MC02g0966 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC02g0966
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionRING/U-box superfamily protein
LocationMC02: 7745680 .. 7750833 (-)
RNA-Seq ExpressionMC02g0966
SyntenyMC02g0966
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
TCAACCAGATATTCGGAACCGGCGTCGTTTTCGACAATCTTCGAAACACAAAACGACGGCGTTTTGATCCGTGCTCTCGATTCTCCACCAAAACACCGAGCTCTCGCTTCTCACAAATTTGCTCTCTTAATTTCCATTTTTGTCTGACGCCTCCTATTAGTCGTTGTCCCTCTCACTCCCCTCGCCGTATTATCCAACGATCTCGCCGGAGGGAAACGTTCCCCGGCGTTTCCCGATTCCCCGGATCCGATTTGCTCATCGAATCTCATCATCAATTACCATTAACCTCTGCCTTTTCTTCTTTCTTTTTTTCCCTTTTTCATTTTTCCATCTGACTTCAATTTGTCTCCCTCTTTTCCCAATTCACTCGCGTATTCCTTTCTCCAATTCCCCAATGGAGGACTGCTTCAGCAGCGATGAGGACTACTACGACTCCGATCGAGAGTCTCTTCACGGTCTCGAGAACGAGGAGTCCGAAATTCAGTGGGCCCCCGAGGGTCCCGCGACTAAGGTATTCAATTTATTGGGAGGGGAATTGCAATTTGCAATTTGCAATTTGTTTGTTACCCGGTTCGTGGATTGAACCATTATTTTTTTATTTTAATTCTTGATTCAATTTACGAAATTTTGTGATGGCAGGGTGTGGATTTGTTGTGTAGAAATATACATGATTTATTTTGCTTTCGCGTGCTGAAAATTTGGGTTTTGCTACATTTGGGCTCTTGTTGTATTTGTTATTATATGTTTTCTCTTTTCCGAAAAAGAGAGAATTTCTTCATGTTTGTGATGATGGGGTGGCGTAAGATGGTGTCATAATGCCGTAATTTCGTTCGTGTTAAGATTTACATGCATTTTCTGATTGATCATACTTATGAATATCATTCTTGTTGTTTTCAGGTTATTACTAAAGAATCACTCCTTGCGGCACAGGTTTAACACCACTTCCTAACTTCATATAATAGACCCTTTTTTTCTCATTTTATCATCAAATTGGATGCTTAGGTTCTTTTTCGTTATTTGTATTTGATGTTGGAGTTGTTTTGTTTTCATGTAGAAGGAGGACATGCGTAGAGTAATGGACTTGCTATCATTGAGGGAATACCACGCTCGGACCTTACTCATTCATTACCGATGGGATGTAGAGAAATTGTCTGCAGTATTTGTGGAGAAAGGGAAAAGTTACTTGTTTGCTACAGCTGGTGTTACTACGATTGAGCAACAAAGTAATAGTTCCTCTGAGCCTTCCTCAATGGTGATGTGCGATATCTGCATGGAGGAAGTACATGGCAATGAAGCAACAAGGATGGACTGTGGTCATTGCTTTTGCAATAACTGTAAGCATTTTTCTATATAATTTTGTTTGTTTTTTAATAAAACTTGTTCCCATAATCTTTCTACATATACTGAGGAATGCATACTTTTGGTGTTCCATAAAAAGATTTTTTCAAGGGCCTTATGAACTGATATTATAAAGATTGGCTGTGATATTGTTAATGTTAGTTAAGCCACTGTATGGAATGAAAATTTGAAGTGTTTATAGAAATTACTAATAATTGTCTGAGCTGAATTGTTCCTTATGTTATTGAAAATATGAAACAGTTATCCTGAGATCAAATTTAATTCATTTATTGCTTCAGGTTGGACTGAACATTTTATTGTGAAAATAAATGAGGGCCAGAGTCGGAGAATCAGGTGCATGGCCCACAAATGTAATGCTATCTGTGATGAAGCAGTTGTTAGGAATCTAGTTAGTAAAAGGCATCCAGATCTTGCCAACAAATTTGATCGTTTTCTTCTTGAGTCGTACATTGAGGATAATAAAAGAGTCAAGTGGTGTCCAAGTACCCCTCATTGTGGCAATGCTATACGCGTGGAGGATGATGAGTTTTGTGAAGTAGAATGTTCGTGTGGTCTTCAGTTTTGTTTCAGCTGCTTATCAGAAGCTCACTCGCCATGTTCATGTTTGATGTGGGAACTTTGGATTAAGAAGTGCCGAGATGAATCTGAAACAGTTAACTGGATTACGGTACATACAAAGCCCTGTCCAAAGTGCCACAAACCTGTCGAGAAGAATGGTGGCTGTAACCTCGTCAGCTGCATCTGTGGTCAAGCATTTTGGTAGGATTCTACTATTTTCTAGTCATATCTCTTTCATATATTGCTGAGACTTTGAGGCAGATAGCTCACTGTCATATATTGCTGAGACTGCTGACACTGCAAGTATCCGCCAGTTTGGTATTCTTCAGATACTTGTAGTTCATACTTAGATTTGATATGAAACAAACGTTCCTCTATTCAACGAGTTTTCAAATAAATAGTTGGACTTGTGCATATCAGATTAACGTGCTTGTCTGACTCTTTTGAGCTCTCTGTGTGAATGTGCTGACATGTAGCAGTTAATGCATGCCAGTCTTTTCTAGGAAATAACTTTAATTGTTTTCCCTAGTGTGGTTAATTTTTTTTTAATTTCCCCAAGTGTTAAAGAGTAAAGGCATCATACTTCCTCAGTGTCATGTTATGGATGTTTGATGTGGGCTGTGGCTAATTTGAAGAACTTTCGAAATTATGATAATTTTGACTTAAAATTTAGACCTAAATTCTTCTTTGAAAGCATAATGGATTTTAGCTGCATTGAATAAAATTTTCTGCTTTTCGTTTGGTTGAAAATACCAAAAACCTCTCATGTCTCCCGAGTCCGGCCTTGGGTGGTTGTAGGTACCCCTGGGTATAGGGGAGCAAAGCTCCAACTCTCAATTATTAAAAAAAATGATATTTCCTTTTTATTTATTTATTTATTTTTACCTTTTTGAGCTTTTCAAATTATCAATAGAATTTTTAATTTTCTGATCTCATGGTTTGTCTTCTCATTCATATGTATCTTGGATGATTTCGATGCATACTGGATTCATTTAGAATGAGCTCTATGAAGGCATGCAACTAAGTTAATTTGATGGAATTTAGTAATATCTAAATCCATCGTTTTTTTTTTATTTAAACTTAAAATATAAAAAAATTAATTGAATATCATGGTTTGAAAATAAAATAATGTTTTCACTTTAATATGTTTCTGATATTCACTTGAGTGAGTTTAATATAATGTATTTTATCATTTTAACCTTATTTTAGTTTTAGAATCTTTAAAAATATGTTTTCCATGATTTAGGATATAAAGGTAGTGAGGTGTGACGTGTGAAAGATGGGAATTTTTGCCTTTAACTTACCAAATAACTTAAACTTTTTTCAATTTAAATATAAATTCGTAAGAGTCGAGCATCTTGTACTTAATACTAAGTTTTCTGATAGTAGAGCTCTGTTTATTTTTCTACTTGTTTTATACAAAGAAATTCATTTCAGAAATAGTTTTGCTATCACTTATCTAACAATTCTTAATTTCATCTCTTAACTTGCTGCTTGTTTCTTTTCCAGCTATTTATTATTCTGCCCTTATTTTAGTTTTATCCAGTGTGGTCTAAGTATCTACTTCCCATCCTCCTGAAACAGTTGGCTGTGTGGTGGAGCTACCGGTCGAGAACATACTTGGTCGAGTATTTCTGGACACAGTTGTGGGCGTTACAAAGAAGAATCAGAACAGAAAGCTGAACGAGCAAAGCGGGATTTGTACCGCTATATGCATTATCATAATCGGTACAAAGCTCATACAGATTCTTTTAAGCTTGAAAGCAAATTGAAGGAATCAATACAAGAGAAGATCTCAATTTCTGAAGAGAGGGAATCCATGCTCAGAGATTTTAGTTGGGTAAACAATGGACTGTACAGACTATTCAGATCAAGGCGGGTTCTTTCGTATTCATACCCATTTGCATTTTATATGTTTGGGGATGAGCTCTTCAAAGATGAGATGACACAGGAGGAAAGAGAGATAAAGCAACATCTGTTCGAGGACCAGCAACAGCAGCTTGAGGCAAATGTTGAGAAACTGTCCAAGTTCTTAGAGGAACCGTTTGATCAATACACTAAAGATAAGGTTATGGAGATAAGGATGCAAGTCATCAATCTGTCAGTTATCACTGATACCCTCTGTAAGAAGATGTAAGTGTTCTCGAACAATCTCTTCTTCTGAAATGAGTGTAAATCTCTTGGAACTAATGTCTTATTCTCAAACAGGTACGACTGTATTGAGAATGATCTTTTGGGTTCTCTCGAACTGGGTATTCACAATATTGCTCCTTACAAGTCGAAGGGCATCGAGAAGGCATTGGAACTTTCTGCTTCTTGGAACAGCAGAGCAAATACTACAGACAAATATCTGCCATCAGACTGTGGTACAAGTGGTAAAGTGGAGAGTACTTTTCTACACTTTCTTATTCATCCCTGTCATTTCTAAAATTTCATCTGTTCTTCTAAAAAGATGCATTTATTATCTGCAAATCTAAATCAATTAATGAAAAACATATTGCTTGGTCTGTCCATAAGATAAATGTTTATGTTGGGCCTATTTGATGTGGAGTTCTTTGAATTCTTTGTAGTCTGTTTCTGAGACATTGTGTCAGAGATACTACATCTATAAAGCTTTTGAAAGATACTGGGTAAAAAAGTTTTACTGATACTACAAAATGCAGGGGGTTTGTCAGAACGCGATCGATCCACTGGCTTGAATTCTGAGGAGAATGGATGCTCTTCTAGGAAGCGTGCCAGAGCAGAAGGTGTTGCTGGCAACTTTTTTGATCTAAACTTGCCTGCTGGGGTGGTTGATAGGAACTGATAGATATCTGGAAGTTGCGTGGTTATAAAGTACAGTTGTACAGATCTGACATAAAACATTAGCTTCTTTAGCGTGGGGCCGGGTAATCGAGGATTGGTATCCCAGGTGCATTTAACGCTTCACTCTTTGTTAATCATCTTTGTCTCGGGTTGAATATATTCTCTTCGGCTGAGCTATTTTGAAAGAGAATCTCGGAAGTTGATGTTCTTTGTTTCCATTTTCTTTTGACGAATCGCCAAATTGTACATAGCCGAAATCGGGCTTTGATGATGGTATTTGGAATGTAAATGCTAAGTTCTCAGCTTGAGACAAACATATGGTATATGCATTTTGAGATGGTCTAATATTTTCCGACTGGAAGTGAAGTTTCAATGTTTTATTTATTTGATGTGTTCTGAAGTTTATGCTGTGTGCCATAAACTAAATGTGTG

mRNA sequence

TCAACCAGATATTCGGAACCGGCGTCGTTTTCGACAATCTTCGAAACACAAAACGACGGCGTTTTGATCCGTGCTCTCGATTCTCCACCAAAACACCGAGCTCTCGCTTCTCACAAATTTGCTCTCTTAATTTCCATTTTTGTCTGACGCCTCCTATTAGTCGTTGTCCCTCTCACTCCCCTCGCCGTATTATCCAACGATCTCGCCGGAGGGAAACGTTCCCCGGCGTTTCCCGATTCCCCGGATCCGATTTGCTCATCGAATCTCATCATCAATTACCATTAACCTCTGCCTTTTCTTCTTTCTTTTTTTCCCTTTTTCATTTTTCCATCTGACTTCAATTTGTCTCCCTCTTTTCCCAATTCACTCGCGTATTCCTTTCTCCAATTCCCCAATGGAGGACTGCTTCAGCAGCGATGAGGACTACTACGACTCCGATCGAGAGTCTCTTCACGGTCTCGAGAACGAGGAGTCCGAAATTCAGTGGGCCCCCGAGGGTCCCGCGACTAAGGTTATTACTAAAGAATCACTCCTTGCGGCACAGAAGGAGGACATGCGTAGAGTAATGGACTTGCTATCATTGAGGGAATACCACGCTCGGACCTTACTCATTCATTACCGATGGGATGTAGAGAAATTGTCTGCAGTATTTGTGGAGAAAGGGAAAAGTTACTTGTTTGCTACAGCTGGTGTTACTACGATTGAGCAACAAAGTAATAGTTCCTCTGAGCCTTCCTCAATGGTGATGTGCGATATCTGCATGGAGGAAGTACATGGCAATGAAGCAACAAGGATGGACTGTGGTCATTGCTTTTGCAATAACTGTTGGACTGAACATTTTATTGTGAAAATAAATGAGGGCCAGAGTCGGAGAATCAGGTGCATGGCCCACAAATGTAATGCTATCTGTGATGAAGCAGTTGTTAGGAATCTAGTTAGTAAAAGGCATCCAGATCTTGCCAACAAATTTGATCGTTTTCTTCTTGAGTCGTACATTGAGGATAATAAAAGAGTCAAGTGGTGTCCAAGTACCCCTCATTGTGGCAATGCTATACGCGTGGAGGATGATGAGTTTTGTGAAGTAGAATGTTCGTGTGGTCTTCAGTTTTGTTTCAGCTGCTTATCAGAAGCTCACTCGCCATGTTCATGTTTGATGTGGGAACTTTGGATTAAGAAGTGCCGAGATGAATCTGAAACAGTTAACTGGATTACGGTACATACAAAGCCCTGTCCAAAGTGCCACAAACCTGTCGAGAAGAATGGTGGCTGTAACCTCGTCAGCTGCATCTGTGGTCAAGCATTTTGTTGGCTGTGTGGTGGAGCTACCGGTCGAGAACATACTTGGTCGAGTATTTCTGGACACAGTTGTGGGCGTTACAAAGAAGAATCAGAACAGAAAGCTGAACGAGCAAAGCGGGATTTGTACCGCTATATGCATTATCATAATCGGTACAAAGCTCATACAGATTCTTTTAAGCTTGAAAGCAAATTGAAGGAATCAATACAAGAGAAGATCTCAATTTCTGAAGAGAGGGAATCCATGCTCAGAGATTTTAGTTGGGTAAACAATGGACTGTACAGACTATTCAGATCAAGGCGGGTTCTTTCGTATTCATACCCATTTGCATTTTATATGTTTGGGGATGAGCTCTTCAAAGATGAGATGACACAGGAGGAAAGAGAGATAAAGCAACATCTGTTCGAGGACCAGCAACAGCAGCTTGAGGCAAATGTTGAGAAACTGTCCAAGTTCTTAGAGGAACCGTTTGATCAATACACTAAAGATAAGGTTATGGAGATAAGGATGCAAGTCATCAATCTGTCAGTTATCACTGATACCCTCTGTAAGAAGATGTACGACTGTATTGAGAATGATCTTTTGGGTTCTCTCGAACTGGGTATTCACAATATTGCTCCTTACAAGTCGAAGGGCATCGAGAAGGCATTGGAACTTTCTGCTTCTTGGAACAGCAGAGCAAATACTACAGACAAATATCTGCCATCAGACTGTGGTACAAGTGGGGGTTTGTCAGAACGCGATCGATCCACTGGCTTGAATTCTGAGGAGAATGGATGCTCTTCTAGGAAGCGTGCCAGAGCAGAAGGTGTTGCTGGCAACTTTTTTGATCTAAACTTGCCTGCTGGGGTGGTTGATAGGAACTGATAGATATCTGGAAGTTGCGTGGTTATAAAGTACAGTTGTACAGATCTGACATAAAACATTAGCTTCTTTAGCGTGGGGCCGGGTAATCGAGGATTGGTATCCCAGGTGCATTTAACGCTTCACTCTTTGTTAATCATCTTTGTCTCGGGTTGAATATATTCTCTTCGGCTGAGCTATTTTGAAAGAGAATCTCGGAAGTTGATGTTCTTTGTTTCCATTTTCTTTTGACGAATCGCCAAATTGTACATAGCCGAAATCGGGCTTTGATGATGGTATTTGGAATGTAAATGCTAAGTTCTCAGCTTGAGACAAACATATGGTATATGCATTTTGAGATGGTCTAATATTTTCCGACTGGAAGTGAAGTTTCAATGTTTTATTTATTTGATGTGTTCTGAAGTTTATGCTGTGTGCCATAAACTAAATGTGTG

Coding sequence (CDS)

ATGGAGGACTGCTTCAGCAGCGATGAGGACTACTACGACTCCGATCGAGAGTCTCTTCACGGTCTCGAGAACGAGGAGTCCGAAATTCAGTGGGCCCCCGAGGGTCCCGCGACTAAGGTTATTACTAAAGAATCACTCCTTGCGGCACAGAAGGAGGACATGCGTAGAGTAATGGACTTGCTATCATTGAGGGAATACCACGCTCGGACCTTACTCATTCATTACCGATGGGATGTAGAGAAATTGTCTGCAGTATTTGTGGAGAAAGGGAAAAGTTACTTGTTTGCTACAGCTGGTGTTACTACGATTGAGCAACAAAGTAATAGTTCCTCTGAGCCTTCCTCAATGGTGATGTGCGATATCTGCATGGAGGAAGTACATGGCAATGAAGCAACAAGGATGGACTGTGGTCATTGCTTTTGCAATAACTGTTGGACTGAACATTTTATTGTGAAAATAAATGAGGGCCAGAGTCGGAGAATCAGGTGCATGGCCCACAAATGTAATGCTATCTGTGATGAAGCAGTTGTTAGGAATCTAGTTAGTAAAAGGCATCCAGATCTTGCCAACAAATTTGATCGTTTTCTTCTTGAGTCGTACATTGAGGATAATAAAAGAGTCAAGTGGTGTCCAAGTACCCCTCATTGTGGCAATGCTATACGCGTGGAGGATGATGAGTTTTGTGAAGTAGAATGTTCGTGTGGTCTTCAGTTTTGTTTCAGCTGCTTATCAGAAGCTCACTCGCCATGTTCATGTTTGATGTGGGAACTTTGGATTAAGAAGTGCCGAGATGAATCTGAAACAGTTAACTGGATTACGGTACATACAAAGCCCTGTCCAAAGTGCCACAAACCTGTCGAGAAGAATGGTGGCTGTAACCTCGTCAGCTGCATCTGTGGTCAAGCATTTTGTTGGCTGTGTGGTGGAGCTACCGGTCGAGAACATACTTGGTCGAGTATTTCTGGACACAGTTGTGGGCGTTACAAAGAAGAATCAGAACAGAAAGCTGAACGAGCAAAGCGGGATTTGTACCGCTATATGCATTATCATAATCGGTACAAAGCTCATACAGATTCTTTTAAGCTTGAAAGCAAATTGAAGGAATCAATACAAGAGAAGATCTCAATTTCTGAAGAGAGGGAATCCATGCTCAGAGATTTTAGTTGGGTAAACAATGGACTGTACAGACTATTCAGATCAAGGCGGGTTCTTTCGTATTCATACCCATTTGCATTTTATATGTTTGGGGATGAGCTCTTCAAAGATGAGATGACACAGGAGGAAAGAGAGATAAAGCAACATCTGTTCGAGGACCAGCAACAGCAGCTTGAGGCAAATGTTGAGAAACTGTCCAAGTTCTTAGAGGAACCGTTTGATCAATACACTAAAGATAAGGTTATGGAGATAAGGATGCAAGTCATCAATCTGTCAGTTATCACTGATACCCTCTGTAAGAAGATGTACGACTGTATTGAGAATGATCTTTTGGGTTCTCTCGAACTGGGTATTCACAATATTGCTCCTTACAAGTCGAAGGGCATCGAGAAGGCATTGGAACTTTCTGCTTCTTGGAACAGCAGAGCAAATACTACAGACAAATATCTGCCATCAGACTGTGGTACAAGTGGGGGTTTGTCAGAACGCGATCGATCCACTGGCTTGAATTCTGAGGAGAATGGATGCTCTTCTAGGAAGCGTGCCAGAGCAGAAGGTGTTGCTGGCAACTTTTTTGATCTAAACTTGCCTGCTGGGGTGGTTGATAGGAACTGA

Protein sequence

MEDCFSSDEDYYDSDRESLHGLENEESEIQWAPEGPATKVITKESLLAAQKEDMRRVMDLLSLREYHARTLLIHYRWDVEKLSAVFVEKGKSYLFATAGVTTIEQQSNSSSEPSSMVMCDICMEEVHGNEATRMDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRNLVSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIRVEDDEFCEVECSCGLQFCFSCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICGQAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSFKLESKLKESIQEKISISEERESMLRDFSWVNNGLYRLFRSRRVLSYSYPFAFYMFGDELFKDEMTQEEREIKQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDKVMEIRMQVINLSVITDTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSASWNSRANTTDKYLPSDCGTSGGLSERDRSTGLNSEENGCSSRKRARAEGVAGNFFDLNLPAGVVDRN
Homology
BLAST of MC02g0966 vs. ExPASy Swiss-Prot
Match: Q949V6 (Probable E3 ubiquitin-protein ligase ARI1 OS=Arabidopsis thaliana OX=3702 GN=ARI1 PE=2 SV=1)

HSP 1 Score: 862.8 bits (2228), Expect = 2.2e-249
Identity = 422/599 (70.45%), Postives = 505/599 (84.31%), Query Frame = 0

Query: 1   MEDCFSSDED--YYDSDRESLHGLENEESEIQ-WAPEGPATKVITKESLLAAQKEDMRRV 60
           M+D FS++E+  YY SD++SL G++NEESE+Q  + +   T+VIT+ESLLAAQ+ED+ RV
Sbjct: 1   MDDYFSAEEEACYYSSDQDSLDGIDNEESELQPLSSKRSNTQVITQESLLAAQREDLLRV 60

Query: 61  MDLLSLREYHARTLLIHYRWDVEKLSAVFVEKGKSYLFATAGVTTIEQQSNSSSEP-SSM 120
           M+LLS++E+HARTLLIHY+WDVEKL AVFVEKGK  LF+ AGVT  + Q  +SS P SS 
Sbjct: 61  MELLSIKEHHARTLLIHYQWDVEKLFAVFVEKGKDSLFSGAGVTVFDYQYGNSSFPQSSQ 120

Query: 121 VMCDICMEEVHGNEATRMDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAV 180
           + CD+CME++ G+  TRMDCGHCFCNNCWTEHF V+INEGQS+RIRCMAH+CNAICDE +
Sbjct: 121 MSCDVCMEDLPGDHMTRMDCGHCFCNNCWTEHFTVQINEGQSKRIRCMAHQCNAICDEDI 180

Query: 181 VRNLVSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIRVEDDEFCEVECSCGL 240
           VR+LVSK+ PDLA KFDR+LLESYIEDN+ VKWCPSTPHCGNAIR EDD+ CEVECSCGL
Sbjct: 181 VRSLVSKKRPDLAAKFDRYLLESYIEDNRMVKWCPSTPHCGNAIRAEDDKLCEVECSCGL 240

Query: 241 QFCFSCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVS 300
           QFCFSCL +AHSPCSCLMWELW KKCRDESET+NWITVHTK CPKC+KPVEKNGGCNLV 
Sbjct: 241 QFCFSCLCQAHSPCSCLMWELWRKKCRDESETINWITVHTKLCPKCYKPVEKNGGCNLVR 300

Query: 301 CICGQAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAH 360
           CICGQ FCWLCGGATG +HT+ SI+GHSCGRY+++ E++ ERAKRDL RY HYH+RYKAH
Sbjct: 301 CICGQCFCWLCGGATGSDHTYRSIAGHSCGRYQDDKEKQMERAKRDLNRYTHYHHRYKAH 360

Query: 361 TDSFKLESKLKESIQEKISISEERESMLRDFSWVNNGLYRLFRSRRVLSYSYPFAFYMFG 420
           TDS KLE KL+++I EK+S SE+RE  L+DFSWV NGL RLFRSRRVLSYSY FA+YMFG
Sbjct: 361 TDSSKLEDKLRDTIHEKVSKSEKRELKLKDFSWVTNGLDRLFRSRRVLSYSYAFAYYMFG 420

Query: 421 DELFKDEMTQEEREIKQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDKVMEIRMQVINL 480
           +E+FKDEMT EEREIK++LFEDQQQQLE+NVEKLS+FLEEPFD+++ DKVM IR+Q+INL
Sbjct: 421 EEMFKDEMTPEEREIKKNLFEDQQQQLESNVEKLSQFLEEPFDEFSNDKVMAIRIQIINL 480

Query: 481 SVITDTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSASWNSRANTTDKYLP 540
           SV  DTLCKKMY+CIENDLLGSL+LGIHNI+PY+SKGIE+A +  ASWNS+    DK+ P
Sbjct: 481 SVAVDTLCKKMYECIENDLLGSLQLGIHNISPYRSKGIEQAAQFYASWNSK--DADKFQP 540

Query: 541 SDCGTSGGLSERDRSTGLNSEENG--CSSRKRARAEGVAGN----FFDLNLPAGVVDRN 590
            D GTSG  S  ++++G  S E+    SS+KR + EG   N      DLNLPA  VD+N
Sbjct: 541 LDSGTSGVTSRPEQASGSRSSEDTICSSSQKRPKKEGSFLNNKVTLLDLNLPADFVDQN 597

BLAST of MC02g0966 vs. ExPASy Swiss-Prot
Match: Q84RR2 (Probable E3 ubiquitin-protein ligase ARI2 OS=Arabidopsis thaliana OX=3702 GN=ARI2 PE=2 SV=1)

HSP 1 Score: 852.0 bits (2200), Expect = 3.9e-246
Identity = 427/597 (71.52%), Postives = 488/597 (81.74%), Query Frame = 0

Query: 1   MEDCFSSDED--YYDSDRESLHGLENEES-EIQWAPEGPATKVITKESLLAAQKEDMRRV 60
           M+D  S +E+  YY SD+ESL+G++N+ES  I  +      KVITKESLLAAQ+ED+RRV
Sbjct: 1   MDDNLSGEEEDYYYSSDQESLNGIDNDESVSIPVSSRSNTVKVITKESLLAAQREDLRRV 60

Query: 61  MDLLSLREYHARTLLIHYRWDVEKLSAVFVEKGKSYLFATAGVTTIEQQS--NSSSEPSS 120
           M+LLS++E+HARTLLIHYRWDVEKL AV VEKGK  LF+ AGVT +E QS  +S S  SS
Sbjct: 61  MELLSVKEHHARTLLIHYRWDVEKLFAVLVEKGKDSLFSGAGVTLLENQSCDSSVSGSSS 120

Query: 121 MVMCDICMEEVHGNEATRMDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEA 180
           M+ CDIC+E+V G + TRMDCGH FCNNCWT HF VKINEGQS+RI CMAHKCNAICDE 
Sbjct: 121 MMSCDICVEDVPGYQLTRMDCGHSFCNNCWTGHFTVKINEGQSKRIICMAHKCNAICDED 180

Query: 181 VVRNLVSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIRVEDDEFCEVECSCG 240
           VVR LVSK  PDLA KFDRFLLESYIEDNK VKWCPSTPHCGNAIRVEDDE CEVECSCG
Sbjct: 181 VVRALVSKSQPDLAEKFDRFLLESYIEDNKMVKWCPSTPHCGNAIRVEDDELCEVECSCG 240

Query: 241 LQFCFSCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLV 300
           LQFCFSC S+AHSPCSC+MWELW KKC DESETVNWITVHTKPCPKCHKPVEKNGGCNLV
Sbjct: 241 LQFCFSCSSQAHSPCSCVMWELWRKKCFDESETVNWITVHTKPCPKCHKPVEKNGGCNLV 300

Query: 301 SCICGQAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKA 360
           +C+C Q+FCWLCG ATGR+HTW+ ISGHSCGR++E+ E++ ERAKRDL RYMHYHNRYKA
Sbjct: 301 TCLCRQSFCWLCGEATGRDHTWARISGHSCGRFQEDKEKQMERAKRDLKRYMHYHNRYKA 360

Query: 361 HTDSFKLESKLKESIQEKISISEERESMLRDFSWVNNGLYRLFRSRRVLSYSYPFAFYMF 420
           H DS KLE+KL  +I +K+SISE+RE  L+DFSW  NGL+RLFRSRRVLSYSYPFAFYMF
Sbjct: 361 HIDSSKLEAKLSNNISKKVSISEKRELQLKDFSWATNGLHRLFRSRRVLSYSYPFAFYMF 420

Query: 421 GDELFKDEMTQEEREIKQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDKVMEIRMQVIN 480
           GDELFKDEM+ EEREIKQ+LFEDQQQQLEANVEKLSKFLEEPFDQ+  DKVM+IR+QVIN
Sbjct: 421 GDELFKDEMSSEEREIKQNLFEDQQQQLEANVEKLSKFLEEPFDQFADDKVMQIRIQVIN 480

Query: 481 LSVITDTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSASWNSRANTTDKYL 540
           LSV  DTLC+ MY+CIENDLLGSL+LGIHNI PY+S GIE+A +  +S NS+        
Sbjct: 481 LSVAVDTLCENMYECIENDLLGSLQLGIHNITPYRSNGIERASDFYSSQNSKEAVGQS-- 540

Query: 541 PSDCGTSGGLSERDRSTGLNSEENGCSSRKRARAEGVAGN----FFDLNLPAGVVDR 589
            SDCG +  L +   S    SE+  CSS KRAR +    N      DLNLPA  ++R
Sbjct: 541 -SDCGWTSRLDQALESG--KSEDTSCSSGKRARIDESYRNSQTTLLDLNLPAEAIER 592

BLAST of MC02g0966 vs. ExPASy Swiss-Prot
Match: Q9LVX0 (Probable E3 ubiquitin-protein ligase ARI3 OS=Arabidopsis thaliana OX=3702 GN=ARI3 PE=2 SV=1)

HSP 1 Score: 647.5 bits (1669), Expect = 1.4e-184
Identity = 326/551 (59.17%), Postives = 418/551 (75.86%), Query Frame = 0

Query: 2   EDCFSSDEDYYDSDRESLHGLEN-EESEIQWAP---EGPATKVITKESLLAAQKEDMRRV 61
           +D    D+DY + + E+    +N  E+E+   P       ++VI KESL+AAQKE + RV
Sbjct: 3   DDYMMLDDDYGEEEDENYSEDDNYSEAEVDLQPVTSTKSTSQVIKKESLVAAQKEILVRV 62

Query: 62  MDLLSLREYHARTLLIHYRWDVEKLSAVFVEKGKSYLFATAGVTTIEQQSNSSSEPSSMV 121
           M+LLS++E  ARTLLI+Y+W+VEKL +VF ++GK  +F+ AG+T       +S +    +
Sbjct: 63  MELLSVKENQARTLLIYYQWNVEKLFSVFADQGKDRMFSCAGLTVFVPSLVTSKK---TM 122

Query: 122 MCDICME-EVHGNEATRMDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAV 181
            CD+CME ++  N  TRM+CGH FCN+CW  HF VKINEG+S+RI CMAH+C AICDE V
Sbjct: 123 KCDVCMEDDLPSNVMTRMECGHRFCNDCWIGHFTVKINEGESKRILCMAHECKAICDEDV 182

Query: 182 VRNLVSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIRVEDD--EFCEVECSC 241
           VR LVS   P+LA+++DRFL+ESY+EDN  VKWCPS PHCG+AIR  +D  +  EV CSC
Sbjct: 183 VRKLVS---PELADRYDRFLIESYVEDNNMVKWCPSKPHCGSAIRKIEDGHDVVEVGCSC 242

Query: 242 GLQFCFSCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNL 301
           GLQFCFSCLSE+HSPCSCLMW+LW KKC DESETVNWITV+TK CPKC KP++K  GCNL
Sbjct: 243 GLQFCFSCLSESHSPCSCLMWKLWKKKCEDESETVNWITVNTKLCPKCSKPIQKRDGCNL 302

Query: 302 VSCICGQAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYK 361
           ++C CGQ FCWLCG ATGR+HT++SI+GHSCGRYK+E  ++ ERA+RDL RY HYH RYK
Sbjct: 303 MTCKCGQHFCWLCGQATGRDHTYTSIAGHSCGRYKDEKVRQLERAQRDLDRYTHYHYRYK 362

Query: 362 AHTDSFKLESKLKESIQEK-ISISEER-ESMLRDFSWVNNGLYRLFRSRRVLSYSYPFAF 421
           AH DS KLE KL++SI EK +S SE + + + +++SWV + + RLF SRR+LS SYPFAF
Sbjct: 363 AHIDSLKLEDKLRKSILEKAVSNSETKDQKVFKEYSWVTDAVNRLFISRRILSQSYPFAF 422

Query: 422 YMFGDELFKDEMTQEEREIKQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDKVMEIRMQ 481
           YMFG+ELFKDEM+++EREIK++LFEDQQQQLE NVEKLSK LEEPFD+Y  +KV+E+   
Sbjct: 423 YMFGEELFKDEMSEKEREIKKNLFEDQQQQLEGNVEKLSKILEEPFDEYDHEKVVEMMRH 482

Query: 482 VINLSVITDTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSASWNSRANTTD 541
           + NL+ + D LCK+MY+CIEN+LLG ++ G HNIAPY+SKGIE+A E  A          
Sbjct: 483 LTNLTAVVDNLCKEMYECIENELLGPIQFGNHNIAPYRSKGIEQATEFCAE--------- 535

Query: 542 KYLPSDCGTSG 544
               SDCG+SG
Sbjct: 543 ---SSDCGSSG 535

BLAST of MC02g0966 vs. ExPASy Swiss-Prot
Match: Q9LVW9 (Putative E3 ubiquitin-protein ligase ARI4 OS=Arabidopsis thaliana OX=3702 GN=ARI4 PE=5 SV=2)

HSP 1 Score: 644.8 bits (1662), Expect = 9.4e-184
Identity = 322/527 (61.10%), Postives = 406/527 (77.04%), Query Frame = 0

Query: 2   EDCFSSDEDYYDSDRESLHGLENEESEIQWAPEGPATKVITKESLLAAQKEDMRRVMDLL 61
           ++C+ S+ D +D    +      EES++Q + E P ++VITKE+L+AAQKE + +VM+ L
Sbjct: 13  DNCYPSEFDDHDQMCSNA-----EESDLQHSRE-PTSQVITKEALVAAQKEVLVKVMEFL 72

Query: 62  SLREYHARTLLIHYRWDVEKLSAVFVEKGKSYLFATAGVTTIEQQSNSSSEPSSMVMCDI 121
           S+ E  ARTLLI Y+W+V+KL +V+ ++GK  LF+ AG+T  +      S     + CDI
Sbjct: 73  SVTENQARTLLIQYQWNVDKLFSVYTDQGKDVLFSRAGLTVFD-----PSLTKKTMKCDI 132

Query: 122 CMEEVHGNEA-TRMDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRNL 181
           CMEE     A TRM+CGH FCN+CW EHF V+INEG+ +RIRCMA+KCN ICDEA  R L
Sbjct: 133 CMEEDLSKYAMTRMECGHRFCNDCWKEHFTVRINEGEGKRIRCMAYKCNTICDEA--RQL 192

Query: 182 VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIR--VEDDEFCEVECSCGLQF 241
           VS    +LA KFDRFL+ESY+EDN  VKWCPSTPHCGNAIR   +D +  EVECSCGLQF
Sbjct: 193 VS---TELAEKFDRFLIESYVEDNNMVKWCPSTPHCGNAIRNIKDDGDVDEVECSCGLQF 252

Query: 242 CFSCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCI 301
           CFSCLSE+HSPCSCLMW+LW KKC DESETVNW+TV+TK CPKC KP++K  GCN ++C 
Sbjct: 253 CFSCLSESHSPCSCLMWKLWKKKCEDESETVNWMTVNTKLCPKCSKPIQKRDGCNHMTCK 312

Query: 302 CGQAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTD 361
           CGQ FCWLCG ATGR+H++SSI+GHSCGRYKEE  ++ ERA+RDL RY HYH RYKAH D
Sbjct: 313 CGQHFCWLCGQATGRDHSYSSIAGHSCGRYKEEKVRQLERAQRDLDRYTHYHYRYKAHID 372

Query: 362 SFKLESKLKESIQEKISISEER--ESMLRDFSWVNNGLYRLFRSRRVLSYSYPFAFYMFG 421
           S KLE KLK+SI +K  ++ E   + + +++SW+ + + RLFRSRR+LSYSYPF FYMFG
Sbjct: 373 SLKLEDKLKKSILKKAVLNSETKDQKVFKEYSWIIDAVNRLFRSRRILSYSYPFVFYMFG 432

Query: 422 DELFKDEMTQEEREIKQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDKVMEIRMQVINL 481
            ELFKD+M+ EER IK++LFEDQQQQLE NVE+LSK LEEPFD+Y  +KV+E+   + NL
Sbjct: 433 KELFKDDMSDEERNIKKNLFEDQQQQLEGNVERLSKILEEPFDEYDHEKVVEMMRHLTNL 492

Query: 482 SVITDTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSAS 524
           + + D LCK+MY+CIEN+LLG L  GIHNIAPY+SKGIE+A E SAS
Sbjct: 493 TAVVDNLCKEMYECIENELLGPLISGIHNIAPYRSKGIEQAAEFSAS 523

BLAST of MC02g0966 vs. ExPASy Swiss-Prot
Match: Q94981 (E3 ubiquitin-protein ligase ariadne-1 OS=Drosophila melanogaster OX=7227 GN=ari-1 PE=1 SV=2)

HSP 1 Score: 241.1 bits (614), Expect = 3.1e-62
Identity = 148/451 (32.82%), Postives = 230/451 (51.00%), Query Frame = 0

Query: 39  KVITKESLLAAQKEDMRRVMDLLSLREYHARTLLIHYRWDVEKLSAVFVEKGKSYLFATA 98
           KV+T + ++  Q+E +     LL L     R LL H++WD EKL   + +      F  A
Sbjct: 50  KVLTTDEIVQHQREIIDEANLLLKLPTPTTRILLNHFKWDKEKLLEKYFDDNTDEFFKCA 109

Query: 99  GV------TTIEQQSNSSSEPSSMVMCDICMEEVHGNEATRMDCGHCFCNNCWTEHFIVK 158
            V      T   +Q  S S+      C+IC  ++  +    ++CGH FC  CW E+   K
Sbjct: 110 HVINPFNATEAIKQKTSRSQCEE---CEICFSQLPPDSMAGLECGHRFCMPCWHEYLSTK 169

Query: 159 I-NEGQSRRIRCMAHKCNAICDEAVVRNLVSKRHPDLANKFDRFLLESYIEDNKRVKWCP 218
           I  EG  + I C AH C+ + D+  V NLV+     +  K+ + +  S++E N+ ++WCP
Sbjct: 170 IVAEGLGQTISCAAHGCDILVDDVTVANLVTDAR--VRVKYQQLITNSFVECNQLLRWCP 229

Query: 219 STPHCGNAIRVEDDEFCEVECSCGLQFCFSCLSEAHSPCSCLMWELWIKKCRDESETVNW 278
           S   C  A++V   E   V C CG  FCF+C    H P  C   + WIKKC D+SET NW
Sbjct: 230 SV-DCTYAVKVPYAEPRRVHCKCGHVFCFACGENWHDPVKCRWLKKWIKKCDDDSETSNW 289

Query: 279 ITVHTKPCPKCHKPVEKNGGCNLVSC---ICGQAFCWLCGGATGREHTWS--SISGHSCG 338
           I  +TK CP+C   +EK+GGCN + C    C   FCW+C G      +W     S ++C 
Sbjct: 290 IAANTKECPRCSVTIEKDGGCNHMVCKNQNCKNEFCWVCLG------SWEPHGSSWYNCN 349

Query: 339 RYKEESEQKA----ERAKRDLYRYMHYHNRYKAHTDSFKLESKLKESIQEKISISEERES 398
           RY E+  + A    E+ +  L RY+HY+NRY  H  S K E+KL  S+++K+   ++   
Sbjct: 350 RYDEDEAKTARDAQEKLRSSLARYLHYYNRYMNHMQSMKFENKLYASVKQKMEEMQQHNM 409

Query: 399 MLRDFSWVNNGLYRLFRSRRVLSYSYPFAFYMFGDELFKDEMTQEEREIKQHLFEDQQQQ 458
              +  ++   +  L + R+ L Y+Y FA+Y+             ++  +  +FED Q+ 
Sbjct: 410 SWIEVQFLKKAVDILCQCRQTLMYTYVFAYYL-------------KKNNQSMIFEDNQKD 469

Query: 459 LEANVEKLSKFLEEPFDQYTKDKVMEIRMQV 474
           LE+  E LS++LE      T + + +I+ +V
Sbjct: 470 LESATEMLSEYLER---DITSENLADIKQKV 472

BLAST of MC02g0966 vs. NCBI nr
Match: XP_022148613.1 (probable E3 ubiquitin-protein ligase ARI1 isoform X1 [Momordica charantia])

HSP 1 Score: 1202 bits (3111), Expect = 0.0
Identity = 589/589 (100.00%), Postives = 589/589 (100.00%), Query Frame = 0

Query: 1   MEDCFSSDEDYYDSDRESLHGLENEESEIQWAPEGPATKVITKESLLAAQKEDMRRVMDL 60
           MEDCFSSDEDYYDSDRESLHGLENEESEIQWAPEGPATKVITKESLLAAQKEDMRRVMDL
Sbjct: 1   MEDCFSSDEDYYDSDRESLHGLENEESEIQWAPEGPATKVITKESLLAAQKEDMRRVMDL 60

Query: 61  LSLREYHARTLLIHYRWDVEKLSAVFVEKGKSYLFATAGVTTIEQQSNSSSEPSSMVMCD 120
           LSLREYHARTLLIHYRWDVEKLSAVFVEKGKSYLFATAGVTTIEQQSNSSSEPSSMVMCD
Sbjct: 61  LSLREYHARTLLIHYRWDVEKLSAVFVEKGKSYLFATAGVTTIEQQSNSSSEPSSMVMCD 120

Query: 121 ICMEEVHGNEATRMDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRNL 180
           ICMEEVHGNEATRMDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRNL
Sbjct: 121 ICMEEVHGNEATRMDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRNL 180

Query: 181 VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIRVEDDEFCEVECSCGLQFCF 240
           VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIRVEDDEFCEVECSCGLQFCF
Sbjct: 181 VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIRVEDDEFCEVECSCGLQFCF 240

Query: 241 SCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG 300
           SCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG
Sbjct: 241 SCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG 300

Query: 301 QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF 360
           QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF
Sbjct: 301 QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF 360

Query: 361 KLESKLKESIQEKISISEERESMLRDFSWVNNGLYRLFRSRRVLSYSYPFAFYMFGDELF 420
           KLESKLKESIQEKISISEERESMLRDFSWVNNGLYRLFRSRRVLSYSYPFAFYMFGDELF
Sbjct: 361 KLESKLKESIQEKISISEERESMLRDFSWVNNGLYRLFRSRRVLSYSYPFAFYMFGDELF 420

Query: 421 KDEMTQEEREIKQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDKVMEIRMQVINLSVIT 480
           KDEMTQEEREIKQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDKVMEIRMQVINLSVIT
Sbjct: 421 KDEMTQEEREIKQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDKVMEIRMQVINLSVIT 480

Query: 481 DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSASWNSRANTTDKYLPSDCG 540
           DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSASWNSRANTTDKYLPSDCG
Sbjct: 481 DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSASWNSRANTTDKYLPSDCG 540

Query: 541 TSGGLSERDRSTGLNSEENGCSSRKRARAEGVAGNFFDLNLPAGVVDRN 589
           TSGGLSERDRSTGLNSEENGCSSRKRARAEGVAGNFFDLNLPAGVVDRN
Sbjct: 541 TSGGLSERDRSTGLNSEENGCSSRKRARAEGVAGNFFDLNLPAGVVDRN 589

BLAST of MC02g0966 vs. NCBI nr
Match: XP_022148614.1 (probable E3 ubiquitin-protein ligase ARI1 isoform X2 [Momordica charantia])

HSP 1 Score: 1190 bits (3078), Expect = 0.0
Identity = 585/589 (99.32%), Postives = 585/589 (99.32%), Query Frame = 0

Query: 1   MEDCFSSDEDYYDSDRESLHGLENEESEIQWAPEGPATKVITKESLLAAQKEDMRRVMDL 60
           MEDCFSSDEDYYDSDRESLHGLENEESEIQWAPEGPATKVITKESLLAAQKEDMRRVMDL
Sbjct: 1   MEDCFSSDEDYYDSDRESLHGLENEESEIQWAPEGPATKVITKESLLAAQKEDMRRVMDL 60

Query: 61  LSLREYHARTLLIHYRWDVEKLSAVFVEKGKSYLFATAGVTTIEQQSNSSSEPSSMVMCD 120
           LSLREYHARTLLIHYRWDVEKLSAVFVEKGKSYLFATAGVTTIEQQSNSSSEPSSMVMCD
Sbjct: 61  LSLREYHARTLLIHYRWDVEKLSAVFVEKGKSYLFATAGVTTIEQQSNSSSEPSSMVMCD 120

Query: 121 ICMEEVHGNEATRMDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRNL 180
           ICMEEVHGNEATRMDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRNL
Sbjct: 121 ICMEEVHGNEATRMDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRNL 180

Query: 181 VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIRVEDDEFCEVECSCGLQFCF 240
           VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIRVEDDEFCEVECSCGLQFCF
Sbjct: 181 VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIRVEDDEFCEVECSCGLQFCF 240

Query: 241 SCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG 300
           SCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG
Sbjct: 241 SCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG 300

Query: 301 QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF 360
           QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF
Sbjct: 301 QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF 360

Query: 361 KLESKLKESIQEKISISEERESMLRDFSWVNNGLYRLFRSRRVLSYSYPFAFYMFGDELF 420
           KLESKLKESIQEKISISEERESMLRDFSWVNNGLYRLFRSRRVLSYSYPFAFYMFGDELF
Sbjct: 361 KLESKLKESIQEKISISEERESMLRDFSWVNNGLYRLFRSRRVLSYSYPFAFYMFGDELF 420

Query: 421 KDEMTQEEREIKQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDKVMEIRMQVINLSVIT 480
           KDEMTQEEREIKQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDKVMEIRMQVINLSVIT
Sbjct: 421 KDEMTQEEREIKQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDKVMEIRMQVINLSVIT 480

Query: 481 DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSASWNSRANTTDKYLPSDCG 540
           DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSASWNSRANTTDKYLPSDCG
Sbjct: 481 DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSASWNSRANTTDKYLPSDCG 540

Query: 541 TSGGLSERDRSTGLNSEENGCSSRKRARAEGVAGNFFDLNLPAGVVDRN 589
           TS    ERDRSTGLNSEENGCSSRKRARAEGVAGNFFDLNLPAGVVDRN
Sbjct: 541 TS----ERDRSTGLNSEENGCSSRKRARAEGVAGNFFDLNLPAGVVDRN 585

BLAST of MC02g0966 vs. NCBI nr
Match: XP_038901258.1 (probable E3 ubiquitin-protein ligase ARI2 isoform X2 [Benincasa hispida])

HSP 1 Score: 1139 bits (2945), Expect = 0.0
Identity = 556/589 (94.40%), Postives = 571/589 (96.94%), Query Frame = 0

Query: 1   MEDCFSSDEDYYDSDRESLHGLENEESEIQWAPEGPATKVITKESLLAAQKEDMRRVMDL 60
           M+DC SSDEDYYDSDR+SLHG+ENE+SEIQW P+ PATKVITKESLLAAQKEDMRRVMDL
Sbjct: 1   MDDCLSSDEDYYDSDRDSLHGIENEDSEIQWVPKAPATKVITKESLLAAQKEDMRRVMDL 60

Query: 61  LSLREYHARTLLIHYRWDVEKLSAVFVEKGKSYLFATAGVTTIEQQSNSSSEPSSMVMCD 120
           LSLREYHARTLLIHYRWDVEKL AV VEKGKS+LFATAGV+ IE +SNSSSEPSSMVMCD
Sbjct: 61  LSLREYHARTLLIHYRWDVEKLFAVLVEKGKSHLFATAGVSMIENRSNSSSEPSSMVMCD 120

Query: 121 ICMEEVHGNEATRMDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRNL 180
           ICMEEVHGN+ATR+DC HCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRNL
Sbjct: 121 ICMEEVHGNDATRVDCDHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRNL 180

Query: 181 VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIRVEDDEFCEVECSCGLQFCF 240
           VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIRVEDDEFCEVECSCGLQFCF
Sbjct: 181 VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIRVEDDEFCEVECSCGLQFCF 240

Query: 241 SCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG 300
           SCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG
Sbjct: 241 SCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG 300

Query: 301 QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF 360
           QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF
Sbjct: 301 QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF 360

Query: 361 KLESKLKESIQEKISISEERESMLRDFSWVNNGLYRLFRSRRVLSYSYPFAFYMFGDELF 420
           KLESKLKESIQEKISISEERESMLRDFSWVNNGL RLFRSRRVLSYSYPFAFYMFGDELF
Sbjct: 361 KLESKLKESIQEKISISEERESMLRDFSWVNNGLSRLFRSRRVLSYSYPFAFYMFGDELF 420

Query: 421 KDEMTQEEREIKQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDKVMEIRMQVINLSVIT 480
           KDEMT+ EREIKQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKD+VMEIRMQVINLSVIT
Sbjct: 421 KDEMTEAEREIKQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDRVMEIRMQVINLSVIT 480

Query: 481 DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSASWNSRANTTDKYLPSDCG 540
           DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSA WNS+ANT+DKY PSDCG
Sbjct: 481 DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANTSDKYPPSDCG 540

Query: 541 TSGGLSERDRSTGLNSEENGCSSRKRARAEGVAGNFFDLNLPAGVVDRN 589
           TSGGLSE +R T LNSEENGCSSRKRARAE V GNFFDLNLPA VVDRN
Sbjct: 541 TSGGLSECERLTSLNSEENGCSSRKRARAEVVTGNFFDLNLPAEVVDRN 589

BLAST of MC02g0966 vs. NCBI nr
Match: XP_038901257.1 (probable E3 ubiquitin-protein ligase ARI2 isoform X1 [Benincasa hispida])

HSP 1 Score: 1133 bits (2930), Expect = 0.0
Identity = 556/593 (93.76%), Postives = 571/593 (96.29%), Query Frame = 0

Query: 1   MEDCFSSDEDYYDSDRESLHGLENEESEIQWAPEGPATKVITKESLLAAQKEDMRRVMDL 60
           M+DC SSDEDYYDSDR+SLHG+ENE+SEIQW P+ PATKVITKESLLAAQKEDMRRVMDL
Sbjct: 1   MDDCLSSDEDYYDSDRDSLHGIENEDSEIQWVPKAPATKVITKESLLAAQKEDMRRVMDL 60

Query: 61  LSLREYHARTLLIHYRWDVEKLSAVFVEKGKSYLFATAGVTTIEQQSNSSSEPSSMVMCD 120
           LSLREYHARTLLIHYRWDVEKL AV VEKGKS+LFATAGV+ IE +SNSSSEPSSMVMCD
Sbjct: 61  LSLREYHARTLLIHYRWDVEKLFAVLVEKGKSHLFATAGVSMIENRSNSSSEPSSMVMCD 120

Query: 121 ICMEEVHGNEATRMDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRNL 180
           ICMEEVHGN+ATR+DC HCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRNL
Sbjct: 121 ICMEEVHGNDATRVDCDHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRNL 180

Query: 181 VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIRVEDDEFCEVECSCGLQFCF 240
           VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIRVEDDEFCEVECSCGLQFCF
Sbjct: 181 VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIRVEDDEFCEVECSCGLQFCF 240

Query: 241 SCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG 300
           SCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG
Sbjct: 241 SCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG 300

Query: 301 QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF 360
           QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF
Sbjct: 301 QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF 360

Query: 361 KLESKLKESIQEKISISEERESMLRDFSWVNNGLYRLFRSRRVLSYSYPFAFYMFGDELF 420
           KLESKLKESIQEKISISEERESMLRDFSWVNNGL RLFRSRRVLSYSYPFAFYMFGDELF
Sbjct: 361 KLESKLKESIQEKISISEERESMLRDFSWVNNGLSRLFRSRRVLSYSYPFAFYMFGDELF 420

Query: 421 KDEMTQEEREIKQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDKVMEIRMQVINLSVIT 480
           KDEMT+ EREIKQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKD+VMEIRMQVINLSVIT
Sbjct: 421 KDEMTEAEREIKQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDRVMEIRMQVINLSVIT 480

Query: 481 DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSASWNSRANTTDKYLPSDCG 540
           DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSA WNS+ANT+DKY PSDCG
Sbjct: 481 DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANTSDKYPPSDCG 540

Query: 541 TSG----GLSERDRSTGLNSEENGCSSRKRARAEGVAGNFFDLNLPAGVVDRN 589
           TSG    GLSE +R T LNSEENGCSSRKRARAE V GNFFDLNLPA VVDRN
Sbjct: 541 TSGKVERGLSECERLTSLNSEENGCSSRKRARAEVVTGNFFDLNLPAEVVDRN 593

BLAST of MC02g0966 vs. NCBI nr
Match: XP_004153333.1 (probable E3 ubiquitin-protein ligase ARI2 isoform X1 [Cucumis sativus] >KGN61769.1 hypothetical protein Csa_005965 [Cucumis sativus])

HSP 1 Score: 1131 bits (2926), Expect = 0.0
Identity = 555/589 (94.23%), Postives = 570/589 (96.77%), Query Frame = 0

Query: 1   MEDCFSSDEDYYDSDRESLHGLENEESEIQWAPEGPATKVITKESLLAAQKEDMRRVMDL 60
           M+DC SSDEDYYDSDR+SLHGLENE+SEIQ  P+ P+TKVITKESLLAAQKEDMRRVMDL
Sbjct: 1   MDDCISSDEDYYDSDRDSLHGLENEDSEIQRVPKTPSTKVITKESLLAAQKEDMRRVMDL 60

Query: 61  LSLREYHARTLLIHYRWDVEKLSAVFVEKGKSYLFATAGVTTIEQQSNSSSEPSSMVMCD 120
           LSLREYHARTLLIHYRWDVEKL AV VEKGK++LFATAGVT IE +SN+SSE SSMVMCD
Sbjct: 61  LSLREYHARTLLIHYRWDVEKLFAVLVEKGKNHLFATAGVTMIENRSNTSSEASSMVMCD 120

Query: 121 ICMEEVHGNEATRMDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRNL 180
           ICMEEVHGN+ATR+DCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVR L
Sbjct: 121 ICMEEVHGNDATRVDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRTL 180

Query: 181 VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIRVEDDEFCEVECSCGLQFCF 240
           VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIRVEDDEFCEVECSCGLQFCF
Sbjct: 181 VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIRVEDDEFCEVECSCGLQFCF 240

Query: 241 SCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG 300
           SCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG
Sbjct: 241 SCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG 300

Query: 301 QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF 360
           QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF
Sbjct: 301 QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF 360

Query: 361 KLESKLKESIQEKISISEERESMLRDFSWVNNGLYRLFRSRRVLSYSYPFAFYMFGDELF 420
           KLESKLKESIQEKISISEERESMLRDFSWVNNGL RLFRSRRVLSYSYPFAFYMFGDELF
Sbjct: 361 KLESKLKESIQEKISISEERESMLRDFSWVNNGLSRLFRSRRVLSYSYPFAFYMFGDELF 420

Query: 421 KDEMTQEEREIKQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDKVMEIRMQVINLSVIT 480
           KDEMT+ EREIKQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDKVMEIRMQVINLSVIT
Sbjct: 421 KDEMTEAEREIKQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDKVMEIRMQVINLSVIT 480

Query: 481 DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSASWNSRANTTDKYLPSDCG 540
           DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSA WNS+ANT+DKY PSDCG
Sbjct: 481 DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANTSDKYPPSDCG 540

Query: 541 TSGGLSERDRSTGLNSEENGCSSRKRARAEGVAGNFFDLNLPAGVVDRN 589
           TSGGLSE +R T LNSEENGCSSRKRARAE VAGNFFDLNLPA VVDRN
Sbjct: 541 TSGGLSECERLTSLNSEENGCSSRKRARAEVVAGNFFDLNLPAEVVDRN 589

BLAST of MC02g0966 vs. ExPASy TrEMBL
Match: A0A6J1D4K1 (probable E3 ubiquitin-protein ligase ARI1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111017229 PE=3 SV=1)

HSP 1 Score: 1202 bits (3111), Expect = 0.0
Identity = 589/589 (100.00%), Postives = 589/589 (100.00%), Query Frame = 0

Query: 1   MEDCFSSDEDYYDSDRESLHGLENEESEIQWAPEGPATKVITKESLLAAQKEDMRRVMDL 60
           MEDCFSSDEDYYDSDRESLHGLENEESEIQWAPEGPATKVITKESLLAAQKEDMRRVMDL
Sbjct: 1   MEDCFSSDEDYYDSDRESLHGLENEESEIQWAPEGPATKVITKESLLAAQKEDMRRVMDL 60

Query: 61  LSLREYHARTLLIHYRWDVEKLSAVFVEKGKSYLFATAGVTTIEQQSNSSSEPSSMVMCD 120
           LSLREYHARTLLIHYRWDVEKLSAVFVEKGKSYLFATAGVTTIEQQSNSSSEPSSMVMCD
Sbjct: 61  LSLREYHARTLLIHYRWDVEKLSAVFVEKGKSYLFATAGVTTIEQQSNSSSEPSSMVMCD 120

Query: 121 ICMEEVHGNEATRMDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRNL 180
           ICMEEVHGNEATRMDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRNL
Sbjct: 121 ICMEEVHGNEATRMDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRNL 180

Query: 181 VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIRVEDDEFCEVECSCGLQFCF 240
           VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIRVEDDEFCEVECSCGLQFCF
Sbjct: 181 VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIRVEDDEFCEVECSCGLQFCF 240

Query: 241 SCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG 300
           SCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG
Sbjct: 241 SCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG 300

Query: 301 QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF 360
           QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF
Sbjct: 301 QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF 360

Query: 361 KLESKLKESIQEKISISEERESMLRDFSWVNNGLYRLFRSRRVLSYSYPFAFYMFGDELF 420
           KLESKLKESIQEKISISEERESMLRDFSWVNNGLYRLFRSRRVLSYSYPFAFYMFGDELF
Sbjct: 361 KLESKLKESIQEKISISEERESMLRDFSWVNNGLYRLFRSRRVLSYSYPFAFYMFGDELF 420

Query: 421 KDEMTQEEREIKQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDKVMEIRMQVINLSVIT 480
           KDEMTQEEREIKQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDKVMEIRMQVINLSVIT
Sbjct: 421 KDEMTQEEREIKQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDKVMEIRMQVINLSVIT 480

Query: 481 DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSASWNSRANTTDKYLPSDCG 540
           DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSASWNSRANTTDKYLPSDCG
Sbjct: 481 DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSASWNSRANTTDKYLPSDCG 540

Query: 541 TSGGLSERDRSTGLNSEENGCSSRKRARAEGVAGNFFDLNLPAGVVDRN 589
           TSGGLSERDRSTGLNSEENGCSSRKRARAEGVAGNFFDLNLPAGVVDRN
Sbjct: 541 TSGGLSERDRSTGLNSEENGCSSRKRARAEGVAGNFFDLNLPAGVVDRN 589

BLAST of MC02g0966 vs. ExPASy TrEMBL
Match: A0A6J1D4I2 (probable E3 ubiquitin-protein ligase ARI1 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111017229 PE=3 SV=1)

HSP 1 Score: 1190 bits (3078), Expect = 0.0
Identity = 585/589 (99.32%), Postives = 585/589 (99.32%), Query Frame = 0

Query: 1   MEDCFSSDEDYYDSDRESLHGLENEESEIQWAPEGPATKVITKESLLAAQKEDMRRVMDL 60
           MEDCFSSDEDYYDSDRESLHGLENEESEIQWAPEGPATKVITKESLLAAQKEDMRRVMDL
Sbjct: 1   MEDCFSSDEDYYDSDRESLHGLENEESEIQWAPEGPATKVITKESLLAAQKEDMRRVMDL 60

Query: 61  LSLREYHARTLLIHYRWDVEKLSAVFVEKGKSYLFATAGVTTIEQQSNSSSEPSSMVMCD 120
           LSLREYHARTLLIHYRWDVEKLSAVFVEKGKSYLFATAGVTTIEQQSNSSSEPSSMVMCD
Sbjct: 61  LSLREYHARTLLIHYRWDVEKLSAVFVEKGKSYLFATAGVTTIEQQSNSSSEPSSMVMCD 120

Query: 121 ICMEEVHGNEATRMDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRNL 180
           ICMEEVHGNEATRMDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRNL
Sbjct: 121 ICMEEVHGNEATRMDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRNL 180

Query: 181 VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIRVEDDEFCEVECSCGLQFCF 240
           VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIRVEDDEFCEVECSCGLQFCF
Sbjct: 181 VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIRVEDDEFCEVECSCGLQFCF 240

Query: 241 SCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG 300
           SCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG
Sbjct: 241 SCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG 300

Query: 301 QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF 360
           QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF
Sbjct: 301 QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF 360

Query: 361 KLESKLKESIQEKISISEERESMLRDFSWVNNGLYRLFRSRRVLSYSYPFAFYMFGDELF 420
           KLESKLKESIQEKISISEERESMLRDFSWVNNGLYRLFRSRRVLSYSYPFAFYMFGDELF
Sbjct: 361 KLESKLKESIQEKISISEERESMLRDFSWVNNGLYRLFRSRRVLSYSYPFAFYMFGDELF 420

Query: 421 KDEMTQEEREIKQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDKVMEIRMQVINLSVIT 480
           KDEMTQEEREIKQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDKVMEIRMQVINLSVIT
Sbjct: 421 KDEMTQEEREIKQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDKVMEIRMQVINLSVIT 480

Query: 481 DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSASWNSRANTTDKYLPSDCG 540
           DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSASWNSRANTTDKYLPSDCG
Sbjct: 481 DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSASWNSRANTTDKYLPSDCG 540

Query: 541 TSGGLSERDRSTGLNSEENGCSSRKRARAEGVAGNFFDLNLPAGVVDRN 589
           TS    ERDRSTGLNSEENGCSSRKRARAEGVAGNFFDLNLPAGVVDRN
Sbjct: 541 TS----ERDRSTGLNSEENGCSSRKRARAEGVAGNFFDLNLPAGVVDRN 585

BLAST of MC02g0966 vs. ExPASy TrEMBL
Match: A0A0A0LL32 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G238850 PE=3 SV=1)

HSP 1 Score: 1131 bits (2926), Expect = 0.0
Identity = 555/589 (94.23%), Postives = 570/589 (96.77%), Query Frame = 0

Query: 1   MEDCFSSDEDYYDSDRESLHGLENEESEIQWAPEGPATKVITKESLLAAQKEDMRRVMDL 60
           M+DC SSDEDYYDSDR+SLHGLENE+SEIQ  P+ P+TKVITKESLLAAQKEDMRRVMDL
Sbjct: 1   MDDCISSDEDYYDSDRDSLHGLENEDSEIQRVPKTPSTKVITKESLLAAQKEDMRRVMDL 60

Query: 61  LSLREYHARTLLIHYRWDVEKLSAVFVEKGKSYLFATAGVTTIEQQSNSSSEPSSMVMCD 120
           LSLREYHARTLLIHYRWDVEKL AV VEKGK++LFATAGVT IE +SN+SSE SSMVMCD
Sbjct: 61  LSLREYHARTLLIHYRWDVEKLFAVLVEKGKNHLFATAGVTMIENRSNTSSEASSMVMCD 120

Query: 121 ICMEEVHGNEATRMDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRNL 180
           ICMEEVHGN+ATR+DCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVR L
Sbjct: 121 ICMEEVHGNDATRVDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRTL 180

Query: 181 VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIRVEDDEFCEVECSCGLQFCF 240
           VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIRVEDDEFCEVECSCGLQFCF
Sbjct: 181 VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIRVEDDEFCEVECSCGLQFCF 240

Query: 241 SCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG 300
           SCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG
Sbjct: 241 SCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG 300

Query: 301 QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF 360
           QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF
Sbjct: 301 QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF 360

Query: 361 KLESKLKESIQEKISISEERESMLRDFSWVNNGLYRLFRSRRVLSYSYPFAFYMFGDELF 420
           KLESKLKESIQEKISISEERESMLRDFSWVNNGL RLFRSRRVLSYSYPFAFYMFGDELF
Sbjct: 361 KLESKLKESIQEKISISEERESMLRDFSWVNNGLSRLFRSRRVLSYSYPFAFYMFGDELF 420

Query: 421 KDEMTQEEREIKQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDKVMEIRMQVINLSVIT 480
           KDEMT+ EREIKQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDKVMEIRMQVINLSVIT
Sbjct: 421 KDEMTEAEREIKQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDKVMEIRMQVINLSVIT 480

Query: 481 DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSASWNSRANTTDKYLPSDCG 540
           DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSA WNS+ANT+DKY PSDCG
Sbjct: 481 DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANTSDKYPPSDCG 540

Query: 541 TSGGLSERDRSTGLNSEENGCSSRKRARAEGVAGNFFDLNLPAGVVDRN 589
           TSGGLSE +R T LNSEENGCSSRKRARAE VAGNFFDLNLPA VVDRN
Sbjct: 541 TSGGLSECERLTSLNSEENGCSSRKRARAEVVAGNFFDLNLPAEVVDRN 589

BLAST of MC02g0966 vs. ExPASy TrEMBL
Match: A0A5D3CP89 (Putative E3 ubiquitin-protein ligase ARI2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold299G001360 PE=3 SV=1)

HSP 1 Score: 1131 bits (2925), Expect = 0.0
Identity = 555/589 (94.23%), Postives = 569/589 (96.60%), Query Frame = 0

Query: 1   MEDCFSSDEDYYDSDRESLHGLENEESEIQWAPEGPATKVITKESLLAAQKEDMRRVMDL 60
           M+DC SSDEDYYDSDR+SLHGLENE+SEIQ  P+ P+TKVITKESLLAAQKEDMRRVMDL
Sbjct: 1   MDDCISSDEDYYDSDRDSLHGLENEDSEIQRVPKTPSTKVITKESLLAAQKEDMRRVMDL 60

Query: 61  LSLREYHARTLLIHYRWDVEKLSAVFVEKGKSYLFATAGVTTIEQQSNSSSEPSSMVMCD 120
           LSLREYHARTLLIHYRWDVEKL AV VEKGK++LFATAGVT IE +SNSSSE SSMVMCD
Sbjct: 61  LSLREYHARTLLIHYRWDVEKLFAVLVEKGKNHLFATAGVTMIENRSNSSSEASSMVMCD 120

Query: 121 ICMEEVHGNEATRMDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRNL 180
           ICMEEVHGN+ATR+DCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVR L
Sbjct: 121 ICMEEVHGNDATRVDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRTL 180

Query: 181 VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIRVEDDEFCEVECSCGLQFCF 240
           VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIRVEDDEFCEVECSCGLQFCF
Sbjct: 181 VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIRVEDDEFCEVECSCGLQFCF 240

Query: 241 SCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG 300
           SCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG
Sbjct: 241 SCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG 300

Query: 301 QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF 360
           QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF
Sbjct: 301 QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF 360

Query: 361 KLESKLKESIQEKISISEERESMLRDFSWVNNGLYRLFRSRRVLSYSYPFAFYMFGDELF 420
           KLESKLKESIQEKISISEERESMLRDFSWVNNGL RLFRSRRVLSYSYPFAFYMFGDELF
Sbjct: 361 KLESKLKESIQEKISISEERESMLRDFSWVNNGLSRLFRSRRVLSYSYPFAFYMFGDELF 420

Query: 421 KDEMTQEEREIKQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDKVMEIRMQVINLSVIT 480
           KDEMT+ EREIKQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDKVMEIRMQVINLSVIT
Sbjct: 421 KDEMTEAEREIKQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDKVMEIRMQVINLSVIT 480

Query: 481 DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSASWNSRANTTDKYLPSDCG 540
           DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSA WNS+ANT+DKY PSDCG
Sbjct: 481 DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANTSDKYPPSDCG 540

Query: 541 TSGGLSERDRSTGLNSEENGCSSRKRARAEGVAGNFFDLNLPAGVVDRN 589
           TSGGLSE +R T LNSEENGCSSRKRARAE V GNFFDLNLPA VVDRN
Sbjct: 541 TSGGLSECERLTSLNSEENGCSSRKRARAEVVTGNFFDLNLPAEVVDRN 589

BLAST of MC02g0966 vs. ExPASy TrEMBL
Match: A0A1S3C9A1 (probable E3 ubiquitin-protein ligase ARI2 OS=Cucumis melo OX=3656 GN=LOC103498436 PE=3 SV=1)

HSP 1 Score: 1131 bits (2925), Expect = 0.0
Identity = 555/589 (94.23%), Postives = 569/589 (96.60%), Query Frame = 0

Query: 1   MEDCFSSDEDYYDSDRESLHGLENEESEIQWAPEGPATKVITKESLLAAQKEDMRRVMDL 60
           M+DC SSDEDYYDSDR+SLHGLENE+SEIQ  P+ P+TKVITKESLLAAQKEDMRRVMDL
Sbjct: 1   MDDCISSDEDYYDSDRDSLHGLENEDSEIQRVPKTPSTKVITKESLLAAQKEDMRRVMDL 60

Query: 61  LSLREYHARTLLIHYRWDVEKLSAVFVEKGKSYLFATAGVTTIEQQSNSSSEPSSMVMCD 120
           LSLREYHARTLLIHYRWDVEKL AV VEKGK++LFATAGVT IE +SNSSSE SSMVMCD
Sbjct: 61  LSLREYHARTLLIHYRWDVEKLFAVLVEKGKNHLFATAGVTMIENRSNSSSEASSMVMCD 120

Query: 121 ICMEEVHGNEATRMDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRNL 180
           ICMEEVHGN+ATR+DCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVR L
Sbjct: 121 ICMEEVHGNDATRVDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRTL 180

Query: 181 VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIRVEDDEFCEVECSCGLQFCF 240
           VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIRVEDDEFCEVECSCGLQFCF
Sbjct: 181 VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIRVEDDEFCEVECSCGLQFCF 240

Query: 241 SCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG 300
           SCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG
Sbjct: 241 SCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG 300

Query: 301 QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF 360
           QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF
Sbjct: 301 QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF 360

Query: 361 KLESKLKESIQEKISISEERESMLRDFSWVNNGLYRLFRSRRVLSYSYPFAFYMFGDELF 420
           KLESKLKESIQEKISISEERESMLRDFSWVNNGL RLFRSRRVLSYSYPFAFYMFGDELF
Sbjct: 361 KLESKLKESIQEKISISEERESMLRDFSWVNNGLSRLFRSRRVLSYSYPFAFYMFGDELF 420

Query: 421 KDEMTQEEREIKQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDKVMEIRMQVINLSVIT 480
           KDEMT+ EREIKQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDKVMEIRMQVINLSVIT
Sbjct: 421 KDEMTEAEREIKQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDKVMEIRMQVINLSVIT 480

Query: 481 DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSASWNSRANTTDKYLPSDCG 540
           DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSA WNS+ANT+DKY PSDCG
Sbjct: 481 DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANTSDKYPPSDCG 540

Query: 541 TSGGLSERDRSTGLNSEENGCSSRKRARAEGVAGNFFDLNLPAGVVDRN 589
           TSGGLSE +R T LNSEENGCSSRKRARAE V GNFFDLNLPA VVDRN
Sbjct: 541 TSGGLSECERLTSLNSEENGCSSRKRARAEVVTGNFFDLNLPAEVVDRN 589

BLAST of MC02g0966 vs. TAIR 10
Match: AT4G34370.1 (RING/U-box superfamily protein )

HSP 1 Score: 862.8 bits (2228), Expect = 1.6e-250
Identity = 422/599 (70.45%), Postives = 505/599 (84.31%), Query Frame = 0

Query: 1   MEDCFSSDED--YYDSDRESLHGLENEESEIQ-WAPEGPATKVITKESLLAAQKEDMRRV 60
           M+D FS++E+  YY SD++SL G++NEESE+Q  + +   T+VIT+ESLLAAQ+ED+ RV
Sbjct: 1   MDDYFSAEEEACYYSSDQDSLDGIDNEESELQPLSSKRSNTQVITQESLLAAQREDLLRV 60

Query: 61  MDLLSLREYHARTLLIHYRWDVEKLSAVFVEKGKSYLFATAGVTTIEQQSNSSSEP-SSM 120
           M+LLS++E+HARTLLIHY+WDVEKL AVFVEKGK  LF+ AGVT  + Q  +SS P SS 
Sbjct: 61  MELLSIKEHHARTLLIHYQWDVEKLFAVFVEKGKDSLFSGAGVTVFDYQYGNSSFPQSSQ 120

Query: 121 VMCDICMEEVHGNEATRMDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAV 180
           + CD+CME++ G+  TRMDCGHCFCNNCWTEHF V+INEGQS+RIRCMAH+CNAICDE +
Sbjct: 121 MSCDVCMEDLPGDHMTRMDCGHCFCNNCWTEHFTVQINEGQSKRIRCMAHQCNAICDEDI 180

Query: 181 VRNLVSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIRVEDDEFCEVECSCGL 240
           VR+LVSK+ PDLA KFDR+LLESYIEDN+ VKWCPSTPHCGNAIR EDD+ CEVECSCGL
Sbjct: 181 VRSLVSKKRPDLAAKFDRYLLESYIEDNRMVKWCPSTPHCGNAIRAEDDKLCEVECSCGL 240

Query: 241 QFCFSCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVS 300
           QFCFSCL +AHSPCSCLMWELW KKCRDESET+NWITVHTK CPKC+KPVEKNGGCNLV 
Sbjct: 241 QFCFSCLCQAHSPCSCLMWELWRKKCRDESETINWITVHTKLCPKCYKPVEKNGGCNLVR 300

Query: 301 CICGQAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAH 360
           CICGQ FCWLCGGATG +HT+ SI+GHSCGRY+++ E++ ERAKRDL RY HYH+RYKAH
Sbjct: 301 CICGQCFCWLCGGATGSDHTYRSIAGHSCGRYQDDKEKQMERAKRDLNRYTHYHHRYKAH 360

Query: 361 TDSFKLESKLKESIQEKISISEERESMLRDFSWVNNGLYRLFRSRRVLSYSYPFAFYMFG 420
           TDS KLE KL+++I EK+S SE+RE  L+DFSWV NGL RLFRSRRVLSYSY FA+YMFG
Sbjct: 361 TDSSKLEDKLRDTIHEKVSKSEKRELKLKDFSWVTNGLDRLFRSRRVLSYSYAFAYYMFG 420

Query: 421 DELFKDEMTQEEREIKQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDKVMEIRMQVINL 480
           +E+FKDEMT EEREIK++LFEDQQQQLE+NVEKLS+FLEEPFD+++ DKVM IR+Q+INL
Sbjct: 421 EEMFKDEMTPEEREIKKNLFEDQQQQLESNVEKLSQFLEEPFDEFSNDKVMAIRIQIINL 480

Query: 481 SVITDTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSASWNSRANTTDKYLP 540
           SV  DTLCKKMY+CIENDLLGSL+LGIHNI+PY+SKGIE+A +  ASWNS+    DK+ P
Sbjct: 481 SVAVDTLCKKMYECIENDLLGSLQLGIHNISPYRSKGIEQAAQFYASWNSK--DADKFQP 540

Query: 541 SDCGTSGGLSERDRSTGLNSEENG--CSSRKRARAEGVAGN----FFDLNLPAGVVDRN 590
            D GTSG  S  ++++G  S E+    SS+KR + EG   N      DLNLPA  VD+N
Sbjct: 541 LDSGTSGVTSRPEQASGSRSSEDTICSSSQKRPKKEGSFLNNKVTLLDLNLPADFVDQN 597

BLAST of MC02g0966 vs. TAIR 10
Match: AT2G16090.1 (RING/U-box superfamily protein )

HSP 1 Score: 852.0 bits (2200), Expect = 2.7e-247
Identity = 427/597 (71.52%), Postives = 488/597 (81.74%), Query Frame = 0

Query: 1   MEDCFSSDED--YYDSDRESLHGLENEES-EIQWAPEGPATKVITKESLLAAQKEDMRRV 60
           M+D  S +E+  YY SD+ESL+G++N+ES  I  +      KVITKESLLAAQ+ED+RRV
Sbjct: 1   MDDNLSGEEEDYYYSSDQESLNGIDNDESVSIPVSSRSNTVKVITKESLLAAQREDLRRV 60

Query: 61  MDLLSLREYHARTLLIHYRWDVEKLSAVFVEKGKSYLFATAGVTTIEQQS--NSSSEPSS 120
           M+LLS++E+HARTLLIHYRWDVEKL AV VEKGK  LF+ AGVT +E QS  +S S  SS
Sbjct: 61  MELLSVKEHHARTLLIHYRWDVEKLFAVLVEKGKDSLFSGAGVTLLENQSCDSSVSGSSS 120

Query: 121 MVMCDICMEEVHGNEATRMDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEA 180
           M+ CDIC+E+V G + TRMDCGH FCNNCWT HF VKINEGQS+RI CMAHKCNAICDE 
Sbjct: 121 MMSCDICVEDVPGYQLTRMDCGHSFCNNCWTGHFTVKINEGQSKRIICMAHKCNAICDED 180

Query: 181 VVRNLVSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIRVEDDEFCEVECSCG 240
           VVR LVSK  PDLA KFDRFLLESYIEDNK VKWCPSTPHCGNAIRVEDDE CEVECSCG
Sbjct: 181 VVRALVSKSQPDLAEKFDRFLLESYIEDNKMVKWCPSTPHCGNAIRVEDDELCEVECSCG 240

Query: 241 LQFCFSCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLV 300
           LQFCFSC S+AHSPCSC+MWELW KKC DESETVNWITVHTKPCPKCHKPVEKNGGCNLV
Sbjct: 241 LQFCFSCSSQAHSPCSCVMWELWRKKCFDESETVNWITVHTKPCPKCHKPVEKNGGCNLV 300

Query: 301 SCICGQAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKA 360
           +C+C Q+FCWLCG ATGR+HTW+ ISGHSCGR++E+ E++ ERAKRDL RYMHYHNRYKA
Sbjct: 301 TCLCRQSFCWLCGEATGRDHTWARISGHSCGRFQEDKEKQMERAKRDLKRYMHYHNRYKA 360

Query: 361 HTDSFKLESKLKESIQEKISISEERESMLRDFSWVNNGLYRLFRSRRVLSYSYPFAFYMF 420
           H DS KLE+KL  +I +K+SISE+RE  L+DFSW  NGL+RLFRSRRVLSYSYPFAFYMF
Sbjct: 361 HIDSSKLEAKLSNNISKKVSISEKRELQLKDFSWATNGLHRLFRSRRVLSYSYPFAFYMF 420

Query: 421 GDELFKDEMTQEEREIKQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDKVMEIRMQVIN 480
           GDELFKDEM+ EEREIKQ+LFEDQQQQLEANVEKLSKFLEEPFDQ+  DKVM+IR+QVIN
Sbjct: 421 GDELFKDEMSSEEREIKQNLFEDQQQQLEANVEKLSKFLEEPFDQFADDKVMQIRIQVIN 480

Query: 481 LSVITDTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSASWNSRANTTDKYL 540
           LSV  DTLC+ MY+CIENDLLGSL+LGIHNI PY+S GIE+A +  +S NS+        
Sbjct: 481 LSVAVDTLCENMYECIENDLLGSLQLGIHNITPYRSNGIERASDFYSSQNSKEAVGQS-- 540

Query: 541 PSDCGTSGGLSERDRSTGLNSEENGCSSRKRARAEGVAGN----FFDLNLPAGVVDR 589
            SDCG +  L +   S    SE+  CSS KRAR +    N      DLNLPA  ++R
Sbjct: 541 -SDCGWTSRLDQALESG--KSEDTSCSSGKRARIDESYRNSQTTLLDLNLPAEAIER 592

BLAST of MC02g0966 vs. TAIR 10
Match: AT4G34370.2 (RING/U-box superfamily protein )

HSP 1 Score: 818.9 bits (2114), Expect = 2.6e-237
Identity = 409/599 (68.28%), Postives = 491/599 (81.97%), Query Frame = 0

Query: 1   MEDCFSSDED--YYDSDRESLHGLENEESEIQ-WAPEGPATKVITKESLLAAQKEDMRRV 60
           M+D FS++E+  YY SD++SL G++NEESE+Q  + +   T+VIT+ESLLAAQ+ED+ RV
Sbjct: 1   MDDYFSAEEEACYYSSDQDSLDGIDNEESELQPLSSKRSNTQVITQESLLAAQREDLLRV 60

Query: 61  MDLLSLREYHARTLLIHYRWDVEKLSAVFVEKGKSYLFATAGVTTIEQQSNSSSEP-SSM 120
           M+LLS++E+HARTLLIHY+WDVEKL AVFVEKGK  LF+ AGVT  + Q  +SS P SS 
Sbjct: 61  MELLSIKEHHARTLLIHYQWDVEKLFAVFVEKGKDSLFSGAGVTVFDYQYGNSSFPQSSQ 120

Query: 121 VMCDICMEEVHGNEATRMDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAV 180
           + CD+CME++ G                WTEHF V+INEGQS+RIRCMAH+CNAICDE +
Sbjct: 121 MSCDVCMEDLPG----------------WTEHFTVQINEGQSKRIRCMAHQCNAICDEDI 180

Query: 181 VRNLVSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIRVEDDEFCEVECSCGL 240
           VR+LVSK+ PDLA KFDR+LLESYIEDN+ VKWCPSTPHCGNAIR EDD+ CEVECSCGL
Sbjct: 181 VRSLVSKKRPDLAAKFDRYLLESYIEDNRMVKWCPSTPHCGNAIRAEDDKLCEVECSCGL 240

Query: 241 QFCFSCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVS 300
           QFCFSCL +AHSPCSCLMWELW KKCRDESET+NWITVHTK CPKC+KPVEKNGGCNLV 
Sbjct: 241 QFCFSCLCQAHSPCSCLMWELWRKKCRDESETINWITVHTKLCPKCYKPVEKNGGCNLVR 300

Query: 301 CICGQAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAH 360
           CICGQ FCWLCGGATG +HT+ SI+GHSCGRY+++ E++ ERAKRDL RY HYH+RYKAH
Sbjct: 301 CICGQCFCWLCGGATGSDHTYRSIAGHSCGRYQDDKEKQMERAKRDLNRYTHYHHRYKAH 360

Query: 361 TDSFKLESKLKESIQEKISISEERESMLRDFSWVNNGLYRLFRSRRVLSYSYPFAFYMFG 420
           TDS KLE KL+++I EK+S SE+RE  L+DFSWV NGL RLFRSRRVLSYSY FA+YMFG
Sbjct: 361 TDSSKLEDKLRDTIHEKVSKSEKRELKLKDFSWVTNGLDRLFRSRRVLSYSYAFAYYMFG 420

Query: 421 DELFKDEMTQEEREIKQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDKVMEIRMQVINL 480
           +E+FKDEMT EEREIK++LFEDQQQQLE+NVEKLS+FLEEPFD+++ DKVM IR+Q+INL
Sbjct: 421 EEMFKDEMTPEEREIKKNLFEDQQQQLESNVEKLSQFLEEPFDEFSNDKVMAIRIQIINL 480

Query: 481 SVITDTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSASWNSRANTTDKYLP 540
           SV  DTLCKKMY+CIENDLLGSL+LGIHNI+PY+SKGIE+A +  ASWNS+    DK+ P
Sbjct: 481 SVAVDTLCKKMYECIENDLLGSLQLGIHNISPYRSKGIEQAAQFYASWNSK--DADKFQP 540

Query: 541 SDCGTSGGLSERDRSTGLNSEENG--CSSRKRARAEGVAGN----FFDLNLPAGVVDRN 590
            D GTSG  S  ++++G  S E+    SS+KR + EG   N      DLNLPA  VD+N
Sbjct: 541 LDSGTSGVTSRPEQASGSRSSEDTICSSSQKRPKKEGSFLNNKVTLLDLNLPADFVDQN 581

BLAST of MC02g0966 vs. TAIR 10
Match: AT3G27710.1 (RING/U-box superfamily protein )

HSP 1 Score: 647.5 bits (1669), Expect = 1.0e-185
Identity = 326/551 (59.17%), Postives = 418/551 (75.86%), Query Frame = 0

Query: 2   EDCFSSDEDYYDSDRESLHGLEN-EESEIQWAP---EGPATKVITKESLLAAQKEDMRRV 61
           +D    D+DY + + E+    +N  E+E+   P       ++VI KESL+AAQKE + RV
Sbjct: 3   DDYMMLDDDYGEEEDENYSEDDNYSEAEVDLQPVTSTKSTSQVIKKESLVAAQKEILVRV 62

Query: 62  MDLLSLREYHARTLLIHYRWDVEKLSAVFVEKGKSYLFATAGVTTIEQQSNSSSEPSSMV 121
           M+LLS++E  ARTLLI+Y+W+VEKL +VF ++GK  +F+ AG+T       +S +    +
Sbjct: 63  MELLSVKENQARTLLIYYQWNVEKLFSVFADQGKDRMFSCAGLTVFVPSLVTSKK---TM 122

Query: 122 MCDICME-EVHGNEATRMDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAV 181
            CD+CME ++  N  TRM+CGH FCN+CW  HF VKINEG+S+RI CMAH+C AICDE V
Sbjct: 123 KCDVCMEDDLPSNVMTRMECGHRFCNDCWIGHFTVKINEGESKRILCMAHECKAICDEDV 182

Query: 182 VRNLVSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIRVEDD--EFCEVECSC 241
           VR LVS   P+LA+++DRFL+ESY+EDN  VKWCPS PHCG+AIR  +D  +  EV CSC
Sbjct: 183 VRKLVS---PELADRYDRFLIESYVEDNNMVKWCPSKPHCGSAIRKIEDGHDVVEVGCSC 242

Query: 242 GLQFCFSCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNL 301
           GLQFCFSCLSE+HSPCSCLMW+LW KKC DESETVNWITV+TK CPKC KP++K  GCNL
Sbjct: 243 GLQFCFSCLSESHSPCSCLMWKLWKKKCEDESETVNWITVNTKLCPKCSKPIQKRDGCNL 302

Query: 302 VSCICGQAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYK 361
           ++C CGQ FCWLCG ATGR+HT++SI+GHSCGRYK+E  ++ ERA+RDL RY HYH RYK
Sbjct: 303 MTCKCGQHFCWLCGQATGRDHTYTSIAGHSCGRYKDEKVRQLERAQRDLDRYTHYHYRYK 362

Query: 362 AHTDSFKLESKLKESIQEK-ISISEER-ESMLRDFSWVNNGLYRLFRSRRVLSYSYPFAF 421
           AH DS KLE KL++SI EK +S SE + + + +++SWV + + RLF SRR+LS SYPFAF
Sbjct: 363 AHIDSLKLEDKLRKSILEKAVSNSETKDQKVFKEYSWVTDAVNRLFISRRILSQSYPFAF 422

Query: 422 YMFGDELFKDEMTQEEREIKQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDKVMEIRMQ 481
           YMFG+ELFKDEM+++EREIK++LFEDQQQQLE NVEKLSK LEEPFD+Y  +KV+E+   
Sbjct: 423 YMFGEELFKDEMSEKEREIKKNLFEDQQQQLEGNVEKLSKILEEPFDEYDHEKVVEMMRH 482

Query: 482 VINLSVITDTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSASWNSRANTTD 541
           + NL+ + D LCK+MY+CIEN+LLG ++ G HNIAPY+SKGIE+A E  A          
Sbjct: 483 LTNLTAVVDNLCKEMYECIENELLGPIQFGNHNIAPYRSKGIEQATEFCAE--------- 535

Query: 542 KYLPSDCGTSG 544
               SDCG+SG
Sbjct: 543 ---SSDCGSSG 535

BLAST of MC02g0966 vs. TAIR 10
Match: AT3G27720.1 (IBR domain containing protein )

HSP 1 Score: 520.4 bits (1339), Expect = 1.9e-147
Identity = 271/485 (55.88%), Postives = 343/485 (70.72%), Query Frame = 0

Query: 43  KESLLAAQKEDMRRVMDLLSLREYHARTLLIHYRWDVEKLSAVFVEKGKSYLFATAGVTT 102
           +ES L   +E   +VM+ LS+ E  ARTLLI Y+W+V+KL +V+ ++GK  LF+ A    
Sbjct: 31  EESDLQHSREPTSQVMEFLSVTENQARTLLIQYQWNVDKLFSVYTDQGKDVLFSRA---- 90

Query: 103 IEQQSNSSSEPSSMVMCDICMEEVHGNEATRMDCGHCFCNNCWTEHFIVKINEGQSRRIR 162
            E++     E       +   EE  G E    + G     +   E  +    E +  +  
Sbjct: 91  -EEEEGEDKEEEEGGEDE---EEEEGGEDEEAEEGG---EDEEAEEGVEDEEEEEDEK-- 150

Query: 163 CMAHKCNAICDEAVVRNLVSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIR- 222
                     D+ V   LVS    +LA KFDRFL+ESY+EDN  VKWCPSTPHCGNAIR 
Sbjct: 151 ----------DDEV--QLVS---TELAEKFDRFLIESYVEDNNMVKWCPSTPHCGNAIRN 210

Query: 223 -VEDDEFCEVECSCGLQFCFSCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCP 282
             +D +  EVECSCGLQFCFSCLSE+HSPCSCLMW+LW KKC DESETVNW+TV+TK CP
Sbjct: 211 IKDDGDVDEVECSCGLQFCFSCLSESHSPCSCLMWKLWKKKCEDESETVNWMTVNTKLCP 270

Query: 283 KCHKPVEKNGGCNLVSCICGQAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAK 342
           KC KP++K  GCN ++C CGQ FCWLCG ATGR+H++SSI+GHSCGRYKEE  ++ ERA+
Sbjct: 271 KCSKPIQKRDGCNHMTCKCGQHFCWLCGQATGRDHSYSSIAGHSCGRYKEEKVRQLERAQ 330

Query: 343 RDLYRYMHYHNRYKAHTDSFKLESKLKESIQEKISISEER--ESMLRDFSWVNNGLYRLF 402
           RDL RY HYH RYKAH DS KLE KLK+SI +K  ++ E   + + +++SW+ + + RLF
Sbjct: 331 RDLDRYTHYHYRYKAHIDSLKLEDKLKKSILKKAVLNSETKDQKVFKEYSWIIDAVNRLF 390

Query: 403 RSRRVLSYSYPFAFYMFGDELFKDEMTQEEREIKQHLFEDQQQQLEANVEKLSKFLEEPF 462
           RSRR+LSYSYPF FYMFG ELFKD+M+ EER IK++LFEDQQQQLE NVE+LSK LEEPF
Sbjct: 391 RSRRILSYSYPFVFYMFGKELFKDDMSDEERNIKKNLFEDQQQQLEGNVERLSKILEEPF 450

Query: 463 DQYTKDKVMEIRMQVINLSVITDTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKAL 522
           D+Y  +KV+E+   + NL+ + D LCK+MY+CIEN+LLG L  GIHNIAPY+SKGIE+A 
Sbjct: 451 DEYDHEKVVEMMRHLTNLTAVVDNLCKEMYECIENELLGPLISGIHNIAPYRSKGIEQAA 487

Query: 523 ELSAS 524
           E SAS
Sbjct: 511 EFSAS 487

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q949V62.2e-24970.45Probable E3 ubiquitin-protein ligase ARI1 OS=Arabidopsis thaliana OX=3702 GN=ARI... [more]
Q84RR23.9e-24671.52Probable E3 ubiquitin-protein ligase ARI2 OS=Arabidopsis thaliana OX=3702 GN=ARI... [more]
Q9LVX01.4e-18459.17Probable E3 ubiquitin-protein ligase ARI3 OS=Arabidopsis thaliana OX=3702 GN=ARI... [more]
Q9LVW99.4e-18461.10Putative E3 ubiquitin-protein ligase ARI4 OS=Arabidopsis thaliana OX=3702 GN=ARI... [more]
Q949813.1e-6232.82E3 ubiquitin-protein ligase ariadne-1 OS=Drosophila melanogaster OX=7227 GN=ari-... [more]
Match NameE-valueIdentityDescription
XP_022148613.10.0100.00probable E3 ubiquitin-protein ligase ARI1 isoform X1 [Momordica charantia][more]
XP_022148614.10.099.32probable E3 ubiquitin-protein ligase ARI1 isoform X2 [Momordica charantia][more]
XP_038901258.10.094.40probable E3 ubiquitin-protein ligase ARI2 isoform X2 [Benincasa hispida][more]
XP_038901257.10.093.76probable E3 ubiquitin-protein ligase ARI2 isoform X1 [Benincasa hispida][more]
XP_004153333.10.094.23probable E3 ubiquitin-protein ligase ARI2 isoform X1 [Cucumis sativus] >KGN61769... [more]
Match NameE-valueIdentityDescription
A0A6J1D4K10.0100.00probable E3 ubiquitin-protein ligase ARI1 isoform X1 OS=Momordica charantia OX=3... [more]
A0A6J1D4I20.099.32probable E3 ubiquitin-protein ligase ARI1 isoform X2 OS=Momordica charantia OX=3... [more]
A0A0A0LL320.094.23Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G238850 PE=3 SV=1[more]
A0A5D3CP890.094.23Putative E3 ubiquitin-protein ligase ARI2 OS=Cucumis melo var. makuwa OX=1194695... [more]
A0A1S3C9A10.094.23probable E3 ubiquitin-protein ligase ARI2 OS=Cucumis melo OX=3656 GN=LOC10349843... [more]
Match NameE-valueIdentityDescription
AT4G34370.11.6e-25070.45RING/U-box superfamily protein [more]
AT2G16090.12.7e-24771.52RING/U-box superfamily protein [more]
AT4G34370.22.6e-23768.28RING/U-box superfamily protein [more]
AT3G27710.11.0e-18559.17RING/U-box superfamily protein [more]
AT3G27720.11.9e-14755.88IBR domain containing protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002867IBR domainSMARTSM00647ibrneu5coord: 190..252
e-value: 3.7E-21
score: 86.3
coord: 260..325
e-value: 0.012
score: 23.3
IPR002867IBR domainPFAMPF01485IBRcoord: 191..252
e-value: 1.4E-12
score: 47.6
coord: 268..310
e-value: 1.1E-5
score: 25.6
NoneNo IPR availableGENE3D1.20.120.1750coord: 248..496
e-value: 7.7E-64
score: 217.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 544..560
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..27
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 535..564
NoneNo IPR availablePANTHERPTHR11685:SF345RBR-TYPE E3 UBIQUITIN TRANSFERASEcoord: 6..516
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 260..325
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 191..259
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 114..202
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 113..204
e-value: 6.0E-19
score: 69.8
IPR031127E3 ubiquitin ligase RBR familyPANTHERPTHR11685RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAININGcoord: 6..516
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 119..167
score: 9.74522
IPR044066TRIAD supradomainPROSITEPS51873TRIADcoord: 115..329
score: 45.558868

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC02g0966.1MC02g0966.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032436 positive regulation of proteasomal ubiquitin-dependent protein catabolic process
biological_process GO:0000209 protein polyubiquitination
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0005737 cytoplasm
cellular_component GO:0000151 ubiquitin ligase complex
molecular_function GO:0046872 metal ion binding
molecular_function GO:0031624 ubiquitin conjugating enzyme binding
molecular_function GO:0061630 ubiquitin protein ligase activity
molecular_function GO:0004842 ubiquitin-protein transferase activity