MC02g1308 (gene) Bitter gourd (Dali-11) v1

BLAST of MC02g1308 vs. ExPASy Swiss-Prot
Match: Q9M3H5 (Probable cadmium/zinc-transporting ATPase HMA1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=HMA1 PE=2 SV=2)

HSP 1 Score: 1084.3 bits (2803), Expect = 0.0e+00
Identity = 553/736 (75.14%), Postives = 630/736 (85.60%), Query Frame = 0

Query: 65  HHHGHHHGCQHHCDGDGVEPTGAQKAFIRFAKAIRWTELANYLREHLQLCCGSAALFLTA 124
           HHH H HGC   C  +    +  QK    FAKAI W  LANYLREHL LCC +AA+FL A
Sbjct: 83  HHHHHQHGC---CSVELKAESKPQKMLFGFAKAIGWVRLANYLREHLHLCCSAAAMFLAA 142

Query: 125 AACPYLVPKPAVKPLQNVLVAVAFPLVGVSASLDALTDISGGKVNIHVLMALAAFASIFM 184
           A CPYL P+P +K LQN  + V FPLVGVSASLDAL DI+GGKVNIHVLMALAAFAS+FM
Sbjct: 143 AVCPYLAPEPYIKSLQNAFMIVGFPLVGVSASLDALMDIAGGKVNIHVLMALAAFASVFM 202

Query: 185 GNPLEGGLLLVMFNLAHIAEEYFTKQSMIDVKELKESHPDFALVLDMNDDNLPDTSDLEH 244
           GN LEGGLLL MFNLAHIAEE+FT +SM+DVKELKES+PD AL++++++ N+P+ SDL +
Sbjct: 203 GNALEGGLLLAMFNLAHIAEEFFTSRSMVDVKELKESNPDSALLIEVHNGNVPNISDLSY 262

Query: 245 RRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHLTGEVKPLEIKVGERVPGGAR 304
           + VPV  VEVGSY+LVG GE VPVDCEVYQG ATIT+EHLTGEVKPLE K G+RVPGGAR
Sbjct: 263 KSVPVHSVEVGSYVLVGTGEIVPVDCEVYQGSATITIEHLTGEVKPLEAKAGDRVPGGAR 322

Query: 305 NLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWLDEFGENYSKVVVVLSVAVAL 364
           NLDGR+IVKATK W +STL++IVQLTEEA  NKPKLQRWLDEFGENYSKVVVVLS+A+A 
Sbjct: 323 NLDGRMIVKATKAWNDSTLNKIVQLTEEAHSNKPKLQRWLDEFGENYSKVVVVLSLAIAF 382

Query: 365 IGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPLAYAIAISSCARKGILLKGGH 424
           +GP LFKWPF+ T   RGSVYRALGLMVAASPCALA APLAYA AISSCARKGILLKG  
Sbjct: 383 LGPFLFKWPFLSTAACRGSVYRALGLMVAASPCALAVAPLAYATAISSCARKGILLKGAQ 442

Query: 425 VLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIFGHKVGGEKTHLASCCIPSCEKEALAVA 484
           VLDA+ASCHT+AFDKTGTLTTGGL  KAIEPI+GH+ GG  + + +CCIP+CEKEALAVA
Sbjct: 443 VLDALASCHTIAFDKTGTLTTGGLTCKAIEPIYGHQ-GGTNSSVITCCIPNCEKEALAVA 502

Query: 485 AAMEKGTTHPIGRAVVDHSAGKDLPSISVESFEYFPGRGLIATLHGIKS-GIGGKLRKAS 544
           AAMEKGTTHPIGRAVVDHS GKDLPSI VESFEYFPGRGL AT++G+K+     +LRKAS
Sbjct: 503 AAMEKGTTHPIGRAVVDHSVGKDLPSIFVESFEYFPGRGLTATVNGVKTVAEESRLRKAS 562

Query: 545 LGSVDFITSFCKSENESRKIKEAVRASSYGSEFVHAALSVDQKVTLIHLEDQPRPGMLNA 604
           LGS++FITS  KSE+ES++IK+AV ASSYG +FVHAALSVDQKVTLIHLEDQPRPG+   
Sbjct: 563 LGSIEFITSLFKSEDESKQIKDAVNASSYGKDFVHAALSVDQKVTLIHLEDQPRPGVSGV 622

Query: 605 ISELQDHAKVRVMMLTGDHDSSAWKVANAVGINEVYYSLKPEDKLAHVKEISREMGGGLI 664
           I+EL+  A++RVMMLTGDHDSSAW+VANAVGI EVY +LKPEDKL HVK I+RE GGGLI
Sbjct: 623 IAELKSWARLRVMMLTGDHDSSAWRVANAVGITEVYCNLKPEDKLNHVKNIAREAGGGLI 682

Query: 665 MVGEGINDAPALAAATVGIVLARRASATATAVADVLLLQDNISCVPFCIAKSRQTTSLVK 724
           MVGEGINDAPALAAATVGIVLA+RASATA AVAD+LLL+DNI+ VPFC+AKSRQTTSLVK
Sbjct: 683 MVGEGINDAPALAAATVGIVLAQRASATAIAVADILLLRDNITGVPFCVAKSRQTTSLVK 742

Query: 725 QNVTLALSSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLNSIRALNQPSWSWKQDFQNL 784
           QNV LAL+SI LA+LPSVLGF+PLWLTVLLHEGGTLLVCLNS+R LN PSWSWKQD  +L
Sbjct: 743 QNVALALTSIFLAALPSVLGFVPLWLTVLLHEGGTLLVCLNSVRGLNDPSWSWKQDIVHL 802

Query: 785 VRDARSRVLATRQTSS 800
           +   RS+   +  ++S
Sbjct: 803 INKLRSQEPTSSSSNS 814

BLAST of MC02g1308 vs. ExPASy Swiss-Prot
Match: Q60048 (Probable cadmium-transporting ATPase OS=Listeria monocytogenes OX=1639 GN=cadA PE=1 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 3.2e-73
Identity = 187/635 (29.45%), Postives = 327/635 (51.50%), Query Frame = 0

Query: 139 LQNVLVAVAFPLVGVSASLDALTDISGGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFN 198
           L N L   A  + G S   +   ++   +  +  LM +A   + F+G   EG +++++F 
Sbjct: 114 LTNALFIFAIFIGGYSLFKEGFKNLLKFEFTMETLMTIAIIGAAFIGEWAEGSIVVILFA 173

Query: 199 LAHIAEEYFTKQSMIDVKELKESHPDFALVLDMNDDNLPDTSDLEHRRVPVRDVEVGSYI 258
           ++   E Y   ++   ++ L +  P  ALV     D          R V V D+++G  +
Sbjct: 174 VSEALERYSMDKARQSIRSLMDIAPKEALVRRSGTD----------RMVHVDDIQIGDIM 233

Query: 259 LVGAGESVPVDCEVYQGCATITVEHLTGEVKPLEIKVGERVPGGARNLDGRIIVKATKTW 318
           ++  G+ + +D  V +G + +    +TGE  P+E  + + V  G  N +G + V  TK  
Sbjct: 234 IIKPGQKIAMDGHVVKGYSAVNQAAITGESIPVEKNIDDSVFAGTLNEEGLLEVAVTKRV 293

Query: 319 EESTLSRIVQLTEEAQLNKPKLQRWLDEFGENYSKVVVVLSVAVALIGPLLFKWPFICTP 378
           E++T+S+I+ L EEAQ  +   Q ++D F + Y+  ++V++  +A + PLLF        
Sbjct: 294 EDTTISKIIHLVEEAQGERAPAQAFVDTFAKYYTPAIIVIAALIATVPPLLF------GG 353

Query: 379 GFRGSVYRALGLMVAASPCAL-AAAPLAYAIAISSCARKGILLKGGHVLDAMASCHTVAF 438
            +   VY+ L ++V   PCAL  + P+A   AI + A+ G+L+KGG  L+ +     +AF
Sbjct: 354 NWETWVYQGLSVLVVGCPCALVVSTPVAIVTAIGNAAKNGVLVKGGVYLEEIGGLKAIAF 413

Query: 439 DKTGTLTTG-GLVFKAIEPIFGHKVGGEKTHLASCCIPSCEKEALAVAAAMEKGTTHPIG 498
           DKTGTLT G  +V   IE      +   K ++              + AA+E+ + HP+ 
Sbjct: 414 DKTGTLTKGVPVVTDYIELTEATNIQHNKNYI--------------IMAALEQLSQHPLA 473

Query: 499 RAVVDHSAGK--DLPSISVESFEYFPGRGLIATLHGIKSGIGGKLRKASLGSVDFITSFC 558
            A++ +   +  DL SI+V  F    G+G+  T+ G    +G  +    L +  F  S  
Sbjct: 474 SAIIKYGETREMDLTSINVNDFTSITGKGIRGTVDGNTYYVGSPVLFKELLASQFTDSIH 533

Query: 559 KSENESRKIKEAVRASSYGSEFVHAALSVDQKVTLIHLEDQPRPGMLNAISELQDHAKVR 618
           +  ++ +   +   A  +G+          + ++++ + D+ R    + I  L +    +
Sbjct: 534 RQVSDLQ--LKGKTAMLFGTN--------QKLISIVAVADEVRSSSQHVIKRLHELGIEK 593

Query: 619 VMMLTGDHDSSAWKVANAVGINEVYYSLKPEDKLAHVKEISREMGGGLIMVGEGINDAPA 678
            +MLTGD+ ++A  +   VG++E+   L P+DKL ++K++     G + MVG+GINDAPA
Sbjct: 594 TIMLTGDNQATAQAIGQQVGVSEIEGELMPQDKLDYIKQLKINF-GKVAMVGDGINDAPA 653

Query: 679 LAAATVGIVLARRASATATAVADVLLLQDNISCVPFCIAKSRQTTSLVKQNVTLALSSIL 738
           LAAATVGI +    + TA   ADV L+ D++  +PF +  SR+T  ++KQN+T +L   L
Sbjct: 654 LAAATVGIAMGGAGTDTAIETADVALMGDDLQKLPFTVKLSRKTLQIIKQNITFSLVIKL 707

Query: 739 LASLPSVLGFLPLWLTVLLHEGGTLLVCLNSIRAL 770
           +A L  + G+L LW+ ++   G TLLV LN +R +
Sbjct: 714 IALLLVIPGWLTLWIAIMADMGATLLVTLNGLRLM 707

BLAST of MC02g1308 vs. ExPASy Swiss-Prot
Match: P58414 (Probable cadmium-transporting ATPase OS=Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e) OX=169963 GN=cadA PE=3 SV=1)

HSP 1 Score: 275.8 bits (704), Expect = 1.6e-72
Identity = 184/632 (29.11%), Postives = 328/632 (51.90%), Query Frame = 0

Query: 142 VLVAVAFPLVGVSASLDALTDISGGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNLAH 201
           +L  +A  + G +   +   ++      +  LM +A   +  +G   EG +++++F  + 
Sbjct: 117 ILYVIAIVVGGFNLFKEGFANLIKLDFTMESLMTIAIIGASIIGEWAEGSIVVILFAFSE 176

Query: 202 IAEEYFTKQSMIDVKELKESHPDFALVLDMNDDNLPDTSDLEHRRVPVRDVEVGSYILVG 261
           + E Y   ++   ++ L +  P  AL+            D   + + V D+++G  +++ 
Sbjct: 177 VLERYSMDKARQSIRSLMDIAPKEALI----------RRDDVEQMIAVSDIQIGDIMIIK 236

Query: 262 AGESVPVDCEVYQGCATITVEHLTGEVKPLEIKVGERVPGGARNLDGRIIVKATKTWEES 321
            G+ + +D  V +G + I    +TGE  P+E KV + V  G  N +G + VK TK  E++
Sbjct: 237 PGQKIAMDGVVIKGYSAINQSAITGESIPVEKKVDDEVFAGTLNEEGLLEVKVTKHVEDT 296

Query: 322 TLSRIVQLTEEAQLNKPKLQRWLDEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFR 381
           T+S+I+ L EEAQ  +   Q ++D+F + Y+  ++++++ V ++ PL F   +       
Sbjct: 297 TISKIIHLVEEAQGERAPAQAFVDKFAKYYTPTIMLIALLVVVVPPLFFGGDWDTW---- 356

Query: 382 GSVYRALGLMVAASPCALA-AAPLAYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKT 441
             VY+ L L+V   PC+L  + P++   AI + A+ G+L+KGG  L+ +     +AFDKT
Sbjct: 357 --VYQGLSLLVVGCPCSLVISTPVSIVSAIGNSAKNGVLVKGGIYLEEIGGLQAIAFDKT 416

Query: 442 GTLTTGGLVFKAIEPIFGHKVGGEKTHLASCCIPSCEKEALAVAAAMEKGTTHPIGRAVV 501
           GTLT G  V     P   H                 E+ +L++  A+E  + HP+  A++
Sbjct: 417 GTLTKGKPVVTDFIPYSEH---------------MDEQNSLSIITALETMSQHPLASAII 476

Query: 502 DHSA--GKDLPSISVESFEYFPGRGLIATLHGIKSGIG-GKLRKASLGSVDFITSFCKSE 561
             +     D  SI +++F    G+G+   ++GI   IG  KL ++SL     I+   +S 
Sbjct: 477 SKAMIDNVDYKSIEIDNFSSITGKGVKGEVNGITYYIGSSKLFESSLEKSQSISQTYQSL 536

Query: 562 NESRKIKEAVRASSYGSEFVHAALSVDQKVTLIHLEDQPRPGMLNAISELQDHAKVRVMM 621
                 K+   A  +G+E           + +I + D+ R      I++L        +M
Sbjct: 537 Q-----KQGKTAMLFGTE--------SNILAIIAVADEVRESSKEVIAQLHKLGIAHTIM 596

Query: 622 LTGDHDSSAWKVANAVGINEVYYSLKPEDKLAHVKEISREMGGGLIMVGEGINDAPALAA 681
           LTGD++ +A  +   +G++++   L PEDKL ++KE+ ++  G + M+G+G+NDAPALAA
Sbjct: 597 LTGDNNDTAQFIGKEIGVSDIKAELMPEDKLTYIKEL-KQTYGKVAMIGDGVNDAPALAA 656

Query: 682 ATVGIVLARRASATATAVADVLLLQDNISCVPFCIAKSRQTTSLVKQNVTLALSSILLAS 741
           +TVGI +    + TA   ADV L+ D++  +PF +  SR+T  ++KQN+T +L   LLA 
Sbjct: 657 STVGIAMGGAGTDTALETADVALMGDDLKKLPFIVNLSRKTLKIIKQNITFSLGIKLLAL 703

Query: 742 LPSVLGFLPLWLTVLLHEGGTLLVCLNSIRAL 770
           L  + G+L LW+ ++   G TLLV LN +R +
Sbjct: 717 LLVLPGWLTLWIAIVADMGATLLVTLNGLRLM 703

BLAST of MC02g1308 vs. ExPASy Swiss-Prot
Match: Q6GIX1 (Probable cadmium-transporting ATPase OS=Staphylococcus aureus (strain MRSA252) OX=282458 GN=cadA PE=3 SV=1)

HSP 1 Score: 271.2 bits (692), Expect = 3.9e-71
Identity = 178/605 (29.42%), Postives = 316/605 (52.23%), Query Frame = 0

Query: 169 NIHVLMALAAFASIFMGNPLEGGLLLVMFNLAHIAEEYFTKQSMIDVKELKESHPDFALV 228
           ++  LM +A   +  +G   E  +++V+F ++   E +   ++   ++ L +  P  ALV
Sbjct: 160 DMKTLMTVAVIGAAIIGEWAEASIVVVLFAISEALERFSMDRARQSIRSLMDIAPKEALV 219

Query: 229 LDMNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHLTGEV 288
           +    + +          + V D+ VG  ++V  GE + +D  +  G + +    +TGE 
Sbjct: 220 MRNGQEIM----------IHVDDIAVGDIMIVKPGEKIAMDGIIINGVSAVNQAAITGES 279

Query: 289 KPLEIKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWLDEFG 348
            P+   V + V  G  N +G + VK TK  E++T+S+I+ L EEAQ  +   Q ++D+F 
Sbjct: 280 VPVAKTVDDEVFAGTLNEEGLLEVKITKYVEDTTISKIIHLVEEAQGERAPAQAFVDKFA 339

Query: 349 ENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALA-AAPLAYA 408
           + Y+ +++V++  VA++ PL F         +   VY+ L ++V   PCAL  + P++  
Sbjct: 340 KYYTPIIMVIAALVAVVPPLFF------GGSWDTWVYQGLAVLVVGCPCALVISTPISIV 399

Query: 409 IAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIFGHKVGGEKTH 468
            AI + A+KG+L+KGG  L+ + +   +AFDKTGTLT      K +  +   KV  ++  
Sbjct: 400 SAIGNAAKKGVLIKGGVYLEELGAIKAIAFDKTGTLT------KGVPVVTDFKVLNDQVE 459

Query: 469 LASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSAGKDL--PSISVESFEYFPGRGLI 528
                    EKE  ++  A+E  + HP+  A++  +   ++    + VE F    GRG+ 
Sbjct: 460 ---------EKELFSIITALEYRSQHPLASAIMKKAEQDNITYSDVRVEDFTSITGRGIQ 519

Query: 529 ATLHGIKSGIGGKLRKASLGSVDFITSFCKSENESRKIKEAVRASSYGSEFVHAALSVDQ 588
             + G    IG       L   DF   F   EN+ + ++   + +          +  DQ
Sbjct: 520 GNIDGTTYYIGSPRLFKELNVSDFSLEF---ENKVKVLQNQGKTA--------MIIGTDQ 579

Query: 589 KVT-LIHLEDQPRPGMLNAISELQDHAKVRVMMLTGDHDSSAWKVANAVGINEVYYSLKP 648
            +  +I + D+ R    N I +L      + +MLTGD+  +A  +   VG++++   L P
Sbjct: 580 TILGVIAVADEVRETSKNVIQKLHQLGIKQTIMLTGDNQGTAEAIGAHVGVSDIQSELMP 639

Query: 649 EDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLARRASATATAVADVLLLQDN 708
           +DKL ++K++  E  G + M+G+G+NDAPALAA+TVGI +    + TA   AD+ L+ D+
Sbjct: 640 QDKLDYIKKMKAE-HGNVAMIGDGVNDAPALAASTVGIAMGGAGTDTAIETADIALMGDD 699

Query: 709 ISCVPFCIAKSRQTTSLVKQNVTLALSSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLN 768
           +S +PF +  SR+T +++K N+T A+   ++A L  + G+L LW+ +L   G T+LV LN
Sbjct: 700 LSKLPFAVRLSRKTLNIIKANITFAIGIKIIALLLVIPGWLTLWIAILSDMGATILVALN 721

Query: 769 SIRAL 770
           S+R +
Sbjct: 760 SLRLM 721

BLAST of MC02g1308 vs. ExPASy Swiss-Prot
Match: P20021 (Cadmium-transporting ATPase OS=Staphylococcus aureus OX=1280 GN=cadA PE=1 SV=1)

HSP 1 Score: 268.9 bits (686), Expect = 1.9e-70
Identity = 182/605 (30.08%), Postives = 316/605 (52.23%), Query Frame = 0

Query: 169 NIHVLMALAAFASIFMGNPLEGGLLLVMFNLAHIAEEYFTKQSMIDVKELKESHPDFALV 228
           ++  LM +A   +  +G   E  +++++F ++   E +   +S   ++ L +  P  ALV
Sbjct: 161 DMKTLMTVAVIGATIIGKWAEASIVVILFAISEALERFSMDRSRQSIRSLMDIAPKEALV 220

Query: 229 LDMNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHLTGEV 288
                + +          + V D+ VG  ++V  GE + +D  +  G + +    +TGE 
Sbjct: 221 RRNGQEII----------IHVDDIAVGDIMIVKPGEKIAMDGIIVNGLSAVNQAAITGES 280

Query: 289 KPLEIKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWLDEFG 348
            P+   V + V  G  N +G I VK TK  E++T+++I+ L EEAQ  +   Q ++D+F 
Sbjct: 281 VPVSKAVDDEVFAGTLNEEGLIEVKITKYVEDTTITKIIHLVEEAQGERAPAQAFVDKFA 340

Query: 349 ENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALA-AAPLAYA 408
           + Y+ +++V++  VA++ PL F         +   VY+ L ++V   PCAL  + P++  
Sbjct: 341 KYYTPIIMVIAALVAVVPPLFF------GGSWDTWVYQGLAVLVVGCPCALVISTPISIV 400

Query: 409 IAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIFGHKVGGEKTH 468
            AI + A+KG+L+KGG  L+ + +  TVAFDKTGTLT G  V    E +   +V      
Sbjct: 401 SAIGNAAKKGVLVKGGVYLEKLGAIKTVAFDKTGTLTKGVPVVTDFE-VLNDQVE----- 460

Query: 469 LASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSAGKDLP--SISVESFEYFPGRGLI 528
                    EKE  ++  A+E  + HP+  A++  +   ++P  ++ VE F    GRG+ 
Sbjct: 461 ---------EKELFSIITALEYRSQHPLASAIMKKAEQDNIPYSNVQVEEFTSITGRGIK 520

Query: 529 ATLHGIKSGIGGKLRKASLGSVDFITSFCKSENESRKIK-EAVRASSYGSEFVHAALSVD 588
             ++G    IG       L   DF   F   EN  + ++ +   A   G+E         
Sbjct: 521 GIVNGTTYYIGSPKLFKELNVSDFSLGF---ENNVKILQNQGKTAMIIGTE--------K 580

Query: 589 QKVTLIHLEDQPRPGMLNAISELQDHAKVRVMMLTGDHDSSAWKVANAVGINEVYYSLKP 648
             + +I + D+ R    N I +L      + +MLTGD+  +A  +   VG++++   L P
Sbjct: 581 TILGVIAVADEVRETSKNVIQKLHQLGIKQTIMLTGDNQGTANAIGTHVGVSDIQSELMP 640

Query: 649 EDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLARRASATATAVADVLLLQDN 708
           +DKL ++K++  E    + M+G+G+NDAPALAA+TVGI +    + TA   AD+ L+ D+
Sbjct: 641 QDKLDYIKKMQSEY-DNVAMIGDGVNDAPALAASTVGIAMGGAGTDTAIETADIALMGDD 700

Query: 709 ISCVPFCIAKSRQTTSLVKQNVTLALSSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLN 768
           +S +PF +  SR+T +++K N+T A+   ++A L  + G+L LW+ +L   G T+LV LN
Sbjct: 701 LSKLPFAVRLSRKTLNIIKANITFAIGIKIIALLLVIPGWLTLWIAILSDMGATILVALN 722

Query: 769 SIRAL 770
           S+R +
Sbjct: 761 SLRLM 722

BLAST of MC02g1308 vs. NCBI nr
Match: XP_022150849.1 (probable cadmium/zinc-transporting ATPase HMA1, chloroplastic [Momordica charantia] >XP_022150850.1 probable cadmium/zinc-transporting ATPase HMA1, chloroplastic [Momordica charantia])

HSP 1 Score: 1575 bits (4078), Expect = 0.0
Identity = 807/807 (100.00%), Postives = 807/807 (100.00%), Query Frame = 0

Query: 1   MDALFVHHSYIRRTTNDGAHSTAIPVPRFSLRSSLIGIKLLHLHSKNFPYPRLRRIPCAP 60
           MDALFVHHSYIRRTTNDGAHSTAIPVPRFSLRSSLIGIKLLHLHSKNFPYPRLRRIPCAP
Sbjct: 1   MDALFVHHSYIRRTTNDGAHSTAIPVPRFSLRSSLIGIKLLHLHSKNFPYPRLRRIPCAP 60

Query: 61  EGAGHHHGHHHGCQHHCDGDGVEPTGAQKAFIRFAKAIRWTELANYLREHLQLCCGSAAL 120
           EGAGHHHGHHHGCQHHCDGDGVEPTGAQKAFIRFAKAIRWTELANYLREHLQLCCGSAAL
Sbjct: 61  EGAGHHHGHHHGCQHHCDGDGVEPTGAQKAFIRFAKAIRWTELANYLREHLQLCCGSAAL 120

Query: 121 FLTAAACPYLVPKPAVKPLQNVLVAVAFPLVGVSASLDALTDISGGKVNIHVLMALAAFA 180
           FLTAAACPYLVPKPAVKPLQNVLVAVAFPLVGVSASLDALTDISGGKVNIHVLMALAAFA
Sbjct: 121 FLTAAACPYLVPKPAVKPLQNVLVAVAFPLVGVSASLDALTDISGGKVNIHVLMALAAFA 180

Query: 181 SIFMGNPLEGGLLLVMFNLAHIAEEYFTKQSMIDVKELKESHPDFALVLDMNDDNLPDTS 240
           SIFMGNPLEGGLLLVMFNLAHIAEEYFTKQSMIDVKELKESHPDFALVLDMNDDNLPDTS
Sbjct: 181 SIFMGNPLEGGLLLVMFNLAHIAEEYFTKQSMIDVKELKESHPDFALVLDMNDDNLPDTS 240

Query: 241 DLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHLTGEVKPLEIKVGERVP 300
           DLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHLTGEVKPLEIKVGERVP
Sbjct: 241 DLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHLTGEVKPLEIKVGERVP 300

Query: 301 GGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWLDEFGENYSKVVVVLSV 360
           GGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWLDEFGENYSKVVVVLSV
Sbjct: 301 GGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWLDEFGENYSKVVVVLSV 360

Query: 361 AVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPLAYAIAISSCARKGILL 420
           AVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPLAYAIAISSCARKGILL
Sbjct: 361 AVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPLAYAIAISSCARKGILL 420

Query: 421 KGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIFGHKVGGEKTHLASCCIPSCEKEA 480
           KGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIFGHKVGGEKTHLASCCIPSCEKEA
Sbjct: 421 KGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIFGHKVGGEKTHLASCCIPSCEKEA 480

Query: 481 LAVAAAMEKGTTHPIGRAVVDHSAGKDLPSISVESFEYFPGRGLIATLHGIKSGIGGKLR 540
           LAVAAAMEKGTTHPIGRAVVDHSAGKDLPSISVESFEYFPGRGLIATLHGIKSGIGGKLR
Sbjct: 481 LAVAAAMEKGTTHPIGRAVVDHSAGKDLPSISVESFEYFPGRGLIATLHGIKSGIGGKLR 540

Query: 541 KASLGSVDFITSFCKSENESRKIKEAVRASSYGSEFVHAALSVDQKVTLIHLEDQPRPGM 600
           KASLGSVDFITSFCKSENESRKIKEAVRASSYGSEFVHAALSVDQKVTLIHLEDQPRPGM
Sbjct: 541 KASLGSVDFITSFCKSENESRKIKEAVRASSYGSEFVHAALSVDQKVTLIHLEDQPRPGM 600

Query: 601 LNAISELQDHAKVRVMMLTGDHDSSAWKVANAVGINEVYYSLKPEDKLAHVKEISREMGG 660
           LNAISELQDHAKVRVMMLTGDHDSSAWKVANAVGINEVYYSLKPEDKLAHVKEISREMGG
Sbjct: 601 LNAISELQDHAKVRVMMLTGDHDSSAWKVANAVGINEVYYSLKPEDKLAHVKEISREMGG 660

Query: 661 GLIMVGEGINDAPALAAATVGIVLARRASATATAVADVLLLQDNISCVPFCIAKSRQTTS 720
           GLIMVGEGINDAPALAAATVGIVLARRASATATAVADVLLLQDNISCVPFCIAKSRQTTS
Sbjct: 661 GLIMVGEGINDAPALAAATVGIVLARRASATATAVADVLLLQDNISCVPFCIAKSRQTTS 720

Query: 721 LVKQNVTLALSSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLNSIRALNQPSWSWKQDF 780
           LVKQNVTLALSSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLNSIRALNQPSWSWKQDF
Sbjct: 721 LVKQNVTLALSSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLNSIRALNQPSWSWKQDF 780

Query: 781 QNLVRDARSRVLATRQTSSGTIQTASL 807
           QNLVRDARSRVLATRQTSSGTIQTASL
Sbjct: 781 QNLVRDARSRVLATRQTSSGTIQTASL 807

BLAST of MC02g1308 vs. NCBI nr
Match: XP_038901378.1 (probable cadmium/zinc-transporting ATPase HMA1, chloroplastic isoform X2 [Benincasa hispida])

HSP 1 Score: 1397 bits (3616), Expect = 0.0
Identity = 724/817 (88.62%), Postives = 757/817 (92.66%), Query Frame = 0

Query: 1   MDALFVH-------HSYIRRTTNDGAHSTAIPVPRFSLRSSLIGIKLLHLHSKNFPYPRL 60
           MD LFV        H  + R TN GA S   P+ RFS RSSLI +    +  K   YPR 
Sbjct: 1   MDTLFVPISFSESPHPLLTRNTNAGAQSRIGPLSRFSPRSSLIRLNRFPIPCKKISYPRF 60

Query: 61  RRIPCAPEGAGHHHGHHH---GCQHHC--DGDGVEPTGAQKAFIRFAKAIRWTELANYLR 120
               CA E + H HGHHH   GCQHHC  D DGVE TGAQKAF+RFA+AIRWT+LANYLR
Sbjct: 61  GHFRCAAEASDHRHGHHHHHHGCQHHCGGDSDGVELTGAQKAFVRFAEAIRWTDLANYLR 120

Query: 121 EHLQLCCGSAALFLTAAACPYLVPKPAVKPLQNVLVAVAFPLVGVSASLDALTDISGGKV 180
           EHL LCCGSAALF+TAAACPYLVPKPAVKPLQNV +AVAFPLVGVSASLDALTDISGG+V
Sbjct: 121 EHLHLCCGSAALFVTAAACPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTDISGGRV 180

Query: 181 NIHVLMALAAFASIFMGNPLEGGLLLVMFNLAHIAEEYFTKQSMIDVKELKESHPDFALV 240
           NIHVLMALAAFASIFMGNPLEGGLLLVMFN+AHIAEEYFTKQSMIDVKELKESHPDFALV
Sbjct: 181 NIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPDFALV 240

Query: 241 LDMNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHLTGEV 300
           LD+NDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHLTGEV
Sbjct: 241 LDVNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHLTGEV 300

Query: 301 KPLEIKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWLDEFG 360
           KPL+IKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWLDEFG
Sbjct: 301 KPLQIKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWLDEFG 360

Query: 361 ENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPLAYAI 420
           ENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPLAYAI
Sbjct: 361 ENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPLAYAI 420

Query: 421 AISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIFGHKVGGEKTHL 480
           AISSCARKGILLKGGHVLDAMAS HTVAFDKTGTLTTGGLVFKAIEPI+GHK+GG+K+H 
Sbjct: 421 AISSCARKGILLKGGHVLDAMASSHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGDKSHF 480

Query: 481 ASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSAGKDLPSISVESFEYFPGRGLIATL 540
           ASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHS GKDLPSISVESFEYFPGRGLIATL
Sbjct: 481 ASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVESFEYFPGRGLIATL 540

Query: 541 HGIKSGIGGKLRKASLGSVDFITSFCKSENESRKIKEAVRASSYGSEFVHAALSVDQKVT 600
           HGIKSGIGGKLRKASLGSVDFITSFCKSENESRKIK+AVRAS+YGSEFVHAALSVDQKVT
Sbjct: 541 HGIKSGIGGKLRKASLGSVDFITSFCKSENESRKIKDAVRASTYGSEFVHAALSVDQKVT 600

Query: 601 LIHLEDQPRPGMLNAISELQDHAKVRVMMLTGDHDSSAWKVANAVGINEVYYSLKPEDKL 660
           LIHLEDQPRPG+++AI+ELQD  K+RVMMLTGDHDSSAWKVANAVGINEVYYSLKPEDKL
Sbjct: 601 LIHLEDQPRPGVVDAIAELQDQGKLRVMMLTGDHDSSAWKVANAVGINEVYYSLKPEDKL 660

Query: 661 AHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLARRASATATAVADVLLLQDNISCV 720
           AHVKEISREMGGGLIMVGEGINDAPALAAATVG+VLARRASATATAVADVLLLQD+IS V
Sbjct: 661 AHVKEISREMGGGLIMVGEGINDAPALAAATVGMVLARRASATATAVADVLLLQDSISAV 720

Query: 721 PFCIAKSRQTTSLVKQNVTLALSSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLNSIRA 780
           PFCIAKSRQTTSLVKQNVTLALSSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLNS+RA
Sbjct: 721 PFCIAKSRQTTSLVKQNVTLALSSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLNSVRA 780

Query: 781 LNQPSWSWKQDFQNLVRDARSRVLATRQTSSGTIQTA 805
           LNQPSWSWKQDFQNL+ + RS++    +TSSGTIQTA
Sbjct: 781 LNQPSWSWKQDFQNLMHNPRSKLNPKPETSSGTIQTA 817

BLAST of MC02g1308 vs. NCBI nr
Match: XP_038901377.1 (probable cadmium/zinc-transporting ATPase HMA1, chloroplastic isoform X1 [Benincasa hispida])

HSP 1 Score: 1387 bits (3591), Expect = 0.0
Identity = 724/831 (87.12%), Postives = 757/831 (91.10%), Query Frame = 0

Query: 1   MDALFVH-------HSYIRRTTNDGAHSTAIPVPRFSLRSSLIGIKLLHLHSKNFPYPRL 60
           MD LFV        H  + R TN GA S   P+ RFS RSSLI +    +  K   YPR 
Sbjct: 1   MDTLFVPISFSESPHPLLTRNTNAGAQSRIGPLSRFSPRSSLIRLNRFPIPCKKISYPRF 60

Query: 61  RRIPCAPEGAGHHHGHHH---GCQHHC--DGDGVEPTGAQKAFIRFAKAIRWTELANYLR 120
               CA E + H HGHHH   GCQHHC  D DGVE TGAQKAF+RFA+AIRWT+LANYLR
Sbjct: 61  GHFRCAAEASDHRHGHHHHHHGCQHHCGGDSDGVELTGAQKAFVRFAEAIRWTDLANYLR 120

Query: 121 EHLQLCCGSAALFLTAAACPYLVPKPAVKPLQNVLVAVAFPLVGVSASLDALTDISGGKV 180
           EHL LCCGSAALF+TAAACPYLVPKPAVKPLQNV +AVAFPLVGVSASLDALTDISGG+V
Sbjct: 121 EHLHLCCGSAALFVTAAACPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTDISGGRV 180

Query: 181 NIHVLMALAAFASIFMGNPLEGGLLLVMFNLAHIAEEYFTKQSMIDVKELKESHPDFALV 240
           NIHVLMALAAFASIFMGNPLEGGLLLVMFN+AHIAEEYFTKQSMIDVKELKESHPDFALV
Sbjct: 181 NIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPDFALV 240

Query: 241 LDMNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHLTGEV 300
           LD+NDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHLTGEV
Sbjct: 241 LDVNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHLTGEV 300

Query: 301 KPLEIKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWLDEFG 360
           KPL+IKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWLDEFG
Sbjct: 301 KPLQIKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWLDEFG 360

Query: 361 ENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPLAYAI 420
           ENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPLAYAI
Sbjct: 361 ENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPLAYAI 420

Query: 421 AISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIFGHKVGGEKTHL 480
           AISSCARKGILLKGGHVLDAMAS HTVAFDKTGTLTTGGLVFKAIEPI+GHK+GG+K+H 
Sbjct: 421 AISSCARKGILLKGGHVLDAMASSHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGDKSHF 480

Query: 481 ASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSAGKDLPSISVESFEYFPGRGLIATL 540
           ASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHS GKDLPSISVESFEYFPGRGLIATL
Sbjct: 481 ASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVESFEYFPGRGLIATL 540

Query: 541 HGIK--------------SGIGGKLRKASLGSVDFITSFCKSENESRKIKEAVRASSYGS 600
           HGIK              SGIGGKLRKASLGSVDFITSFCKSENESRKIK+AVRAS+YGS
Sbjct: 541 HGIKVEGEEYLHSISILQSGIGGKLRKASLGSVDFITSFCKSENESRKIKDAVRASTYGS 600

Query: 601 EFVHAALSVDQKVTLIHLEDQPRPGMLNAISELQDHAKVRVMMLTGDHDSSAWKVANAVG 660
           EFVHAALSVDQKVTLIHLEDQPRPG+++AI+ELQD  K+RVMMLTGDHDSSAWKVANAVG
Sbjct: 601 EFVHAALSVDQKVTLIHLEDQPRPGVVDAIAELQDQGKLRVMMLTGDHDSSAWKVANAVG 660

Query: 661 INEVYYSLKPEDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLARRASATATA 720
           INEVYYSLKPEDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVG+VLARRASATATA
Sbjct: 661 INEVYYSLKPEDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGMVLARRASATATA 720

Query: 721 VADVLLLQDNISCVPFCIAKSRQTTSLVKQNVTLALSSILLASLPSVLGFLPLWLTVLLH 780
           VADVLLLQD+IS VPFCIAKSRQTTSLVKQNVTLALSSILLASLPSVLGFLPLWLTVLLH
Sbjct: 721 VADVLLLQDSISAVPFCIAKSRQTTSLVKQNVTLALSSILLASLPSVLGFLPLWLTVLLH 780

Query: 781 EGGTLLVCLNSIRALNQPSWSWKQDFQNLVRDARSRVLATRQTSSGTIQTA 805
           EGGTLLVCLNS+RALNQPSWSWKQDFQNL+ + RS++    +TSSGTIQTA
Sbjct: 781 EGGTLLVCLNSVRALNQPSWSWKQDFQNLMHNPRSKLNPKPETSSGTIQTA 831

BLAST of MC02g1308 vs. NCBI nr
Match: XP_023533453.1 (probable cadmium/zinc-transporting ATPase HMA1, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1382 bits (3576), Expect = 0.0
Identity = 720/819 (87.91%), Postives = 753/819 (91.94%), Query Frame = 0

Query: 1   MDALFVH-------HSYIRRTTNDGAHSTAIPVPRFSLRSSLIGIKLLHLHSKNFPYPRL 60
           MD LFV        HS + RTTN GA S   PV  FSLRSS+I               RL
Sbjct: 1   MDTLFVPITFSKPIHSLLTRTTNAGARSRIGPVSCFSLRSSVI---------------RL 60

Query: 61  RRIPCAPEGA----GHHHGHHHGCQHHC--DGDGVEPTGAQKAFIRFAKAIRWTELANYL 120
           +R  CA + +    GHHH HHH CQHHC  DGDGVE TGAQKAF+RFAKAIRWT+LANYL
Sbjct: 61  KRFRCAADASDHRHGHHHHHHHSCQHHCGGDGDGVELTGAQKAFVRFAKAIRWTDLANYL 120

Query: 121 REHLQLCCGSAALFLTAAACPYLVPKPAVKPLQNVLVAVAFPLVGVSASLDALTDISGGK 180
           REHL LCCGSAALF+TAAACPYLVPKPAVKPLQNV +AVAFPLVGVSASLDALTDISGGK
Sbjct: 121 REHLHLCCGSAALFITAAACPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTDISGGK 180

Query: 181 VNIHVLMALAAFASIFMGNPLEGGLLLVMFNLAHIAEEYFTKQSMIDVKELKESHPDFAL 240
           VNIHVLMALAAFASIFMGNPLEGGLLLVMFNLAHIAEEYFTKQSM DVKELKESHPDFAL
Sbjct: 181 VNIHVLMALAAFASIFMGNPLEGGLLLVMFNLAHIAEEYFTKQSMTDVKELKESHPDFAL 240

Query: 241 VLDMNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHLTGE 300
           VLDMNDDNLPDTSDLEHR+VPVRD+EVGSYI+VGAGESVPVDCEVY GCATITVEHLTGE
Sbjct: 241 VLDMNDDNLPDTSDLEHRKVPVRDIEVGSYIMVGAGESVPVDCEVYHGCATITVEHLTGE 300

Query: 301 VKPLEIKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWLDEF 360
           +KPL+IKVG+RVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWLDEF
Sbjct: 301 IKPLQIKVGQRVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWLDEF 360

Query: 361 GENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPLAYA 420
           GENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALG MVAASPCALAAAPLAYA
Sbjct: 361 GENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGFMVAASPCALAAAPLAYA 420

Query: 421 IAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIFGHKVGGEKTH 480
           IAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPI+GHK+GG+ +H
Sbjct: 421 IAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGDNSH 480

Query: 481 LASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSAGKDLPSISVESFEYFPGRGLIAT 540
           LASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHS GK+LPSISVESFEYFPGRGLIAT
Sbjct: 481 LASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKNLPSISVESFEYFPGRGLIAT 540

Query: 541 LHGIKSGIGGKLRKASLGSVDFITSFCKSENESRKIKEAVRASSYGSEFVHAALSVDQKV 600
           LHGIKSGIGGKLRKASLGSVDFITSFCKSENESRKIKEAVRASSYGSEFVHAALSVDQKV
Sbjct: 541 LHGIKSGIGGKLRKASLGSVDFITSFCKSENESRKIKEAVRASSYGSEFVHAALSVDQKV 600

Query: 601 TLIHLEDQPRPGMLNAISELQDHAKVRVMMLTGDHDSSAWKVANAVGINEVYYSLKPEDK 660
           TLIHLEDQPR G+L+AI+ELQD A +RVMMLTGDHDSSAWK+ANAVGINEVYYSLKPEDK
Sbjct: 601 TLIHLEDQPRAGVLDAIAELQDQANLRVMMLTGDHDSSAWKIANAVGINEVYYSLKPEDK 660

Query: 661 LAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLARRASATATAVADVLLLQDNISC 720
           LAHVKEISRE GGGLIMVGEGINDAPALAAATVGIVLA RASATATAVADVLLLQD+IS 
Sbjct: 661 LAHVKEISREKGGGLIMVGEGINDAPALAAATVGIVLACRASATATAVADVLLLQDSISG 720

Query: 721 VPFCIAKSRQTTSLVKQNVTLALSSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLNSIR 780
           VPFCIAKSRQTT+LVKQNVTLALSSILLASLPS+LGFLPLWLTVLLHEGGTLLVCLNSIR
Sbjct: 721 VPFCIAKSRQTTALVKQNVTLALSSILLASLPSILGFLPLWLTVLLHEGGTLLVCLNSIR 780

Query: 781 ALNQPSWSWKQDFQNLVRDARSRVLATRQTSSGTIQTAS 806
           ALN PSWSWKQDFQNL+ +ARS +  T + SSGTI+TAS
Sbjct: 781 ALNHPSWSWKQDFQNLIHNARSSLHTTPEPSSGTIRTAS 804

BLAST of MC02g1308 vs. NCBI nr
Match: KAG7035151.1 (putative cadmium/zinc-transporting ATPase HMA1, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1377 bits (3565), Expect = 0.0
Identity = 718/817 (87.88%), Postives = 753/817 (92.17%), Query Frame = 0

Query: 1   MDALFVH-------HSYIRRTTNDGAHSTAIPVPRFSLRSSLIGIKLLHLHSKNFPYPRL 60
           MDALFV        HS + RTTN GA S   PV  FSLRSS+I               RL
Sbjct: 1   MDALFVPITFSKPIHSLLTRTTNAGARSRIGPVSCFSLRSSVI---------------RL 60

Query: 61  RRIPCAPEGAGHHHGHHH--GCQHHC--DGDGVEPTGAQKAFIRFAKAIRWTELANYLRE 120
           +R  CA + + H HGHHH   CQHHC  DGDGVE TGAQKAF+RFA+AIRWT+LANYLRE
Sbjct: 61  KRFRCAADASDHRHGHHHHHSCQHHCGGDGDGVELTGAQKAFVRFAEAIRWTDLANYLRE 120

Query: 121 HLQLCCGSAALFLTAAACPYLVPKPAVKPLQNVLVAVAFPLVGVSASLDALTDISGGKVN 180
           HL LCCGSAALF+TAAACPYLVPKPAVKPLQNV +AVAFPLVGVSASLDALTDISGGKVN
Sbjct: 121 HLHLCCGSAALFITAAACPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTDISGGKVN 180

Query: 181 IHVLMALAAFASIFMGNPLEGGLLLVMFNLAHIAEEYFTKQSMIDVKELKESHPDFALVL 240
           IHVLMALAAFASIFMGNPLEGGLLLVMFNLAHIAEEYFTKQSM DVKELKESHPDFALVL
Sbjct: 181 IHVLMALAAFASIFMGNPLEGGLLLVMFNLAHIAEEYFTKQSMTDVKELKESHPDFALVL 240

Query: 241 DMNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHLTGEVK 300
           DMNDDNLPDTSDLEHR+VPVRD+EVGSYI+VGAGESVPVDCEVY GCATITVEHLTGE+K
Sbjct: 241 DMNDDNLPDTSDLEHRKVPVRDIEVGSYIMVGAGESVPVDCEVYHGCATITVEHLTGEIK 300

Query: 301 PLEIKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWLDEFGE 360
           PL+IKVG+RVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWLDEFGE
Sbjct: 301 PLQIKVGQRVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWLDEFGE 360

Query: 361 NYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPLAYAIA 420
           NYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALG MVAASPCALAAAPLAYAIA
Sbjct: 361 NYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGFMVAASPCALAAAPLAYAIA 420

Query: 421 ISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIFGHKVGGEKTHLA 480
           ISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPI+GHK+GG+ +HLA
Sbjct: 421 ISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGDNSHLA 480

Query: 481 SCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSAGKDLPSISVESFEYFPGRGLIATLH 540
           SCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHS GK+LPSISVESFEYFPGRGLIATLH
Sbjct: 481 SCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKNLPSISVESFEYFPGRGLIATLH 540

Query: 541 GIKSGIGGKLRKASLGSVDFITSFCKSENESRKIKEAVRASSYGSEFVHAALSVDQKVTL 600
           GIKSGIGG LRKASLGSVDFITSFCKSENESRKIKEAVRASSYGSEFVHAALSVDQKVTL
Sbjct: 541 GIKSGIGGPLRKASLGSVDFITSFCKSENESRKIKEAVRASSYGSEFVHAALSVDQKVTL 600

Query: 601 IHLEDQPRPGMLNAISELQDHAKVRVMMLTGDHDSSAWKVANAVGINEVYYSLKPEDKLA 660
           IHLEDQPR G+L+AI+ELQD AK+RVMMLTGDHDSSAWK+ANAVGI+EVYYSLKPEDKLA
Sbjct: 601 IHLEDQPRAGVLDAIAELQDQAKLRVMMLTGDHDSSAWKIANAVGIDEVYYSLKPEDKLA 660

Query: 661 HVKEISREMGGGLIMVGEGINDAPALAAATVGIVLARRASATATAVADVLLLQDNISCVP 720
           HVKEISRE GGGLIMVGEGINDAPALAAATVGIVLA RASATATAVADVLLLQD+IS VP
Sbjct: 661 HVKEISREKGGGLIMVGEGINDAPALAAATVGIVLACRASATATAVADVLLLQDSISGVP 720

Query: 721 FCIAKSRQTTSLVKQNVTLALSSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLNSIRAL 780
           FCIAKSRQTT+LVKQNVTLALSSILLASLPS+LGFLPLWLTVLLHEGGTLLVCLNSIRAL
Sbjct: 721 FCIAKSRQTTALVKQNVTLALSSILLASLPSILGFLPLWLTVLLHEGGTLLVCLNSIRAL 780

Query: 781 NQPSWSWKQDFQNLVRDARSRVLATRQTSSGTIQTAS 806
           N PSWSWKQDFQNL+ +ARS +  T + SSGTI+TAS
Sbjct: 781 NHPSWSWKQDFQNLIHNARSSLHTTPEPSSGTIRTAS 802

BLAST of MC02g1308 vs. ExPASy TrEMBL
Match: A0A6J1DBA2 (probable cadmium/zinc-transporting ATPase HMA1, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111018894 PE=3 SV=1)

HSP 1 Score: 1575 bits (4078), Expect = 0.0
Identity = 807/807 (100.00%), Postives = 807/807 (100.00%), Query Frame = 0

Query: 1   MDALFVHHSYIRRTTNDGAHSTAIPVPRFSLRSSLIGIKLLHLHSKNFPYPRLRRIPCAP 60
           MDALFVHHSYIRRTTNDGAHSTAIPVPRFSLRSSLIGIKLLHLHSKNFPYPRLRRIPCAP
Sbjct: 1   MDALFVHHSYIRRTTNDGAHSTAIPVPRFSLRSSLIGIKLLHLHSKNFPYPRLRRIPCAP 60

Query: 61  EGAGHHHGHHHGCQHHCDGDGVEPTGAQKAFIRFAKAIRWTELANYLREHLQLCCGSAAL 120
           EGAGHHHGHHHGCQHHCDGDGVEPTGAQKAFIRFAKAIRWTELANYLREHLQLCCGSAAL
Sbjct: 61  EGAGHHHGHHHGCQHHCDGDGVEPTGAQKAFIRFAKAIRWTELANYLREHLQLCCGSAAL 120

Query: 121 FLTAAACPYLVPKPAVKPLQNVLVAVAFPLVGVSASLDALTDISGGKVNIHVLMALAAFA 180
           FLTAAACPYLVPKPAVKPLQNVLVAVAFPLVGVSASLDALTDISGGKVNIHVLMALAAFA
Sbjct: 121 FLTAAACPYLVPKPAVKPLQNVLVAVAFPLVGVSASLDALTDISGGKVNIHVLMALAAFA 180

Query: 181 SIFMGNPLEGGLLLVMFNLAHIAEEYFTKQSMIDVKELKESHPDFALVLDMNDDNLPDTS 240
           SIFMGNPLEGGLLLVMFNLAHIAEEYFTKQSMIDVKELKESHPDFALVLDMNDDNLPDTS
Sbjct: 181 SIFMGNPLEGGLLLVMFNLAHIAEEYFTKQSMIDVKELKESHPDFALVLDMNDDNLPDTS 240

Query: 241 DLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHLTGEVKPLEIKVGERVP 300
           DLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHLTGEVKPLEIKVGERVP
Sbjct: 241 DLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHLTGEVKPLEIKVGERVP 300

Query: 301 GGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWLDEFGENYSKVVVVLSV 360
           GGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWLDEFGENYSKVVVVLSV
Sbjct: 301 GGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWLDEFGENYSKVVVVLSV 360

Query: 361 AVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPLAYAIAISSCARKGILL 420
           AVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPLAYAIAISSCARKGILL
Sbjct: 361 AVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPLAYAIAISSCARKGILL 420

Query: 421 KGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIFGHKVGGEKTHLASCCIPSCEKEA 480
           KGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIFGHKVGGEKTHLASCCIPSCEKEA
Sbjct: 421 KGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIFGHKVGGEKTHLASCCIPSCEKEA 480

Query: 481 LAVAAAMEKGTTHPIGRAVVDHSAGKDLPSISVESFEYFPGRGLIATLHGIKSGIGGKLR 540
           LAVAAAMEKGTTHPIGRAVVDHSAGKDLPSISVESFEYFPGRGLIATLHGIKSGIGGKLR
Sbjct: 481 LAVAAAMEKGTTHPIGRAVVDHSAGKDLPSISVESFEYFPGRGLIATLHGIKSGIGGKLR 540

Query: 541 KASLGSVDFITSFCKSENESRKIKEAVRASSYGSEFVHAALSVDQKVTLIHLEDQPRPGM 600
           KASLGSVDFITSFCKSENESRKIKEAVRASSYGSEFVHAALSVDQKVTLIHLEDQPRPGM
Sbjct: 541 KASLGSVDFITSFCKSENESRKIKEAVRASSYGSEFVHAALSVDQKVTLIHLEDQPRPGM 600

Query: 601 LNAISELQDHAKVRVMMLTGDHDSSAWKVANAVGINEVYYSLKPEDKLAHVKEISREMGG 660
           LNAISELQDHAKVRVMMLTGDHDSSAWKVANAVGINEVYYSLKPEDKLAHVKEISREMGG
Sbjct: 601 LNAISELQDHAKVRVMMLTGDHDSSAWKVANAVGINEVYYSLKPEDKLAHVKEISREMGG 660

Query: 661 GLIMVGEGINDAPALAAATVGIVLARRASATATAVADVLLLQDNISCVPFCIAKSRQTTS 720
           GLIMVGEGINDAPALAAATVGIVLARRASATATAVADVLLLQDNISCVPFCIAKSRQTTS
Sbjct: 661 GLIMVGEGINDAPALAAATVGIVLARRASATATAVADVLLLQDNISCVPFCIAKSRQTTS 720

Query: 721 LVKQNVTLALSSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLNSIRALNQPSWSWKQDF 780
           LVKQNVTLALSSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLNSIRALNQPSWSWKQDF
Sbjct: 721 LVKQNVTLALSSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLNSIRALNQPSWSWKQDF 780

Query: 781 QNLVRDARSRVLATRQTSSGTIQTASL 807
           QNLVRDARSRVLATRQTSSGTIQTASL
Sbjct: 781 QNLVRDARSRVLATRQTSSGTIQTASL 807

BLAST of MC02g1308 vs. ExPASy TrEMBL
Match: A0A1S3CI84 (probable cadmium/zinc-transporting ATPase HMA1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103501066 PE=3 SV=1)

HSP 1 Score: 1372 bits (3551), Expect = 0.0
Identity = 715/821 (87.09%), Postives = 749/821 (91.23%), Query Frame = 0

Query: 1   MDALFVH-------HSYIRRTTNDGAHSTAIPVPRFSLRSSLIGIKLLHLHSKNFPYPRL 60
           MD LFV        H  +   TN G+     PV RFS  SSLI +    +  K   YPR 
Sbjct: 1   MDTLFVPITFSELPHPLLTSNTNAGSQPRIGPVSRFSPLSSLIHLNRFPICFKKLSYPRF 60

Query: 61  RRIPCAPEGA----GHHHGHHH---GCQHHCDGDG--VEPTGAQKAFIRFAKAIRWTELA 120
               CA E +    GHHHGHHH   GCQHHC GD   VE TG QKAF+RFA+AIRWT+LA
Sbjct: 61  GHFRCAAEASDHRRGHHHGHHHHHHGCQHHCSGDSDRVELTGTQKAFVRFAEAIRWTDLA 120

Query: 121 NYLREHLQLCCGSAALFLTAAACPYLVPKPAVKPLQNVLVAVAFPLVGVSASLDALTDIS 180
           NYLREHL +CCGSAALF+TAAA PYLVPKPAVKPLQNV +AVAFPLVGVSASLDALTDIS
Sbjct: 121 NYLREHLHMCCGSAALFVTAAAFPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTDIS 180

Query: 181 GGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNLAHIAEEYFTKQSMIDVKELKESHPD 240
           GGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFN+AHIAEEYFTKQSMIDVKELKESHPD
Sbjct: 181 GGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPD 240

Query: 241 FALVLDMNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHL 300
           FALVLD+NDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHL
Sbjct: 241 FALVLDVNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHL 300

Query: 301 TGEVKPLEIKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWL 360
           TGEVKPL+IKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWL
Sbjct: 301 TGEVKPLQIKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWL 360

Query: 361 DEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPL 420
           DEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPL
Sbjct: 361 DEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPL 420

Query: 421 AYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIFGHKVGGE 480
           AYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPI+GHK+GG+
Sbjct: 421 AYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGD 480

Query: 481 KTHLASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSAGKDLPSISVESFEYFPGRGL 540
           K+  ASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHS GKDLPSISVES EYFPGRGL
Sbjct: 481 KSQFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVESTEYFPGRGL 540

Query: 541 IATLHGIKSGIGGKLRKASLGSVDFITSFCKSENESRKIKEAVRASSYGSEFVHAALSVD 600
           IATLHGIKSGIGGKLRKAS GSVDFITSFCKSENESR IK+AVRAS+YGSEFVHAALSVD
Sbjct: 541 IATLHGIKSGIGGKLRKASFGSVDFITSFCKSENESRMIKDAVRASTYGSEFVHAALSVD 600

Query: 601 QKVTLIHLEDQPRPGMLNAISELQDHAKVRVMMLTGDHDSSAWKVANAVGINEVYYSLKP 660
           QKVTLIHLEDQPRPG+++AI+ELQ   K+RVMMLTGDHDSSAWKVANAVGINEVYYSLKP
Sbjct: 601 QKVTLIHLEDQPRPGVIDAIAELQHLGKLRVMMLTGDHDSSAWKVANAVGINEVYYSLKP 660

Query: 661 EDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLARRASATATAVADVLLLQDN 720
           EDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLA RASATATAVADVLLLQD+
Sbjct: 661 EDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLLLQDS 720

Query: 721 ISCVPFCIAKSRQTTSLVKQNVTLALSSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLN 780
           IS VPFCIAKSRQTTSL+KQNVTLAL+SILLASLPSVLGFLPLWLTVLLHEGGTLLVCLN
Sbjct: 721 ISGVPFCIAKSRQTTSLIKQNVTLALTSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLN 780

Query: 781 SIRALNQPSWSWKQDFQNLVRDARSRVLATRQTSSGTIQTA 805
           S+RALN PSWSWKQDFQNL+ +ARS++  T +TSSGTIQTA
Sbjct: 781 SVRALNHPSWSWKQDFQNLIHNARSKLNTTPETSSGTIQTA 821

BLAST of MC02g1308 vs. ExPASy TrEMBL
Match: A0A6J1L1A5 (probable cadmium/zinc-transporting ATPase HMA1, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111499516 PE=3 SV=1)

HSP 1 Score: 1371 bits (3549), Expect = 0.0
Identity = 715/820 (87.20%), Postives = 751/820 (91.59%), Query Frame = 0

Query: 1   MDALFVH-------HSYIRRTTNDGAHSTAIPVPRFSLRSSLIGIKLLHLHSKNFPYPRL 60
           MD LF+        HS + RTTN GA S   PV  FSLRSS I               RL
Sbjct: 1   MDTLFIPITFSKPIHSLLTRTTNAGARSRIGPVSCFSLRSSAI---------------RL 60

Query: 61  RRIPCAPEGAGHHHGHHH-----GCQHHC--DGDGVEPTGAQKAFIRFAKAIRWTELANY 120
           +R  CA + + H HGHHH      CQHHC  DGDGVE TGAQKAF+ FA+AIRWT+LANY
Sbjct: 61  KRFRCAADASDHRHGHHHHHHHHSCQHHCGGDGDGVELTGAQKAFVGFAEAIRWTDLANY 120

Query: 121 LREHLQLCCGSAALFLTAAACPYLVPKPAVKPLQNVLVAVAFPLVGVSASLDALTDISGG 180
           LREHL LCCGSAALF+TAAACPYLVPKPAVKPLQNV +AVAFPLVGVSASLDALTDISGG
Sbjct: 121 LREHLHLCCGSAALFITAAACPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTDISGG 180

Query: 181 KVNIHVLMALAAFASIFMGNPLEGGLLLVMFNLAHIAEEYFTKQSMIDVKELKESHPDFA 240
           KVNIHVLMALAAFASIFMGNPLEGGLLLVMFNLAHIAEEYFTKQSM DVKELKESHPDFA
Sbjct: 181 KVNIHVLMALAAFASIFMGNPLEGGLLLVMFNLAHIAEEYFTKQSMTDVKELKESHPDFA 240

Query: 241 LVLDMNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHLTG 300
           LVLDMNDDNLPDTSDLEHR+VPVRD+EVGSYI+VGAGESVPVDCEVY GCATITVEHLTG
Sbjct: 241 LVLDMNDDNLPDTSDLEHRKVPVRDIEVGSYIMVGAGESVPVDCEVYHGCATITVEHLTG 300

Query: 301 EVKPLEIKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWLDE 360
           E+KPL+IKVG+RVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQ NKPKLQRWLDE
Sbjct: 301 EIKPLQIKVGQRVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQQNKPKLQRWLDE 360

Query: 361 FGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPLAY 420
           FGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALG MVAASPCALAAAPLAY
Sbjct: 361 FGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGFMVAASPCALAAAPLAY 420

Query: 421 AIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIFGHKVGGEKT 480
           AIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPI+GHK+GG+K+
Sbjct: 421 AIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGDKS 480

Query: 481 HLASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSAGKDLPSISVESFEYFPGRGLIA 540
           HLASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHS GK+LPSISVESFEYFPGRGLIA
Sbjct: 481 HLASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKNLPSISVESFEYFPGRGLIA 540

Query: 541 TLHGIKSGIGGKLRKASLGSVDFITSFCKSENESRKIKEAVRASSYGSEFVHAALSVDQK 600
           TLHGIKSGIGGKLRKASLGSVDFITSFCKSENESRKIKEAVRASS+GSEFVHAALSVDQK
Sbjct: 541 TLHGIKSGIGGKLRKASLGSVDFITSFCKSENESRKIKEAVRASSFGSEFVHAALSVDQK 600

Query: 601 VTLIHLEDQPRPGMLNAISELQDHAKVRVMMLTGDHDSSAWKVANAVGINEVYYSLKPED 660
           VTLIHLEDQPR G+L+AI+ELQD AK+RVMMLTGDHDSSAWK+ANAVGI+EVYYSLKPED
Sbjct: 601 VTLIHLEDQPRAGVLDAIAELQDQAKLRVMMLTGDHDSSAWKIANAVGISEVYYSLKPED 660

Query: 661 KLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLARRASATATAVADVLLLQDNIS 720
           KLAHVKEISRE GGGLIMVGEGINDAPALAAATVGIVLA RASATATAVADVLLLQD+IS
Sbjct: 661 KLAHVKEISREKGGGLIMVGEGINDAPALAAATVGIVLACRASATATAVADVLLLQDSIS 720

Query: 721 CVPFCIAKSRQTTSLVKQNVTLALSSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLNSI 780
            VPFCIAKSRQTT+LVKQNVTLALSSILLASLPS+LGFLPLWLTVLLHEGGTLLVCLNSI
Sbjct: 721 GVPFCIAKSRQTTALVKQNVTLALSSILLASLPSILGFLPLWLTVLLHEGGTLLVCLNSI 780

Query: 781 RALNQPSWSWKQDFQNLVRDARSRVLATRQTSSGTIQTAS 806
           RALN PSWSWKQDFQNL+ +ARS +  T + SSGTI+TAS
Sbjct: 781 RALNHPSWSWKQDFQNLIHNARSSLHTTPEPSSGTIRTAS 805

BLAST of MC02g1308 vs. ExPASy TrEMBL
Match: A0A6J1G6Z6 (probable cadmium/zinc-transporting ATPase HMA1, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111451327 PE=3 SV=1)

HSP 1 Score: 1368 bits (3541), Expect = 0.0
Identity = 715/818 (87.41%), Postives = 750/818 (91.69%), Query Frame = 0

Query: 1   MDALFVH-------HSYIRRTTNDGAHSTAIPVPRFSLRSSLIGIKLLHLHSKNFPYPRL 60
           MD LFV        HS + RTTN GA S   PV  FSLRSS+I               RL
Sbjct: 1   MDTLFVPITFSKPIHSLLTRTTNAGARSRIGPVSCFSLRSSVI---------------RL 60

Query: 61  RRIPCAPEGAGHHHGHHH---GCQHHC--DGDGVEPTGAQKAFIRFAKAIRWTELANYLR 120
           +R  CA + + H HGHHH    CQHHC  DGDGVE TGAQKAF+RFA+AIRWT+LANYLR
Sbjct: 61  KRFRCAADASDHRHGHHHHHHSCQHHCGGDGDGVELTGAQKAFVRFAEAIRWTDLANYLR 120

Query: 121 EHLQLCCGSAALFLTAAACPYLVPKPAVKPLQNVLVAVAFPLVGVSASLDALTDISGGKV 180
           EHL LCCGSAALF+TAAACPYLVPKPAVK LQNV +AVAFPLVGVSASLDALTDISGGKV
Sbjct: 121 EHLHLCCGSAALFITAAACPYLVPKPAVKLLQNVFIAVAFPLVGVSASLDALTDISGGKV 180

Query: 181 NIHVLMALAAFASIFMGNPLEGGLLLVMFNLAHIAEEYFTKQSMIDVKELKESHPDFALV 240
           NIHVLMALAAFASIFMGNPLEGGLLLVMFNLAHIAEEYFTKQSM DVKELKESHPDFALV
Sbjct: 181 NIHVLMALAAFASIFMGNPLEGGLLLVMFNLAHIAEEYFTKQSMTDVKELKESHPDFALV 240

Query: 241 LDMNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHLTGEV 300
           LDMNDDNLPDTSDLEHR+VPVRD+EVGSYI+VGAGESVPVDCEVY GCATITVEHLTGE+
Sbjct: 241 LDMNDDNLPDTSDLEHRKVPVRDIEVGSYIMVGAGESVPVDCEVYHGCATITVEHLTGEI 300

Query: 301 KPLEIKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWLDEFG 360
           KPL+IKVG+RVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWLDEFG
Sbjct: 301 KPLQIKVGQRVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWLDEFG 360

Query: 361 ENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPLAYAI 420
           ENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALG MVAASPCALAAAPLAYAI
Sbjct: 361 ENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGFMVAASPCALAAAPLAYAI 420

Query: 421 AISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIFGHKVGGEKTHL 480
           AISSCARKGILLKGGHVLDAMASC TVAFDKTGTLTTGGLVFKAIEPI+GHK+GG+ +HL
Sbjct: 421 AISSCARKGILLKGGHVLDAMASCDTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGDNSHL 480

Query: 481 ASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSAGKDLPSISVESFEYFPGRGLIATL 540
           ASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHS GK+LPSISVESFEYFPGRGLIATL
Sbjct: 481 ASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKNLPSISVESFEYFPGRGLIATL 540

Query: 541 HGIKSGIGGKLRKASLGSVDFITSFCKSENESRKIKEAVRASSYGSEFVHAALSVDQKVT 600
           HGIKSGIGG LRKASLGSVDFITSFCKSENESRKIKEAVRASSYGSEFVHAALSVDQKVT
Sbjct: 541 HGIKSGIGGPLRKASLGSVDFITSFCKSENESRKIKEAVRASSYGSEFVHAALSVDQKVT 600

Query: 601 LIHLEDQPRPGMLNAISELQDHAKVRVMMLTGDHDSSAWKVANAVGINEVYYSLKPEDKL 660
           LIHLEDQPR G+L+AI+ELQD AK+RVMMLTGDHDSSAWK+ANAVGI+EVYYSLKPEDKL
Sbjct: 601 LIHLEDQPRAGVLDAIAELQDQAKLRVMMLTGDHDSSAWKIANAVGIDEVYYSLKPEDKL 660

Query: 661 AHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLARRASATATAVADVLLLQDNISCV 720
           AHVKEISRE GGGLIMVGEGINDAPALAAATVGIVLA RASATATAVADVLLLQD+IS V
Sbjct: 661 AHVKEISREKGGGLIMVGEGINDAPALAAATVGIVLACRASATATAVADVLLLQDSISGV 720

Query: 721 PFCIAKSRQTTSLVKQNVTLALSSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLNSIRA 780
           PFCIAKSRQTT+LVKQNVTLALSSILLASLPS+LGFLPLWLTVLLHEGGTLLVCLNSIRA
Sbjct: 721 PFCIAKSRQTTALVKQNVTLALSSILLASLPSILGFLPLWLTVLLHEGGTLLVCLNSIRA 780

Query: 781 LNQPSWSWKQDFQNLVRDARSRVLATRQTSSGTIQTAS 806
           LN PSWSWKQDFQNL+ +ARS +  T + SSGTI+TAS
Sbjct: 781 LNHPSWSWKQDFQNLIHNARSSLHTTPEPSSGTIRTAS 803

BLAST of MC02g1308 vs. ExPASy TrEMBL
Match: A0A0A0LN89 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G433360 PE=3 SV=1)

HSP 1 Score: 1352 bits (3500), Expect = 0.0
Identity = 708/821 (86.24%), Postives = 742/821 (90.38%), Query Frame = 0

Query: 1   MDALFVH-------HSYIRRTTNDGAHSTAIPVPRFSLRSSLIGIKLLHLHSKNFPYPRL 60
           MD LFV        H  +   TN  + S   PV RFS  SSLI +       K   YPR 
Sbjct: 1   MDTLFVPITFSELPHPLLTCNTNAASQSRVGPVSRFSPLSSLIHLNRFPTRFKKLSYPRF 60

Query: 61  RRIPCAPEGAGHHHGHHHG-------CQHHCDGD--GVEPTGAQKAFIRFAKAIRWTELA 120
               CA E +GH HGHHHG       CQHHC GD  GVE TGAQKAF+RFA+AIRWT+LA
Sbjct: 61  GHFRCAAEASGHRHGHHHGHHHHHHGCQHHCSGDSDGVELTGAQKAFVRFAEAIRWTDLA 120

Query: 121 NYLREHLQLCCGSAALFLTAAACPYLVPKPAVKPLQNVLVAVAFPLVGVSASLDALTDIS 180
           NYLREHL +CCGSAALF+TAAA PYLVPKPAVKPLQNV +AVAFPLVGVSASLDALTDIS
Sbjct: 121 NYLREHLHMCCGSAALFVTAAAFPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTDIS 180

Query: 181 GGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNLAHIAEEYFTKQSMIDVKELKESHPD 240
           GGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFN+AHIAEEYFTKQSMIDVKELKESHPD
Sbjct: 181 GGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPD 240

Query: 241 FALVLDMNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHL 300
           FALVLD+NDDNLPDTSDLEHRRVPVRDV+VGSYILVGAGESVPVDCEVYQGCATITVEHL
Sbjct: 241 FALVLDVNDDNLPDTSDLEHRRVPVRDVKVGSYILVGAGESVPVDCEVYQGCATITVEHL 300

Query: 301 TGEVKPLEIKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWL 360
           TGEV+PL+IKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWL
Sbjct: 301 TGEVRPLQIKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWL 360

Query: 361 DEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPL 420
           DEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPL
Sbjct: 361 DEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPL 420

Query: 421 AYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIFGHKVGGE 480
           AYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPI+GHK+GG+
Sbjct: 421 AYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGD 480

Query: 481 KTHLASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSAGKDLPSISVESFEYFPGRGL 540
           K+  ASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHS GKDLPSISVES EYFPGRGL
Sbjct: 481 KSQFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVESTEYFPGRGL 540

Query: 541 IATLHGIKSGIGGKLRKASLGSVDFITSFCKSENESRKIKEAVRASSYGSEFVHAALSVD 600
           IATLHGIKSGIGGKLRKASLGSVDFITSFCKSENESR IK+AVRAS+YGSEFVHAALSVD
Sbjct: 541 IATLHGIKSGIGGKLRKASLGSVDFITSFCKSENESRMIKDAVRASTYGSEFVHAALSVD 600

Query: 601 QKVTLIHLEDQPRPGMLNAISELQDHAKVRVMMLTGDHDSSAWKVANAVGINEVYYSLKP 660
           QKVTLIHLEDQPRPG+++AI+ELQ   K+RVMMLTGDHDSSAWKVANAVGINEVYYSLKP
Sbjct: 601 QKVTLIHLEDQPRPGVIDAIAELQHLGKLRVMMLTGDHDSSAWKVANAVGINEVYYSLKP 660

Query: 661 EDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLARRASATATAVADVLLLQDN 720
           EDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLA RASATATAVADVLLLQD+
Sbjct: 661 EDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLLLQDS 720

Query: 721 ISCVPFCIAKSRQTTSLVKQNVTLALSSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLN 780
           IS VPFCIAKSRQTTSL+KQNVTLAL+SILLASLPSVLG     L VLLHEGGTLLVCLN
Sbjct: 721 ISGVPFCIAKSRQTTSLIKQNVTLALTSILLASLPSVLG-----LKVLLHEGGTLLVCLN 780

Query: 781 SIRALNQPSWSWKQDFQNLVRDARSRVLATRQTSSGTIQTA 805
           S+RALN PSWSWKQD QNL+ DARS++  T + SSGTIQTA
Sbjct: 781 SVRALNHPSWSWKQDLQNLIHDARSKLNTTPENSSGTIQTA 816

BLAST of MC02g1308 vs. TAIR 10
Match: AT4G37270.1 (heavy metal atpase 1 )

HSP 1 Score: 1084.3 bits (2803), Expect = 0.0e+00
Identity = 553/736 (75.14%), Postives = 630/736 (85.60%), Query Frame = 0

Query: 65  HHHGHHHGCQHHCDGDGVEPTGAQKAFIRFAKAIRWTELANYLREHLQLCCGSAALFLTA 124
           HHH H HGC   C  +    +  QK    FAKAI W  LANYLREHL LCC +AA+FL A
Sbjct: 83  HHHHHQHGC---CSVELKAESKPQKMLFGFAKAIGWVRLANYLREHLHLCCSAAAMFLAA 142

Query: 125 AACPYLVPKPAVKPLQNVLVAVAFPLVGVSASLDALTDISGGKVNIHVLMALAAFASIFM 184
           A CPYL P+P +K LQN  + V FPLVGVSASLDAL DI+GGKVNIHVLMALAAFAS+FM
Sbjct: 143 AVCPYLAPEPYIKSLQNAFMIVGFPLVGVSASLDALMDIAGGKVNIHVLMALAAFASVFM 202

Query: 185 GNPLEGGLLLVMFNLAHIAEEYFTKQSMIDVKELKESHPDFALVLDMNDDNLPDTSDLEH 244
           GN LEGGLLL MFNLAHIAEE+FT +SM+DVKELKES+PD AL++++++ N+P+ SDL +
Sbjct: 203 GNALEGGLLLAMFNLAHIAEEFFTSRSMVDVKELKESNPDSALLIEVHNGNVPNISDLSY 262

Query: 245 RRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHLTGEVKPLEIKVGERVPGGAR 304
           + VPV  VEVGSY+LVG GE VPVDCEVYQG ATIT+EHLTGEVKPLE K G+RVPGGAR
Sbjct: 263 KSVPVHSVEVGSYVLVGTGEIVPVDCEVYQGSATITIEHLTGEVKPLEAKAGDRVPGGAR 322

Query: 305 NLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWLDEFGENYSKVVVVLSVAVAL 364
           NLDGR+IVKATK W +STL++IVQLTEEA  NKPKLQRWLDEFGENYSKVVVVLS+A+A 
Sbjct: 323 NLDGRMIVKATKAWNDSTLNKIVQLTEEAHSNKPKLQRWLDEFGENYSKVVVVLSLAIAF 382

Query: 365 IGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPLAYAIAISSCARKGILLKGGH 424
           +GP LFKWPF+ T   RGSVYRALGLMVAASPCALA APLAYA AISSCARKGILLKG  
Sbjct: 383 LGPFLFKWPFLSTAACRGSVYRALGLMVAASPCALAVAPLAYATAISSCARKGILLKGAQ 442

Query: 425 VLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIFGHKVGGEKTHLASCCIPSCEKEALAVA 484
           VLDA+ASCHT+AFDKTGTLTTGGL  KAIEPI+GH+ GG  + + +CCIP+CEKEALAVA
Sbjct: 443 VLDALASCHTIAFDKTGTLTTGGLTCKAIEPIYGHQ-GGTNSSVITCCIPNCEKEALAVA 502

Query: 485 AAMEKGTTHPIGRAVVDHSAGKDLPSISVESFEYFPGRGLIATLHGIKS-GIGGKLRKAS 544
           AAMEKGTTHPIGRAVVDHS GKDLPSI VESFEYFPGRGL AT++G+K+     +LRKAS
Sbjct: 503 AAMEKGTTHPIGRAVVDHSVGKDLPSIFVESFEYFPGRGLTATVNGVKTVAEESRLRKAS 562

Query: 545 LGSVDFITSFCKSENESRKIKEAVRASSYGSEFVHAALSVDQKVTLIHLEDQPRPGMLNA 604
           LGS++FITS  KSE+ES++IK+AV ASSYG +FVHAALSVDQKVTLIHLEDQPRPG+   
Sbjct: 563 LGSIEFITSLFKSEDESKQIKDAVNASSYGKDFVHAALSVDQKVTLIHLEDQPRPGVSGV 622

Query: 605 ISELQDHAKVRVMMLTGDHDSSAWKVANAVGINEVYYSLKPEDKLAHVKEISREMGGGLI 664
           I+EL+  A++RVMMLTGDHDSSAW+VANAVGI EVY +LKPEDKL HVK I+RE GGGLI
Sbjct: 623 IAELKSWARLRVMMLTGDHDSSAWRVANAVGITEVYCNLKPEDKLNHVKNIAREAGGGLI 682

Query: 665 MVGEGINDAPALAAATVGIVLARRASATATAVADVLLLQDNISCVPFCIAKSRQTTSLVK 724
           MVGEGINDAPALAAATVGIVLA+RASATA AVAD+LLL+DNI+ VPFC+AKSRQTTSLVK
Sbjct: 683 MVGEGINDAPALAAATVGIVLAQRASATAIAVADILLLRDNITGVPFCVAKSRQTTSLVK 742

Query: 725 QNVTLALSSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLNSIRALNQPSWSWKQDFQNL 784
           QNV LAL+SI LA+LPSVLGF+PLWLTVLLHEGGTLLVCLNS+R LN PSWSWKQD  +L
Sbjct: 743 QNVALALTSIFLAALPSVLGFVPLWLTVLLHEGGTLLVCLNSVRGLNDPSWSWKQDIVHL 802

Query: 785 VRDARSRVLATRQTSS 800
           +   RS+   +  ++S
Sbjct: 803 INKLRSQEPTSSSSNS 814

BLAST of MC02g1308 vs. TAIR 10
Match: AT2G19110.1 (heavy metal atpase 4 )

HSP 1 Score: 203.4 bits (516), Expect = 7.0e-52
Identity = 173/636 (27.20%), Postives = 308/636 (48.43%), Query Frame = 0

Query: 144 VAVAFPLVGVSASL-DALTDISGGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNLAHI 203
           +AVA    G+   L  A   I   +++I++L+ +   A++ M + +E   ++ +F ++  
Sbjct: 123 LAVAAVAAGIYPILAKAFASIKRPRIDINILVIITVIATLAMQDFMEAAAVVFLFTISDW 182

Query: 204 AEEYFTKQSMIDVKELKESHPDFALVLDMNDDNLPDTSDLEHRRVPVRDVEVGSYILVGA 263
            E   + ++   ++ L    P  A++ +  ++            V V +V+V + + V A
Sbjct: 183 LETRASYKATSVMQSLMSLAPQKAIIAETGEE------------VEVDEVKVDTVVAVKA 242

Query: 264 GESVPVDCEVYQGCATITVEHLTGEVKPLEIKVGERVPGGARNLDGRIIVKATKTWEEST 323
           GE++P+D  V  G   +  + LTGE  P+  +    V  G  NL+G I VK T    +  
Sbjct: 243 GETIPIDGIVVDGNCEVDEKTLTGEAFPVPKQRDSTVWAGTINLNGYICVKTTSLAGDCV 302

Query: 324 LSRIVQLTEEAQLNKPKLQRWLDEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRG 383
           ++++ +L EEAQ +K K QR +D+  + Y+  ++++S  VA++ P++ K         + 
Sbjct: 303 VAKMAKLVEEAQSSKTKSQRLIDKCSQYYTPAIILVSACVAIV-PVIMK-----VHNLKH 362

Query: 384 SVYRALGLMVAASPCAL-AAAPLAYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTG 443
             + AL ++V+  PC L  + P+A   A++  A  G+L+K    LD ++    VAFDKTG
Sbjct: 363 WFHLALVVLVSGCPCGLILSTPVATFCALTKAATSGLLIKSADYLDTLSKIKIVAFDKTG 422

Query: 444 TLTTGGLVFKAIEPIFGHKVGGEKTHLASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVD 503
           T+T G  +      +   K      +L S          L   +++E  ++HP+   +VD
Sbjct: 423 TITRGEFI------VIDFKSLSRDINLRS---------LLYWVSSVESKSSHPMAATIVD 482

Query: 504 H--SAGKDLPSISVESFEYFPGRGLIATLHGIKSGIGGKLRKASLGSVDFITSFCKSENE 563
           +  S   +     VE ++ FPG G+   + G    IG K   +  G        C +  E
Sbjct: 483 YAKSVSVEPRPEEVEDYQNFPGEGIYGKIDGNDIFIGNKKIASRAG--------CSTVPE 542

Query: 564 SRKIKEAVRASSYGSEFVHAALSVDQKVTLIHLEDQPRPGMLNAISELQDHAKVRVMMLT 623
                  +   + G + V      ++     +L D  R G+  A++EL+    ++  MLT
Sbjct: 543 -------IEVDTKGGKTVGYVYVGERLAGFFNLSDACRSGVSQAMAELKS-LGIKTAMLT 602

Query: 624 GDHDSSAWKVANAVG--INEVYYSLKPEDKLAHVKEISREMGGGLIMVGEGINDAPALAA 683
           GD+ ++A      +G  ++ V+  L PEDK   ++E  +E  G   MVG+G+NDAPALA 
Sbjct: 603 GDNQAAAMHAQEQLGNVLDVVHGDLLPEDKSRIIQEFKKE--GPTAMVGDGVNDAPALAT 662

Query: 684 ATVGIVLARRASATATAVADVLLLQDNISCVPFCIAKSRQTTSLVKQNVTLALSSILLAS 743
           A +GI +    SA AT   +++L+ ++I  +P  +  +R+    V +NV   LS IL A 
Sbjct: 663 ADIGISMGISGSALATQTGNIILMSNDIRRIPQAVKLARRARRKVVENV--CLSIILKAG 705

Query: 744 LPSV--LGFLPLWLTVLLHEGGTLLVCLNSIRALNQ 772
           + ++   G   +W  VL+  G  LLV  NS+  L +
Sbjct: 723 ILALAFAGHPLIWAAVLVDVGTCLLVIFNSMLLLRE 705

BLAST of MC02g1308 vs. TAIR 10
Match: AT4G30110.1 (heavy metal atpase 2 )

HSP 1 Score: 203.0 bits (515), Expect = 9.2e-52
Identity = 171/633 (27.01%), Postives = 308/633 (48.66%), Query Frame = 0

Query: 144 VAVAFPLVGVSASL-DALTDISGGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNLAHI 203
           +AVA  + G+   L  A+  ++  +++I++L+ +   A+I M +  E  +++ +F +A  
Sbjct: 113 LAVAAVVAGIYPILAKAVASLARFRIDINILVVVTVGATIGMQDYTEAAVVVFLFTIAEW 172

Query: 204 AEEYFTKQSMIDVKELKESHPDFALVLDMNDDNLPDTSDLEHRRVPVRDVEVGSYILVGA 263
            +   + ++   ++ L    P  A++ +  ++            V V +++  + I V A
Sbjct: 173 LQSRASYKASAVMQSLMSLAPQKAVIAETGEE------------VEVDELKTNTVIAVKA 232

Query: 264 GESVPVDCEVYQGCATITVEHLTGEVKPLEIKVGERVPGGARNLDGRIIVKATKTWEEST 323
           GE++P+D  V  G   +  + LTGE  P+       V  G  NL+G I V  T   E+  
Sbjct: 233 GETIPIDGVVVDGNCEVDEKTLTGEAFPVPKLKDSTVWAGTINLNGYITVNTTALAEDCV 292

Query: 324 LSRIVQLTEEAQLNKPKLQRWLDEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRG 383
           ++++ +L EEAQ +K + QR++D+  + Y+  ++++S+    I P   K         + 
Sbjct: 293 VAKMAKLVEEAQNSKTETQRFIDKCSKYYTPAIILISICFVAI-PFALK-----VHNLKH 352

Query: 384 SVYRALGLMVAASPCAL-AAAPLAYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTG 443
            V+ AL ++V+A PC L  + P+A   A++  A  G+L+KG   L+ +A    VAFDKTG
Sbjct: 353 WVHLALVVLVSACPCGLILSTPVATFCALTKAATSGLLIKGADYLETLAKIKIVAFDKTG 412

Query: 444 TLTTGGLVFKAIEPIFGHKVGGEKTHLASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVD 503
           T+T G  +    + +       E   L S          L   ++ E  ++HP+  AVVD
Sbjct: 413 TITRGEFIVMDFQSL------SEDISLQS---------LLYWVSSTESKSSHPMAAAVVD 472

Query: 504 H--SAGKDLPSISVESFEYFPGRGLIATLHGIKSGIGGKLRKASLGSVDFITSFCKSENE 563
           +  S   +    +VE ++ FPG G+   + G +  IG K   +  G        C S  +
Sbjct: 473 YARSVSVEPKPEAVEDYQNFPGEGIYGKIDGKEVYIGNKRIASRAG--------CLSVPD 532

Query: 564 SRKIKEAVRASSYGSEFVHAALSVDQKVTLIHLEDQPRPGMLNAISELQDHAKVRVMMLT 623
                  +   + G + +      +    + +L D  R G+  A+ EL+    +++ MLT
Sbjct: 533 -------IDVDTKGGKTIGYVYVGETLAGVFNLSDACRSGVAQAMKELKS-LGIKIAMLT 592

Query: 624 GDHDSSAWKVANAVG--INEVYYSLKPEDKLAHVKEISREMGGGLIMVGEGINDAPALAA 683
           GD+ ++A      +G  ++ V   L PEDK   +K++ RE  G   MVG+G+NDAPALA 
Sbjct: 593 GDNHAAAMHAQEQLGNAMDIVRAELLPEDKSEIIKQLKRE-EGPTAMVGDGLNDAPALAT 652

Query: 684 ATVGIVLARRASATATAVADVLLLQDNISCVPFCIAKSRQTTSLVKQNVTLALSSILLAS 743
           A +GI +    SA AT   +++L+ ++I  +P  I  +++    V +NV ++++      
Sbjct: 653 ADIGISMGVSGSALATETGNIILMSNDIRRIPQAIKLAKRAKRKVVENVVISITMKGAIL 695

Query: 744 LPSVLGFLPLWLTVLLHEGGTLLVCLNSIRALN 771
             +  G   +W  VL   G  LLV LNS+  L+
Sbjct: 713 ALAFAGHPLIWAAVLADVGTCLLVILNSMLLLS 695

BLAST of MC02g1308 vs. TAIR 10
Match: AT5G21930.1 (P-type ATPase of Arabidopsis 2 )

HSP 1 Score: 193.0 bits (489), Expect = 9.5e-49
Identity = 177/586 (30.20%), Postives = 277/586 (47.27%), Query Frame = 0

Query: 192 LLLVMFNLAHIAEEYFTKQSMIDVKELKE-SHPDFALVLDMNDDNLPDTSDLEHR----R 251
           +LL    L    EE    Q+  D+ EL         LV+  +D+N P  S L        
Sbjct: 286 MLLGFVLLGRSLEERAKLQASTDMNELLSLISTQSRLVITSSDNNTPVDSVLSSDSICIN 345

Query: 252 VPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHLTGEVKPLEIKVGERVPGGARNL 311
           V V D+ VG  +LV  GE+ PVD  V  G + +    LTGE  P+  + G  V  G  N 
Sbjct: 346 VSVDDIRVGDSLLVLPGETFPVDGSVLAGRSVVDESMLTGESLPVFKEEGCSVSAGTINW 405

Query: 312 DGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWLDEFGENYSKVVVVLSVAV---- 371
           DG + +KA+ T   ST+S+IV++ E+AQ N   +QR  D     +   ++ LS       
Sbjct: 406 DGPLRIKASSTGSNSTISKIVRMVEDAQGNAAPVQRLADAIAGPFVYTIMSLSAMTFAFW 465

Query: 372 ALIGPLLFKWPFICTPGFRG--------SVYRALGLMVAASPCALA-AAPLAYAIAISSC 431
             +G  +F  P +      G        S+  A+ ++V + PCAL  A P A  I  S  
Sbjct: 466 YYVGSHIF--PDVLLNDIAGPDGDALALSLKLAVDVLVVSCPCALGLATPTAILIGTSLG 525

Query: 432 ARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIFGHKVGGEKTHLASCCI 491
           A++G L++GG VL+ +AS   VA DKTGTLT G  V   +  + G++             
Sbjct: 526 AKRGYLIRGGDVLERLASIDCVALDKTGTLTEGRPVVSGVASL-GYE------------- 585

Query: 492 PSCEKEALAVAAAMEKGTTHPIGRAVVDHSAGKDLPSISVESFEYFPGRGLIATLHGIKS 551
              E+E L +AAA+EK  THPI +A+V+ +   +L +         PG G +A + G   
Sbjct: 586 ---EQEVLKMAAAVEKTATHPIAKAIVNEAESLNLKTPETRGQLTEPGFGTLAEIDG--- 645

Query: 552 GIGGKLRKASLGSVDFITSFCKSENES-----------RKIKEAVRASSYGSEFVHAALS 611
                 R  ++GS+++++     +N+S            K+      S Y    V+    
Sbjct: 646 ------RFVAVGSLEWVSDRFLKKNDSSDMVKLESLLDHKLSNTSSTSRYSKTVVYVGRE 705

Query: 612 VDQKVTLIHLEDQPRPGMLNAISELQDHAKVRVMMLTGDHDSSAWKVANAVGI--NEVYY 671
            +  +  I + D  R      ++ LQ+   ++ ++L+GD + +   VA  VGI      Y
Sbjct: 706 GEGIIGAIAISDCLRQDAEFTVARLQEKG-IKTVLLSGDREGAVATVAKNVGIKSESTNY 765

Query: 672 SLKPEDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLARRASATATA-VADVL 731
           SL PE K   +  + +  G  + MVG+GINDAP+LA A VGI L   A   A +  A V+
Sbjct: 766 SLSPEKKFEFISNL-QSSGHRVAMVGDGINDAPSLAQADVGIALKIEAQENAASNAASVI 825

Query: 732 LLQDNISCVPFCIAKSRQTTSLVKQNVTLALSSILLASLPSVLGFL 746
           L+++ +S V   ++ ++ T S V QN+  A++  ++ S+P   G L
Sbjct: 826 LVRNKLSHVVDALSLAQATMSKVYQNLAWAIAYNVI-SIPIAAGVL 840

BLAST of MC02g1308 vs. TAIR 10
Match: AT5G21930.2 (P-type ATPase of Arabidopsis 2 )

HSP 1 Score: 193.0 bits (489), Expect = 9.5e-49
Identity = 177/586 (30.20%), Postives = 277/586 (47.27%), Query Frame = 0

Query: 192 LLLVMFNLAHIAEEYFTKQSMIDVKELKE-SHPDFALVLDMNDDNLPDTSDLEHR----R 251
           +LL    L    EE    Q+  D+ EL         LV+  +D+N P  S L        
Sbjct: 286 MLLGFVLLGRSLEERAKLQASTDMNELLSLISTQSRLVITSSDNNTPVDSVLSSDSICIN 345

Query: 252 VPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHLTGEVKPLEIKVGERVPGGARNL 311
           V V D+ VG  +LV  GE+ PVD  V  G + +    LTGE  P+  + G  V  G  N 
Sbjct: 346 VSVDDIRVGDSLLVLPGETFPVDGSVLAGRSVVDESMLTGESLPVFKEEGCSVSAGTINW 405

Query: 312 DGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWLDEFGENYSKVVVVLSVAV---- 371
           DG + +KA+ T   ST+S+IV++ E+AQ N   +QR  D     +   ++ LS       
Sbjct: 406 DGPLRIKASSTGSNSTISKIVRMVEDAQGNAAPVQRLADAIAGPFVYTIMSLSAMTFAFW 465

Query: 372 ALIGPLLFKWPFICTPGFRG--------SVYRALGLMVAASPCALA-AAPLAYAIAISSC 431
             +G  +F  P +      G        S+  A+ ++V + PCAL  A P A  I  S  
Sbjct: 466 YYVGSHIF--PDVLLNDIAGPDGDALALSLKLAVDVLVVSCPCALGLATPTAILIGTSLG 525

Query: 432 ARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIFGHKVGGEKTHLASCCI 491
           A++G L++GG VL+ +AS   VA DKTGTLT G  V   +  + G++             
Sbjct: 526 AKRGYLIRGGDVLERLASIDCVALDKTGTLTEGRPVVSGVASL-GYE------------- 585

Query: 492 PSCEKEALAVAAAMEKGTTHPIGRAVVDHSAGKDLPSISVESFEYFPGRGLIATLHGIKS 551
              E+E L +AAA+EK  THPI +A+V+ +   +L +         PG G +A + G   
Sbjct: 586 ---EQEVLKMAAAVEKTATHPIAKAIVNEAESLNLKTPETRGQLTEPGFGTLAEIDG--- 645

Query: 552 GIGGKLRKASLGSVDFITSFCKSENES-----------RKIKEAVRASSYGSEFVHAALS 611
                 R  ++GS+++++     +N+S            K+      S Y    V+    
Sbjct: 646 ------RFVAVGSLEWVSDRFLKKNDSSDMVKLESLLDHKLSNTSSTSRYSKTVVYVGRE 705

Query: 612 VDQKVTLIHLEDQPRPGMLNAISELQDHAKVRVMMLTGDHDSSAWKVANAVGI--NEVYY 671
            +  +  I + D  R      ++ LQ+   ++ ++L+GD + +   VA  VGI      Y
Sbjct: 706 GEGIIGAIAISDCLRQDAEFTVARLQEKG-IKTVLLSGDREGAVATVAKNVGIKSESTNY 765

Query: 672 SLKPEDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLARRASATATA-VADVL 731
           SL PE K   +  + +  G  + MVG+GINDAP+LA A VGI L   A   A +  A V+
Sbjct: 766 SLSPEKKFEFISNL-QSSGHRVAMVGDGINDAPSLAQADVGIALKIEAQENAASNAASVI 825

Query: 732 LLQDNISCVPFCIAKSRQTTSLVKQNVTLALSSILLASLPSVLGFL 746
           L+++ +S V   ++ ++ T S V QN+  A++  ++ S+P   G L
Sbjct: 826 LVRNKLSHVVDALSLAQATMSKVYQNLAWAIAYNVI-SIPIAAGVL 840