MC01g_new0495 (gene) Bitter gourd (Dali-11) v1

BLAST of MC01g_new0495 vs. ExPASy Swiss-Prot
Match: Q9SVG3 (Berberine bridge enzyme-like 21 OS=Arabidopsis thaliana OX=3702 GN=At4g20840 PE=2 SV=1)

HSP 1 Score: 580.5 bits (1495), Expect = 2.0e-164
Identity = 287/522 (54.98%), Postives = 377/522 (72.22%), Query Frame = 0

Query: 12  FLTLLASMAASHPIPSSQI---LLQCLSDHSSPSSSPISQLVFFPDNPSYSPVLNSYIRN 71
           FL  L   +++ P  S  I    +QC SD +    + I+  VF   NPS+S VL +YIRN
Sbjct: 16  FLVSLPFFSSAAPPSSDSIYESFVQCFSDKTKSPQAQITDNVFSRTNPSFSSVLRAYIRN 75

Query: 72  LRFASPTTPNPLFIVAPTHVSHIQASIICCRIHGLEIRIRSGGHDYDGLSYVSDSPFVIV 131
            RF + +TP P  IV P    H+ A++ C +     ++IRSGGHDY+GLSY+SD PF I+
Sbjct: 76  GRFNTSSTPKPAIIVTPRSDIHVSAAVTCSKSLNFLLKIRSGGHDYEGLSYISDKPFFIL 135

Query: 132 DMFNLRSVAVDIEDESAWVESGATLGEVYFKIAERSKIYGFPAGVCPTVGVGGHLSGAGY 191
           DM NLR V+VDI D+SAW+ +GATLGEVY++I E+SK++GFPAGVCPTVGVGGH+SG GY
Sbjct: 136 DMSNLRDVSVDIADQSAWISAGATLGEVYYRIWEKSKVHGFPAGVCPTVGVGGHISGGGY 195

Query: 192 GNLMRKFGVSVDYVVDALIVDANGRVLDRESMGEDLFWAIRGGGGASFGIIVSWKFKLVP 251
           GN++RKFG+SVD ++DA IVD NG++LDR+SMGEDLFWAI GGGGASFG+++ +K KLVP
Sbjct: 196 GNMLRKFGLSVDNLIDAKIVDVNGQILDRKSMGEDLFWAISGGGGASFGVVLGYKVKLVP 255

Query: 252 LPETVTVFRMEKTMEEGAVDILYKWQQIAHKIDENLFIRVVILPVNKKAKSTAKAKFVSL 311
           +PETVTVFR+EK M+ GAVD+++KWQ +  K D NLF+R++I PV +K   T +A  V+L
Sbjct: 256 VPETVTVFRVEKYMDSGAVDMVHKWQSVGPKTDRNLFLRMLIQPVTRKKVKTVRATVVAL 315

Query: 312 FLGNAEKLFALMSESFPELGVKAEDCKEMSWIESVLFWSNYPNGTSLN--VLLERQPKSE 371
           FLG AE++ AL+ + FPEL +K E+C EM+W +S L+W N  N T ++  V L+R     
Sbjct: 316 FLGRAEEVVALLGKEFPELSLKKENCSEMTWFQSALWWDNRVNPTQIDPKVFLDRNLDRA 375

Query: 372 KFLKKKSDYVQEPISKADLEGMMKKMMELKRPALTFNPYGGKMSQIPESETPFPHRAGNM 431
            F K+KSDYV   I +  +E + KKM EL +  L FNPYGGKM+++  + TPFPHR+  +
Sbjct: 376 NFGKRKSDYVASEIPRDGIESLFKKMTELGKIGLVFNPYGGKMAEVTVNATPFPHRS-KL 435

Query: 432 YKIQYSVTWKEEGDEAAEQNLELIRELYEYMTPHVSKSPRSAYLNYRDVDLGVNGRNGNG 491
           +KIQYSVTW+E   E  +  L     LY +MT  VSK+PR+AYLNYRDVD+GVN  +G  
Sbjct: 436 FKIQYSVTWQENSVEIEKGFLNQANVLYSFMTGFVSKNPRNAYLNYRDVDIGVND-HGTN 495

Query: 492 SYEEASTWGRKYFKGNLERLVEVKTVADPDNFFRYEQSIPSV 529
           SYEE   +GRKYF  N +RLV+VKT ADPDNFFR EQSIP+V
Sbjct: 496 SYEEGEVYGRKYFGDNFDRLVKVKTAADPDNFFRNEQSIPTV 535

BLAST of MC01g_new0495 vs. ExPASy Swiss-Prot
Match: Q9SA85 (Berberine bridge enzyme-like 8 OS=Arabidopsis thaliana OX=3702 GN=At1g30700 PE=2 SV=1)

HSP 1 Score: 569.3 bits (1466), Expect = 4.7e-161
Identity = 273/505 (54.06%), Postives = 373/505 (73.86%), Query Frame = 0

Query: 27  SSQILLQCLSDHSSPSSSPISQLVFFPDNPSYSPVLNSYIRNLRFASPTTPNPLFIVAPT 86
           +S+   QCL+ +S P   PIS  +FF  N SYS VL + IRNLRF + +TP P  I+A T
Sbjct: 24  NSETFTQCLTSNSDP-KHPISPAIFFSGNGSYSSVLQANIRNLRFNTTSTPKPFLIIAAT 83

Query: 87  HVSHIQASIICCRIHGLEIRIRSGGHDYDGLSYV--SDSPFVIVDMFNLRSVAVDIEDES 146
           H SH+QA+I C + H L+++IRSGGHDYDGLSYV  S  PF ++DMFNLRSV VD+  ++
Sbjct: 84  HESHVQAAITCGKRHNLQMKIRSGGHDYDGLSYVTYSGKPFFVLDMFNLRSVDVDVASKT 143

Query: 147 AWVESGATLGEVYFKIAERSKIYGFPAGVCPTVGVGGHLSGAGYGNLMRKFGVSVDYVVD 206
           AWV++GA LGEVY+ I E+SK   +PAG+CPTVGVGGH+SG GYGN+MRK+G++VD  +D
Sbjct: 144 AWVQTGAILGEVYYYIWEKSKTLAYPAGICPTVGVGGHISGGGYGNMMRKYGLTVDNTID 203

Query: 207 ALIVDANGRVLDRESMGEDLFWAIRGGGGASFGIIVSWKFKLVPLPETVTVFRMEKTMEE 266
           A +VD NG++LDR+ MGEDL+WAI GGGG S+G+++++K  LV +PE VTVFR+ +T+E+
Sbjct: 204 ARMVDVNGKILDRKLMGEDLYWAINGGGGGSYGVVLAYKINLVEVPENVTVFRISRTLEQ 263

Query: 267 GAVDILYKWQQIAHKIDENLFIRVVILPVNKKAKS--TAKAKFVSLFLGNAEKLFALMSE 326
            A DI+++WQQ+A K+ + LFIR VI  VN    S  T +  F+++FLG+   L ++++ 
Sbjct: 264 NATDIIHRWQQVAPKLPDELFIRTVIDVVNGTVSSQKTVRTTFIAMFLGDTTTLLSILNR 323

Query: 327 SFPELGVKAEDCKEMSWIESVLFWSNYPNGTSLNVLLERQPKSEKFLKKKSDYVQEPISK 386
            FPELG+   DC E SWI+SVLFW+N   G+S  +LL+R  +   +LK+KSDYV+EPIS+
Sbjct: 324 RFPELGLVRSDCTETSWIQSVLFWTNIQVGSSETLLLQRN-QPVNYLKRKSDYVREPISR 383

Query: 387 ADLEGMMKKMMELKRPALTFNPYGGKMSQIPESETPFPHRAGNMYKIQYSVTWKEEGDEA 446
             LE + KKM+EL+ P + FNPYGG+M +I  + TPFP+RAGN++KIQY   W++E    
Sbjct: 384 TGLESIWKKMIELEIPTMAFNPYGGEMGRISSTVTPFPYRAGNLWKIQYGANWRDE--TL 443

Query: 447 AEQNLELIRELYEYMTPHVSKSPRSAYLNYRDVDLGVNGRNGN-GSYEEASTWGRKYFKG 506
            ++ +EL R+LY++MTP VSK+PR ++ NYRDVDLG+N  NG   SY E   +G+KYF G
Sbjct: 444 TDRYMELTRKLYQFMTPFVSKNPRQSFFNYRDVDLGINSHNGKISSYVEGKRYGKKYFAG 503

Query: 507 NLERLVEVKTVADPDNFFRYEQSIP 527
           N ERLV++KT  D  NFFR EQSIP
Sbjct: 504 NFERLVKIKTRVDSGNFFRNEQSIP 524

BLAST of MC01g_new0495 vs. ExPASy Swiss-Prot
Match: Q9SVG4 (Berberine bridge enzyme-like 19 OS=Arabidopsis thaliana OX=3702 GN=At4g20830 PE=1 SV=2)

HSP 1 Score: 568.2 bits (1463), Expect = 1.0e-160
Identity = 279/521 (53.55%), Postives = 375/521 (71.98%), Query Frame = 0

Query: 11  IFLTL-LASMAASHPIPSSQILLQCLSDHSSPSSSPISQLVFFPDNPSYSPVLNSYIRNL 70
           +FL+L L+S + S+ + +S   L+C SD +    S I+  VF   NP++S VL +YIRN 
Sbjct: 20  LFLSLPLSSFSQSNSVYNS--FLKCFSDKTKSPQSQITDNVFSQTNPAFSSVLRAYIRNA 79

Query: 71  RFASPTTPNPLFIVAPTHVSHIQASIICCRIHGLEIRIRSGGHDYDGLSYVSDSPFVIVD 130
           RF + +T  P  I+ P   SH+ A++ C +     ++IRSGGHDYDGLSY+SD PF I+D
Sbjct: 80  RFNTSSTLKPTIIITPRSESHVSAAVTCSKTLNFLLKIRSGGHDYDGLSYISDKPFFILD 139

Query: 131 MFNLRSVAVDIEDESAWVESGATLGEVYFKIAERSKIYGFPAGVCPTVGVGGHLSGAGYG 190
           M N+R V+VDI   SAW+ +GATLGEVY++I E+S+++GFPAGVCPTVGVGGHLSG GYG
Sbjct: 140 MSNIRDVSVDIASNSAWISAGATLGEVYYRIWEKSRVHGFPAGVCPTVGVGGHLSGGGYG 199

Query: 191 NLMRKFGVSVDYVVDALIVDANGRVLDRESMGEDLFWAIRGGGGASFGIIVSWKFKLVPL 250
           N++RKFG+SVDYV DA IVD NGRVLDR++MGEDLFWAI GGGG S+G+++ +K KLVP+
Sbjct: 200 NMVRKFGLSVDYVEDAKIVDVNGRVLDRKAMGEDLFWAITGGGGGSYGVVLGYKVKLVPV 259

Query: 251 PETVTVFRMEKTMEEGAVDILYKWQQIAHKIDENLFIRVVILPVNKKAKSTAKAKFVSLF 310
           P  VTVFR+E+ M+ GAVD+++KWQ +  K D NLF+R++I PV +K   T +A  V+LF
Sbjct: 260 PSVVTVFRVEQYMDSGAVDMVHKWQSVGPKTDPNLFMRMLIQPVTRKKVKTVRASVVALF 319

Query: 311 LGNAEKLFALMSESFPELGVKAEDCKEMSWIESVLFWSNYPNGTSLN--VLLERQPKSEK 370
           LG A+++ AL+S+ FPELG+K E+C EM+W +S L+W N  N T ++  V L+R   +  
Sbjct: 320 LGRADEVVALLSKEFPELGLKKENCSEMTWFQSALWWDNRLNATQVDPKVFLDRNLDTSS 379

Query: 371 FLKKKSDYVQEPISKADLEGMMKKMMELKRPALTFNPYGGKMSQIPESETPFPHRAGNMY 430
           F K+KSDYV   I K  +E + KKM+EL +  L FNPYGGKM+++  +  PFPHR   ++
Sbjct: 380 FGKRKSDYVATAIPKKGIESLFKKMIELGKIGLVFNPYGGKMAEVAVNAKPFPHR-NKLF 439

Query: 431 KIQYSVTWKEEGDEAAEQNLELIRELYEYMTPHVSKSPRSAYLNYRDVDLGVNGRNGNGS 490
           KIQYSV WKE   E  +  L   + LY +MT  VSK+PRS+Y NYRDVD+GVN    N S
Sbjct: 440 KIQYSVNWKENSAEIEKGYLNQAKVLYSFMTGFVSKNPRSSYFNYRDVDIGVNDHGAN-S 499

Query: 491 YEEASTWGRKYFKGNLERLVEVKTVADPDNFFRYEQSIPSV 529
           Y+E   +GRKYF  N +RLV++KT  DP NFFR EQSIP++
Sbjct: 500 YKEGEVYGRKYFGENFDRLVKIKTAVDPGNFFRNEQSIPTL 536

BLAST of MC01g_new0495 vs. ExPASy Swiss-Prot
Match: F4HV09 (Berberine bridge enzyme-like 14 OS=Arabidopsis thaliana OX=3702 GN=At1g34575 PE=3 SV=1)

HSP 1 Score: 558.5 bits (1438), Expect = 8.2e-158
Identity = 277/523 (52.96%), Postives = 374/523 (71.51%), Query Frame = 0

Query: 8   IIPIFLTLLASMAASHPIPSSQILLQCLSDHSSPSSSPISQLVFFPDNPSYSPVLNSYIR 67
           I  +FL L+ +  A+ P        QC++      S PI    +   NP++  +LN+Y+R
Sbjct: 10  IFTVFLLLIPTSFAAPP-KLKDSFTQCVTVFK--PSVPIQNFTYTQQNPNFLTILNNYVR 69

Query: 68  NLRFASPTTPNPLFIVAPTHVSHIQASIICCRIHGLEIRIRSGGHDYDGLSYVSDSPFVI 127
           NLR+ + TT  P+ IVA  H +HIQA+I C +  GL++RIRSGGHDYDG+SY+S   FV+
Sbjct: 70  NLRYFNGTTRKPVAIVAAAHFTHIQATINCAKKLGLQLRIRSGGHDYDGMSYLSTVDFVV 129

Query: 128 VDMFNLRSVAVDIEDESAWVESGATLGEVYFKIAERS-KIYGFPAGVCPTVGVGGHLSGA 187
           +DMFNLR++ +D + ++AWV+SGATLGE+Y+ +A +S  + GFPAG+CP +G GGH SG 
Sbjct: 130 LDMFNLRAIEIDPKLDTAWVQSGATLGEIYYNVANKSNNLRGFPAGICPGLGAGGHFSGG 189

Query: 188 GYGNLMRKFGVSVDYVVDALIVDANGRVLDRESMGEDLFWAIRGGGGASFGIIVSWKFKL 247
           GYGN+MRK+G+S+D ++DA IVDAN RVLDR SMGEDLFWA+RGGG ASF ++++WK KL
Sbjct: 190 GYGNMMRKYGLSIDNIIDAKIVDANARVLDRSSMGEDLFWALRGGGAASFCVVLAWKIKL 249

Query: 248 VPLPETVTVFRMEKTMEEGAV--DILYKWQQIAHKIDENLFIRVVILPVNKKAKSTAKAK 307
           VP+PE VTVF +E     G +  D+  KWQ+IA KID +LFIR+ +   NK    T KA 
Sbjct: 250 VPVPEKVTVFNVETIGNRGVIPTDLAAKWQEIADKIDNDLFIRLTLSSSNK----TVKAS 309

Query: 308 FVSLFLGNAEKLFALMSESFPELGVKAEDCKEMSWIESVLFWSNYPNGTS-LNVLLERQP 367
           F+ ++LGN+EKL  +M+  FPELG+   +C EM WIESVLFW + P GT+  +V+L R P
Sbjct: 310 FMGMYLGNSEKLLEIMNAKFPELGLNKTECIEMKWIESVLFWLSIPPGTAPTSVMLNRIP 369

Query: 368 KSEKFLKKKSDYVQEPISKADLEGMMKKMMELKRPALTFNPYGGKMSQIPESETPFPHRA 427
           + + +LK+KSDYVQ+PISK  LE + K + E +  ++ +NPYGG+MS+IP +ET FPHRA
Sbjct: 370 QKQIYLKRKSDYVQKPISKPGLESIFKILSENENVSMAWNPYGGRMSEIPATETAFPHRA 429

Query: 428 GNMYKIQYSVTWKEEGDEAAEQNLELIRELYEYMTPHVSKSPRSAYLNYRDVDLGVNGRN 487
           GNM+KIQYS  W   G+EAA   L     ++E M+P+VSK+PR A+LNYRD+D+   G+N
Sbjct: 430 GNMFKIQYSSNWFVPGEEAASDCLSQTERVFEAMSPYVSKNPREAFLNYRDIDI---GKN 489

Query: 488 GNGSYEEASTWGRKYFKGNLERLVEVKTVADPDNFFRYEQSIP 527
            N +YEE   +G KYFK N ERLV+VKT  DPDN FRYEQSIP
Sbjct: 490 LNSTYEEGKVYGVKYFKNNFERLVQVKTRVDPDNIFRYEQSIP 522

BLAST of MC01g_new0495 vs. ExPASy Swiss-Prot
Match: Q9LPC3 (Berberine bridge enzyme-like 1 OS=Arabidopsis thaliana OX=3702 GN=SEC1A PE=2 SV=1)

HSP 1 Score: 551.2 bits (1419), Expect = 1.3e-155
Identity = 268/527 (50.85%), Postives = 377/527 (71.54%), Query Frame = 0

Query: 11  IFLTLLASMAASHPIPSS--QILLQCLSDHSSPSSSPISQLVFFPDNPSYSPVLNSYIRN 70
           I  +L++S  A+ P  +S  +  LQC S+ +      +  +V    + S++P L +YIRN
Sbjct: 10  IVSSLVSSSLATAPPNTSIYESFLQCFSNQTGAPPEKLCDVVLPQSSASFTPTLRAYIRN 69

Query: 71  LRFASPTTPNPLFIVAPTHVSHIQASIICCRIHGLEIRIRSGGHDYDGLSYVSDSPFVIV 130
            RF + T+P PL ++A     H+QA+++C +    +++ RSGGHDYDG+SY+S+ PF ++
Sbjct: 70  ARFNTSTSPKPLLVIAARSECHVQATVLCTKSLNFQLKTRSGGHDYDGVSYISNRPFFVL 129

Query: 131 DMFNLRSVAVDIEDE--SAWVESGATLGEVYFKIAERSK---IYGFPAGVCPTVGVGGHL 190
           DM  LR++ VD+ D+  SAWV +GATLGEVY+ I + SK    +GFPAGVCPTVG GGH+
Sbjct: 130 DMSYLRNITVDMSDDGGSAWVGAGATLGEVYYNIWQSSKTHGTHGFPAGVCPTVGAGGHI 189

Query: 191 SGAGYGNLMRKFGVSVDYVVDALIVDANGRVLDRESMGEDLFWAIRGGGGASFGIIVSWK 250
           SG GYGN++RK+G+SVDYV DA IVD NGR+LDR+SMGEDLFWAI GGGGASFG+I+S+K
Sbjct: 190 SGGGYGNMIRKYGLSVDYVTDAKIVDVNGRILDRKSMGEDLFWAIGGGGGASFGVILSFK 249

Query: 251 FKLVPLPETVTVFRMEKTMEEGAVDILYKWQQIAHKIDENLFIRVVILPVNKKAKSTAKA 310
            KLVP+P  VTVFR+EKT+ E A+D+++KWQ +A K   +LF+R+++ PV +    T +A
Sbjct: 250 IKLVPVPPRVTVFRVEKTLVENALDMVHKWQFVAPKTSPDLFMRLMLQPVTRNTTQTVRA 309

Query: 311 KFVSLFLGNAEKLFALMSESFPELGVKAEDCKEMSWIESVLFWSNYPNGTSL--NVLLER 370
             V+LFLG    L +L+++ FPELG+K E+C EM+WI+SV++W+N  N T +   +LL+R
Sbjct: 310 SVVALFLGKQSDLMSLLTKEFPELGLKPENCTEMTWIQSVMWWANNDNATVIKPEILLDR 369

Query: 371 QPKSEKFLKKKSDYVQEPISKADLEGMMKKMMELKRPALTFNPYGGKMSQIPESETPFPH 430
            P S  FLK+KSDYV++ ISK  L+ + KK+ME  +  L FNPYGGKMS++  + TPFPH
Sbjct: 370 NPDSASFLKRKSDYVEKEISKDGLDFLCKKLMEAGKLGLVFNPYGGKMSEVATTATPFPH 429

Query: 431 RAGNMYKIQYSVTWKEEGDEAAEQNLELIRELYEYMTPHVSKSPRSAYLNYRDVDLGVNG 490
           R   ++K+Q+S+ WK+ G +     +E  R  Y YM P V+K+PR  YLNYRD+D+G+N 
Sbjct: 430 RK-RLFKVQHSMNWKDPGTDVESSFMEKTRSFYSYMAPFVTKNPRHTYLNYRDLDIGINS 489

Query: 491 RNGNGSYEEASTWGRKYFKGNLERLVEVKTVADPDNFFRYEQSIPSV 529
            +G  SY EA  +GRKYF  N +RLV+VKT  DP+NFFR EQSIP++
Sbjct: 490 -HGPNSYREAEVYGRKYFGENFDRLVKVKTAVDPENFFRDEQSIPTL 534

BLAST of MC01g_new0495 vs. NCBI nr
Match: XP_022146727.1 (berberine bridge enzyme-like 21 [Momordica charantia])

HSP 1 Score: 1090 bits (2819), Expect = 0.0
Identity = 549/549 (100.00%), Postives = 549/549 (100.00%), Query Frame = 0

Query: 1   MAIPNSPIIPIFLTLLASMAASHPIPSSQILLQCLSDHSSPSSSPISQLVFFPDNPSYSP 60
           MAIPNSPIIPIFLTLLASMAASHPIPSSQILLQCLSDHSSPSSSPISQLVFFPDNPSYSP
Sbjct: 1   MAIPNSPIIPIFLTLLASMAASHPIPSSQILLQCLSDHSSPSSSPISQLVFFPDNPSYSP 60

Query: 61  VLNSYIRNLRFASPTTPNPLFIVAPTHVSHIQASIICCRIHGLEIRIRSGGHDYDGLSYV 120
           VLNSYIRNLRFASPTTPNPLFIVAPTHVSHIQASIICCRIHGLEIRIRSGGHDYDGLSYV
Sbjct: 61  VLNSYIRNLRFASPTTPNPLFIVAPTHVSHIQASIICCRIHGLEIRIRSGGHDYDGLSYV 120

Query: 121 SDSPFVIVDMFNLRSVAVDIEDESAWVESGATLGEVYFKIAERSKIYGFPAGVCPTVGVG 180
           SDSPFVIVDMFNLRSVAVDIEDESAWVESGATLGEVYFKIAERSKIYGFPAGVCPTVGVG
Sbjct: 121 SDSPFVIVDMFNLRSVAVDIEDESAWVESGATLGEVYFKIAERSKIYGFPAGVCPTVGVG 180

Query: 181 GHLSGAGYGNLMRKFGVSVDYVVDALIVDANGRVLDRESMGEDLFWAIRGGGGASFGIIV 240
           GHLSGAGYGNLMRKFGVSVDYVVDALIVDANGRVLDRESMGEDLFWAIRGGGGASFGIIV
Sbjct: 181 GHLSGAGYGNLMRKFGVSVDYVVDALIVDANGRVLDRESMGEDLFWAIRGGGGASFGIIV 240

Query: 241 SWKFKLVPLPETVTVFRMEKTMEEGAVDILYKWQQIAHKIDENLFIRVVILPVNKKAKST 300
           SWKFKLVPLPETVTVFRMEKTMEEGAVDILYKWQQIAHKIDENLFIRVVILPVNKKAKST
Sbjct: 241 SWKFKLVPLPETVTVFRMEKTMEEGAVDILYKWQQIAHKIDENLFIRVVILPVNKKAKST 300

Query: 301 AKAKFVSLFLGNAEKLFALMSESFPELGVKAEDCKEMSWIESVLFWSNYPNGTSLNVLLE 360
           AKAKFVSLFLGNAEKLFALMSESFPELGVKAEDCKEMSWIESVLFWSNYPNGTSLNVLLE
Sbjct: 301 AKAKFVSLFLGNAEKLFALMSESFPELGVKAEDCKEMSWIESVLFWSNYPNGTSLNVLLE 360

Query: 361 RQPKSEKFLKKKSDYVQEPISKADLEGMMKKMMELKRPALTFNPYGGKMSQIPESETPFP 420
           RQPKSEKFLKKKSDYVQEPISKADLEGMMKKMMELKRPALTFNPYGGKMSQIPESETPFP
Sbjct: 361 RQPKSEKFLKKKSDYVQEPISKADLEGMMKKMMELKRPALTFNPYGGKMSQIPESETPFP 420

Query: 421 HRAGNMYKIQYSVTWKEEGDEAAEQNLELIRELYEYMTPHVSKSPRSAYLNYRDVDLGVN 480
           HRAGNMYKIQYSVTWKEEGDEAAEQNLELIRELYEYMTPHVSKSPRSAYLNYRDVDLGVN
Sbjct: 421 HRAGNMYKIQYSVTWKEEGDEAAEQNLELIRELYEYMTPHVSKSPRSAYLNYRDVDLGVN 480

Query: 481 GRNGNGSYEEASTWGRKYFKGNLERLVEVKTVADPDNFFRYEQSIPSVAFFKNQQSDSNG 540
           GRNGNGSYEEASTWGRKYFKGNLERLVEVKTVADPDNFFRYEQSIPSVAFFKNQQSDSNG
Sbjct: 481 GRNGNGSYEEASTWGRKYFKGNLERLVEVKTVADPDNFFRYEQSIPSVAFFKNQQSDSNG 540

Query: 541 GAVNLKFAI 549
           GAVNLKFAI
Sbjct: 541 GAVNLKFAI 549

BLAST of MC01g_new0495 vs. NCBI nr
Match: XP_022925684.1 (berberine bridge enzyme-like 8 isoform X1 [Cucurbita moschata])

HSP 1 Score: 919 bits (2374), Expect = 0.0
Identity = 465/548 (84.85%), Postives = 501/548 (91.42%), Query Frame = 0

Query: 1   MAIPNSPIIPIFLTLLA---SMAASHPIPSSQILLQCLSDHSSPSSSPISQLVFFPDNPS 60
           MAIP S +IPI  TLL    SMAA+    S   LL CLSDHSSPS+SPIS++ FFPDNPS
Sbjct: 1   MAIPTSLLIPILTTLLLILPSMAAA----SHHTLLSCLSDHSSPSASPISEVTFFPDNPS 60

Query: 61  YSPVLNSYIRNLRFASPTTPNPLFIVAPTHVSHIQASIICCRIHGLEIRIRSGGHDYDGL 120
           YSPVLNSYIRNLRFASPTTP PLFIVAPTH+SHIQASI+CCRIH LE+RIRSGGHDYDGL
Sbjct: 61  YSPVLNSYIRNLRFASPTTPKPLFIVAPTHISHIQASILCCRIHALELRIRSGGHDYDGL 120

Query: 121 SYVSDSPFVIVDMFNLRSVAVDIEDESAWVESGATLGEVYFKIAERSKIYGFPAGVCPTV 180
           SYVSDSPFVI+DMFNLRSVAVDIEDESAWV+SGATLGEVYFKIAE+SKI+GFPAGVCPTV
Sbjct: 121 SYVSDSPFVILDMFNLRSVAVDIEDESAWVDSGATLGEVYFKIAEKSKIHGFPAGVCPTV 180

Query: 181 GVGGHLSGAGYGNLMRKFGVSVDYVVDALIVDANGRVLDRESMGEDLFWAIRGGGGASFG 240
           GVGGHLSGAGYGNLMRKFGVSVD VVDALIVD+NGR LDRESMGEDLFWAIRGGGGASFG
Sbjct: 181 GVGGHLSGAGYGNLMRKFGVSVDNVVDALIVDSNGRALDRESMGEDLFWAIRGGGGASFG 240

Query: 241 IIVSWKFKLVPLPETVTVFRMEKTMEEGAVDILYKWQQIAHKIDENLFIRVVILPVNKKA 300
           +IVSWKF+LVPLPETV+VFRMEKTMEEGAVD+LYKWQ++A KIDENLFIRVVILPVNKK 
Sbjct: 241 VIVSWKFRLVPLPETVSVFRMEKTMEEGAVDMLYKWQEVADKIDENLFIRVVILPVNKKT 300

Query: 301 KSTAKAKFVSLFLGNAEKLFALMSESFPELGVKAEDCKEMSWIESVLFWSNYPNGTSLNV 360
           + TAKAKF+SLFLG+A+KLF+LMSE+ PELGVKAEDC EMSWIESVLFWSNYP GT LNV
Sbjct: 301 QKTAKAKFISLFLGSAQKLFSLMSETLPELGVKAEDCLEMSWIESVLFWSNYPIGTPLNV 360

Query: 361 LLERQPKSEKFLKKKSDYVQEPISKADLEGMMKKMMELKRPALTFNPYGGKMSQIPESET 420
           LLERQP SEKFLKKKSDYVQEPISKADLEGM++KMMELKRPALTFNPYGGKMS+I E ET
Sbjct: 361 LLERQPNSEKFLKKKSDYVQEPISKADLEGMLRKMMELKRPALTFNPYGGKMSEIAERET 420

Query: 421 PFPHRAGNMYKIQYSVTWKEEGDEAAEQNLELIRELYEYMTPHVSKSPRSAYLNYRDVDL 480
           PFPHRAGN YKIQYSVTWKEEG+EAA++N+ELIRELYEYMTPHVSKSPRSAYLNYRDVDL
Sbjct: 421 PFPHRAGNKYKIQYSVTWKEEGEEAADKNVELIRELYEYMTPHVSKSPRSAYLNYRDVDL 480

Query: 481 GVNGRNGNGSYEEASTWGRKYFKGNLERLVEVKTVADPDNFFRYEQSIPSVAFFKNQQSD 540
           GVNG NGN SY EAS WGRKYFKGNL+RLVEVKT+ DP NFFRYEQSIPS A +     D
Sbjct: 481 GVNG-NGNASYWEASHWGRKYFKGNLDRLVEVKTMVDPGNFFRYEQSIPSAAAY-----D 538

Query: 541 SNGGAVNL 545
           SNG A +L
Sbjct: 541 SNGRAESL 538

BLAST of MC01g_new0495 vs. NCBI nr
Match: XP_038883923.1 (berberine bridge enzyme-like 14 [Benincasa hispida])

HSP 1 Score: 916 bits (2368), Expect = 0.0
Identity = 464/548 (84.67%), Postives = 500/548 (91.24%), Query Frame = 0

Query: 1   MAIPNSPIIPI---FLTLLASMAASHPIPSSQILLQCLSDHSSPSSSPISQLVFFPDNPS 60
           M IPNS +IP+   FL +L SMA S  IPS Q LL+CLSDHS+PSSSPIS+++FFPDNPS
Sbjct: 20  MGIPNSLLIPVLTTFLLILPSMAVSDQIPSHQTLLKCLSDHSAPSSSPISEVIFFPDNPS 79

Query: 61  YSPVLNSYIRNLRFASPTTPNPLFIVAPTHVSHIQASIICCRIHGLEIRIRSGGHDYDGL 120
           YSPVLNSYIRNLRFASPTTP PLFIVAPTHVSHIQA+I+CCRIH LEIRIRSGGHDYDGL
Sbjct: 80  YSPVLNSYIRNLRFASPTTPKPLFIVAPTHVSHIQATILCCRIHALEIRIRSGGHDYDGL 139

Query: 121 SYVSDSPFVIVDMFNLRSVAVDIEDESAWVESGATLGEVYFKIAERSKIYGFPAGVCPTV 180
           SYVSDS FVI+DMFNLRSVAVDIEDESAWV+SGATLGEVYFKIAE+SKI+GFPAGVCPTV
Sbjct: 140 SYVSDSTFVILDMFNLRSVAVDIEDESAWVDSGATLGEVYFKIAEKSKIHGFPAGVCPTV 199

Query: 181 GVGGHLSGAGYGNLMRKFGVSVDYVVDALIVDANGRVLDRESMGEDLFWAIRGGGGASFG 240
           GVGGHLSGAGYGNLMRKFGVSVD VVDALIVD NGRVLDR SMGEDLFWAIRGGGGASFG
Sbjct: 200 GVGGHLSGAGYGNLMRKFGVSVDNVVDALIVDVNGRVLDRASMGEDLFWAIRGGGGASFG 259

Query: 241 IIVSWKFKLVPLPETVTVFRMEKTMEEGAVDILYKWQQIAHKIDENLFIRVVILPVNKKA 300
           +IVSWKFKLVPLPETVTVFR EKTMEEG VDILYKWQ+IA KIDENLFIRVVILPVNKKA
Sbjct: 260 VIVSWKFKLVPLPETVTVFRTEKTMEEGVVDILYKWQEIADKIDENLFIRVVILPVNKKA 319

Query: 301 KSTAKAKFVSLFLGNAEKLFALMSESFPELGVKAEDCKEMSWIESVLFWSNYPNGTSLNV 360
             TAKAKF+SLFLGNA+KLFALMS+  PELG+K EDC EMSWI+SVLFWSNYP GT LNV
Sbjct: 320 LKTAKAKFISLFLGNAQKLFALMSKKLPELGIKVEDCIEMSWIDSVLFWSNYPIGTPLNV 379

Query: 361 LLERQPKSEKFLKKKSDYVQEPISKADLEGMMKKMMELKRPALTFNPYGGKMSQIPESET 420
           LLERQPKSEKFLKKKSDYVQEPISKADLEGMM+KM+ELKRPALTFNPYGGKMSQIPE+ET
Sbjct: 380 LLERQPKSEKFLKKKSDYVQEPISKADLEGMMRKMIELKRPALTFNPYGGKMSQIPETET 439

Query: 421 PFPHRAGNMYKIQYSVTWKEEGDEAAEQNLELIRELYEYMTPHVSKSPRSAYLNYRDVDL 480
           PFPHRAGN YKIQYSVTWKEEGDEAA++N+ELIRE+YEYMTP+VSKSPRSAYLNYRDVDL
Sbjct: 440 PFPHRAGNKYKIQYSVTWKEEGDEAADKNVELIREMYEYMTPYVSKSPRSAYLNYRDVDL 499

Query: 481 GVNGRNGNGSYEEASTWGRKYFKGNLERLVEVKTVADPDNFFRYEQSIPSVAFFKNQQSD 540
           GVNG NG+     AS+WGRKYFKGN +RLV+VK++ DPDNFFRYEQSIP V  F     D
Sbjct: 500 GVNG-NGH-----ASSWGRKYFKGNFDRLVKVKSMVDPDNFFRYEQSIPCVEAF-----D 556

Query: 541 SNGGAVNL 545
           SNG A +L
Sbjct: 560 SNGRAESL 556

BLAST of MC01g_new0495 vs. NCBI nr
Match: XP_023544585.1 (berberine bridge enzyme-like 8 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 915 bits (2364), Expect = 0.0
Identity = 465/548 (84.85%), Postives = 497/548 (90.69%), Query Frame = 0

Query: 1   MAIPNSPIIPIFLTLLA---SMAASHPIPSSQILLQCLSDHSSPSSSPISQLVFFPDNPS 60
           M IP S +IPI  TLL    SMAA+    S   LL CLSDHSSPS+SPIS++ FFPDNPS
Sbjct: 1   MEIPTSLLIPILTTLLLILPSMAAA----SHHTLLSCLSDHSSPSASPISEVTFFPDNPS 60

Query: 61  YSPVLNSYIRNLRFASPTTPNPLFIVAPTHVSHIQASIICCRIHGLEIRIRSGGHDYDGL 120
           YSPVLNSYIRNLRFASPTTP PLFIVAPTHVSHIQASI+CCRIH LE+RIRSGGHDYDGL
Sbjct: 61  YSPVLNSYIRNLRFASPTTPKPLFIVAPTHVSHIQASILCCRIHALELRIRSGGHDYDGL 120

Query: 121 SYVSDSPFVIVDMFNLRSVAVDIEDESAWVESGATLGEVYFKIAERSKIYGFPAGVCPTV 180
           SYVSDSPFVI+DMFNLRSVAVDIED SAWV+SGATLGEVYFKIAE+SKI+GFPAGVCPTV
Sbjct: 121 SYVSDSPFVILDMFNLRSVAVDIEDGSAWVDSGATLGEVYFKIAEKSKIHGFPAGVCPTV 180

Query: 181 GVGGHLSGAGYGNLMRKFGVSVDYVVDALIVDANGRVLDRESMGEDLFWAIRGGGGASFG 240
           GVGGHLSGAGYGNLMRKFGVSVD VVDALIVD+NGRVLDRESMGEDLFWAIRGGGGASFG
Sbjct: 181 GVGGHLSGAGYGNLMRKFGVSVDNVVDALIVDSNGRVLDRESMGEDLFWAIRGGGGASFG 240

Query: 241 IIVSWKFKLVPLPETVTVFRMEKTMEEGAVDILYKWQQIAHKIDENLFIRVVILPVNKKA 300
           +IVSWKFKLVPLPETV+VFRMEKTMEEGAVD+LYKWQ++A KIDENLFIRVVILPVNKK 
Sbjct: 241 VIVSWKFKLVPLPETVSVFRMEKTMEEGAVDMLYKWQEVADKIDENLFIRVVILPVNKKT 300

Query: 301 KSTAKAKFVSLFLGNAEKLFALMSESFPELGVKAEDCKEMSWIESVLFWSNYPNGTSLNV 360
           + TAKAKF+SLFLG+A+KLF+LMSE+ PELGVKAEDC EMSWIESVLFWSNYP GT LNV
Sbjct: 301 QKTAKAKFISLFLGSAQKLFSLMSETLPELGVKAEDCLEMSWIESVLFWSNYPTGTPLNV 360

Query: 361 LLERQPKSEKFLKKKSDYVQEPISKADLEGMMKKMMELKRPALTFNPYGGKMSQIPESET 420
           LLERQP SEKFLKKKSDYVQEPISKADLEGMM+KMMELKRPALTFNPYGGKMS+I ESET
Sbjct: 361 LLERQPNSEKFLKKKSDYVQEPISKADLEGMMRKMMELKRPALTFNPYGGKMSEIAESET 420

Query: 421 PFPHRAGNMYKIQYSVTWKEEGDEAAEQNLELIRELYEYMTPHVSKSPRSAYLNYRDVDL 480
           PFPHRAGN YKIQYSVTWKEEG+EAA++N+ LIRELYEYMTPHVSKSPRSAYLNYRDVDL
Sbjct: 421 PFPHRAGNRYKIQYSVTWKEEGEEAADKNVGLIRELYEYMTPHVSKSPRSAYLNYRDVDL 480

Query: 481 GVNGRNGNGSYEEASTWGRKYFKGNLERLVEVKTVADPDNFFRYEQSIPSVAFFKNQQSD 540
           GVNG NGN SY EAS WGRKYFKGN +RLVEVKT+ DP NFFRYEQSIPS A +     D
Sbjct: 481 GVNG-NGNASYWEASHWGRKYFKGNFDRLVEVKTMVDPGNFFRYEQSIPSAAAY-----D 538

Query: 541 SNGGAVNL 545
            NG A  L
Sbjct: 541 CNGRAERL 538

BLAST of MC01g_new0495 vs. NCBI nr
Match: XP_022977474.1 (berberine bridge enzyme-like 8 isoform X1 [Cucurbita maxima])

HSP 1 Score: 912 bits (2357), Expect = 0.0
Identity = 461/548 (84.12%), Postives = 499/548 (91.06%), Query Frame = 0

Query: 1   MAIPNSPIIPIFLTLLA---SMAASHPIPSSQILLQCLSDHSSPSSSPISQLVFFPDNPS 60
           MAIP S +IPI  TLL    SMAA+    S   LL CLSDHSSPS+SPIS++ FFPDNPS
Sbjct: 1   MAIPTSLLIPILTTLLLILPSMAAA----SHHTLLSCLSDHSSPSASPISEVTFFPDNPS 60

Query: 61  YSPVLNSYIRNLRFASPTTPNPLFIVAPTHVSHIQASIICCRIHGLEIRIRSGGHDYDGL 120
           YSPVLNSYIRNLRFASPTTP PLFIVAPTH+SHIQASI+CCRIH LE+RIRSGGHDYDGL
Sbjct: 61  YSPVLNSYIRNLRFASPTTPKPLFIVAPTHISHIQASILCCRIHALELRIRSGGHDYDGL 120

Query: 121 SYVSDSPFVIVDMFNLRSVAVDIEDESAWVESGATLGEVYFKIAERSKIYGFPAGVCPTV 180
           SYVSDSPFVI+DMFNLRSV VDIEDESAWV+SGATLGEVYFKIAE+SKI+GFPAGVCPTV
Sbjct: 121 SYVSDSPFVILDMFNLRSVDVDIEDESAWVDSGATLGEVYFKIAEKSKIHGFPAGVCPTV 180

Query: 181 GVGGHLSGAGYGNLMRKFGVSVDYVVDALIVDANGRVLDRESMGEDLFWAIRGGGGASFG 240
           GVGGHLSGAGYGNLMRKFGVSVD VVDALIVD+NGRVLDRESMGEDLFWAIRGGGGASFG
Sbjct: 181 GVGGHLSGAGYGNLMRKFGVSVDNVVDALIVDSNGRVLDRESMGEDLFWAIRGGGGASFG 240

Query: 241 IIVSWKFKLVPLPETVTVFRMEKTMEEGAVDILYKWQQIAHKIDENLFIRVVILPVNKKA 300
           +IVSWKFKLVPLPETV+VFR+EKTMEEGAVD+LYKWQ++A KIDENLFIRVVILPVNKK 
Sbjct: 241 VIVSWKFKLVPLPETVSVFRIEKTMEEGAVDMLYKWQEVADKIDENLFIRVVILPVNKKT 300

Query: 301 KSTAKAKFVSLFLGNAEKLFALMSESFPELGVKAEDCKEMSWIESVLFWSNYPNGTSLNV 360
           + TAKAKF+SLFLG+A+KL++L+SE+ PELGVKAEDC EMSWIESVLFWSNYP GT LNV
Sbjct: 301 QKTAKAKFISLFLGSAQKLYSLISETLPELGVKAEDCLEMSWIESVLFWSNYPIGTPLNV 360

Query: 361 LLERQPKSEKFLKKKSDYVQEPISKADLEGMMKKMMELKRPALTFNPYGGKMSQIPESET 420
           LLERQP SEKFLKKKSDYVQEPISKADLEGM++KMMELKRPALTFNPYGGKMS+I ESET
Sbjct: 361 LLERQPNSEKFLKKKSDYVQEPISKADLEGMLRKMMELKRPALTFNPYGGKMSEIAESET 420

Query: 421 PFPHRAGNMYKIQYSVTWKEEGDEAAEQNLELIRELYEYMTPHVSKSPRSAYLNYRDVDL 480
           PFPHRAGN YKIQYSVTWKEEG+EAA++N+ELIRELYEYMTPHVSKSPRSAYLNYRDVDL
Sbjct: 421 PFPHRAGNKYKIQYSVTWKEEGEEAADKNVELIRELYEYMTPHVSKSPRSAYLNYRDVDL 480

Query: 481 GVNGRNGNGSYEEASTWGRKYFKGNLERLVEVKTVADPDNFFRYEQSIPSVAFFKNQQSD 540
           GVNG NGN SY EAS WGRKYFKGN +RLVEVKT+ DP NFFRYEQSIPS A +     D
Sbjct: 481 GVNG-NGNASYWEASQWGRKYFKGNFDRLVEVKTMVDPGNFFRYEQSIPSAAAY-----D 538

Query: 541 SNGGAVNL 545
            NG   +L
Sbjct: 541 CNGRGESL 538

BLAST of MC01g_new0495 vs. ExPASy TrEMBL
Match: A0A6J1D0D6 (berberine bridge enzyme-like 21 OS=Momordica charantia OX=3673 GN=LOC111015859 PE=3 SV=1)

HSP 1 Score: 1090 bits (2819), Expect = 0.0
Identity = 549/549 (100.00%), Postives = 549/549 (100.00%), Query Frame = 0

Query: 1   MAIPNSPIIPIFLTLLASMAASHPIPSSQILLQCLSDHSSPSSSPISQLVFFPDNPSYSP 60
           MAIPNSPIIPIFLTLLASMAASHPIPSSQILLQCLSDHSSPSSSPISQLVFFPDNPSYSP
Sbjct: 1   MAIPNSPIIPIFLTLLASMAASHPIPSSQILLQCLSDHSSPSSSPISQLVFFPDNPSYSP 60

Query: 61  VLNSYIRNLRFASPTTPNPLFIVAPTHVSHIQASIICCRIHGLEIRIRSGGHDYDGLSYV 120
           VLNSYIRNLRFASPTTPNPLFIVAPTHVSHIQASIICCRIHGLEIRIRSGGHDYDGLSYV
Sbjct: 61  VLNSYIRNLRFASPTTPNPLFIVAPTHVSHIQASIICCRIHGLEIRIRSGGHDYDGLSYV 120

Query: 121 SDSPFVIVDMFNLRSVAVDIEDESAWVESGATLGEVYFKIAERSKIYGFPAGVCPTVGVG 180
           SDSPFVIVDMFNLRSVAVDIEDESAWVESGATLGEVYFKIAERSKIYGFPAGVCPTVGVG
Sbjct: 121 SDSPFVIVDMFNLRSVAVDIEDESAWVESGATLGEVYFKIAERSKIYGFPAGVCPTVGVG 180

Query: 181 GHLSGAGYGNLMRKFGVSVDYVVDALIVDANGRVLDRESMGEDLFWAIRGGGGASFGIIV 240
           GHLSGAGYGNLMRKFGVSVDYVVDALIVDANGRVLDRESMGEDLFWAIRGGGGASFGIIV
Sbjct: 181 GHLSGAGYGNLMRKFGVSVDYVVDALIVDANGRVLDRESMGEDLFWAIRGGGGASFGIIV 240

Query: 241 SWKFKLVPLPETVTVFRMEKTMEEGAVDILYKWQQIAHKIDENLFIRVVILPVNKKAKST 300
           SWKFKLVPLPETVTVFRMEKTMEEGAVDILYKWQQIAHKIDENLFIRVVILPVNKKAKST
Sbjct: 241 SWKFKLVPLPETVTVFRMEKTMEEGAVDILYKWQQIAHKIDENLFIRVVILPVNKKAKST 300

Query: 301 AKAKFVSLFLGNAEKLFALMSESFPELGVKAEDCKEMSWIESVLFWSNYPNGTSLNVLLE 360
           AKAKFVSLFLGNAEKLFALMSESFPELGVKAEDCKEMSWIESVLFWSNYPNGTSLNVLLE
Sbjct: 301 AKAKFVSLFLGNAEKLFALMSESFPELGVKAEDCKEMSWIESVLFWSNYPNGTSLNVLLE 360

Query: 361 RQPKSEKFLKKKSDYVQEPISKADLEGMMKKMMELKRPALTFNPYGGKMSQIPESETPFP 420
           RQPKSEKFLKKKSDYVQEPISKADLEGMMKKMMELKRPALTFNPYGGKMSQIPESETPFP
Sbjct: 361 RQPKSEKFLKKKSDYVQEPISKADLEGMMKKMMELKRPALTFNPYGGKMSQIPESETPFP 420

Query: 421 HRAGNMYKIQYSVTWKEEGDEAAEQNLELIRELYEYMTPHVSKSPRSAYLNYRDVDLGVN 480
           HRAGNMYKIQYSVTWKEEGDEAAEQNLELIRELYEYMTPHVSKSPRSAYLNYRDVDLGVN
Sbjct: 421 HRAGNMYKIQYSVTWKEEGDEAAEQNLELIRELYEYMTPHVSKSPRSAYLNYRDVDLGVN 480

Query: 481 GRNGNGSYEEASTWGRKYFKGNLERLVEVKTVADPDNFFRYEQSIPSVAFFKNQQSDSNG 540
           GRNGNGSYEEASTWGRKYFKGNLERLVEVKTVADPDNFFRYEQSIPSVAFFKNQQSDSNG
Sbjct: 481 GRNGNGSYEEASTWGRKYFKGNLERLVEVKTVADPDNFFRYEQSIPSVAFFKNQQSDSNG 540

Query: 541 GAVNLKFAI 549
           GAVNLKFAI
Sbjct: 541 GAVNLKFAI 549

BLAST of MC01g_new0495 vs. ExPASy TrEMBL
Match: A0A6J1ECX0 (berberine bridge enzyme-like 8 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111433031 PE=3 SV=1)

HSP 1 Score: 919 bits (2374), Expect = 0.0
Identity = 465/548 (84.85%), Postives = 501/548 (91.42%), Query Frame = 0

Query: 1   MAIPNSPIIPIFLTLLA---SMAASHPIPSSQILLQCLSDHSSPSSSPISQLVFFPDNPS 60
           MAIP S +IPI  TLL    SMAA+    S   LL CLSDHSSPS+SPIS++ FFPDNPS
Sbjct: 1   MAIPTSLLIPILTTLLLILPSMAAA----SHHTLLSCLSDHSSPSASPISEVTFFPDNPS 60

Query: 61  YSPVLNSYIRNLRFASPTTPNPLFIVAPTHVSHIQASIICCRIHGLEIRIRSGGHDYDGL 120
           YSPVLNSYIRNLRFASPTTP PLFIVAPTH+SHIQASI+CCRIH LE+RIRSGGHDYDGL
Sbjct: 61  YSPVLNSYIRNLRFASPTTPKPLFIVAPTHISHIQASILCCRIHALELRIRSGGHDYDGL 120

Query: 121 SYVSDSPFVIVDMFNLRSVAVDIEDESAWVESGATLGEVYFKIAERSKIYGFPAGVCPTV 180
           SYVSDSPFVI+DMFNLRSVAVDIEDESAWV+SGATLGEVYFKIAE+SKI+GFPAGVCPTV
Sbjct: 121 SYVSDSPFVILDMFNLRSVAVDIEDESAWVDSGATLGEVYFKIAEKSKIHGFPAGVCPTV 180

Query: 181 GVGGHLSGAGYGNLMRKFGVSVDYVVDALIVDANGRVLDRESMGEDLFWAIRGGGGASFG 240
           GVGGHLSGAGYGNLMRKFGVSVD VVDALIVD+NGR LDRESMGEDLFWAIRGGGGASFG
Sbjct: 181 GVGGHLSGAGYGNLMRKFGVSVDNVVDALIVDSNGRALDRESMGEDLFWAIRGGGGASFG 240

Query: 241 IIVSWKFKLVPLPETVTVFRMEKTMEEGAVDILYKWQQIAHKIDENLFIRVVILPVNKKA 300
           +IVSWKF+LVPLPETV+VFRMEKTMEEGAVD+LYKWQ++A KIDENLFIRVVILPVNKK 
Sbjct: 241 VIVSWKFRLVPLPETVSVFRMEKTMEEGAVDMLYKWQEVADKIDENLFIRVVILPVNKKT 300

Query: 301 KSTAKAKFVSLFLGNAEKLFALMSESFPELGVKAEDCKEMSWIESVLFWSNYPNGTSLNV 360
           + TAKAKF+SLFLG+A+KLF+LMSE+ PELGVKAEDC EMSWIESVLFWSNYP GT LNV
Sbjct: 301 QKTAKAKFISLFLGSAQKLFSLMSETLPELGVKAEDCLEMSWIESVLFWSNYPIGTPLNV 360

Query: 361 LLERQPKSEKFLKKKSDYVQEPISKADLEGMMKKMMELKRPALTFNPYGGKMSQIPESET 420
           LLERQP SEKFLKKKSDYVQEPISKADLEGM++KMMELKRPALTFNPYGGKMS+I E ET
Sbjct: 361 LLERQPNSEKFLKKKSDYVQEPISKADLEGMLRKMMELKRPALTFNPYGGKMSEIAERET 420

Query: 421 PFPHRAGNMYKIQYSVTWKEEGDEAAEQNLELIRELYEYMTPHVSKSPRSAYLNYRDVDL 480
           PFPHRAGN YKIQYSVTWKEEG+EAA++N+ELIRELYEYMTPHVSKSPRSAYLNYRDVDL
Sbjct: 421 PFPHRAGNKYKIQYSVTWKEEGEEAADKNVELIRELYEYMTPHVSKSPRSAYLNYRDVDL 480

Query: 481 GVNGRNGNGSYEEASTWGRKYFKGNLERLVEVKTVADPDNFFRYEQSIPSVAFFKNQQSD 540
           GVNG NGN SY EAS WGRKYFKGNL+RLVEVKT+ DP NFFRYEQSIPS A +     D
Sbjct: 481 GVNG-NGNASYWEASHWGRKYFKGNLDRLVEVKTMVDPGNFFRYEQSIPSAAAY-----D 538

Query: 541 SNGGAVNL 545
           SNG A +L
Sbjct: 541 SNGRAESL 538

BLAST of MC01g_new0495 vs. ExPASy TrEMBL
Match: A0A6J1IRG6 (berberine bridge enzyme-like 8 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111477807 PE=3 SV=1)

HSP 1 Score: 912 bits (2357), Expect = 0.0
Identity = 461/548 (84.12%), Postives = 499/548 (91.06%), Query Frame = 0

Query: 1   MAIPNSPIIPIFLTLLA---SMAASHPIPSSQILLQCLSDHSSPSSSPISQLVFFPDNPS 60
           MAIP S +IPI  TLL    SMAA+    S   LL CLSDHSSPS+SPIS++ FFPDNPS
Sbjct: 1   MAIPTSLLIPILTTLLLILPSMAAA----SHHTLLSCLSDHSSPSASPISEVTFFPDNPS 60

Query: 61  YSPVLNSYIRNLRFASPTTPNPLFIVAPTHVSHIQASIICCRIHGLEIRIRSGGHDYDGL 120
           YSPVLNSYIRNLRFASPTTP PLFIVAPTH+SHIQASI+CCRIH LE+RIRSGGHDYDGL
Sbjct: 61  YSPVLNSYIRNLRFASPTTPKPLFIVAPTHISHIQASILCCRIHALELRIRSGGHDYDGL 120

Query: 121 SYVSDSPFVIVDMFNLRSVAVDIEDESAWVESGATLGEVYFKIAERSKIYGFPAGVCPTV 180
           SYVSDSPFVI+DMFNLRSV VDIEDESAWV+SGATLGEVYFKIAE+SKI+GFPAGVCPTV
Sbjct: 121 SYVSDSPFVILDMFNLRSVDVDIEDESAWVDSGATLGEVYFKIAEKSKIHGFPAGVCPTV 180

Query: 181 GVGGHLSGAGYGNLMRKFGVSVDYVVDALIVDANGRVLDRESMGEDLFWAIRGGGGASFG 240
           GVGGHLSGAGYGNLMRKFGVSVD VVDALIVD+NGRVLDRESMGEDLFWAIRGGGGASFG
Sbjct: 181 GVGGHLSGAGYGNLMRKFGVSVDNVVDALIVDSNGRVLDRESMGEDLFWAIRGGGGASFG 240

Query: 241 IIVSWKFKLVPLPETVTVFRMEKTMEEGAVDILYKWQQIAHKIDENLFIRVVILPVNKKA 300
           +IVSWKFKLVPLPETV+VFR+EKTMEEGAVD+LYKWQ++A KIDENLFIRVVILPVNKK 
Sbjct: 241 VIVSWKFKLVPLPETVSVFRIEKTMEEGAVDMLYKWQEVADKIDENLFIRVVILPVNKKT 300

Query: 301 KSTAKAKFVSLFLGNAEKLFALMSESFPELGVKAEDCKEMSWIESVLFWSNYPNGTSLNV 360
           + TAKAKF+SLFLG+A+KL++L+SE+ PELGVKAEDC EMSWIESVLFWSNYP GT LNV
Sbjct: 301 QKTAKAKFISLFLGSAQKLYSLISETLPELGVKAEDCLEMSWIESVLFWSNYPIGTPLNV 360

Query: 361 LLERQPKSEKFLKKKSDYVQEPISKADLEGMMKKMMELKRPALTFNPYGGKMSQIPESET 420
           LLERQP SEKFLKKKSDYVQEPISKADLEGM++KMMELKRPALTFNPYGGKMS+I ESET
Sbjct: 361 LLERQPNSEKFLKKKSDYVQEPISKADLEGMLRKMMELKRPALTFNPYGGKMSEIAESET 420

Query: 421 PFPHRAGNMYKIQYSVTWKEEGDEAAEQNLELIRELYEYMTPHVSKSPRSAYLNYRDVDL 480
           PFPHRAGN YKIQYSVTWKEEG+EAA++N+ELIRELYEYMTPHVSKSPRSAYLNYRDVDL
Sbjct: 421 PFPHRAGNKYKIQYSVTWKEEGEEAADKNVELIRELYEYMTPHVSKSPRSAYLNYRDVDL 480

Query: 481 GVNGRNGNGSYEEASTWGRKYFKGNLERLVEVKTVADPDNFFRYEQSIPSVAFFKNQQSD 540
           GVNG NGN SY EAS WGRKYFKGN +RLVEVKT+ DP NFFRYEQSIPS A +     D
Sbjct: 481 GVNG-NGNASYWEASQWGRKYFKGNFDRLVEVKTMVDPGNFFRYEQSIPSAAAY-----D 538

Query: 541 SNGGAVNL 545
            NG   +L
Sbjct: 541 CNGRGESL 538

BLAST of MC01g_new0495 vs. ExPASy TrEMBL
Match: A0A1S3AZM6 (reticuline oxidase-like protein OS=Cucumis melo OX=3656 GN=LOC103484295 PE=3 SV=1)

HSP 1 Score: 899 bits (2324), Expect = 0.0
Identity = 457/547 (83.55%), Postives = 494/547 (90.31%), Query Frame = 0

Query: 3   IPNSPIIPIFLTLL---ASMAASHPIPSSQILLQCLSDHS-SPSSSPISQLVFFPDNPSY 62
           IP+S +IP   TLL   +SMA S   PS Q LL CLSDHS SPSSSPISQ+ FFPDNPSY
Sbjct: 24  IPDSVLIPTLTTLLLILSSMALSAQTPSHQTLLDCLSDHSNSPSSSPISQVTFFPDNPSY 83

Query: 63  SPVLNSYIRNLRFASPTTPNPLFIVAPTHVSHIQASIICCRIHGLEIRIRSGGHDYDGLS 122
           SPVLNSYIRNLRFASPTTP PLFIVAPTHVSHIQA+I+CCRIH LEIRIRSGGHDYDGLS
Sbjct: 84  SPVLNSYIRNLRFASPTTPKPLFIVAPTHVSHIQATILCCRIHALEIRIRSGGHDYDGLS 143

Query: 123 YVSDSPFVIVDMFNLRSVAVDIEDESAWVESGATLGEVYFKIAERSKIYGFPAGVCPTVG 182
           YVSDSPFVI+DMFNLRSVAVDIEDESAWV+SGATLGEVY+KIAE+SKI+GFPAGVCPTVG
Sbjct: 144 YVSDSPFVILDMFNLRSVAVDIEDESAWVDSGATLGEVYYKIAEKSKIHGFPAGVCPTVG 203

Query: 183 VGGHLSGAGYGNLMRKFGVSVDYVVDALIVDANGRVLDRESMGEDLFWAIRGGGGASFGI 242
           VGGHLSGAGYGNLMRKFGVSVD VVDALIVD NGRVLDRE+MGEDLFWAIRGGGGASFG+
Sbjct: 204 VGGHLSGAGYGNLMRKFGVSVDNVVDALIVDVNGRVLDRETMGEDLFWAIRGGGGASFGV 263

Query: 243 IVSWKFKLVPLPETVTVFRMEKTMEEGAVDILYKWQQIAHKIDENLFIRVVILPVNKKAK 302
           IVSWKFKLV LPETVTVFR EKT+EEG VDIL+KWQ+IA  IDENLFIRVVILPVNKK +
Sbjct: 264 IVSWKFKLVLLPETVTVFRTEKTIEEGVVDILHKWQEIADTIDENLFIRVVILPVNKKTQ 323

Query: 303 STAKAKFVSLFLGNAEKLFALMSESFPELGVKAEDCKEMSWIESVLFWSNYPNGTSLNVL 362
            TAKAKFVSLFLGNA+KLFALMSE FPELG+K EDCKEMSWI+S+LFWSNYP GT L+VL
Sbjct: 324 KTAKAKFVSLFLGNAQKLFALMSERFPELGIKGEDCKEMSWIDSILFWSNYPIGTPLDVL 383

Query: 363 LERQPKSEKFLKKKSDYVQEPISKADLEGMMKKMMELKRPALTFNPYGGKMSQIPESETP 422
           LERQP SEKFLKKKSDYVQEPISKA LEGMM+KM+ELKRPALTFNPYGGKMSQIPE+ETP
Sbjct: 384 LERQPNSEKFLKKKSDYVQEPISKAGLEGMMRKMIELKRPALTFNPYGGKMSQIPETETP 443

Query: 423 FPHRAGNMYKIQYSVTWKEEGDEAAEQNLELIRELYEYMTPHVSKSPRSAYLNYRDVDLG 482
           FPHRAGN YKIQYSVTWKEEGDEAA ++LE+IRELY+YMTP+VSKSPRSAYLNYRDVDLG
Sbjct: 444 FPHRAGNKYKIQYSVTWKEEGDEAAAKHLEMIRELYKYMTPYVSKSPRSAYLNYRDVDLG 503

Query: 483 VNGRNGNGSYEEASTWGRKYFKGNLERLVEVKTVADPDNFFRYEQSIPSVAFFKNQQSDS 542
           VNG  GN SY E S WGRKYFKGN +RLV+VK++ DPDNFF+YEQSIP V     +  DS
Sbjct: 504 VNGI-GNASYWEGSIWGRKYFKGNFDRLVKVKSMVDPDNFFKYEQSIPCVEL---EAFDS 563

Query: 543 NGGAVNL 545
           NG A +L
Sbjct: 564 NGRAESL 566

BLAST of MC01g_new0495 vs. ExPASy TrEMBL
Match: A0A5D3CQQ4 (Reticuline oxidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G009800 PE=3 SV=1)

HSP 1 Score: 893 bits (2307), Expect = 0.0
Identity = 448/528 (84.85%), Postives = 483/528 (91.48%), Query Frame = 0

Query: 19  MAASHPIPSSQILLQCLSDHS-SPSSSPISQLVFFPDNPSYSPVLNSYIRNLRFASPTTP 78
           MA S   PS Q LL CLSDHS SPSSSPISQ+ FFPDNPSYSPVLNSYIRNLRFASPTTP
Sbjct: 1   MALSAQTPSHQTLLDCLSDHSNSPSSSPISQVTFFPDNPSYSPVLNSYIRNLRFASPTTP 60

Query: 79  NPLFIVAPTHVSHIQASIICCRIHGLEIRIRSGGHDYDGLSYVSDSPFVIVDMFNLRSVA 138
            PLFIVAPTHVSHIQA+I+CCRIH LEIRIRSGGHDYDGLSYVSDSPFVI+DMFNLRSVA
Sbjct: 61  KPLFIVAPTHVSHIQATILCCRIHALEIRIRSGGHDYDGLSYVSDSPFVILDMFNLRSVA 120

Query: 139 VDIEDESAWVESGATLGEVYFKIAERSKIYGFPAGVCPTVGVGGHLSGAGYGNLMRKFGV 198
           VDI+DESAWV+SGATLGEVY+KIAE+SKI+GFPAGVCPTVGVGGHLSGAGYGNLMRKFGV
Sbjct: 121 VDIKDESAWVDSGATLGEVYYKIAEKSKIHGFPAGVCPTVGVGGHLSGAGYGNLMRKFGV 180

Query: 199 SVDYVVDALIVDANGRVLDRESMGEDLFWAIRGGGGASFGIIVSWKFKLVPLPETVTVFR 258
           SVD VVDALIVD NGRVLDRE+MGEDLFWAIRGGGGASFG+IVSWKFKLV LPETVTVFR
Sbjct: 181 SVDNVVDALIVDVNGRVLDRETMGEDLFWAIRGGGGASFGVIVSWKFKLVLLPETVTVFR 240

Query: 259 MEKTMEEGAVDILYKWQQIAHKIDENLFIRVVILPVNKKAKSTAKAKFVSLFLGNAEKLF 318
            EKT+EEG VDIL+KWQ+IA  IDENLFIRVVILPVNKK + TAKAKFVSLFLGNA+KLF
Sbjct: 241 TEKTIEEGVVDILHKWQEIADTIDENLFIRVVILPVNKKTQKTAKAKFVSLFLGNAQKLF 300

Query: 319 ALMSESFPELGVKAEDCKEMSWIESVLFWSNYPNGTSLNVLLERQPKSEKFLKKKSDYVQ 378
           ALMSE FPELG+K EDCKEMSWI+S+LFWSNYP GT L+VLLERQP SEKFLKKKSDYVQ
Sbjct: 301 ALMSERFPELGIKGEDCKEMSWIDSILFWSNYPIGTPLDVLLERQPNSEKFLKKKSDYVQ 360

Query: 379 EPISKADLEGMMKKMMELKRPALTFNPYGGKMSQIPESETPFPHRAGNMYKIQYSVTWKE 438
           EPISKADLEGMM+KM+ELKRPALTFNPYGGKMSQIPE+ETPFPHRAGN YKIQYSVTWKE
Sbjct: 361 EPISKADLEGMMRKMIELKRPALTFNPYGGKMSQIPETETPFPHRAGNKYKIQYSVTWKE 420

Query: 439 EGDEAAEQNLELIRELYEYMTPHVSKSPRSAYLNYRDVDLGVNGRNGNGSYEEASTWGRK 498
           EGDEAA ++LE+IRELY+YMTP+VSKSPRSAYLNYRDVDLGVNG  GN SY E S WGRK
Sbjct: 421 EGDEAAAKHLEMIRELYKYMTPYVSKSPRSAYLNYRDVDLGVNGI-GNASYWEGSIWGRK 480

Query: 499 YFKGNLERLVEVKTVADPDNFFRYEQSIPSVAFFKNQQSDSNGGAVNL 545
           YFKGN +RLV+VK++ DPDNFF+YEQSIP V     +  DSNG A +L
Sbjct: 481 YFKGNFDRLVKVKSMVDPDNFFKYEQSIPCVEL---EAFDSNGRAKSL 524

BLAST of MC01g_new0495 vs. TAIR 10
Match: AT4G20840.1 (FAD-binding Berberine family protein )

HSP 1 Score: 580.5 bits (1495), Expect = 1.4e-165
Identity = 287/522 (54.98%), Postives = 377/522 (72.22%), Query Frame = 0

Query: 12  FLTLLASMAASHPIPSSQI---LLQCLSDHSSPSSSPISQLVFFPDNPSYSPVLNSYIRN 71
           FL  L   +++ P  S  I    +QC SD +    + I+  VF   NPS+S VL +YIRN
Sbjct: 16  FLVSLPFFSSAAPPSSDSIYESFVQCFSDKTKSPQAQITDNVFSRTNPSFSSVLRAYIRN 75

Query: 72  LRFASPTTPNPLFIVAPTHVSHIQASIICCRIHGLEIRIRSGGHDYDGLSYVSDSPFVIV 131
            RF + +TP P  IV P    H+ A++ C +     ++IRSGGHDY+GLSY+SD PF I+
Sbjct: 76  GRFNTSSTPKPAIIVTPRSDIHVSAAVTCSKSLNFLLKIRSGGHDYEGLSYISDKPFFIL 135

Query: 132 DMFNLRSVAVDIEDESAWVESGATLGEVYFKIAERSKIYGFPAGVCPTVGVGGHLSGAGY 191
           DM NLR V+VDI D+SAW+ +GATLGEVY++I E+SK++GFPAGVCPTVGVGGH+SG GY
Sbjct: 136 DMSNLRDVSVDIADQSAWISAGATLGEVYYRIWEKSKVHGFPAGVCPTVGVGGHISGGGY 195

Query: 192 GNLMRKFGVSVDYVVDALIVDANGRVLDRESMGEDLFWAIRGGGGASFGIIVSWKFKLVP 251
           GN++RKFG+SVD ++DA IVD NG++LDR+SMGEDLFWAI GGGGASFG+++ +K KLVP
Sbjct: 196 GNMLRKFGLSVDNLIDAKIVDVNGQILDRKSMGEDLFWAISGGGGASFGVVLGYKVKLVP 255

Query: 252 LPETVTVFRMEKTMEEGAVDILYKWQQIAHKIDENLFIRVVILPVNKKAKSTAKAKFVSL 311
           +PETVTVFR+EK M+ GAVD+++KWQ +  K D NLF+R++I PV +K   T +A  V+L
Sbjct: 256 VPETVTVFRVEKYMDSGAVDMVHKWQSVGPKTDRNLFLRMLIQPVTRKKVKTVRATVVAL 315

Query: 312 FLGNAEKLFALMSESFPELGVKAEDCKEMSWIESVLFWSNYPNGTSLN--VLLERQPKSE 371
           FLG AE++ AL+ + FPEL +K E+C EM+W +S L+W N  N T ++  V L+R     
Sbjct: 316 FLGRAEEVVALLGKEFPELSLKKENCSEMTWFQSALWWDNRVNPTQIDPKVFLDRNLDRA 375

Query: 372 KFLKKKSDYVQEPISKADLEGMMKKMMELKRPALTFNPYGGKMSQIPESETPFPHRAGNM 431
            F K+KSDYV   I +  +E + KKM EL +  L FNPYGGKM+++  + TPFPHR+  +
Sbjct: 376 NFGKRKSDYVASEIPRDGIESLFKKMTELGKIGLVFNPYGGKMAEVTVNATPFPHRS-KL 435

Query: 432 YKIQYSVTWKEEGDEAAEQNLELIRELYEYMTPHVSKSPRSAYLNYRDVDLGVNGRNGNG 491
           +KIQYSVTW+E   E  +  L     LY +MT  VSK+PR+AYLNYRDVD+GVN  +G  
Sbjct: 436 FKIQYSVTWQENSVEIEKGFLNQANVLYSFMTGFVSKNPRNAYLNYRDVDIGVND-HGTN 495

Query: 492 SYEEASTWGRKYFKGNLERLVEVKTVADPDNFFRYEQSIPSV 529
           SYEE   +GRKYF  N +RLV+VKT ADPDNFFR EQSIP+V
Sbjct: 496 SYEEGEVYGRKYFGDNFDRLVKVKTAADPDNFFRNEQSIPTV 535

BLAST of MC01g_new0495 vs. TAIR 10
Match: AT1G30700.1 (FAD-binding Berberine family protein )

HSP 1 Score: 569.3 bits (1466), Expect = 3.3e-162
Identity = 273/505 (54.06%), Postives = 373/505 (73.86%), Query Frame = 0

Query: 27  SSQILLQCLSDHSSPSSSPISQLVFFPDNPSYSPVLNSYIRNLRFASPTTPNPLFIVAPT 86
           +S+   QCL+ +S P   PIS  +FF  N SYS VL + IRNLRF + +TP P  I+A T
Sbjct: 24  NSETFTQCLTSNSDP-KHPISPAIFFSGNGSYSSVLQANIRNLRFNTTSTPKPFLIIAAT 83

Query: 87  HVSHIQASIICCRIHGLEIRIRSGGHDYDGLSYV--SDSPFVIVDMFNLRSVAVDIEDES 146
           H SH+QA+I C + H L+++IRSGGHDYDGLSYV  S  PF ++DMFNLRSV VD+  ++
Sbjct: 84  HESHVQAAITCGKRHNLQMKIRSGGHDYDGLSYVTYSGKPFFVLDMFNLRSVDVDVASKT 143

Query: 147 AWVESGATLGEVYFKIAERSKIYGFPAGVCPTVGVGGHLSGAGYGNLMRKFGVSVDYVVD 206
           AWV++GA LGEVY+ I E+SK   +PAG+CPTVGVGGH+SG GYGN+MRK+G++VD  +D
Sbjct: 144 AWVQTGAILGEVYYYIWEKSKTLAYPAGICPTVGVGGHISGGGYGNMMRKYGLTVDNTID 203

Query: 207 ALIVDANGRVLDRESMGEDLFWAIRGGGGASFGIIVSWKFKLVPLPETVTVFRMEKTMEE 266
           A +VD NG++LDR+ MGEDL+WAI GGGG S+G+++++K  LV +PE VTVFR+ +T+E+
Sbjct: 204 ARMVDVNGKILDRKLMGEDLYWAINGGGGGSYGVVLAYKINLVEVPENVTVFRISRTLEQ 263

Query: 267 GAVDILYKWQQIAHKIDENLFIRVVILPVNKKAKS--TAKAKFVSLFLGNAEKLFALMSE 326
            A DI+++WQQ+A K+ + LFIR VI  VN    S  T +  F+++FLG+   L ++++ 
Sbjct: 264 NATDIIHRWQQVAPKLPDELFIRTVIDVVNGTVSSQKTVRTTFIAMFLGDTTTLLSILNR 323

Query: 327 SFPELGVKAEDCKEMSWIESVLFWSNYPNGTSLNVLLERQPKSEKFLKKKSDYVQEPISK 386
            FPELG+   DC E SWI+SVLFW+N   G+S  +LL+R  +   +LK+KSDYV+EPIS+
Sbjct: 324 RFPELGLVRSDCTETSWIQSVLFWTNIQVGSSETLLLQRN-QPVNYLKRKSDYVREPISR 383

Query: 387 ADLEGMMKKMMELKRPALTFNPYGGKMSQIPESETPFPHRAGNMYKIQYSVTWKEEGDEA 446
             LE + KKM+EL+ P + FNPYGG+M +I  + TPFP+RAGN++KIQY   W++E    
Sbjct: 384 TGLESIWKKMIELEIPTMAFNPYGGEMGRISSTVTPFPYRAGNLWKIQYGANWRDE--TL 443

Query: 447 AEQNLELIRELYEYMTPHVSKSPRSAYLNYRDVDLGVNGRNGN-GSYEEASTWGRKYFKG 506
            ++ +EL R+LY++MTP VSK+PR ++ NYRDVDLG+N  NG   SY E   +G+KYF G
Sbjct: 444 TDRYMELTRKLYQFMTPFVSKNPRQSFFNYRDVDLGINSHNGKISSYVEGKRYGKKYFAG 503

Query: 507 NLERLVEVKTVADPDNFFRYEQSIP 527
           N ERLV++KT  D  NFFR EQSIP
Sbjct: 504 NFERLVKIKTRVDSGNFFRNEQSIP 524

BLAST of MC01g_new0495 vs. TAIR 10
Match: AT4G20830.1 (FAD-binding Berberine family protein )

HSP 1 Score: 568.2 bits (1463), Expect = 7.4e-162
Identity = 279/521 (53.55%), Postives = 375/521 (71.98%), Query Frame = 0

Query: 11  IFLTL-LASMAASHPIPSSQILLQCLSDHSSPSSSPISQLVFFPDNPSYSPVLNSYIRNL 70
           +FL+L L+S + S+ + +S   L+C SD +    S I+  VF   NP++S VL +YIRN 
Sbjct: 20  LFLSLPLSSFSQSNSVYNS--FLKCFSDKTKSPQSQITDNVFSQTNPAFSSVLRAYIRNA 79

Query: 71  RFASPTTPNPLFIVAPTHVSHIQASIICCRIHGLEIRIRSGGHDYDGLSYVSDSPFVIVD 130
           RF + +T  P  I+ P   SH+ A++ C +     ++IRSGGHDYDGLSY+SD PF I+D
Sbjct: 80  RFNTSSTLKPTIIITPRSESHVSAAVTCSKTLNFLLKIRSGGHDYDGLSYISDKPFFILD 139

Query: 131 MFNLRSVAVDIEDESAWVESGATLGEVYFKIAERSKIYGFPAGVCPTVGVGGHLSGAGYG 190
           M N+R V+VDI   SAW+ +GATLGEVY++I E+S+++GFPAGVCPTVGVGGHLSG GYG
Sbjct: 140 MSNIRDVSVDIASNSAWISAGATLGEVYYRIWEKSRVHGFPAGVCPTVGVGGHLSGGGYG 199

Query: 191 NLMRKFGVSVDYVVDALIVDANGRVLDRESMGEDLFWAIRGGGGASFGIIVSWKFKLVPL 250
           N++RKFG+SVDYV DA IVD NGRVLDR++MGEDLFWAI GGGG S+G+++ +K KLVP+
Sbjct: 200 NMVRKFGLSVDYVEDAKIVDVNGRVLDRKAMGEDLFWAITGGGGGSYGVVLGYKVKLVPV 259

Query: 251 PETVTVFRMEKTMEEGAVDILYKWQQIAHKIDENLFIRVVILPVNKKAKSTAKAKFVSLF 310
           P  VTVFR+E+ M+ GAVD+++KWQ +  K D NLF+R++I PV +K   T +A  V+LF
Sbjct: 260 PSVVTVFRVEQYMDSGAVDMVHKWQSVGPKTDPNLFMRMLIQPVTRKKVKTVRASVVALF 319

Query: 311 LGNAEKLFALMSESFPELGVKAEDCKEMSWIESVLFWSNYPNGTSLN--VLLERQPKSEK 370
           LG A+++ AL+S+ FPELG+K E+C EM+W +S L+W N  N T ++  V L+R   +  
Sbjct: 320 LGRADEVVALLSKEFPELGLKKENCSEMTWFQSALWWDNRLNATQVDPKVFLDRNLDTSS 379

Query: 371 FLKKKSDYVQEPISKADLEGMMKKMMELKRPALTFNPYGGKMSQIPESETPFPHRAGNMY 430
           F K+KSDYV   I K  +E + KKM+EL +  L FNPYGGKM+++  +  PFPHR   ++
Sbjct: 380 FGKRKSDYVATAIPKKGIESLFKKMIELGKIGLVFNPYGGKMAEVAVNAKPFPHR-NKLF 439

Query: 431 KIQYSVTWKEEGDEAAEQNLELIRELYEYMTPHVSKSPRSAYLNYRDVDLGVNGRNGNGS 490
           KIQYSV WKE   E  +  L   + LY +MT  VSK+PRS+Y NYRDVD+GVN    N S
Sbjct: 440 KIQYSVNWKENSAEIEKGYLNQAKVLYSFMTGFVSKNPRSSYFNYRDVDIGVNDHGAN-S 499

Query: 491 YEEASTWGRKYFKGNLERLVEVKTVADPDNFFRYEQSIPSV 529
           Y+E   +GRKYF  N +RLV++KT  DP NFFR EQSIP++
Sbjct: 500 YKEGEVYGRKYFGENFDRLVKIKTAVDPGNFFRNEQSIPTL 536

BLAST of MC01g_new0495 vs. TAIR 10
Match: AT4G20830.2 (FAD-binding Berberine family protein )

HSP 1 Score: 568.2 bits (1463), Expect = 7.4e-162
Identity = 279/521 (53.55%), Postives = 375/521 (71.98%), Query Frame = 0

Query: 11  IFLTL-LASMAASHPIPSSQILLQCLSDHSSPSSSPISQLVFFPDNPSYSPVLNSYIRNL 70
           +FL+L L+S + S+ + +S   L+C SD +    S I+  VF   NP++S VL +YIRN 
Sbjct: 20  LFLSLPLSSFSQSNSVYNS--FLKCFSDKTKSPQSQITDNVFSQTNPAFSSVLRAYIRNA 79

Query: 71  RFASPTTPNPLFIVAPTHVSHIQASIICCRIHGLEIRIRSGGHDYDGLSYVSDSPFVIVD 130
           RF + +T  P  I+ P   SH+ A++ C +     ++IRSGGHDYDGLSY+SD PF I+D
Sbjct: 80  RFNTSSTLKPTIIITPRSESHVSAAVTCSKTLNFLLKIRSGGHDYDGLSYISDKPFFILD 139

Query: 131 MFNLRSVAVDIEDESAWVESGATLGEVYFKIAERSKIYGFPAGVCPTVGVGGHLSGAGYG 190
           M N+R V+VDI   SAW+ +GATLGEVY++I E+S+++GFPAGVCPTVGVGGHLSG GYG
Sbjct: 140 MSNIRDVSVDIASNSAWISAGATLGEVYYRIWEKSRVHGFPAGVCPTVGVGGHLSGGGYG 199

Query: 191 NLMRKFGVSVDYVVDALIVDANGRVLDRESMGEDLFWAIRGGGGASFGIIVSWKFKLVPL 250
           N++RKFG+SVDYV DA IVD NGRVLDR++MGEDLFWAI GGGG S+G+++ +K KLVP+
Sbjct: 200 NMVRKFGLSVDYVEDAKIVDVNGRVLDRKAMGEDLFWAITGGGGGSYGVVLGYKVKLVPV 259

Query: 251 PETVTVFRMEKTMEEGAVDILYKWQQIAHKIDENLFIRVVILPVNKKAKSTAKAKFVSLF 310
           P  VTVFR+E+ M+ GAVD+++KWQ +  K D NLF+R++I PV +K   T +A  V+LF
Sbjct: 260 PSVVTVFRVEQYMDSGAVDMVHKWQSVGPKTDPNLFMRMLIQPVTRKKVKTVRASVVALF 319

Query: 311 LGNAEKLFALMSESFPELGVKAEDCKEMSWIESVLFWSNYPNGTSLN--VLLERQPKSEK 370
           LG A+++ AL+S+ FPELG+K E+C EM+W +S L+W N  N T ++  V L+R   +  
Sbjct: 320 LGRADEVVALLSKEFPELGLKKENCSEMTWFQSALWWDNRLNATQVDPKVFLDRNLDTSS 379

Query: 371 FLKKKSDYVQEPISKADLEGMMKKMMELKRPALTFNPYGGKMSQIPESETPFPHRAGNMY 430
           F K+KSDYV   I K  +E + KKM+EL +  L FNPYGGKM+++  +  PFPHR   ++
Sbjct: 380 FGKRKSDYVATAIPKKGIESLFKKMIELGKIGLVFNPYGGKMAEVAVNAKPFPHR-NKLF 439

Query: 431 KIQYSVTWKEEGDEAAEQNLELIRELYEYMTPHVSKSPRSAYLNYRDVDLGVNGRNGNGS 490
           KIQYSV WKE   E  +  L   + LY +MT  VSK+PRS+Y NYRDVD+GVN    N S
Sbjct: 440 KIQYSVNWKENSAEIEKGYLNQAKVLYSFMTGFVSKNPRSSYFNYRDVDIGVNDHGAN-S 499

Query: 491 YEEASTWGRKYFKGNLERLVEVKTVADPDNFFRYEQSIPSV 529
           Y+E   +GRKYF  N +RLV++KT  DP NFFR EQSIP++
Sbjct: 500 YKEGEVYGRKYFGENFDRLVKIKTAVDPGNFFRNEQSIPTL 536

BLAST of MC01g_new0495 vs. TAIR 10
Match: AT1G34575.1 (FAD-binding Berberine family protein )

HSP 1 Score: 558.5 bits (1438), Expect = 5.9e-159
Identity = 277/523 (52.96%), Postives = 374/523 (71.51%), Query Frame = 0

Query: 8   IIPIFLTLLASMAASHPIPSSQILLQCLSDHSSPSSSPISQLVFFPDNPSYSPVLNSYIR 67
           I  +FL L+ +  A+ P        QC++      S PI    +   NP++  +LN+Y+R
Sbjct: 10  IFTVFLLLIPTSFAAPP-KLKDSFTQCVTVFK--PSVPIQNFTYTQQNPNFLTILNNYVR 69

Query: 68  NLRFASPTTPNPLFIVAPTHVSHIQASIICCRIHGLEIRIRSGGHDYDGLSYVSDSPFVI 127
           NLR+ + TT  P+ IVA  H +HIQA+I C +  GL++RIRSGGHDYDG+SY+S   FV+
Sbjct: 70  NLRYFNGTTRKPVAIVAAAHFTHIQATINCAKKLGLQLRIRSGGHDYDGMSYLSTVDFVV 129

Query: 128 VDMFNLRSVAVDIEDESAWVESGATLGEVYFKIAERS-KIYGFPAGVCPTVGVGGHLSGA 187
           +DMFNLR++ +D + ++AWV+SGATLGE+Y+ +A +S  + GFPAG+CP +G GGH SG 
Sbjct: 130 LDMFNLRAIEIDPKLDTAWVQSGATLGEIYYNVANKSNNLRGFPAGICPGLGAGGHFSGG 189

Query: 188 GYGNLMRKFGVSVDYVVDALIVDANGRVLDRESMGEDLFWAIRGGGGASFGIIVSWKFKL 247
           GYGN+MRK+G+S+D ++DA IVDAN RVLDR SMGEDLFWA+RGGG ASF ++++WK KL
Sbjct: 190 GYGNMMRKYGLSIDNIIDAKIVDANARVLDRSSMGEDLFWALRGGGAASFCVVLAWKIKL 249

Query: 248 VPLPETVTVFRMEKTMEEGAV--DILYKWQQIAHKIDENLFIRVVILPVNKKAKSTAKAK 307
           VP+PE VTVF +E     G +  D+  KWQ+IA KID +LFIR+ +   NK    T KA 
Sbjct: 250 VPVPEKVTVFNVETIGNRGVIPTDLAAKWQEIADKIDNDLFIRLTLSSSNK----TVKAS 309

Query: 308 FVSLFLGNAEKLFALMSESFPELGVKAEDCKEMSWIESVLFWSNYPNGTS-LNVLLERQP 367
           F+ ++LGN+EKL  +M+  FPELG+   +C EM WIESVLFW + P GT+  +V+L R P
Sbjct: 310 FMGMYLGNSEKLLEIMNAKFPELGLNKTECIEMKWIESVLFWLSIPPGTAPTSVMLNRIP 369

Query: 368 KSEKFLKKKSDYVQEPISKADLEGMMKKMMELKRPALTFNPYGGKMSQIPESETPFPHRA 427
           + + +LK+KSDYVQ+PISK  LE + K + E +  ++ +NPYGG+MS+IP +ET FPHRA
Sbjct: 370 QKQIYLKRKSDYVQKPISKPGLESIFKILSENENVSMAWNPYGGRMSEIPATETAFPHRA 429

Query: 428 GNMYKIQYSVTWKEEGDEAAEQNLELIRELYEYMTPHVSKSPRSAYLNYRDVDLGVNGRN 487
           GNM+KIQYS  W   G+EAA   L     ++E M+P+VSK+PR A+LNYRD+D+   G+N
Sbjct: 430 GNMFKIQYSSNWFVPGEEAASDCLSQTERVFEAMSPYVSKNPREAFLNYRDIDI---GKN 489

Query: 488 GNGSYEEASTWGRKYFKGNLERLVEVKTVADPDNFFRYEQSIP 527
            N +YEE   +G KYFK N ERLV+VKT  DPDN FRYEQSIP
Sbjct: 490 LNSTYEEGKVYGVKYFKNNFERLVQVKTRVDPDNIFRYEQSIP 522