MC02g0389 (gene) Bitter gourd (Dali-11) v1

BLAST of MC02g0389 vs. ExPASy Swiss-Prot
Match: Q93XW5 (Nitrile-specifier protein 5 OS=Arabidopsis thaliana OX=3702 GN=NSP5 PE=2 SV=1)

HSP 1 Score: 378.3 bits (970), Expect = 9.1e-104
Identity = 169/320 (52.81%), Postives = 222/320 (69.38%), Query Frame = 0

Query: 1   MAPTHGKWIKIHQNGTSPGARSSHAITILGQNAYAFGGEFTPRLPVDNKLHVFDLDELTW 60
           M P   KW+K+ Q G  PGARSSHA+T++G   Y FGGE  P + +DN L+VFDL+   W
Sbjct: 1   MCPVENKWLKVGQKGAGPGARSSHAMTVVGNKVYCFGGELKPTIHIDNDLYVFDLETQEW 60

Query: 61  TVADVSGDVPPPRVGVTMAAVGRIVYVFGGRDSDHKELNELYSFDTSINKWTLLSGGDSG 120
           ++A  +G+ P P  GV+M  +G  +YV+GGRD D +  N L+S+DT  N+W LL+  + G
Sbjct: 61  SIAPATGEAPFPCFGVSMVTIGSTIYVYGGRD-DKRRYNGLHSYDTETNEWKLLAPVEEG 120

Query: 121 PPHRSYHSSAADDRRLYIFGGCGNAGRLNDLWAYDTVDGKWIEHPPAGEKLKGRGGPGLV 180
            P RSYHS A DDR++Y+FGG    GR+N L AYD VD KW+E+P AGE  KGRG PGLV
Sbjct: 121 LPGRSYHSMAGDDRKVYVFGGVTAKGRVNTLHAYDVVDQKWVEYPAAGEACKGRGAPGLV 180

Query: 181 VAQGKIWVVYGFAGMEIDDVHYFDPAQNVWAQVDTTGEKPTARSVFSTFAIGKYVFIYGG 240
           V +G+IWV++GF G E+ D+H FD A   W  V+TTG+ P ARSVF   + GKY+ IYGG
Sbjct: 181 VVEGRIWVLFGFDGNELGDIHCFDLASEQWKAVETTGDVPAARSVFPAVSYGKYIVIYGG 240

Query: 241 EVDPSDLGHLGAGKFAGEVYVLDTVSLVWSRVEDGLGLENHPGPRGWCAYSVGRKGGELG 300
           E +P +L H+GAGK +GEVY LDT +LVW R+  G   E  P  RGWCA++   K GE G
Sbjct: 241 EEEPHELMHMGAGKMSGEVYQLDTETLVWERIVCG-NEEEKPSQRGWCAFTKAVKDGEEG 300

Query: 301 LVVYGGNSPSNDRLGDIYFF 321
           L+V+GGNSP+N+RL D+ F+
Sbjct: 301 LLVHGGNSPTNERLDDLVFW 318

BLAST of MC02g0389 vs. ExPASy Swiss-Prot
Match: O04316 (Nitrile-specifier protein 4 OS=Arabidopsis thaliana OX=3702 GN=NSP4 PE=2 SV=1)

HSP 1 Score: 342.0 bits (876), Expect = 7.2e-93
Identity = 152/324 (46.91%), Postives = 214/324 (66.05%), Query Frame = 0

Query: 1   MAPT---HGKWIKIHQNGTSPGARSSHAITILGQNAYAFGGEFTPRLPVDNKLHVFDLDE 60
           ++PT   HGKW K+ +NG  PG R SH I  +G   Y+FGGEFTP  P+D  L+VFD++ 
Sbjct: 292 LSPTPNLHGKWTKVDENGDGPGLRCSHDIAQVGNKIYSFGGEFTPNQPIDKHLYVFDIES 351

Query: 61  LTWTVADVSGDVPP-PRVGVTMAAVGRIVYVFGGRDSDHKELNELYSFDTSINKWTLLSG 120
            TW+++  +GD+P    +GV M ++G  +YVFGGRD+  ++ N  YSFDT+ N+W LL+ 
Sbjct: 352 RTWSISPATGDIPTLSCLGVCMVSIGSTLYVFGGRDAS-RQYNGFYSFDTTTNEWKLLTP 411

Query: 121 GDSGPPHRSYHSSAADDRRLYIFGGCGNAGRLNDLWAYDTVDGKWIEHPPAGEKLKGRGG 180
            + GP  RS+HS AAD+  +Y+FGG     RLN L +Y+ VD KW      G+ L  RGG
Sbjct: 412 VEEGPTPRSFHSMAADEENVYVFGGVSATARLNTLDSYNIVDKKWFHCSTPGDSLTARGG 471

Query: 181 PGLVVAQGKIWVVYGFAGMEIDDVHYFDPAQNVWAQVDTTGEKPTARSVFSTFAIGKYVF 240
            GL V QGK+WVVYGF G E+DDVHY+DP Q+ W QV+T G +P+ RSVF++ A+GK++ 
Sbjct: 472 AGLEVVQGKVWVVYGFNGCEVDDVHYYDPVQDKWTQVETFGVRPSERSVFASAALGKHIV 531

Query: 241 IYGGEVDPSDLGHLGAGKFAGEVYVLDTVSLVWSRVEDGLGLENHPGPRGWCAYSVGRKG 300
           I+GGE+    L H+G G+     + LDT +L W R++   G E  P  RGW A +    G
Sbjct: 532 IFGGEIAMDPLAHVGPGQLTDGTFALDTETLQWERLDKFGGEEETPSSRGWTASTTATIG 591

Query: 301 GELGLVVYGGNSPSNDRLGDIYFF 321
           G+ GLV++GG +P+NDR  D++F+
Sbjct: 592 GKKGLVMHGGKAPTNDRFDDLFFY 614

BLAST of MC02g0389 vs. ExPASy Swiss-Prot
Match: Q9SDM9 (Nitrile-specifier protein 1 OS=Arabidopsis thaliana OX=3702 GN=NSP1 PE=1 SV=2)

HSP 1 Score: 339.3 bits (869), Expect = 4.7e-92
Identity = 153/316 (48.42%), Postives = 208/316 (65.82%), Query Frame = 0

Query: 6   GKWIKIHQNGTSPGARSSHAITILGQNAYAFGGEFTPRLPVDNKLHVFDLDELTWTVADV 65
           GKWIK+ Q G  PG R SH I  +G   Y+FGGEFTP  P+D  L+VFDL+  TW+++  
Sbjct: 151 GKWIKVEQKGEGPGLRCSHGIAQVGNKIYSFGGEFTPNQPIDKHLYVFDLETRTWSISPA 210

Query: 66  SGDVPP-PRVGVTMAAVGRIVYVFGGRDSDHKELNELYSFDTSINKWTLLSGGDSGPPHR 125
           +GDVP    +GV M +VG  +YVFGGRD+  ++ N  YSFDT+ N+W LL+  + GP  R
Sbjct: 211 TGDVPHLSCLGVRMVSVGSTLYVFGGRDAS-RQYNGFYSFDTTTNEWKLLTPVEEGPTPR 270

Query: 126 SYHSSAADDRRLYIFGGCGNAGRLNDLWAYDTVDGKWIEHPPAGEKLKGRGGPGLVVAQG 185
           S+HS AAD+  +Y+FGG     RLN L +Y+ VD KW      G+ L  RGG GL V QG
Sbjct: 271 SFHSMAADEENVYVFGGVSATARLNTLDSYNIVDKKWFHCSTPGDSLTARGGAGLEVVQG 330

Query: 186 KIWVVYGFAGMEIDDVHYFDPAQNVWAQVDTTGEKPTARSVFSTFAIGKYVFIYGGEVDP 245
           K+WVVYGF G E+DDVHY+DP Q+ W QV+T G +P+ RSVF++ AIGK++ I+GGE+  
Sbjct: 331 KVWVVYGFNGCEVDDVHYYDPVQDKWTQVETFGVRPSERSVFASAAIGKHIVIFGGEIAM 390

Query: 246 SDLGHLGAGKFAGEVYVLDTVSLVWSRVEDGLGLENHPGPRGWCAYSVGRKGGELGLVVY 305
             L H+G G+     + LDT +L W R++   G E  P  RGW A +     G+ GLV++
Sbjct: 391 DPLAHVGPGQLTDGTFALDTETLQWERLDKFGGEEETPSSRGWTASTTATIDGKKGLVMH 450

Query: 306 GGNSPSNDRLGDIYFF 321
           GG +P+NDR  D++F+
Sbjct: 451 GGKAPTNDRFDDLFFY 465

BLAST of MC02g0389 vs. ExPASy Swiss-Prot
Match: O49326 (Nitrile-specifier protein 2 OS=Arabidopsis thaliana OX=3702 GN=NSP2 PE=2 SV=1)

HSP 1 Score: 324.7 bits (831), Expect = 1.2e-87
Identity = 151/317 (47.63%), Postives = 206/317 (64.98%), Query Frame = 0

Query: 6   GKWIKIHQNGTSPGARSSHAITILGQNAYAFGGEFTPRLPVDNKLHVFDLDELTWTVADV 65
           GKWIK+ Q G  PG R SH I  +G   ++FGGE TP  P+D  L+VFDL+  TW+++  
Sbjct: 152 GKWIKVEQKGEGPGPRCSHDIAQVGNKIFSFGGELTPNQPIDKHLYVFDLETRTWSISPA 211

Query: 66  SGDVPP-PRVGVTMAAVGRIVYVFGGRDSDHKELNELYSFDTSINKWTLLSGGDSGPPHR 125
           +GDVP    +GV M ++G  +YVFGGRD+  K  N  YSFDT+ N+W LL+  + GP  R
Sbjct: 212 TGDVPNLSCLGVRMVSIGSSLYVFGGRDASRK-YNGFYSFDTTKNEWKLLTPVEQGPTPR 271

Query: 126 SYHSSAADDRRLYIFGGCGNAGRLNDLWAYDTVDGKWIEHPPAGEKLKGRGGPGLVVAQG 185
           S+HS  AD+  +Y+FGG     RL  L AY+ VD KW++    G     RGG GL V QG
Sbjct: 272 SFHSMTADENNVYVFGGVSATVRLKTLDAYNIVDHKWVQCSTPGGSCSVRGGAGLEVVQG 331

Query: 186 KIWVVYGFAGMEIDDVHYFDPAQNVWAQVDTTGEKPTARSVFSTFAIGKYVFIYGGEVDP 245
           K+WVVYGF G E+DDVH +DPAQ+ W QV+T GEKP ARSVF++  +GK++ ++GGE+  
Sbjct: 332 KVWVVYGFNGCEVDDVHCYDPAQDKWTQVETFGEKPCARSVFASAVVGKHILVFGGEIAM 391

Query: 246 SDLGHLGAGKFAGEVYVLDTVSLVWSRVEDGLGLENH-PGPRGWCAYSVGRKGGELGLVV 305
               H G G+ +G  + LDT +L W ++ D LG E   P  RGW A + G   G+ GLV+
Sbjct: 392 DPKAHEGPGQLSGGTFALDTETLKWEKL-DKLGEEEETPSIRGWSASTTGTIDGKKGLVM 451

Query: 306 YGGNSPSNDRLGDIYFF 321
           +GG + +NDR GD++F+
Sbjct: 452 FGGKAQTNDRFGDLFFY 466

BLAST of MC02g0389 vs. ExPASy Swiss-Prot
Match: O04318 (Nitrile-specifier protein 3 OS=Arabidopsis thaliana OX=3702 GN=NSP3 PE=2 SV=1)

HSP 1 Score: 322.0 bits (824), Expect = 7.7e-87
Identity = 146/316 (46.20%), Postives = 201/316 (63.61%), Query Frame = 0

Query: 6   GKWIKIHQNGTSPGARSSHAITILGQNAYAFGGEFTPRLPVDNKLHVFDLDELTWTVADV 65
           G WIK+ QNG  PG R SH I  +G   Y+FGGE  P  P+D  L+VFDL+  TW++A  
Sbjct: 151 GNWIKVEQNGEGPGLRCSHGIAQVGNKIYSFGGELIPNQPIDKHLYVFDLETRTWSIAPA 210

Query: 66  SGDVPP-PRVGVTMAAVGRIVYVFGGRDSDHKELNELYSFDTSINKWTLLSGGDSGPPHR 125
           +GDVP    +GV M +VG  +Y FGGRD   ++ N  YSFDT+ N+W LL+  + GP  R
Sbjct: 211 TGDVPHLSCLGVRMVSVGSTLYTFGGRDFS-RQYNGFYSFDTTTNEWKLLTPVEEGPTPR 270

Query: 126 SYHSSAADDRRLYIFGGCGNAGRLNDLWAYDTVDGKWIEHPPAGEKLKGRGGPGLVVAQG 185
           S+HS AAD+  +Y+FGG G   R+  L +Y+ VD  W      G+    RGG GL V QG
Sbjct: 271 SFHSMAADEENVYVFGGVGAMDRIKTLDSYNIVDKTWFHCSNPGDSFSIRGGAGLEVVQG 330

Query: 186 KIWVVYGFAGMEIDDVHYFDPAQNVWAQVDTTGEKPTARSVFSTFAIGKYVFIYGGEVDP 245
           K+W+VYGF G E+DDVH++DPA++ W QV+T G KP  RSVF++ AIGK++ I+GGE+  
Sbjct: 331 KVWIVYGFNGCEVDDVHFYDPAEDKWTQVETFGVKPNERSVFASAAIGKHIVIFGGEIAM 390

Query: 246 SDLGHLGAGKFAGEVYVLDTVSLVWSRVEDGLGLENHPGPRGWCAYSVGRKGGELGLVVY 305
               H+G G+     + LDT +L W R++     E  P  RGW A + G   G+ GLV++
Sbjct: 391 DPRAHVGPGQLIDGTFALDTETLQWERLDK---FEGTPSSRGWTASTTGTIDGKKGLVMH 450

Query: 306 GGNSPSNDRLGDIYFF 321
           GG +P+NDR  D++F+
Sbjct: 451 GGKAPTNDRFDDLFFY 462

BLAST of MC02g0389 vs. NCBI nr
Match: XP_022971053.1 (nitrile-specifier protein 5 [Cucurbita maxima])

HSP 1 Score: 608 bits (1567), Expect = 1.30e-218
Identity = 281/322 (87.27%), Postives = 301/322 (93.48%), Query Frame = 0

Query: 1   MAPTHGKWIKIHQNGTSPGARSSHAITILGQNAYAFGGEFTPRLPVDNKLHVFDLDELTW 60
           MAP +GKW KI QNG  PGARSSHAITI+G+ AYAFGGEF+PR+PVDNKLHVFDLDE TW
Sbjct: 1   MAPMNGKWTKIDQNGAGPGARSSHAITIVGKKAYAFGGEFSPRIPVDNKLHVFDLDEHTW 60

Query: 61  TVADVSGDVPPPRVGVTMAAVGRIVYVFGGRDSDHKELNELYSFDTSINKWTLLSGGDSG 120
           TVA VSGDVPPPRVGVTM AVG+IVYVFGGRD +HKELNELYSFDTSINKWTLLSGG+SG
Sbjct: 61  TVASVSGDVPPPRVGVTMDAVGQIVYVFGGRDLEHKELNELYSFDTSINKWTLLSGGESG 120

Query: 121 PPHRSYHSSAADDRRLYIFGGCGNAGRLNDLWAYDTVDGKWIEHPPAGEKLKGRGGPGLV 180
           PPHRSYHS+ ADDRR+YIFGGCGN+GRLNDLWAYDT+DGKWIE+P AGE LKGRGGPGL 
Sbjct: 121 PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIDGKWIEYPSAGEGLKGRGGPGLA 180

Query: 181 VAQGKIWVVYGFAGMEIDDVHYFDPAQNVWAQVDTTGEKPTARSVFSTFAIGKYVFIYGG 240
           VAQ KIWVVYGFAGME+DDVHYFDPAQN WAQV+TTGE+PT RSVFST AIGKYV+IYGG
Sbjct: 181 VAQRKIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTPRSVFSTCAIGKYVYIYGG 240

Query: 241 EVDPSDLGHLGAGKFAGEVYVLDTVSLVWSRVEDGLGLENHPGPRGWCAYSVGRKGGELG 300
           EVDPSDLGHLGAGKFAGEVYVLDT +L WSRVEDGLGLENHPGPRGWCA+SVGRKGGELG
Sbjct: 241 EVDPSDLGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGWCAFSVGRKGGELG 300

Query: 301 LVVYGGNSPSNDRLGDIYFFIP 322
           L+VYGGNSPSNDRLGDIYFF P
Sbjct: 301 LLVYGGNSPSNDRLGDIYFFTP 322

BLAST of MC02g0389 vs. NCBI nr
Match: XP_022947523.1 (nitrile-specifier protein 5 [Cucurbita moschata] >KAG6604919.1 Nitrile-specifier protein 5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 607 bits (1565), Expect = 2.63e-218
Identity = 279/322 (86.65%), Postives = 303/322 (94.10%), Query Frame = 0

Query: 1   MAPTHGKWIKIHQNGTSPGARSSHAITILGQNAYAFGGEFTPRLPVDNKLHVFDLDELTW 60
           MAP +GKW KI QNG  PGARSSHAIT++G+ AYAFGGEF+PR+PVDN+LHVFDLDE TW
Sbjct: 1   MAPMNGKWTKIDQNGAGPGARSSHAITVVGKKAYAFGGEFSPRIPVDNELHVFDLDEHTW 60

Query: 61  TVADVSGDVPPPRVGVTMAAVGRIVYVFGGRDSDHKELNELYSFDTSINKWTLLSGGDSG 120
           TVA VSGDVPPPRVGVTMAAVG+IVYVFGGRD +HKELNELYSFDTSINKWTLLSGG+SG
Sbjct: 61  TVASVSGDVPPPRVGVTMAAVGQIVYVFGGRDLEHKELNELYSFDTSINKWTLLSGGESG 120

Query: 121 PPHRSYHSSAADDRRLYIFGGCGNAGRLNDLWAYDTVDGKWIEHPPAGEKLKGRGGPGLV 180
           PPHRSYHS+ ADDRR+YIFGGCGN+GRLNDLWAYDT++GKWIE+P AGE LKGRGGPGL 
Sbjct: 121 PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIEGKWIEYPSAGEGLKGRGGPGLA 180

Query: 181 VAQGKIWVVYGFAGMEIDDVHYFDPAQNVWAQVDTTGEKPTARSVFSTFAIGKYVFIYGG 240
           VAQGKIWVVYGFAGME+DDVHYFDPAQN WAQV+TTGE+PTARSVFST AIGKYV+IYGG
Sbjct: 181 VAQGKIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTARSVFSTCAIGKYVYIYGG 240

Query: 241 EVDPSDLGHLGAGKFAGEVYVLDTVSLVWSRVEDGLGLENHPGPRGWCAYSVGRKGGELG 300
           EVDPSD+GHLGAGKFAGEVYVLDT +L WSRVEDGLGLENHPGPRGW A+SVGRKGGELG
Sbjct: 241 EVDPSDMGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGWSAFSVGRKGGELG 300

Query: 301 LVVYGGNSPSNDRLGDIYFFIP 322
           L+VYGGNSPSNDRLGDIYFF P
Sbjct: 301 LLVYGGNSPSNDRLGDIYFFTP 322

BLAST of MC02g0389 vs. NCBI nr
Match: XP_023533621.1 (nitrile-specifier protein 5 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 607 bits (1564), Expect = 3.73e-218
Identity = 280/322 (86.96%), Postives = 302/322 (93.79%), Query Frame = 0

Query: 1   MAPTHGKWIKIHQNGTSPGARSSHAITILGQNAYAFGGEFTPRLPVDNKLHVFDLDELTW 60
           MAP +GKW KI QNG  PGARSSHAITI+G+ AYAFGGEF+PR+PVDN+LHVFDLDE TW
Sbjct: 1   MAPMNGKWTKIDQNGAGPGARSSHAITIVGKKAYAFGGEFSPRIPVDNELHVFDLDEHTW 60

Query: 61  TVADVSGDVPPPRVGVTMAAVGRIVYVFGGRDSDHKELNELYSFDTSINKWTLLSGGDSG 120
           TVA VSGDVPPPRVGVTMAAVG+IVYVFGGRD +HKELNELYS DTSINKWTLLSGG+SG
Sbjct: 61  TVASVSGDVPPPRVGVTMAAVGQIVYVFGGRDLEHKELNELYSLDTSINKWTLLSGGESG 120

Query: 121 PPHRSYHSSAADDRRLYIFGGCGNAGRLNDLWAYDTVDGKWIEHPPAGEKLKGRGGPGLV 180
           PPHRSYHS+ ADDRR+YIFGGCGN+GRLNDLWAYDT++GKWIE+P AGE LKGRGGPGL 
Sbjct: 121 PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIEGKWIEYPSAGEGLKGRGGPGLA 180

Query: 181 VAQGKIWVVYGFAGMEIDDVHYFDPAQNVWAQVDTTGEKPTARSVFSTFAIGKYVFIYGG 240
           VAQGKIWVVYGFAGME+DDVHYFDPAQN WAQV+TTGE+PTARSVFST AIGKYV+IYGG
Sbjct: 181 VAQGKIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTARSVFSTCAIGKYVYIYGG 240

Query: 241 EVDPSDLGHLGAGKFAGEVYVLDTVSLVWSRVEDGLGLENHPGPRGWCAYSVGRKGGELG 300
           EVDPSDLGHLGAGKFAGEVYVLDT +L WSRVEDGLGLENHPGPRGWCA+SVGRKG ELG
Sbjct: 241 EVDPSDLGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGWCAFSVGRKGRELG 300

Query: 301 LVVYGGNSPSNDRLGDIYFFIP 322
           L+VYGGNSPSNDRLGDIYFF P
Sbjct: 301 LLVYGGNSPSNDRLGDIYFFTP 322

BLAST of MC02g0389 vs. NCBI nr
Match: KAG7026998.1 (Nitrile-specifier protein 5 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 605 bits (1559), Expect = 2.16e-217
Identity = 278/322 (86.34%), Postives = 302/322 (93.79%), Query Frame = 0

Query: 1   MAPTHGKWIKIHQNGTSPGARSSHAITILGQNAYAFGGEFTPRLPVDNKLHVFDLDELTW 60
           MAP +GKW KI QNG  PGARSSHAIT++G+ AYAFGGEF+PR+PVDN+LHVFDLDE TW
Sbjct: 1   MAPMNGKWTKIDQNGAGPGARSSHAITVVGKKAYAFGGEFSPRIPVDNELHVFDLDEHTW 60

Query: 61  TVADVSGDVPPPRVGVTMAAVGRIVYVFGGRDSDHKELNELYSFDTSINKWTLLSGGDSG 120
           TVA VSGDVPPPRVGVTMAAVG+IVYVFGGRD +HKELNELYSFDTSI KWTLLSGG+SG
Sbjct: 61  TVASVSGDVPPPRVGVTMAAVGQIVYVFGGRDLEHKELNELYSFDTSIKKWTLLSGGESG 120

Query: 121 PPHRSYHSSAADDRRLYIFGGCGNAGRLNDLWAYDTVDGKWIEHPPAGEKLKGRGGPGLV 180
           PPHRSYHS+ ADDRR+YIFGGCGN+GRLNDLWAYDT++GKWIE+P AGE LKGRGGPGL 
Sbjct: 121 PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIEGKWIEYPSAGEGLKGRGGPGLA 180

Query: 181 VAQGKIWVVYGFAGMEIDDVHYFDPAQNVWAQVDTTGEKPTARSVFSTFAIGKYVFIYGG 240
           VAQGKIWVVYGFAGME+DDVHYFDPAQN WAQV+TTGE+PTARSVFST AIGKYV+IYGG
Sbjct: 181 VAQGKIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTARSVFSTCAIGKYVYIYGG 240

Query: 241 EVDPSDLGHLGAGKFAGEVYVLDTVSLVWSRVEDGLGLENHPGPRGWCAYSVGRKGGELG 300
           EVDPSD+GHLGAGKFAGEVYVLDT +L WSRVEDGLGLENHPGPRGW A+SVGRKGGELG
Sbjct: 241 EVDPSDMGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGWSAFSVGRKGGELG 300

Query: 301 LVVYGGNSPSNDRLGDIYFFIP 322
           L+VYGGNSPSNDRLGDIYFF P
Sbjct: 301 LLVYGGNSPSNDRLGDIYFFTP 322

BLAST of MC02g0389 vs. NCBI nr
Match: XP_038900567.1 (nitrile-specifier protein 5 [Benincasa hispida])

HSP 1 Score: 588 bits (1515), Expect = 1.14e-210
Identity = 272/323 (84.21%), Postives = 294/323 (91.02%), Query Frame = 0

Query: 1   MAPTHGKWIKIHQNGTSPGARSSHAITILGQNAYAFGGEFTPRLPVDNKLHVFDLDELTW 60
           MAPT+GKW KI Q    PGARSSHAITI+ Q AYAFGGEF+PR+PVDNKLHVFDL E  W
Sbjct: 1   MAPTNGKWTKIDQKTAGPGARSSHAITIVAQKAYAFGGEFSPRVPVDNKLHVFDLHEHIW 60

Query: 61  TVADVSGDVPPPRVGVTMAAVGRIVYVFGGRDSDHKELNELYSFDTSINKWTLLSGGDSG 120
           TVA VSGDVPPPRVGVTMA+VGRI+YVFGGRDS+H ELNELYSFDTS +KWTLLSG DSG
Sbjct: 61  TVAVVSGDVPPPRVGVTMASVGRIIYVFGGRDSEHTELNELYSFDTSNSKWTLLSGSDSG 120

Query: 121 PPHRSYHSSAADDRRLYIFGGCGNAGRLNDLWAYDTVDGKWIEHPPAGEKLKGRGGPGLV 180
           PPHRSYHS+ ADDRR+YIFGGCGN+GRLNDLWAYDTVDGKW+E+P  GE+LKGRGGPGL 
Sbjct: 121 PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTVDGKWMEYPAGGERLKGRGGPGLA 180

Query: 181 VAQGKIWVVYGFAGMEIDDVHYFDPAQNVWAQVDTTGEKPTARSVFSTFAIGKYVFIYGG 240
           VAQ KIWVVYGFAGME+DDVHYFD AQN WAQV+T+GEKPTARSVF T AIGKYV+IYGG
Sbjct: 181 VAQDKIWVVYGFAGMEMDDVHYFDVAQNQWAQVETSGEKPTARSVFCTCAIGKYVYIYGG 240

Query: 241 EVDPSDLGHLGAGKFAGEVYVLDTVSLVWSRVEDGLGLENHPGPRGWCAYSVGRKGGELG 300
           EVDPSDLGHLGAGKFAGEVYVLDT +L WSRVEDGLG+ENHPGPRGWCA+SVGR G ELG
Sbjct: 241 EVDPSDLGHLGAGKFAGEVYVLDTEALEWSRVEDGLGMENHPGPRGWCAFSVGRNGEELG 300

Query: 301 LVVYGGNSPSNDRLGDIYFFIPC 323
           L+VYGGNSPSNDRLGDIYFF PC
Sbjct: 301 LLVYGGNSPSNDRLGDIYFFAPC 323

BLAST of MC02g0389 vs. ExPASy TrEMBL
Match: A0A6J1I5Q1 (nitrile-specifier protein 5 OS=Cucurbita maxima OX=3661 GN=LOC111469839 PE=4 SV=1)

HSP 1 Score: 608 bits (1567), Expect = 6.31e-219
Identity = 281/322 (87.27%), Postives = 301/322 (93.48%), Query Frame = 0

Query: 1   MAPTHGKWIKIHQNGTSPGARSSHAITILGQNAYAFGGEFTPRLPVDNKLHVFDLDELTW 60
           MAP +GKW KI QNG  PGARSSHAITI+G+ AYAFGGEF+PR+PVDNKLHVFDLDE TW
Sbjct: 1   MAPMNGKWTKIDQNGAGPGARSSHAITIVGKKAYAFGGEFSPRIPVDNKLHVFDLDEHTW 60

Query: 61  TVADVSGDVPPPRVGVTMAAVGRIVYVFGGRDSDHKELNELYSFDTSINKWTLLSGGDSG 120
           TVA VSGDVPPPRVGVTM AVG+IVYVFGGRD +HKELNELYSFDTSINKWTLLSGG+SG
Sbjct: 61  TVASVSGDVPPPRVGVTMDAVGQIVYVFGGRDLEHKELNELYSFDTSINKWTLLSGGESG 120

Query: 121 PPHRSYHSSAADDRRLYIFGGCGNAGRLNDLWAYDTVDGKWIEHPPAGEKLKGRGGPGLV 180
           PPHRSYHS+ ADDRR+YIFGGCGN+GRLNDLWAYDT+DGKWIE+P AGE LKGRGGPGL 
Sbjct: 121 PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIDGKWIEYPSAGEGLKGRGGPGLA 180

Query: 181 VAQGKIWVVYGFAGMEIDDVHYFDPAQNVWAQVDTTGEKPTARSVFSTFAIGKYVFIYGG 240
           VAQ KIWVVYGFAGME+DDVHYFDPAQN WAQV+TTGE+PT RSVFST AIGKYV+IYGG
Sbjct: 181 VAQRKIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTPRSVFSTCAIGKYVYIYGG 240

Query: 241 EVDPSDLGHLGAGKFAGEVYVLDTVSLVWSRVEDGLGLENHPGPRGWCAYSVGRKGGELG 300
           EVDPSDLGHLGAGKFAGEVYVLDT +L WSRVEDGLGLENHPGPRGWCA+SVGRKGGELG
Sbjct: 241 EVDPSDLGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGWCAFSVGRKGGELG 300

Query: 301 LVVYGGNSPSNDRLGDIYFFIP 322
           L+VYGGNSPSNDRLGDIYFF P
Sbjct: 301 LLVYGGNSPSNDRLGDIYFFTP 322

BLAST of MC02g0389 vs. ExPASy TrEMBL
Match: A0A6J1G6U4 (nitrile-specifier protein 5 OS=Cucurbita moschata OX=3662 GN=LOC111451365 PE=4 SV=1)

HSP 1 Score: 607 bits (1565), Expect = 1.27e-218
Identity = 279/322 (86.65%), Postives = 303/322 (94.10%), Query Frame = 0

Query: 1   MAPTHGKWIKIHQNGTSPGARSSHAITILGQNAYAFGGEFTPRLPVDNKLHVFDLDELTW 60
           MAP +GKW KI QNG  PGARSSHAIT++G+ AYAFGGEF+PR+PVDN+LHVFDLDE TW
Sbjct: 1   MAPMNGKWTKIDQNGAGPGARSSHAITVVGKKAYAFGGEFSPRIPVDNELHVFDLDEHTW 60

Query: 61  TVADVSGDVPPPRVGVTMAAVGRIVYVFGGRDSDHKELNELYSFDTSINKWTLLSGGDSG 120
           TVA VSGDVPPPRVGVTMAAVG+IVYVFGGRD +HKELNELYSFDTSINKWTLLSGG+SG
Sbjct: 61  TVASVSGDVPPPRVGVTMAAVGQIVYVFGGRDLEHKELNELYSFDTSINKWTLLSGGESG 120

Query: 121 PPHRSYHSSAADDRRLYIFGGCGNAGRLNDLWAYDTVDGKWIEHPPAGEKLKGRGGPGLV 180
           PPHRSYHS+ ADDRR+YIFGGCGN+GRLNDLWAYDT++GKWIE+P AGE LKGRGGPGL 
Sbjct: 121 PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIEGKWIEYPSAGEGLKGRGGPGLA 180

Query: 181 VAQGKIWVVYGFAGMEIDDVHYFDPAQNVWAQVDTTGEKPTARSVFSTFAIGKYVFIYGG 240
           VAQGKIWVVYGFAGME+DDVHYFDPAQN WAQV+TTGE+PTARSVFST AIGKYV+IYGG
Sbjct: 181 VAQGKIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTARSVFSTCAIGKYVYIYGG 240

Query: 241 EVDPSDLGHLGAGKFAGEVYVLDTVSLVWSRVEDGLGLENHPGPRGWCAYSVGRKGGELG 300
           EVDPSD+GHLGAGKFAGEVYVLDT +L WSRVEDGLGLENHPGPRGW A+SVGRKGGELG
Sbjct: 241 EVDPSDMGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGWSAFSVGRKGGELG 300

Query: 301 LVVYGGNSPSNDRLGDIYFFIP 322
           L+VYGGNSPSNDRLGDIYFF P
Sbjct: 301 LLVYGGNSPSNDRLGDIYFFTP 322

BLAST of MC02g0389 vs. ExPASy TrEMBL
Match: A0A1S3CLA8 (nitrile-specifier protein 5 OS=Cucumis melo OX=3656 GN=LOC103502104 PE=4 SV=1)

HSP 1 Score: 585 bits (1507), Expect = 9.11e-210
Identity = 266/323 (82.35%), Postives = 295/323 (91.33%), Query Frame = 0

Query: 1   MAPTHGKWIKIHQNGTSPGARSSHAITILGQNAYAFGGEFTPRLPVDNKLHVFDLDELTW 60
           MAPT+GKWIKI Q    PGARSSHAI I+ Q AY+FGGEF+PRLPVDNKLHVFDL +  W
Sbjct: 1   MAPTNGKWIKIDQKTIGPGARSSHAIAIVDQKAYSFGGEFSPRLPVDNKLHVFDLHDHIW 60

Query: 61  TVADVSGDVPPPRVGVTMAAVGRIVYVFGGRDSDHKELNELYSFDTSINKWTLLSGGDSG 120
           TV  VSGD+PPPRVGVTMA++G+I+YVFGGRD++HKELNELYSFDTSINKWTLLSGG+SG
Sbjct: 61  TVVAVSGDIPPPRVGVTMASIGQIIYVFGGRDTEHKELNELYSFDTSINKWTLLSGGESG 120

Query: 121 PPHRSYHSSAADDRRLYIFGGCGNAGRLNDLWAYDTVDGKWIEHPPAGEKLKGRGGPGLV 180
           PPHRSYHS+ ADD  +YIFGGCGN+GRLNDLWAYDTVDGKWIE+P AGE+LKGRGGPGL 
Sbjct: 121 PPHRSYHSTTADDHNVYIFGGCGNSGRLNDLWAYDTVDGKWIEYPAAGEELKGRGGPGLA 180

Query: 181 VAQGKIWVVYGFAGMEIDDVHYFDPAQNVWAQVDTTGEKPTARSVFSTFAIGKYVFIYGG 240
           VAQ KIWVVYGFAGME+DDVHYFDP QN WAQV+T G++PTARSVF T AIGKYV+IYGG
Sbjct: 181 VAQDKIWVVYGFAGMEMDDVHYFDPDQNKWAQVETRGQRPTARSVFCTCAIGKYVYIYGG 240

Query: 241 EVDPSDLGHLGAGKFAGEVYVLDTVSLVWSRVEDGLGLENHPGPRGWCAYSVGRKGGELG 300
           EVDPSD+GHLGAGKFAGEVYVLDT  L WSRV+DGLG+E+HPGPRGWCA+SVGRKGGELG
Sbjct: 241 EVDPSDVGHLGAGKFAGEVYVLDTEVLEWSRVDDGLGVEDHPGPRGWCAFSVGRKGGELG 300

Query: 301 LVVYGGNSPSNDRLGDIYFFIPC 323
           L+VYGGNSPSNDRLGDIYFF PC
Sbjct: 301 LLVYGGNSPSNDRLGDIYFFAPC 323

BLAST of MC02g0389 vs. ExPASy TrEMBL
Match: A0A5D3CBW1 (Nitrile-specifier protein 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold796G00270 PE=4 SV=1)

HSP 1 Score: 585 bits (1507), Expect = 9.11e-210
Identity = 266/323 (82.35%), Postives = 295/323 (91.33%), Query Frame = 0

Query: 1   MAPTHGKWIKIHQNGTSPGARSSHAITILGQNAYAFGGEFTPRLPVDNKLHVFDLDELTW 60
           MAPT+GKWIKI Q    PGARSSHAI I+ Q AY+FGGEF+PRLPVDNKLHVFDL +  W
Sbjct: 1   MAPTNGKWIKIDQKTIGPGARSSHAIAIVDQKAYSFGGEFSPRLPVDNKLHVFDLHDHIW 60

Query: 61  TVADVSGDVPPPRVGVTMAAVGRIVYVFGGRDSDHKELNELYSFDTSINKWTLLSGGDSG 120
           TV  VSGD+PPPRVGVTMA++G+I+YVFGGRD++HKELNELYSFDTSINKWTLLSGG+SG
Sbjct: 61  TVVAVSGDIPPPRVGVTMASIGQIIYVFGGRDTEHKELNELYSFDTSINKWTLLSGGESG 120

Query: 121 PPHRSYHSSAADDRRLYIFGGCGNAGRLNDLWAYDTVDGKWIEHPPAGEKLKGRGGPGLV 180
           PPHRSYHS+ ADD  +YIFGGCGN+GRLNDLWAYDTVDGKWIE+P AGE+LKGRGGPGL 
Sbjct: 121 PPHRSYHSTTADDHNVYIFGGCGNSGRLNDLWAYDTVDGKWIEYPAAGEELKGRGGPGLA 180

Query: 181 VAQGKIWVVYGFAGMEIDDVHYFDPAQNVWAQVDTTGEKPTARSVFSTFAIGKYVFIYGG 240
           VAQ KIWVVYGFAGME+DDVHYFDP QN WAQV+T G++PTARSVF T AIGKYV+IYGG
Sbjct: 181 VAQDKIWVVYGFAGMEMDDVHYFDPDQNKWAQVETRGQRPTARSVFCTCAIGKYVYIYGG 240

Query: 241 EVDPSDLGHLGAGKFAGEVYVLDTVSLVWSRVEDGLGLENHPGPRGWCAYSVGRKGGELG 300
           EVDPSD+GHLGAGKFAGEVYVLDT  L WSRV+DGLG+E+HPGPRGWCA+SVGRKGGELG
Sbjct: 241 EVDPSDVGHLGAGKFAGEVYVLDTEVLEWSRVDDGLGVEDHPGPRGWCAFSVGRKGGELG 300

Query: 301 LVVYGGNSPSNDRLGDIYFFIPC 323
           L+VYGGNSPSNDRLGDIYFF PC
Sbjct: 301 LLVYGGNSPSNDRLGDIYFFAPC 323

BLAST of MC02g0389 vs. ExPASy TrEMBL
Match: A0A0A0KDY9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G112500 PE=4 SV=1)

HSP 1 Score: 579 bits (1493), Expect = 1.24e-207
Identity = 264/323 (81.73%), Postives = 290/323 (89.78%), Query Frame = 0

Query: 1   MAPTHGKWIKIHQNGTSPGARSSHAITILGQNAYAFGGEFTPRLPVDNKLHVFDLDELTW 60
           MAPT G WIKI Q  T PGARSSHAI I+ Q AY+FGGEF+PRLPVDNKLH+FDL +  W
Sbjct: 1   MAPTKGNWIKIDQKTTGPGARSSHAIAIVDQKAYSFGGEFSPRLPVDNKLHIFDLHDHIW 60

Query: 61  TVADVSGDVPPPRVGVTMAAVGRIVYVFGGRDSDHKELNELYSFDTSINKWTLLSGGDSG 120
           TV   SGD+PPPRVGVTMA+VG+I+YVFGGRD++HKELNELYSFDTSINKWTLLSGGD G
Sbjct: 61  TVGAASGDIPPPRVGVTMASVGQIIYVFGGRDTEHKELNELYSFDTSINKWTLLSGGDYG 120

Query: 121 PPHRSYHSSAADDRRLYIFGGCGNAGRLNDLWAYDTVDGKWIEHPPAGEKLKGRGGPGLV 180
           PP RSYHS+ ADDRR+YIFGGCGN+GRLNDLWAYDT+DGKWIE+P AGE LKGRGGPGL 
Sbjct: 121 PPQRSYHSTTADDRRIYIFGGCGNSGRLNDLWAYDTIDGKWIEYPAAGEGLKGRGGPGLA 180

Query: 181 VAQGKIWVVYGFAGMEIDDVHYFDPAQNVWAQVDTTGEKPTARSVFSTFAIGKYVFIYGG 240
           VAQ KIWVVYGFAGME+DDVHYF P QN W QV+T GE+PTARSVF T AIGKYV+IYGG
Sbjct: 181 VAQDKIWVVYGFAGMEMDDVHYFSPEQNKWTQVETKGERPTARSVFCTCAIGKYVYIYGG 240

Query: 241 EVDPSDLGHLGAGKFAGEVYVLDTVSLVWSRVEDGLGLENHPGPRGWCAYSVGRKGGELG 300
           EVDPSDLGHLGAGKF GEVYVLDT +L WSRVEDGLGLE+HPGPRGWCA++VGRKGGELG
Sbjct: 241 EVDPSDLGHLGAGKFVGEVYVLDTEALEWSRVEDGLGLEDHPGPRGWCAFAVGRKGGELG 300

Query: 301 LVVYGGNSPSNDRLGDIYFFIPC 323
           L+VYGGNSPSNDRLGDI+FF PC
Sbjct: 301 LLVYGGNSPSNDRLGDIHFFAPC 323

BLAST of MC02g0389 vs. TAIR 10
Match: AT3G07720.1 (Galactose oxidase/kelch repeat superfamily protein )

HSP 1 Score: 471.1 bits (1211), Expect = 7.3e-133
Identity = 207/320 (64.69%), Postives = 250/320 (78.12%), Query Frame = 0

Query: 3   PTHGKWIKIHQNGTSPGARSSHAITILGQNAYAFGGEFTPRLPVDNKLHVFDLDELTWTV 62
           P  GKW+++ Q GT PGARSSHAI ++G   YAFGGEF PR+PVDN+L+VFDL+  TW++
Sbjct: 5   PMEGKWVQLKQKGTGPGARSSHAIALVGNKMYAFGGEFQPRVPVDNQLYVFDLETQTWSI 64

Query: 63  ADVSGDVPPPRVGVTMAAVGRIVYVFGGRDSDHKELNELYSFDTSINKWTLLSGGDSGPP 122
            + SGD PPPRVGV MAAVG I+Y FGGRDS H+ELNELY F+T  N+W LLS G++GP 
Sbjct: 65  QEASGDAPPPRVGVAMAAVGPIIYFFGGRDSTHQELNELYCFNTLTNQWKLLSSGETGPQ 124

Query: 123 HRSYHSSAADDRRLYIFGGCGNAGRLNDLWAYDTVDGKWIEHPPAGEKLKGRGGPGLVVA 182
           +RSYHS  AD + +Y+FGGCG  GRLNDLWAY+ VD KWI+ P  GE  +GRGGPGL V 
Sbjct: 125 NRSYHSITADSQNVYVFGGCGVDGRLNDLWAYNVVDQKWIKFPSPGEACRGRGGPGLEVV 184

Query: 183 QGKIWVVYGFAGMEIDDVHYFDPAQNVWAQVDTTGEKPTARSVFSTFAIGKYVFIYGGEV 242
           QGKIWVVYGFAG E DDVH FD A+  W +V+T GEKP+ARSVFST  +GK + I GGE+
Sbjct: 185 QGKIWVVYGFAGEEADDVHCFDIAKGEWKEVETKGEKPSARSVFSTAVVGKQILISGGEI 244

Query: 243 DPSDLGHLGAGKFAGEVYVLDTVSLVWSRVEDGLGLENHPGPRGWCAYSVGRKGGELGLV 302
           DPSDLGH+GAG F G+ Y LDT +L W + EDG+G   HPGPRGWCA++ G + G+ GL+
Sbjct: 245 DPSDLGHMGAGCFTGDAYGLDTETLEWRKWEDGVGSVEHPGPRGWCAFAAGSRDGKQGLL 304

Query: 303 VYGGNSPSNDRLGDIYFFIP 323
           VYGGNSPSNDRL DI+FF P
Sbjct: 305 VYGGNSPSNDRLDDIFFFTP 324

BLAST of MC02g0389 vs. TAIR 10
Match: AT5G48180.1 (nitrile specifier protein 5 )

HSP 1 Score: 378.3 bits (970), Expect = 6.4e-105
Identity = 169/320 (52.81%), Postives = 222/320 (69.38%), Query Frame = 0

Query: 1   MAPTHGKWIKIHQNGTSPGARSSHAITILGQNAYAFGGEFTPRLPVDNKLHVFDLDELTW 60
           M P   KW+K+ Q G  PGARSSHA+T++G   Y FGGE  P + +DN L+VFDL+   W
Sbjct: 1   MCPVENKWLKVGQKGAGPGARSSHAMTVVGNKVYCFGGELKPTIHIDNDLYVFDLETQEW 60

Query: 61  TVADVSGDVPPPRVGVTMAAVGRIVYVFGGRDSDHKELNELYSFDTSINKWTLLSGGDSG 120
           ++A  +G+ P P  GV+M  +G  +YV+GGRD D +  N L+S+DT  N+W LL+  + G
Sbjct: 61  SIAPATGEAPFPCFGVSMVTIGSTIYVYGGRD-DKRRYNGLHSYDTETNEWKLLAPVEEG 120

Query: 121 PPHRSYHSSAADDRRLYIFGGCGNAGRLNDLWAYDTVDGKWIEHPPAGEKLKGRGGPGLV 180
            P RSYHS A DDR++Y+FGG    GR+N L AYD VD KW+E+P AGE  KGRG PGLV
Sbjct: 121 LPGRSYHSMAGDDRKVYVFGGVTAKGRVNTLHAYDVVDQKWVEYPAAGEACKGRGAPGLV 180

Query: 181 VAQGKIWVVYGFAGMEIDDVHYFDPAQNVWAQVDTTGEKPTARSVFSTFAIGKYVFIYGG 240
           V +G+IWV++GF G E+ D+H FD A   W  V+TTG+ P ARSVF   + GKY+ IYGG
Sbjct: 181 VVEGRIWVLFGFDGNELGDIHCFDLASEQWKAVETTGDVPAARSVFPAVSYGKYIVIYGG 240

Query: 241 EVDPSDLGHLGAGKFAGEVYVLDTVSLVWSRVEDGLGLENHPGPRGWCAYSVGRKGGELG 300
           E +P +L H+GAGK +GEVY LDT +LVW R+  G   E  P  RGWCA++   K GE G
Sbjct: 241 EEEPHELMHMGAGKMSGEVYQLDTETLVWERIVCG-NEEEKPSQRGWCAFTKAVKDGEEG 300

Query: 301 LVVYGGNSPSNDRLGDIYFF 321
           L+V+GGNSP+N+RL D+ F+
Sbjct: 301 LLVHGGNSPTNERLDDLVFW 318

BLAST of MC02g0389 vs. TAIR 10
Match: AT3G16410.1 (nitrile specifier protein 4 )

HSP 1 Score: 342.0 bits (876), Expect = 5.1e-94
Identity = 152/324 (46.91%), Postives = 214/324 (66.05%), Query Frame = 0

Query: 1   MAPT---HGKWIKIHQNGTSPGARSSHAITILGQNAYAFGGEFTPRLPVDNKLHVFDLDE 60
           ++PT   HGKW K+ +NG  PG R SH I  +G   Y+FGGEFTP  P+D  L+VFD++ 
Sbjct: 292 LSPTPNLHGKWTKVDENGDGPGLRCSHDIAQVGNKIYSFGGEFTPNQPIDKHLYVFDIES 351

Query: 61  LTWTVADVSGDVPP-PRVGVTMAAVGRIVYVFGGRDSDHKELNELYSFDTSINKWTLLSG 120
            TW+++  +GD+P    +GV M ++G  +YVFGGRD+  ++ N  YSFDT+ N+W LL+ 
Sbjct: 352 RTWSISPATGDIPTLSCLGVCMVSIGSTLYVFGGRDAS-RQYNGFYSFDTTTNEWKLLTP 411

Query: 121 GDSGPPHRSYHSSAADDRRLYIFGGCGNAGRLNDLWAYDTVDGKWIEHPPAGEKLKGRGG 180
            + GP  RS+HS AAD+  +Y+FGG     RLN L +Y+ VD KW      G+ L  RGG
Sbjct: 412 VEEGPTPRSFHSMAADEENVYVFGGVSATARLNTLDSYNIVDKKWFHCSTPGDSLTARGG 471

Query: 181 PGLVVAQGKIWVVYGFAGMEIDDVHYFDPAQNVWAQVDTTGEKPTARSVFSTFAIGKYVF 240
            GL V QGK+WVVYGF G E+DDVHY+DP Q+ W QV+T G +P+ RSVF++ A+GK++ 
Sbjct: 472 AGLEVVQGKVWVVYGFNGCEVDDVHYYDPVQDKWTQVETFGVRPSERSVFASAALGKHIV 531

Query: 241 IYGGEVDPSDLGHLGAGKFAGEVYVLDTVSLVWSRVEDGLGLENHPGPRGWCAYSVGRKG 300
           I+GGE+    L H+G G+     + LDT +L W R++   G E  P  RGW A +    G
Sbjct: 532 IFGGEIAMDPLAHVGPGQLTDGTFALDTETLQWERLDKFGGEEETPSSRGWTASTTATIG 591

Query: 301 GELGLVVYGGNSPSNDRLGDIYFF 321
           G+ GLV++GG +P+NDR  D++F+
Sbjct: 592 GKKGLVMHGGKAPTNDRFDDLFFY 614

BLAST of MC02g0389 vs. TAIR 10
Match: AT3G16400.1 (nitrile specifier protein 1 )

HSP 1 Score: 339.3 bits (869), Expect = 3.3e-93
Identity = 153/316 (48.42%), Postives = 208/316 (65.82%), Query Frame = 0

Query: 6   GKWIKIHQNGTSPGARSSHAITILGQNAYAFGGEFTPRLPVDNKLHVFDLDELTWTVADV 65
           GKWIK+ Q G  PG R SH I  +G   Y+FGGEFTP  P+D  L+VFDL+  TW+++  
Sbjct: 151 GKWIKVEQKGEGPGLRCSHGIAQVGNKIYSFGGEFTPNQPIDKHLYVFDLETRTWSISPA 210

Query: 66  SGDVPP-PRVGVTMAAVGRIVYVFGGRDSDHKELNELYSFDTSINKWTLLSGGDSGPPHR 125
           +GDVP    +GV M +VG  +YVFGGRD+  ++ N  YSFDT+ N+W LL+  + GP  R
Sbjct: 211 TGDVPHLSCLGVRMVSVGSTLYVFGGRDAS-RQYNGFYSFDTTTNEWKLLTPVEEGPTPR 270

Query: 126 SYHSSAADDRRLYIFGGCGNAGRLNDLWAYDTVDGKWIEHPPAGEKLKGRGGPGLVVAQG 185
           S+HS AAD+  +Y+FGG     RLN L +Y+ VD KW      G+ L  RGG GL V QG
Sbjct: 271 SFHSMAADEENVYVFGGVSATARLNTLDSYNIVDKKWFHCSTPGDSLTARGGAGLEVVQG 330

Query: 186 KIWVVYGFAGMEIDDVHYFDPAQNVWAQVDTTGEKPTARSVFSTFAIGKYVFIYGGEVDP 245
           K+WVVYGF G E+DDVHY+DP Q+ W QV+T G +P+ RSVF++ AIGK++ I+GGE+  
Sbjct: 331 KVWVVYGFNGCEVDDVHYYDPVQDKWTQVETFGVRPSERSVFASAAIGKHIVIFGGEIAM 390

Query: 246 SDLGHLGAGKFAGEVYVLDTVSLVWSRVEDGLGLENHPGPRGWCAYSVGRKGGELGLVVY 305
             L H+G G+     + LDT +L W R++   G E  P  RGW A +     G+ GLV++
Sbjct: 391 DPLAHVGPGQLTDGTFALDTETLQWERLDKFGGEEETPSSRGWTASTTATIDGKKGLVMH 450

Query: 306 GGNSPSNDRLGDIYFF 321
           GG +P+NDR  D++F+
Sbjct: 451 GGKAPTNDRFDDLFFY 465

BLAST of MC02g0389 vs. TAIR 10
Match: AT3G16400.2 (nitrile specifier protein 1 )

HSP 1 Score: 339.3 bits (869), Expect = 3.3e-93
Identity = 153/316 (48.42%), Postives = 208/316 (65.82%), Query Frame = 0

Query: 6   GKWIKIHQNGTSPGARSSHAITILGQNAYAFGGEFTPRLPVDNKLHVFDLDELTWTVADV 65
           GKWIK+ Q G  PG R SH I  +G   Y+FGGEFTP  P+D  L+VFDL+  TW+++  
Sbjct: 151 GKWIKVEQKGEGPGLRCSHGIAQVGNKIYSFGGEFTPNQPIDKHLYVFDLETRTWSISPA 210

Query: 66  SGDVPP-PRVGVTMAAVGRIVYVFGGRDSDHKELNELYSFDTSINKWTLLSGGDSGPPHR 125
           +GDVP    +GV M +VG  +YVFGGRD+  ++ N  YSFDT+ N+W LL+  + GP  R
Sbjct: 211 TGDVPHLSCLGVRMVSVGSTLYVFGGRDAS-RQYNGFYSFDTTTNEWKLLTPVEEGPTPR 270

Query: 126 SYHSSAADDRRLYIFGGCGNAGRLNDLWAYDTVDGKWIEHPPAGEKLKGRGGPGLVVAQG 185
           S+HS AAD+  +Y+FGG     RLN L +Y+ VD KW      G+ L  RGG GL V QG
Sbjct: 271 SFHSMAADEENVYVFGGVSATARLNTLDSYNIVDKKWFHCSTPGDSLTARGGAGLEVVQG 330

Query: 186 KIWVVYGFAGMEIDDVHYFDPAQNVWAQVDTTGEKPTARSVFSTFAIGKYVFIYGGEVDP 245
           K+WVVYGF G E+DDVHY+DP Q+ W QV+T G +P+ RSVF++ AIGK++ I+GGE+  
Sbjct: 331 KVWVVYGFNGCEVDDVHYYDPVQDKWTQVETFGVRPSERSVFASAAIGKHIVIFGGEIAM 390

Query: 246 SDLGHLGAGKFAGEVYVLDTVSLVWSRVEDGLGLENHPGPRGWCAYSVGRKGGELGLVVY 305
             L H+G G+     + LDT +L W R++   G E  P  RGW A +     G+ GLV++
Sbjct: 391 DPLAHVGPGQLTDGTFALDTETLQWERLDKFGGEEETPSSRGWTASTTATIDGKKGLVMH 450

Query: 306 GGNSPSNDRLGDIYFF 321
           GG +P+NDR  D++F+
Sbjct: 451 GGKAPTNDRFDDLFFY 465