT  P MAYFE
Sbjct: 309 VIVTGAMAEYIPMLEYYSGGLPMASMIYASSESYFGINLNPMCKPSEVSYTIFPNMAYFE 368

Query: 362 FLPVDRANANREVGQELVDLVDVKLGQEYEVIVTTYTGLYRYCIGDIVRVTGFKYKAPCF 421
           FLP +           LV+L DV++G+EYE+++TTY GLYRY +GDI+RVTGF   AP F
Sbjct: 369 FLPHNHDGDGGVEATSLVELADVEVGKEYELVITTYAGLYRYRVGDILRVTGFHNSAPQF 428

Query: 422 SFVCRKNVVLSLDFEKTDEAELHNAVKGAGELLNPFGATIADYTSYADNSTFPGHYVLYW 481
            F+ R+NV+LS++ +KTDEA+L  AV+ A  LL   G  + +YTSYAD  T PGHYV+YW
Sbjct: 429 KFIRRENVLLSIESDKTDEADLQKAVENASRLLAEQGTRVIEYTSYADTKTIPGHYVIYW 488

Query: 482 EIHFKEEISIIPS-SIYEDCCLAVEESLNSLYRQARSHQKVINPLEIKMVEAGTFDKLLE 541
           E+  +++ + +PS  +   CCL +EESLN++YRQ+R   K I PLEI++V+ GTF++L++
Sbjct: 489 ELLSRDQSNALPSDEVMAKCCLEMEESLNAVYRQSRVSDKSIGPLEIRVVQNGTFEELMD 548

Query: 542 RATYQGASISQYKTPRCVTSTQIVELLDTKVVASYFSKKYPKWDPTCRH 588
            +  +G+SI+QYK PRCV+ T I++LLD++VV+++FS   P W P  RH
Sbjct: 549 FSISRGSSINQYKVPRCVSLTPIMKLLDSRVVSAHFSPSLPHWSPERRH 597

BLAST of MC02g_new0100 vs. TAIR 10
Match: AT4G37390.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 596.3 bits (1536), Expect = 2.8e-170
Identity = 298/595 (50.08%), Postives = 412/595 (69.24%), Query Frame = 0

Query: 2   SNALDTTLDEKDKQVLQHLEDVTSNADEIQRRVLTEILSKNAHVEYLQQHGLDGSTDAST 61
           S  +  T  EKD + L+ +E++T N D +Q +VL EIL++N++ EYL++  LDG  D  T
Sbjct: 9   SRMVSATTSEKDVKALKFIEEMTRNPDSVQEKVLGEILTRNSNTEYLKRFDLDGVVDRKT 68

Query: 62  FKNLVPIVSYEHLKPYIDRIANGDDSPILCAHPITEFLISSGTSGGEQKLFPMFEQELVR 121
           FK+ VP+V+YE LKP I RI+NGD SPIL +HPITEFL SSGTS GE+KL P  E++L R
Sbjct: 69  FKSKVPVVTYEDLKPEIQRISNGDCSPILSSHPITEFLTSSGTSAGERKLMPTIEEDLDR 128

Query: 122 RLSIRNCMRPKIKELFPSLYNGKEMFFHFARAESVTKGGIVARSASTSLSRSSDFNNM-- 181
           R  + + + P +    P L  GK ++F F ++ES T GG+ AR   TS  +S  F     
Sbjct: 129 RQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSESKTSGGLPARPVLTSYYKSDHFKRRPY 188

Query: 182 -ESGSYTSPNEIIRCTDAYQSLYCQLLCGLLQREKVVGIGALFASTLVYVSKFLENHWVL 241
                YTSPNE I C+D+ QS+Y Q+LCGLL R +V+ +GA+FAS L+    FL+N+W  
Sbjct: 189 DPYNVYTSPNEAILCSDSSQSMYAQMLCGLLMRHEVLRLGAVFASGLLRAISFLQNNWKE 248

Query: 242 LANDIRTGTVNTKITDQLVRESVMNIL-KPDPQLADFIESECSKGTWEGIVTRLWPNTKF 301
           LA DI TGT++++I D  ++  +  IL KPD +LA+F+   CS+  WEGI+T++WPNTK+
Sbjct: 249 LARDISTGTLSSRIFDPAIKNRMSKILTKPDQELAEFLVGVCSQENWEGIITKIWPNTKY 308

Query: 302 INAIVTGSMSQYIPQLNYYSNCLPLISLFYASSECYFGINLDPLSKPEEISYTFIPTMAY 361
           ++ IVTG+M+QYIP L YYS  LP+    YASSE YFGINL P+ KP E+SYT +P MAY
Sbjct: 309 LDVIVTGAMAQYIPTLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSYTIMPNMAY 368

Query: 362 FEFLPVDR-----ANANREVGQELVDLVDVKLGQEYEVIVTTYTGLYRYCIGDIVRVTGF 421
           FEFLP +      A A+ +    LV+L +V++G+EYE+++TTY GLYRY +GDI+RVTGF
Sbjct: 369 FEFLPHNHDGDGAAEASLD-ETSLVELANVEVGKEYELVITTYAGLYRYRVGDILRVTGF 428

Query: 422 KYKAPCFSFVCRKNVVLSLDFEKTDEAELHNAVKGAGELLNPFGATIADYTSYADNSTFP 481
              AP F F+ RKNV+LS++ +KTDEAEL  AV+ A  L    G  + +YTSYA+  T P
Sbjct: 429 HNSAPQFKFIRRKNVLLSVESDKTDEAELQKAVENASRLFAEQGTRVIEYTSYAETKTIP 488

Query: 482 GHYVLYWEIHFKEEISIIPS-SIYEDCCLAVEESLNSLYRQARSHQKVINPLEIKMVEAG 541
           GHYV+YWE+  +++ + + S  +   CCL +EESLNS+YRQ+R   K I PLEI++V  G
Sbjct: 489 GHYVIYWELLGRDQSNALMSEEVMAKCCLEMEESLNSVYRQSRVADKSIGPLEIRVVRNG 548

Query: 542 TFDKLLERATYQGASISQYKTPRCVTSTQIVELLDTKVVASYFSKKYPKWDPTCR 587
           TF++L++ A  +GASI+QYK PRCV+ T I+ELLD++VV+++FS   P W P  R
Sbjct: 549 TFEELMDYAISRGASINQYKVPRCVSFTPIMELLDSRVVSAHFSPSLPHWSPERR 602