MC02g0479 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC02g0479
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	O-methyltransferase
Location	MC02: 3976077 .. 3977403 (-)
RNA-Seq Expression	MC02g0479
Synteny	MC02g0479

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

GAGATGGAACCCAAAGAAGCAAATGAAGCAGAGGCAATGCTTAGAGGGCAAGCAGACATATGGAGATATATGCTCTGTTTTGCGGATTCAATGGCCCTCAAATCTGCCGTCCAACTTCGCTTAGCTGACATCATCAAATCTCACGGCTCTCCCCTCACTCTCTCTCAGATTGCCGCCTCCATTGCTTCTTCCTCTGCCTCTCCAAACATTTCCTTCCTCGCTCGCATCATGGCACTCCTCGTCCGCCGCAACATCTTCGCCGCACACCACCCATCCGACGGCGGCGAAACCCTCTACGGCCTCACCCACTCCTCCCGCTGGCTCCTCCGCGACTCCGACCTCACCCTCGCCCCCATGATACTCGCCGAGCTTCACCCCTGGCTCCTCGATCCATGGCACCGTTTCGCCCAAGGCGTCAGAGAAGGCGGCAACCTCTTCCACAAGGCCAACGGCCTCCATTTCTGGGACTTCGCTGCCAGAAACCCCCAATTCAACCGATTGTTCAACGACGCCATGGCCTCCACTTCCAAGGTCGTCATTAACGCAATCCTCTCTGTTTACAAAGACGGGTTCAACTCGGTGGGGTCGCTGGCGGACGTCGGCGGCGGAATCGGGGGCGCCGTGTCGGAGATCGTGAAAGCGTACCCCCACATGAAGGGCATCAACTACGACTTGCCGCACGTGGTGAGCACGGCGCCGGCGTACCCAGGGGTGACCCACGTGGGAGGCGACATGTTTGAGAGCGTTCCCAAGGCCGACGCCATTTTTATGAAGGTGATCCATTAAACTAATCAATAAATTGCATCCATTAATTGGTATAATTATGAGTTGAATTGGTTAATGGTACAGTGGATACTGCACGATTGGGACGACAAGGAGTGCGTGAGAATTCTGGAGAATTGCAAGAAGGCGATACCGGAGAAAACAGGGAAAGTGATTATAGTGGAAGTGGTGCTGAATCCGGAAGGAGAGGGCCCATTTGACGACACTGGCTTGTATTTTGATCTGCTAATGTTGGTGCATTCCAACGGAAAAGAGAGGACAGAGAAGGAGTGGAAGAAAATATTGGAGGAAGCAGGGTTCCTTCGCTACAAACTCCTTGCTCTTCCTGCAATAACTTCAATTATTGAAGCTTATCCTTTCTAAATTTAGAGGATCTCTCAATGTATTACTTCTGGTTTAGTCGTAAGATCCTCCATTTTTTTTGTTTTAATATATTTACCTTTGAGAAGGGAAAAAAAAACTATTCAAAGATCTCGTTGTATAAGGTTTAAAAAAATTTAACTTTAGTTTGAATGATAACTTATTTTTAAAAATTTCATCTGGC

mRNA sequence

GAGATGGAACCCAAAGAAGCAAATGAAGCAGAGGCAATGCTTAGAGGGCAAGCAGACATATGGAGATATATGCTCTGTTTTGCGGATTCAATGGCCCTCAAATCTGCCGTCCAACTTCGCTTAGCTGACATCATCAAATCTCACGGCTCTCCCCTCACTCTCTCTCAGATTGCCGCCTCCATTGCTTCTTCCTCTGCCTCTCCAAACATTTCCTTCCTCGCTCGCATCATGGCACTCCTCGTCCGCCGCAACATCTTCGCCGCACACCACCCATCCGACGGCGGCGAAACCCTCTACGGCCTCACCCACTCCTCCCGCTGGCTCCTCCGCGACTCCGACCTCACCCTCGCCCCCATGATACTCGCCGAGCTTCACCCCTGGCTCCTCGATCCATGGCACCGTTTCGCCCAAGGCGTCAGAGAAGGCGGCAACCTCTTCCACAAGGCCAACGGCCTCCATTTCTGGGACTTCGCTGCCAGAAACCCCCAATTCAACCGATTGTTCAACGACGCCATGGCCTCCACTTCCAAGGTCGTCATTAACGCAATCCTCTCTGTTTACAAAGACGGGTTCAACTCGGTGGGGTCGCTGGCGGACGTCGGCGGCGGAATCGGGGGCGCCGTGTCGGAGATCGTGAAAGCGTACCCCCACATGAAGGGCATCAACTACGACTTGCCGCACGTGGTGAGCACGGCGCCGGCGTACCCAGGGGTGACCCACGTGGGAGGCGACATGTTTGAGAGCGTTCCCAAGGCCGACGCCATTTTTATGAAGTGGATACTGCACGATTGGGACGACAAGGAGTGCGTGAGAATTCTGGAGAATTGCAAGAAGGCGATACCGGAGAAAACAGGGAAAGTGATTATAGTGGAAGTGGTGCTGAATCCGGAAGGAGAGGGCCCATTTGACGACACTGGCTTGTATTTTGATCTGCTAATGTTGGTGCATTCCAACGGAAAAGAGAGGACAGAGAAGGAGTGGAAGAAAATATTGGAGGAAGCAGGGTTCCTTCGCTACAAACTCCTTGCTCTTCCTGCAATAACTTCAATTATTGAAGCTTATCCTTTCTAAATTTAGAGGATCTCTCAATGTATTACTTCTGGTTTAGTCGTAAGATCCTCCATTTTTTTTGTTTTAATATATTTACCTTTGAGAAGGGAAAAAAAAACTATTCAAAGATCTCGTTGTATAAGGTTTAAAAAAATTTAACTTTAGTTTGAATGATAACTTATTTTTAAAAATTTCATCTGGC

Coding sequence (CDS)

GAGATGGAACCCAAAGAAGCAAATGAAGCAGAGGCAATGCTTAGAGGGCAAGCAGACATATGGAGATATATGCTCTGTTTTGCGGATTCAATGGCCCTCAAATCTGCCGTCCAACTTCGCTTAGCTGACATCATCAAATCTCACGGCTCTCCCCTCACTCTCTCTCAGATTGCCGCCTCCATTGCTTCTTCCTCTGCCTCTCCAAACATTTCCTTCCTCGCTCGCATCATGGCACTCCTCGTCCGCCGCAACATCTTCGCCGCACACCACCCATCCGACGGCGGCGAAACCCTCTACGGCCTCACCCACTCCTCCCGCTGGCTCCTCCGCGACTCCGACCTCACCCTCGCCCCCATGATACTCGCCGAGCTTCACCCCTGGCTCCTCGATCCATGGCACCGTTTCGCCCAAGGCGTCAGAGAAGGCGGCAACCTCTTCCACAAGGCCAACGGCCTCCATTTCTGGGACTTCGCTGCCAGAAACCCCCAATTCAACCGATTGTTCAACGACGCCATGGCCTCCACTTCCAAGGTCGTCATTAACGCAATCCTCTCTGTTTACAAAGACGGGTTCAACTCGGTGGGGTCGCTGGCGGACGTCGGCGGCGGAATCGGGGGCGCCGTGTCGGAGATCGTGAAAGCGTACCCCCACATGAAGGGCATCAACTACGACTTGCCGCACGTGGTGAGCACGGCGCCGGCGTACCCAGGGGTGACCCACGTGGGAGGCGACATGTTTGAGAGCGTTCCCAAGGCCGACGCCATTTTTATGAAGTGGATACTGCACGATTGGGACGACAAGGAGTGCGTGAGAATTCTGGAGAATTGCAAGAAGGCGATACCGGAGAAAACAGGGAAAGTGATTATAGTGGAAGTGGTGCTGAATCCGGAAGGAGAGGGCCCATTTGACGACACTGGCTTGTATTTTGATCTGCTAATGTTGGTGCATTCCAACGGAAAAGAGAGGACAGAGAAGGAGTGGAAGAAAATATTGGAGGAAGCAGGGTTCCTTCGCTACAAACTCCTTGCTCTTCCTGCAATAACTTCAATTATTGAAGCTTATCCTTTCTAA

Protein sequence

EMEPKEANEAEAMLRGQADIWRYMLCFADSMALKSAVQLRLADIIKSHGSPLTLSQIAASIASSSASPNISFLARIMALLVRRNIFAAHHPSDGGETLYGLTHSSRWLLRDSDLTLAPMILAELHPWLLDPWHRFAQGVREGGNLFHKANGLHFWDFAARNPQFNRLFNDAMASTSKVVINAILSVYKDGFNSVGSLADVGGGIGGAVSEIVKAYPHMKGINYDLPHVVSTAPAYPGVTHVGGDMFESVPKADAIFMKWILHDWDDKECVRILENCKKAIPEKTGKVIIVEVVLNPEGEGPFDDTGLYFDLLMLVHSNGKERTEKEWKKILEEAGFLRYKLLALPAITSIIEAYPF

Homology

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BLAST of MC02g0479 vs. ExPASy Swiss-Prot
Match: B0ZB56 (Xanthohumol 4-O-methyltransferase OS=Humulus lupulus OX=3486 GN=OMT2 PE=1 SV=1)

HSP 1 Score: 401.7 bits (1031), Expect = 8.4e-111
Identity = 197/360 (54.72%), Postives = 265/360 (73.61%), Query Frame = 0


Query: 2   MEPKEANEAEAMLRGQADIWRYMLCFADSMALKSAVQLRLADIIKSHGSPLTLSQIAASI 61
           ME    ++ EA LRG+A++W+ +   AD M +K A++LR+ DI+ SH +P+TL+QIA+S+
Sbjct: 1   MELARNDQTEAALRGEANVWKSINGIADFMVMKCALELRIPDIVHSHSAPITLAQIASSV 60

Query: 62  ASSSASPNISFLARIMALLVRRNIFAAHHPSDGGETLYGLTHSSRWLLRDSDL----TLA 121
              S S N+S+L+RIM LLVRR IF+ H   DG E LYG THSSR LL  + L    TLA
Sbjct: 61  -PDSPSLNLSYLSRIMRLLVRRKIFSQHKSLDGEEVLYGPTHSSRLLLSKTTLPDQVTLA 120

Query: 122 PMILAELHPWLLDPWHRFAQGVREGGNLFHKAN-GLHFWDFAARNPQFNRLFNDAMASTS 181
           P +    HP+L  PW   A+ V+EGGN F   + G   WD +  NP+FN++FND MAST+
Sbjct: 121 PFVAFMTHPYLSAPWSCLARCVKEGGNGFEMVHGGRQLWDLSPGNPEFNKVFNDGMASTA 180

Query: 182 KVVINAILSVYKDGFNSVGSLADVGGGIGGAVSEIVKAYPHMKGINYDLPHVVSTAPAYP 241
           ++   AILS Y+D F  + SL DVGG  GG++S IVK++PH+KGINYDLPHVV+TAP Y 
Sbjct: 181 RITTMAILSEYRDVFCGICSLVDVGGEFGGSISAIVKSHPHIKGINYDLPHVVATAPTYT 240

Query: 242 G-VTHVGGDMFESVPKADAIFMKWILHDWDDKECVRILENCKKAIPEKTGKVIIVEVVLN 301
           G V+HVGG+MFE +P A A+FMKWILHDW D++CV+IL+NC++A+PEK GK+IIV++VL 
Sbjct: 241 GLVSHVGGNMFEWIPTAVAVFMKWILHDWADEDCVKILKNCRRAMPEKGGKIIIVDIVLE 300

Query: 302 PEGEGPFDDTGLYFDLLMLVHSNGKERTEKEWKKILEEAGFLRYKLLALPAITSIIEAYP 356
           PEG G FDD  +  D+ ++  + GKERTEKEWK++LEE GF RY++L +PA+TS+IEAYP
Sbjct: 301 PEGNGLFDDAAVMLDIALMALTRGKERTEKEWKRVLEEGGFPRYQILKIPALTSVIEAYP 359

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BLAST of MC02g0479 vs. ExPASy Swiss-Prot
Match: B0ZB55 (Desmethylxanthohumol 6'-O-methyltransferase OS=Humulus lupulus OX=3486 GN=OMT1 PE=1 SV=2)

HSP 1 Score: 382.1 bits (980), Expect = 6.9e-105
Identity = 187/347 (53.89%), Postives = 253/347 (72.91%), Query Frame = 0


Query: 14  LRGQADIWRYMLCFADSMALKSAVQLRLADIIKSHGSPLTLSQIAASIAS---SSASPNI 73
           LRGQ  IW+ M  F DSMALK A++LR+ADII SHG P+TLSQIA+ I S   SS SPNI
Sbjct: 4   LRGQEQIWQLMFSFVDSMALKCAIELRIADIIHSHGKPITLSQIASGIRSNSNSSISPNI 63

Query: 74  SFLARIMALLVRRNIFAAH-HPSDGGETLYGLTHSSRWLLRDSDLTLAPMILAELHPWLL 133
            +L+RIM  LVR+NIF  H   +D   +LYGL+ SSRWLLRD   +LAPM+L + HP  +
Sbjct: 64  PYLSRIMRFLVRKNIFTEHQEDNDEVISLYGLSDSSRWLLRDFKSSLAPMVLMQTHPLSM 123

Query: 134 DPWHRFAQGVREGGNLFHKANGLHFWDFAARNPQFNRLFNDAMASTSKVVINAILSVYKD 193
             WH     VR   N F KA+G + W+F++ NP FN++FN+AMAS   + + A+LS YKD
Sbjct: 124 AVWHFLEDYVRNSSNTFEKAHGCNIWEFSSANPDFNKIFNNAMASIVPIYMGAVLSSYKD 183

Query: 194 GFNSV-GSLADVGGGIGGAVSEIVKAYPHMKGINYDLPHVVSTAPAYPGVTHVGGDMFES 253
           G   + G++ DVGGG GG++SE++K YP++KGIN+DLPHV++TAPA  GVTH+ GD+FES
Sbjct: 184 GLGCIKGTVVDVGGGTGGSISELMKYYPNIKGINFDLPHVIATAPALDGVTHISGDIFES 243

Query: 254 VPKADAIFMKWILHDWDDKECVRILENCKKAIPE-KTGKVIIVEVVLNPEGEGPFDDTGL 313
           +P ADA+ MK +LH + D++CV++L NC+KAI + K GK+II+E+VL+P     FD+T +
Sbjct: 244 IPSADAVLMKGVLHCFSDEKCVKVLRNCRKAITDKKNGKIIILEIVLDPTSNQIFDETRM 303

Query: 314 YFDLLMLVHSNGKERTEKEWKKILEEAGFLRYKLLALPAITSIIEAY 355
            +DLL+ + S GKERTE EWK++L EAGF   K+  +P I +IIEA+
Sbjct: 304 VYDLLIPLFSGGKERTELEWKRLLNEAGFTSIKITKIPIIPAIIEAF 350

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BLAST of MC02g0479 vs. ExPASy Swiss-Prot
Match: Q6WUC2 ((R,S)-reticuline 7-O-methyltransferase OS=Papaver somniferum OX=3469 GN=7OMT PE=1 SV=1)

HSP 1 Score: 375.2 bits (962), Expect = 8.4e-103
Identity = 183/351 (52.14%), Postives = 245/351 (69.80%), Query Frame = 0


Query: 10  AEAMLRGQADIWRYMLCFADSMALKSAVQLRLADIIKSHGSPLTLSQIAASIASS--SAS 69
           AE  L+GQA+IW +M  F DSMALK AV+L + DII SHG P+T+S+I  S+ ++  S+S
Sbjct: 4   AEERLKGQAEIWEHMFAFVDSMALKCAVELGIPDIINSHGRPVTISEIVDSLKTNTPSSS 63

Query: 70  PNISFLARIMALLVRRNIFAAHHPSDGGETLYGLTHSSRWLLRDSDLTLAPMILAELHPW 129
           PNI +L RIM LLV + +F +    +  + LY LT SS+WLL+DS   L+P++L E +P 
Sbjct: 64  PNIDYLTRIMRLLVHKRLFTSELHQESNQLLYNLTRSSKWLLKDSKFNLSPLVLWETNPI 123

Query: 130 LLDPWHRFAQGVREGGNLFHKANGLHFWDFAARNPQFNRLFNDAMASTSKVVINAILSVY 189
           LL PW    +  +E  + F +A+G   WD A  +P+FN   N AM  ++  +IN +L  Y
Sbjct: 124 LLKPWQYLGKCAQEKSSPFERAHGCEIWDLALADPKFNNFLNGAMQCSTTTIINEMLLEY 183

Query: 190 KDGFNSV-GSLADVGGGIGGAVSEIVKAYPHMKGINYDLPHVVSTAPAYPGVTHVGGDMF 249
           KDGF+ + GSL DVGGG G  ++EIVKA+PH++GIN+DLPHVV+TA  +PGV HVGGDMF
Sbjct: 184 KDGFSGIAGSLVDVGGGTGSIIAEIVKAHPHIQGINFDLPHVVATAAEFPGVKHVGGDMF 243

Query: 250 ESVPKADAIFMKWILHDWDDKECVRILENCKKAI-PEKTGKVIIVEVVLNPEGEGPFDDT 309
             +P+ADA+ MKWILHDW D++C  IL+NC +AI  +K GKVIIV+ VL P+G   FD  
Sbjct: 244 VDIPEADAVIMKWILHDWSDEDCTIILKNCYRAIRKKKNGKVIIVDCVLRPDGNDLFDKM 303

Query: 310 GLYFDLLMLVHSN-GKERTEKEWKKILEEAGFLRYKLLALPAITSIIEAYP 356
           GL FD+LM+ H+  GKERTE EWK +L  AGF RY ++  PA   IIEA+P
Sbjct: 304 GLIFDVLMMAHTTAGKERTEAEWKILLNNAGFPRYNVIRTPAFPCIIEAFP 354

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BLAST of MC02g0479 vs. ExPASy Swiss-Prot
Match: Q9LEL6 ((RS)-norcoclaurine 6-O-methyltransferase OS=Coptis japonica OX=3442 PE=1 SV=1)

HSP 1 Score: 298.5 bits (763), Expect = 1.0e-79
Identity = 156/356 (43.82%), Postives = 228/356 (64.04%), Query Frame = 0


Query: 2   MEPKEANEAEAMLRGQADIWRYMLCFADSMALKSAVQLRLADIIKSHGSPLTLSQIAASI 61
           ME K+ N     L  QA +W ++  FA+S+ LK AVQL LA+II + G+ +TLS++++ +
Sbjct: 1   MEVKKDN-----LSSQAKLWNFIYGFAESLVLKCAVQLDLANIIHNSGTSMTLSELSSRL 60

Query: 62  ASSSASPNISFLARIMALLVRRNIFAAHHPSDGGETLYGLTHSSRWLLRDSDLTLAPMIL 121
            S     N   L R+M  LV   +F     S  GE  YGL   +++L++  D  +   IL
Sbjct: 61  PSQPV--NEDALYRVMRYLVHMKLFT--KASIDGELRYGLAPPAKYLVKGWDKCMVGSIL 120

Query: 122 AELHPWLLDPWHRFAQGVR-EGGNLFHKANGLHFWDFAARNPQFNRLFNDAMASTSKVVI 181
           A      + PWH    G+  E G  F KA G + W + A +P+ N+LFN+AMA+ S++++
Sbjct: 121 AITDKDFMAPWHYLKDGLSGESGTAFEKALGTNIWGYMAEHPEKNQLFNEAMANDSRLIM 180

Query: 182 NAILSVYKDGFNSVGSLADVGGGIGGAVSEIVKAYPHMKGINYDLPHVVSTAPAYPGVTH 241
           +A++    + FN + +L DVGGG G AV  I  A+PH+K   YDLPHV++ +P Y  V  
Sbjct: 181 SALVKECGNIFNGITTLVDVGGGTGTAVRNIANAFPHIKCTVYDLPHVIADSPGYSEVHC 240

Query: 242 VGGDMFESVPKADAIFMKWILHDWDDKECVRILENCKKAIPEKTGKVIIVEVVLNPEGEG 301
           V GDMF+ +PKADAI MK ILHDWDDKEC+ IL+ CK+A+P K GKVIIV++VLN + E 
Sbjct: 241 VAGDMFKFIPKADAIMMKCILHDWDDKECIEILKRCKEAVPVKGGKVIIVDIVLNVQSEH 300

Query: 302 PFDDTGLYFDLLMLVHSNGKERTEKEWKKILEEAGFLRYKLLALPAITSIIEAYPF 357
           P+    L  DL M++++ GKERTE+EWKK++ +AG+  +K+  + A+ S+IEAYP+
Sbjct: 301 PYTKMRLTLDLDMMLNTGGKERTEEEWKKLIHDAGYKGHKITQITAVQSVIEAYPY 347

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BLAST of MC02g0479 vs. ExPASy Swiss-Prot
Match: Q5C9L7 ((RS)-norcoclaurine 6-O-methyltransferase OS=Thalictrum flavum subsp. glaucum OX=150095 PE=1 SV=1)

HSP 1 Score: 297.4 bits (760), Expect = 2.2e-79
Identity = 146/346 (42.20%), Postives = 222/346 (64.16%), Query Frame = 0


Query: 14  LRGQADIWRYMLCFADSMALKSAVQLRLADIIKSHGSPLTLSQIAASIASSSASPNISFL 73
           L  QA +W ++  FADS+ LKSAVQL LA+II +HGSP+TLS+++  + S     N   L
Sbjct: 9   LSSQAKLWNFIYGFADSLVLKSAVQLDLANIIHNHGSPMTLSELSLHLPSQPV--NQDAL 68

Query: 74  ARIMALLVRRNIFAAHHPSDGGETLYGLTHSSRWLLRDSDLTLAPMILAELHPWLLDPWH 133
            R++  LV   +F     S  GE  YGL   +++L++  D  +   IL       + PWH
Sbjct: 69  YRVLRYLVHMKLFT--KSSIDGELRYGLAPPAKFLVKGWDKCMLGAILTITDKDFMAPWH 128

Query: 134 RFAQGVREGGN---LFHKANGLHFWDFAARNPQFNRLFNDAMASTSKVVINAILSVYKDG 193
              +G+   G+    F KA G + WD+ A +P+ N+LFN+ MA+ ++++++A++      
Sbjct: 129 YLKEGILNDGSTSTAFEKALGTNIWDYMAEHPEKNQLFNEGMANDTRLIMSALVKECSSM 188

Query: 194 FNSVGSLADVGGGIGGAVSEIVKAYPHMKGINYDLPHVVSTAPAYPGVTHVGGDMFESVP 253
           F+ + ++ DVGGG G AV  I KA+PH+K   YDLPHV++ +P Y  +  + GDMF+ +P
Sbjct: 189 FDGITTIVDVGGGTGTAVRNIAKAFPHIKCTVYDLPHVIADSPGYTEINSIQGDMFKYIP 248

Query: 254 KADAIFMKWILHDWDDKECVRILENCKKAIPEKTGKVIIVEVVLNPEGEGPFDDTGLYFD 313
            ADAI MK ILHDWDDKEC+ IL+ CK A+P   GKVII++++L+ + E P+    L  D
Sbjct: 249 NADAIMMKCILHDWDDKECIEILKRCKDAVPRDGGKVIIIDIILDVKSEHPYTKMRLTLD 308

Query: 314 LLMLVHSNGKERTEKEWKKILEEAGFLRYKLLALPAITSIIEAYPF 357
           L M++++ GKERTE+EWKK++ +AG+  YK+  + A+ S+IEAYP+
Sbjct: 309 LDMMLNTGGKERTEEEWKKLIHDAGYKGYKITHISAVQSVIEAYPY 350

[Close]

BLAST of MC02g0479 vs. NCBI nr
Match: XP_022140297.1 (xanthohumol 4'-O-methyltransferase-like [Momordica charantia])

HSP 1 Score: 716 bits (1848), Expect = 2.13e-260
Identity = 355/355 (100.00%), Postives = 355/355 (100.00%), Query Frame = 0


Query: 2   MEPKEANEAEAMLRGQADIWRYMLCFADSMALKSAVQLRLADIIKSHGSPLTLSQIAASI 61
           MEPKEANEAEAMLRGQADIWRYMLCFADSMALKSAVQLRLADIIKSHGSPLTLSQIAASI
Sbjct: 1   MEPKEANEAEAMLRGQADIWRYMLCFADSMALKSAVQLRLADIIKSHGSPLTLSQIAASI 60

Query: 62  ASSSASPNISFLARIMALLVRRNIFAAHHPSDGGETLYGLTHSSRWLLRDSDLTLAPMIL 121
           ASSSASPNISFLARIMALLVRRNIFAAHHPSDGGETLYGLTHSSRWLLRDSDLTLAPMIL
Sbjct: 61  ASSSASPNISFLARIMALLVRRNIFAAHHPSDGGETLYGLTHSSRWLLRDSDLTLAPMIL 120

Query: 122 AELHPWLLDPWHRFAQGVREGGNLFHKANGLHFWDFAARNPQFNRLFNDAMASTSKVVIN 181
           AELHPWLLDPWHRFAQGVREGGNLFHKANGLHFWDFAARNPQFNRLFNDAMASTSKVVIN
Sbjct: 121 AELHPWLLDPWHRFAQGVREGGNLFHKANGLHFWDFAARNPQFNRLFNDAMASTSKVVIN 180

Query: 182 AILSVYKDGFNSVGSLADVGGGIGGAVSEIVKAYPHMKGINYDLPHVVSTAPAYPGVTHV 241
           AILSVYKDGFNSVGSLADVGGGIGGAVSEIVKAYPHMKGINYDLPHVVSTAPAYPGVTHV
Sbjct: 181 AILSVYKDGFNSVGSLADVGGGIGGAVSEIVKAYPHMKGINYDLPHVVSTAPAYPGVTHV 240

Query: 242 GGDMFESVPKADAIFMKWILHDWDDKECVRILENCKKAIPEKTGKVIIVEVVLNPEGEGP 301
           GGDMFESVPKADAIFMKWILHDWDDKECVRILENCKKAIPEKTGKVIIVEVVLNPEGEGP
Sbjct: 241 GGDMFESVPKADAIFMKWILHDWDDKECVRILENCKKAIPEKTGKVIIVEVVLNPEGEGP 300

Query: 302 FDDTGLYFDLLMLVHSNGKERTEKEWKKILEEAGFLRYKLLALPAITSIIEAYPF 356
           FDDTGLYFDLLMLVHSNGKERTEKEWKKILEEAGFLRYKLLALPAITSIIEAYPF
Sbjct: 301 FDDTGLYFDLLMLVHSNGKERTEKEWKKILEEAGFLRYKLLALPAITSIIEAYPF 355

[Close]

BLAST of MC02g0479 vs. NCBI nr
Match: XP_022140296.1 (xanthohumol 4'-O-methyltransferase-like [Momordica charantia])

HSP 1 Score: 645 bits (1664), Expect = 2.36e-232
Identity = 318/354 (89.83%), Postives = 335/354 (94.63%), Query Frame = 0


Query: 2   MEPKEANEAEAMLRGQADIWRYMLCFADSMALKSAVQLRLADIIKSHGSPLTLSQIAASI 61
           ME KEA E EA LRGQADIWRYMLCFADSMALKSAVQL+LADII SHGSP+TLSQIA+SI
Sbjct: 1   MEAKEAKETEAELRGQADIWRYMLCFADSMALKSAVQLQLADIINSHGSPITLSQIASSI 60

Query: 62  ASSSASPNISFLARIMALLVRRNIFAAHHPSDGGETLYGLTHSSRWLLRDSDLTLAPMIL 121
           ASSSASPNIS+LARIM LLVRRNIFAAHHPSDGG+TLYGLTHSSRWLLRDSDLTLAPMIL
Sbjct: 61  ASSSASPNISYLARIMRLLVRRNIFAAHHPSDGGDTLYGLTHSSRWLLRDSDLTLAPMIL 120

Query: 122 AELHPWLLDPWHRFAQGVREGGNLFHKANGLHFWDFAARNPQFNRLFNDAMASTSKVVIN 181
           AELH WL+DPWH FA+GV+EGG++F KANG   W FA++NPQFN+LFNDAMASTSKVVIN
Sbjct: 121 AELHHWLVDPWHCFAEGVKEGGDIFQKANGHDIWGFASKNPQFNQLFNDAMASTSKVVIN 180

Query: 182 AILSVYKDGFNSVGSLADVGGGIGGAVSEIVKAYPHMKGINYDLPHVVSTAPAYPGVTHV 241
           AILSVYKDGFNSVGSLADVGGGIGGAVSEIVKAYPHMKGINYDLPHVVSTA AYPGVTHV
Sbjct: 181 AILSVYKDGFNSVGSLADVGGGIGGAVSEIVKAYPHMKGINYDLPHVVSTATAYPGVTHV 240

Query: 242 GGDMFESVPKADAIFMKWILHDWDDKECVRILENCKKAIPEKTGKVIIVEVVLNPEGEGP 301
           GGDMFESVPKADAIFMKWILHDWDDKECVRILENC+KAIPEKTGKVIIVEVVLNPEGEG 
Sbjct: 241 GGDMFESVPKADAIFMKWILHDWDDKECVRILENCRKAIPEKTGKVIIVEVVLNPEGEGA 300

Query: 302 FDDTGLYFDLLMLVHSNGKERTEKEWKKILEEAGFLRYKLLALPAITSIIEAYP 355
           FDDT LYFDLLML H+NGKERTEKEWKKILEEAGF RYKL+ LPA+TSIIEAYP
Sbjct: 301 FDDTRLYFDLLMLAHTNGKERTEKEWKKILEEAGFRRYKLVTLPALTSIIEAYP 354

[Close]

BLAST of MC02g0479 vs. NCBI nr
Match: XP_008448276.2 (PREDICTED: (R,S)-reticuline 7-O-methyltransferase-like [Cucumis melo] >KAA0062101.1 (R,S)-reticuline 7-O-methyltransferase-like [Cucumis melo var. makuwa] >TYK04926.1 (R,S)-reticuline 7-O-methyltransferase-like [Cucumis melo var. makuwa])

HSP 1 Score: 570 bits (1469), Expect = 1.28e-202
Identity = 275/356 (77.25%), Postives = 314/356 (88.20%), Query Frame = 0


Query: 2   MEPKEANEAEAMLRGQADIWRYMLCFADSMALKSAVQLRLADIIKSHGSPLTLSQIAASI 61
           ME KE NEAEA+L+GQA+IW+YMLCFADSMALK AV+L LADII SHGSP++LSQI++SI
Sbjct: 1   MEAKETNEAEALLQGQAEIWKYMLCFADSMALKCAVELHLADIINSHGSPISLSQISSSI 60

Query: 62  ASS--SASPNISFLARIMALLVRRNIFAAHHPSDGGETLYGLTHSSRWLLRDSDLTLAPM 121
           A+S  S+SP IS+L RIM LLVRRNIFAAHHPSDGG+TLYGLTHSS+WLLRDS LTLAPM
Sbjct: 61  AASNPSSSPQISYLNRIMRLLVRRNIFAAHHPSDGGDTLYGLTHSSKWLLRDSPLTLAPM 120

Query: 122 ILAELHPWLLDPWHRFAQGVREGGNLFHKANGLHFWDFAARNPQFNRLFNDAMASTSKVV 181
             +ELH W++ PW  F + V+EGG+ F  A+GL  WDFA++NPQFN  FNDAMASTSKVV
Sbjct: 121 AFSELHQWMVSPWLCFTEAVKEGGSPFKIAHGLDIWDFASKNPQFNHFFNDAMASTSKVV 180

Query: 182 INAILSVYKDGFNSVGSLADVGGGIGGAVSEIVKAYPHMKGINYDLPHVVSTAPAYPGVT 241
           +NAILSVY+DGFNS+ SLADVGGGIGG++SEIVKA+PH+KGINYDLPHV+STAP Y GVT
Sbjct: 181 MNAILSVYQDGFNSLDSLADVGGGIGGSISEIVKAFPHIKGINYDLPHVISTAPVYEGVT 240

Query: 242 HVGGDMFESVPKADAIFMKWILHDWDDKECVRILENCKKAIPEKTGKVIIVEVVLNPEGE 301
           H+GGDMFE +PK DAIFMKWILHDW+DKECV+ILENCKKAIPEK GKVIIVEVVLN EG+
Sbjct: 241 HIGGDMFEDIPKVDAIFMKWILHDWNDKECVKILENCKKAIPEKRGKVIIVEVVLNEEGK 300

Query: 302 GPFDDTGLYFDLLMLVHSNGKERTEKEWKKILEEAGFLRYKLLALPAITSIIEAYP 355
           G FDDT  YFDLLML H+NGKERTEKEWK ILEEAGF RY L+ LPA+ SIIEAYP
Sbjct: 301 GAFDDTKFYFDLLMLAHTNGKERTEKEWKTILEEAGFSRYNLIPLPALVSIIEAYP 356

[Close]

BLAST of MC02g0479 vs. NCBI nr
Match: XP_038902222.1 (xanthohumol 4-O-methyltransferase-like [Benincasa hispida])

HSP 1 Score: 568 bits (1464), Expect = 7.41e-202
Identity = 275/356 (77.25%), Postives = 314/356 (88.20%), Query Frame = 0


Query: 2   MEPKEANEAEAMLRGQADIWRYMLCFADSMALKSAVQLRLADIIKSHGSPLTLSQIAASI 61
           ME KE  E E +L+GQ+ IW+YMLCFADSMALK AV+L L DII SHGSP++LSQIA+SI
Sbjct: 1   MEAKETKETEDLLKGQSVIWKYMLCFADSMALKCAVELHLPDIINSHGSPISLSQIASSI 60

Query: 62  ASS--SASPNISFLARIMALLVRRNIFAAHHPSDGGETLYGLTHSSRWLLRDSDLTLAPM 121
           A+S  S+SP IS+L RIM LLVRRNIF AHHPS+GGETLYGLTHSS+WLLRDS L+LAPM
Sbjct: 61  AASNPSSSPQISYLTRIMRLLVRRNIFTAHHPSNGGETLYGLTHSSKWLLRDSPLSLAPM 120

Query: 122 ILAELHPWLLDPWHRFAQGVREGGNLFHKANGLHFWDFAARNPQFNRLFNDAMASTSKVV 181
           + +ELH W+++PW  F Q V+EGG+ F  ANGL  WDFA++NPQFN  FNDAMASTSKVV
Sbjct: 121 VFSELHQWMVNPWLCFTQAVKEGGSPFQIANGLDIWDFASKNPQFNHFFNDAMASTSKVV 180

Query: 182 INAILSVYKDGFNSVGSLADVGGGIGGAVSEIVKAYPHMKGINYDLPHVVSTAPAYPGVT 241
           +NAILSVYKDGFNSV SLADVGGGIGG+VSEIVKA+PH+KGINYDLPHV+STAP Y GVT
Sbjct: 181 MNAILSVYKDGFNSVQSLADVGGGIGGSVSEIVKAFPHVKGINYDLPHVISTAPLYHGVT 240

Query: 242 HVGGDMFESVPKADAIFMKWILHDWDDKECVRILENCKKAIPEKTGKVIIVEVVLNPEGE 301
           H+GGDMF+ +PKADAIFMKWILHDW+DKECV+ILENCKKAIPEKTGKVIIVEVVLN +GE
Sbjct: 241 HIGGDMFDYIPKADAIFMKWILHDWNDKECVKILENCKKAIPEKTGKVIIVEVVLNEDGE 300

Query: 302 GPFDDTGLYFDLLMLVHSNGKERTEKEWKKILEEAGFLRYKLLALPAITSIIEAYP 355
           G FDDT  YFDLLML H+NGKERTEKEWK ILEEAGF RY+++ LPA+TSIIEAYP
Sbjct: 301 GTFDDTRFYFDLLMLAHTNGKERTEKEWKTILEEAGFPRYRVIPLPALTSIIEAYP 356

[Close]

BLAST of MC02g0479 vs. NCBI nr
Match: XP_022970718.1 (xanthohumol 4'-O-methyltransferase-like [Cucurbita maxima])

HSP 1 Score: 565 bits (1457), Expect = 8.63e-201
Identity = 267/356 (75.00%), Postives = 313/356 (87.92%), Query Frame = 0


Query: 2   MEPKEANEAEAMLRGQADIWRYMLCFADSMALKSAVQLRLADIIKSHGSPLTLSQIAASI 61
           M  KE  E E +L+GQA+IW+YMLCFADSMALK AV+L LADII SH SPL+LSQIA+SI
Sbjct: 1   MATKETEETEELLKGQAEIWKYMLCFADSMALKCAVELHLADIINSHASPLSLSQIASSI 60

Query: 62  ASSS--ASPNISFLARIMALLVRRNIFAAHHPSDGGETLYGLTHSSRWLLRDSDLTLAPM 121
            +S   A+P IS+L RIM LLVRRNIFA HH  DGGE LYGLTHSS+WLLRD++ TLAPM
Sbjct: 61  VASDPLATPEISYLTRIMRLLVRRNIFAVHHSPDGGEPLYGLTHSSKWLLRDAEHTLAPM 120

Query: 122 ILAELHPWLLDPWHRFAQGVREGGNLFHKANGLHFWDFAARNPQFNRLFNDAMASTSKVV 181
           +LAELH W++DPW  F+QGV+EGG+ F KANGL  W FA+RNPQFN+LFN+AMASTSKV+
Sbjct: 121 VLAELHKWMVDPWLCFSQGVKEGGDQFQKANGLDIWSFASRNPQFNQLFNNAMASTSKVI 180

Query: 182 INAILSVYKDGFNSVGSLADVGGGIGGAVSEIVKAYPHMKGINYDLPHVVSTAPAYPGVT 241
           + A+LSVY+DGF+S+ SL DVGGGIGG +SEIVKA+PH+KGINYDLPHV+STAP Y GVT
Sbjct: 181 MKAVLSVYQDGFSSIESLTDVGGGIGGVISEIVKAFPHIKGINYDLPHVISTAPPYQGVT 240

Query: 242 HVGGDMFESVPKADAIFMKWILHDWDDKECVRILENCKKAIPEKTGKVIIVEVVLNPEGE 301
           H+GGDMFE++PKADA+FMKWILHDWDDKECVRILENCKKAIPEKTGKVI+VEV+L  +G+
Sbjct: 241 HIGGDMFEAIPKADAVFMKWILHDWDDKECVRILENCKKAIPEKTGKVILVEVILKADGD 300

Query: 302 GPFDDTGLYFDLLMLVHSNGKERTEKEWKKILEEAGFLRYKLLALPAITSIIEAYP 355
           GPFDDT  YFDLLML H+NG+ERTEKEWK ILEEAGF RY+++ALPA+TSIIEAYP
Sbjct: 301 GPFDDTRFYFDLLMLAHTNGRERTEKEWKNILEEAGFPRYRVIALPALTSIIEAYP 356

[Close]

BLAST of MC02g0479 vs. ExPASy TrEMBL
Match: A0A6J1CGG0 (xanthohumol 4'-O-methyltransferase-like OS=Momordica charantia OX=3673 GN=LOC111011000 PE=3 SV=1)

HSP 1 Score: 716 bits (1848), Expect = 1.03e-260
Identity = 355/355 (100.00%), Postives = 355/355 (100.00%), Query Frame = 0


Query: 2   MEPKEANEAEAMLRGQADIWRYMLCFADSMALKSAVQLRLADIIKSHGSPLTLSQIAASI 61
           MEPKEANEAEAMLRGQADIWRYMLCFADSMALKSAVQLRLADIIKSHGSPLTLSQIAASI
Sbjct: 1   MEPKEANEAEAMLRGQADIWRYMLCFADSMALKSAVQLRLADIIKSHGSPLTLSQIAASI 60

Query: 62  ASSSASPNISFLARIMALLVRRNIFAAHHPSDGGETLYGLTHSSRWLLRDSDLTLAPMIL 121
           ASSSASPNISFLARIMALLVRRNIFAAHHPSDGGETLYGLTHSSRWLLRDSDLTLAPMIL
Sbjct: 61  ASSSASPNISFLARIMALLVRRNIFAAHHPSDGGETLYGLTHSSRWLLRDSDLTLAPMIL 120

Query: 122 AELHPWLLDPWHRFAQGVREGGNLFHKANGLHFWDFAARNPQFNRLFNDAMASTSKVVIN 181
           AELHPWLLDPWHRFAQGVREGGNLFHKANGLHFWDFAARNPQFNRLFNDAMASTSKVVIN
Sbjct: 121 AELHPWLLDPWHRFAQGVREGGNLFHKANGLHFWDFAARNPQFNRLFNDAMASTSKVVIN 180

Query: 182 AILSVYKDGFNSVGSLADVGGGIGGAVSEIVKAYPHMKGINYDLPHVVSTAPAYPGVTHV 241
           AILSVYKDGFNSVGSLADVGGGIGGAVSEIVKAYPHMKGINYDLPHVVSTAPAYPGVTHV
Sbjct: 181 AILSVYKDGFNSVGSLADVGGGIGGAVSEIVKAYPHMKGINYDLPHVVSTAPAYPGVTHV 240

Query: 242 GGDMFESVPKADAIFMKWILHDWDDKECVRILENCKKAIPEKTGKVIIVEVVLNPEGEGP 301
           GGDMFESVPKADAIFMKWILHDWDDKECVRILENCKKAIPEKTGKVIIVEVVLNPEGEGP
Sbjct: 241 GGDMFESVPKADAIFMKWILHDWDDKECVRILENCKKAIPEKTGKVIIVEVVLNPEGEGP 300

Query: 302 FDDTGLYFDLLMLVHSNGKERTEKEWKKILEEAGFLRYKLLALPAITSIIEAYPF 356
           FDDTGLYFDLLMLVHSNGKERTEKEWKKILEEAGFLRYKLLALPAITSIIEAYPF
Sbjct: 301 FDDTGLYFDLLMLVHSNGKERTEKEWKKILEEAGFLRYKLLALPAITSIIEAYPF 355

[Close]

BLAST of MC02g0479 vs. ExPASy TrEMBL
Match: A0A6J1CFN9 (xanthohumol 4'-O-methyltransferase-like OS=Momordica charantia OX=3673 GN=LOC111010999 PE=3 SV=1)

HSP 1 Score: 645 bits (1664), Expect = 1.14e-232
Identity = 318/354 (89.83%), Postives = 335/354 (94.63%), Query Frame = 0


Query: 2   MEPKEANEAEAMLRGQADIWRYMLCFADSMALKSAVQLRLADIIKSHGSPLTLSQIAASI 61
           ME KEA E EA LRGQADIWRYMLCFADSMALKSAVQL+LADII SHGSP+TLSQIA+SI
Sbjct: 1   MEAKEAKETEAELRGQADIWRYMLCFADSMALKSAVQLQLADIINSHGSPITLSQIASSI 60

Query: 62  ASSSASPNISFLARIMALLVRRNIFAAHHPSDGGETLYGLTHSSRWLLRDSDLTLAPMIL 121
           ASSSASPNIS+LARIM LLVRRNIFAAHHPSDGG+TLYGLTHSSRWLLRDSDLTLAPMIL
Sbjct: 61  ASSSASPNISYLARIMRLLVRRNIFAAHHPSDGGDTLYGLTHSSRWLLRDSDLTLAPMIL 120

Query: 122 AELHPWLLDPWHRFAQGVREGGNLFHKANGLHFWDFAARNPQFNRLFNDAMASTSKVVIN 181
           AELH WL+DPWH FA+GV+EGG++F KANG   W FA++NPQFN+LFNDAMASTSKVVIN
Sbjct: 121 AELHHWLVDPWHCFAEGVKEGGDIFQKANGHDIWGFASKNPQFNQLFNDAMASTSKVVIN 180

Query: 182 AILSVYKDGFNSVGSLADVGGGIGGAVSEIVKAYPHMKGINYDLPHVVSTAPAYPGVTHV 241
           AILSVYKDGFNSVGSLADVGGGIGGAVSEIVKAYPHMKGINYDLPHVVSTA AYPGVTHV
Sbjct: 181 AILSVYKDGFNSVGSLADVGGGIGGAVSEIVKAYPHMKGINYDLPHVVSTATAYPGVTHV 240

Query: 242 GGDMFESVPKADAIFMKWILHDWDDKECVRILENCKKAIPEKTGKVIIVEVVLNPEGEGP 301
           GGDMFESVPKADAIFMKWILHDWDDKECVRILENC+KAIPEKTGKVIIVEVVLNPEGEG 
Sbjct: 241 GGDMFESVPKADAIFMKWILHDWDDKECVRILENCRKAIPEKTGKVIIVEVVLNPEGEGA 300

Query: 302 FDDTGLYFDLLMLVHSNGKERTEKEWKKILEEAGFLRYKLLALPAITSIIEAYP 355
           FDDT LYFDLLML H+NGKERTEKEWKKILEEAGF RYKL+ LPA+TSIIEAYP
Sbjct: 301 FDDTRLYFDLLMLAHTNGKERTEKEWKKILEEAGFRRYKLVTLPALTSIIEAYP 354

[Close]

BLAST of MC02g0479 vs. ExPASy TrEMBL
Match: A0A5A7V8T5 ((R,S)-reticuline 7-O-methyltransferase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold143G001350 PE=3 SV=1)

HSP 1 Score: 570 bits (1469), Expect = 6.22e-203
Identity = 275/356 (77.25%), Postives = 314/356 (88.20%), Query Frame = 0


Query: 2   MEPKEANEAEAMLRGQADIWRYMLCFADSMALKSAVQLRLADIIKSHGSPLTLSQIAASI 61
           ME KE NEAEA+L+GQA+IW+YMLCFADSMALK AV+L LADII SHGSP++LSQI++SI
Sbjct: 1   MEAKETNEAEALLQGQAEIWKYMLCFADSMALKCAVELHLADIINSHGSPISLSQISSSI 60

Query: 62  ASS--SASPNISFLARIMALLVRRNIFAAHHPSDGGETLYGLTHSSRWLLRDSDLTLAPM 121
           A+S  S+SP IS+L RIM LLVRRNIFAAHHPSDGG+TLYGLTHSS+WLLRDS LTLAPM
Sbjct: 61  AASNPSSSPQISYLNRIMRLLVRRNIFAAHHPSDGGDTLYGLTHSSKWLLRDSPLTLAPM 120

Query: 122 ILAELHPWLLDPWHRFAQGVREGGNLFHKANGLHFWDFAARNPQFNRLFNDAMASTSKVV 181
             +ELH W++ PW  F + V+EGG+ F  A+GL  WDFA++NPQFN  FNDAMASTSKVV
Sbjct: 121 AFSELHQWMVSPWLCFTEAVKEGGSPFKIAHGLDIWDFASKNPQFNHFFNDAMASTSKVV 180

Query: 182 INAILSVYKDGFNSVGSLADVGGGIGGAVSEIVKAYPHMKGINYDLPHVVSTAPAYPGVT 241
           +NAILSVY+DGFNS+ SLADVGGGIGG++SEIVKA+PH+KGINYDLPHV+STAP Y GVT
Sbjct: 181 MNAILSVYQDGFNSLDSLADVGGGIGGSISEIVKAFPHIKGINYDLPHVISTAPVYEGVT 240

Query: 242 HVGGDMFESVPKADAIFMKWILHDWDDKECVRILENCKKAIPEKTGKVIIVEVVLNPEGE 301
           H+GGDMFE +PK DAIFMKWILHDW+DKECV+ILENCKKAIPEK GKVIIVEVVLN EG+
Sbjct: 241 HIGGDMFEDIPKVDAIFMKWILHDWNDKECVKILENCKKAIPEKRGKVIIVEVVLNEEGK 300

Query: 302 GPFDDTGLYFDLLMLVHSNGKERTEKEWKKILEEAGFLRYKLLALPAITSIIEAYP 355
           G FDDT  YFDLLML H+NGKERTEKEWK ILEEAGF RY L+ LPA+ SIIEAYP
Sbjct: 301 GAFDDTKFYFDLLMLAHTNGKERTEKEWKTILEEAGFSRYNLIPLPALVSIIEAYP 356

[Close]

BLAST of MC02g0479 vs. ExPASy TrEMBL
Match: A0A1S3BJA7 ((R,S)-reticuline 7-O-methyltransferase-like OS=Cucumis melo OX=3656 GN=LOC103490512 PE=3 SV=1)

HSP 1 Score: 570 bits (1469), Expect = 6.22e-203
Identity = 275/356 (77.25%), Postives = 314/356 (88.20%), Query Frame = 0


Query: 2   MEPKEANEAEAMLRGQADIWRYMLCFADSMALKSAVQLRLADIIKSHGSPLTLSQIAASI 61
           ME KE NEAEA+L+GQA+IW+YMLCFADSMALK AV+L LADII SHGSP++LSQI++SI
Sbjct: 1   MEAKETNEAEALLQGQAEIWKYMLCFADSMALKCAVELHLADIINSHGSPISLSQISSSI 60

Query: 62  ASS--SASPNISFLARIMALLVRRNIFAAHHPSDGGETLYGLTHSSRWLLRDSDLTLAPM 121
           A+S  S+SP IS+L RIM LLVRRNIFAAHHPSDGG+TLYGLTHSS+WLLRDS LTLAPM
Sbjct: 61  AASNPSSSPQISYLNRIMRLLVRRNIFAAHHPSDGGDTLYGLTHSSKWLLRDSPLTLAPM 120

Query: 122 ILAELHPWLLDPWHRFAQGVREGGNLFHKANGLHFWDFAARNPQFNRLFNDAMASTSKVV 181
             +ELH W++ PW  F + V+EGG+ F  A+GL  WDFA++NPQFN  FNDAMASTSKVV
Sbjct: 121 AFSELHQWMVSPWLCFTEAVKEGGSPFKIAHGLDIWDFASKNPQFNHFFNDAMASTSKVV 180

Query: 182 INAILSVYKDGFNSVGSLADVGGGIGGAVSEIVKAYPHMKGINYDLPHVVSTAPAYPGVT 241
           +NAILSVY+DGFNS+ SLADVGGGIGG++SEIVKA+PH+KGINYDLPHV+STAP Y GVT
Sbjct: 181 MNAILSVYQDGFNSLDSLADVGGGIGGSISEIVKAFPHIKGINYDLPHVISTAPVYEGVT 240

Query: 242 HVGGDMFESVPKADAIFMKWILHDWDDKECVRILENCKKAIPEKTGKVIIVEVVLNPEGE 301
           H+GGDMFE +PK DAIFMKWILHDW+DKECV+ILENCKKAIPEK GKVIIVEVVLN EG+
Sbjct: 241 HIGGDMFEDIPKVDAIFMKWILHDWNDKECVKILENCKKAIPEKRGKVIIVEVVLNEEGK 300

Query: 302 GPFDDTGLYFDLLMLVHSNGKERTEKEWKKILEEAGFLRYKLLALPAITSIIEAYP 355
           G FDDT  YFDLLML H+NGKERTEKEWK ILEEAGF RY L+ LPA+ SIIEAYP
Sbjct: 301 GAFDDTKFYFDLLMLAHTNGKERTEKEWKTILEEAGFSRYNLIPLPALVSIIEAYP 356

[Close]

BLAST of MC02g0479 vs. ExPASy TrEMBL
Match: A0A6J1I4R1 (xanthohumol 4'-O-methyltransferase-like OS=Cucurbita maxima OX=3661 GN=LOC111469616 PE=3 SV=1)

HSP 1 Score: 565 bits (1457), Expect = 4.18e-201
Identity = 267/356 (75.00%), Postives = 313/356 (87.92%), Query Frame = 0


Query: 2   MEPKEANEAEAMLRGQADIWRYMLCFADSMALKSAVQLRLADIIKSHGSPLTLSQIAASI 61
           M  KE  E E +L+GQA+IW+YMLCFADSMALK AV+L LADII SH SPL+LSQIA+SI
Sbjct: 1   MATKETEETEELLKGQAEIWKYMLCFADSMALKCAVELHLADIINSHASPLSLSQIASSI 60

Query: 62  ASSS--ASPNISFLARIMALLVRRNIFAAHHPSDGGETLYGLTHSSRWLLRDSDLTLAPM 121
            +S   A+P IS+L RIM LLVRRNIFA HH  DGGE LYGLTHSS+WLLRD++ TLAPM
Sbjct: 61  VASDPLATPEISYLTRIMRLLVRRNIFAVHHSPDGGEPLYGLTHSSKWLLRDAEHTLAPM 120

Query: 122 ILAELHPWLLDPWHRFAQGVREGGNLFHKANGLHFWDFAARNPQFNRLFNDAMASTSKVV 181
           +LAELH W++DPW  F+QGV+EGG+ F KANGL  W FA+RNPQFN+LFN+AMASTSKV+
Sbjct: 121 VLAELHKWMVDPWLCFSQGVKEGGDQFQKANGLDIWSFASRNPQFNQLFNNAMASTSKVI 180

Query: 182 INAILSVYKDGFNSVGSLADVGGGIGGAVSEIVKAYPHMKGINYDLPHVVSTAPAYPGVT 241
           + A+LSVY+DGF+S+ SL DVGGGIGG +SEIVKA+PH+KGINYDLPHV+STAP Y GVT
Sbjct: 181 MKAVLSVYQDGFSSIESLTDVGGGIGGVISEIVKAFPHIKGINYDLPHVISTAPPYQGVT 240

Query: 242 HVGGDMFESVPKADAIFMKWILHDWDDKECVRILENCKKAIPEKTGKVIIVEVVLNPEGE 301
           H+GGDMFE++PKADA+FMKWILHDWDDKECVRILENCKKAIPEKTGKVI+VEV+L  +G+
Sbjct: 241 HIGGDMFEAIPKADAVFMKWILHDWDDKECVRILENCKKAIPEKTGKVILVEVILKADGD 300

Query: 302 GPFDDTGLYFDLLMLVHSNGKERTEKEWKKILEEAGFLRYKLLALPAITSIIEAYP 355
           GPFDDT  YFDLLML H+NG+ERTEKEWK ILEEAGF RY+++ALPA+TSIIEAYP
Sbjct: 301 GPFDDTRFYFDLLMLAHTNGRERTEKEWKNILEEAGFPRYRVIALPALTSIIEAYP 356

[Close]

BLAST of MC02g0479 vs. TAIR 10
Match: AT4G35160.1 (O-methyltransferase family protein )

HSP 1 Score: 254.6 bits (649), Expect = 1.2e-67
Identity = 146/372 (39.25%), Postives = 214/372 (57.53%), Query Frame = 0


Query: 1   EMEPKEANEAEAMLRGQADIWRYMLCFADSMALKSAVQLRLADIIKSHGS--PLTLSQIA 60
           +ME  +    +   +   DIW+Y+  FAD  A K A+ L++ + I++H S  P+TL++++
Sbjct: 14  KMEDNKRKVLDEEAKASLDIWKYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLAELS 73

Query: 61  ASIASSSASPNISFLARIMALLVRRNIFAAHHPSDGGETLYGLTHSSRWLL--RDSDLTL 120
           +++   SASP  S L RIM  LV + IF      DG  T Y  T  SR L+  R    +L
Sbjct: 74  SAV---SASP--SHLRRIMRFLVHQGIFKEIPTKDGLATGYVNTPLSRRLMITRRDGKSL 133

Query: 121 APMILAELHPWLLDPWHRFAQGVREGGN-----LFHKANGLHFWDFAARNPQFNRLFNDA 180
           AP +L E  P +L PW R +  V    N      F   +G   W FA  NP  + + N+A
Sbjct: 134 APFVLFETTPEMLAPWLRLSSVVSSPVNGSTPPPFDAVHGKDVWSFAQDNPFLSDMINEA 193

Query: 181 MASTSKVVINAILSVYKDGFNSVGSLADVGGGIGGAVSEIVKAYPHMKGINYDLPHVVST 240
           MA  ++ V+  +       F+ V ++ DVGGG G  +  +VK +P +KG N+DLPHV+  
Sbjct: 194 MACDARRVVPRVAGACHGLFDGVTTMVDVGGGTGETMGMLVKEFPWIKGFNFDLPHVIEV 253

Query: 241 APAYPGVTHVGGDMFESVPKADAIFMKWILHDWDDKECVRILENCKKAIPEKTGKVIIVE 300
           A    GV +V GDMF+S+P  DAIF+KW+LHDW DK+C++IL+NCK+A+P   GKV+IVE
Sbjct: 254 AEVLDGVENVEGDMFDSIPACDAIFIKWVLHDWGDKDCIKILKNCKEAVPPNIGKVLIVE 313

Query: 301 VVLN--------PEGEGPFDDTGLYFDLLMLVH-SNGKERTEKEWKKILEEAGFLRYKLL 355
            V+          E +   +   L  D++M+ H S GKERT KEW  +L+EAGF RY++ 
Sbjct: 314 SVIGENKKTMIVDERDEKLEHVRLMLDMVMMAHTSTGKERTLKEWDFVLKEAGFARYEVR 373

[Close]

BLAST of MC02g0479 vs. TAIR 10
Match: AT5G54160.1 (O-methyltransferase 1 )

HSP 1 Score: 245.4 bits (625), Expect = 7.1e-65
Identity = 129/328 (39.33%), Postives = 206/328 (62.80%), Query Frame = 0


Query: 31  MALKSAVQLRLADIIKSHGSPLTLSQIAASIASSSASPNISFLARIMALLVRRNIFAAHH 90
           MALKSA++L L +I+  +GSP++ ++IA+ + + +    +  L RI+ LL   ++    +
Sbjct: 34  MALKSALELDLLEIMAKNGSPMSPTEIASKLPTKNPEAPV-MLDRILRLLTSYSVLTCSN 93

Query: 91  ---PSDGGETLYGLTHSSRWLLRDSD-LTLAPMILAELHPWLLDPWHRFAQGVREGGNLF 150
                DG E +YGL    ++L ++ D +++A + L      L++ W+     + +GG  F
Sbjct: 94  RKLSGDGVERIYGLGPVCKYLTKNEDGVSIAALCLMNQDKVLMESWYHLKDAILDGGIPF 153

Query: 151 HKANGLHFWDFAARNPQFNRLFNDAMASTSKVVINAILSVYKDGFNSVGSLADVGGGIGG 210
           +KA G+  +++   +P+FN++FN+ M++ S + +  IL  YK GF  + SL DVGGGIG 
Sbjct: 154 NKAYGMSAFEYHGTDPRFNKVFNNGMSNHSTITMKKILETYK-GFEGLTSLVDVGGGIGA 213

Query: 211 AVSEIVKAYPHMKGINYDLPHVVSTAPAYPGVTHVGGDMFESVPKADAIFMKWILHDWDD 270
            +  IV  YP++KGIN+DLPHV+  AP++PG+ HVGGDMF SVPK DAIFMKWI HDW D
Sbjct: 214 TLKMIVSKYPNLKGINFDLPHVIEDAPSHPGIEHVGGDMFVSVPKGDAIFMKWICHDWSD 273

Query: 271 KECVRILENCKKAIPEKTGKVIIVEVVLNPEGEGPFDDTG-LYFDLLMLVHS-NGKERTE 330
           + CV+ L+NC +++PE  GKVI+ E +L    +        ++ D +ML H+  GKERTE
Sbjct: 274 EHCVKFLKNCYESLPE-DGKVILAECILPETPDSSLSTKQVVHVDCIMLAHNPGGKERTE 333

Query: 331 KEWKKILEEAGFLRYKLLALPAITSIIE 353
           KE++ + + +GF   K++      ++IE
Sbjct: 334 KEFEALAKASGFKGIKVVCDAFGVNLIE 358

[Close]

BLAST of MC02g0479 vs. TAIR 10
Match: AT4G35150.1 (O-methyltransferase family protein )

HSP 1 Score: 223.8 bits (569), Expect = 2.2e-58
Identity = 134/364 (36.81%), Postives = 198/364 (54.40%), Query Frame = 0


Query: 2   MEPKEANEAEAMLRGQADIWRYMLCFADSMALKSAVQLRLADIIKSHGS--PLTLSQIAA 61
           ME  + N  +   +   DIWRY+  FAD  A K A+ L++ + I++H S  P+TLS++++
Sbjct: 1   MEESKRNLLDEEAKASLDIWRYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLSELSS 60

Query: 62  SIASSSASPNISFLARIMALLVRRNIFAAHHPSDGGETLYGLTHSSRWLLRDSDLTLAPM 121
           ++   SASP  S L RIM  LV + +F      DG  T Y  T  SR            M
Sbjct: 61  AV---SASP--SHLRRIMRFLVHQGLFKEVPTKDGLATGYTNTPLSR-----------RM 120

Query: 122 ILAELHPWLLDPWHRFAQGVREGGNLFHKANGLHFWDFAARNPQFNRLFNDAMASTSKVV 181
           ++ +LH                         G   W FA  N   ++L N+AMA  ++ V
Sbjct: 121 MITKLH-------------------------GKDLWAFAQDNLCHSQLINEAMACDARRV 180

Query: 182 INAILSVYKDGFNSVGSLADVGGGIGGAVSEIVKAYPHMKGINYDLPHVVSTAPAYPGVT 241
           +  +    +  F+ V ++ DVGGG G  +  +VK +P +KG N+DLPHV+  A    GV 
Sbjct: 181 VPRVAGACQGLFDGVATVVDVGGGTGETMGILVKEFPWIKGFNFDLPHVIEVAQVLDGVE 240

Query: 242 HVGGDMFESVPKADAIFMKWILHDWDDKECVRILENCKKAIPEKTGKVIIVEVVLN---- 301
           +V GDMF+S+P +DA+ +KW+LHDW DK+C++IL+NCK+A+    GKV+IVE V+     
Sbjct: 241 NVEGDMFDSIPASDAVIIKWVLHDWGDKDCIKILKNCKEAVLPNIGKVLIVECVIGEKKN 300

Query: 302 ----PEGEGPFDDTGLYFDLLMLVH-SNGKERTEKEWKKILEEAGFLRYKLLALPAITSI 355
                E +   +   L  D++M+VH S GKERT KEW  +L EAGF RY++     + S+
Sbjct: 301 TMIAEERDDKLEHVRLQLDMVMMVHTSTGKERTLKEWDFVLTEAGFARYEVRDFDDVQSL 323

[Close]

BLAST of MC02g0479 vs. TAIR 10
Match: AT1G77520.1 (O-methyltransferase family protein )

HSP 1 Score: 220.3 bits (560), Expect = 2.5e-57
Identity = 130/337 (38.58%), Postives = 192/337 (56.97%), Query Frame = 0


Query: 31  MALKSAVQLRLADIIKSHGSPLTLS--QIAASIASSSASPNIS-FLARIMALLVRRNIFA 90
           M LK+A +L + D I + G+   LS  +IA S+ +   +P     L R+++LLV  +I  
Sbjct: 44  MVLKAAFELGVIDTIAAAGNDTWLSPCEIACSLPTKPTNPEAPVLLDRMLSLLVSHSILK 103

Query: 91  AHHPSDGG-------ETLYGLTHSSRWLLRDSDLT--LAPMILAELHPWLLDPWHRFAQG 150
                 G        E +Y      ++ LRDSD T  L P+ +          W      
Sbjct: 104 CRMIETGENGRTGKIERVYAAEPVCKYFLRDSDGTGSLVPLFMLLHTQVFFKTWTNLKDV 163

Query: 151 VREGGNLFHKANGLHFWDFAARNPQFNRLFNDAMASTSKVVINAILSVYKDGFNSVGSLA 210
           + EG + F+ A+G+  +++   +  F  LFN AM+  S +++  +L VY+ GF  V +L 
Sbjct: 164 ILEGRDAFNSAHGMKIFEYINSDQPFAELFNRAMSEPSTMIMKKVLDVYR-GFEDVNTLV 223

Query: 211 DVGGGIGGAVSEIVKAYPHMKGINYDLPHVVSTAPAYPGVTHVGGDMFESVPKADAIFMK 270
           DVGGG G  +  +   YPH+KG+N+DL  V++ AP YPGV HV GDMF  VPK DA+FMK
Sbjct: 224 DVGGGNGTVLGLVTSKYPHIKGVNFDLAQVLTQAPFYPGVEHVSGDMFVEVPKGDAVFMK 283

Query: 271 WILHDWDDKECVRILENCKKAIPEKTGKVIIVEVVL--NPEGEGPFDDTGLYFDLLMLVH 330
           WILHDW D++C++IL+NC K++PEK GK+IIVE V    P+G     +T    DLLML  
Sbjct: 284 WILHDWGDEDCIKILKNCWKSLPEK-GKIIIVEFVTPKEPKGGDLSSNTVFAMDLLMLTQ 343

Query: 331 -SNGKERTEKEWKKILEEAGFLRYKLLALPAITSIIE 353
            S GKER+  +++ +   +GFLR +++ L    S+IE
Sbjct: 344 CSGGKERSLSQFENLAFASGFLRCEIICLAYSYSVIE 378

[Close]

BLAST of MC02g0479 vs. TAIR 10
Match: AT1G77530.1 (O-methyltransferase family protein )

HSP 1 Score: 218.0 bits (554), Expect = 1.2e-56
Identity = 131/337 (38.87%), Postives = 203/337 (60.24%), Query Frame = 0


Query: 31  MALKSAVQLRLADII--KSHGSPLTLSQIAASIASSSASPNIS-FLARIMALLVRRNIF- 90
           M LK+A++L + D I   S+G+ L+ S+IA S+ +   +P     L R++ LLV  +I  
Sbjct: 44  MVLKAALELGVIDTIAAASNGTWLSPSEIAVSLPNKPTNPEAPVLLDRMLRLLVSHSILK 103

Query: 91  -AAHHPSDGGET-----LYGLTHSSRWLLRDSD--LTLAPMILAELHPWLLDPWHRFAQG 150
                  + G+T     +Y      ++ L+DSD   +L+ ++L      +L  W      
Sbjct: 104 CCMVESRENGQTGKIERVYAAEPICKYFLKDSDGSGSLSSLLLLLHSQVILKTWTNLKDV 163

Query: 151 VREGGNLFHKANGLHFWDFAARNPQFNRLFNDAMASTSKVVINAILSVYKDGFNSVGSLA 210
           + EG + F  A+ +  +++ + + QF++LF+ AM+ +S +V+  +L  Y+ GF  V +L 
Sbjct: 164 ILEGKDAFSSAHDMRLFEYISSDDQFSKLFHRAMSESSTMVMKKVLEEYR-GFEDVNTLV 223

Query: 211 DVGGGIGGAVSEIVKAYPHMKGINYDLPHVVSTAPAYPGVTHVGGDMFESVPKADAIFMK 270
           DVGGGIG  +  I   YPH+KG+N+DL  V++ AP YPGV HV GDMF  VPK DAIFMK
Sbjct: 224 DVGGGIGTILGLITSKYPHIKGVNFDLAQVLTQAPFYPGVKHVSGDMFIEVPKGDAIFMK 283

Query: 271 WILHDWDDKECVRILENCKKAIPEKTGKVIIVEVV--LNPEGEGPFDDTGLYFDLLMLVH 330
           WILHDW D++C++IL+NC K++PEK GKVIIVE++  + P+      +T L  DLLML  
Sbjct: 284 WILHDWGDEDCIKILKNCWKSLPEK-GKVIIVEMITPMEPKPNDFSCNTVLGMDLLMLTQ 343

Query: 331 -SNGKERTEKEWKKILEEAGFLRYKLLALPAITSIIE 353
            S GKER+  +++ +   +GFL  +++ L    S+IE
Sbjct: 344 CSGGKERSLSQFENLAFASGFLLCEIICLSYSYSVIE 378

The following BLAST results are available for this feature:

BLAST of MC02g0479 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
B0ZB56	8.4e-111	54.72	Xanthohumol 4-O-methyltransferase OS=Humulus lupulus OX=3486 GN=OMT2 PE=1 SV=1	[more]
B0ZB55	6.9e-105	53.89	Desmethylxanthohumol 6'-O-methyltransferase OS=Humulus lupulus OX=3486 GN=OMT1 P...	[more]
Q6WUC2	8.4e-103	52.14	(R,S)-reticuline 7-O-methyltransferase OS=Papaver somniferum OX=3469 GN=7OMT PE=...	[more]
Q9LEL6	1.0e-79	43.82	(RS)-norcoclaurine 6-O-methyltransferase OS=Coptis japonica OX=3442 PE=1 SV=1	[more]
Q5C9L7	2.2e-79	42.20	(RS)-norcoclaurine 6-O-methyltransferase OS=Thalictrum flavum subsp. glaucum OX=...	[more]

BLAST of MC02g0479 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022140297.1	2.13e-260	100.00	xanthohumol 4'-O-methyltransferase-like [Momordica charantia]	[more]
XP_022140296.1	2.36e-232	89.83	xanthohumol 4'-O-methyltransferase-like [Momordica charantia]	[more]
XP_008448276.2	1.28e-202	77.25	PREDICTED: (R,S)-reticuline 7-O-methyltransferase-like [Cucumis melo] >KAA006210...	[more]
XP_038902222.1	7.41e-202	77.25	xanthohumol 4-O-methyltransferase-like [Benincasa hispida]	[more]
XP_022970718.1	8.63e-201	75.00	xanthohumol 4'-O-methyltransferase-like [Cucurbita maxima]	[more]

BLAST of MC02g0479 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CGG0	1.03e-260	100.00	xanthohumol 4'-O-methyltransferase-like OS=Momordica charantia OX=3673 GN=LOC111...	[more]
A0A6J1CFN9	1.14e-232	89.83	xanthohumol 4'-O-methyltransferase-like OS=Momordica charantia OX=3673 GN=LOC111...	[more]
A0A5A7V8T5	6.22e-203	77.25	(R,S)-reticuline 7-O-methyltransferase-like OS=Cucumis melo var. makuwa OX=11946...	[more]
A0A1S3BJA7	6.22e-203	77.25	(R,S)-reticuline 7-O-methyltransferase-like OS=Cucumis melo OX=3656 GN=LOC103490...	[more]
A0A6J1I4R1	4.18e-201	75.00	xanthohumol 4'-O-methyltransferase-like OS=Cucurbita maxima OX=3661 GN=LOC111469...	[more]

BLAST of MC02g0479 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G35160.1	1.2e-67	39.25	O-methyltransferase family protein	[more]
AT5G54160.1	7.1e-65	39.33	O-methyltransferase 1	[more]
AT4G35150.1	2.2e-58	36.81	O-methyltransferase family protein	[more]
AT1G77520.1	2.5e-57	38.58	O-methyltransferase family protein	[more]
AT1G77530.1	1.2e-56	38.87	O-methyltransferase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001077	O-methyltransferase domain	PFAM	PF00891	Methyltransf_2	coord: 132..336 e-value: 3.2E-67 score: 225.9
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 110..356 e-value: 1.2E-84 score: 285.4
None	No IPR available	PIRSR	PIRSR005739-1	PIRSR005739-1	coord: 30..354 e-value: 1.7E-93 score: 310.7
None	No IPR available	PANTHER	PTHR11746:SF235	(R,S)-RETICULINE 7-O-METHYLTRANSFERASE-LIKE ISOFORM X1	coord: 6..355
IPR012967	Plant methyltransferase dimerisation	PFAM	PF08100	Dimerisation	coord: 31..81 e-value: 1.3E-11 score: 44.3
IPR036388	Winged helix-like DNA-binding domain superfamily	GENE3D	1.10.10.10		coord: 3..108 e-value: 1.6E-32 score: 113.3
IPR016461	O-methyltransferase COMT-type	PIRSF	PIRSF005739	O-mtase	coord: 1..356 e-value: 3.0E-126 score: 419.1
IPR016461	O-methyltransferase COMT-type	PANTHER	PTHR11746	O-METHYLTRANSFERASE	coord: 6..355
IPR016461	O-methyltransferase COMT-type	PROSITE	PS51683	SAM_OMT_II	coord: 21..355 score: 81.218597
IPR036390	Winged helix DNA-binding domain superfamily	SUPERFAMILY	46785	"Winged helix" DNA-binding domain	coord: 11..112
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 114..354

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC02g0479.1	MC02g0479.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0019438	aromatic compound biosynthetic process
biological_process	GO:0032259	methylation
molecular_function	GO:0008171	O-methyltransferase activity
molecular_function	GO:0046983	protein dimerization activity
molecular_function	GO:0008757	S-adenosylmethionine-dependent methyltransferase activity
molecular_function	GO:0008168	methyltransferase activity