MC02g0477 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC02g0477
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Epidermal patterning factor-like protein
Location	MC02: 3968325 .. 3968507 (+)
RNA-Seq Expression	MC02g0477
Synteny	MC02g0477

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AGGAGAGGCGAGACAGTGAAAATTGCAGGGTCGAGGTTACCAGATTGCTCACACGCTTGTGGGTGGTGCTCGCCATGCAGATTAGTGATGGTTAGGTACATTTGTGGTGCTGCAGTGGAAGATTCAGAGACGTGTCCAACGGCTTACAAGTGCATGTGCACCTTTAAATCCTTCCCTGTTCCA

mRNA sequence

AGGAGAGGCGAGACAGTGAAAATTGCAGGGTCGAGGTTACCAGATTGCTCACACGCTTGTGGGTGGTGCTCGCCATGCAGATTAGTGATGGTTAGGTACATTTGTGGTGCTGCAGTGGAAGATTCAGAGACGTGTCCAACGGCTTACAAGTGCATGTGCACCTTTAAATCCTTCCCTGTTCCA

Coding sequence (CDS)

AGGAGAGGCGAGACAGTGAAAATTGCAGGGTCGAGGTTACCAGATTGCTCACACGCTTGTGGGTGGTGCTCGCCATGCAGATTAGTGATGGTTAGGTACATTTGTGGTGCTGCAGTGGAAGATTCAGAGACGTGTCCAACGGCTTACAAGTGCATGTGCACCTTTAAATCCTTCCCTGTTCCA

Protein sequence

RRGETVKIAGSRLPDCSHACGWCSPCRLVMVRYICGAAVEDSETCPTAYKCMCTFKSFPVP

Homology

[Close]

BLAST of MC02g0477 vs. ExPASy Swiss-Prot
Match: Q8S8I4 (Protein EPIDERMAL PATTERNING FACTOR 1 OS=Arabidopsis thaliana OX=3702 GN=EPF1 PE=1 SV=1)

HSP 1 Score: 104.0 bits (258), Expect = 6.2e-22
Identity = 45/61 (73.77%), Postives = 54/61 (88.52%), Query Frame = 0


Query: 1   RRGETVKIAGSRLPDCSHACGWCSPCRLVMVRYICGAAVEDSETCPTAYKCMCTFKSFPV 60
           RR +TV++AGSRLPDCSHACG CSPCRLVMV ++C A+VE++ETCP AYKCMC  KS+PV
Sbjct: 45  RRPDTVQVAGSRLPDCSHACGSCSPCRLVMVSFVC-ASVEEAETCPMAYKCMCNNKSYPV 104

Query: 61  P 62
           P
Sbjct: 105 P 104

[Close]

BLAST of MC02g0477 vs. ExPASy Swiss-Prot
Match: C4B8C5 (EPIDERMAL PATTERNING FACTOR-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=EPFL7 PE=1 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 2.4e-10
Identity = 28/55 (50.91%), Postives = 36/55 (65.45%), Query Frame = 0


Query: 7   KIAGSRLPDCSHACGWCSPCRLVMVRYICGAAVEDSETCPTAYKCMCTFKSFPVP 62
           K +GS +PDCS+ACG C PC+LV++   C A    SE CP  YKC+C  K + VP
Sbjct: 119 KASGSSIPDCSNACGPCKPCKLVVISSTCSA----SEACPLVYKCLCKGKYYHVP 169

[Close]

BLAST of MC02g0477 vs. ExPASy Swiss-Prot
Match: Q8LC53 (Protein EPIDERMAL PATTERNING FACTOR 2 OS=Arabidopsis thaliana OX=3702 GN=EPF2 PE=1 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 3.0e-08
Identity = 25/52 (48.08%), Postives = 33/52 (63.46%), Query Frame = 0


Query: 10  GSRLPDCSHACGWCSPCRLVMVRYICGAAVEDSETCPTAYKCMCTFKSFPVP 62
           GS LPDCS+ACG CSPC+ VM+ + C  A    E+C   Y+C C  + + VP
Sbjct: 70  GSSLPDCSYACGACSPCKRVMISFECSVA----ESCSVIYRCTCRGRYYHVP 117

[Close]

BLAST of MC02g0477 vs. NCBI nr
Match: XP_022140689.1 (protein EPIDERMAL PATTERNING FACTOR 1-like [Momordica charantia])

HSP 1 Score: 138 bits (348), Expect = 7.17e-41
Identity = 61/61 (100.00%), Postives = 61/61 (100.00%), Query Frame = 0


Query: 1   RRGETVKIAGSRLPDCSHACGWCSPCRLVMVRYICGAAVEDSETCPTAYKCMCTFKSFPV 60
           RRGETVKIAGSRLPDCSHACGWCSPCRLVMVRYICGAAVEDSETCPTAYKCMCTFKSFPV
Sbjct: 40  RRGETVKIAGSRLPDCSHACGWCSPCRLVMVRYICGAAVEDSETCPTAYKCMCTFKSFPV 99

[Close]

BLAST of MC02g0477 vs. NCBI nr
Match: KAG7034925.1 (Protein EPIDERMAL PATTERNING FACTOR 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 110 bits (276), Expect = 6.45e-30
Identity = 49/60 (81.67%), Postives = 55/60 (91.67%), Query Frame = 0


Query: 2  RGETVKIAGSRLPDCSHACGWCSPCRLVMVRYICGAAVEDSETCPTAYKCMCTFKSFPVP 61
          RGETV++AGSRLPDCSHACG C+PCRLVMVRY C ++VEDSETCP AYKCMC+ KSFPVP
Sbjct: 40 RGETVEVAGSRLPDCSHACGSCAPCRLVMVRYFC-SSVEDSETCPLAYKCMCSHKSFPVP 98

[Close]

BLAST of MC02g0477 vs. NCBI nr
Match: NP_179684.1 (epidermal patterning factor 1 [Arabidopsis thaliana] >Q8S8I4.1 RecName: Full=Protein EPIDERMAL PATTERNING FACTOR 1; Contains: RecName: Full=MEPF1; Flags: Precursor [Arabidopsis thaliana] >KAG7636879.1 hypothetical protein ISN45_At02g014610 [Arabidopsis thaliana x Arabidopsis arenosa] >KAG7641496.1 hypothetical protein ISN44_As02g015110 [Arabidopsis suecica] >AAM15214.1 predicted protein [Arabidopsis thaliana] >AEC07090.1 epidermal patterning factor 1 [Arabidopsis thaliana] >OAP11009.1 EPF1 [Arabidopsis thaliana])

HSP 1 Score: 103 bits (256), Expect = 8.56e-27
Identity = 45/61 (73.77%), Postives = 54/61 (88.52%), Query Frame = 0


Query: 1   RRGETVKIAGSRLPDCSHACGWCSPCRLVMVRYICGAAVEDSETCPTAYKCMCTFKSFPV 60
           RR +TV++AGSRLPDCSHACG CSPCRLVMV ++C A+VE++ETCP AYKCMC  KS+PV
Sbjct: 45  RRPDTVQVAGSRLPDCSHACGSCSPCRLVMVSFVC-ASVEEAETCPMAYKCMCNNKSYPV 104

[Close]

BLAST of MC02g0477 vs. NCBI nr
Match: KAG5224840.1 (Membrane lipoprotein [Salix suchowensis])

HSP 1 Score: 103 bits (257), Expect = 9.24e-27
Identity = 46/61 (75.41%), Postives = 54/61 (88.52%), Query Frame = 0


Query: 1   RRGETVKIAGSRLPDCSHACGWCSPCRLVMVRYICGAAVEDSETCPTAYKCMCTFKSFPV 60
           RRG+TV+IAGSRLPDCS ACG CSPCRLVMV +IC A++E++ETCP AYKCMC  KS+PV
Sbjct: 60  RRGDTVQIAGSRLPDCSRACGSCSPCRLVMVSFIC-ASIEEAETCPMAYKCMCNNKSYPV 119

[Close]

BLAST of MC02g0477 vs. NCBI nr
Match: KFK40466.1 (hypothetical protein AALP_AA3G376600 [Arabis alpina])

HSP 1 Score: 102 bits (255), Expect = 1.29e-26
Identity = 44/61 (72.13%), Postives = 54/61 (88.52%), Query Frame = 0


Query: 1   RRGETVKIAGSRLPDCSHACGWCSPCRLVMVRYICGAAVEDSETCPTAYKCMCTFKSFPV 60
           RR +TV++AGSRLPDCSHACG CSPCRLVMV ++C A++E++ETCP AYKCMC  KS+PV
Sbjct: 47  RRPDTVQVAGSRLPDCSHACGSCSPCRLVMVSFVC-ASIEEAETCPMAYKCMCNNKSYPV 106

[Close]

BLAST of MC02g0477 vs. ExPASy TrEMBL
Match: A0A6J1CGD5 (Epidermal patterning factor-like protein OS=Momordica charantia OX=3673 GN=LOC111011291 PE=3 SV=1)

HSP 1 Score: 138 bits (348), Expect = 3.47e-41
Identity = 61/61 (100.00%), Postives = 61/61 (100.00%), Query Frame = 0


Query: 1   RRGETVKIAGSRLPDCSHACGWCSPCRLVMVRYICGAAVEDSETCPTAYKCMCTFKSFPV 60
           RRGETVKIAGSRLPDCSHACGWCSPCRLVMVRYICGAAVEDSETCPTAYKCMCTFKSFPV
Sbjct: 40  RRGETVKIAGSRLPDCSHACGWCSPCRLVMVRYICGAAVEDSETCPTAYKCMCTFKSFPV 99

[Close]

BLAST of MC02g0477 vs. ExPASy TrEMBL
Match: A0A178VX97 (Epidermal patterning factor-like protein OS=Arabidopsis thaliana OX=3702 GN=AXX17_At2g16320 PE=3 SV=1)

HSP 1 Score: 103 bits (256), Expect = 4.14e-27
Identity = 45/61 (73.77%), Postives = 54/61 (88.52%), Query Frame = 0


Query: 1   RRGETVKIAGSRLPDCSHACGWCSPCRLVMVRYICGAAVEDSETCPTAYKCMCTFKSFPV 60
           RR +TV++AGSRLPDCSHACG CSPCRLVMV ++C A+VE++ETCP AYKCMC  KS+PV
Sbjct: 45  RRPDTVQVAGSRLPDCSHACGSCSPCRLVMVSFVC-ASVEEAETCPMAYKCMCNNKSYPV 104

[Close]

BLAST of MC02g0477 vs. ExPASy TrEMBL
Match: A0A087HEB4 (Epidermal patterning factor-like protein OS=Arabis alpina OX=50452 GN=AALP_AA3G376600 PE=3 SV=1)

HSP 1 Score: 102 bits (255), Expect = 6.23e-27
Identity = 44/61 (72.13%), Postives = 54/61 (88.52%), Query Frame = 0


Query: 1   RRGETVKIAGSRLPDCSHACGWCSPCRLVMVRYICGAAVEDSETCPTAYKCMCTFKSFPV 60
           RR +TV++AGSRLPDCSHACG CSPCRLVMV ++C A++E++ETCP AYKCMC  KS+PV
Sbjct: 47  RRPDTVQVAGSRLPDCSHACGSCSPCRLVMVSFVC-ASIEEAETCPMAYKCMCNNKSYPV 106

[Close]

BLAST of MC02g0477 vs. ExPASy TrEMBL
Match: A0A6D2HH05 (Epidermal patterning factor-like protein OS=Microthlaspi erraticum OX=1685480 GN=MERR_LOCUS1261 PE=3 SV=1)

HSP 1 Score: 102 bits (255), Expect = 6.78e-27
Identity = 44/61 (72.13%), Postives = 54/61 (88.52%), Query Frame = 0


Query: 1   RRGETVKIAGSRLPDCSHACGWCSPCRLVMVRYICGAAVEDSETCPTAYKCMCTFKSFPV 60
           RR +TV++AGSRLPDCSHACG CSPCRLVMV ++C A++E++ETCP AYKCMC  KS+PV
Sbjct: 50  RRPDTVQVAGSRLPDCSHACGSCSPCRLVMVSFVC-ASIEEAETCPMAYKCMCNNKSYPV 109

[Close]

BLAST of MC02g0477 vs. ExPASy TrEMBL
Match: A0A397Y3J3 (Epidermal patterning factor-like protein OS=Brassica campestris OX=3711 GN=BRAA09T41253Z PE=3 SV=1)

HSP 1 Score: 102 bits (254), Expect = 7.89e-27
Identity = 44/61 (72.13%), Postives = 54/61 (88.52%), Query Frame = 0


Query: 1   RRGETVKIAGSRLPDCSHACGWCSPCRLVMVRYICGAAVEDSETCPTAYKCMCTFKSFPV 60
           RR +TV++AGSRLPDCSHACG CSPCRLVMV ++C A++E++ETCP AYKCMC  KS+PV
Sbjct: 43  RRPDTVQVAGSRLPDCSHACGSCSPCRLVMVSFVC-ASIEEAETCPMAYKCMCKNKSYPV 102

[Close]

BLAST of MC02g0477 vs. TAIR 10
Match: AT2G20875.1 (epidermal patterning factor 1 )

HSP 1 Score: 104.0 bits (258), Expect = 4.4e-23
Identity = 45/61 (73.77%), Postives = 54/61 (88.52%), Query Frame = 0


Query: 1   RRGETVKIAGSRLPDCSHACGWCSPCRLVMVRYICGAAVEDSETCPTAYKCMCTFKSFPV 60
           RR +TV++AGSRLPDCSHACG CSPCRLVMV ++C A+VE++ETCP AYKCMC  KS+PV
Sbjct: 45  RRPDTVQVAGSRLPDCSHACGSCSPCRLVMVSFVC-ASVEEAETCPMAYKCMCNNKSYPV 104

Query: 61  P 62
           P
Sbjct: 105 P 104

[Close]

BLAST of MC02g0477 vs. TAIR 10
Match: AT1G71866.1 (LOCATED IN: endomembrane system; BEST Arabidopsis thaliana protein match is: Putative membrane lipoprotein (TAIR:AT1G34245.1). )

HSP 1 Score: 65.5 bits (158), Expect = 1.7e-11
Identity = 28/55 (50.91%), Postives = 36/55 (65.45%), Query Frame = 0


Query: 7   KIAGSRLPDCSHACGWCSPCRLVMVRYICGAAVEDSETCPTAYKCMCTFKSFPVP 62
           K +GS +PDCS+ACG C PC+LV++   C A    SE CP  YKC+C  K + VP
Sbjct: 119 KASGSSIPDCSNACGPCKPCKLVVISSTCSA----SEACPLVYKCLCKGKYYHVP 169

[Close]

BLAST of MC02g0477 vs. TAIR 10
Match: AT1G34245.1 (Putative membrane lipoprotein )

HSP 1 Score: 58.5 bits (140), Expect = 2.1e-09
Identity = 25/52 (48.08%), Postives = 33/52 (63.46%), Query Frame = 0


Query: 10  GSRLPDCSHACGWCSPCRLVMVRYICGAAVEDSETCPTAYKCMCTFKSFPVP 62
           GS LPDCS+ACG CSPC+ VM+ + C  A    E+C   Y+C C  + + VP
Sbjct: 70  GSSLPDCSYACGACSPCKRVMISFECSVA----ESCSVIYRCTCRGRYYHVP 117

The following BLAST results are available for this feature:

BLAST of MC02g0477 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8S8I4	6.2e-22	73.77	Protein EPIDERMAL PATTERNING FACTOR 1 OS=Arabidopsis thaliana OX=3702 GN=EPF1 PE...	[more]
C4B8C5	2.4e-10	50.91	EPIDERMAL PATTERNING FACTOR-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=EP...	[more]
Q8LC53	3.0e-08	48.08	Protein EPIDERMAL PATTERNING FACTOR 2 OS=Arabidopsis thaliana OX=3702 GN=EPF2 PE...	[more]

BLAST of MC02g0477 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022140689.1	7.17e-41	100.00	protein EPIDERMAL PATTERNING FACTOR 1-like [Momordica charantia]	[more]
KAG7034925.1	6.45e-30	81.67	Protein EPIDERMAL PATTERNING FACTOR 1, partial [Cucurbita argyrosperma subsp. ar...	[more]
NP_179684.1	8.56e-27	73.77	epidermal patterning factor 1 [Arabidopsis thaliana] >Q8S8I4.1 RecName: Full=Pro...	[more]
KAG5224840.1	9.24e-27	75.41	Membrane lipoprotein [Salix suchowensis]	[more]
KFK40466.1	1.29e-26	72.13	hypothetical protein AALP_AA3G376600 [Arabis alpina]	[more]

BLAST of MC02g0477 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CGD5	3.47e-41	100.00	Epidermal patterning factor-like protein OS=Momordica charantia OX=3673 GN=LOC11...	[more]
A0A178VX97	4.14e-27	73.77	Epidermal patterning factor-like protein OS=Arabidopsis thaliana OX=3702 GN=AXX1...	[more]
A0A087HEB4	6.23e-27	72.13	Epidermal patterning factor-like protein OS=Arabis alpina OX=50452 GN=AALP_AA3G3...	[more]
A0A6D2HH05	6.78e-27	72.13	Epidermal patterning factor-like protein OS=Microthlaspi erraticum OX=1685480 GN...	[more]
A0A397Y3J3	7.89e-27	72.13	Epidermal patterning factor-like protein OS=Brassica campestris OX=3711 GN=BRAA0...	[more]

BLAST of MC02g0477 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G20875.1	4.4e-23	73.77	epidermal patterning factor 1	[more]
AT1G71866.1	1.7e-11	50.91	LOCATED IN: endomembrane system; BEST Arabidopsis thaliana protein match is: Put...	[more]
AT1G34245.1	2.1e-09	48.08	Putative membrane lipoprotein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PFAM	PF17181	EPF	coord: 9..59 e-value: 2.3E-13 score: 49.9
None	No IPR available	PANTHER	PTHR33109:SF41	PROTEIN EPIDERMAL PATTERNING FACTOR 1	coord: 1..61
IPR039455	EPIDERMAL PATTERNING FACTOR-like protein	PANTHER	PTHR33109	EPIDERMAL PATTERNING FACTOR-LIKE PROTEIN 4	coord: 1..61

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC02g0477.1	MC02g0477.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010052	guard cell differentiation
biological_process	GO:2000122	negative regulation of stomatal complex development
biological_process	GO:0010375	stomatal complex patterning
biological_process	GO:0010374	stomatal complex development
cellular_component	GO:0005576	extracellular region