MC02g1017 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC02g1017
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionInositol-tetrakisphosphate 1-kinase
LocationMC02: 8185787 .. 8186803 (+)
RNA-Seq ExpressionMC02g1017
SyntenyMC02g1017
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GCAGCCATGGCTTCTTCTTCGGAGCGCCTTCGAATCGGCTACGCTTTCCCCCCGAACAAAGAGCACAATGTCATTCGGCCGTCTTTGATTGACTACGCGAAGCTCCACGGCGTCGACCTGGTCCGCATCGACCTTCAAACGCCGCTCCTCCACCAAGCTCCCTTCCACTGCATCATCCACAAGCTCTACGACGACGCTTGGGCGGAGCATCTGCAGGAGTTCGCGTCGAAGAATCCGGACGTCGTTGTCCTGGAACAGCCGGAGCTGATCTCGCGGCTCAACGACAGAGTCTCGATGCTGGATGTGGTGAAGGAGGTGAAGGTTTCGGAGAGCGGCGTGAAGATCGAGGTACCGAAACAGGTAATTCTGAACGAATTGGAGGATCCGATTAGGGATTTGGAGGTGAAATTTCCTGTGATTGCGAAGCCGTTGAGGTCGGATGGAAGTGCGCAGTCGCACGAAATGTCTCTGGTGTTCAATCGGCGCGGATTGAAGGAACTGGAATCGCCGGTGCTGCTGCAGGAATTTGTGAACCACGGCGGAGTTATGTTCAAGGTTTACGTGATCGGAGAGGAATCGGTGTGCGTGAAGCGGAAATCGCTGCCGGATGTAGCGGAGACAGAGGAGGAGCTGGAGGAGAAAGCAGAGGGCGCGATGAAATTTTCTCAGATATCGAGGGCGGAGAAGCAGGACAAGCACGGCGGAGGCGGCGGCGGCGGCGGAGAGAAGGAGGAGGAGATCGAAATGCCTTCGTCGAAGCTAGTGGCGGAGGTATCGAGAGGGCTGAGGGAAGCGATGGGGCTGCGGCTGTTCAATTTCGACATGATTAGGGATCGGAACGGCAACTACTTCGTGATCGACATAAATTACCTGCCAGGATTTGCAGTACTGCCGAATTACGAGCCGTTTCTGACGAAATTTTTGAAGGAAGTTGCGGAGAGGAAGAAGAAGAAGGCGGAAGCGGACGCCATTGGAGACGCTGAACCTGCAGAACCGAAACCTCGATGCTGCTGTTTC

mRNA sequence

GCAGCCATGGCTTCTTCTTCGGAGCGCCTTCGAATCGGCTACGCTTTCCCCCCGAACAAAGAGCACAATGTCATTCGGCCGTCTTTGATTGACTACGCGAAGCTCCACGGCGTCGACCTGGTCCGCATCGACCTTCAAACGCCGCTCCTCCACCAAGCTCCCTTCCACTGCATCATCCACAAGCTCTACGACGACGCTTGGGCGGAGCATCTGCAGGAGTTCGCGTCGAAGAATCCGGACGTCGTTGTCCTGGAACAGCCGGAGCTGATCTCGCGGCTCAACGACAGAGTCTCGATGCTGGATGTGGTGAAGGAGGTGAAGGTTTCGGAGAGCGGCGTGAAGATCGAGGTACCGAAACAGGTAATTCTGAACGAATTGGAGGATCCGATTAGGGATTTGGAGGTGAAATTTCCTGTGATTGCGAAGCCGTTGAGGTCGGATGGAAGTGCGCAGTCGCACGAAATGTCTCTGGTGTTCAATCGGCGCGGATTGAAGGAACTGGAATCGCCGGTGCTGCTGCAGGAATTTGTGAACCACGGCGGAGTTATGTTCAAGGTTTACGTGATCGGAGAGGAATCGGTGTGCGTGAAGCGGAAATCGCTGCCGGATGTAGCGGAGACAGAGGAGGAGCTGGAGGAGAAAGCAGAGGGCGCGATGAAATTTTCTCAGATATCGAGGGCGGAGAAGCAGGACAAGCACGGCGGAGGCGGCGGCGGCGGCGGAGAGAAGGAGGAGGAGATCGAAATGCCTTCGTCGAAGCTAGTGGCGGAGGTATCGAGAGGGCTGAGGGAAGCGATGGGGCTGCGGCTGTTCAATTTCGACATGATTAGGGATCGGAACGGCAACTACTTCGTGATCGACATAAATTACCTGCCAGGATTTGCAGTACTGCCGAATTACGAGCCGTTTCTGACGAAATTTTTGAAGGAAGTTGCGGAGAGGAAGAAGAAGAAGGCGGAAGCGGACGCCATTGGAGACGCTGAACCTGCAGAACCGAAACCTCGATGCTGCTGTTTC

Coding sequence (CDS)

GCAGCCATGGCTTCTTCTTCGGAGCGCCTTCGAATCGGCTACGCTTTCCCCCCGAACAAAGAGCACAATGTCATTCGGCCGTCTTTGATTGACTACGCGAAGCTCCACGGCGTCGACCTGGTCCGCATCGACCTTCAAACGCCGCTCCTCCACCAAGCTCCCTTCCACTGCATCATCCACAAGCTCTACGACGACGCTTGGGCGGAGCATCTGCAGGAGTTCGCGTCGAAGAATCCGGACGTCGTTGTCCTGGAACAGCCGGAGCTGATCTCGCGGCTCAACGACAGAGTCTCGATGCTGGATGTGGTGAAGGAGGTGAAGGTTTCGGAGAGCGGCGTGAAGATCGAGGTACCGAAACAGGTAATTCTGAACGAATTGGAGGATCCGATTAGGGATTTGGAGGTGAAATTTCCTGTGATTGCGAAGCCGTTGAGGTCGGATGGAAGTGCGCAGTCGCACGAAATGTCTCTGGTGTTCAATCGGCGCGGATTGAAGGAACTGGAATCGCCGGTGCTGCTGCAGGAATTTGTGAACCACGGCGGAGTTATGTTCAAGGTTTACGTGATCGGAGAGGAATCGGTGTGCGTGAAGCGGAAATCGCTGCCGGATGTAGCGGAGACAGAGGAGGAGCTGGAGGAGAAAGCAGAGGGCGCGATGAAATTTTCTCAGATATCGAGGGCGGAGAAGCAGGACAAGCACGGCGGAGGCGGCGGCGGCGGCGGAGAGAAGGAGGAGGAGATCGAAATGCCTTCGTCGAAGCTAGTGGCGGAGGTATCGAGAGGGCTGAGGGAAGCGATGGGGCTGCGGCTGTTCAATTTCGACATGATTAGGGATCGGAACGGCAACTACTTCGTGATCGACATAAATTACCTGCCAGGATTTGCAGTACTGCCGAATTACGAGCCGTTTCTGACGAAATTTTTGAAGGAAGTTGCGGAGAGGAAGAAGAAGAAGGCGGAAGCGGACGCCATTGGAGACGCTGAACCTGCAGAACCGAAACCTCGATGCTGCTGTTTC

Protein sequence

AAMASSSERLRIGYAFPPNKEHNVIRPSLIDYAKLHGVDLVRIDLQTPLLHQAPFHCIIHKLYDDAWAEHLQEFASKNPDVVVLEQPELISRLNDRVSMLDVVKEVKVSESGVKIEVPKQVILNELEDPIRDLEVKFPVIAKPLRSDGSAQSHEMSLVFNRRGLKELESPVLLQEFVNHGGVMFKVYVIGEESVCVKRKSLPDVAETEEELEEKAEGAMKFSQISRAEKQDKHGGGGGGGGEKEEEIEMPSSKLVAEVSRGLREAMGLRLFNFDMIRDRNGNYFVIDINYLPGFAVLPNYEPFLTKFLKEVAERKKKKAEADAIGDAEPAEPKPRCCCF
Homology
BLAST of MC02g1017 vs. ExPASy Swiss-Prot
Match: Q9SBA5 (Inositol-tetrakisphosphate 1-kinase 1 OS=Arabidopsis thaliana OX=3702 GN=ITPK1 PE=1 SV=1)

HSP 1 Score: 266.9 bits (681), Expect = 3.1e-70
Identity = 147/318 (46.23%), Postives = 216/318 (67.92%), Query Frame = 0

Query: 5   SSSERLRIGYAFPPNKEHNVIRPSLIDYAKLHGVDLVRIDLQTPLLHQAPFHCIIHKLYD 64
           S  ER  +GYA    K+H+ I+PSLI++++  G+DLV++D    LL Q    CIIHKLYD
Sbjct: 4   SIQERYLVGYALAAKKQHSFIQPSLIEHSRQRGIDLVKLDPTKSLLEQGKLDCIIHKLYD 63

Query: 65  DAWAEHLQEFASKNPDVVVLEQPELISRLNDRVSMLDVVKEVK--VSESGVKIEVPKQVI 124
             W E+L EF  K P V V++ PE I RL++RVSML+V+ +++  VS+S  +  VP+QV+
Sbjct: 64  VYWKENLHEFREKCPGVPVIDLPEAIERLHNRVSMLEVITQLRFPVSDS-ERFGVPEQVV 123

Query: 125 LNE---LEDPIRDLEVKFPVIAKPLRSDGSAQSHEMSLVFNRRGLKELESPVLLQEFVNH 184
           + +   L       E+KFPVIAKPL +DGSA+SH+M L++++ G+K L++P++LQEFVNH
Sbjct: 124 VMDSSVLSGGGALGELKFPVIAKPLDADGSAKSHKMFLIYDQEGMKILKAPIVLQEFVNH 183

Query: 185 GGVMFKVYVIGEESVCVKRKSLPDVAETEEELEEKAEGAMKFSQISRAEKQDKHGGGGGG 244
           GGV+FKVYV+G+   CVKR+SLPD++   EE    ++G++ FSQIS    Q+       G
Sbjct: 184 GGVIFKVYVVGDHVKCVKRRSLPDIS---EEKIGTSKGSLPFSQISNLTAQEDK-NIEYG 243

Query: 245 GGEKEEEIEMPSSKLVAEVSRGLREAMGLRLFNFDMIRDRN--GNYFVIDINYLPGFAVL 304
                E++EMP    + ++++ +RE+MGL LFNFD+IRD      Y +IDINY PG+A +
Sbjct: 244 EDRSLEKVEMPPLSFLTDLAKAMRESMGLNLFNFDVIRDAKDANRYLIIDINYFPGYAKM 303

Query: 305 PNYEPFLTKFLKEVAERK 316
           P+YEP LT+F  ++  +K
Sbjct: 304 PSYEPVLTEFFWDMVTKK 316

BLAST of MC02g1017 vs. ExPASy Swiss-Prot
Match: Q84Y01 (Inositol-tetrakisphosphate 1-kinase 1 OS=Zea mays OX=4577 GN=ITPK1 PE=2 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 5.1e-65
Identity = 141/312 (45.19%), Postives = 201/312 (64.42%), Query Frame = 0

Query: 9   RLRIGYAFPPNKEHNVIRPSLIDYAKLHGVDLVRIDLQTPLLHQAPFHCIIHKLYDDAWA 68
           R  IGYA  P K+ + I+PSL+  A   G+DLV +D   PL  Q PFH +IHKLY D W 
Sbjct: 18  RYVIGYALAPKKQQSFIQPSLVAQAASRGMDLVPVDASQPLAEQGPFHLLIHKLYGDDWR 77

Query: 69  EHLQEFASKNPDVVVLEQPELISRLNDRVSMLDVVKEV-KVSESGVKIEVPKQVILNE-- 128
             L  FA+++P V +++ P  I RL++R+SML VV E+   ++      +P QV++ +  
Sbjct: 78  AQLVAFAARHPAVPIVDPPHAIDRLHNRISMLQVVSELDHAADQDSTFGIPSQVVVYDAA 137

Query: 129 -LEDPIRDLEVKFPVIAKPLRSDGSAQSHEMSLVFNRRGLKELESPVLLQEFVNHGGVMF 188
            L D      ++FP+IAKPL +DG+A+SH+MSLV++R GL +L  P++LQEFVNHGGV+F
Sbjct: 138 ALADFGLLAALRFPLIAKPLVADGTAKSHKMSLVYHREGLGKLRPPLVLQEFVNHGGVIF 197

Query: 189 KVYVIGEESVCVKRKSLPDVAETEEELEEKAEGAMKFSQISRAEKQDKHGGGGGGGGEKE 248
           KVYV+G    CVKR+SLPDV+    E +  A+G++ FSQ+S    +         G +  
Sbjct: 198 KVYVVGGHVTCVKRRSLPDVS---PEDDASAQGSVSFSQVSNLPTE--RTAEEYYGEKSL 257

Query: 249 EEIEMPSSKLVAEVSRGLREAMGLRLFNFDMIRD-RNGN-YFVIDINYLPGFAVLPNYEP 308
           E+  +P +  + +++ GLR A+GL+LFNFDMIRD R G+ Y VIDINY PG+A +P YE 
Sbjct: 258 EDAVVPPAAFINQIAGGLRRALGLQLFNFDMIRDVRAGDRYLVIDINYFPGYAKMPGYET 317

Query: 309 FLTKFLKEVAER 315
            LT F  E+  +
Sbjct: 318 VLTDFFWEMVHK 324

BLAST of MC02g1017 vs. ExPASy Swiss-Prot
Match: A2X4M8 (Inositol-tetrakisphosphate 1-kinase 4 OS=Oryza sativa subsp. indica OX=39946 GN=ITPK4 PE=3 SV=1)

HSP 1 Score: 245.4 bits (625), Expect = 9.5e-64
Identity = 141/317 (44.48%), Postives = 197/317 (62.15%), Query Frame = 0

Query: 5   SSSERLRIGYAFPPNKEHNVIRPSLIDYAKLHGVDLVRIDLQTPLLHQAPFHCIIHKLYD 64
           SSS R  +GYA  P K  +V+RPSL+  A   GV LV +D+  PL  Q PF  ++HK+YD
Sbjct: 9   SSSPRYTVGYALLPEKVSSVVRPSLVALAADRGVRLVAVDVSRPLAEQGPFDLLVHKMYD 68

Query: 65  DAWAEHLQEFASKNPDV-VVLEQPELISRLNDRVSMLDVVKEVKVSESGVKIEVPKQVIL 124
             W   L+E A+++P V VV++ P  I RL DR +MLDVV  ++       + VP QV++
Sbjct: 69  RGWRAQLEELAARHPGVPVVVDSPGAIDRLLDRATMLDVVSGLR-----TPVSVPPQVVV 128

Query: 125 N----ELEDPIRDLEVKFPVIAKPLRSDGSAQSHEMSLVFNRRG-LKELESPVLLQEFVN 184
           +    + ++ +    ++FP+IAKPL  DGSA+SH+M LV+ R G L  L +P++LQEFVN
Sbjct: 129 SDAAADADELLARAALRFPLIAKPLAVDGSAESHDMRLVYRRDGVLPLLRAPLVLQEFVN 188

Query: 185 HGGVMFKVYVIGEESVCVKRKSLPDVAETEEELEEKAEGAMKFSQISRAEKQDKHGGGGG 244
           HGGV+FKVYV+G+ + CV+R SLPDV      L+  AE ++ F+ IS          GG 
Sbjct: 189 HGGVLFKVYVVGDRATCVRRSSLPDV-PARRLLDLDAEPSVPFANISNQPLPPPDDDGGA 248

Query: 245 GGGEKEEEIEMPSSKLVAEVSRGLREAMGLRLFNFDMIRDRN----GNYFVIDINYLPGF 304
                  + + P++  V EV+RGLR  +GL LFNFDMIR+R+      YF+IDINY PG+
Sbjct: 249 A------DDDTPAAGFVDEVARGLRRGLGLHLFNFDMIRERSEEHGDRYFIIDINYFPGY 308

Query: 305 AVLPNYEPFLTKFLKEV 312
           A +P YE  LT F  E+
Sbjct: 309 AKMPGYEAALTDFFLEM 313

BLAST of MC02g1017 vs. ExPASy Swiss-Prot
Match: Q6K7B8 (Inositol-tetrakisphosphate 1-kinase 4 OS=Oryza sativa subsp. japonica OX=39947 GN=ITPK4 PE=2 SV=1)

HSP 1 Score: 245.4 bits (625), Expect = 9.5e-64
Identity = 141/317 (44.48%), Postives = 197/317 (62.15%), Query Frame = 0

Query: 5   SSSERLRIGYAFPPNKEHNVIRPSLIDYAKLHGVDLVRIDLQTPLLHQAPFHCIIHKLYD 64
           SSS R  +GYA  P K  +V+RPSL+  A   GV LV +D+  PL  Q PF  ++HK+YD
Sbjct: 9   SSSPRYTVGYALLPEKVSSVVRPSLVALAADRGVRLVAVDVSRPLAEQGPFDLLVHKMYD 68

Query: 65  DAWAEHLQEFASKNPDV-VVLEQPELISRLNDRVSMLDVVKEVKVSESGVKIEVPKQVIL 124
             W   L+E A+++P V VV++ P  I RL DR +MLDVV  ++       + VP QV++
Sbjct: 69  RGWRAQLEELAARHPGVTVVVDSPGAIDRLLDRATMLDVVSGLR-----TPVSVPPQVVV 128

Query: 125 N----ELEDPIRDLEVKFPVIAKPLRSDGSAQSHEMSLVFNRRG-LKELESPVLLQEFVN 184
           +    + ++ +    ++FP+IAKPL  DGSA+SH+M LV+ R G L  L +P++LQEFVN
Sbjct: 129 SDAAADADELLARAALRFPLIAKPLAVDGSAESHDMRLVYRRDGVLPLLRAPLVLQEFVN 188

Query: 185 HGGVMFKVYVIGEESVCVKRKSLPDVAETEEELEEKAEGAMKFSQISRAEKQDKHGGGGG 244
           HGGV+FKVYV+G+ + CV+R SLPDV      L+  AE ++ F+ IS          GG 
Sbjct: 189 HGGVLFKVYVVGDRATCVRRSSLPDV-PARRLLDLDAEPSVPFANISNQPLPPPDDDGGA 248

Query: 245 GGGEKEEEIEMPSSKLVAEVSRGLREAMGLRLFNFDMIRDRN----GNYFVIDINYLPGF 304
                  + + P++  V EV+RGLR  +GL LFNFDMIR+R+      YF+IDINY PG+
Sbjct: 249 A------DDDTPAAGFVDEVARGLRRGLGLHLFNFDMIRERSEEHGDRYFIIDINYFPGY 308

Query: 305 AVLPNYEPFLTKFLKEV 312
           A +P YE  LT F  E+
Sbjct: 309 AKMPGYEAALTDFFLEM 313

BLAST of MC02g1017 vs. ExPASy Swiss-Prot
Match: A0JJZ6 (Inositol-tetrakisphosphate 1-kinase 5 OS=Oryza sativa subsp. indica OX=39946 GN=ITPK5 PE=3 SV=1)

HSP 1 Score: 245.0 bits (624), Expect = 1.2e-63
Identity = 138/314 (43.95%), Postives = 197/314 (62.74%), Query Frame = 0

Query: 9   RLRIGYAFPPNKEHNVIRPSLIDYAKLHGVDLVRIDLQTPLLHQAPFHCIIHKLYDDAWA 68
           R  IGYA  P K+ + I+PSL+  A   G+DLV +D   PL  Q PFH +IHKLY + W 
Sbjct: 15  RYLIGYALAPKKQQSFIQPSLVSRAAGRGMDLVPVDPSRPLPEQGPFHLLIHKLYGEEWR 74

Query: 69  EHLQEFASKNPDVVVLEQPELISRLNDRVSMLDVVKEVKV---SESGVKIEVPKQVILNE 128
             L  F++ +P V V++ P  I RL++R+SML VV E+ V   +       +P QV++ +
Sbjct: 75  GQLDAFSAAHPAVPVVDPPHAIDRLHNRISMLQVVSELDVPLHAHHHHTFGIPSQVVVYD 134

Query: 129 ---LEDPIRDLEVKFPVIAKPLRSDGSAQSHEMSLVFNRRGLKELESPVLLQEFVNHGGV 188
              L D      ++FP+IAKPL +DG+A+SH+MSLV++R GL++L  P++LQEFVNHGGV
Sbjct: 135 AAALSDSGLLAALRFPLIAKPLVADGTAKSHKMSLVYHREGLRKLRPPLVLQEFVNHGGV 194

Query: 189 MFKVYVIGEESVCVKRKSLPDVAETEEELEEKAEGAMKFSQISRAEKQDKHGGGGGGGGE 248
           +FKVYV+G    CVKR+SLPDV+ ++   +  AEG++ FSQ+S     ++          
Sbjct: 195 IFKVYVVGAHVTCVKRRSLPDVS-SDVLQDASAEGSLSFSQVSNL--PNERTAQEYYDDM 254

Query: 249 KEEEIEMPSSKLVAEVSRGLREAMGLRLFNFDMIRDRNG--NYFVIDINYLPGFAVLPNY 308
           + E+  MP +  + +++  LR A+GL LFNFDMIRD      Y VIDINY PG+A +P Y
Sbjct: 255 RLEDAIMPPTAFINDIAAALRRALGLHLFNFDMIRDARAGDRYLVIDINYFPGYAKMPGY 314

Query: 309 EPFLTKFLKEVAER 315
           E  LT F  E+  +
Sbjct: 315 ETVLTDFFWEMVHK 325

BLAST of MC02g1017 vs. NCBI nr
Match: XP_022986561.1 (inositol-tetrakisphosphate 1-kinase 1-like [Cucurbita maxima])

HSP 1 Score: 514 bits (1323), Expect = 7.43e-181
Identity = 263/344 (76.45%), Postives = 298/344 (86.63%), Query Frame = 0

Query: 1   AAMASSSERLRIGYAFPPNKEHNVIRPSLIDYAKLHGVDLVRIDLQTPLLHQAPFHCIIH 60
           ++ +SSS RLR+GYAFPPNKE NVIRPSL+DYAKLHGVDLVRID+QTPLLHQ PFHCIIH
Sbjct: 3   SSSSSSSNRLRVGYAFPPNKERNVIRPSLVDYAKLHGVDLVRIDIQTPLLHQGPFHCIIH 62

Query: 61  KLYDDAWAEHLQEFASKNPDVVVLEQPELISRLNDRVSMLDVVKEVKVSESGVKIEVPKQ 120
           KLYDDAWAEHLQ+FASKNPDVVV+++P+LI++L DRVSMLDVV +VKVS+SGV+IEVPKQ
Sbjct: 63  KLYDDAWAEHLQDFASKNPDVVVVDRPDLIAQLYDRVSMLDVVSQVKVSDSGVRIEVPKQ 122

Query: 121 VILNELE---DPIRDLEVKFPVIAKPLRSDGSAQSHEMSLVFNRRGLKELESPVLLQEFV 180
           ++L + +   D IRDL+VKFPVIAKPL SDGSA+SHEMSLVFNRRGLK+LE+PVLLQEFV
Sbjct: 123 IVLKQEDGEIDSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDLETPVLLQEFV 182

Query: 181 NHGGVMFKVYVIGEESVCVKRKSLPDVAETEEELEEKAEGAMKFSQISRAEKQDKHGGGG 240
           NHGGVMFKVYV GEESVCVKRKSLPDV ETEEELE+K +GAMKFSQISRAE++ +   G 
Sbjct: 183 NHGGVMFKVYVAGEESVCVKRKSLPDVTETEEELEKKPDGAMKFSQISRAEEKSEKCDGA 242

Query: 241 G--GGGEKEEEIEMPSSKLVAEVSRGLREAMGLRLFNFDMIRDRNGNYFVIDINYLPGFA 300
                 EKEEEIEMP  K+V EVS GL+EAMG+RLFNFDMIRD NG Y+VIDINYLPGFA
Sbjct: 243 EKKAAAEKEEEIEMPPEKIVREVSNGLKEAMGIRLFNFDMIRDGNGTYYVIDINYLPGFA 302

Query: 301 VLPNYEPFLTKFLKEVAERKKKKAEADAIGDAEPAEPKPRCCCF 339
           VLP YE FLTKF KEVAE+KKKK E D     E  + KPRCCCF
Sbjct: 303 VLPEYEQFLTKFFKEVAEKKKKKVEEDDSA-IEDGKAKPRCCCF 345

BLAST of MC02g1017 vs. NCBI nr
Match: XP_023512946.1 (inositol-tetrakisphosphate 1-kinase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 514 bits (1323), Expect = 8.28e-181
Identity = 262/344 (76.16%), Postives = 298/344 (86.63%), Query Frame = 0

Query: 1   AAMASSSERLRIGYAFPPNKEHNVIRPSLIDYAKLHGVDLVRIDLQTPLLHQAPFHCIIH 60
           ++ +SSS RLR+GYAFPPNKE NVIRPSL+DYAKLHGVDLVRIDLQTPLLHQ PFHCIIH
Sbjct: 5   SSSSSSSNRLRVGYAFPPNKERNVIRPSLVDYAKLHGVDLVRIDLQTPLLHQGPFHCIIH 64

Query: 61  KLYDDAWAEHLQEFASKNPDVVVLEQPELISRLNDRVSMLDVVKEVKVSESGVKIEVPKQ 120
           KLYDDAWAEHLQ+FASKNPDVVV+++P+LI+RL DRVSMLDVV +VK S+SGV+IEVPKQ
Sbjct: 65  KLYDDAWAEHLQDFASKNPDVVVVDRPDLIARLYDRVSMLDVVSQVKASDSGVRIEVPKQ 124

Query: 121 VILNELE---DPIRDLEVKFPVIAKPLRSDGSAQSHEMSLVFNRRGLKELESPVLLQEFV 180
           ++L + +   D IRDL+VKFPVIAKPL SDGSA+SHEMSLVFNRRGLK+LE+PVLLQEFV
Sbjct: 125 IVLKQEDGEIDSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDLETPVLLQEFV 184

Query: 181 NHGGVMFKVYVIGEESVCVKRKSLPDVAETEEELEEKAEGAMKFSQISRAEKQDK--HGG 240
           NHGGVMFKVYV GEESVCVKRKSLPDVAETEEELE+K +GAMKFSQISRAE++ +  +G 
Sbjct: 185 NHGGVMFKVYVAGEESVCVKRKSLPDVAETEEELEKKPDGAMKFSQISRAEEKSEKCNGA 244

Query: 241 GGGGGGEKEEEIEMPSSKLVAEVSRGLREAMGLRLFNFDMIRDRNGNYFVIDINYLPGFA 300
                 E+EEEIEMP  K+V EVS GLREAMG+RLFNFDMIRD NG Y+VIDINYLPGFA
Sbjct: 245 EKKAAAEEEEEIEMPPEKIVREVSNGLREAMGIRLFNFDMIRDGNGTYYVIDINYLPGFA 304

Query: 301 VLPNYEPFLTKFLKEVAERKKKKAEADAIGDAEPAEPKPRCCCF 339
           VLP YE FLTKF KEVAE++KKK   +     E  + KPRCCCF
Sbjct: 305 VLPEYEQFLTKFFKEVAEKEKKKKVEEDDSAIEDGKAKPRCCCF 348

BLAST of MC02g1017 vs. NCBI nr
Match: KAG6571172.1 (Inositol-tetrakisphosphate 1-kinase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 508 bits (1309), Expect = 9.34e-179
Identity = 262/344 (76.16%), Postives = 296/344 (86.05%), Query Frame = 0

Query: 1   AAMASSSERLRIGYAFPPNKEHNVIRPSLIDYAKLHGVDLVRIDLQTPLLHQAPFHCIIH 60
           ++ +SSS RLR+GYAFPPNKE NVIRPSL+DYAKLHGVDLVRIDLQTPLLHQ PFHCIIH
Sbjct: 4   SSSSSSSNRLRVGYAFPPNKERNVIRPSLVDYAKLHGVDLVRIDLQTPLLHQGPFHCIIH 63

Query: 61  KLYDDAWAEHLQEFASKNPDVVVLEQPELISRLNDRVSMLDVVKEVKVSESGVKIEVPKQ 120
           KLYDDAWAEHLQ+FASKNPDVVV+++P+LI+RL DRVSMLDVV +VK S+SGV+IEVPKQ
Sbjct: 64  KLYDDAWAEHLQDFASKNPDVVVVDRPDLIARLYDRVSMLDVVSQVKGSDSGVRIEVPKQ 123

Query: 121 VILNELE---DPIRDLEVKFPVIAKPLRSDGSAQSHEMSLVFNRRGLKELESPVLLQEFV 180
           ++L + +   D IRDL+VKFPVIAKPL SDGSA+SHEMSLVFNRRGLK+L+ PVLLQEFV
Sbjct: 124 IVLKQEDGEIDSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDLDRPVLLQEFV 183

Query: 181 NHGGVMFKVYVIGEESVCVKRKSLPDVAETEEELEEKAEGAMKFSQISRAEKQDKHGGGG 240
           NHGGVMFKVYV GEESVCVKRKSLPDVAETEEELE+K +GAMKFSQISRAE++ +   G 
Sbjct: 184 NHGGVMFKVYVAGEESVCVKRKSLPDVAETEEELEKKQDGAMKFSQISRAEEKSEKCNGA 243

Query: 241 GGGGEKEE--EIEMPSSKLVAEVSRGLREAMGLRLFNFDMIRDRNGNYFVIDINYLPGFA 300
               EK+E  EIEMP  K+V EVS GLREAMG+RLFNFDMIRD NG Y+VIDINYLPGFA
Sbjct: 244 ----EKKEAEEIEMPPEKIVREVSNGLREAMGIRLFNFDMIRDGNGTYYVIDINYLPGFA 303

Query: 301 VLPNYEPFLTKFLKEVAERKKKKAEADAIGDAEPAEPKPRCCCF 339
           VLP YE FLTKF KEVAE+KKKK   +     E  + KPRCCCF
Sbjct: 304 VLPEYEQFLTKFFKEVAEKKKKKKVEEDDSAIEDGKAKPRCCCF 343

BLAST of MC02g1017 vs. NCBI nr
Match: XP_022943687.1 (inositol-tetrakisphosphate 1-kinase 1-like [Cucurbita moschata])

HSP 1 Score: 507 bits (1306), Expect = 2.58e-178
Identity = 261/344 (75.87%), Postives = 295/344 (85.76%), Query Frame = 0

Query: 1   AAMASSSERLRIGYAFPPNKEHNVIRPSLIDYAKLHGVDLVRIDLQTPLLHQAPFHCIIH 60
           ++ +SSS RLR+GYAFPPNKE NVIRPSL+DYAKLHGVDLVRIDLQTPLLHQ PFHCIIH
Sbjct: 3   SSSSSSSNRLRVGYAFPPNKERNVIRPSLVDYAKLHGVDLVRIDLQTPLLHQGPFHCIIH 62

Query: 61  KLYDDAWAEHLQEFASKNPDVVVLEQPELISRLNDRVSMLDVVKEVKVSESGVKIEVPKQ 120
           KLYDDAWAEHLQ+FA KNPDVVV+++P+LI+RL DRVSMLDVV +VK S+SGV+IEVPKQ
Sbjct: 63  KLYDDAWAEHLQDFALKNPDVVVVDRPDLIARLYDRVSMLDVVSQVKASDSGVRIEVPKQ 122

Query: 121 VILNELE---DPIRDLEVKFPVIAKPLRSDGSAQSHEMSLVFNRRGLKELESPVLLQEFV 180
           ++L + +   D IRDL+VKFPVIAKPL SDGSA+SHEMSLVFNRRGLK+L+ PVLLQEFV
Sbjct: 123 IVLKQEDGEIDSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDLDRPVLLQEFV 182

Query: 181 NHGGVMFKVYVIGEESVCVKRKSLPDVAETEEELEEKAEGAMKFSQISRAEKQDKHGGGG 240
           NHGGVMFKVYV GEESVCVKRKSLPDVAETEEELE+K +GAMKFSQISRAE++ +   G 
Sbjct: 183 NHGGVMFKVYVAGEESVCVKRKSLPDVAETEEELEKKQDGAMKFSQISRAEEKSEKCNGA 242

Query: 241 GGGGEKEE--EIEMPSSKLVAEVSRGLREAMGLRLFNFDMIRDRNGNYFVIDINYLPGFA 300
               EK+E  EIEMP  K+V EVS GLREAMG+RLFNFDMIRD NG Y+VIDINYLPGFA
Sbjct: 243 ----EKKEAEEIEMPPEKIVREVSNGLREAMGIRLFNFDMIRDGNGTYYVIDINYLPGFA 302

Query: 301 VLPNYEPFLTKFLKEVAERKKKKAEADAIGDAEPAEPKPRCCCF 339
           VLP YE FLTKF KEVAE+KKKK   +     E  + KPRCCCF
Sbjct: 303 VLPEYEQFLTKFFKEVAEKKKKKKVEEDDSAIEDGKAKPRCCCF 342

BLAST of MC02g1017 vs. NCBI nr
Match: XP_023007496.1 (inositol-tetrakisphosphate 1-kinase 1-like [Cucurbita maxima])

HSP 1 Score: 493 bits (1269), Expect = 2.60e-172
Identity = 255/343 (74.34%), Postives = 291/343 (84.84%), Query Frame = 0

Query: 1   AAMASSSERLRIGYAFPPNKEHNVIRPSLIDYAKLHGVDLVRIDLQTPLLHQAPFHCIIH 60
           ++ +SSS+  R+GYAFPPNKE NVIRPSLIDYAKLHGVDLVRIDLQTP+LHQ PFHCIIH
Sbjct: 8   SSSSSSSDGFRVGYAFPPNKERNVIRPSLIDYAKLHGVDLVRIDLQTPILHQGPFHCIIH 67

Query: 61  KLYDDAWAEHLQEFASKNPDVVVLEQPELISRLNDRVSMLDVVKEVKVSESGVKIEVPKQ 120
           K+YDDAW + LQ+FASKNPDVVV+++P+ I RL +RVSMLDVV +VKVS+SGVKIEVPKQ
Sbjct: 68  KMYDDAWVDTLQDFASKNPDVVVVDRPDRIERLYNRVSMLDVVNQVKVSDSGVKIEVPKQ 127

Query: 121 VILNELE---DPIRDLEVKFPVIAKPLRSDGSAQSHEMSLVFNRRGLKELESPVLLQEFV 180
           V+LN  +   D IRDLEVKFPVIAKPL S+GSA+SHEMSLV+NRRG K+LE+PVLLQEFV
Sbjct: 128 VVLNRKDGVIDLIRDLEVKFPVIAKPLESNGSAKSHEMSLVYNRRGFKDLETPVLLQEFV 187

Query: 181 NHGGVMFKVYVIGEESVCVKRKSLPDVAETEEELEEKAEGAMKFSQISRAE-KQDKHGGG 240
           NHGGVMFKVYV GE+SVCVKRKSLPDV ETEEELE+K +GAMKFSQISRAE K +K   G
Sbjct: 188 NHGGVMFKVYVAGEQSVCVKRKSLPDVVETEEELEKKTDGAMKFSQISRAEEKSEKCNCG 247

Query: 241 GGGGGEKEEEIEMPSSKLVAEVSRGLREAMGLRLFNFDMIRDRNGNYFVIDINYLPGFAV 300
           G      EEEIEMP  K+V EVSRGLREAMG+RLFNFDMIRDRNG Y+VIDINYLPGFAV
Sbjct: 248 GKKEAAAEEEIEMPPEKVVTEVSRGLREAMGIRLFNFDMIRDRNGTYYVIDINYLPGFAV 307

Query: 301 LPNYEPFLTKFLKEVAERKKKKAEADAIGDAEPAEPKPRCCCF 339
           LP+YE FLTKF  EV ++K  + E  A+ D    + KPRCCCF
Sbjct: 308 LPDYEAFLTKFFNEVMQKKVVEEENSAMEDE---KAKPRCCCF 347

BLAST of MC02g1017 vs. ExPASy TrEMBL
Match: A0A6J1JBH8 (Inositol-tetrakisphosphate 1-kinase OS=Cucurbita maxima OX=3661 GN=LOC111484264 PE=3 SV=1)

HSP 1 Score: 514 bits (1323), Expect = 3.60e-181
Identity = 263/344 (76.45%), Postives = 298/344 (86.63%), Query Frame = 0

Query: 1   AAMASSSERLRIGYAFPPNKEHNVIRPSLIDYAKLHGVDLVRIDLQTPLLHQAPFHCIIH 60
           ++ +SSS RLR+GYAFPPNKE NVIRPSL+DYAKLHGVDLVRID+QTPLLHQ PFHCIIH
Sbjct: 3   SSSSSSSNRLRVGYAFPPNKERNVIRPSLVDYAKLHGVDLVRIDIQTPLLHQGPFHCIIH 62

Query: 61  KLYDDAWAEHLQEFASKNPDVVVLEQPELISRLNDRVSMLDVVKEVKVSESGVKIEVPKQ 120
           KLYDDAWAEHLQ+FASKNPDVVV+++P+LI++L DRVSMLDVV +VKVS+SGV+IEVPKQ
Sbjct: 63  KLYDDAWAEHLQDFASKNPDVVVVDRPDLIAQLYDRVSMLDVVSQVKVSDSGVRIEVPKQ 122

Query: 121 VILNELE---DPIRDLEVKFPVIAKPLRSDGSAQSHEMSLVFNRRGLKELESPVLLQEFV 180
           ++L + +   D IRDL+VKFPVIAKPL SDGSA+SHEMSLVFNRRGLK+LE+PVLLQEFV
Sbjct: 123 IVLKQEDGEIDSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDLETPVLLQEFV 182

Query: 181 NHGGVMFKVYVIGEESVCVKRKSLPDVAETEEELEEKAEGAMKFSQISRAEKQDKHGGGG 240
           NHGGVMFKVYV GEESVCVKRKSLPDV ETEEELE+K +GAMKFSQISRAE++ +   G 
Sbjct: 183 NHGGVMFKVYVAGEESVCVKRKSLPDVTETEEELEKKPDGAMKFSQISRAEEKSEKCDGA 242

Query: 241 G--GGGEKEEEIEMPSSKLVAEVSRGLREAMGLRLFNFDMIRDRNGNYFVIDINYLPGFA 300
                 EKEEEIEMP  K+V EVS GL+EAMG+RLFNFDMIRD NG Y+VIDINYLPGFA
Sbjct: 243 EKKAAAEKEEEIEMPPEKIVREVSNGLKEAMGIRLFNFDMIRDGNGTYYVIDINYLPGFA 302

Query: 301 VLPNYEPFLTKFLKEVAERKKKKAEADAIGDAEPAEPKPRCCCF 339
           VLP YE FLTKF KEVAE+KKKK E D     E  + KPRCCCF
Sbjct: 303 VLPEYEQFLTKFFKEVAEKKKKKVEEDDSA-IEDGKAKPRCCCF 345

BLAST of MC02g1017 vs. ExPASy TrEMBL
Match: A0A6J1FTQ5 (Inositol-tetrakisphosphate 1-kinase OS=Cucurbita moschata OX=3662 GN=LOC111448363 PE=3 SV=1)

HSP 1 Score: 507 bits (1306), Expect = 1.25e-178
Identity = 261/344 (75.87%), Postives = 295/344 (85.76%), Query Frame = 0

Query: 1   AAMASSSERLRIGYAFPPNKEHNVIRPSLIDYAKLHGVDLVRIDLQTPLLHQAPFHCIIH 60
           ++ +SSS RLR+GYAFPPNKE NVIRPSL+DYAKLHGVDLVRIDLQTPLLHQ PFHCIIH
Sbjct: 3   SSSSSSSNRLRVGYAFPPNKERNVIRPSLVDYAKLHGVDLVRIDLQTPLLHQGPFHCIIH 62

Query: 61  KLYDDAWAEHLQEFASKNPDVVVLEQPELISRLNDRVSMLDVVKEVKVSESGVKIEVPKQ 120
           KLYDDAWAEHLQ+FA KNPDVVV+++P+LI+RL DRVSMLDVV +VK S+SGV+IEVPKQ
Sbjct: 63  KLYDDAWAEHLQDFALKNPDVVVVDRPDLIARLYDRVSMLDVVSQVKASDSGVRIEVPKQ 122

Query: 121 VILNELE---DPIRDLEVKFPVIAKPLRSDGSAQSHEMSLVFNRRGLKELESPVLLQEFV 180
           ++L + +   D IRDL+VKFPVIAKPL SDGSA+SHEMSLVFNRRGLK+L+ PVLLQEFV
Sbjct: 123 IVLKQEDGEIDSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDLDRPVLLQEFV 182

Query: 181 NHGGVMFKVYVIGEESVCVKRKSLPDVAETEEELEEKAEGAMKFSQISRAEKQDKHGGGG 240
           NHGGVMFKVYV GEESVCVKRKSLPDVAETEEELE+K +GAMKFSQISRAE++ +   G 
Sbjct: 183 NHGGVMFKVYVAGEESVCVKRKSLPDVAETEEELEKKQDGAMKFSQISRAEEKSEKCNGA 242

Query: 241 GGGGEKEE--EIEMPSSKLVAEVSRGLREAMGLRLFNFDMIRDRNGNYFVIDINYLPGFA 300
               EK+E  EIEMP  K+V EVS GLREAMG+RLFNFDMIRD NG Y+VIDINYLPGFA
Sbjct: 243 ----EKKEAEEIEMPPEKIVREVSNGLREAMGIRLFNFDMIRDGNGTYYVIDINYLPGFA 302

Query: 301 VLPNYEPFLTKFLKEVAERKKKKAEADAIGDAEPAEPKPRCCCF 339
           VLP YE FLTKF KEVAE+KKKK   +     E  + KPRCCCF
Sbjct: 303 VLPEYEQFLTKFFKEVAEKKKKKKVEEDDSAIEDGKAKPRCCCF 342

BLAST of MC02g1017 vs. ExPASy TrEMBL
Match: A0A6J1L0P8 (Inositol-tetrakisphosphate 1-kinase OS=Cucurbita maxima OX=3661 GN=LOC111499970 PE=3 SV=1)

HSP 1 Score: 493 bits (1269), Expect = 1.26e-172
Identity = 255/343 (74.34%), Postives = 291/343 (84.84%), Query Frame = 0

Query: 1   AAMASSSERLRIGYAFPPNKEHNVIRPSLIDYAKLHGVDLVRIDLQTPLLHQAPFHCIIH 60
           ++ +SSS+  R+GYAFPPNKE NVIRPSLIDYAKLHGVDLVRIDLQTP+LHQ PFHCIIH
Sbjct: 8   SSSSSSSDGFRVGYAFPPNKERNVIRPSLIDYAKLHGVDLVRIDLQTPILHQGPFHCIIH 67

Query: 61  KLYDDAWAEHLQEFASKNPDVVVLEQPELISRLNDRVSMLDVVKEVKVSESGVKIEVPKQ 120
           K+YDDAW + LQ+FASKNPDVVV+++P+ I RL +RVSMLDVV +VKVS+SGVKIEVPKQ
Sbjct: 68  KMYDDAWVDTLQDFASKNPDVVVVDRPDRIERLYNRVSMLDVVNQVKVSDSGVKIEVPKQ 127

Query: 121 VILNELE---DPIRDLEVKFPVIAKPLRSDGSAQSHEMSLVFNRRGLKELESPVLLQEFV 180
           V+LN  +   D IRDLEVKFPVIAKPL S+GSA+SHEMSLV+NRRG K+LE+PVLLQEFV
Sbjct: 128 VVLNRKDGVIDLIRDLEVKFPVIAKPLESNGSAKSHEMSLVYNRRGFKDLETPVLLQEFV 187

Query: 181 NHGGVMFKVYVIGEESVCVKRKSLPDVAETEEELEEKAEGAMKFSQISRAE-KQDKHGGG 240
           NHGGVMFKVYV GE+SVCVKRKSLPDV ETEEELE+K +GAMKFSQISRAE K +K   G
Sbjct: 188 NHGGVMFKVYVAGEQSVCVKRKSLPDVVETEEELEKKTDGAMKFSQISRAEEKSEKCNCG 247

Query: 241 GGGGGEKEEEIEMPSSKLVAEVSRGLREAMGLRLFNFDMIRDRNGNYFVIDINYLPGFAV 300
           G      EEEIEMP  K+V EVSRGLREAMG+RLFNFDMIRDRNG Y+VIDINYLPGFAV
Sbjct: 248 GKKEAAAEEEIEMPPEKVVTEVSRGLREAMGIRLFNFDMIRDRNGTYYVIDINYLPGFAV 307

Query: 301 LPNYEPFLTKFLKEVAERKKKKAEADAIGDAEPAEPKPRCCCF 339
           LP+YE FLTKF  EV ++K  + E  A+ D    + KPRCCCF
Sbjct: 308 LPDYEAFLTKFFNEVMQKKVVEEENSAMEDE---KAKPRCCCF 347

BLAST of MC02g1017 vs. ExPASy TrEMBL
Match: A0A6J1G5K0 (Inositol-tetrakisphosphate 1-kinase OS=Cucurbita moschata OX=3662 GN=LOC111451086 PE=3 SV=1)

HSP 1 Score: 490 bits (1261), Expect = 2.00e-171
Identity = 254/343 (74.05%), Postives = 290/343 (84.55%), Query Frame = 0

Query: 1   AAMASSSERLRIGYAFPPNKEHNVIRPSLIDYAKLHGVDLVRIDLQTPLLHQAPFHCIIH 60
           ++ +SSS+  R+GYAFPPNKE NVIRPSLIDYAKLHGVDLVRIDLQ+P+LHQ PFHCIIH
Sbjct: 7   SSSSSSSDGFRVGYAFPPNKERNVIRPSLIDYAKLHGVDLVRIDLQSPILHQGPFHCIIH 66

Query: 61  KLYDDAWAEHLQEFASKNPDVVVLEQPELISRLNDRVSMLDVVKEVKVSESGVKIEVPKQ 120
           K+YDDAW E LQ+FA KNPDVVV+++P+ I RL +RVSMLDVV +VKVS+SGVKIEVPKQ
Sbjct: 67  KMYDDAWVEKLQDFALKNPDVVVVDRPDRIERLYNRVSMLDVVSQVKVSDSGVKIEVPKQ 126

Query: 121 VILNELE---DPIRDLEVKFPVIAKPLRSDGSAQSHEMSLVFNRRGLKELESPVLLQEFV 180
           V+LN  +   D IRDLEVKFPVIAKPL S+GSA+SHEMSLV+NRRG K+LE+PVLLQEFV
Sbjct: 127 VVLNRKDGVIDLIRDLEVKFPVIAKPLESNGSAKSHEMSLVYNRRGFKDLETPVLLQEFV 186

Query: 181 NHGGVMFKVYVIGEESVCVKRKSLPDVAETEEELEEKAEGAMKFSQISRAE-KQDKHGGG 240
           NHGGVMFKVYV GE+SVCVKRKSLPDV ETEEELE+K +GAMKFSQISRAE K +K   G
Sbjct: 187 NHGGVMFKVYVAGEQSVCVKRKSLPDVVETEEELEKKTDGAMKFSQISRAEEKSEKCNCG 246

Query: 241 GGGGGEKEEEIEMPSSKLVAEVSRGLREAMGLRLFNFDMIRDRNGNYFVIDINYLPGFAV 300
                  EEEIEMP  K+V EVSRGLREAMG+RLFNFDMIRDRNG Y+VIDINYLPGFAV
Sbjct: 247 RKKEAAAEEEIEMPPEKIVTEVSRGLREAMGIRLFNFDMIRDRNGTYYVIDINYLPGFAV 306

Query: 301 LPNYEPFLTKFLKEVAERKKKKAEADAIGDAEPAEPKPRCCCF 339
           LP+YE FLTKF  EV ++K ++ E  AI   E  + KPRCCCF
Sbjct: 307 LPDYEAFLTKFFNEVMQKKVEEEENSAI---EEEKAKPRCCCF 346

BLAST of MC02g1017 vs. ExPASy TrEMBL
Match: A0A6J1G5I0 (Inositol-tetrakisphosphate 1-kinase OS=Cucurbita moschata OX=3662 GN=LOC111451086 PE=3 SV=1)

HSP 1 Score: 490 bits (1261), Expect = 2.86e-171
Identity = 254/343 (74.05%), Postives = 290/343 (84.55%), Query Frame = 0

Query: 1   AAMASSSERLRIGYAFPPNKEHNVIRPSLIDYAKLHGVDLVRIDLQTPLLHQAPFHCIIH 60
           ++ +SSS+  R+GYAFPPNKE NVIRPSLIDYAKLHGVDLVRIDLQ+P+LHQ PFHCIIH
Sbjct: 17  SSSSSSSDGFRVGYAFPPNKERNVIRPSLIDYAKLHGVDLVRIDLQSPILHQGPFHCIIH 76

Query: 61  KLYDDAWAEHLQEFASKNPDVVVLEQPELISRLNDRVSMLDVVKEVKVSESGVKIEVPKQ 120
           K+YDDAW E LQ+FA KNPDVVV+++P+ I RL +RVSMLDVV +VKVS+SGVKIEVPKQ
Sbjct: 77  KMYDDAWVEKLQDFALKNPDVVVVDRPDRIERLYNRVSMLDVVSQVKVSDSGVKIEVPKQ 136

Query: 121 VILNELE---DPIRDLEVKFPVIAKPLRSDGSAQSHEMSLVFNRRGLKELESPVLLQEFV 180
           V+LN  +   D IRDLEVKFPVIAKPL S+GSA+SHEMSLV+NRRG K+LE+PVLLQEFV
Sbjct: 137 VVLNRKDGVIDLIRDLEVKFPVIAKPLESNGSAKSHEMSLVYNRRGFKDLETPVLLQEFV 196

Query: 181 NHGGVMFKVYVIGEESVCVKRKSLPDVAETEEELEEKAEGAMKFSQISRAE-KQDKHGGG 240
           NHGGVMFKVYV GE+SVCVKRKSLPDV ETEEELE+K +GAMKFSQISRAE K +K   G
Sbjct: 197 NHGGVMFKVYVAGEQSVCVKRKSLPDVVETEEELEKKTDGAMKFSQISRAEEKSEKCNCG 256

Query: 241 GGGGGEKEEEIEMPSSKLVAEVSRGLREAMGLRLFNFDMIRDRNGNYFVIDINYLPGFAV 300
                  EEEIEMP  K+V EVSRGLREAMG+RLFNFDMIRDRNG Y+VIDINYLPGFAV
Sbjct: 257 RKKEAAAEEEIEMPPEKIVTEVSRGLREAMGIRLFNFDMIRDRNGTYYVIDINYLPGFAV 316

Query: 301 LPNYEPFLTKFLKEVAERKKKKAEADAIGDAEPAEPKPRCCCF 339
           LP+YE FLTKF  EV ++K ++ E  AI   E  + KPRCCCF
Sbjct: 317 LPDYEAFLTKFFNEVMQKKVEEEENSAI---EEEKAKPRCCCF 356

BLAST of MC02g1017 vs. TAIR 10
Match: AT5G16760.1 (Inositol 1,3,4-trisphosphate 5/6-kinase family protein )

HSP 1 Score: 266.9 bits (681), Expect = 2.2e-71
Identity = 147/318 (46.23%), Postives = 216/318 (67.92%), Query Frame = 0

Query: 5   SSSERLRIGYAFPPNKEHNVIRPSLIDYAKLHGVDLVRIDLQTPLLHQAPFHCIIHKLYD 64
           S  ER  +GYA    K+H+ I+PSLI++++  G+DLV++D    LL Q    CIIHKLYD
Sbjct: 4   SIQERYLVGYALAAKKQHSFIQPSLIEHSRQRGIDLVKLDPTKSLLEQGKLDCIIHKLYD 63

Query: 65  DAWAEHLQEFASKNPDVVVLEQPELISRLNDRVSMLDVVKEVK--VSESGVKIEVPKQVI 124
             W E+L EF  K P V V++ PE I RL++RVSML+V+ +++  VS+S  +  VP+QV+
Sbjct: 64  VYWKENLHEFREKCPGVPVIDLPEAIERLHNRVSMLEVITQLRFPVSDS-ERFGVPEQVV 123

Query: 125 LNE---LEDPIRDLEVKFPVIAKPLRSDGSAQSHEMSLVFNRRGLKELESPVLLQEFVNH 184
           + +   L       E+KFPVIAKPL +DGSA+SH+M L++++ G+K L++P++LQEFVNH
Sbjct: 124 VMDSSVLSGGGALGELKFPVIAKPLDADGSAKSHKMFLIYDQEGMKILKAPIVLQEFVNH 183

Query: 185 GGVMFKVYVIGEESVCVKRKSLPDVAETEEELEEKAEGAMKFSQISRAEKQDKHGGGGGG 244
           GGV+FKVYV+G+   CVKR+SLPD++   EE    ++G++ FSQIS    Q+       G
Sbjct: 184 GGVIFKVYVVGDHVKCVKRRSLPDIS---EEKIGTSKGSLPFSQISNLTAQEDK-NIEYG 243

Query: 245 GGEKEEEIEMPSSKLVAEVSRGLREAMGLRLFNFDMIRDRN--GNYFVIDINYLPGFAVL 304
                E++EMP    + ++++ +RE+MGL LFNFD+IRD      Y +IDINY PG+A +
Sbjct: 244 EDRSLEKVEMPPLSFLTDLAKAMRESMGLNLFNFDVIRDAKDANRYLIIDINYFPGYAKM 303

Query: 305 PNYEPFLTKFLKEVAERK 316
           P+YEP LT+F  ++  +K
Sbjct: 304 PSYEPVLTEFFWDMVTKK 316

BLAST of MC02g1017 vs. TAIR 10
Match: AT4G33770.1 (Inositol 1,3,4-trisphosphate 5/6-kinase family protein )

HSP 1 Score: 214.9 bits (546), Expect = 9.8e-56
Identity = 119/319 (37.30%), Postives = 189/319 (59.25%), Query Frame = 0

Query: 8   ERLRIGYAFPPNKEHNVIRPSLIDYAKLHGVDLVRIDLQTPLLHQAPFHCIIHKLYDDAW 67
           ++L +GYA    K+ + ++P L   A+  G+  V IDL  PL  Q PF  ++HKL    W
Sbjct: 79  QKLVVGYALTSKKKKSFLQPKLELLARRKGIFFVAIDLNRPLSEQGPFDVVLHKLLGKEW 138

Query: 68  AEHLQEFASKNPDVVVLEQPELISRLNDRVSMLDVVKEVKVSESGVKIEVPKQVILNE-- 127
            E ++++  K+P+V VL+ P  I R+ +R SML  + ++K+S+    + VPKQ+++ +  
Sbjct: 139 EEVIEDYQQKHPEVTVLDPPGSIQRIYNRQSMLQGMADLKLSDCSGSLFVPKQMVVLKDS 198

Query: 128 --LEDPIRDLEVKFPVIAKPLRSDGSAQSHEMSLVFNRRGLKELESPVLLQEFVNHGGVM 187
               D + +  +KFP++AKPL  DG+A+SH++ L ++RR L EL+ P++LQEFVNHGGVM
Sbjct: 199 AASADAVVEAGLKFPLVAKPLWIDGTAKSHQLYLAYDRRSLAELDPPLVLQEFVNHGGVM 258

Query: 188 FKVYVIGEESVCVKRKSLPDVAETEEELEEKAEGAMKFSQISRAEKQDKHGGGGGGGGEK 247
           FKV+V+G+    ++R SLP+V+  E   + K +G  +F ++S A                
Sbjct: 259 FKVFVVGDVIKVMRRFSLPNVSNCE---KAKVDGVFQFPRVSSA------AASADNADLD 318

Query: 248 EEEIEMPSSKLVAEVSRGLREAMGLRLFNFDMIRDRNGN--YFVIDINYLPGFAVLPNYE 307
               E+P    +  + + LR  +GLRLFN DMIR+      ++VIDINY PG+  LP+YE
Sbjct: 319 PRVAELPPKPFLEALVKELRSLLGLRLFNIDMIREHGSKNVFYVIDINYFPGYGKLPDYE 378

Query: 308 PFLTKFLKEVAERKKKKAE 321
                F + +A+ K KK +
Sbjct: 379 QVFVDFFQNLAQVKYKKRQ 388

BLAST of MC02g1017 vs. TAIR 10
Match: AT4G08170.2 (Inositol 1,3,4-trisphosphate 5/6-kinase family protein )

HSP 1 Score: 214.9 bits (546), Expect = 9.8e-56
Identity = 123/319 (38.56%), Postives = 189/319 (59.25%), Query Frame = 0

Query: 12  IGYAFPPNKEHNVIRPSLIDYAKLHGVDLVRIDLQTPLLHQAPFHCIIHKLYDDAWAEHL 71
           +GYA    K  + ++P L   A+  G+  V ID   PL  Q PF  ++HK     W   L
Sbjct: 43  VGYALTSKKIKSFLQPKLEGLARNKGILFVAIDQNKPLSEQGPFDIVLHKQIGKEWRRIL 102

Query: 72  QEFASKNPDVVVLEQPELISRLNDRVSMLDVVKEVKVSESGVKIEVPKQVIL----NELE 131
           +EF   +PDV VL+ P+ I  L +R SML  V ++ +S+S  ++ VPKQ+++    + + 
Sbjct: 103 EEFRLAHPDVTVLDPPDAILHLRNRQSMLQCVADMNLSDSNGRVGVPKQLVIKKDASSIP 162

Query: 132 DPIRDLEVKFPVIAKPLRSDGSAQSHEMSLVFNRRGLKELESPVLLQEFVNHGGVMFKVY 191
           + + +  ++ P++AKPL +DGSA+SHE+SL +++  L +LE P++LQEFVNHGGV+FKVY
Sbjct: 163 EAVNNAGLRLPLVAKPLVADGSAKSHELSLAYDQHSLLKLEPPLVLQEFVNHGGVLFKVY 222

Query: 192 VIGEESVCVKRKSLPDVAETEEELEEKAEGAMKFSQISRAEKQDKHGGGGGGGGEKEEEI 251
           ++GE    V+R SLPDV+  E     K+ G  +F ++S A              + +  I
Sbjct: 223 IVGEAIRVVRRFSLPDVSRRE---LPKSAGVFRFPRVSCA-------AASADDADLDPSI 282

Query: 252 -EMPSSKLVAEVSRGLREAMGLRLFNFDMIRDR--NGNYFVIDINYLPGFAVLPNYEPFL 311
            E+P   L+  +++ LR  +GLRLFN D+IR+      ++VIDINY PG+  +P YE   
Sbjct: 283 AELPPRPLLERLAKELRRGLGLRLFNLDIIREHGTRDRFYVIDINYFPGYGKMPEYEHVF 342

Query: 312 TKFLKEVAERK-KKKAEAD 323
           T FL  V + + KK+A AD
Sbjct: 343 TDFLLSVVQSQCKKRALAD 351

BLAST of MC02g1017 vs. TAIR 10
Match: AT4G33770.2 (Inositol 1,3,4-trisphosphate 5/6-kinase family protein )

HSP 1 Score: 199.9 bits (507), Expect = 3.3e-51
Identity = 111/290 (38.28%), Postives = 173/290 (59.66%), Query Frame = 0

Query: 37  GVDLVRIDLQTPLLHQAPFHCIIHKLYDDAWAEHLQEFASKNPDVVVLEQPELISRLNDR 96
           G+  V IDL  PL  Q PF  ++HKL    W E ++++  K+P+V VL+ P  I R+ +R
Sbjct: 15  GIFFVAIDLNRPLSEQGPFDVVLHKLLGKEWEEVIEDYQQKHPEVTVLDPPGSIQRIYNR 74

Query: 97  VSMLDVVKEVKVSESGVKIEVPKQVILNE----LEDPIRDLEVKFPVIAKPLRSDGSAQS 156
            SML  + ++K+S+    + VPKQ+++ +      D + +  +KFP++AKPL  DG+A+S
Sbjct: 75  QSMLQGMADLKLSDCSGSLFVPKQMVVLKDSAASADAVVEAGLKFPLVAKPLWIDGTAKS 134

Query: 157 HEMSLVFNRRGLKELESPVLLQEFVNHGGVMFKVYVIGEESVCVKRKSLPDVAETEEELE 216
           H++ L ++RR L EL+ P++LQEFVNHGGVMFKV+V+G+    ++R SLP+V+  E   +
Sbjct: 135 HQLYLAYDRRSLAELDPPLVLQEFVNHGGVMFKVFVVGDVIKVMRRFSLPNVSNCE---K 194

Query: 217 EKAEGAMKFSQISRAEKQDKHGGGGGGGGEKEEEIEMPSSKLVAEVSRGLREAMGLRLFN 276
            K +G  +F ++S A                    E+P    +  + + LR  +GLRLFN
Sbjct: 195 AKVDGVFQFPRVSSA------AASADNADLDPRVAELPPKPFLEALVKELRSLLGLRLFN 254

Query: 277 FDMIRDRNGN--YFVIDINYLPGFAVLPNYEPFLTKFLKEVAERKKKKAE 321
            DMIR+      ++VIDINY PG+  LP+YE     F + +A+ K KK +
Sbjct: 255 IDMIREHGSKNVFYVIDINYFPGYGKLPDYEQVFVDFFQNLAQVKYKKRQ 295

BLAST of MC02g1017 vs. TAIR 10
Match: AT4G08170.1 (Inositol 1,3,4-trisphosphate 5/6-kinase family protein )

HSP 1 Score: 181.8 bits (460), Expect = 9.2e-46
Identity = 105/264 (39.77%), Postives = 163/264 (61.74%), Query Frame = 0

Query: 67  WAEHLQEFASKNPDVVVLEQPELISRLNDRVSMLDVVKEVKVSESGVKIEVPKQVIL--- 126
           W   L+EF   +PDV VL+ P+ I  L +R SML  V ++ +S+S  ++ VPKQ+++   
Sbjct: 10  WRRILEEFRLAHPDVTVLDPPDAILHLRNRQSMLQCVADMNLSDSNGRVGVPKQLVIKKD 69

Query: 127 -NELEDPIRDLEVKFPVIAKPLRSDGSAQSHEMSLVFNRRGLKELESPVLLQEFVNHGGV 186
            + + + + +  ++ P++AKPL +DGSA+SHE+SL +++  L +LE P++LQEFVNHGGV
Sbjct: 70  ASSIPEAVNNAGLRLPLVAKPLVADGSAKSHELSLAYDQHSLLKLEPPLVLQEFVNHGGV 129

Query: 187 MFKVYVIGEESVCVKRKSLPDVAETEEELEEKAEGAMKFSQISRAEKQDKHGGGGGGGGE 246
           +FKVY++GE    V+R SLPDV+  E     K+ G  +F ++S A              +
Sbjct: 130 LFKVYIVGEAIRVVRRFSLPDVSRRE---LPKSAGVFRFPRVSCA-------AASADDAD 189

Query: 247 KEEEI-EMPSSKLVAEVSRGLREAMGLRLFNFDMIRDR--NGNYFVIDINYLPGFAVLPN 306
            +  I E+P   L+  +++ LR  +GLRLFN D+IR+      ++VIDINY PG+  +P 
Sbjct: 190 LDPSIAELPPRPLLERLAKELRRGLGLRLFNLDIIREHGTRDRFYVIDINYFPGYGKMPE 249

Query: 307 YEPFLTKFLKEVAERK-KKKAEAD 323
           YE   T FL  V + + KK+A AD
Sbjct: 250 YEHVFTDFLLSVVQSQCKKRALAD 263

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SBA53.1e-7046.23Inositol-tetrakisphosphate 1-kinase 1 OS=Arabidopsis thaliana OX=3702 GN=ITPK1 P... [more]
Q84Y015.1e-6545.19Inositol-tetrakisphosphate 1-kinase 1 OS=Zea mays OX=4577 GN=ITPK1 PE=2 SV=1[more]
A2X4M89.5e-6444.48Inositol-tetrakisphosphate 1-kinase 4 OS=Oryza sativa subsp. indica OX=39946 GN=... [more]
Q6K7B89.5e-6444.48Inositol-tetrakisphosphate 1-kinase 4 OS=Oryza sativa subsp. japonica OX=39947 G... [more]
A0JJZ61.2e-6343.95Inositol-tetrakisphosphate 1-kinase 5 OS=Oryza sativa subsp. indica OX=39946 GN=... [more]
Match NameE-valueIdentityDescription
XP_022986561.17.43e-18176.45inositol-tetrakisphosphate 1-kinase 1-like [Cucurbita maxima][more]
XP_023512946.18.28e-18176.16inositol-tetrakisphosphate 1-kinase 1-like [Cucurbita pepo subsp. pepo][more]
KAG6571172.19.34e-17976.16Inositol-tetrakisphosphate 1-kinase 1, partial [Cucurbita argyrosperma subsp. so... [more]
XP_022943687.12.58e-17875.87inositol-tetrakisphosphate 1-kinase 1-like [Cucurbita moschata][more]
XP_023007496.12.60e-17274.34inositol-tetrakisphosphate 1-kinase 1-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1JBH83.60e-18176.45Inositol-tetrakisphosphate 1-kinase OS=Cucurbita maxima OX=3661 GN=LOC111484264 ... [more]
A0A6J1FTQ51.25e-17875.87Inositol-tetrakisphosphate 1-kinase OS=Cucurbita moschata OX=3662 GN=LOC11144836... [more]
A0A6J1L0P81.26e-17274.34Inositol-tetrakisphosphate 1-kinase OS=Cucurbita maxima OX=3661 GN=LOC111499970 ... [more]
A0A6J1G5K02.00e-17174.05Inositol-tetrakisphosphate 1-kinase OS=Cucurbita moschata OX=3662 GN=LOC11145108... [more]
A0A6J1G5I02.86e-17174.05Inositol-tetrakisphosphate 1-kinase OS=Cucurbita moschata OX=3662 GN=LOC11145108... [more]
Match NameE-valueIdentityDescription
AT5G16760.12.2e-7146.23Inositol 1,3,4-trisphosphate 5/6-kinase family protein [more]
AT4G33770.19.8e-5637.30Inositol 1,3,4-trisphosphate 5/6-kinase family protein [more]
AT4G08170.29.8e-5638.56Inositol 1,3,4-trisphosphate 5/6-kinase family protein [more]
AT4G33770.23.3e-5138.28Inositol 1,3,4-trisphosphate 5/6-kinase family protein [more]
AT4G08170.19.2e-4639.77Inositol 1,3,4-trisphosphate 5/6-kinase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR041429Inositol-tetrakisphosphate 1-kinase, N-terminalPFAMPF17927Ins134_P3_kin_Ncoord: 11..90
e-value: 5.7E-22
score: 77.9
NoneNo IPR availableGENE3D3.30.470.20coord: 88..293
e-value: 4.2E-57
score: 195.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 213..250
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 212..250
NoneNo IPR availablePANTHERPTHR14217:SF24INOSITOL-TETRAKISPHOSPHATE 1-KINASE 1coord: 3..317
NoneNo IPR availableSUPERFAMILY56059Glutathione synthetase ATP-binding domain-likecoord: 87..304
IPR040464Inositol 1,3,4-trisphosphate 5/6-kinase, ATP-grasp domainPFAMPF05770Ins134_P3_kincoord: 114..309
e-value: 7.3E-65
score: 218.2
IPR008656Inositol-tetrakisphosphate 1-kinasePIRSFPIRSF038186ITPKcoord: 2..323
e-value: 5.3E-104
score: 345.7
IPR008656Inositol-tetrakisphosphate 1-kinasePANTHERPTHR14217INOSITOL-TETRAKISPHOSPHATE 1-KINASEcoord: 3..317
IPR011761ATP-grasp foldPROSITEPS50975ATP_GRASPcoord: 107..320
score: 16.535807

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC02g1017.1MC02g1017.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0052746 inositol phosphorylation
biological_process GO:0032957 inositol trisphosphate metabolic process
molecular_function GO:0005524 ATP binding
molecular_function GO:0052726 inositol-1,3,4-trisphosphate 5-kinase activity
molecular_function GO:0052725 inositol-1,3,4-trisphosphate 6-kinase activity
molecular_function GO:0047325 inositol tetrakisphosphate 1-kinase activity
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0046872 metal ion binding