MC02g1002 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC02g1002
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	alpha/beta-Hydrolases superfamily protein
Location	MC02: 8030893 .. 8031830 (+)
RNA-Seq Expression	MC02g1002
Synteny	MC02g1002

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTTCCATTATAAGTCTCTACACGGAGCTTCATTTCTGCTCATCAATGGCGTCTTCCCCCAAAAATCTCGGCATCGCTCACGATTTCTTCCCCTTCTTCAGAGTCTTCAGCGACGGCCGCGTCCATCGACTAATTACCCCCAACGTTGTCCCCGCCTCCGACGACCCCCAATCCACTTTCCGATCCAAAGACGTCACCATCTCGCCCCAACCCCACATCACCGCCAGAATTTTCATCCCCTCCTCCGCGGCCGACGACGACGCGTGCCGGCGCCTCCCCCTCCTCTTCTACGTCCACGGCGGCGCCTTCTGCATCGAATCCGCCTTCAGTTCCCAATACCACACCCACGTCGGCTCCCTCGCCGCCCGGGCCCACGCCGTGGCCGTCTCCGTCGAGTACCGCCTCGCGCCGGAGCACCCGATCCCGGCCTGCTACGACGATTGCTGGGCGGCGCTGAAATGGGTCGCGGCGCACGCGAACGGAGACGGATCGGAGCCCTGGCTGAACAATTTCGCCGATTTTGGGCGGATCTGTTTGGCCGGCGATTCGGCCGGCGCGAATATCTGCCATTATCTGGCTTCCCGGGCGGGATCGGAGGATTTGGGGGAATGGCGTTGATTCATCCGTTTTTTGGGGATGGGGGAGATAACAGGCTGTGGAAGTATTTGTGTCCGGGGATTAGTGGGGAAGACGATCCTAGCCTGAGACCGGCCGCAGAGGATCTGCGGAAACTGGCCTGCCGGAGAGTGGCGGTGTTTTTGGCGGAGGAGGATTTTTTGAAGGAGGGAGGGAGGAAGTACGATGGGGAATTGAGGAGGAGCGGGTGGAGAGGGGCGGCGGAGACGGTGGAGCACGGCGGAGAGGGGCATGTGTTTCATTTGATGAAGCCGGAGTATGAGAAGGCTGATGATTTGATGCAAAAATTAGCCTCTTTCGTT

mRNA sequence

TTTTCCATTATAAGTCTCTACACGGAGCTTCATTTCTGCTCATCAATGGCGTCTTCCCCCAAAAATCTCGGCATCGCTCACGATTTCTTCCCCTTCTTCAGAGTCTTCAGCGACGGCCGCGTCCATCGACTAATTACCCCCAACGTTGTCCCCGCCTCCGACGACCCCCAATCCACTTTCCGATCCAAAGACGTCACCATCTCGCCCCAACCCCACATCACCGCCAGAATTTTCATCCCCTCCTCCGCGGCCGACGACGACGCGTGCCGGCGCCTCCCCCTCCTCTTCTACGTCCACGGCGGCGCCTTCTGCATCGAATCCGCCTTCAGTTCCCAATACCACACCCACGTCGGCTCCCTCGCCGCCCGGGCCCACGCCGTGGCCGTCTCCGTCGAGTACCGCCTCGCGCCGGAGCACCCGATCCCGGCCTGCTACGACGATTGCTGGGCGGCGCTGAAATGGGTCGCGGCGCACGCGAACGGAGACGGATCGGAGCCCTGGCTGAACAATTTCGCCGATTTTGGGCGGATCTGTTTGGCCGGCGATTCGGCCGGCGCGAATATCTGCCATTATCTGGCTTCCCGGGCGGGATCGGAGGATTTGGGAATGGCGTTGATTCATCCGTTTTTTGGGGATGGGGGAGATAACAGGCTGTGGAAGTATTTGTGTCCGGGGATTAGTGGGGAAGACGATCCTAGCCTGAGACCGGCCGCAGAGGATCTGCGGAAACTGGCCTGCCGGAGAGTGGCGGTGTTTTTGGCGGAGGAGGATTTTTTGAAGGAGGGAGGGAGGAAGTACGATGGGGAATTGAGGAGGAGCGGGTGGAGAGGGGCGGCGGAGACGGTGGAGCACGGCGGAGAGGGGCATGTGTTTCATTTGATGAAGCCGGAGTATGAGAAGGCTGATGATTTGATGCAAAAATTAGCCTCTTTCGTT

Coding sequence (CDS)

TTTTCCATTATAAGTCTCTACACGGAGCTTCATTTCTGCTCATCAATGGCGTCTTCCCCCAAAAATCTCGGCATCGCTCACGATTTCTTCCCCTTCTTCAGAGTCTTCAGCGACGGCCGCGTCCATCGACTAATTACCCCCAACGTTGTCCCCGCCTCCGACGACCCCCAATCCACTTTCCGATCCAAAGACGTCACCATCTCGCCCCAACCCCACATCACCGCCAGAATTTTCATCCCCTCCTCCGCGGCCGACGACGACGCGTGCCGGCGCCTCCCCCTCCTCTTCTACGTCCACGGCGGCGCCTTCTGCATCGAATCCGCCTTCAGTTCCCAATACCACACCCACGTCGGCTCCCTCGCCGCCCGGGCCCACGCCGTGGCCGTCTCCGTCGAGTACCGCCTCGCGCCGGAGCACCCGATCCCGGCCTGCTACGACGATTGCTGGGCGGCGCTGAAATGGGTCGCGGCGCACGCGAACGGAGACGGATCGGAGCCCTGGCTGAACAATTTCGCCGATTTTGGGCGGATCTGTTTGGCCGGCGATTCGGCCGGCGCGAATATCTGCCATTATCTGGCTTCCCGGGCGGGATCGGAGGATTTGGGAATGGCGTTGATTCATCCGTTTTTTGGGGATGGGGGAGATAACAGGCTGTGGAAGTATTTGTGTCCGGGGATTAGTGGGGAAGACGATCCTAGCCTGAGACCGGCCGCAGAGGATCTGCGGAAACTGGCCTGCCGGAGAGTGGCGGTGTTTTTGGCGGAGGAGGATTTTTTGAAGGAGGGAGGGAGGAAGTACGATGGGGAATTGAGGAGGAGCGGGTGGAGAGGGGCGGCGGAGACGGTGGAGCACGGCGGAGAGGGGCATGTGTTTCATTTGATGAAGCCGGAGTATGAGAAGGCTGATGATTTGATGCAAAAATTAGCCTCTTTCGTT

Protein sequence

FSIISLYTELHFCSSMASSPKNLGIAHDFFPFFRVFSDGRVHRLITPNVVPASDDPQSTFRSKDVTISPQPHITARIFIPSSAADDDACRRLPLLFYVHGGAFCIESAFSSQYHTHVGSLAARAHAVAVSVEYRLAPEHPIPACYDDCWAALKWVAAHANGDGSEPWLNNFADFGRICLAGDSAGANICHYLASRAGSEDLGMALIHPFFGDGGDNRLWKYLCPGISGEDDPSLRPAAEDLRKLACRRVAVFLAEEDFLKEGGRKYDGELRRSGWRGAAETVEHGGEGHVFHLMKPEYEKADDLMQKLASFV

Homology

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BLAST of MC02g1002 vs. ExPASy Swiss-Prot
Match: Q9LMA7 (Probable carboxylesterase 1 OS=Arabidopsis thaliana OX=3702 GN=CXE1 PE=2 SV=1)

HSP 1 Score: 246.5 bits (628), Expect = 3.9e-64
Identity = 127/309 (41.10%), Postives = 174/309 (56.31%), Query Frame = 0


Query: 25  IAHDFFPFFRVFSDGRVHRLITPNVVPASDDPQSTFRSKDVTISPQPHITARIFIPSSAA 84
           IA D+ P FR+F +G + RL+    VP S +P++   SKD   SP+ +++ RI++P ++ 
Sbjct: 5   IAFDYSPRFRIFKNGGIERLVPETFVPPSLNPENGVVSKDAVYSPEKNLSLRIYLPQNSV 64

Query: 85  DDDACRRLPLLFYVHGGAFCIESAFSSQYHTHVGSLAARAHAVAVSVEYRLAPEHPIPAC 144
            +   +++PLL Y HGG F +E+AFS  YHT + S  +    +AVSVEYR APEHPIP  
Sbjct: 65  YETGEKKIPLLVYFHGGGFIMETAFSPIYHTFLTSAVSATDCIAVSVEYRRAPEHPIPTL 124

Query: 145 YDDCWAALKWVAAHANGDGSEPWLNNFADFGRICLAGDSAGANICHYLASRAGSEDL--- 204
           Y+D W A++W+  H    G E WLN  ADF ++ LAGDSAGANI H++A R   E L   
Sbjct: 125 YEDSWDAIQWIFTHITRSGPEDWLNKHADFSKVFLAGDSAGANIAHHMAIRVDKEKLPPE 184

Query: 205 -----GMALIHPFFGDGG------------DNRLWKYLCPGI-SGEDDPSLRPAAEDLRK 264
                GM L HP+F                  RLW+   P   +G +DP +     DL  
Sbjct: 185 NFKISGMILFHPYFLSKALIEEMEVEAMRYYERLWRIASPDSGNGVEDPWINVVGSDLTG 244

Query: 265 LACRRVAVFLAEEDFLKEGGRKYDGELRRSGWRGAAETVEHGGEGHVFHLMKPEYEKADD 313
           L CRRV V +A  D L  GG  Y  EL +SGW G  + +E   EGHVFHL  P+ E A  
Sbjct: 245 LGCRRVLVMVAGNDVLARGGWSYVAELEKSGWIGKVKVMETKEEGHVFHLRDPDSENARR 304

[Close]

BLAST of MC02g1002 vs. ExPASy Swiss-Prot
Match: Q9FX94 (Probable carboxylesterase 5 OS=Arabidopsis thaliana OX=3702 GN=CXE5 PE=2 SV=1)

HSP 1 Score: 245.4 bits (625), Expect = 8.8e-64
Identity = 127/313 (40.58%), Postives = 183/313 (58.47%), Query Frame = 0


Query: 25  IAHDFFPFFRVFSDGRVHRLITPNVVPASDDPQSTFRSKDVTISPQPHITARIFIPSSAA 84
           IA +F PF R++ DGRV RLI  + +PAS DP     SKDV  SP+ +++ R+F+P  + 
Sbjct: 5   IASEFLPFCRIYKDGRVERLIGTDTIPASLDPTYDVVSKDVIYSPENNLSVRLFLPHKST 64

Query: 85  DDDACRRLPLLFYVHGGAFCIESAFSSQYHTHVGSLAARAHAVAVSVEYRLAPEHPIPAC 144
              A  +LPLL Y+HGGA+ IES FS  YH ++  +   A+ +AVSV+YR APE P+PA 
Sbjct: 65  KLTAGNKLPLLIYIHGGAWIIESPFSPLYHNYLTEVVKSANCLAVSVQYRRAPEDPVPAA 124

Query: 145 YDDCWAALKWVAAHANGDGSEPWLNNFADFGRICLAGDSAGANICHYLASRAGSE---DL 204
           Y+D W+A++W+ AH+NG G   W+N  ADFG++ L GDSAG NI H++A +AG E   DL
Sbjct: 125 YEDVWSAIQWIFAHSNGSGPVDWINKHADFGKVFLGGDSAGGNISHHMAMKAGKEKKLDL 184

Query: 205 ---GMALIHP-FFGD--------------GGDNRLWKYLCP--GISGEDDP--SLRPAAE 264
              G+A++HP F+G                G   +W+ +     ++G DDP  ++  +  
Sbjct: 185 KIKGIAVVHPAFWGTDPVDEYDVQDKETRSGIAEIWEKIASPNSVNGTDDPLFNVNGSGS 244

Query: 265 DLRKLACRRVAVFLAEEDFLKEGGRKYDGELRRSGWRGAAETVEHGGEGHVFHLMKPEYE 313
           D   L C +V V +A +D     G  Y  +L +  W G  E VE  GE HVFHL  P+ +
Sbjct: 245 DFSGLGCDKVLVAVAGKDVFVRQGLAYAAKLEKCEWEGTVEVVEEEGEDHVFHLQNPKSD 304

[Close]

BLAST of MC02g1002 vs. ExPASy Swiss-Prot
Match: Q9SMN0 (Probable carboxylesterase 12 OS=Arabidopsis thaliana OX=3702 GN=CXE12 PE=1 SV=1)

HSP 1 Score: 245.0 bits (624), Expect = 1.1e-63
Identity = 136/317 (42.90%), Postives = 179/317 (56.47%), Query Frame = 0


Query: 25  IAHDFFPFFRVFSDGRVHRLITPNVVPASDDPQSTFRSKDVTISPQPHITARIFIPSSAA 84
           IA D  P  +++  GR+ RL+    VP S +PQ+   SKDV  S   +++ RI++P  AA
Sbjct: 5   IAVDCSPLLKIYKSGRIERLMGEATVPPSSEPQNGVVSKDVVYSADNNLSVRIYLPEKAA 64

Query: 85  DDDACRRLPLLFYVHGGAFCIESAFSSQYHTHVGSLAARAHAVAVSVEYRLAPEHPIPAC 144
             +   +LPLL Y HGG F IE+AFS  YHT + +  + ++ VAVSV+YR APEHPI   
Sbjct: 65  -AETDSKLPLLVYFHGGGFIIETAFSPTYHTFLTTSVSASNCVAVSVDYRRAPEHPISVP 124

Query: 145 YDDCWAALKWVAAHANGDGSEPWLNNFADFGRICLAGDSAGANICHYLASRAGSEDL--- 204
           +DD W ALKWV  H  G G E WLN  ADF R+ L+GDSAGANI H++A RA  E L   
Sbjct: 125 FDDSWTALKWVFTHITGSGQEDWLNKHADFSRVFLSGDSAGANIVHHMAMRAAKEKLSPG 184

Query: 205 -------GMALIHPFF--------GDGGDNRL-------WKYLCPGI-SGEDDPSL---R 264
                  G+ L+HP+F         D  D  L       W    P    G DDP L   +
Sbjct: 185 LNDTGISGIILLHPYFWSKTPIDEKDTKDETLRMKIEAFWMMASPNSKDGTDDPLLNVVQ 244

Query: 265 PAAEDLRKLACRRVAVFLAEEDFLKEGGRKYDGELRRSGWRGAAETVEHGGEGHVFHLMK 313
             + DL  L C +V V +AE+D L   G  Y  +L +SGW+G  E VE  GE HVFHL+K
Sbjct: 245 SESVDLSGLGCGKVLVMVAEKDALVRQGWGYAAKLEKSGWKGEVEVVESEGEDHVFHLLK 304

[Close]

BLAST of MC02g1002 vs. ExPASy Swiss-Prot
Match: Q9SMM9 (Probable carboxylesterase 13 OS=Arabidopsis thaliana OX=3702 GN=CXE13 PE=1 SV=1)

HSP 1 Score: 239.6 bits (610), Expect = 4.8e-62
Identity = 132/321 (41.12%), Postives = 178/321 (55.45%), Query Frame = 0


Query: 25  IAHDFFPFFRVFSDGRVHRLITPNVVPASDDPQSTFRSKDVTISPQPHITARIFIPSSA- 84
           IA D+ P   ++  GR+ RL+    VP S +PQ+   SKDV  SP  +++ RI++P  A 
Sbjct: 5   IAADYSPMLIIYKSGRIERLVGETTVPPSSNPQNGVVSKDVVYSPDNNLSLRIYLPEKAA 64

Query: 85  -ADDDACRRLPLLFYVHGGAFCIESAFSSQYHTHVGSLAARAHAVAVSVEYRLAPEHPIP 144
            A+ +A  +LPLL Y HGG F +E+AFS  YHT + +  + +  VAVSV+YR APEHPIP
Sbjct: 65  TAETEASVKLPLLVYFHGGGFLVETAFSPTYHTFLTAAVSASDCVAVSVDYRRAPEHPIP 124

Query: 145 ACYDDCWAALKWVAAHANGDGSEPWLNNFADFGRICLAGDSAGANICHYLASRAGSEDL- 204
             YDD W ALKWV +H  G GSE WLN  ADF ++ LAGDSAGANI H++  +A  + L 
Sbjct: 125 TSYDDSWTALKWVFSHIAGSGSEDWLNKHADFSKVFLAGDSAGANITHHMTMKAAKDKLS 184

Query: 205 ----------GMALIHPFFGDGGD---------------NRLWKYLCPGI-SGEDDPSL- 264
                     G+ L+HP+F                      +W    P    G DDP + 
Sbjct: 185 PESLNESGISGIILVHPYFWSKTPVDDKETTDVAIRTWIESVWTLASPNSKDGSDDPFIN 244

Query: 265 --RPAAEDLRKLACRRVAVFLAEEDFLKEGGRKYDGELRRSGWRG-AAETVEHGGEGHVF 313
             +  + DL  L C +V V +AE+D L   G  Y  +L +S W G   + VE  GEGHVF
Sbjct: 245 VVQSESVDLSGLGCGKVLVMVAEKDALVRQGWGYWEKLGKSRWNGEVLDVVETKGEGHVF 304

[Close]

BLAST of MC02g1002 vs. ExPASy Swiss-Prot
Match: Q9ZQ91 (Probable carboxylesterase 7 OS=Arabidopsis thaliana OX=3702 GN=CXE7 PE=2 SV=1)

HSP 1 Score: 236.1 bits (601), Expect = 5.3e-61
Identity = 127/310 (40.97%), Postives = 167/310 (53.87%), Query Frame = 0


Query: 25  IAHDFFPFFRVFSDGRVHRLITPNVVPASDDPQSTFRSKDVTISPQPHITARIFIPSSAA 84
           IA D  P FRV+  GR+ RL+    VP S  PQ+   SKD+  SP+ +++ RI++P    
Sbjct: 5   IAFDRSPMFRVYKSGRIERLLGETTVPPSLTPQNGVVSKDIIHSPEKNLSLRIYLPEKV- 64

Query: 85  DDDACRRLPLLFYVHGGAFCIESAFSSQYHTHVGSLAARAHAVAVSVEYRLAPEHPIPAC 144
                ++LP+L Y HGG F IE+AFS  YHT + S  A A+ +A+SV YR APE P+P  
Sbjct: 65  ---TVKKLPILIYFHGGGFIIETAFSPPYHTFLTSAVAAANCLAISVNYRRAPEFPVPIP 124

Query: 145 YDDCWAALKWVAAHANGDGSEPWLNNFADFGRICLAGDSAGANICHYLASRAGSEDL--- 204
           Y+D W +LKWV  H  G G E W+N   DFG++ LAGDSAG NI H+L  RA  E L   
Sbjct: 125 YEDSWDSLKWVLTHITGTGPETWINKHGDFGKVFLAGDSAGGNISHHLTMRAKKEKLCDS 184

Query: 205 ---GMALIHPFFGD---------------GGDNRLWKYLCPGI-SGEDDPSLRPAAEDLR 264
              G+ LIHP+F                  G    W+   P    G DDP L     D  
Sbjct: 185 LISGIILIHPYFWSKTPIDEFEVRDVGKTKGVEGSWRVASPNSKQGVDDPWLNVVGSDPS 244

Query: 265 KLACRRVAVFLAEEDFLKEGGRKYDGELRRSGWRGAAETVEHGGEGHVFHLMKPEYEKAD 313
            L C RV V +A +D     G  Y  +L++SGW G  E +E   EGHVFHL  P  + A 
Sbjct: 245 GLGCGRVLVMVAGDDLFVRQGWCYAEKLKKSGWEGEVEVMETKNEGHVFHLKNPNSDNAR 304

[Close]

BLAST of MC02g1002 vs. NCBI nr
Match: XP_004145358.2 (2-hydroxyisoflavanone dehydratase [Cucumis sativus] >KGN61726.1 hypothetical protein Csa_006817 [Cucumis sativus])

HSP 1 Score: 422 bits (1085), Expect = 9.11e-146
Identity = 213/308 (69.16%), Postives = 244/308 (79.22%), Query Frame = 0


Query: 16  MASSP-KNLGIAHDFFPFFRVFSDGRVHRLITPN-VVPA-SDDPQSTFRSKDVTISPQPH 75
           MASS  KN  +A DFFPF RV++DGRV RL+T + +VPA +DDP+S FRSKDVTIS  P 
Sbjct: 1   MASSTNKNDDVAFDFFPFLRVYTDGRVQRLMTTSDIVPADADDPKSPFRSKDVTISTDPA 60

Query: 76  ITARIFIPSSAADDDACRRLPLLFYVHGGAFCIESAFSSQYHTHVGSLAARAHAVAVSVE 135
           ++AR+FIPSSA   D  ++LPLL YVHGGAFCIESAFS QYH HVGSLAA+A+AVAVSVE
Sbjct: 61  VSARVFIPSSA---DPNQKLPLLLYVHGGAFCIESAFSLQYHQHVGSLAAKANAVAVSVE 120

Query: 136 YRLAPEHPIPACYDDCWAALKWVAAHANGDGSEPWLNNFADFGRICLAGDSAGANICHYL 195
           YRLAPEHPIPACY+DCW AL+WVAAH N DGSEPWLN + DF RICLAGDSAGANICHYL
Sbjct: 121 YRLAPEHPIPACYEDCWDALRWVAAHVNRDGSEPWLNTYVDFNRICLAGDSAGANICHYL 180

Query: 196 ASRAGS--EDLG------MALIHPFFGDGGDNRLWKYLCPGISGEDDPSLRPAAEDLRKL 255
           A+RA S  E+LG      MALIHPFFGDGG+NRLWKYLC      +   LRP  EDL KL
Sbjct: 181 AARASSSAEELGGAKVVAMALIHPFFGDGGENRLWKYLC-----SETKLLRPTIEDLAKL 240

Query: 256 ACRRVAVFLAEEDFLKEGGRKYDGELRRSGWRGAAETVEHGGEGHVFHLMKPEYEKADDL 312
            C+RV +FLAE DFLK GG+ Y+ +L+ SGW G  ETVEHG E HVFHL KPE EKA DL
Sbjct: 241 GCKRVKIFLAENDFLKSGGKNYEEDLKSSGWNGTVETVEHGEENHVFHLKKPECEKAVDL 300

[Close]

BLAST of MC02g1002 vs. NCBI nr
Match: XP_008459211.1 (PREDICTED: 2-hydroxyisoflavanone dehydratase-like [Cucumis melo] >KAA0032058.1 2-hydroxyisoflavanone dehydratase-like [Cucumis melo var. makuwa] >TYK13561.1 2-hydroxyisoflavanone dehydratase-like [Cucumis melo var. makuwa])

HSP 1 Score: 411 bits (1056), Expect = 2.34e-141
Identity = 209/308 (67.86%), Postives = 239/308 (77.60%), Query Frame = 0


Query: 16  MASSP-KNLGIAHDFFPFFRVFSDGRVHRL-ITPNVVPA-SDDPQSTFRSKDVTISPQPH 75
           MASS  KN  +A D FPF RV+SDGRV RL IT + VPA +DDP+S FRSKDVTIS  P 
Sbjct: 1   MASSTNKNNEVAFDLFPFLRVYSDGRVQRLMITSDFVPADADDPKSPFRSKDVTISTDPA 60

Query: 76  ITARIFIPSSAADDDACRRLPLLFYVHGGAFCIESAFSSQYHTHVGSLAARAHAVAVSVE 135
           ++AR+FIPSSA   D  ++LPLL YVHGGAFCIESAF+ QYH HVG+LAA A+AVAVSVE
Sbjct: 61  VSARVFIPSSA---DPNQKLPLLLYVHGGAFCIESAFNLQYHQHVGALAAEANAVAVSVE 120

Query: 136 YRLAPEHPIPACYDDCWAALKWVAAHANGDGSEPWLNNFADFGRICLAGDSAGANICHYL 195
           YRLAPEHPIPACYDDCW AL+WVAAH N DG EPWLN + DF RICLAGDSAGANICHYL
Sbjct: 121 YRLAPEHPIPACYDDCWDALRWVAAHVNRDGLEPWLNTYVDFNRICLAGDSAGANICHYL 180

Query: 196 ASRAGS--EDLG------MALIHPFFGDGGDNRLWKYLCPGISGEDDPSLRPAAEDLRKL 255
           A+RA S  E+LG      MALIHPFFGDG ++RLWKYLC      +   +RP  E+L K+
Sbjct: 181 AARASSSSEELGGAKVVAMALIHPFFGDGREDRLWKYLC-----SETRLVRPTKEELAKM 240

Query: 256 ACRRVAVFLAEEDFLKEGGRKYDGELRRSGWRGAAETVEHGGEGHVFHLMKPEYEKADDL 312
            C+RV +FLAE DFLK GGR YD +L+RSGW G  ETVEHGGE HVFHL  PE E A DL
Sbjct: 241 GCKRVKIFLAENDFLKFGGRNYDEDLKRSGWNGVVETVEHGGENHVFHLKNPESENAVDL 300

[Close]

BLAST of MC02g1002 vs. NCBI nr
Match: XP_022986577.1 (probable carboxylesterase 12 [Cucurbita maxima] >XP_022986578.1 probable carboxylesterase 12 [Cucurbita maxima])

HSP 1 Score: 393 bits (1010), Expect = 2.08e-134
Identity = 199/307 (64.82%), Postives = 235/307 (76.55%), Query Frame = 0


Query: 16  MASSPKNLGIAHDFFPFFRVFSDGRVHRL-ITPNVVPA-SDDPQSTFRSKDVTISPQPHI 75
           MASS  +  +A +FFPF R++ +GR+ RL +T ++VPA +DDP S FR+KDVTIS    +
Sbjct: 1   MASSTND--VADEFFPFLRIYKNGRIQRLQVTSDIVPACADDPNSAFRAKDVTISVDSAL 60

Query: 76  TARIFIPSSAADDDACRRLPLLFYVHGGAFCIESAFSSQYHTHVGSLAARAHAVAVSVEY 135
           + RIFIPS+    D  ++LPLL YVHGGAFC ESAF+ QYH+HVGSLAA+A+AVAVSVEY
Sbjct: 61  SVRIFIPSAV---DLNQKLPLLLYVHGGAFCFESAFNLQYHSHVGSLAAKANAVAVSVEY 120

Query: 136 RLAPEHPIPACYDDCWAALKWVAAHANGDGSEPWLNNFADFGRICLAGDSAGANICHYLA 195
           RLAPE+PIPACYDDCW AL+W+AAH N DG EPWLN  AD  RICLAGDSAGANICH+LA
Sbjct: 121 RLAPENPIPACYDDCWDALRWIAAHVNRDGPEPWLNEHADLDRICLAGDSAGANICHHLA 180

Query: 196 SRAGS--EDLG------MALIHPFFGDGGDNRLWKYLCPGISGEDDPSLRPAAEDLRKLA 255
            RAGS  E LG      +ALIHPFFGDG ++RLWKYLC      D   LRP A+DL  L 
Sbjct: 181 VRAGSSAEQLGRVKVVALALIHPFFGDGAEDRLWKYLC-----SDTTWLRPTAQDLATLP 240

Query: 256 CRRVAVFLAEEDFLKEGGRKYDGELRRSGWRGAAETVEHGGEGHVFHLMKPEYEKADDLM 312
           CRRV +FLAE+D LK GGR YD +L+ SGW+GA ETVEHGGE HVFHL  PE EKA DLM
Sbjct: 241 CRRVKIFLAEKDSLKFGGRNYDEDLKSSGWKGAVETVEHGGEDHVFHLKMPECEKAVDLM 297

[Close]

BLAST of MC02g1002 vs. NCBI nr
Match: XP_023512082.1 (probable carboxylesterase 12 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 390 bits (1003), Expect = 2.41e-133
Identity = 199/307 (64.82%), Postives = 233/307 (75.90%), Query Frame = 0


Query: 16  MASSPKNLGIAHDFFPFFRVFSDGRVHRL-ITPNVVPA-SDDPQSTFRSKDVTISPQPHI 75
           MASS  +  +A +FFPF R++ DGR+ RL +T ++VPA +DDP+S FR+KDVTIS    +
Sbjct: 1   MASSTND--VADEFFPFLRIYKDGRIQRLQVTSDIVPACADDPKSAFRAKDVTISVDSAL 60

Query: 76  TARIFIPSSAADDDACRRLPLLFYVHGGAFCIESAFSSQYHTHVGSLAARAHAVAVSVEY 135
           + RIFIPS+    D  ++LPLL YVHGGAFC ESAF+ QYH+HVGSLAA+A+AVAVSVEY
Sbjct: 61  SVRIFIPSAV---DLNQKLPLLLYVHGGAFCFESAFNLQYHSHVGSLAAKANAVAVSVEY 120

Query: 136 RLAPEHPIPACYDDCWAALKWVAAHANGDGSEPWLNNFADFGRICLAGDSAGANICHYLA 195
           RLAPE+PIPACYDDCW AL+W+AAH N DG EPWLN  AD  RICLAGDSAGANICH+LA
Sbjct: 121 RLAPENPIPACYDDCWDALRWIAAHVNRDGPEPWLNQHADLDRICLAGDSAGANICHHLA 180

Query: 196 SRAGS--EDLG------MALIHPFFGDGGDNRLWKYLCPGISGEDDPSLRPAAEDLRKLA 255
            RAGS  E LG      +ALIHPFFGDG  +RLWKYLC      D   LRP A+DL  L 
Sbjct: 181 VRAGSSAEQLGRVKVVALALIHPFFGDGAGDRLWKYLC-----SDTTWLRPTAQDLATLP 240

Query: 256 CRRVAVFLAEEDFLKEGGRKYDGELRRSGWRGAAETVEHGGEGHVFHLMKPEYEKADDLM 312
           CRRV +FLAE+D LK GG  YD +L+ SGW GA ETVEHGGE HVFHL  PE EKA DLM
Sbjct: 241 CRRVKIFLAEKDSLKFGGWNYDEDLKSSGWNGAVETVEHGGEDHVFHLKMPECEKAVDLM 297

[Close]

BLAST of MC02g1002 vs. NCBI nr
Match: KAG6571158.1 (putative carboxylesterase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 389 bits (998), Expect = 1.39e-132
Identity = 197/307 (64.17%), Postives = 233/307 (75.90%), Query Frame = 0


Query: 16  MASSPKNLGIAHDFFPFFRVFSDGRVHRL-ITPNVVPA-SDDPQSTFRSKDVTISPQPHI 75
           MASS  +  +A +FFPF R++ DGR+ RL +T ++V A +DDP S FR+KDVTIS    +
Sbjct: 1   MASSTND--VADEFFPFLRIYKDGRIQRLQVTSDIVSACADDPSSAFRAKDVTISVDSAL 60

Query: 76  TARIFIPSSAADDDACRRLPLLFYVHGGAFCIESAFSSQYHTHVGSLAARAHAVAVSVEY 135
           + RIFIPS+    D  ++LPLL YVHGGAFC ESAF+ QYH+HVGSLAA+A+AVAVSVEY
Sbjct: 61  SVRIFIPSAV---DLNQKLPLLLYVHGGAFCFESAFNLQYHSHVGSLAAKANAVAVSVEY 120

Query: 136 RLAPEHPIPACYDDCWAALKWVAAHANGDGSEPWLNNFADFGRICLAGDSAGANICHYLA 195
           RLAPE+PIPACYDDCW AL+W+AAH N DG EPWLN  AD  RICLAGDSAGANICH+LA
Sbjct: 121 RLAPENPIPACYDDCWDALRWIAAHVNRDGPEPWLNQHADLDRICLAGDSAGANICHHLA 180

Query: 196 SRAGS--EDLG------MALIHPFFGDGGDNRLWKYLCPGISGEDDPSLRPAAEDLRKLA 255
            RAG+  E LG      +ALIHPFFGDG  +RLWKYLC      D   LRP A+DL  L 
Sbjct: 181 VRAGTSGEQLGRVKVAALALIHPFFGDGAGDRLWKYLC-----SDTTWLRPTAQDLATLP 240

Query: 256 CRRVAVFLAEEDFLKEGGRKYDGELRRSGWRGAAETVEHGGEGHVFHLMKPEYEKADDLM 312
           CRRV +FLAE+D LK GGR YD +L+ SGW+GA ETVEHGGE HVFHL  PE EKA DLM
Sbjct: 241 CRRVKIFLAEKDSLKFGGRNYDEDLKSSGWKGAVETVEHGGEDHVFHLKMPECEKAVDLM 297

[Close]

BLAST of MC02g1002 vs. ExPASy TrEMBL
Match: A0A0A0LNZ1 (Abhydrolase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G234530 PE=4 SV=1)

HSP 1 Score: 422 bits (1085), Expect = 4.41e-146
Identity = 213/308 (69.16%), Postives = 244/308 (79.22%), Query Frame = 0


Query: 16  MASSP-KNLGIAHDFFPFFRVFSDGRVHRLITPN-VVPA-SDDPQSTFRSKDVTISPQPH 75
           MASS  KN  +A DFFPF RV++DGRV RL+T + +VPA +DDP+S FRSKDVTIS  P 
Sbjct: 1   MASSTNKNDDVAFDFFPFLRVYTDGRVQRLMTTSDIVPADADDPKSPFRSKDVTISTDPA 60

Query: 76  ITARIFIPSSAADDDACRRLPLLFYVHGGAFCIESAFSSQYHTHVGSLAARAHAVAVSVE 135
           ++AR+FIPSSA   D  ++LPLL YVHGGAFCIESAFS QYH HVGSLAA+A+AVAVSVE
Sbjct: 61  VSARVFIPSSA---DPNQKLPLLLYVHGGAFCIESAFSLQYHQHVGSLAAKANAVAVSVE 120

Query: 136 YRLAPEHPIPACYDDCWAALKWVAAHANGDGSEPWLNNFADFGRICLAGDSAGANICHYL 195
           YRLAPEHPIPACY+DCW AL+WVAAH N DGSEPWLN + DF RICLAGDSAGANICHYL
Sbjct: 121 YRLAPEHPIPACYEDCWDALRWVAAHVNRDGSEPWLNTYVDFNRICLAGDSAGANICHYL 180

Query: 196 ASRAGS--EDLG------MALIHPFFGDGGDNRLWKYLCPGISGEDDPSLRPAAEDLRKL 255
           A+RA S  E+LG      MALIHPFFGDGG+NRLWKYLC      +   LRP  EDL KL
Sbjct: 181 AARASSSAEELGGAKVVAMALIHPFFGDGGENRLWKYLC-----SETKLLRPTIEDLAKL 240

Query: 256 ACRRVAVFLAEEDFLKEGGRKYDGELRRSGWRGAAETVEHGGEGHVFHLMKPEYEKADDL 312
            C+RV +FLAE DFLK GG+ Y+ +L+ SGW G  ETVEHG E HVFHL KPE EKA DL
Sbjct: 241 GCKRVKIFLAENDFLKSGGKNYEEDLKSSGWNGTVETVEHGEENHVFHLKKPECEKAVDL 300

[Close]

BLAST of MC02g1002 vs. ExPASy TrEMBL
Match: A0A5D3CP06 (2-hydroxyisoflavanone dehydratase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold299G00380 PE=4 SV=1)

HSP 1 Score: 411 bits (1056), Expect = 1.13e-141
Identity = 209/308 (67.86%), Postives = 239/308 (77.60%), Query Frame = 0


Query: 16  MASSP-KNLGIAHDFFPFFRVFSDGRVHRL-ITPNVVPA-SDDPQSTFRSKDVTISPQPH 75
           MASS  KN  +A D FPF RV+SDGRV RL IT + VPA +DDP+S FRSKDVTIS  P 
Sbjct: 1   MASSTNKNNEVAFDLFPFLRVYSDGRVQRLMITSDFVPADADDPKSPFRSKDVTISTDPA 60

Query: 76  ITARIFIPSSAADDDACRRLPLLFYVHGGAFCIESAFSSQYHTHVGSLAARAHAVAVSVE 135
           ++AR+FIPSSA   D  ++LPLL YVHGGAFCIESAF+ QYH HVG+LAA A+AVAVSVE
Sbjct: 61  VSARVFIPSSA---DPNQKLPLLLYVHGGAFCIESAFNLQYHQHVGALAAEANAVAVSVE 120

Query: 136 YRLAPEHPIPACYDDCWAALKWVAAHANGDGSEPWLNNFADFGRICLAGDSAGANICHYL 195
           YRLAPEHPIPACYDDCW AL+WVAAH N DG EPWLN + DF RICLAGDSAGANICHYL
Sbjct: 121 YRLAPEHPIPACYDDCWDALRWVAAHVNRDGLEPWLNTYVDFNRICLAGDSAGANICHYL 180

Query: 196 ASRAGS--EDLG------MALIHPFFGDGGDNRLWKYLCPGISGEDDPSLRPAAEDLRKL 255
           A+RA S  E+LG      MALIHPFFGDG ++RLWKYLC      +   +RP  E+L K+
Sbjct: 181 AARASSSSEELGGAKVVAMALIHPFFGDGREDRLWKYLC-----SETRLVRPTKEELAKM 240

Query: 256 ACRRVAVFLAEEDFLKEGGRKYDGELRRSGWRGAAETVEHGGEGHVFHLMKPEYEKADDL 312
            C+RV +FLAE DFLK GGR YD +L+RSGW G  ETVEHGGE HVFHL  PE E A DL
Sbjct: 241 GCKRVKIFLAENDFLKFGGRNYDEDLKRSGWNGVVETVEHGGENHVFHLKNPESENAVDL 300

[Close]

BLAST of MC02g1002 vs. ExPASy TrEMBL
Match: A0A1S3C9R7 (2-hydroxyisoflavanone dehydratase-like OS=Cucumis melo OX=3656 GN=LOC103498401 PE=4 SV=1)

HSP 1 Score: 411 bits (1056), Expect = 1.13e-141
Identity = 209/308 (67.86%), Postives = 239/308 (77.60%), Query Frame = 0


Query: 16  MASSP-KNLGIAHDFFPFFRVFSDGRVHRL-ITPNVVPA-SDDPQSTFRSKDVTISPQPH 75
           MASS  KN  +A D FPF RV+SDGRV RL IT + VPA +DDP+S FRSKDVTIS  P 
Sbjct: 1   MASSTNKNNEVAFDLFPFLRVYSDGRVQRLMITSDFVPADADDPKSPFRSKDVTISTDPA 60

Query: 76  ITARIFIPSSAADDDACRRLPLLFYVHGGAFCIESAFSSQYHTHVGSLAARAHAVAVSVE 135
           ++AR+FIPSSA   D  ++LPLL YVHGGAFCIESAF+ QYH HVG+LAA A+AVAVSVE
Sbjct: 61  VSARVFIPSSA---DPNQKLPLLLYVHGGAFCIESAFNLQYHQHVGALAAEANAVAVSVE 120

Query: 136 YRLAPEHPIPACYDDCWAALKWVAAHANGDGSEPWLNNFADFGRICLAGDSAGANICHYL 195
           YRLAPEHPIPACYDDCW AL+WVAAH N DG EPWLN + DF RICLAGDSAGANICHYL
Sbjct: 121 YRLAPEHPIPACYDDCWDALRWVAAHVNRDGLEPWLNTYVDFNRICLAGDSAGANICHYL 180

Query: 196 ASRAGS--EDLG------MALIHPFFGDGGDNRLWKYLCPGISGEDDPSLRPAAEDLRKL 255
           A+RA S  E+LG      MALIHPFFGDG ++RLWKYLC      +   +RP  E+L K+
Sbjct: 181 AARASSSSEELGGAKVVAMALIHPFFGDGREDRLWKYLC-----SETRLVRPTKEELAKM 240

Query: 256 ACRRVAVFLAEEDFLKEGGRKYDGELRRSGWRGAAETVEHGGEGHVFHLMKPEYEKADDL 312
            C+RV +FLAE DFLK GGR YD +L+RSGW G  ETVEHGGE HVFHL  PE E A DL
Sbjct: 241 GCKRVKIFLAENDFLKFGGRNYDEDLKRSGWNGVVETVEHGGENHVFHLKNPESENAVDL 300

[Close]

BLAST of MC02g1002 vs. ExPASy TrEMBL
Match: A0A6J1J7Y5 (probable carboxylesterase 12 OS=Cucurbita maxima OX=3661 GN=LOC111484278 PE=4 SV=1)

HSP 1 Score: 393 bits (1010), Expect = 1.01e-134
Identity = 199/307 (64.82%), Postives = 235/307 (76.55%), Query Frame = 0


Query: 16  MASSPKNLGIAHDFFPFFRVFSDGRVHRL-ITPNVVPA-SDDPQSTFRSKDVTISPQPHI 75
           MASS  +  +A +FFPF R++ +GR+ RL +T ++VPA +DDP S FR+KDVTIS    +
Sbjct: 1   MASSTND--VADEFFPFLRIYKNGRIQRLQVTSDIVPACADDPNSAFRAKDVTISVDSAL 60

Query: 76  TARIFIPSSAADDDACRRLPLLFYVHGGAFCIESAFSSQYHTHVGSLAARAHAVAVSVEY 135
           + RIFIPS+    D  ++LPLL YVHGGAFC ESAF+ QYH+HVGSLAA+A+AVAVSVEY
Sbjct: 61  SVRIFIPSAV---DLNQKLPLLLYVHGGAFCFESAFNLQYHSHVGSLAAKANAVAVSVEY 120

Query: 136 RLAPEHPIPACYDDCWAALKWVAAHANGDGSEPWLNNFADFGRICLAGDSAGANICHYLA 195
           RLAPE+PIPACYDDCW AL+W+AAH N DG EPWLN  AD  RICLAGDSAGANICH+LA
Sbjct: 121 RLAPENPIPACYDDCWDALRWIAAHVNRDGPEPWLNEHADLDRICLAGDSAGANICHHLA 180

Query: 196 SRAGS--EDLG------MALIHPFFGDGGDNRLWKYLCPGISGEDDPSLRPAAEDLRKLA 255
            RAGS  E LG      +ALIHPFFGDG ++RLWKYLC      D   LRP A+DL  L 
Sbjct: 181 VRAGSSAEQLGRVKVVALALIHPFFGDGAEDRLWKYLC-----SDTTWLRPTAQDLATLP 240

Query: 256 CRRVAVFLAEEDFLKEGGRKYDGELRRSGWRGAAETVEHGGEGHVFHLMKPEYEKADDLM 312
           CRRV +FLAE+D LK GGR YD +L+ SGW+GA ETVEHGGE HVFHL  PE EKA DLM
Sbjct: 241 CRRVKIFLAEKDSLKFGGRNYDEDLKSSGWKGAVETVEHGGEDHVFHLKMPECEKAVDLM 297

[Close]

BLAST of MC02g1002 vs. ExPASy TrEMBL
Match: A0A6J1FTL3 (probable carboxylesterase 12 OS=Cucurbita moschata OX=3662 GN=LOC111448682 PE=4 SV=1)

HSP 1 Score: 388 bits (996), Expect = 1.35e-132
Identity = 197/307 (64.17%), Postives = 232/307 (75.57%), Query Frame = 0


Query: 16  MASSPKNLGIAHDFFPFFRVFSDGRVHRL-ITPNVVPA-SDDPQSTFRSKDVTISPQPHI 75
           MASS  +  +A +FFPF R++ DGR+ RL +T ++V A +DDP S FR+KDVTIS    +
Sbjct: 1   MASSTND--VADEFFPFLRIYKDGRIQRLQVTSDIVSACADDPNSAFRAKDVTISVDSAL 60

Query: 76  TARIFIPSSAADDDACRRLPLLFYVHGGAFCIESAFSSQYHTHVGSLAARAHAVAVSVEY 135
           + RIFIPS+    D  ++LPLL YVHGGAFC ESAF+ QYH HVGSLAA+A+AVAVSVEY
Sbjct: 61  SVRIFIPSAV---DLNQKLPLLLYVHGGAFCFESAFNLQYHRHVGSLAAKANAVAVSVEY 120

Query: 136 RLAPEHPIPACYDDCWAALKWVAAHANGDGSEPWLNNFADFGRICLAGDSAGANICHYLA 195
           RLAPE+PIPACYDDCW AL+W+AAH N DG EPWLN  AD  RICLAGDSAGANICH+LA
Sbjct: 121 RLAPENPIPACYDDCWDALRWIAAHVNRDGPEPWLNQHADLDRICLAGDSAGANICHHLA 180

Query: 196 SRAGS--EDLG------MALIHPFFGDGGDNRLWKYLCPGISGEDDPSLRPAAEDLRKLA 255
            RAG+  E LG      +ALIHPFFGDG  +RLWKYLC      D   LRP A+DL  L 
Sbjct: 181 VRAGTSAEQLGRVKVVALALIHPFFGDGAGDRLWKYLC-----SDTTWLRPTAQDLATLP 240

Query: 256 CRRVAVFLAEEDFLKEGGRKYDGELRRSGWRGAAETVEHGGEGHVFHLMKPEYEKADDLM 312
           CRRV +FLAE+D LK GGR YD +L+ SGW+GA ETVEHGGE HVFHL  PE EKA DLM
Sbjct: 241 CRRVKIFLAEKDSLKFGGRNYDEDLKSSGWKGAVETVEHGGEDHVFHLKMPECEKAVDLM 297

[Close]

BLAST of MC02g1002 vs. TAIR 10
Match: AT1G19190.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 246.5 bits (628), Expect = 2.8e-65
Identity = 127/309 (41.10%), Postives = 174/309 (56.31%), Query Frame = 0


Query: 25  IAHDFFPFFRVFSDGRVHRLITPNVVPASDDPQSTFRSKDVTISPQPHITARIFIPSSAA 84
           IA D+ P FR+F +G + RL+    VP S +P++   SKD   SP+ +++ RI++P ++ 
Sbjct: 5   IAFDYSPRFRIFKNGGIERLVPETFVPPSLNPENGVVSKDAVYSPEKNLSLRIYLPQNSV 64

Query: 85  DDDACRRLPLLFYVHGGAFCIESAFSSQYHTHVGSLAARAHAVAVSVEYRLAPEHPIPAC 144
            +   +++PLL Y HGG F +E+AFS  YHT + S  +    +AVSVEYR APEHPIP  
Sbjct: 65  YETGEKKIPLLVYFHGGGFIMETAFSPIYHTFLTSAVSATDCIAVSVEYRRAPEHPIPTL 124

Query: 145 YDDCWAALKWVAAHANGDGSEPWLNNFADFGRICLAGDSAGANICHYLASRAGSEDL--- 204
           Y+D W A++W+  H    G E WLN  ADF ++ LAGDSAGANI H++A R   E L   
Sbjct: 125 YEDSWDAIQWIFTHITRSGPEDWLNKHADFSKVFLAGDSAGANIAHHMAIRVDKEKLPPE 184

Query: 205 -----GMALIHPFFGDGG------------DNRLWKYLCPGI-SGEDDPSLRPAAEDLRK 264
                GM L HP+F                  RLW+   P   +G +DP +     DL  
Sbjct: 185 NFKISGMILFHPYFLSKALIEEMEVEAMRYYERLWRIASPDSGNGVEDPWINVVGSDLTG 244

Query: 265 LACRRVAVFLAEEDFLKEGGRKYDGELRRSGWRGAAETVEHGGEGHVFHLMKPEYEKADD 313
           L CRRV V +A  D L  GG  Y  EL +SGW G  + +E   EGHVFHL  P+ E A  
Sbjct: 245 LGCRRVLVMVAGNDVLARGGWSYVAELEKSGWIGKVKVMETKEEGHVFHLRDPDSENARR 304

[Close]

BLAST of MC02g1002 vs. TAIR 10
Match: AT1G49660.1 (carboxyesterase 5 )

HSP 1 Score: 245.4 bits (625), Expect = 6.2e-65
Identity = 127/313 (40.58%), Postives = 183/313 (58.47%), Query Frame = 0


Query: 25  IAHDFFPFFRVFSDGRVHRLITPNVVPASDDPQSTFRSKDVTISPQPHITARIFIPSSAA 84
           IA +F PF R++ DGRV RLI  + +PAS DP     SKDV  SP+ +++ R+F+P  + 
Sbjct: 5   IASEFLPFCRIYKDGRVERLIGTDTIPASLDPTYDVVSKDVIYSPENNLSVRLFLPHKST 64

Query: 85  DDDACRRLPLLFYVHGGAFCIESAFSSQYHTHVGSLAARAHAVAVSVEYRLAPEHPIPAC 144
              A  +LPLL Y+HGGA+ IES FS  YH ++  +   A+ +AVSV+YR APE P+PA 
Sbjct: 65  KLTAGNKLPLLIYIHGGAWIIESPFSPLYHNYLTEVVKSANCLAVSVQYRRAPEDPVPAA 124

Query: 145 YDDCWAALKWVAAHANGDGSEPWLNNFADFGRICLAGDSAGANICHYLASRAGSE---DL 204
           Y+D W+A++W+ AH+NG G   W+N  ADFG++ L GDSAG NI H++A +AG E   DL
Sbjct: 125 YEDVWSAIQWIFAHSNGSGPVDWINKHADFGKVFLGGDSAGGNISHHMAMKAGKEKKLDL 184

Query: 205 ---GMALIHP-FFGD--------------GGDNRLWKYLCP--GISGEDDP--SLRPAAE 264
              G+A++HP F+G                G   +W+ +     ++G DDP  ++  +  
Sbjct: 185 KIKGIAVVHPAFWGTDPVDEYDVQDKETRSGIAEIWEKIASPNSVNGTDDPLFNVNGSGS 244

Query: 265 DLRKLACRRVAVFLAEEDFLKEGGRKYDGELRRSGWRGAAETVEHGGEGHVFHLMKPEYE 313
           D   L C +V V +A +D     G  Y  +L +  W G  E VE  GE HVFHL  P+ +
Sbjct: 245 DFSGLGCDKVLVAVAGKDVFVRQGLAYAAKLEKCEWEGTVEVVEEEGEDHVFHLQNPKSD 304

[Close]

BLAST of MC02g1002 vs. TAIR 10
Match: AT3G48690.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 245.0 bits (624), Expect = 8.1e-65
Identity = 136/317 (42.90%), Postives = 179/317 (56.47%), Query Frame = 0


Query: 25  IAHDFFPFFRVFSDGRVHRLITPNVVPASDDPQSTFRSKDVTISPQPHITARIFIPSSAA 84
           IA D  P  +++  GR+ RL+    VP S +PQ+   SKDV  S   +++ RI++P  AA
Sbjct: 5   IAVDCSPLLKIYKSGRIERLMGEATVPPSSEPQNGVVSKDVVYSADNNLSVRIYLPEKAA 64

Query: 85  DDDACRRLPLLFYVHGGAFCIESAFSSQYHTHVGSLAARAHAVAVSVEYRLAPEHPIPAC 144
             +   +LPLL Y HGG F IE+AFS  YHT + +  + ++ VAVSV+YR APEHPI   
Sbjct: 65  -AETDSKLPLLVYFHGGGFIIETAFSPTYHTFLTTSVSASNCVAVSVDYRRAPEHPISVP 124

Query: 145 YDDCWAALKWVAAHANGDGSEPWLNNFADFGRICLAGDSAGANICHYLASRAGSEDL--- 204
           +DD W ALKWV  H  G G E WLN  ADF R+ L+GDSAGANI H++A RA  E L   
Sbjct: 125 FDDSWTALKWVFTHITGSGQEDWLNKHADFSRVFLSGDSAGANIVHHMAMRAAKEKLSPG 184

Query: 205 -------GMALIHPFF--------GDGGDNRL-------WKYLCPGI-SGEDDPSL---R 264
                  G+ L+HP+F         D  D  L       W    P    G DDP L   +
Sbjct: 185 LNDTGISGIILLHPYFWSKTPIDEKDTKDETLRMKIEAFWMMASPNSKDGTDDPLLNVVQ 244

Query: 265 PAAEDLRKLACRRVAVFLAEEDFLKEGGRKYDGELRRSGWRGAAETVEHGGEGHVFHLMK 313
             + DL  L C +V V +AE+D L   G  Y  +L +SGW+G  E VE  GE HVFHL+K
Sbjct: 245 SESVDLSGLGCGKVLVMVAEKDALVRQGWGYAAKLEKSGWKGEVEVVESEGEDHVFHLLK 304

[Close]

BLAST of MC02g1002 vs. TAIR 10
Match: AT3G48700.1 (carboxyesterase 13 )

HSP 1 Score: 239.6 bits (610), Expect = 3.4e-63
Identity = 132/321 (41.12%), Postives = 178/321 (55.45%), Query Frame = 0


Query: 25  IAHDFFPFFRVFSDGRVHRLITPNVVPASDDPQSTFRSKDVTISPQPHITARIFIPSSA- 84
           IA D+ P   ++  GR+ RL+    VP S +PQ+   SKDV  SP  +++ RI++P  A 
Sbjct: 5   IAADYSPMLIIYKSGRIERLVGETTVPPSSNPQNGVVSKDVVYSPDNNLSLRIYLPEKAA 64

Query: 85  -ADDDACRRLPLLFYVHGGAFCIESAFSSQYHTHVGSLAARAHAVAVSVEYRLAPEHPIP 144
            A+ +A  +LPLL Y HGG F +E+AFS  YHT + +  + +  VAVSV+YR APEHPIP
Sbjct: 65  TAETEASVKLPLLVYFHGGGFLVETAFSPTYHTFLTAAVSASDCVAVSVDYRRAPEHPIP 124

Query: 145 ACYDDCWAALKWVAAHANGDGSEPWLNNFADFGRICLAGDSAGANICHYLASRAGSEDL- 204
             YDD W ALKWV +H  G GSE WLN  ADF ++ LAGDSAGANI H++  +A  + L 
Sbjct: 125 TSYDDSWTALKWVFSHIAGSGSEDWLNKHADFSKVFLAGDSAGANITHHMTMKAAKDKLS 184

Query: 205 ----------GMALIHPFFGDGGD---------------NRLWKYLCPGI-SGEDDPSL- 264
                     G+ L+HP+F                      +W    P    G DDP + 
Sbjct: 185 PESLNESGISGIILVHPYFWSKTPVDDKETTDVAIRTWIESVWTLASPNSKDGSDDPFIN 244

Query: 265 --RPAAEDLRKLACRRVAVFLAEEDFLKEGGRKYDGELRRSGWRG-AAETVEHGGEGHVF 313
             +  + DL  L C +V V +AE+D L   G  Y  +L +S W G   + VE  GEGHVF
Sbjct: 245 VVQSESVDLSGLGCGKVLVMVAEKDALVRQGWGYWEKLGKSRWNGEVLDVVETKGEGHVF 304

[Close]

BLAST of MC02g1002 vs. TAIR 10
Match: AT2G03550.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 236.1 bits (601), Expect = 3.8e-62
Identity = 127/310 (40.97%), Postives = 167/310 (53.87%), Query Frame = 0


Query: 25  IAHDFFPFFRVFSDGRVHRLITPNVVPASDDPQSTFRSKDVTISPQPHITARIFIPSSAA 84
           IA D  P FRV+  GR+ RL+    VP S  PQ+   SKD+  SP+ +++ RI++P    
Sbjct: 5   IAFDRSPMFRVYKSGRIERLLGETTVPPSLTPQNGVVSKDIIHSPEKNLSLRIYLPEKV- 64

Query: 85  DDDACRRLPLLFYVHGGAFCIESAFSSQYHTHVGSLAARAHAVAVSVEYRLAPEHPIPAC 144
                ++LP+L Y HGG F IE+AFS  YHT + S  A A+ +A+SV YR APE P+P  
Sbjct: 65  ---TVKKLPILIYFHGGGFIIETAFSPPYHTFLTSAVAAANCLAISVNYRRAPEFPVPIP 124

Query: 145 YDDCWAALKWVAAHANGDGSEPWLNNFADFGRICLAGDSAGANICHYLASRAGSEDL--- 204
           Y+D W +LKWV  H  G G E W+N   DFG++ LAGDSAG NI H+L  RA  E L   
Sbjct: 125 YEDSWDSLKWVLTHITGTGPETWINKHGDFGKVFLAGDSAGGNISHHLTMRAKKEKLCDS 184

Query: 205 ---GMALIHPFFGD---------------GGDNRLWKYLCPGI-SGEDDPSLRPAAEDLR 264
              G+ LIHP+F                  G    W+   P    G DDP L     D  
Sbjct: 185 LISGIILIHPYFWSKTPIDEFEVRDVGKTKGVEGSWRVASPNSKQGVDDPWLNVVGSDPS 244

Query: 265 KLACRRVAVFLAEEDFLKEGGRKYDGELRRSGWRGAAETVEHGGEGHVFHLMKPEYEKAD 313
            L C RV V +A +D     G  Y  +L++SGW G  E +E   EGHVFHL  P  + A 
Sbjct: 245 GLGCGRVLVMVAGDDLFVRQGWCYAEKLKKSGWEGEVEVMETKNEGHVFHLKNPNSDNAR 304

The following BLAST results are available for this feature:

BLAST of MC02g1002 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LMA7	3.9e-64	41.10	Probable carboxylesterase 1 OS=Arabidopsis thaliana OX=3702 GN=CXE1 PE=2 SV=1	[more]
Q9FX94	8.8e-64	40.58	Probable carboxylesterase 5 OS=Arabidopsis thaliana OX=3702 GN=CXE5 PE=2 SV=1	[more]
Q9SMN0	1.1e-63	42.90	Probable carboxylesterase 12 OS=Arabidopsis thaliana OX=3702 GN=CXE12 PE=1 SV=1	[more]
Q9SMM9	4.8e-62	41.12	Probable carboxylesterase 13 OS=Arabidopsis thaliana OX=3702 GN=CXE13 PE=1 SV=1	[more]
Q9ZQ91	5.3e-61	40.97	Probable carboxylesterase 7 OS=Arabidopsis thaliana OX=3702 GN=CXE7 PE=2 SV=1	[more]

BLAST of MC02g1002 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004145358.2	9.11e-146	69.16	2-hydroxyisoflavanone dehydratase [Cucumis sativus] >KGN61726.1 hypothetical pro...	[more]
XP_008459211.1	2.34e-141	67.86	PREDICTED: 2-hydroxyisoflavanone dehydratase-like [Cucumis melo] >KAA0032058.1 2...	[more]
XP_022986577.1	2.08e-134	64.82	probable carboxylesterase 12 [Cucurbita maxima] >XP_022986578.1 probable carboxy...	[more]
XP_023512082.1	2.41e-133	64.82	probable carboxylesterase 12 [Cucurbita pepo subsp. pepo]	[more]
KAG6571158.1	1.39e-132	64.17	putative carboxylesterase 2, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of MC02g1002 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LNZ1	4.41e-146	69.16	Abhydrolase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G2345...	[more]
A0A5D3CP06	1.13e-141	67.86	2-hydroxyisoflavanone dehydratase-like OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A1S3C9R7	1.13e-141	67.86	2-hydroxyisoflavanone dehydratase-like OS=Cucumis melo OX=3656 GN=LOC103498401 P...	[more]
A0A6J1J7Y5	1.01e-134	64.82	probable carboxylesterase 12 OS=Cucurbita maxima OX=3661 GN=LOC111484278 PE=4 SV...	[more]
A0A6J1FTL3	1.35e-132	64.17	probable carboxylesterase 12 OS=Cucurbita moschata OX=3662 GN=LOC111448682 PE=4 ...	[more]

BLAST of MC02g1002 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G19190.1	2.8e-65	41.10	alpha/beta-Hydrolases superfamily protein	[more]
AT1G49660.1	6.2e-65	40.58	carboxyesterase 5	[more]
AT3G48690.1	8.1e-65	42.90	alpha/beta-Hydrolases superfamily protein	[more]
AT3G48700.1	3.4e-63	41.12	carboxyesterase 13	[more]
AT2G03550.1	3.8e-62	40.97	alpha/beta-Hydrolases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013094	Alpha/beta hydrolase fold-3	PFAM	PF07859	Abhydrolase_3	coord: 95..292 e-value: 6.6E-37 score: 127.4
IPR029058	Alpha/Beta hydrolase fold	GENE3D	3.40.50.1820	alpha/beta hydrolase	coord: 36..312 e-value: 2.3E-64 score: 219.9
IPR029058	Alpha/Beta hydrolase fold	SUPERFAMILY	53474	alpha/beta-Hydrolases	coord: 32..311
None	No IPR available	PANTHER	PTHR23024	ARYLACETAMIDE DEACETYLASE	coord: 19..312
None	No IPR available	PANTHER	PTHR23024:SF514	CARBOXYLESTERASE 12-RELATED	coord: 19..312
IPR033140	Lipase, GDXG, putative serine active site	PROSITE	PS01174	LIPASE_GDXG_SER	coord: 177..189

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC02g1002.1	MC02g1002.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016787	hydrolase activity