MC01g_new0383 (gene) Bitter gourd (Dali-11) v1

BLAST of MC01g_new0383 vs. ExPASy Swiss-Prot
Match: A0A068Q5V6 (Cytochrome P450 71AU50 OS=Prunus mume OX=102107 GN=CYP71AU50 PE=2 SV=1)

HSP 1 Score: 659.1 bits (1699), Expect = 4.0e-188
Identity = 315/492 (64.02%), Postives = 393/492 (79.88%), Query Frame = 0

Query: 4   VWLIITILPLFLLFSSWLWVLRSHARLPPGPKGFPIFGSLHLLGKLPHRALHRLSQKYGP 63
           VW+  TI  L L+     W      RLPPGP+GFPIFGSLHLLG+ P++ LHRL++KYG 
Sbjct: 2   VWIWATIGLLALVHILQAWWKNKKKRLPPGPRGFPIFGSLHLLGEFPNKDLHRLARKYGD 61

Query: 64  IMHLRLGLVSTIVVSSPAAAELFLKTHDHVFASRPLIQASNHISYGRKGLGFAEYGPYWR 123
           IM++RLGL+ TIV+SSP AAELFLKTHD VFASRP  + S HIS+G+K L F+EYG YWR
Sbjct: 62  IMYMRLGLMPTIVISSPEAAELFLKTHDLVFASRPPHEGSKHISFGQKNLIFSEYGAYWR 121

Query: 124 NIRKMCTVELLSNLKVSSFRSMRSEEVGLLIDHLREAAKS-DVVVNLSSKVTSLVADMTC 183
           + RKMCT+ELLSN K++SF+SMR EEV L ++ +R AA +  V V+LS KV+SL  DM+C
Sbjct: 122 DTRKMCTIELLSNHKINSFKSMRREEVSLCVESIRAAANNRGVAVDLSDKVSSLSVDMSC 181

Query: 184 LMVFGKKYGDEQLDERGFKAVIQEVMQLAAAPNLGDFIPYVAGLDFQGFNRRAKSISKVI 243
            MV GKKY DE+ DERGFK+V++E +QLA+APNLGD+I ++A LD QGF +R KS++K  
Sbjct: 182 RMVLGKKYRDEEFDERGFKSVVREAIQLASAPNLGDYIRFIAPLDLQGFTKRMKSVNKAF 241

Query: 244 DGFLERIIDEHLQPKIGNKTKDFVDVMLDLMNSQAREYQIERSSIKAIILDLLAAAVDTS 303
           D   E+II+EHLQP  G +T DFVDVM+  M S+  EY+IER  IKAI+LD+L A++DTS
Sbjct: 242 DNLFEKIIEEHLQPNDGERTMDFVDVMVGFMGSEESEYRIERPHIKAIMLDMLVASMDTS 301

Query: 304 ATTISWALSELIKHPLSMKKIQDELEKVVGLDRMVEDSDLCNLKYLDMVIKEIFRMHPPA 363
           ATTI WALSEL++HP +MKK+Q ELE VVGLD+MVE+SDL  L YL+MV+KE FR+HP A
Sbjct: 302 ATTIEWALSELMRHPKAMKKVQKELENVVGLDKMVEESDLEKLDYLNMVVKETFRLHPVA 361

Query: 364 PLLVPHESLDDCTVDNFYIPKKSRIIVNAWAIGRDPNVWIDAEKFFPERFIGSQVDIKGR 423
           PLL+PH S++DCTV+ ++IPKKSR+++N WAIGRDPN W DAEKF PERF GS VD++G 
Sbjct: 362 PLLIPHASIEDCTVNGYHIPKKSRVLINVWAIGRDPNAWTDAEKFIPERFEGSSVDVRGN 421

Query: 424 DFQLIPFGSGRRGCPGMQMGLIVVRLVLAQLVHCFNWELPNGTLPTKLNMTEEFGLTCPR 483
            FQLIPFGSGRR CPG+Q+GL VV+LVLAQLVHCF+WELPN  LP +L+MTEEFGLT PR
Sbjct: 422 HFQLIPFGSGRRRCPGIQLGLTVVQLVLAQLVHCFDWELPNNMLPEELDMTEEFGLTVPR 481

Query: 484 AEDLKVIPIYRI 495
           A+ L  IP YR+
Sbjct: 482 AKHLLAIPSYRL 493

BLAST of MC01g_new0383 vs. ExPASy Swiss-Prot
Match: H2DH18 (Cytochrome P450 CYP736A12 OS=Panax ginseng OX=4054 PE=2 SV=1)

HSP 1 Score: 553.1 bits (1424), Expect = 3.1e-156
Identity = 268/496 (54.03%), Postives = 363/496 (73.19%), Query Frame = 0

Query: 13  LFLLFSSWLW-------VLRSHARLPPGPKGFPIFGSLHLLGKLPHRALHRLSQKYGPIM 72
           LF+L  +  W        L+ H +LPPGP+G PI GSLH LG LPHR L  L++KYGPIM
Sbjct: 9   LFVLLGALSWWILPIISPLKRHHKLPPGPRGLPIIGSLHTLGALPHRTLQTLAKKYGPIM 68

Query: 73  HLRLGLVSTIVVSSPAAAELFLKTHDHVFASRPLIQASNHISYGRKGLGFAEYGPYWRNI 132
            +RLG V TIVVSSP AAELFLKTHD++FASRP +QA+ ++SYG  G+ F  YGP+WRNI
Sbjct: 69  SMRLGSVPTIVVSSPQAAELFLKTHDNIFASRPKLQAAEYMSYGTMGMSFTAYGPHWRNI 128

Query: 133 RKMCTVELLSNLKVSSFRSMRSEEVGLLIDHLREAAKSDVVVNLSSKVTSLVADMTCLMV 192
           RK   +ELL+  K++SF  MR EE+G ++  ++EA+ ++ VV+LS+KV +++ +MT  ++
Sbjct: 129 RKFVVLELLTPAKINSFVGMRREELGTVVKSIKEASAANEVVDLSAKVANIIENMTYRLL 188

Query: 193 FGKKYGDEQLDERGFKAVIQEVMQLAAAPNLGDFIPYVAGLDFQGFNRRAKSISKVIDGF 252
            G+   D        K ++ E + LA   N+ DF+P++  LD QG  R+ K   K +D  
Sbjct: 189 LGRTKDDRY----DLKGIMNEALTLAGRFNIADFVPFLGPLDIQGLTRQFKDTGKRLDKI 248

Query: 253 LERIIDEHLQ-PKIGNKTKDFVDVMLDLMNSQAREYQ-----IERSSIKAIILDLLAAAV 312
           LE IIDEH Q    GN + DF+D ML L N  +  +      I+RS IKAI++D+++AA+
Sbjct: 249 LEFIIDEHEQNSSNGNASGDFIDDMLSLKNKPSNTHDELSKVIDRSVIKAIMIDIISAAI 308

Query: 313 DTSATTISWALSELIKHPLSMKKIQDELEKVVGLDRMVEDSDLCNLKYLDMVIKEIFRMH 372
           DTS T+I W L+ELIKHP +MKK Q+E++ VVG+DRMVE++DL NL+Y+ MV+KE  R+H
Sbjct: 309 DTSDTSIEWILTELIKHPRAMKKCQEEIDAVVGVDRMVEETDLPNLEYVYMVVKEGLRLH 368

Query: 373 PPAPLLVPHESLDDCTVDNFYIPKKSRIIVNAWAIGRDPNVWID-AEKFFPERFIGSQVD 432
           P APLL PHES++D T++ ++IPK+SR+IVN+WA+GRDPNVW + AE+F PERF GS VD
Sbjct: 369 PVAPLLGPHESMEDITINGYFIPKQSRVIVNSWALGRDPNVWSENAEEFLPERFEGSNVD 428

Query: 433 IKGRDFQLIPFGSGRRGCPGMQMGLIVVRLVLAQLVHCFNWELPNGTLPTKLNMTEEFGL 492
           ++GRDFQL+PFGSGRRGCPGMQ+GLI V+LV+A+LVHCF+W LPNGT P  L+MTE+FGL
Sbjct: 429 VRGRDFQLLPFGSGRRGCPGMQLGLITVQLVVARLVHCFDWNLPNGTTPDNLDMTEKFGL 488

Query: 493 TCPRAEDLKVIPIYRI 495
           T PR + L  +P YR+
Sbjct: 489 TTPRVKHLLAVPKYRL 500

BLAST of MC01g_new0383 vs. ExPASy Swiss-Prot
Match: P24465 (Cytochrome P450 71A1 OS=Persea americana OX=3435 GN=CYP71A1 PE=1 SV=2)

HSP 1 Score: 412.5 bits (1059), Expect = 6.6e-114
Identity = 208/486 (42.80%), Postives = 302/486 (62.14%), Query Frame = 0

Query: 6   LIITILPLFLLFSSWLWVLRSHAR------LPPGPKGFPIFGSLHLLGKLPHRALHRLSQ 65
           + I +  LFL  +   ++L+ + +      LPP P   PI G+LH LG LPHR+L  L+ 
Sbjct: 1   MAILVSLLFLAIALTFFLLKLNEKREKKPNLPPSPPNLPIIGNLHQLGNLPHRSLRSLAN 60

Query: 66  KYGPIMHLRLGLVSTIVVSSPAAAELFLKTHDHVFASRPLIQASNHISYGRKGLGFAEYG 125
           + GP++ L LG + T++VS+   AE  LKTHD +FASRP   A+  I Y    + F+ YG
Sbjct: 61  ELGPLILLHLGHIPTLIVSTAEIAEEILKTHDLIFASRPSTTAARRIFYDCTDVAFSPYG 120

Query: 126 PYWRNIRKMCTVELLSNLKVSSFRSMRSEEVGLLIDHLREAAKSDVVVNLSSKVTSLVAD 185
            YWR +RK+C +ELLS  +V+S+RS+R EEVGL+++ + ++  +   VNLS  +  L + 
Sbjct: 121 EYWRQVRKICVLELLSIKRVNSYRSIREEEVGLMMERISQSCSTGEAVNLSELLLLLSSG 180

Query: 186 MTCLMVFGKKYGDEQLDERGFKAVIQEVMQLAAAPNLGDFIPYVAGLD-FQGFNRRAKSI 245
               + FGKKY  E+  +  F  +  E+  L  A  +GD+ P  A +D   G + R K  
Sbjct: 181 TITRVAFGKKYEGEEERKNKFADLATELTTLMGAFFVGDYFPSFAWVDVLTGMDARLKRN 240

Query: 246 SKVIDGFLERIIDEHLQPKIGN-----KTKDFVDVMLDLMNSQAREYQIERSSIKAIILD 305
              +D F++ +ID+HL  +  N     + KD VDV+L L    +    + R+++KA+ILD
Sbjct: 241 HGELDAFVDHVIDDHLLSRKANGSDGVEQKDLVDVLLHLQKDSSLGVHLNRNNLKAVILD 300

Query: 306 LLAAAVDTSATTISWALSELIKHPLSMKKIQDELEKVVGLDRMVEDSDLCNLKYLDMVIK 365
           + +   DT+A T+ WA++ELIKHP  M+K Q E+ +VVG    VE+ DL  L YL ++IK
Sbjct: 301 MFSGGTDTTAVTLEWAMAELIKHPDVMEKAQQEVRRVVGKKAKVEEEDLHQLHYLKLIIK 360

Query: 366 EIFRMHPPAPLLVPHESLDDCTVDNFYIPKKSRIIVNAWAIGRDPNVWIDAEKFFPERFI 425
           E  R+HP APLLVP ES  D  +  ++IP K+R+ +NAWAIGRDP  W +AE+F PERF+
Sbjct: 361 ETLRLHPVAPLLVPRESTRDVVIRGYHIPAKTRVFINAWAIGRDPKSWENAEEFLPERFV 420

Query: 426 GSQVDIKGRDFQLIPFGSGRRGCPGMQMGLIVVRLVLAQLVHCFNWELPNGTLPTKLNMT 480
            + VD KG+DFQLIPFG+GRRGCPG+  G+  V + LA L++ FNWELP       L+M+
Sbjct: 421 NNSVDFKGQDFQLIPFGAGRRGCPGIAFGISSVEISLANLLYWFNWELPGDLTKEDLDMS 480

BLAST of MC01g_new0383 vs. ExPASy Swiss-Prot
Match: A0A068Q721 (Cytochrome P450 71AP13 OS=Prunus mume OX=102107 GN=CYP71AP13 PE=2 SV=1)

HSP 1 Score: 405.2 bits (1040), Expect = 1.1e-111
Identity = 202/468 (43.16%), Postives = 292/468 (62.39%), Query Frame = 0

Query: 25  RSHARLPPGPKGFPIFGSLHLLGKLPHRALHRLSQKYGPIMHLRLGLVSTIVVSSPAAAE 84
           +   +LPP P   P+ G+LH LG  PH +L  L++KYGPI++L+LG + T+VVSS   A+
Sbjct: 43  KRRVKLPPSPPKLPVIGNLHQLGNKPHLSLRCLAEKYGPIIYLQLGEIPTVVVSSARLAK 102

Query: 85  LFLKTHDHVFASRPLIQASNHISYGRKGLGFAEYGPYWRNIRKMCTVELLSNLKVSSFRS 144
             LKTHD   +SRP I ++ H+ Y    + F+ YG YWR+IRK+C +ELLS  +V SF  
Sbjct: 103 EVLKTHDLALSSRPQIFSAKHLFYNCTDVVFSPYGAYWRHIRKICILELLSAKRVQSFSH 162

Query: 145 MRSEEVGLLIDHLREAAKSDVVVNLSSKVTSLVADMTCLMVFGKKYGD-EQLDERGFKAV 204
           +R EEV  L+  + E        NL+  +     D+ C + FG+ + +    D  GF+ +
Sbjct: 163 VREEEVARLVRRVAEFYPG--TTNLTKMLGLYANDVLCRVAFGRGFSEGGDYDRHGFQKM 222

Query: 205 IQEVMQLAAAPNLGDFIPYVAGL-DFQGFNRRAKSISKVIDGFLERIIDEHLQPKIGNKT 264
           ++E  +L    ++GDF P +  +    G   R +   +  D   ++++ +HL PK   + 
Sbjct: 223 LEEYQELLGGFSIGDFFPSMEFIHSLTGMKSRLQETFRRFDELFDQMVTDHLSPKREKEE 282

Query: 265 -KDFVDVMLDLMNSQAREYQIERSSIKAIILDLLAAAVDTSATTISWALSELIKHPLSMK 324
            KD VDV+LD+   ++ E  +   ++KAIILD+ AA  DT+  T+ W ++EL+ +   ++
Sbjct: 283 HKDLVDVLLDIQKKESTEMPLTMDNVKAIILDMFAAGTDTTFITLDWGMTELLMNRKVLE 342

Query: 325 KIQDELEKVVGLDRMVEDSDLCNLKYLDMVIKEIFRMHPPAPLLVPHESLDDCTVDNFYI 384
           + Q E+  VVG  R+V +SDL  L Y+  VIKEIFR+HPPAP+LVP ES++D T+D + I
Sbjct: 343 RAQAEVRGVVGERRVVLESDLPQLDYMKAVIKEIFRLHPPAPVLVPRESMEDVTIDGYDI 402

Query: 385 PKKSRIIVNAWAIGRDPNVWIDAEKFFPERFIGSQVDIKGRDFQLIPFGSGRRGCPGMQM 444
             K+RI VNAWAIGRDP  W D E F PERFIGS +D KG+DF+LIPFG+GRRGCP +  
Sbjct: 403 LAKTRIFVNAWAIGRDPESWEDPEAFEPERFIGSTIDFKGQDFELIPFGAGRRGCPAVTF 462

Query: 445 GLIVVRLVLAQLVHCFNWELPNGTLPTKLNMTEEFGLTCPRAEDLKVI 490
           G   + L LAQL+H F+WELP  T    L+MTE FG+T  R  +L V+
Sbjct: 463 GTATIELALAQLLHTFDWELPLDTAAKDLDMTEVFGITMHRIANLIVV 508

BLAST of MC01g_new0383 vs. ExPASy Swiss-Prot
Match: Q50EK4 (Cytochrome P450 750A1 OS=Pinus taeda OX=3352 GN=CYP750A1 PE=2 SV=1)

HSP 1 Score: 396.4 bits (1017), Expect = 4.9e-109
Identity = 206/505 (40.79%), Postives = 307/505 (60.79%), Query Frame = 0

Query: 8   ITILPLFLLFSSW-LWVLRSHARLPPGPKGFPIFGSLHLLGKLPHRALHRLSQKYGPIMH 67
           I I      FS W L   + + RLPPGP  +PI G+ H +    HR L  L++KYGPI+ 
Sbjct: 19  ILIATFIFFFSCWILHQSQRNERLPPGPYPWPIIGNFHQVRLPLHRTLKNLAEKYGPILF 78

Query: 68  LRLGLVSTIVVSSPAAAELFLKTHDHVFASRPLIQASNHISYGRKGLGFAEYGPYWRNIR 127
           LR G V T+VVSS   A+ FLKTHD +FASRP      +  Y  K + F+ YG +WR +R
Sbjct: 79  LRFGSVPTVVVSSSEKAKHFLKTHDLIFASRPPTSVGKYFFYNFKDIAFSPYGDHWRKMR 138

Query: 128 KMCTVELLSNLKVSSFRSMRSEEVGLLIDHLREAAKSD-VVVNLSSKVTSLVADMTCLMV 187
           K+C +ELL++ ++ SF+ +R EE+  +I  + E ++S  + VN+S  +++ +A++   ++
Sbjct: 139 KICVLELLTSKRIESFKHVRQEELSAMIHSIWEESESGRIAVNVSKAISTSLANILWRIL 198

Query: 188 FGKKYGDEQL--DERGFKAVIQEVMQLAAAPNLGDFIPYVAGLDFQGFNRRAKSISKVID 247
             KK+ D  L  D +GF  ++ EV     + N+GDFIPY+  LD QG  R  K  +   D
Sbjct: 199 ARKKFSDNDLGADGKGFADLVVEVSIAVGSLNIGDFIPYLDCLDLQGIKRALKKANARFD 258

Query: 248 GFLERIIDEHL--------QPKIGNKTKDFVDVMLDLMNSQAREYQIERSSIKAIILDLL 307
            F E++IDEH+        +   G   KD +DV+L++  +     ++ R  IKAI  +L 
Sbjct: 259 AFAEKMIDEHINASTIRNGEADAGCHVKDIIDVLLEMAKNDNTGAKVTREIIKAITYELF 318

Query: 308 AAAVDTSATTISWALSELIKHPLSMKKIQDELEKVVGLDRMVEDSDLCNLKYLDMVIKEI 367
           +A ++TSA  + WA+SEL++HP +MKK+Q E+E VVG    V++SDL ++ YL  V+KE 
Sbjct: 319 SAGMETSANVLEWAMSELLRHPHAMKKLQQEIESVVGQQGTVKESDLASIVYLHCVVKET 378

Query: 368 FRMHPPAPLLVPHESLDDCTVDNFYIPKKSRIIVNAWAIGRDPNVW-IDAEKFFPERFIG 427
            R++P  PL +PHESL+  TV  +YIPKK+ +I+N WAIGRDP+VW  DA +F PERF+ 
Sbjct: 379 LRLYPSLPLALPHESLEAVTVGGYYIPKKTMVIMNLWAIGRDPSVWGADASEFKPERFMQ 438

Query: 428 SQ---VDIKG--RDFQLIPFGSGRRGCPGMQMGLIVVRLVLAQLVHCFNWELPNGTLPTK 487
            +   +D+ G   DF+++PFG+GRR CPG  M ++ V   LAQL+H F+W +     P++
Sbjct: 439 MEENGIDLSGGQSDFRMLPFGAGRRTCPGSAMAILTVEFTLAQLLHTFDWRVEGD--PSE 498

Query: 488 LNMTEEFGLTCPRAEDLKVIPIYRI 495
           L+M E      PR   L   P  R+
Sbjct: 499 LDMKEACATKMPRQTPLLAYPRLRL 521

BLAST of MC01g_new0383 vs. NCBI nr
Match: XP_022132564.1 (cytochrome P450 CYP736A12-like [Momordica charantia])

HSP 1 Score: 991 bits (2561), Expect = 0.0
Identity = 494/495 (99.80%), Postives = 494/495 (99.80%), Query Frame = 0

Query: 1   MASVWLIITILPLFLLFSSWLWVLRSHARLPPGPKGFPIFGSLHLLGKLPHRALHRLSQK 60
           MASVWLIITILPLFLLFSSWLWVLRSHARLPPGPKGFPIFGSLHLLGKLPHRALHRLSQK
Sbjct: 1   MASVWLIITILPLFLLFSSWLWVLRSHARLPPGPKGFPIFGSLHLLGKLPHRALHRLSQK 60

Query: 61  YGPIMHLRLGLVSTIVVSSPAAAELFLKTHDHVFASRPLIQASNHISYGRKGLGFAEYGP 120
           YGPIMHLRLGLVSTIVVSSPAAAELFLKTHDHVFASRPLIQASNHISYGRKGLGFAEYGP
Sbjct: 61  YGPIMHLRLGLVSTIVVSSPAAAELFLKTHDHVFASRPLIQASNHISYGRKGLGFAEYGP 120

Query: 121 YWRNIRKMCTVELLSNLKVSSFRSMRSEEVGLLIDHLREAAKSDVVVNLSSKVTSLVADM 180
           YWRNIRKMCTVELLSNLKVSSFRSMRSEEVGLLIDHLREAAKSDVVVNLSSKVTSLVADM
Sbjct: 121 YWRNIRKMCTVELLSNLKVSSFRSMRSEEVGLLIDHLREAAKSDVVVNLSSKVTSLVADM 180

Query: 181 TCLMVFGKKYGDEQLDERGFKAVIQEVMQLAAAPNLGDFIPYVAGLDFQGFNRRAKSISK 240
           TCLMVFGKKYGDEQLDERGFKAVIQEVMQLAAAPNLGDFIPYVAGLDFQGFNRRAKSISK
Sbjct: 181 TCLMVFGKKYGDEQLDERGFKAVIQEVMQLAAAPNLGDFIPYVAGLDFQGFNRRAKSISK 240

Query: 241 VIDGFLERIIDEHLQPKIGNKTKDFVDVMLDLMNSQAREYQIERSSIKAIILDLLAAAVD 300
           VIDGFLERIIDEHLQPKIGNKTKDFVDVMLDLMNSQA EYQIERSSIKAIILDLLAAAVD
Sbjct: 241 VIDGFLERIIDEHLQPKIGNKTKDFVDVMLDLMNSQATEYQIERSSIKAIILDLLAAAVD 300

Query: 301 TSATTISWALSELIKHPLSMKKIQDELEKVVGLDRMVEDSDLCNLKYLDMVIKEIFRMHP 360
           TSATTISWALSELIKHPLSMKKIQDELEKVVGLDRMVEDSDLCNLKYLDMVIKEIFRMHP
Sbjct: 301 TSATTISWALSELIKHPLSMKKIQDELEKVVGLDRMVEDSDLCNLKYLDMVIKEIFRMHP 360

Query: 361 PAPLLVPHESLDDCTVDNFYIPKKSRIIVNAWAIGRDPNVWIDAEKFFPERFIGSQVDIK 420
           PAPLLVPHESLDDCTVDNFYIPKKSRIIVNAWAIGRDPNVWIDAEKFFPERFIGSQVDIK
Sbjct: 361 PAPLLVPHESLDDCTVDNFYIPKKSRIIVNAWAIGRDPNVWIDAEKFFPERFIGSQVDIK 420

Query: 421 GRDFQLIPFGSGRRGCPGMQMGLIVVRLVLAQLVHCFNWELPNGTLPTKLNMTEEFGLTC 480
           GRDFQLIPFGSGRRGCPGMQMGLIVVRLVLAQLVHCFNWELPNGTLPTKLNMTEEFGLTC
Sbjct: 421 GRDFQLIPFGSGRRGCPGMQMGLIVVRLVLAQLVHCFNWELPNGTLPTKLNMTEEFGLTC 480

Query: 481 PRAEDLKVIPIYRIL 495
           PRAEDLKVIPIYRIL
Sbjct: 481 PRAEDLKVIPIYRIL 495

BLAST of MC01g_new0383 vs. NCBI nr
Match: XP_008440675.1 (PREDICTED: cytochrome P450 CYP736A12-like [Cucumis melo] >KAA0036257.1 cytochrome P450 CYP736A12-like protein [Cucumis melo var. makuwa] >TYK12651.1 cytochrome P450 CYP736A12-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 767 bits (1980), Expect = 7.67e-276
Identity = 369/494 (74.70%), Postives = 432/494 (87.45%), Query Frame = 0

Query: 1   MASVWLIITILPLFLLFSSWLWVLRSHARLPPGPKGFPIFGSLHLLGKLPHRALHRLSQK 60
           MA ++L + ++ L +L  + L+  RS  +LPPGP+GFPIFG LHLLGKLPHR L  LS+K
Sbjct: 1   MAWIFLAVALVGLAILLRTRLY--RSPNKLPPGPRGFPIFGCLHLLGKLPHRDLRSLSKK 60

Query: 61  YGPIMHLRLGLVSTIVVSSPAAAELFLKTHDHVFASRPLIQASNHISYGRKGLGFAEYGP 120
           YG IM++RLGLV T++VSSP AAELFLKTHD VFASRP +QAS ++SYG+K LGFA+YGP
Sbjct: 61  YGSIMYMRLGLVPTVIVSSPQAAELFLKTHDTVFASRPFVQASKYMSYGQKNLGFAQYGP 120

Query: 121 YWRNIRKMCTVELLSNLKVSSFRSMRSEEVGLLIDHLREAAKSDVVVNLSSKVTSLVADM 180
           YWRN+RKMCT+ELLS++KV SFRSMR EE+GLL+D+LR+AAK  V+VNLSSK+ SL  DM
Sbjct: 121 YWRNMRKMCTLELLSSVKVKSFRSMRMEELGLLVDYLRDAAKKRVIVNLSSKICSLNTDM 180

Query: 181 TCLMVFGKKYGDEQLDERGFKAVIQEVMQLAAAPNLGDFIPYVAGLDFQGFNRRAKSISK 240
           TCLMVFGKKY D++ DERGFK+VIQE MQL A+PNLGDFIP +AGLD QG +RR+K+ISK
Sbjct: 181 TCLMVFGKKYEDQEFDERGFKSVIQEAMQLVASPNLGDFIPQIAGLDLQGLDRRSKAISK 240

Query: 241 VIDGFLERIIDEHLQPKIGNKTKDFVDVMLDLMNSQAREYQIERSSIKAIILDLLAAAVD 300
           + DGFLERIIDEHL+ K  NKT DFVDVML++M SQ  EYQIERS+IKA+ILD+L AA+D
Sbjct: 241 IFDGFLERIIDEHLESKNENKTMDFVDVMLEIMGSQETEYQIERSNIKALILDMLVAAMD 300

Query: 301 TSATTISWALSELIKHPLSMKKIQDELEKVVGLDRMVEDSDLCNLKYLDMVIKEIFRMHP 360
           TSATTI WA+ ELIKHP  MKK+QDELEKVVGLDR VE++DL +L+YLDMV+KEI R+HP
Sbjct: 301 TSATTIGWAIPELIKHPHVMKKMQDELEKVVGLDRKVEETDLDHLEYLDMVVKEILRLHP 360

Query: 361 PAPLLVPHESLDDCTVDNFYIPKKSRIIVNAWAIGRDPNVWIDAEKFFPERFIGSQVDIK 420
           PAPLLVPHE+L+DC VD FYIPKKSRIIVN WAIGRDPN WID EKFFPERFIGSQVD++
Sbjct: 361 PAPLLVPHEALEDCIVDGFYIPKKSRIIVNGWAIGRDPNFWIDPEKFFPERFIGSQVDVR 420

Query: 421 GRDFQLIPFGSGRRGCPGMQMGLIVVRLVLAQLVHCFNWELPNGTLPTKLNMTEEFGLTC 480
           GRDFQLIPFGSGRRGCPGMQMGL VVRLV+AQLVHCF+WELPNGTLP KL+MTEEFGLTC
Sbjct: 421 GRDFQLIPFGSGRRGCPGMQMGLTVVRLVIAQLVHCFDWELPNGTLPVKLDMTEEFGLTC 480

Query: 481 PRAEDLKVIPIYRI 494
           PRA+DL V PIYR+
Sbjct: 481 PRAQDLMVTPIYRL 492

BLAST of MC01g_new0383 vs. NCBI nr
Match: XP_031743720.1 (cytochrome P450 CYP736A12-like [Cucumis sativus] >KAE8647578.1 hypothetical protein Csa_004254 [Cucumis sativus])

HSP 1 Score: 753 bits (1943), Expect = 3.28e-270
Identity = 360/494 (72.87%), Postives = 428/494 (86.64%), Query Frame = 0

Query: 1   MASVWLIITILPLFLLFSSWLWVLRSHARLPPGPKGFPIFGSLHLLGKLPHRALHRLSQK 60
           MA ++L + ++ L LL  + L+  RS  +LPPGP+GFP+FG LHLLGKLPHR L  LS+K
Sbjct: 1   MAWIFLAVALVGLALLLRTRLY--RSPNKLPPGPRGFPVFGCLHLLGKLPHRDLRSLSKK 60

Query: 61  YGPIMHLRLGLVSTIVVSSPAAAELFLKTHDHVFASRPLIQASNHISYGRKGLGFAEYGP 120
           YG IM++RLGLV TI+VSSP AAELFLKTHD VFASRP +QAS ++SYG+K LGFA+YGP
Sbjct: 61  YGSIMYMRLGLVPTIIVSSPQAAELFLKTHDSVFASRPFVQASKYMSYGQKNLGFAQYGP 120

Query: 121 YWRNIRKMCTVELLSNLKVSSFRSMRSEEVGLLIDHLREAAKSDVVVNLSSKVTSLVADM 180
           YWRN+RKMC +ELLS++KV SFRSMR EE+GL +D+LR+AAK  V+VNLSSK+ SL  DM
Sbjct: 121 YWRNMRKMCRLELLSSVKVESFRSMRMEELGLFVDYLRDAAKKRVIVNLSSKICSLNTDM 180

Query: 181 TCLMVFGKKYGDEQLDERGFKAVIQEVMQLAAAPNLGDFIPYVAGLDFQGFNRRAKSISK 240
           TCLMVFGKKY D++ DERGFK+VIQE MQ+ A+PNLGDFIP +A LD QG +RR+K++SK
Sbjct: 181 TCLMVFGKKYKDQEFDERGFKSVIQEAMQIVASPNLGDFIPQIAVLDLQGLDRRSKAVSK 240

Query: 241 VIDGFLERIIDEHLQPKIGNKTKDFVDVMLDLMNSQAREYQIERSSIKAIILDLLAAAVD 300
           + D F ERIIDEHL+ +  NKTKDFVDVML++M+S   EYQIERS+IKAIILD+L AA+D
Sbjct: 241 IFDEFFERIIDEHLESRYENKTKDFVDVMLEIMDSHGTEYQIERSNIKAIILDMLVAAMD 300

Query: 301 TSATTISWALSELIKHPLSMKKIQDELEKVVGLDRMVEDSDLCNLKYLDMVIKEIFRMHP 360
           TSATTI WA+ ELIKHP  MKK+QDEL+KVVGLDR VE++DL +L+YLDMV+KEI R+HP
Sbjct: 301 TSATTIGWAIPELIKHPHVMKKMQDELQKVVGLDRKVEETDLDHLQYLDMVVKEILRLHP 360

Query: 361 PAPLLVPHESLDDCTVDNFYIPKKSRIIVNAWAIGRDPNVWIDAEKFFPERFIGSQVDIK 420
           PAPLLVPHE+L+DC VD FYIPKKSRIIVN WAIGRDPN WID EKFFPERFIGSQVD++
Sbjct: 361 PAPLLVPHEALEDCIVDGFYIPKKSRIIVNGWAIGRDPNFWIDPEKFFPERFIGSQVDVR 420

Query: 421 GRDFQLIPFGSGRRGCPGMQMGLIVVRLVLAQLVHCFNWELPNGTLPTKLNMTEEFGLTC 480
           G+DFQLIPFGSGRRGCPGMQMGL VVRLV+AQLVHCF+WELPNGTLP +L+MTEEFGLTC
Sbjct: 421 GKDFQLIPFGSGRRGCPGMQMGLTVVRLVIAQLVHCFDWELPNGTLPVELDMTEEFGLTC 480

Query: 481 PRAEDLKVIPIYRI 494
           PRA+DL V PIYR+
Sbjct: 481 PRAQDLMVTPIYRL 492

BLAST of MC01g_new0383 vs. NCBI nr
Match: XP_011658487.2 (cytochrome P450 CYP736A12 [Cucumis sativus])

HSP 1 Score: 749 bits (1935), Expect = 5.41e-269
Identity = 351/470 (74.68%), Postives = 415/470 (88.30%), Query Frame = 0

Query: 25  RSHARLPPGPKGFPIFGSLHLLGKLPHRALHRLSQKYGPIMHLRLGLVSTIVVSSPAAAE 84
           RS  +LPPGP+GFP+FG LHLLGKLPHR L  LS+KYG IM++RLGLV TI+VSSP AAE
Sbjct: 23  RSPNKLPPGPRGFPVFGCLHLLGKLPHRDLQSLSEKYGSIMYMRLGLVPTIIVSSPHAAE 82

Query: 85  LFLKTHDHVFASRPLIQASNHISYGRKGLGFAEYGPYWRNIRKMCTVELLSNLKVSSFRS 144
           LFLKTHD VFASRP +QAS +++YG+K L F++YGPYWRN+RKMCT+ELLS++KV SFRS
Sbjct: 83  LFLKTHDTVFASRPFVQASKYMAYGQKNLAFSQYGPYWRNLRKMCTLELLSSVKVKSFRS 142

Query: 145 MRSEEVGLLIDHLREAAKSDVVVNLSSKVTSLVADMTCLMVFGKKYGDEQLDERGFKAVI 204
           MR EE+GL +D+LR+AA+  V+VNLSSK+ SL  DMTCLMVFGKKY D++ DERGFK+VI
Sbjct: 143 MRMEELGLFVDYLRDAARKRVIVNLSSKICSLNTDMTCLMVFGKKYKDQEFDERGFKSVI 202

Query: 205 QEVMQLAAAPNLGDFIPYVAGLDFQGFNRRAKSISKVIDGFLERIIDEHLQPKIGNKTKD 264
           QE MQ+ A+PNLGDFIP +A LD QG +RR+K++SK+ D F ERIIDEHL+ +  NKTKD
Sbjct: 203 QEAMQIVASPNLGDFIPQIAVLDLQGLDRRSKAVSKIFDEFFERIIDEHLESRYENKTKD 262

Query: 265 FVDVMLDLMNSQAREYQIERSSIKAIILDLLAAAVDTSATTISWALSELIKHPLSMKKIQ 324
           FVDVML++M+SQ  EYQIERS+IKAIILD+L AA+DTSATTI WA+ ELIKHP  MKK+Q
Sbjct: 263 FVDVMLEIMDSQGTEYQIERSNIKAIILDMLVAAMDTSATTIGWAIPELIKHPHVMKKMQ 322

Query: 325 DELEKVVGLDRMVEDSDLCNLKYLDMVIKEIFRMHPPAPLLVPHESLDDCTVDNFYIPKK 384
           DEL+KVVGLDR VE++DL +L+YLDMV+KEI R+HPPAPLLVPHE+L+DC VD FYIPKK
Sbjct: 323 DELQKVVGLDRKVEETDLDHLQYLDMVVKEILRLHPPAPLLVPHEALEDCIVDGFYIPKK 382

Query: 385 SRIIVNAWAIGRDPNVWIDAEKFFPERFIGSQVDIKGRDFQLIPFGSGRRGCPGMQMGLI 444
           SRIIVN WAIGRDPN WID EKFFPERFIGSQVD++G+DFQLIPFGSGRRGCPGMQMGL 
Sbjct: 383 SRIIVNGWAIGRDPNFWIDPEKFFPERFIGSQVDVRGKDFQLIPFGSGRRGCPGMQMGLT 442

Query: 445 VVRLVLAQLVHCFNWELPNGTLPTKLNMTEEFGLTCPRAEDLKVIPIYRI 494
           VVRLV+AQLVHCF+WELPNGTLP +L+MTEEFGLTCPRA+DL V PIYR+
Sbjct: 443 VVRLVIAQLVHCFDWELPNGTLPVELDMTEEFGLTCPRAQDLMVTPIYRL 492

BLAST of MC01g_new0383 vs. NCBI nr
Match: XP_022133318.1 (cytochrome P450 CYP736A12-like [Momordica charantia])

HSP 1 Score: 748 bits (1932), Expect = 1.61e-268
Identity = 352/473 (74.42%), Postives = 413/473 (87.32%), Query Frame = 0

Query: 22  WVLRSHARLPPGPKGFPIFGSLHLLGKLPHRALHRLSQKYGPIMHLRLGLVSTIVVSSPA 81
           W+ RS  +LPPGP+GFP+FGSLHLLGKLPHR L  LSQKYG IM++RLG+V TI+V+SP 
Sbjct: 21  WLFRSQNKLPPGPRGFPVFGSLHLLGKLPHRDLRDLSQKYGSIMYMRLGIVPTIIVTSPQ 80

Query: 82  AAELFLKTHDHVFASRPLIQASNHISYGRKGLGFAEYGPYWRNIRKMCTVELLSNLKVSS 141
           AAELFLKTHD VFASRP  +AS ++SYG+K LGFA+YG YWRN+RKMCT+ELLS+ KV+S
Sbjct: 81  AAELFLKTHDLVFASRPFFEASKYMSYGQKNLGFAQYGSYWRNMRKMCTLELLSHHKVNS 140

Query: 142 FRSMRSEEVGLLIDHLREAAKSDVVVNLSSKVTSLVADMTCLMVFGKKYGDEQLDERGFK 201
           FRSMR EEVGLL+ +LR+AAK  VV+NLSSK+ SL  DMTCLMVFGKKY D + DERGFK
Sbjct: 141 FRSMRREEVGLLVHYLRDAAKDHVVINLSSKIASLNTDMTCLMVFGKKYADAEFDERGFK 200

Query: 202 AVIQEVMQLAAAPNLGDFIPYVAGLDFQGFNRRAKSISKVIDGFLERIIDEHLQPKIGNK 261
           +VIQE MQL A PNLGDFIP +A LD QG NRR+K+ISK+ DGF E+IIDEHL+ K  NK
Sbjct: 201 SVIQEAMQLVATPNLGDFIPCIAALDLQGLNRRSKAISKIFDGFFEKIIDEHLESKNENK 260

Query: 262 TKDFVDVMLDLMNSQAREYQIERSSIKAIILDLLAAAVDTSATTISWALSELIKHPLSMK 321
           TKDFVDVMLD+M SQ  EYQI+RSSIKAIILD+L AA+DTSATT+ WA++ELIKHP  MK
Sbjct: 261 TKDFVDVMLDIMGSQETEYQIDRSSIKAIILDMLVAAMDTSATTVGWAIAELIKHPQVMK 320

Query: 322 KIQDELEKVVGLDRMVEDSDLCNLKYLDMVIKEIFRMHPPAPLLVPHESLDDCTVDNFYI 381
           K+Q+EL+KVVGLDR VE+SDL +L+YLDM +KEI R+HP APLLVPHESL+DCTV+ ++I
Sbjct: 321 KLQEELQKVVGLDRRVEESDLDHLEYLDMFVKEILRVHPAAPLLVPHESLEDCTVNGYHI 380

Query: 382 PKKSRIIVNAWAIGRDPNVWIDAEKFFPERFIGSQVDIKGRDFQLIPFGSGRRGCPGMQM 441
           PKKSRIIVNAWAIG+DPN W+D EKFFPERFIGSQVD+KGRDFQLIPFGSGRRGCPGM M
Sbjct: 381 PKKSRIIVNAWAIGQDPNSWVDPEKFFPERFIGSQVDVKGRDFQLIPFGSGRRGCPGMHM 440

Query: 442 GLIVVRLVLAQLVHCFNWELPNGTLPTKLNMTEEFGLTCPRAEDLKVIPIYRI 494
           GL+VV  V+AQLVHCF+W+LPNG LP +L+MTEEFGLTCPRA+DL V PIYR+
Sbjct: 441 GLVVVHFVIAQLVHCFDWKLPNGMLPAELDMTEEFGLTCPRAQDLMVTPIYRL 493

BLAST of MC01g_new0383 vs. ExPASy TrEMBL
Match: A0A6J1BTE3 (cytochrome P450 CYP736A12-like OS=Momordica charantia OX=3673 GN=LOC111005393 PE=3 SV=1)

HSP 1 Score: 991 bits (2561), Expect = 0.0
Identity = 494/495 (99.80%), Postives = 494/495 (99.80%), Query Frame = 0

Query: 1   MASVWLIITILPLFLLFSSWLWVLRSHARLPPGPKGFPIFGSLHLLGKLPHRALHRLSQK 60
           MASVWLIITILPLFLLFSSWLWVLRSHARLPPGPKGFPIFGSLHLLGKLPHRALHRLSQK
Sbjct: 1   MASVWLIITILPLFLLFSSWLWVLRSHARLPPGPKGFPIFGSLHLLGKLPHRALHRLSQK 60

Query: 61  YGPIMHLRLGLVSTIVVSSPAAAELFLKTHDHVFASRPLIQASNHISYGRKGLGFAEYGP 120
           YGPIMHLRLGLVSTIVVSSPAAAELFLKTHDHVFASRPLIQASNHISYGRKGLGFAEYGP
Sbjct: 61  YGPIMHLRLGLVSTIVVSSPAAAELFLKTHDHVFASRPLIQASNHISYGRKGLGFAEYGP 120

Query: 121 YWRNIRKMCTVELLSNLKVSSFRSMRSEEVGLLIDHLREAAKSDVVVNLSSKVTSLVADM 180
           YWRNIRKMCTVELLSNLKVSSFRSMRSEEVGLLIDHLREAAKSDVVVNLSSKVTSLVADM
Sbjct: 121 YWRNIRKMCTVELLSNLKVSSFRSMRSEEVGLLIDHLREAAKSDVVVNLSSKVTSLVADM 180

Query: 181 TCLMVFGKKYGDEQLDERGFKAVIQEVMQLAAAPNLGDFIPYVAGLDFQGFNRRAKSISK 240
           TCLMVFGKKYGDEQLDERGFKAVIQEVMQLAAAPNLGDFIPYVAGLDFQGFNRRAKSISK
Sbjct: 181 TCLMVFGKKYGDEQLDERGFKAVIQEVMQLAAAPNLGDFIPYVAGLDFQGFNRRAKSISK 240

Query: 241 VIDGFLERIIDEHLQPKIGNKTKDFVDVMLDLMNSQAREYQIERSSIKAIILDLLAAAVD 300
           VIDGFLERIIDEHLQPKIGNKTKDFVDVMLDLMNSQA EYQIERSSIKAIILDLLAAAVD
Sbjct: 241 VIDGFLERIIDEHLQPKIGNKTKDFVDVMLDLMNSQATEYQIERSSIKAIILDLLAAAVD 300

Query: 301 TSATTISWALSELIKHPLSMKKIQDELEKVVGLDRMVEDSDLCNLKYLDMVIKEIFRMHP 360
           TSATTISWALSELIKHPLSMKKIQDELEKVVGLDRMVEDSDLCNLKYLDMVIKEIFRMHP
Sbjct: 301 TSATTISWALSELIKHPLSMKKIQDELEKVVGLDRMVEDSDLCNLKYLDMVIKEIFRMHP 360

Query: 361 PAPLLVPHESLDDCTVDNFYIPKKSRIIVNAWAIGRDPNVWIDAEKFFPERFIGSQVDIK 420
           PAPLLVPHESLDDCTVDNFYIPKKSRIIVNAWAIGRDPNVWIDAEKFFPERFIGSQVDIK
Sbjct: 361 PAPLLVPHESLDDCTVDNFYIPKKSRIIVNAWAIGRDPNVWIDAEKFFPERFIGSQVDIK 420

Query: 421 GRDFQLIPFGSGRRGCPGMQMGLIVVRLVLAQLVHCFNWELPNGTLPTKLNMTEEFGLTC 480
           GRDFQLIPFGSGRRGCPGMQMGLIVVRLVLAQLVHCFNWELPNGTLPTKLNMTEEFGLTC
Sbjct: 421 GRDFQLIPFGSGRRGCPGMQMGLIVVRLVLAQLVHCFNWELPNGTLPTKLNMTEEFGLTC 480

Query: 481 PRAEDLKVIPIYRIL 495
           PRAEDLKVIPIYRIL
Sbjct: 481 PRAEDLKVIPIYRIL 495

BLAST of MC01g_new0383 vs. ExPASy TrEMBL
Match: A0A5A7T3U2 (Cytochrome P450 CYP736A12-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G002390 PE=3 SV=1)

HSP 1 Score: 767 bits (1980), Expect = 3.71e-276
Identity = 369/494 (74.70%), Postives = 432/494 (87.45%), Query Frame = 0

Query: 1   MASVWLIITILPLFLLFSSWLWVLRSHARLPPGPKGFPIFGSLHLLGKLPHRALHRLSQK 60
           MA ++L + ++ L +L  + L+  RS  +LPPGP+GFPIFG LHLLGKLPHR L  LS+K
Sbjct: 1   MAWIFLAVALVGLAILLRTRLY--RSPNKLPPGPRGFPIFGCLHLLGKLPHRDLRSLSKK 60

Query: 61  YGPIMHLRLGLVSTIVVSSPAAAELFLKTHDHVFASRPLIQASNHISYGRKGLGFAEYGP 120
           YG IM++RLGLV T++VSSP AAELFLKTHD VFASRP +QAS ++SYG+K LGFA+YGP
Sbjct: 61  YGSIMYMRLGLVPTVIVSSPQAAELFLKTHDTVFASRPFVQASKYMSYGQKNLGFAQYGP 120

Query: 121 YWRNIRKMCTVELLSNLKVSSFRSMRSEEVGLLIDHLREAAKSDVVVNLSSKVTSLVADM 180
           YWRN+RKMCT+ELLS++KV SFRSMR EE+GLL+D+LR+AAK  V+VNLSSK+ SL  DM
Sbjct: 121 YWRNMRKMCTLELLSSVKVKSFRSMRMEELGLLVDYLRDAAKKRVIVNLSSKICSLNTDM 180

Query: 181 TCLMVFGKKYGDEQLDERGFKAVIQEVMQLAAAPNLGDFIPYVAGLDFQGFNRRAKSISK 240
           TCLMVFGKKY D++ DERGFK+VIQE MQL A+PNLGDFIP +AGLD QG +RR+K+ISK
Sbjct: 181 TCLMVFGKKYEDQEFDERGFKSVIQEAMQLVASPNLGDFIPQIAGLDLQGLDRRSKAISK 240

Query: 241 VIDGFLERIIDEHLQPKIGNKTKDFVDVMLDLMNSQAREYQIERSSIKAIILDLLAAAVD 300
           + DGFLERIIDEHL+ K  NKT DFVDVML++M SQ  EYQIERS+IKA+ILD+L AA+D
Sbjct: 241 IFDGFLERIIDEHLESKNENKTMDFVDVMLEIMGSQETEYQIERSNIKALILDMLVAAMD 300

Query: 301 TSATTISWALSELIKHPLSMKKIQDELEKVVGLDRMVEDSDLCNLKYLDMVIKEIFRMHP 360
           TSATTI WA+ ELIKHP  MKK+QDELEKVVGLDR VE++DL +L+YLDMV+KEI R+HP
Sbjct: 301 TSATTIGWAIPELIKHPHVMKKMQDELEKVVGLDRKVEETDLDHLEYLDMVVKEILRLHP 360

Query: 361 PAPLLVPHESLDDCTVDNFYIPKKSRIIVNAWAIGRDPNVWIDAEKFFPERFIGSQVDIK 420
           PAPLLVPHE+L+DC VD FYIPKKSRIIVN WAIGRDPN WID EKFFPERFIGSQVD++
Sbjct: 361 PAPLLVPHEALEDCIVDGFYIPKKSRIIVNGWAIGRDPNFWIDPEKFFPERFIGSQVDVR 420

Query: 421 GRDFQLIPFGSGRRGCPGMQMGLIVVRLVLAQLVHCFNWELPNGTLPTKLNMTEEFGLTC 480
           GRDFQLIPFGSGRRGCPGMQMGL VVRLV+AQLVHCF+WELPNGTLP KL+MTEEFGLTC
Sbjct: 421 GRDFQLIPFGSGRRGCPGMQMGLTVVRLVIAQLVHCFDWELPNGTLPVKLDMTEEFGLTC 480

Query: 481 PRAEDLKVIPIYRI 494
           PRA+DL V PIYR+
Sbjct: 481 PRAQDLMVTPIYRL 492

BLAST of MC01g_new0383 vs. ExPASy TrEMBL
Match: A0A1S3B1P7 (cytochrome P450 CYP736A12-like OS=Cucumis melo OX=3656 GN=LOC103485015 PE=3 SV=1)

HSP 1 Score: 767 bits (1980), Expect = 3.71e-276
Identity = 369/494 (74.70%), Postives = 432/494 (87.45%), Query Frame = 0

Query: 1   MASVWLIITILPLFLLFSSWLWVLRSHARLPPGPKGFPIFGSLHLLGKLPHRALHRLSQK 60
           MA ++L + ++ L +L  + L+  RS  +LPPGP+GFPIFG LHLLGKLPHR L  LS+K
Sbjct: 1   MAWIFLAVALVGLAILLRTRLY--RSPNKLPPGPRGFPIFGCLHLLGKLPHRDLRSLSKK 60

Query: 61  YGPIMHLRLGLVSTIVVSSPAAAELFLKTHDHVFASRPLIQASNHISYGRKGLGFAEYGP 120
           YG IM++RLGLV T++VSSP AAELFLKTHD VFASRP +QAS ++SYG+K LGFA+YGP
Sbjct: 61  YGSIMYMRLGLVPTVIVSSPQAAELFLKTHDTVFASRPFVQASKYMSYGQKNLGFAQYGP 120

Query: 121 YWRNIRKMCTVELLSNLKVSSFRSMRSEEVGLLIDHLREAAKSDVVVNLSSKVTSLVADM 180
           YWRN+RKMCT+ELLS++KV SFRSMR EE+GLL+D+LR+AAK  V+VNLSSK+ SL  DM
Sbjct: 121 YWRNMRKMCTLELLSSVKVKSFRSMRMEELGLLVDYLRDAAKKRVIVNLSSKICSLNTDM 180

Query: 181 TCLMVFGKKYGDEQLDERGFKAVIQEVMQLAAAPNLGDFIPYVAGLDFQGFNRRAKSISK 240
           TCLMVFGKKY D++ DERGFK+VIQE MQL A+PNLGDFIP +AGLD QG +RR+K+ISK
Sbjct: 181 TCLMVFGKKYEDQEFDERGFKSVIQEAMQLVASPNLGDFIPQIAGLDLQGLDRRSKAISK 240

Query: 241 VIDGFLERIIDEHLQPKIGNKTKDFVDVMLDLMNSQAREYQIERSSIKAIILDLLAAAVD 300
           + DGFLERIIDEHL+ K  NKT DFVDVML++M SQ  EYQIERS+IKA+ILD+L AA+D
Sbjct: 241 IFDGFLERIIDEHLESKNENKTMDFVDVMLEIMGSQETEYQIERSNIKALILDMLVAAMD 300

Query: 301 TSATTISWALSELIKHPLSMKKIQDELEKVVGLDRMVEDSDLCNLKYLDMVIKEIFRMHP 360
           TSATTI WA+ ELIKHP  MKK+QDELEKVVGLDR VE++DL +L+YLDMV+KEI R+HP
Sbjct: 301 TSATTIGWAIPELIKHPHVMKKMQDELEKVVGLDRKVEETDLDHLEYLDMVVKEILRLHP 360

Query: 361 PAPLLVPHESLDDCTVDNFYIPKKSRIIVNAWAIGRDPNVWIDAEKFFPERFIGSQVDIK 420
           PAPLLVPHE+L+DC VD FYIPKKSRIIVN WAIGRDPN WID EKFFPERFIGSQVD++
Sbjct: 361 PAPLLVPHEALEDCIVDGFYIPKKSRIIVNGWAIGRDPNFWIDPEKFFPERFIGSQVDVR 420

Query: 421 GRDFQLIPFGSGRRGCPGMQMGLIVVRLVLAQLVHCFNWELPNGTLPTKLNMTEEFGLTC 480
           GRDFQLIPFGSGRRGCPGMQMGL VVRLV+AQLVHCF+WELPNGTLP KL+MTEEFGLTC
Sbjct: 421 GRDFQLIPFGSGRRGCPGMQMGLTVVRLVIAQLVHCFDWELPNGTLPVKLDMTEEFGLTC 480

Query: 481 PRAEDLKVIPIYRI 494
           PRA+DL V PIYR+
Sbjct: 481 PRAQDLMVTPIYRL 492

BLAST of MC01g_new0383 vs. ExPASy TrEMBL
Match: A0A6J1BYU0 (cytochrome P450 CYP736A12-like OS=Momordica charantia OX=3673 GN=LOC111005927 PE=3 SV=1)

HSP 1 Score: 748 bits (1932), Expect = 7.78e-269
Identity = 352/473 (74.42%), Postives = 413/473 (87.32%), Query Frame = 0

Query: 22  WVLRSHARLPPGPKGFPIFGSLHLLGKLPHRALHRLSQKYGPIMHLRLGLVSTIVVSSPA 81
           W+ RS  +LPPGP+GFP+FGSLHLLGKLPHR L  LSQKYG IM++RLG+V TI+V+SP 
Sbjct: 21  WLFRSQNKLPPGPRGFPVFGSLHLLGKLPHRDLRDLSQKYGSIMYMRLGIVPTIIVTSPQ 80

Query: 82  AAELFLKTHDHVFASRPLIQASNHISYGRKGLGFAEYGPYWRNIRKMCTVELLSNLKVSS 141
           AAELFLKTHD VFASRP  +AS ++SYG+K LGFA+YG YWRN+RKMCT+ELLS+ KV+S
Sbjct: 81  AAELFLKTHDLVFASRPFFEASKYMSYGQKNLGFAQYGSYWRNMRKMCTLELLSHHKVNS 140

Query: 142 FRSMRSEEVGLLIDHLREAAKSDVVVNLSSKVTSLVADMTCLMVFGKKYGDEQLDERGFK 201
           FRSMR EEVGLL+ +LR+AAK  VV+NLSSK+ SL  DMTCLMVFGKKY D + DERGFK
Sbjct: 141 FRSMRREEVGLLVHYLRDAAKDHVVINLSSKIASLNTDMTCLMVFGKKYADAEFDERGFK 200

Query: 202 AVIQEVMQLAAAPNLGDFIPYVAGLDFQGFNRRAKSISKVIDGFLERIIDEHLQPKIGNK 261
           +VIQE MQL A PNLGDFIP +A LD QG NRR+K+ISK+ DGF E+IIDEHL+ K  NK
Sbjct: 201 SVIQEAMQLVATPNLGDFIPCIAALDLQGLNRRSKAISKIFDGFFEKIIDEHLESKNENK 260

Query: 262 TKDFVDVMLDLMNSQAREYQIERSSIKAIILDLLAAAVDTSATTISWALSELIKHPLSMK 321
           TKDFVDVMLD+M SQ  EYQI+RSSIKAIILD+L AA+DTSATT+ WA++ELIKHP  MK
Sbjct: 261 TKDFVDVMLDIMGSQETEYQIDRSSIKAIILDMLVAAMDTSATTVGWAIAELIKHPQVMK 320

Query: 322 KIQDELEKVVGLDRMVEDSDLCNLKYLDMVIKEIFRMHPPAPLLVPHESLDDCTVDNFYI 381
           K+Q+EL+KVVGLDR VE+SDL +L+YLDM +KEI R+HP APLLVPHESL+DCTV+ ++I
Sbjct: 321 KLQEELQKVVGLDRRVEESDLDHLEYLDMFVKEILRVHPAAPLLVPHESLEDCTVNGYHI 380

Query: 382 PKKSRIIVNAWAIGRDPNVWIDAEKFFPERFIGSQVDIKGRDFQLIPFGSGRRGCPGMQM 441
           PKKSRIIVNAWAIG+DPN W+D EKFFPERFIGSQVD+KGRDFQLIPFGSGRRGCPGM M
Sbjct: 381 PKKSRIIVNAWAIGQDPNSWVDPEKFFPERFIGSQVDVKGRDFQLIPFGSGRRGCPGMHM 440

Query: 442 GLIVVRLVLAQLVHCFNWELPNGTLPTKLNMTEEFGLTCPRAEDLKVIPIYRI 494
           GL+VV  V+AQLVHCF+W+LPNG LP +L+MTEEFGLTCPRA+DL V PIYR+
Sbjct: 441 GLVVVHFVIAQLVHCFDWKLPNGMLPAELDMTEEFGLTCPRAQDLMVTPIYRL 493

BLAST of MC01g_new0383 vs. ExPASy TrEMBL
Match: A0A6J1GEV3 (cytochrome P450 CYP736A12-like OS=Cucurbita moschata OX=3662 GN=LOC111453532 PE=3 SV=1)

HSP 1 Score: 744 bits (1921), Expect = 3.67e-267
Identity = 355/480 (73.96%), Postives = 418/480 (87.08%), Query Frame = 0

Query: 15  LLFSSWLWVLRSHARLPPGPKGFPIFGSLHLLGKLPHRALHRLSQKYGPIMHLRLGLVST 74
           LLF+SWL   R   +LPPGP+G PI GSLHLLGKLPHR L  LSQKYG IM++RLGLV T
Sbjct: 16  LLFTSWLH--RGPNQLPPGPRGLPILGSLHLLGKLPHRDLRALSQKYGSIMYMRLGLVPT 75

Query: 75  IVVSSPAAAELFLKTHDHVFASRPLIQASNHISYGRKGLGFAEYGPYWRNIRKMCTVELL 134
           IVVSSP AAELFLK+HD VFASRP +QAS ++SYG+K L FA YG YWRN+RKMCT+ELL
Sbjct: 76  IVVSSPQAAELFLKSHDIVFASRPFVQASKYMSYGQKNLAFAPYGSYWRNMRKMCTLELL 135

Query: 135 SNLKVSSFRSMRSEEVGLLIDHLREAAKSDVVVNLSSKVTSLVADMTCLMVFGKKYGDEQ 194
           SN+KV+SFRSMR EE+GLL+D+LR+AA +  VVNLSSKV  LV DMTCLMVFGKKY D++
Sbjct: 136 SNVKVNSFRSMRREELGLLVDYLRDAAMNRDVVNLSSKVCCLVTDMTCLMVFGKKYADQE 195

Query: 195 LDERGFKAVIQEVMQLAAAPNLGDFIPYVAGLDFQGFNRRAKSISKVIDGFLERIIDEHL 254
            DERGFK+VIQE M LAA PNLGDFIP +A LD QG +RR+K+ISK+ DGFLE I+D+HL
Sbjct: 196 FDERGFKSVIQEAMHLAATPNLGDFIPQIAALDLQGLDRRSKAISKIFDGFLEMIVDDHL 255

Query: 255 QPKIGNKTKDFVDVMLDLMNSQAREYQIERSSIKAIILDLLAAAVDTSATTISWALSELI 314
           + K  NKTKDFVDVML++MNS+  EY+IERS+IKAIILD+L AA+DTS+TTI WA+ ELI
Sbjct: 256 ESKHENKTKDFVDVMLEIMNSKETEYRIERSNIKAIILDMLVAAMDTSSTTIGWAIPELI 315

Query: 315 KHPLSMKKIQDELEKVVGLDRMVEDSDLCNLKYLDMVIKEIFRMHPPAPLLVPHESLDDC 374
           +HP  MKK+QDELEKVVG D+ VE+SDL  L+YLDMV+KEIFR+HPPAPLL+PHESL+DC
Sbjct: 316 RHPHVMKKMQDELEKVVGYDKKVEESDLDQLEYLDMVVKEIFRLHPPAPLLIPHESLEDC 375

Query: 375 TVDNFYIPKKSRIIVNAWAIGRDPNVWIDAEKFFPERFIGSQVDIKGRDFQLIPFGSGRR 434
           T++ F+IPKKSRIIVN WAIGRDP+VWID EKFFPERFIGSQ+D++GRDF+LIPFGSGRR
Sbjct: 376 TINGFHIPKKSRIIVNGWAIGRDPDVWIDPEKFFPERFIGSQIDMRGRDFELIPFGSGRR 435

Query: 435 GCPGMQMGLIVVRLVLAQLVHCFNWELPNGTLPTKLNMTEEFGLTCPRAEDLKVIPIYRI 494
           GCPG+Q+GL +VRLV+AQLVHCF+WELPNG  P KL+MTEEFGLTCPRAEDL V PIYR+
Sbjct: 436 GCPGLQLGLTIVRLVIAQLVHCFDWELPNGMSPDKLDMTEEFGLTCPRAEDLMVTPIYRL 493

BLAST of MC01g_new0383 vs. TAIR 10
Match: AT4G36220.1 (ferulic acid 5-hydroxylase 1 )

HSP 1 Score: 392.5 bits (1007), Expect = 5.0e-109
Identity = 202/510 (39.61%), Postives = 309/510 (60.59%), Query Frame = 0

Query: 7   IITILPLFLLFSSWLWVLRSHARLPPGPKGFPIFGSLHLLGKLPHRALHRLSQKYGPIMH 66
           ++ ++ LF+  S      R     PPGP+G+PI G++ ++ +L HR L  L++KYG + H
Sbjct: 19  LVIVVSLFIFIS--FITRRRRPPYPPGPRGWPIIGNMLMMDQLTHRGLANLAKKYGGLCH 78

Query: 67  LRLGLVSTIVVSSPAAAELFLKTHDHVFASRPLIQASNHISYGRKGLGFAEYGPYWRNIR 126
           LR+G +    VSSP  A   L+  D VF++RP   A ++++Y R  + FA YGP+WR +R
Sbjct: 79  LRMGFLHMYAVSSPEVARQVLQVQDSVFSNRPATIAISYLTYDRADMAFAHYGPFWRQMR 138

Query: 127 KMCTVELLSNLKVSSFRSMRSEEVGLLIDHLREAAKSDV--VVNLSSKVTSLVADMTCLM 186
           K+C +++ S  +  S+ S+R E     +D +  +   +V   +N+  ++ +L  ++T   
Sbjct: 139 KVCVMKVFSRKRAESWASVRDE-----VDKMVRSVSCNVGKPINVGEQIFALTRNITYRA 198

Query: 187 VFGKKYGDEQLDERGFKAVIQEVMQLAAAPNLGDFIPYVAGLDFQGFNRRAKSISKVIDG 246
            FG      Q DE  F  ++QE  +L  A N+ DFIPY   +D QG N+R       +DG
Sbjct: 199 AFGSACEKGQ-DE--FIRILQEFSKLFGAFNVADFIPYFGWIDPQGINKRLVKARNDLDG 258

Query: 247 FLERIIDEHLQPKIGNKT--------KDFVDVMLDLMNSQAR----------EYQIERSS 306
           F++ IIDEH++ K              D VD +L   + +A+            ++ R +
Sbjct: 259 FIDDIIDEHMKKKENQNAVDDGDVVDTDMVDDLLAFYSEEAKLVSETADLQNSIKLTRDN 318

Query: 307 IKAIILDLLAAAVDTSATTISWALSELIKHPLSMKKIQDELEKVVGLDRMVEDSDLCNLK 366
           IKAII+D++    +T A+ I WAL+EL++ P  +K++Q EL +VVGLDR VE+SD+  L 
Sbjct: 319 IKAIIMDVMFGGTETVASAIEWALTELLRSPEDLKRVQQELAEVVGLDRRVEESDIEKLT 378

Query: 367 YLDMVIKEIFRMHPPAPLLVPHESLDDCTVDNFYIPKKSRIIVNAWAIGRDPNVWIDAEK 426
           YL   +KE  RMHPP PLL+ HE+ +D ++D F+IPKKSR+++NA+AIGRDP  W D + 
Sbjct: 379 YLKCTLKETLRMHPPIPLLL-HETAEDTSIDGFFIPKKSRVMINAFAIGRDPTSWTDPDT 438

Query: 427 FFPERFIGSQV-DIKGRDFQLIPFGSGRRGCPGMQMGLIVVRLVLAQLVHCFNWELPNGT 486
           F P RF+   V D KG +F+ IPFGSGRR CPGMQ+GL  + L +A ++HCF W+LP+G 
Sbjct: 439 FRPSRFLEPGVPDFKGSNFEFIPFGSGRRSCPGMQLGLYALDLAVAHILHCFTWKLPDGM 498

Query: 487 LPTKLNMTEEFGLTCPRAEDLKVIPIYRIL 496
            P++L+M + FGLT P+A  L  +P  R++
Sbjct: 499 KPSELDMNDVFGLTAPKATRLFAVPTTRLI 517

BLAST of MC01g_new0383 vs. TAIR 10
Match: AT5G57260.1 (cytochrome P450, family 71, subfamily B, polypeptide 10 )

HSP 1 Score: 385.6 bits (989), Expect = 6.1e-107
Identity = 192/498 (38.55%), Postives = 305/498 (61.24%), Query Frame = 0

Query: 1   MASVWLIITILPLFLLFSSWLWVLRSHARLPPGPKGFPIFGSLHLLGKLPHRALHRLSQK 60
           M  +W +  IL + +L  +     R   R PP P G PI G+LH LG+LPH++L +LS+K
Sbjct: 1   MTVLWFVSLILLISILLVAVKHSKRRWVRQPPSPPGLPIIGNLHQLGELPHQSLCKLSKK 60

Query: 61  YGPIMHLRLGLVSTIVVSSPAAAELFLKTHDHVFASRPLIQASNHISYGRKGLGFAEYGP 120
           YGP+M L+LG V T++VS+P  A+  LK +D    SRP ++ +  +SY    + F+ +  
Sbjct: 61  YGPVMLLKLGRVPTVIVSTPETAKQVLKDYDLHCCSRPSLEGTRKLSYNYLDIAFSRFDD 120

Query: 121 YWRNIRKMCTVELLSNLKVSSFRSMRSEEVGLLIDHLREAAKSDVVVNLSSKVTSLVADM 180
           YW+ +RK+C  EL  N +++S + ++  E+  LID + E+A    +VNLS    SL  ++
Sbjct: 121 YWKELRKLCVEELFCNKRINSIQPIKEAEMEKLIDSIAESASQKTLVNLSDTFLSLNVNV 180

Query: 181 TCLMVFGKKYGDEQLDERGFKAVIQEVMQLAAAPNLGDFIPYVAGLD--FQGFNRRAKSI 240
            C  VFG  +    L+   F+ ++ E +++  + +  DF PYV  +   F G + R +  
Sbjct: 181 ICKAVFGVNFQGTVLNNDKFQDLVHEALEMLGSFSASDFFPYVGWIVDWFTGLHARRERS 240

Query: 241 SKVIDGFLERIIDEHLQPKIGNKTKDFVDVMLDLMNSQA--REYQIERSSIKAIILDLLA 300
            + +D F E++ID HLQ        DFVD++L L   +A     ++ R+ IKAI++++L 
Sbjct: 241 VRDLDAFYEQMIDLHLQKNREESEDDFVDLLLRLEKEEAVLGYGKLTRNHIKAILMNILL 300

Query: 301 AAVDTSATTISWALSELIKHPLSMKKIQDELEKVVGLD---RMVEDSDLCNLKYLDMVIK 360
             ++TSA T++WA++ELI++P  MKK+Q E+   +G +   R++   ++ +L YL+MVIK
Sbjct: 301 GGINTSAITMTWAMAELIRNPRVMKKVQSEIRAQIGKNNKTRIISLDEINHLSYLNMVIK 360

Query: 361 EIFRMHPPAPLLVPHESLDDCTVDNFYIPKKSRIIVNAWAIGRDPNVWIDAEKFFPERFI 420
           E  R+HP APLLVP E + +  ++ + I  K+R+ VN WAIGRDP +W D E+F PERF+
Sbjct: 361 ETCRLHPVAPLLVPREVISEFKINGYTIQPKTRLHVNVWAIGRDPEIWKDPEEFLPERFM 420

Query: 421 GSQVDIKGRDFQLIPFGSGRRGCPGMQMGLIVVRLVLAQLVHCFNWELPNGTLPTKLNMT 480
              +D+KG+D++L+PFGSGRR CP + MG+  V   LA L++ F+W+LP G     + M 
Sbjct: 421 DCDIDVKGQDYELLPFGSGRRICPAVYMGITTVEFGLANLLYHFDWKLPEGVAVEDIYMD 480

Query: 481 EEFGLTCPRAEDLKVIPI 492
           E  GLT  +  DL ++P+
Sbjct: 481 EASGLTSHKKHDLLLVPV 498

BLAST of MC01g_new0383 vs. TAIR 10
Match: AT1G13080.1 (cytochrome P450, family 71, subfamily B, polypeptide 2 )

HSP 1 Score: 377.9 bits (969), Expect = 1.3e-104
Identity = 188/476 (39.50%), Postives = 286/476 (60.08%), Query Frame = 0

Query: 26  SHARLPPGPKGFPIFGSLHLLGKLPHRALHRLSQKYGPIMHLRLGLVSTIVVSSPAAAEL 85
           S   LPP P   PI G+LH L  LPHR  H+LS KYGP++ LRLG V  +V+SS  AAE 
Sbjct: 27  SKFNLPPSPSSLPIIGNLHHLAGLPHRCFHKLSIKYGPLVFLRLGSVPVVVISSSEAAEA 86

Query: 86  FLKTHDHVFASRPLIQASNHISYGRKGLGFAEYGPYWRNIRKMCTVELLSNLKVSSFRSM 145
            LKT+D    SRP    S  +SYG K + FA YG YWR +RK+  +EL S+ KV SFR +
Sbjct: 87  VLKTNDLECCSRPKTVGSGKLSYGFKDITFAPYGEYWREVRKLAVIELFSSKKVQSFRYI 146

Query: 146 RSEEVGLLIDHLREAAKSDVVVNLSSKVTSLVADMTCLMVFGKKYGDE--QLDERGFKAV 205
           R EEV  ++  + E+A     V+LS    SL A + C +  G+ + +    +D+   + +
Sbjct: 147 REEEVDFVVKKVSESALKQSPVDLSKTFFSLTASIICRVALGQNFNESGFVIDQDRIEEL 206

Query: 206 IQEVMQLAAAPNLGDFIPYVAGLDFQGFNRRAKSISKV---IDGFLERIIDEHLQPKIGN 265
           + E  +        DF P   G       +R K I+KV   +D F + +ID+HL+P+ G 
Sbjct: 207 VTESAEALGTFTFSDFFPGGLGRFVDWLFQRHKKINKVFKELDAFYQHVIDDHLKPE-GR 266

Query: 266 KTKDFVDVMLDLMNSQ--AREYQIERSSIKAIILDLLAAAVDTSATTISWALSELIKHPL 325
           K +D V ++LD+++ Q  +  +++   ++KAI++D+  A +DTSA T+ WA++ELI++P 
Sbjct: 267 KNQDIVTLILDMIDKQEDSDSFKLNMDNLKAIVMDVFLAGIDTSAVTMIWAMTELIRNPR 326

Query: 326 SMKKIQDELEKVVGLDR-MVEDSDLCNLKYLDMVIKEIFRMHPPAPLLVPHESLDDCTVD 385
            MKK Q+ +   +GL +  + + DL  ++YL+ ++KE FR+HP  P +VP E++    + 
Sbjct: 327 VMKKAQESIRTTLGLKKERITEEDLGKVEYLNHILKETFRLHPALPFVVPRETMSHIKIQ 386

Query: 386 NFYIPKKSRIIVNAWAIGRDPNVWIDAEKFFPERFIGSQVDIKGRDFQLIPFGSGRRGCP 445
            + IP K++I +N W IGRDP  W D E+F PERF  S VD +G+ F L+PFGSGRR CP
Sbjct: 387 GYDIPPKTQIQLNVWTIGRDPKRWNDPEEFNPERFANSSVDFRGQHFDLLPFGSGRRICP 446

Query: 446 GMQMGLIVVRLVLAQLVHCFNWELPNGTLPTKLNMTEEFGLTCPRAEDLKVIPIYR 494
           GM M +  V L L  L++ F+W +P+GT    ++M E   ++  +   L+++P+ R
Sbjct: 447 GMPMAIASVELALMNLLYYFDWSMPDGTKGEDIDMEEAGNISIVKKIPLQLVPVQR 501

BLAST of MC01g_new0383 vs. TAIR 10
Match: AT5G07990.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 377.5 bits (968), Expect = 1.7e-104
Identity = 208/499 (41.68%), Postives = 287/499 (57.52%), Query Frame = 0

Query: 6   LIITILPLFLLFSSWLWVLRSHARLPPGPKGFPIFGSLHLLGKLPHRALHRLSQKYGPIM 65
           L+ T+L L L   S       + RLPPGP  +PI G+L  +G  PHR L  +   YGPI+
Sbjct: 9   LLATVLFLILRIFSHRRNRSHNNRLPPGPNPWPIIGNLPHMGTKPHRTLSAMVTTYGPIL 68

Query: 66  HLRLGLVSTIVVSSPAAAELFLKTHDHVFASRPLIQASNHISYGRKGLGFAEYGPYWRNI 125
           HLRLG V  +V +S + AE FLK HD  FASRP    + H++Y  + L FA YG  WR +
Sbjct: 69  HLRLGFVDVVVAASKSVAEQFLKIHDANFASRPPNSGAKHMAYNYQDLVFAPYGHRWRLL 128

Query: 126 RKMCTVELLSNLKVSSFRSMRSEEVGLLIDHLREAAKSDVVVNLSSKVTSLVADMTCLMV 185
           RK+ +V L S   +  F+ +R EEVG L   L         VNL   V   V +     +
Sbjct: 129 RKISSVHLFSAKALEDFKHVRQEEVGTLTRELVRVGTKP--VNLGQLVNMCVVNALGREM 188

Query: 186 FGKKY----GDEQLDERGFKAVIQEVMQLAAAPNLGDFIPYVAGLDFQGFNRRAKSISKV 245
            G++      D + DE  F++++ E+M LA   N+GDF+P +  LD QG   + K + K 
Sbjct: 189 IGRRLFGADADHKADE--FRSMVTEMMALAGVFNIGDFVPSLDWLDLQGVAGKMKRLHKR 248

Query: 246 IDGFLERIIDEHLQPKIGNKTKDFVDVMLDLMNS--QAREYQIERSSIKAIILDLLAAAV 305
            D FL  I+ EH       K  D +  ++ L  +        +  + IKA++L++  A  
Sbjct: 249 FDAFLSSILKEHEMNGQDQKHTDMLSTLISLKGTDLDGDGGSLTDTEIKALLLNMFTAGT 308

Query: 306 DTSATTISWALSELIKHPLSMKKIQDELEKVVGLDRMVEDSDLCNLKYLDMVIKEIFRMH 365
           DTSA+T+ WA++ELI+HP  M K Q+EL+ VVG DR V +SD+  L YL  VIKE FR+H
Sbjct: 309 DTSASTVDWAIAELIRHPDIMVKAQEELDIVVGRDRPVNESDIAQLPYLQAVIKENFRLH 368

Query: 366 PPAPLLVPHESLDDCTVDNFYIPKKSRIIVNAWAIGRDPNVWIDAEKFFPERFI----GS 425
           PP PL +PH + + C ++ ++IPK S ++ N WAI RDP+ W D   F PERF+     S
Sbjct: 369 PPTPLSLPHIASESCEINGYHIPKGSTLLTNIWAIARDPDQWSDPLAFKPERFLPGGEKS 428

Query: 426 QVDIKGRDFQLIPFGSGRRGCPGMQMGLIVVRLVLAQLVHCFNWELPNGTLPTKLNMTEE 485
            VD+KG DF+LIPFG+GRR C G+ +GL  ++ + A LV  F+WEL  G  P KLNM E 
Sbjct: 429 GVDVKGSDFELIPFGAGRRICAGLSLGLRTIQFLTATLVQGFDWELAGGVTPEKLNMEES 488

Query: 486 FGLTCPRAEDLKVIPIYRI 495
           +GLT  RA  L V P  R+
Sbjct: 489 YGLTLQRAVPLVVHPKPRL 503

BLAST of MC01g_new0383 vs. TAIR 10
Match: AT3G26210.1 (cytochrome P450, family 71, subfamily B, polypeptide 23 )

HSP 1 Score: 376.3 bits (965), Expect = 3.7e-104
Identity = 208/504 (41.27%), Postives = 310/504 (61.51%), Query Frame = 0

Query: 3   SVWLIITILPLFLLFSSWLWVLRSHA---RLPPGPKGFPIFGSLHLLGKLPHRALHRLSQ 62
           S++L   +L L LL  + ++  +S +   +LPPGP   PI G+LH L  LPH+ L  L +
Sbjct: 2   SIFLCFLLL-LLLLLVTIIFTRKSQSSKLKLPPGPPKLPIIGNLHYLNGLPHKCLLNLWK 61

Query: 63  KYGPIMHLRLGLVSTIVVSSPAAAELFLKTHDHVFASRPLIQASNHISYGRKGLGFAEYG 122
            +GP+M L+LG V  +V+SS  AAE  LKTHD    SRP   AS  ISY  K +GFA YG
Sbjct: 62  IHGPVMQLQLGYVPLVVISSNQAAEEVLKTHDLDCCSRPETIASKTISYNFKDIGFAPYG 121

Query: 123 PYWRNIRKMCTVELLSNLKVSSFRSMRSEEVGLLIDHLREAAKSDVVVNLSSKVTSLVAD 182
             WR +RK+  +EL S  K +SFR +R EE  LL+  L EA++    VNL   + +L A 
Sbjct: 122 EEWRALRKLAVIELFSLKKFNSFRYIREEENDLLVKKLSEASEKQSPVNLKKALFTLSAS 181

Query: 183 MTCLMVFGKK-YGDEQLDERGFKAVIQEVMQLAAAPNLGDFIPYVAGLDFQGFNRRAKSI 242
           + C + FG+  +  E +DE   + +     ++ A     +F P   G        ++KS+
Sbjct: 182 IVCRLAFGQNLHESEFIDEDSMEDLASRSEKIQAKFAFSNFFP--GGWILDKITGQSKSL 241

Query: 243 SKV---IDGFLERIIDEHLQPKIGNK---TKDFVDVMLDLMNSQARE--YQIERSSIKAI 302
           +++   +DGF  +++D+HL+P  G K   T D VDVM+D+MN Q+++  +++    IK I
Sbjct: 242 NEIFADLDGFFNQVLDDHLKP--GRKVLETPDVVDVMIDMMNKQSQDGSFKLTTDHIKGI 301

Query: 303 ILDLLAAAVDTSATTISWALSELIKHPLSMKKIQDELEKVVGLDR-MVEDSDLCNLKYLD 362
           I D+  A V+TSATTI WA++ELI++P  MKK+QDE+  V+G  R  + + DL  L Y  
Sbjct: 302 ISDIFLAGVNTSATTILWAMTELIRNPRVMKKVQDEVRTVLGEKRDRITEQDLNQLNYFK 361

Query: 363 MVIKEIFRMHPPAPLLVPHESLDDCTVDNFYIPKKSRIIVNAWAIGRDPNVWIDAEKFFP 422
           +VIKE FR+HP APLL+P E++    +  + IP+K++I+VN +AIGRDP++W + E+F P
Sbjct: 362 LVIKETFRLHPAAPLLLPREAMAKIKIQGYDIPEKTQIMVNVYAIGRDPDLWENPEEFKP 421

Query: 423 ERFIGSQVDIKGRDFQLIPFGSGRRGCPGMQMGLIVVRLVLAQLVHCFNWELPNGTLPTK 482
           ERF+ S VD +G +F+L+PFGSGRR CPGM MG+  V L L  L++ F+W LP G     
Sbjct: 422 ERFVDSSVDYRGLNFELLPFGSGRRICPGMTMGIATVELGLLNLLYFFDWGLPEGRTVKD 481

Query: 483 LNMTEEFGLTCPRAEDLKVIPIYR 494
           +++ EE  +   +   L+++P  R
Sbjct: 482 IDLEEEGAIIIGKKVSLELVPTRR 500