MC02g0747 (gene) Bitter gourd (Dali-11) v1

BLAST of MC02g0747 vs. ExPASy Swiss-Prot
Match: Q9STF2 (Protein PLASTID TRANSCRIPTIONALLY ACTIVE 16, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PTAC16 PE=1 SV=1)

HSP 1 Score: 500.0 bits (1286), Expect = 3.6e-140
Identity = 316/576 (54.86%), Postives = 403/576 (69.97%), Query Frame = 0

Query: 6   TSNSFILTTAPNTRRSFKSHRRQFAVYARRSGTFGSLRLGKSSNGSPSSDDAPPEESGNS 65
           +S SF LTTAP     F   + +  V+A+++    + +LGK+       DD+  ++ G  
Sbjct: 4   SSTSFPLTTAPPQGVRFNRRKPRLTVWAKQT----AFQLGKTK----GDDDSEGKQKG-K 63

Query: 66  NPFRFNFGKVPDMKSLIPVVSNPSSSGLSFGNARRKDPNTVFVAGATGQAGIRLAQTLLR 125
           NPF+F+FGK+PDMKSLIPVV+NP S+GL FGN R+KDP T+FVAGATGQAGIR+AQTLL+
Sbjct: 64  NPFQFDFGKLPDMKSLIPVVTNP-STGLVFGNNRKKDPGTIFVAGATGQAGIRIAQTLLQ 123

Query: 126 EGFSVRAGVPELDAAQELARLAAKYMVISNEESKRLNAVESTFQDAESIAKAIGNAGKVV 185
            GFSVRAGVP+L AAQ+LAR+AA Y ++SN+E KRLNAV+S FQDAESIAKAIGNA KVV
Sbjct: 124 RGFSVRAGVPDLGAAQDLARVAATYKILSNDEVKRLNAVQSPFQDAESIAKAIGNATKVV 183

Query: 186 VTIGAAENGPTSEVTTSDALQVIQAAQLAGVSHVAIVYDGSALNSSTYNVLDGLSSFFNN 245
           VT+GA ENGP ++V+TSDAL V+QAA+LAGVSHVAIVYDG+ ++ STYNVLDG++SFF N
Sbjct: 184 VTVGATENGPDAQVSTSDALLVVQAAELAGVSHVAIVYDGT-ISGSTYNVLDGITSFFGN 243

Query: 246 LFSRSQQLSVSELLQKIVETDVGYTFIKTNLVEDFAPERTYNVVVGAE------GSASSN 305
           LF++SQ L++S+L++K+ +TDV YT IKT+L EDF+PE+ YNVVV AE      GS+SS 
Sbjct: 244 LFAKSQPLTISDLIEKVAQTDVAYTLIKTSLTEDFSPEKAYNVVVSAEGSNSGSGSSSSE 303

Query: 306 DYKVAKSQIASLVAGVFSNTAVAENKVVEVYSSPSAPSSSVEQLFSAIPKDGRREAYAEA 365
            YKV K +IASLVA +F+NTAVAENKVVEV + PSAPS  V++LFS IP+DGRR+ YA+A
Sbjct: 304 AYKVPKLKIASLVADIFANTAVAENKVVEVSTDPSAPSRPVDELFSVIPEDGRRKVYADA 363

Query: 366 QVKAKAEEEAIRAAEKARQEAEAAKKKEEQVAKRLSEKAAKASSSPPAAAAAEESDQIAF 425
             + +AEEEA  AA+KAR+ AEAAK+ E+Q                              
Sbjct: 364 IARERAEEEAKVAADKAREAAEAAKEFEKQ------------------------------ 423

Query: 426 FNTFLNKAKDFSSEQSQKLKKLSEKEPQAPSKAK-AEEEAESAGSFVNNFFSKAKDLGSA 485
                             ++KLSEKE +A S A+ A+++A++ G  V+  F+KAKD+ S 
Sbjct: 424 ------------------MQKLSEKEAEAASLAEDAQQKADAVGVTVDGLFNKAKDISSG 483

Query: 486 QPWEKLAFQLQ---KPPSEESNVQVATVRGQAKARALPSKKAAVRQPQTNSNSKPPFAVK 545
             W KL  Q     +  SE   VQVATVRGQAKAR LP KKA V+Q  ++     PFA K
Sbjct: 484 LSWNKLGSQFATAIQNASETPKVQVATVRGQAKARNLPPKKAVVKQRPSS-----PFASK 510

Query: 546 AKENSKPKGKEEGSKAEVRKVFGGLFSQETIYVDDD 572
            KE  +PK  E+    EVRKVFGGLF QETIY+DDD
Sbjct: 544 PKE-ERPKKPEK----EVRKVFGGLFKQETIYIDDD 510

BLAST of MC02g0747 vs. ExPASy Swiss-Prot
Match: Q8H0U5 (Protein TIC 62, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIC62 PE=1 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 7.3e-08
Identity = 83/339 (24.48%), Postives = 158/339 (46.61%), Query Frame = 0

Query: 97  NARRKDPNTVFVAGATGQAGIRLAQTLLREGFSVRAGVPELDAAQELARLAAKYMVISNE 156
           N   K+ + VFVAGATG+ G R  + LL+ GF VRAGV     A  L + + K M + N 
Sbjct: 75  NLNSKEEDLVFVAGATGKVGSRTVRELLKLGFRVRAGVRSAQRAGSLVQ-SVKEMKLQNT 134

Query: 157 ES-----KRLNAVESTFQDAESIAKAIGNAGKVVVTIGAAENGPTSEVTTSDAL------ 216
           +      ++L  VE   +  +SI  A+GNA  ++  IGA+E    S++T    +      
Sbjct: 135 DEGTQPVEKLEIVECDLEKKDSIQPALGNASVIICCIGASEK-EISDITGPYRIDYLATK 194

Query: 217 QVIQAAQLAGVSHVAIVYDGSALNSSTYNVLDGLSSFFNNLFSRSQQLSVSELLQKIVET 276
            ++ AA  A V++  +V   ++L ++ +     + + F  +    ++       + ++E+
Sbjct: 195 NLVDAATSAKVNNFILV---TSLGTNKFGFPAAILNLFWGVLCWKRKAE-----EALIES 254

Query: 277 DVGYTFIKTNLVE--DFAPERTYNVVVGAEGSASSNDYKVAKSQIASLVAGVFSNTAVAE 336
            + Y  ++   +E    A + T+N+ +  + +      +V+  Q+A L+A +  N  ++ 
Sbjct: 255 GLNYAIVRPGGMERPTDAYKETHNLTLALDDTLFGG--QVSNLQVAELLACMAKNPQLSF 314

Query: 337 NKVVEVYSSPSAPSSSVEQLFSAIPKDGRREAYAEAQVKAK----AEEEAIRAAEKARQE 396
           +K+VEV +  +AP + +E+L   IP         +A V  K       + +     A +E
Sbjct: 315 SKIVEVVAETTAPLTPIEKLLEKIPSKRPYVPPPKASVATKEVKPVPTKPVTQEPTAPKE 374

Query: 397 AEAAKKKEEQVAKRLSEKAAKASSSPPAAAAAEESDQIA 419
            EA  K++    + LS  A+     PP +     +  ++
Sbjct: 375 DEAPPKEKNVKPRPLSPYASYEDLKPPTSPIPNSTTSVS 401

BLAST of MC02g0747 vs. ExPASy Swiss-Prot
Match: Q10A77 (Protein TIC 62, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=TIC62 PE=1 SV=1)

HSP 1 Score: 58.2 bits (139), Expect = 3.6e-07
Identity = 91/320 (28.44%), Postives = 140/320 (43.75%), Query Frame = 0

Query: 101 KDPNTVFVAGATGQAGIRLAQTLLREGFSVRAGVPELDAAQELARLAAKYMVISNEES-- 160
           K+ + VF+AGATG+ G R  +  ++ GF VRAGV     A  L +   +  V  +  S  
Sbjct: 70  KENDLVFIAGATGKVGSRAVREFIKLGFRVRAGVRSAQRASSLVQSVEQLKVDDDATSPA 129

Query: 161 KRLNAVESTF--QDAESIAKAIGNAGKVVVTIGAAE------NGPTSEVTTSDALQVIQA 220
           +RL  VE     Q    I  AIGNA  VV +IGA+E       GP   +       ++QA
Sbjct: 130 ERLEIVECDLEKQAQSDIVSAIGNAAIVVCSIGASEKDILDVTGP-YRIDYMATNNLVQA 189

Query: 221 AQLAGVSHVAIVYDGSALNSSTYNVLDGLSSFFNNLFSRSQQLSVSELLQKIVETDVGYT 280
           A  A V H  +V   ++L ++      G  +F  NLF            + ++ + + YT
Sbjct: 190 ATAAKVEHFILV---TSLGTNRI----GFPAFLLNLF-WGVLCWKRRAEEALIGSGLPYT 249

Query: 281 FIKTNLVE--DFAPERTYNVVVGAEGSASSNDYKVAKSQIASLVAGVFSNTAVAENKVVE 340
            ++   +E    A + T+N+VV  E +       V+  Q+A L+A + SN   A  KVVE
Sbjct: 250 IVRPGGMERPTDAFKETHNLVVAVEDTYVGG--LVSNLQVAELIACIASNRRTAYCKVVE 309

Query: 341 VYSSPSAPSSSVEQLFSAIPKDGRREAYAEAQVKAKAEEEAIRAAEKARQEAEAAKKKEE 400
             +  +AP    E   + IP   +R+   E +V                Q+ E   K  +
Sbjct: 310 AIAETTAPLLPTEDQLANIP--SKRQPPPEPEVV---------------QQGETPPKPIQ 361

Query: 401 QVAKRLSEKAAKASSSPPAA 409
           Q  + LS   A     PP++
Sbjct: 370 QSQRPLSPYTAFVDLKPPSS 361

BLAST of MC02g0747 vs. ExPASy Swiss-Prot
Match: Q8SKU2 (Protein TIC 62, chloroplastic OS=Pisum sativum OX=3888 GN=TIC62 PE=1 SV=2)

HSP 1 Score: 53.9 bits (128), Expect = 6.9e-06
Identity = 95/379 (25.07%), Postives = 168/379 (44.33%), Query Frame = 0

Query: 101 KDPNTVFVAGATGQAGIRLAQTLLREGFSVRAGVPELDAA----QELARLAAKYMVISNE 160
           KD N VFVAGATG+ G R  + L++ GF VRAGV     A    Q + +L         E
Sbjct: 86  KDDNLVFVAGATGKVGSRTVRELIKLGFKVRAGVRNAQKAGALVQSVKQLKLDGASGGGE 145

Query: 161 ESKRLNAVESTFQDAESIAKAIGNAGKVVVTIGAAE------NGPTSEVTTSDALQVIQA 220
             ++L  VE   + A+ I  A+GNA  V+  IGA+E       GP   +       ++ A
Sbjct: 146 AVEKLEIVECDLEKADQIGSALGNASTVICAIGASEKEIFDITGP-CRIDYRATKNLVDA 205

Query: 221 AQLAGVSHVAIVYDGSALNSSTYNVLDGLSSFFNNLFSRSQQLSVSELLQKIVETDVGYT 280
           A +A V+H  +V   ++L ++ +    GL +   NLF     +   +  + ++ + + YT
Sbjct: 206 ATVAKVNHFILV---TSLGTNKF----GLPAAILNLF-WGVLIWKRKAEEALLASGIPYT 265

Query: 281 FIKTNLVE--DFAPERTYNVVVGAEGSASSNDYKVAKSQIASLVAGVFSNTAVAENKVVE 340
            ++   +E    A + T+NV +  E +      +V+  Q+A L+A +  N  ++  K+VE
Sbjct: 266 IVRPGGMERPTDAYKETHNVTLSTEDTLFGG--QVSNLQVAELMAIMAKNPDLSYCKIVE 325

Query: 341 VYSSPSAPSSSVEQLFSAIPKDGRREAYAEAQVKAKAEEEA-----------IRAAEKAR 400
           V +  +AP +  E+L + IP    +  Y  +  K +  + A           +       
Sbjct: 326 VIAETTAPLTPAEKLLTRIPS---QRPYIPSPKKVQKADTATVSNTGPSANVVAEVPSIA 385

Query: 401 QEAEAAKKKEEQVAKRLSEKAAKASSSPPAAAAAEESDQIAFFNTFLNKAKDFSSEQSQK 457
            + E A K   +  + LS   A     PP++ +  +  +    N         SS+    
Sbjct: 386 PQKETASKPVAKTEQPLSPYTAYDDLKPPSSPSPTKPSEKKQINISDAVPTPISSDTPSS 445

BLAST of MC02g0747 vs. NCBI nr
Match: XP_022148339.1 (protein plastid transcriptionally active 16, chloroplastic [Momordica charantia])

HSP 1 Score: 1023 bits (2645), Expect = 0.0
Identity = 566/571 (99.12%), Postives = 568/571 (99.47%), Query Frame = 0

Query: 1   MAAIITSNSFILTTAPNTRRSFKSHRRQFAVYARRSGTFGSLRLGKSSNGSPSSDDAPPE 60
           MAAIITSNSFILTTAPNTRRSFKSHRRQFAVYARRSGTFGSLRLGKSSNGSPSSDDAPPE
Sbjct: 1   MAAIITSNSFILTTAPNTRRSFKSHRRQFAVYARRSGTFGSLRLGKSSNGSPSSDDAPPE 60

Query: 61  ESGNSNPFRFNFGKVPDMKSLIPVVSNPSSSGLSFGNARRKDPNTVFVAGATGQAGIRLA 120
           ESGNSNPFRFNFGKVPDMKSLIPVVSNPSSSGLSFGNARRKDPNTVFVAGATGQAGIRLA
Sbjct: 61  ESGNSNPFRFNFGKVPDMKSLIPVVSNPSSSGLSFGNARRKDPNTVFVAGATGQAGIRLA 120

Query: 121 QTLLREGFSVRAGVPELDAAQELARLAAKYMVISNEESKRLNAVESTFQDAESIAKAIGN 180
           QTLLREGFSVRAGVPELDAAQELARLAAKYMVISNEESKRLNAVESTFQDAESIAKAIGN
Sbjct: 121 QTLLREGFSVRAGVPELDAAQELARLAAKYMVISNEESKRLNAVESTFQDAESIAKAIGN 180

Query: 181 AGKVVVTIGAAENGPTSEVTTSDALQVIQAAQLAGVSHVAIVYDGSALNSSTYNVLDGLS 240
           AGKVVVTIGAAENGPTSEVTTSDALQVIQAAQLAGVSHVAIVYDGSALNSSTYNVLDGLS
Sbjct: 181 AGKVVVTIGAAENGPTSEVTTSDALQVIQAAQLAGVSHVAIVYDGSALNSSTYNVLDGLS 240

Query: 241 SFFNNLFSRSQQLSVSELLQKIVETDVGYTFIKTNLVEDFAPERTYNVVVGAEGSASSND 300
           SFFNNLFSRSQQLSVSELLQKIVETDVGYTFIKTNLVEDFAPERTYNVVVGAEGSASSND
Sbjct: 241 SFFNNLFSRSQQLSVSELLQKIVETDVGYTFIKTNLVEDFAPERTYNVVVGAEGSASSND 300

Query: 301 YKVAKSQIASLVAGVFSNTAVAENKVVEVYSSPSAPSSSVEQLFSAIPKDGRREAYAEAQ 360
           YKVAKSQIASLVAGVFSNTAVAENKVVEVYSSPSAPSSSVEQLFSAIPKDGRREAYAEAQ
Sbjct: 301 YKVAKSQIASLVAGVFSNTAVAENKVVEVYSSPSAPSSSVEQLFSAIPKDGRREAYAEAQ 360

Query: 361 VKAKAEEEAIRAAEKARQEAEAAKKKEEQVAKRLSEKAAKASSSPPAAAAAEESDQIAFF 420
            KAKAEEEAIRAAEKARQEAEAAKKKEEQVAKRLSEKAAKASS PPAAAA EESDQIAFF
Sbjct: 361 AKAKAEEEAIRAAEKARQEAEAAKKKEEQVAKRLSEKAAKASSPPPAAAA-EESDQIAFF 420

Query: 421 NTFLNKAKDFSSEQSQKLKKLSEKEPQAPSKAKAEEEAESAGSFVNNFFSKAKDLGSAQP 480
           NTFLNKAKDFSSEQSQKLKKL+EKEPQAPSKAKAEEEAESAGSFVNNFFSKAKDLGSAQP
Sbjct: 421 NTFLNKAKDFSSEQSQKLKKLTEKEPQAPSKAKAEEEAESAGSFVNNFFSKAKDLGSAQP 480

Query: 481 WEKLAFQLQKPPSEESNVQVATVRGQAKARALPSKKAAVRQPQTNSNSKPPFAVKAKENS 540
           WEKLAFQLQKPPSEESNVQVATVRGQAKARALPSKKAAVRQPQTNSNSKPPFAVKAKENS
Sbjct: 481 WEKLAFQLQKPPSEESNVQVATVRGQAKARALPSKKAAVRQPQTNSNSKPPFAVKAKENS 540

Query: 541 KPKGKEEGSKAEVRKVFGGLFSQETIYVDDD 571
           KPKGKEEGSKAEVRKVFGGLFSQET+YVDDD
Sbjct: 541 KPKGKEEGSKAEVRKVFGGLFSQETLYVDDD 570

BLAST of MC02g0747 vs. NCBI nr
Match: XP_038901316.1 (protein PLASTID TRANSCRIPTIONALLY ACTIVE 16, chloroplastic [Benincasa hispida])

HSP 1 Score: 832 bits (2150), Expect = 4.03e-299
Identity = 484/579 (83.59%), Postives = 515/579 (88.95%), Query Frame = 0

Query: 1   MAAIITSNSFILTTAPNTRRSFKSHRRQFAVYARRSGTFGSLRLGKSSNGSPSSDDAPPE 60
           MAAII SNSFILTTAPN RRSFKSHRRQFAVYA+ SGTF S RLGKSSN SPSSDDAP E
Sbjct: 1   MAAIINSNSFILTTAPNARRSFKSHRRQFAVYAKSSGTFSSFRLGKSSNESPSSDDAPAE 60

Query: 61  ESGNSNPFRFNFGKVPDMKSLIPVVSNPSSSGLSFGNARRKDPNTVFVAGATGQAGIRLA 120
           +S NSNPFRF FGKVPD+KSLIPVV  PSS GLSFGNARRKD NTVFVAGATGQAGIRLA
Sbjct: 61  DSANSNPFRFAFGKVPDVKSLIPVVGEPSS-GLSFGNARRKDSNTVFVAGATGQAGIRLA 120

Query: 121 QTLLREGFSVRAGVPELDAAQELARLAAKYMVISNEESKRLNAVESTFQDAESIAKAIGN 180
           QTLLREGFSVRAGVPEL AAQELARLAAKY VISNEESKRLNAVES+FQDAE+IAKAIGN
Sbjct: 121 QTLLREGFSVRAGVPELGAAQELARLAAKYKVISNEESKRLNAVESSFQDAEAIAKAIGN 180

Query: 181 AGKVVVTIGAAENGPTSEVTTSDALQVIQAAQLAGVSHVAIVYDGSALNSSTYNVLDGLS 240
           A KVVVTIGA ENGPTSEVTTSDALQVIQAAQLAGVSHVAIVYDG+A +SSTYNVLDGLS
Sbjct: 181 ASKVVVTIGAGENGPTSEVTTSDALQVIQAAQLAGVSHVAIVYDGNASSSSTYNVLDGLS 240

Query: 241 SFFNNLFSRSQQLSVSELLQKIVETDVGYTFIKTNLVEDFAPERTYNVVVGAEGSASSND 300
           SFFNNLFSRSQ LSV ELLQKI ETDVGYTFIKTNLVEDFAPER YNVVVGAEGS SSND
Sbjct: 241 SFFNNLFSRSQPLSVVELLQKIAETDVGYTFIKTNLVEDFAPERAYNVVVGAEGSVSSND 300

Query: 301 YKVAKSQIASLVAGVFSNTAVAENKVVEVYSSPSAPSSSVEQLFSAIPKDGRREAYAEAQ 360
           YKVAKSQIASLVAGVFSNTAVAENKVVEVYSSPSAPSSSV+QLFSAIP DGRR+AYAEAQ
Sbjct: 301 YKVAKSQIASLVAGVFSNTAVAENKVVEVYSSPSAPSSSVDQLFSAIPTDGRRQAYAEAQ 360

Query: 361 VKAKAEEEAIRAAEKARQEAEAAKKKEEQVAK--RLSEK-AAKASSSPPAAAAAE----E 420
            KAKAEEEAIR AEKAR+EAEAAKK+E + AK  RLSEK AA+AS S  ++   E    E
Sbjct: 361 AKAKAEEEAIRTAEKAREEAEAAKKQEVEAAKAKRLSEKVAAQASPSSSSSLTEESQKQE 420

Query: 421 SDQIAFFNTFLNKAKDFSSEQSQKLKKLSEKEPQAPSKAKAEEEAESAGSFVNNFFSKAK 480
           SDQIAFFN+FLNKAKDFSSEQSQKLKKLSEKEP+ P+K +A E+A++  + VN+FF+KAK
Sbjct: 421 SDQIAFFNSFLNKAKDFSSEQSQKLKKLSEKEPR-PAKEEAPEKADNGANLVNDFFNKAK 480

Query: 481 DLGSAQPWEKLAFQLQKPPSEESNVQVATVRGQAKARALPSKKAAVRQPQTNSNSKPPFA 540
             GSAQPWEKLAFQL K  SEESN Q+ATVRGQAKARALP KKA+VRQ Q  +NSKP FA
Sbjct: 481 GFGSAQPWEKLAFQLPKA-SEESNAQIATVRGQAKARALPPKKASVRQTQ-KTNSKPSFA 540

Query: 541 VKAKENSKPKGK-EEGSKAEVRKVFGGLFSQETIYVDDD 571
           +K KENSKPK   +E +KAEVRKVFGGLF QETIYVDDD
Sbjct: 541 LKQKENSKPKAAAKEETKAEVRKVFGGLFKQETIYVDDD 575

BLAST of MC02g0747 vs. NCBI nr
Match: XP_023532201.1 (protein plastid transcriptionally active 16, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 828 bits (2140), Expect = 1.24e-297
Identity = 480/580 (82.76%), Postives = 516/580 (88.97%), Query Frame = 0

Query: 1   MAAIITSNSFILTTAPNTRRSFKSH-RRQFAVYARRSGTFGSLRLGKSSNGSPSSDDAPP 60
           MAAII SNSFILTTAPN RRSF ++ RRQF+VYA+ SGTF S RLGKSSNGSPSSD AP 
Sbjct: 1   MAAIINSNSFILTTAPNARRSFDNNSRRQFSVYAKSSGTFASFRLGKSSNGSPSSDGAPA 60

Query: 61  EESGNSNPFRFNFGKVPDMKSLIPVVSNPSSSGLSFGNARRKDPNTVFVAGATGQAGIRL 120
           E+SGNSNPFRFNFGK+PD+KSLIPVVS+ +SSGLSFGNARRKDPNTVFVAGA+GQAGIRL
Sbjct: 61  EDSGNSNPFRFNFGKIPDVKSLIPVVSD-TSSGLSFGNARRKDPNTVFVAGASGQAGIRL 120

Query: 121 AQTLLREGFSVRAGVPELDAAQELARLAAKYMVISNEESKRLNAVESTFQDAESIAKAIG 180
           AQTLLREGFSVRAGVPELDAAQELARLAAKY VISNEESKRLNAVES+FQDAESIAKAIG
Sbjct: 121 AQTLLREGFSVRAGVPELDAAQELARLAAKYKVISNEESKRLNAVESSFQDAESIAKAIG 180

Query: 181 NAGKVVVTIGAAENGPTSEVTTSDALQVIQAAQLAGVSHVAIVYDGSALNSSTYNVLDGL 240
           NA KVVVTIGA ENGPTSEVTTSDALQVIQAAQLAGV HVAIVYDG+A  SST NVLDGL
Sbjct: 181 NASKVVVTIGAGENGPTSEVTTSDALQVIQAAQLAGVGHVAIVYDGNASKSSTNNVLDGL 240

Query: 241 SSFFNNLFSRSQQLSVSELLQKIVETDVGYTFIKTNLVEDFAPERTYNVVVGAEGSASSN 300
           SSFFNNLFSRSQ+LSV ELLQKIVETDV YTFIK NL EDF PER YNVVVGAEGSASSN
Sbjct: 241 SSFFNNLFSRSQELSVVELLQKIVETDVSYTFIKANLAEDFEPERAYNVVVGAEGSASSN 300

Query: 301 DYKVAKSQIASLVAGVFSNTAVAENKVVEVYSSPSAPSSSVEQLFSAIPKDGRREAYAEA 360
           DYKVAKS+IASLVAGVFSNTAVAENKVVEVYS+PSAPSSSVEQLFSAIP DGRR+AYAEA
Sbjct: 301 DYKVAKSRIASLVAGVFSNTAVAENKVVEVYSTPSAPSSSVEQLFSAIPTDGRRQAYAEA 360

Query: 361 QVKAKAEEEAIRAAEKARQEAEAAKKKEEQVAKRLSEKAAKASSSPPAAAAAE----ESD 420
           Q KA+AEEEAIR AEKAR+EAEAAKK++ + AK  +EK A+A+++  AA + E    ESD
Sbjct: 361 QAKAQAEEEAIRTAEKAREEAEAAKKQQLEAAK--AEKTAQAAAAAAAAVSEESQKQESD 420

Query: 421 QIAFFNTFLNKAKDFSSEQSQKLKKLSEKEPQAPSKAKAEEEAE----SAGSFVNNFFSK 480
           ++AFFNT LNKAKDFSSEQSQKLKKLSEKEP+AP+K KAEE  E    +AGS VNNF SK
Sbjct: 421 EVAFFNTLLNKAKDFSSEQSQKLKKLSEKEPKAPTKPKAEEAPEKADSAAGSLVNNFLSK 480

Query: 481 AKDLGSAQPWEKLAFQLQKPPSEESNVQVATVRGQAKARALPSKKAAVRQPQTNSNSKPP 540
           AK  GS QPWEKLAFQL K  SEESNVQ+ATVRGQAKAR+LPSKKAAVRQ  TNS  KP 
Sbjct: 481 AKGFGSQQPWEKLAFQLPKL-SEESNVQIATVRGQAKARSLPSKKAAVRQAMTNS--KPS 540

Query: 541 FAVKAKENSKPKGKEEGSKAEVRKVFGGLFSQETIYVDDD 571
           FA K+KENSKPK KEE +KAEVRKVFGGLF QETIYVDDD
Sbjct: 541 FAAKSKENSKPKAKEE-AKAEVRKVFGGLFKQETIYVDDD 573

BLAST of MC02g0747 vs. NCBI nr
Match: KAG7035386.1 (Protein plastid transcriptionally active 16, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 827 bits (2135), Expect = 6.17e-297
Identity = 475/576 (82.47%), Postives = 513/576 (89.06%), Query Frame = 0

Query: 1   MAAIITSNSFILTTAPNTRRSFKSH-RRQFAVYARRSGTFGSLRLGKSSNGSPSSDDAPP 60
           MAAII SNSFILTTAPN RRSF ++ RRQF+VYA+ SGTF S RLGKSSNGSPSSD AP 
Sbjct: 1   MAAIINSNSFILTTAPNARRSFDNNSRRQFSVYAKSSGTFASFRLGKSSNGSPSSDGAPA 60

Query: 61  EESGNSNPFRFNFGKVPDMKSLIPVVSNPSSSGLSFGNARRKDPNTVFVAGATGQAGIRL 120
           E+SGNSNPFRF+FGK+PD+KSLIPVVS+ +SSGLSFGNAR KDPNTVFVAGA+GQAGIRL
Sbjct: 61  EDSGNSNPFRFDFGKIPDVKSLIPVVSD-TSSGLSFGNARGKDPNTVFVAGASGQAGIRL 120

Query: 121 AQTLLREGFSVRAGVPELDAAQELARLAAKYMVISNEESKRLNAVESTFQDAESIAKAIG 180
           AQTLLREGFSVRAGVPELDAAQELARLAAKY VISNEESKRLNAVES+FQDAESIAKAIG
Sbjct: 121 AQTLLREGFSVRAGVPELDAAQELARLAAKYKVISNEESKRLNAVESSFQDAESIAKAIG 180

Query: 181 NAGKVVVTIGAAENGPTSEVTTSDALQVIQAAQLAGVSHVAIVYDGSALNSSTYNVLDGL 240
           NA KVVVTIGA ENGPTSEVTTSDALQVIQAAQLAGV HVAIVYDG+A NSST NVLDGL
Sbjct: 181 NASKVVVTIGAGENGPTSEVTTSDALQVIQAAQLAGVGHVAIVYDGNASNSSTNNVLDGL 240

Query: 241 SSFFNNLFSRSQQLSVSELLQKIVETDVGYTFIKTNLVEDFAPERTYNVVVGAEGSASSN 300
           SSFFNNLFSRSQ LSV ELLQKIVETDV YTFIK NL EDF PER YNVVVGAEGSASSN
Sbjct: 241 SSFFNNLFSRSQALSVVELLQKIVETDVSYTFIKANLAEDFEPERAYNVVVGAEGSASSN 300

Query: 301 DYKVAKSQIASLVAGVFSNTAVAENKVVEVYSSPSAPSSSVEQLFSAIPKDGRREAYAEA 360
           DYKVAKS+IASLVAGVFSNTAVAENKVVEVYS+PSAPSSSVEQLFSAIP DGRR+AYAEA
Sbjct: 301 DYKVAKSRIASLVAGVFSNTAVAENKVVEVYSTPSAPSSSVEQLFSAIPTDGRRQAYAEA 360

Query: 361 QVKAKAEEEAIRAAEKARQEAEAAKKKEEQVAKRLSEKAAKASSSPPAAAAAEESDQIAF 420
           Q KAKAEEEAIR AEKAR+EAEAAKK++ + AK  +EK A+A+++    +  +ESD++AF
Sbjct: 361 QAKAKAEEEAIRTAEKAREEAEAAKKQQLEAAK--AEKTAQAAAAVAEESQKQESDEVAF 420

Query: 421 FNTFLNKAKDFSSEQSQKLKKLSEKEPQAPSKAKAEEEAE----SAGSFVNNFFSKAKDL 480
           FNT LNKAKDFSSEQSQKLKKLSEKEP+AP+K KAEE  E    +AGS VNNF SKAK  
Sbjct: 421 FNTLLNKAKDFSSEQSQKLKKLSEKEPKAPTKPKAEEAPEKADSAAGSLVNNFLSKAKGF 480

Query: 481 GSAQPWEKLAFQLQKPPSEESNVQVATVRGQAKARALPSKKAAVRQPQTNSNSKPPFAVK 540
           GS QPW+KLAFQ+ K P EESNVQ+ATVRGQAKAR+LPSKKAAVRQ  TNS  KP FA K
Sbjct: 481 GSQQPWDKLAFQIPKQP-EESNVQIATVRGQAKARSLPSKKAAVRQAMTNS--KPSFAAK 540

Query: 541 AKENSKPKGKEEGSKAEVRKVFGGLFSQETIYVDDD 571
           +KENSKPK KEE +KAEVRKVFGGLF QETIYVDDD
Sbjct: 541 SKENSKPKAKEE-AKAEVRKVFGGLFKQETIYVDDD 569

BLAST of MC02g0747 vs. NCBI nr
Match: XP_022946936.1 (protein plastid transcriptionally active 16, chloroplastic [Cucurbita moschata])

HSP 1 Score: 826 bits (2133), Expect = 1.34e-296
Identity = 482/581 (82.96%), Postives = 516/581 (88.81%), Query Frame = 0

Query: 1   MAAIITSNSFILTTAPNTRRSFKSH-RRQFAVYARRSGTFGSLRLGKSSNGSPSSDDAPP 60
           MAAII SNSFILTTAPN RRSF ++ RRQF+VYA+ SGTF S RLGKSSNGSPSSD AP 
Sbjct: 1   MAAIINSNSFILTTAPNARRSFDNNSRRQFSVYAKSSGTFASFRLGKSSNGSPSSDGAPA 60

Query: 61  EESGNSNPFRFNFGKVPDMKSLIPVVSNPSSSGLSFGNARRKDPNTVFVAGATGQAGIRL 120
           E+SGNSNPFRFNFGK+PD+KSLIPVVS+ +SSGLSFGNARRKDPNTVFVAGA+GQAGIRL
Sbjct: 61  EDSGNSNPFRFNFGKIPDVKSLIPVVSD-TSSGLSFGNARRKDPNTVFVAGASGQAGIRL 120

Query: 121 AQTLLREGFSVRAGVPELDAAQELARLAAKYMVISNEESKRLNAVESTFQDAESIAKAIG 180
           AQTLLREGFSVRAGVPELDAAQELARLAAKY VISNEESKRLNAVES+FQDAESIAKAIG
Sbjct: 121 AQTLLREGFSVRAGVPELDAAQELARLAAKYKVISNEESKRLNAVESSFQDAESIAKAIG 180

Query: 181 NAGKVVVTIGAAENGPTSEVTTSDALQVIQAAQLAGVSHVAIVYDGSALNSSTYNVLDGL 240
           NA KVVVTIGA ENGPTSEVTTSDALQVIQAAQLAGV HVAIVYDG+A NSST NVLDGL
Sbjct: 181 NASKVVVTIGAGENGPTSEVTTSDALQVIQAAQLAGVGHVAIVYDGNASNSSTNNVLDGL 240

Query: 241 SSFFNNLFSRSQQLSVSELLQKIVETDVGYTFIKTNLVEDFAPERTYNVVVGAEGSASSN 300
           SSFFNNLFSRSQ+LSV ELLQKIVETDV YTFIK NLVEDF PER YNVVV AEGSASSN
Sbjct: 241 SSFFNNLFSRSQELSVVELLQKIVETDVSYTFIKANLVEDFEPERAYNVVVRAEGSASSN 300

Query: 301 DYKVAKSQIASLVAGVFSNTAVAENKVVEVYSSPSAPSSSVEQLFSAIPKDGRREAYAEA 360
           DYKVAKS+IASLVAGVFSNTAVAENKVVEVYS+PSA SSSVEQLFSAIP DGRR+AYAEA
Sbjct: 301 DYKVAKSRIASLVAGVFSNTAVAENKVVEVYSTPSALSSSVEQLFSAIPTDGRRQAYAEA 360

Query: 361 QVKAKAEEEAIRAAEKARQEAEAAKKKEEQVAKRLSEKAAKASSSPPAAAAAEES----- 420
           Q KAKAEEEAIR AEKAR+EAEAAKK++ + AK  +EK A+A++   AAA AEES     
Sbjct: 361 QAKAKAEEEAIRTAEKAREEAEAAKKQQLEAAK--AEKTAQAAA---AAAVAEESQKQES 420

Query: 421 DQIAFFNTFLNKAKDFSSEQSQKLKKLSEKEPQAPSKAKAEEEAE----SAGSFVNNFFS 480
           D++AFFNT LNKAKDFSSEQSQKLKKLSEKEP+AP+K KAEE  E    +AGS VNNF S
Sbjct: 421 DEVAFFNTLLNKAKDFSSEQSQKLKKLSEKEPKAPTKPKAEEAPEKVDSAAGSLVNNFLS 480

Query: 481 KAKDLGSAQPWEKLAFQLQKPPSEESNVQVATVRGQAKARALPSKKAAVRQPQTNSNSKP 540
           KAK  GS QPW+KLAFQL K P EESNVQ+ATVRGQAKAR+LPSKKAAVRQ  TNS  KP
Sbjct: 481 KAKGFGSQQPWDKLAFQLPKQP-EESNVQIATVRGQAKARSLPSKKAAVRQAMTNS--KP 540

Query: 541 PFAVKAKENSKPKGKEEGSKAEVRKVFGGLFSQETIYVDDD 571
            FA K+KENSKPK KEE +KAEVRKVFGGLF QETIYVDDD
Sbjct: 541 SFAAKSKENSKPKAKEE-AKAEVRKVFGGLFKQETIYVDDD 571

BLAST of MC02g0747 vs. ExPASy TrEMBL
Match: A0A6J1D3Q1 (protein plastid transcriptionally active 16, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111017020 PE=4 SV=1)

HSP 1 Score: 1023 bits (2645), Expect = 0.0
Identity = 566/571 (99.12%), Postives = 568/571 (99.47%), Query Frame = 0

Query: 1   MAAIITSNSFILTTAPNTRRSFKSHRRQFAVYARRSGTFGSLRLGKSSNGSPSSDDAPPE 60
           MAAIITSNSFILTTAPNTRRSFKSHRRQFAVYARRSGTFGSLRLGKSSNGSPSSDDAPPE
Sbjct: 1   MAAIITSNSFILTTAPNTRRSFKSHRRQFAVYARRSGTFGSLRLGKSSNGSPSSDDAPPE 60

Query: 61  ESGNSNPFRFNFGKVPDMKSLIPVVSNPSSSGLSFGNARRKDPNTVFVAGATGQAGIRLA 120
           ESGNSNPFRFNFGKVPDMKSLIPVVSNPSSSGLSFGNARRKDPNTVFVAGATGQAGIRLA
Sbjct: 61  ESGNSNPFRFNFGKVPDMKSLIPVVSNPSSSGLSFGNARRKDPNTVFVAGATGQAGIRLA 120

Query: 121 QTLLREGFSVRAGVPELDAAQELARLAAKYMVISNEESKRLNAVESTFQDAESIAKAIGN 180
           QTLLREGFSVRAGVPELDAAQELARLAAKYMVISNEESKRLNAVESTFQDAESIAKAIGN
Sbjct: 121 QTLLREGFSVRAGVPELDAAQELARLAAKYMVISNEESKRLNAVESTFQDAESIAKAIGN 180

Query: 181 AGKVVVTIGAAENGPTSEVTTSDALQVIQAAQLAGVSHVAIVYDGSALNSSTYNVLDGLS 240
           AGKVVVTIGAAENGPTSEVTTSDALQVIQAAQLAGVSHVAIVYDGSALNSSTYNVLDGLS
Sbjct: 181 AGKVVVTIGAAENGPTSEVTTSDALQVIQAAQLAGVSHVAIVYDGSALNSSTYNVLDGLS 240

Query: 241 SFFNNLFSRSQQLSVSELLQKIVETDVGYTFIKTNLVEDFAPERTYNVVVGAEGSASSND 300
           SFFNNLFSRSQQLSVSELLQKIVETDVGYTFIKTNLVEDFAPERTYNVVVGAEGSASSND
Sbjct: 241 SFFNNLFSRSQQLSVSELLQKIVETDVGYTFIKTNLVEDFAPERTYNVVVGAEGSASSND 300

Query: 301 YKVAKSQIASLVAGVFSNTAVAENKVVEVYSSPSAPSSSVEQLFSAIPKDGRREAYAEAQ 360
           YKVAKSQIASLVAGVFSNTAVAENKVVEVYSSPSAPSSSVEQLFSAIPKDGRREAYAEAQ
Sbjct: 301 YKVAKSQIASLVAGVFSNTAVAENKVVEVYSSPSAPSSSVEQLFSAIPKDGRREAYAEAQ 360

Query: 361 VKAKAEEEAIRAAEKARQEAEAAKKKEEQVAKRLSEKAAKASSSPPAAAAAEESDQIAFF 420
            KAKAEEEAIRAAEKARQEAEAAKKKEEQVAKRLSEKAAKASS PPAAAA EESDQIAFF
Sbjct: 361 AKAKAEEEAIRAAEKARQEAEAAKKKEEQVAKRLSEKAAKASSPPPAAAA-EESDQIAFF 420

Query: 421 NTFLNKAKDFSSEQSQKLKKLSEKEPQAPSKAKAEEEAESAGSFVNNFFSKAKDLGSAQP 480
           NTFLNKAKDFSSEQSQKLKKL+EKEPQAPSKAKAEEEAESAGSFVNNFFSKAKDLGSAQP
Sbjct: 421 NTFLNKAKDFSSEQSQKLKKLTEKEPQAPSKAKAEEEAESAGSFVNNFFSKAKDLGSAQP 480

Query: 481 WEKLAFQLQKPPSEESNVQVATVRGQAKARALPSKKAAVRQPQTNSNSKPPFAVKAKENS 540
           WEKLAFQLQKPPSEESNVQVATVRGQAKARALPSKKAAVRQPQTNSNSKPPFAVKAKENS
Sbjct: 481 WEKLAFQLQKPPSEESNVQVATVRGQAKARALPSKKAAVRQPQTNSNSKPPFAVKAKENS 540

Query: 541 KPKGKEEGSKAEVRKVFGGLFSQETIYVDDD 571
           KPKGKEEGSKAEVRKVFGGLFSQET+YVDDD
Sbjct: 541 KPKGKEEGSKAEVRKVFGGLFSQETLYVDDD 570

BLAST of MC02g0747 vs. ExPASy TrEMBL
Match: A0A6J1G4Z0 (protein plastid transcriptionally active 16, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111450967 PE=4 SV=1)

HSP 1 Score: 826 bits (2133), Expect = 6.48e-297
Identity = 482/581 (82.96%), Postives = 516/581 (88.81%), Query Frame = 0

Query: 1   MAAIITSNSFILTTAPNTRRSFKSH-RRQFAVYARRSGTFGSLRLGKSSNGSPSSDDAPP 60
           MAAII SNSFILTTAPN RRSF ++ RRQF+VYA+ SGTF S RLGKSSNGSPSSD AP 
Sbjct: 1   MAAIINSNSFILTTAPNARRSFDNNSRRQFSVYAKSSGTFASFRLGKSSNGSPSSDGAPA 60

Query: 61  EESGNSNPFRFNFGKVPDMKSLIPVVSNPSSSGLSFGNARRKDPNTVFVAGATGQAGIRL 120
           E+SGNSNPFRFNFGK+PD+KSLIPVVS+ +SSGLSFGNARRKDPNTVFVAGA+GQAGIRL
Sbjct: 61  EDSGNSNPFRFNFGKIPDVKSLIPVVSD-TSSGLSFGNARRKDPNTVFVAGASGQAGIRL 120

Query: 121 AQTLLREGFSVRAGVPELDAAQELARLAAKYMVISNEESKRLNAVESTFQDAESIAKAIG 180
           AQTLLREGFSVRAGVPELDAAQELARLAAKY VISNEESKRLNAVES+FQDAESIAKAIG
Sbjct: 121 AQTLLREGFSVRAGVPELDAAQELARLAAKYKVISNEESKRLNAVESSFQDAESIAKAIG 180

Query: 181 NAGKVVVTIGAAENGPTSEVTTSDALQVIQAAQLAGVSHVAIVYDGSALNSSTYNVLDGL 240
           NA KVVVTIGA ENGPTSEVTTSDALQVIQAAQLAGV HVAIVYDG+A NSST NVLDGL
Sbjct: 181 NASKVVVTIGAGENGPTSEVTTSDALQVIQAAQLAGVGHVAIVYDGNASNSSTNNVLDGL 240

Query: 241 SSFFNNLFSRSQQLSVSELLQKIVETDVGYTFIKTNLVEDFAPERTYNVVVGAEGSASSN 300
           SSFFNNLFSRSQ+LSV ELLQKIVETDV YTFIK NLVEDF PER YNVVV AEGSASSN
Sbjct: 241 SSFFNNLFSRSQELSVVELLQKIVETDVSYTFIKANLVEDFEPERAYNVVVRAEGSASSN 300

Query: 301 DYKVAKSQIASLVAGVFSNTAVAENKVVEVYSSPSAPSSSVEQLFSAIPKDGRREAYAEA 360
           DYKVAKS+IASLVAGVFSNTAVAENKVVEVYS+PSA SSSVEQLFSAIP DGRR+AYAEA
Sbjct: 301 DYKVAKSRIASLVAGVFSNTAVAENKVVEVYSTPSALSSSVEQLFSAIPTDGRRQAYAEA 360

Query: 361 QVKAKAEEEAIRAAEKARQEAEAAKKKEEQVAKRLSEKAAKASSSPPAAAAAEES----- 420
           Q KAKAEEEAIR AEKAR+EAEAAKK++ + AK  +EK A+A++   AAA AEES     
Sbjct: 361 QAKAKAEEEAIRTAEKAREEAEAAKKQQLEAAK--AEKTAQAAA---AAAVAEESQKQES 420

Query: 421 DQIAFFNTFLNKAKDFSSEQSQKLKKLSEKEPQAPSKAKAEEEAE----SAGSFVNNFFS 480
           D++AFFNT LNKAKDFSSEQSQKLKKLSEKEP+AP+K KAEE  E    +AGS VNNF S
Sbjct: 421 DEVAFFNTLLNKAKDFSSEQSQKLKKLSEKEPKAPTKPKAEEAPEKVDSAAGSLVNNFLS 480

Query: 481 KAKDLGSAQPWEKLAFQLQKPPSEESNVQVATVRGQAKARALPSKKAAVRQPQTNSNSKP 540
           KAK  GS QPW+KLAFQL K P EESNVQ+ATVRGQAKAR+LPSKKAAVRQ  TNS  KP
Sbjct: 481 KAKGFGSQQPWDKLAFQLPKQP-EESNVQIATVRGQAKARSLPSKKAAVRQAMTNS--KP 540

Query: 541 PFAVKAKENSKPKGKEEGSKAEVRKVFGGLFSQETIYVDDD 571
            FA K+KENSKPK KEE +KAEVRKVFGGLF QETIYVDDD
Sbjct: 541 SFAAKSKENSKPKAKEE-AKAEVRKVFGGLFKQETIYVDDD 571

BLAST of MC02g0747 vs. ExPASy TrEMBL
Match: A0A0A0KC77 (NAD(P)-bd_dom domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G188160 PE=4 SV=1)

HSP 1 Score: 822 bits (2124), Expect = 2.04e-295
Identity = 476/590 (80.68%), Postives = 512/590 (86.78%), Query Frame = 0

Query: 1   MAAIITSNSFILTTAPNTRRSFKSHRRQFAVYARRSGTFGSLRLGKSSNGSPSSDDAPPE 60
           MAAII SNSFILTT PNTRRSFKSHRRQ AV+A+ SG+F S RLGKSSN SPSSDDAP E
Sbjct: 1   MAAIINSNSFILTTTPNTRRSFKSHRRQVAVFAKNSGSFSSFRLGKSSNESPSSDDAPVE 60

Query: 61  ESGNSNPFRFNFGKVPDMKSLIPVVSNPSSSGLSFGNARRKDPNTVFVAGATGQAGIRLA 120
           +SGNSNPFRF+FGKVPD+KSLIPVVS PSS GLSFG++RRKD NTVFVAGATGQAGIRLA
Sbjct: 61  DSGNSNPFRFSFGKVPDVKSLIPVVSEPSS-GLSFGSSRRKDSNTVFVAGATGQAGIRLA 120

Query: 121 QTLLREGFSVRAGVPELDAAQELARLAAKYMVISNEESKRLNAVESTFQDAESIAKAIGN 180
           QTLLREGFSVRAGVPEL AAQELARLAAKY VISNEESKRLNAVES+FQDAE+IAKAIGN
Sbjct: 121 QTLLREGFSVRAGVPELGAAQELARLAAKYKVISNEESKRLNAVESSFQDAEAIAKAIGN 180

Query: 181 AGKVVVTIGAAENGPTSEVTTSDALQVIQAAQLAGVSHVAIVYDGSALNSSTYNVLDGLS 240
           A KVVVTIG  ENGPTSEVTTSDALQVIQAAQLAGVSHVA+VYDG+A +SSTYNVLDGLS
Sbjct: 181 ASKVVVTIGVGENGPTSEVTTSDALQVIQAAQLAGVSHVAVVYDGNASSSSTYNVLDGLS 240

Query: 241 SFFNNLFSRSQQLSVSELLQKIVETDVGYTFIKTNLVEDFAPERTYNVVVGAEGSASSND 300
           SFFNNLFSRSQ LSV ELLQKIVETD+GYTFIKTNLVEDFAPER YNVVV AEGSASSND
Sbjct: 241 SFFNNLFSRSQPLSVVELLQKIVETDIGYTFIKTNLVEDFAPERAYNVVVQAEGSASSND 300

Query: 301 YKVAKSQIASLVAGVFSNTAVAENKVVEVYSSPSAPSSSVEQLFSAIPKDGRREAYAEAQ 360
           YKVA+SQIASLVAGVFSNTAVAENKVVEVYSSPSAPSSSV+QLFS IP DGRR+AYAEA+
Sbjct: 301 YKVAQSQIASLVAGVFSNTAVAENKVVEVYSSPSAPSSSVDQLFSVIPTDGRRQAYAEAK 360

Query: 361 VKAKAEEEAIRAAEKARQEAEAAKKKEEQVAK--RLSEKAA----------KASSSPPAA 420
            KAKAEEEAIR AE AR+EAEA KK+E + AK  RLSEKAA           +SSSPPA 
Sbjct: 361 AKAKAEEEAIRIAENAREEAEATKKQEVEAAKSKRLSEKAATQPSSSSSSSSSSSSPPA- 420

Query: 421 AAAEESDQIAFFNTFLNKAKDFSSEQSQKLKKLSEKEPQAPSKAKAEEEAESAGSFVNNF 480
              EESDQIAFFN+FLNKAKDFSSEQSQKLKKLSEKEPQ       EE  ++AG+ VNNF
Sbjct: 421 ---EESDQIAFFNSFLNKAKDFSSEQSQKLKKLSEKEPQETE----EESPDTAGNLVNNF 480

Query: 481 FSKAKDLGSAQPWEKLAFQLQKPPSEESNVQVATVRGQAKARALPSKKAAVRQPQTNSNS 540
           F+KAK  GSAQPWEKL+FQLQKP SEESN Q+ATVRGQAKARALP KKA++RQ Q  +NS
Sbjct: 481 FNKAKGFGSAQPWEKLSFQLQKP-SEESNAQIATVRGQAKARALPPKKASIRQTQ-KTNS 540

Query: 541 KPPFAVKAKENSKPKGK-------EEGSKAEVRKVFGGLFSQETIYVDDD 571
           KP F +K KENSKPK         +E +KAEVRKVFGGLF QETIYVDD+
Sbjct: 541 KPSFGLKQKENSKPKAAATAAAAAKEETKAEVRKVFGGLFKQETIYVDDE 579

BLAST of MC02g0747 vs. ExPASy TrEMBL
Match: A0A6J1KZG1 (protein plastid transcriptionally active 16, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111499653 PE=4 SV=1)

HSP 1 Score: 820 bits (2117), Expect = 1.58e-294
Identity = 476/581 (81.93%), Postives = 509/581 (87.61%), Query Frame = 0

Query: 1   MAAIITSNSFILTTAPNTRRSFKSH-RRQFAVYARRSGTFGSLRLGKSSNGSPSSDDAPP 60
           MAAII SNSFILTTAPN RRSF ++ RRQF+VYA+ SGTF S RLGKSSNGSPSSD AP 
Sbjct: 1   MAAIINSNSFILTTAPNARRSFDNNSRRQFSVYAKSSGTFASFRLGKSSNGSPSSDGAPA 60

Query: 61  EESGNSNPFRFNFGKVPDMKSLIPVVSNPSSSGLSFGNARRKDPNTVFVAGATGQAGIRL 120
           E+SGNSNP+RFNFGK+PD+KSLIPVVS  +SSGLSFGN+RRKDPNTVFVAGA+GQAGIRL
Sbjct: 61  EDSGNSNPYRFNFGKIPDVKSLIPVVSE-TSSGLSFGNSRRKDPNTVFVAGASGQAGIRL 120

Query: 121 AQTLLREGFSVRAGVPELDAAQELARLAAKYMVISNEESKRLNAVESTFQDAESIAKAIG 180
           AQTLLREGFSVRAGVPELDAAQELARLAAKY VISNEESKRLNAVES+FQDAESIAKAIG
Sbjct: 121 AQTLLREGFSVRAGVPELDAAQELARLAAKYKVISNEESKRLNAVESSFQDAESIAKAIG 180

Query: 181 NAGKVVVTIGAAENGPTSEVTTSDALQVIQAAQLAGVSHVAIVYDGSALNSSTYNVLDGL 240
           NA KVVVTIGA ENGPTSEVTTSDALQVIQAAQLAGV HVAIVYDG+A NSST NVLDG+
Sbjct: 181 NASKVVVTIGAGENGPTSEVTTSDALQVIQAAQLAGVGHVAIVYDGNASNSSTNNVLDGI 240

Query: 241 SSFFNNLFSRSQQLSVSELLQKIVETDVGYTFIKTNLVEDFAPERTYNVVVGAEGSASSN 300
           SSFFNNLFSRSQ LSV ELLQKIVETD+ YTFIK NL EDF PER YNVVVGAEGSASSN
Sbjct: 241 SSFFNNLFSRSQALSVVELLQKIVETDLSYTFIKANLAEDFEPERAYNVVVGAEGSASSN 300

Query: 301 DYKVAKSQIASLVAGVFSNTAVAENKVVEVYSSPSAPSSSVEQLFSAIPKDGRREAYAEA 360
           DYKVAKS+IASLVAGVFSNTAVAENKVVEVYS+PSAPSSSVEQLFSAIP DGRR+AYAEA
Sbjct: 301 DYKVAKSRIASLVAGVFSNTAVAENKVVEVYSTPSAPSSSVEQLFSAIPTDGRRQAYAEA 360

Query: 361 QVKAKAEEEAIRAAEKARQEAEAAKKKEEQVAKRLSEKAAKASSSPPAAAAAEES----- 420
           Q KAKAEEEAIR AE+AR+EAEAAKK++ +        AAKA  +  AAA AEES     
Sbjct: 361 QAKAKAEEEAIRTAEQAREEAEAAKKQQLE--------AAKAEKTAQAAAVAEESQKQES 420

Query: 421 DQIAFFNTFLNKAKDFSSEQSQKLKKLSEKEPQAPSKAKAEEEAE----SAGSFVNNFFS 480
           D++AFFNT LNKAKDFSSEQSQKLKKLSEKEP+AP+K KAEE  E    +AGS VNNF S
Sbjct: 421 DEVAFFNTLLNKAKDFSSEQSQKLKKLSEKEPKAPTKPKAEEAPEKADSAAGSLVNNFLS 480

Query: 481 KAKDLGSAQPWEKLAFQLQKPPSEESNVQVATVRGQAKARALPSKKAAVRQPQTNSNSKP 540
           KAK  G  QPWEKLAFQL K  SEESNVQ+ATVRGQAKAR+LPSKKAAVRQ  TNS  KP
Sbjct: 481 KAKGFGLQQPWEKLAFQLPKQ-SEESNVQIATVRGQAKARSLPSKKAAVRQAMTNS--KP 540

Query: 541 PFAVKAKENSKPKGKEEGSKAEVRKVFGGLFSQETIYVDDD 571
            FA K+KENSKPK KEE +KAEVRKVFGGLF QETIYVDDD
Sbjct: 541 SFAAKSKENSKPKPKEE-AKAEVRKVFGGLFKQETIYVDDD 568

BLAST of MC02g0747 vs. ExPASy TrEMBL
Match: A0A5D3CYY3 (Protein plastid transcriptionally active 16 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold571G00290 PE=4 SV=1)

HSP 1 Score: 798 bits (2061), Expect = 8.66e-286
Identity = 470/591 (79.53%), Postives = 504/591 (85.28%), Query Frame = 0

Query: 1   MAAIITSNSFILTTAPNTRRSFKSH-RRQFAVYARRSGTFGSLRLGKSSNGSPSSDDAPP 60
           MAAII SNSFILTT PN RRSFK+H RRQFAV+A+ SG+F S RLGKSSN SPSS D PP
Sbjct: 1   MAAIINSNSFILTTTPNARRSFKTHDRRQFAVHAKNSGSFSSFRLGKSSNESPSSSDDPP 60

Query: 61  -EESGNSNPFRFNFGKVPDMKSLIPVVSNPSSSGLSFGNARRKDPNTVFVAGATGQAGIR 120
            E+SGNSNPFRF FGKVPD+KSLIPVVS PSS GLSFGN+RRKD NTVFVAGATGQAGIR
Sbjct: 61  LEDSGNSNPFRFPFGKVPDVKSLIPVVSEPSS-GLSFGNSRRKDSNTVFVAGATGQAGIR 120

Query: 121 LAQTLLREGFSVRAGVPELDAAQELARLAAKYMVISNEESKRLNAVESTFQDAESIAKAI 180
           LAQTLLREGFSVRAGVPEL AAQELARLAAKY VISNEESKRLNAVES+FQDAE+IAKAI
Sbjct: 121 LAQTLLREGFSVRAGVPELGAAQELARLAAKYKVISNEESKRLNAVESSFQDAEAIAKAI 180

Query: 181 GNAGKVVVTIGAAENGPTSEVTTSDALQVIQAAQLAGVSHVAIVYDGSALNSSTYNVLDG 240
           GNA KVVVTIGA ENGPTSEVTTSDALQVIQAA+LAGVSHVA+VYDG+  +SSTYNVLDG
Sbjct: 181 GNASKVVVTIGAGENGPTSEVTTSDALQVIQAAELAGVSHVAVVYDGNTSSSSTYNVLDG 240

Query: 241 LSSFFNNLFSRSQQLSVSELLQKIVETDVGYTFIKTNLVEDFAPERTYNVVVGAEGSASS 300
           LSSFFNNLFSRSQ LSV ELLQKI ETD+GYTFIKTNLVEDFAPER YNVVV AEGSASS
Sbjct: 241 LSSFFNNLFSRSQPLSVVELLQKIAETDIGYTFIKTNLVEDFAPERAYNVVVQAEGSASS 300

Query: 301 NDYKVAKSQIASLVAGVFSNTAVAENKVVEVYSSPSAPSSSVEQLFSAIPKDGRREAYAE 360
           NDYKVAKSQIASLVAGVFSNTAVAENKVVEVYSSPSA SSSV+QLFS IP DGRR+AYAE
Sbjct: 301 NDYKVAKSQIASLVAGVFSNTAVAENKVVEVYSSPSASSSSVDQLFSVIPTDGRRQAYAE 360

Query: 361 AQVKAKAEEEAIRAAEKARQEAEAAKKKEEQVAK--RLSEKAAKA-----------SSSP 420
           A  KAKAEEEA+R AEKAR+EAEA KK+E + AK  R SEKAA             SSSP
Sbjct: 361 A--KAKAEEEAMRIAEKAREEAEATKKQEVEAAKSKRPSEKAATQPSSSSSSSSSFSSSP 420

Query: 421 PAAAAAEESDQIAFFNTFLNKAKDFSSEQSQKLKKLSEKEPQAPSKAKAEEEAESAGSFV 480
           P    A+ESDQIAFFN+FLNKAKDFSSEQS KLKKLSEKEPQ       EE  ++A + V
Sbjct: 421 PPPPLAQESDQIAFFNSFLNKAKDFSSEQSLKLKKLSEKEPQETE----EESPDTAANLV 480

Query: 481 NNFFSKAKDLGSAQPWEKLAFQLQKPPSEESNVQVATVRGQAKARALPSKKAAVRQPQTN 540
           NNFF+KAK  GSAQPWEKL+FQLQKP SEESN Q+ATVRGQAKARALP KKA++RQ Q  
Sbjct: 481 NNFFNKAKGFGSAQPWEKLSFQLQKP-SEESNAQIATVRGQAKARALPPKKASIRQTQ-K 540

Query: 541 SNSKPPFAVKAKENSKPKGK-----EEGSKAEVRKVFGGLFSQETIYVDDD 571
           +NSKP F +K KENSKPK       +E +KAEVRKVFGGLF QETIYVDDD
Sbjct: 541 TNSKPSFGLKQKENSKPKAAAAAAAKEETKAEVRKVFGGLFKQETIYVDDD 582

BLAST of MC02g0747 vs. TAIR 10
Match: AT3G46780.1 (plastid transcriptionally active 16 )

HSP 1 Score: 500.0 bits (1286), Expect = 2.6e-141
Identity = 316/576 (54.86%), Postives = 403/576 (69.97%), Query Frame = 0

Query: 6   TSNSFILTTAPNTRRSFKSHRRQFAVYARRSGTFGSLRLGKSSNGSPSSDDAPPEESGNS 65
           +S SF LTTAP     F   + +  V+A+++    + +LGK+       DD+  ++ G  
Sbjct: 4   SSTSFPLTTAPPQGVRFNRRKPRLTVWAKQT----AFQLGKTK----GDDDSEGKQKG-K 63

Query: 66  NPFRFNFGKVPDMKSLIPVVSNPSSSGLSFGNARRKDPNTVFVAGATGQAGIRLAQTLLR 125
           NPF+F+FGK+PDMKSLIPVV+NP S+GL FGN R+KDP T+FVAGATGQAGIR+AQTLL+
Sbjct: 64  NPFQFDFGKLPDMKSLIPVVTNP-STGLVFGNNRKKDPGTIFVAGATGQAGIRIAQTLLQ 123

Query: 126 EGFSVRAGVPELDAAQELARLAAKYMVISNEESKRLNAVESTFQDAESIAKAIGNAGKVV 185
            GFSVRAGVP+L AAQ+LAR+AA Y ++SN+E KRLNAV+S FQDAESIAKAIGNA KVV
Sbjct: 124 RGFSVRAGVPDLGAAQDLARVAATYKILSNDEVKRLNAVQSPFQDAESIAKAIGNATKVV 183

Query: 186 VTIGAAENGPTSEVTTSDALQVIQAAQLAGVSHVAIVYDGSALNSSTYNVLDGLSSFFNN 245
           VT+GA ENGP ++V+TSDAL V+QAA+LAGVSHVAIVYDG+ ++ STYNVLDG++SFF N
Sbjct: 184 VTVGATENGPDAQVSTSDALLVVQAAELAGVSHVAIVYDGT-ISGSTYNVLDGITSFFGN 243

Query: 246 LFSRSQQLSVSELLQKIVETDVGYTFIKTNLVEDFAPERTYNVVVGAE------GSASSN 305
           LF++SQ L++S+L++K+ +TDV YT IKT+L EDF+PE+ YNVVV AE      GS+SS 
Sbjct: 244 LFAKSQPLTISDLIEKVAQTDVAYTLIKTSLTEDFSPEKAYNVVVSAEGSNSGSGSSSSE 303

Query: 306 DYKVAKSQIASLVAGVFSNTAVAENKVVEVYSSPSAPSSSVEQLFSAIPKDGRREAYAEA 365
            YKV K +IASLVA +F+NTAVAENKVVEV + PSAPS  V++LFS IP+DGRR+ YA+A
Sbjct: 304 AYKVPKLKIASLVADIFANTAVAENKVVEVSTDPSAPSRPVDELFSVIPEDGRRKVYADA 363

Query: 366 QVKAKAEEEAIRAAEKARQEAEAAKKKEEQVAKRLSEKAAKASSSPPAAAAAEESDQIAF 425
             + +AEEEA  AA+KAR+ AEAAK+ E+Q                              
Sbjct: 364 IARERAEEEAKVAADKAREAAEAAKEFEKQ------------------------------ 423

Query: 426 FNTFLNKAKDFSSEQSQKLKKLSEKEPQAPSKAK-AEEEAESAGSFVNNFFSKAKDLGSA 485
                             ++KLSEKE +A S A+ A+++A++ G  V+  F+KAKD+ S 
Sbjct: 424 ------------------MQKLSEKEAEAASLAEDAQQKADAVGVTVDGLFNKAKDISSG 483

Query: 486 QPWEKLAFQLQ---KPPSEESNVQVATVRGQAKARALPSKKAAVRQPQTNSNSKPPFAVK 545
             W KL  Q     +  SE   VQVATVRGQAKAR LP KKA V+Q  ++     PFA K
Sbjct: 484 LSWNKLGSQFATAIQNASETPKVQVATVRGQAKARNLPPKKAVVKQRPSS-----PFASK 510

Query: 546 AKENSKPKGKEEGSKAEVRKVFGGLFSQETIYVDDD 572
            KE  +PK  E+    EVRKVFGGLF QETIY+DDD
Sbjct: 544 PKE-ERPKKPEK----EVRKVFGGLFKQETIYIDDD 510

BLAST of MC02g0747 vs. TAIR 10
Match: AT3G18890.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 60.5 bits (145), Expect = 5.2e-09
Identity = 83/339 (24.48%), Postives = 158/339 (46.61%), Query Frame = 0

Query: 97  NARRKDPNTVFVAGATGQAGIRLAQTLLREGFSVRAGVPELDAAQELARLAAKYMVISNE 156
           N   K+ + VFVAGATG+ G R  + LL+ GF VRAGV     A  L + + K M + N 
Sbjct: 75  NLNSKEEDLVFVAGATGKVGSRTVRELLKLGFRVRAGVRSAQRAGSLVQ-SVKEMKLQNT 134

Query: 157 ES-----KRLNAVESTFQDAESIAKAIGNAGKVVVTIGAAENGPTSEVTTSDAL------ 216
           +      ++L  VE   +  +SI  A+GNA  ++  IGA+E    S++T    +      
Sbjct: 135 DEGTQPVEKLEIVECDLEKKDSIQPALGNASVIICCIGASEK-EISDITGPYRIDYLATK 194

Query: 217 QVIQAAQLAGVSHVAIVYDGSALNSSTYNVLDGLSSFFNNLFSRSQQLSVSELLQKIVET 276
            ++ AA  A V++  +V   ++L ++ +     + + F  +    ++       + ++E+
Sbjct: 195 NLVDAATSAKVNNFILV---TSLGTNKFGFPAAILNLFWGVLCWKRKAE-----EALIES 254

Query: 277 DVGYTFIKTNLVE--DFAPERTYNVVVGAEGSASSNDYKVAKSQIASLVAGVFSNTAVAE 336
            + Y  ++   +E    A + T+N+ +  + +      +V+  Q+A L+A +  N  ++ 
Sbjct: 255 GLNYAIVRPGGMERPTDAYKETHNLTLALDDTLFGG--QVSNLQVAELLACMAKNPQLSF 314

Query: 337 NKVVEVYSSPSAPSSSVEQLFSAIPKDGRREAYAEAQVKAK----AEEEAIRAAEKARQE 396
           +K+VEV +  +AP + +E+L   IP         +A V  K       + +     A +E
Sbjct: 315 SKIVEVVAETTAPLTPIEKLLEKIPSKRPYVPPPKASVATKEVKPVPTKPVTQEPTAPKE 374

Query: 397 AEAAKKKEEQVAKRLSEKAAKASSSPPAAAAAEESDQIA 419
            EA  K++    + LS  A+     PP +     +  ++
Sbjct: 375 DEAPPKEKNVKPRPLSPYASYEDLKPPTSPIPNSTTSVS 401

BLAST of MC02g0747 vs. TAIR 10
Match: AT2G34460.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 47.4 bits (111), Expect = 4.6e-05
Identity = 64/252 (25.40%), Postives = 112/252 (44.44%), Query Frame = 0

Query: 106 VFVAGATGQAGIRLAQTLLREGFSVRAGVPELDAAQELARLAAKYMVISNEESKRLNAVE 165
           VFVAGATGQ G R+ + LL  GF+V+AGV +++ A+            S ++   L  V 
Sbjct: 49  VFVAGATGQTGKRIVEQLLSRGFAVKAGVRDVEKAK-----------TSFKDDPSLQIVR 108

Query: 166 STFQDA-ESIAKAIGNAGKVVVTIGAAENG----PTSEVTTSDALQVIQAAQLAGVSHVA 225
           +   +  + +A+ IG+  + V+       G       +V     + ++ A +  GV    
Sbjct: 109 ADVTEGPDKLAEVIGDDSQAVICATGFRPGFDIFTPWKVDNFGTVNLVDACRKQGVEKFV 168

Query: 226 IVYDGSALNSSTYNVLDGLSSFFNNLFSRS--QQLSVSELLQKIVETDVGYTFIKTNLVE 285
           +V     +N +    +   +  F NLF  +   +L   + ++K   + + YT ++   ++
Sbjct: 169 LV-SSILVNGAAMGQILNPAYLFLNLFGLTLVAKLQAEKYIKK---SGINYTIVRPGGLK 228

Query: 286 DFAPERTYNVVVGAEGSASSNDYKVAKSQIASLVAGVFSNTAVAEN---KVVEVYSSPSA 345
           +  P  T NVV+  E      D     S    LVA V     + E    KVVE+ +   A
Sbjct: 229 NDPP--TGNVVMEPE------DTLYEGSISRDLVAEVAVEALLQEESSFKVVEIVARAEA 277

Query: 346 PSSSVEQLFSAI 348
           P  S + LF+++
Sbjct: 289 PKRSYKDLFASV 277