MC01g1673 (gene) Bitter gourd (Dali-11) v1

BLAST of MC01g1673 vs. ExPASy Swiss-Prot
Match: P29503 (Protein neuralized OS=Drosophila melanogaster OX=7227 GN=neur PE=1 SV=2)

HSP 1 Score: 67.8 bits (164), Expect = 6.4e-10
Identity = 32/55 (58.18%), Postives = 37/55 (67.27%), Query Frame = 0

Query: 742 CIICHEMQIDSLLYRCGHMCSCMKCAKELQWR---GGKCPVCGAPIDDVVRASFT 794
           C IC+E  IDS+LY CGHMC C  CA E QWR   GG+CP+C A I DV+R   T
Sbjct: 701 CTICYENPIDSVLYMCGHMCMCYDCAIE-QWRGVGGGQCPLCRAVIRDVIRTYTT 754

BLAST of MC01g1673 vs. ExPASy Swiss-Prot
Match: Q24746 (Protein neuralized OS=Drosophila virilis OX=7244 GN=neur PE=4 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 6.4e-10
Identity = 32/55 (58.18%), Postives = 37/55 (67.27%), Query Frame = 0

Query: 742 CIICHEMQIDSLLYRCGHMCSCMKCAKELQWR---GGKCPVCGAPIDDVVRASFT 794
           C IC+E  IDS+LY CGHMC C  CA E QWR   GG+CP+C A I DV+R   T
Sbjct: 694 CTICYENPIDSVLYMCGHMCMCYDCAIE-QWRGVGGGQCPLCRAVIRDVIRTYTT 747

BLAST of MC01g1673 vs. NCBI nr
Match: XP_022146713.1 (uncharacterized protein LOC111015850 [Momordica charantia])

HSP 1 Score: 1543 bits (3995), Expect = 0.0
Identity = 795/795 (100.00%), Postives = 795/795 (100.00%), Query Frame = 0

Query: 1   MASSQVEFSSSSSPFGCVLRDHNRRNANVAKFRNNLKTLVMDRLNDCISITPNPNPNSNS 60
           MASSQVEFSSSSSPFGCVLRDHNRRNANVAKFRNNLKTLVMDRLNDCISITPNPNPNSNS
Sbjct: 1   MASSQVEFSSSSSPFGCVLRDHNRRNANVAKFRNNLKTLVMDRLNDCISITPNPNPNSNS 60

Query: 61  NFRLPRTNENTANPSTPRRSSILERRPTKPLHRTLSTNIDRQRNNHHISPQTAPPPPPSP 120
           NFRLPRTNENTANPSTPRRSSILERRPTKPLHRTLSTNIDRQRNNHHISPQTAPPPPPSP
Sbjct: 61  NFRLPRTNENTANPSTPRRSSILERRPTKPLHRTLSTNIDRQRNNHHISPQTAPPPPPSP 120

Query: 121 SKETSKLGASSLVQIWEKRLNVSSNHVGLNGNGNANAKEPEPEQEQEQACSVEAGDFEDE 180
           SKETSKLGASSLVQIWEKRLNVSSNHVGLNGNGNANAKEPEPEQEQEQACSVEAGDFEDE
Sbjct: 121 SKETSKLGASSLVQIWEKRLNVSSNHVGLNGNGNANAKEPEPEQEQEQACSVEAGDFEDE 180

Query: 181 RYDAGPGSEDGFADWHSNRTSSSSSTQSQSSDAGERESERVRVADIIRRLTLTAAKPPHP 240
           RYDAGPGSEDGFADWHSNRTSSSSSTQSQSSDAGERESERVRVADIIRRLTLTAAKPPHP
Sbjct: 181 RYDAGPGSEDGFADWHSNRTSSSSSTQSQSSDAGERESERVRVADIIRRLTLTAAKPPHP 240

Query: 241 SLVEDNDQHPNESSSHPTLSLSLREQVEHKCLSHVLCSPRIRGRQAFADLLMQMERDRQK 300
           SLVEDNDQHPNESSSHPTLSLSLREQVEHKCLSHVLCSPRIRGRQAFADLLMQMERDRQK
Sbjct: 241 SLVEDNDQHPNESSSHPTLSLSLREQVEHKCLSHVLCSPRIRGRQAFADLLMQMERDRQK 300

Query: 301 ELDTLLDRRPVSKFPQRGRIQSLLRLKILKRGMVVEDEQQQRPQFVITPRENQGFSTIMH 360
           ELDTLLDRRPVSKFPQRGRIQSLLRLKILKRGMVVEDEQQQRPQFVITPRENQGFSTIMH
Sbjct: 301 ELDTLLDRRPVSKFPQRGRIQSLLRLKILKRGMVVEDEQQQRPQFVITPRENQGFSTIMH 360

Query: 361 LRERFSRVGENGLRSPIGEMLNNNNNNDDEKTQSDTDTHATCNNDNDNHQVGASASASAS 420
           LRERFSRVGENGLRSPIGEMLNNNNNNDDEKTQSDTDTHATCNNDNDNHQVGASASASAS
Sbjct: 361 LRERFSRVGENGLRSPIGEMLNNNNNNDDEKTQSDTDTHATCNNDNDNHQVGASASASAS 420

Query: 421 ACVSDSDSAIHSSSMNTVDDHPIPEDVKEQTEDQGHDPPTSEPTWQDTPNLILNSQDSIH 480
           ACVSDSDSAIHSSSMNTVDDHPIPEDVKEQTEDQGHDPPTSEPTWQDTPNLILNSQDSIH
Sbjct: 421 ACVSDSDSAIHSSSMNTVDDHPIPEDVKEQTEDQGHDPPTSEPTWQDTPNLILNSQDSIH 480

Query: 481 GWEESEAAEENYGAESDYMGTSYNWFTDISRPRSYWEDRRQSWYQQMLDSNSANDEIRQL 540
           GWEESEAAEENYGAESDYMGTSYNWFTDISRPRSYWEDRRQSWYQQMLDSNSANDEIRQL
Sbjct: 481 GWEESEAAEENYGAESDYMGTSYNWFTDISRPRSYWEDRRQSWYQQMLDSNSANDEIRQL 540

Query: 541 IERKTVSNFLTSDFRERMDKLMVSRLEQRTQQQEEYNEDNEDNDGAEEEEELWCFSEGHT 600
           IERKTVSNFLTSDFRERMDKLMVSRLEQRTQQQEEYNEDNEDNDGAEEEEELWCFSEGHT
Sbjct: 541 IERKTVSNFLTSDFRERMDKLMVSRLEQRTQQQEEYNEDNEDNDGAEEEEELWCFSEGHT 600

Query: 601 QPKSSSDNEEEEETSDYLDQSASPLQLASPSILSSWSYQDNEMGEDSNRAASMSSPQPFP 660
           QPKSSSDNEEEEETSDYLDQSASPLQLASPSILSSWSYQDNEMGEDSNRAASMSSPQPFP
Sbjct: 601 QPKSSSDNEEEEETSDYLDQSASPLQLASPSILSSWSYQDNEMGEDSNRAASMSSPQPFP 660

Query: 661 PQFSSNNQQSSLVSRSHHASIEMELIYDLRGHMEQLYQEMSELRKSIKCCMDMQLMLQQS 720
           PQFSSNNQQSSLVSRSHHASIEMELIYDLRGHMEQLYQEMSELRKSIKCCMDMQLMLQQS
Sbjct: 661 PQFSSNNQQSSLVSRSHHASIEMELIYDLRGHMEQLYQEMSELRKSIKCCMDMQLMLQQS 720

Query: 721 IKQEVVGGGRGRKSKSRKPKCCIICHEMQIDSLLYRCGHMCSCMKCAKELQWRGGKCPVC 780
           IKQEVVGGGRGRKSKSRKPKCCIICHEMQIDSLLYRCGHMCSCMKCAKELQWRGGKCPVC
Sbjct: 721 IKQEVVGGGRGRKSKSRKPKCCIICHEMQIDSLLYRCGHMCSCMKCAKELQWRGGKCPVC 780

Query: 781 GAPIDDVVRASFTHS 795
           GAPIDDVVRASFTHS
Sbjct: 781 GAPIDDVVRASFTHS 795

BLAST of MC01g1673 vs. NCBI nr
Match: XP_038877490.1 (putative uncharacterized protein DDB_G0271606 [Benincasa hispida])

HSP 1 Score: 974 bits (2517), Expect = 0.0
Identity = 584/871 (67.05%), Postives = 657/871 (75.43%), Query Frame = 0

Query: 1   MASSQVEFSSSSSPFGCVLRDHNRRN------ANVAKFRNNLKTLVMDRLNDCISITPNP 60
           MASSQVE SSSSSPFGCVLRDHNRR        +VA+FR+NLKTLVMDRLNDCI+ITPN 
Sbjct: 1   MASSQVEISSSSSPFGCVLRDHNRRREPNVAATHVARFRDNLKTLVMDRLNDCITITPNK 60

Query: 61  NPNSNSN-----------FRLPRTNENTANPSTPRRSSILERRPTKPLHRTLSTNIDRQR 120
           N N N N           FR+ +TN NT   + PRR++  +R+          T+I+  +
Sbjct: 61  NKNKNQNQNQNPNPVLPNFRVSKTNHNTTTNAAPRRANDSQRQ----------TSINDPQ 120

Query: 121 NNHHISPQTAPPPPPSPSK-ETSKLGASSLVQIWEKRLNVSSNHVGLNGNGNAN-----A 180
                   + P P    +K +TSKLGASSLVQIWEKRLNVSS++VGLN N NA+     +
Sbjct: 121 TQTQTQTTSTPTPETGTNKNQTSKLGASSLVQIWEKRLNVSSSNVGLNANANASNTAVCS 180

Query: 181 KEPEPEQEQEQACSVEAGDFEDERYDAGPGSEDGFADWHSNRTSSSS---STQSQSSDAG 240
            + E E EQEQACSVE GDFEDERYDAGPGSEDGFADWHS+RTSSSS   STQSQSSDAG
Sbjct: 181 AKQETETEQEQACSVEVGDFEDERYDAGPGSEDGFADWHSSRTSSSSPPSSTQSQSSDAG 240

Query: 241 ERESERVRVADIIRRLTLTAAKPPHPSLVEDNDQHPNESSS--HPTLSLSLREQVEH-KC 300
           ERE  RVRV DIIRRLTLTAAKPPH S VED++ HPNESSS  HPTL L  R+QVE  +C
Sbjct: 241 ERE--RVRVVDIIRRLTLTAAKPPHSSWVEDHNDHPNESSSSSHPTLIL--RDQVEPARC 300

Query: 301 LSHVLCSPRIRGRQAFADLLMQMERDRQKELDTLLDRRPVSKFPQRGRIQSLLRLKILKR 360
           LSH+LCSPRIRGRQAFADLL+Q+ERDRQ+ELD L++RR VSKF QRGRIQSLLRLKILKR
Sbjct: 301 LSHILCSPRIRGRQAFADLLLQIERDRQRELDILVERRAVSKFSQRGRIQSLLRLKILKR 360

Query: 361 GMVVEDEQQQRPQFVITPRENQGFSTIMHLRERFSRVGENGLRSPIGEMLNNNNNNDDEK 420
           GM +EDEQ+ RPQFVITPREN   STIMHLRE+FS VGENG RSPIGEMLNNN   D++K
Sbjct: 361 GMALEDEQK-RPQFVITPRENHRSSTIMHLREKFSGVGENGARSPIGEMLNNN---DEDK 420

Query: 421 TQSDTDTH----ATCNN----DNDNHQVGASASASASACVSDSDSAIHSSSMNT------ 480
            QS T+ H    AT  N    DNDN QV                  IHS+S+N       
Sbjct: 421 NQSHTNAHTPPHATNTNEKEKDNDNEQVVG----------------IHSNSINNDQILEG 480

Query: 481 VDDHPIPEDVKEQTEDQGH------------------DPPTSEPTWQDTPNLILNSQDSI 540
             +  I E  +EQ ++Q                    DPP+SE TWQD PNL L+SQDSI
Sbjct: 481 FKEEQIEEREREQEQEQKQEIEQEQEPKQEQEQGEEVDPPSSEGTWQDRPNLNLDSQDSI 540

Query: 541 HGWE---ESEAAEENYGAESDYMGTSYNWFTDISRPRSYWEDRRQSWYQQMLDSNSANDE 600
           +GWE   +SEAAEE+YGA  DY+GTSY+WF+DISRPRSYWEDRRQSWYQQMLDS+SANDE
Sbjct: 541 NGWEAEDQSEAAEESYGA--DYVGTSYDWFSDISRPRSYWEDRRQSWYQQMLDSSSANDE 600

Query: 601 IRQLIERKTVSNFLTSDFRERMDKLMVSRLEQRTQQQEEYNEDNEDNDGAEEEEELWCFS 660
           IRQLI+RKTVS FL+SDFRERMDKLMV+RLE++TQ++EEY+E NE++D  +  EELWCFS
Sbjct: 601 IRQLIQRKTVSTFLSSDFRERMDKLMVTRLERQTQEEEEYDEVNEEDDD-DRAEELWCFS 660

Query: 661 EGHTQPKSSSDNEEEE-----------ETSDYLDQSASPLQLASPSILSSWSYQ-DNEMG 720
           EG TQ KSS + EEEE           E SDYLDQS SPLQLASPSILSSWSYQ DNEMG
Sbjct: 661 EGRTQSKSSDNGEEEEDERSLISAQYHEASDYLDQSTSPLQLASPSILSSWSYQLDNEMG 720

Query: 721 EDSNRAASMSSPQPFPPQFSSNNQQ-SSLVSRSHHASIEMELIYDLRGHMEQLYQEMSEL 780
           EDSNR AS SSPQPF PQFSSNNQQ SSLVS +HH SIEMELIYDLRGHMEQLYQEMSEL
Sbjct: 721 EDSNRGASTSSPQPFQPQFSSNNQQGSSLVSTTHHPSIEMELIYDLRGHMEQLYQEMSEL 780

Query: 781 RKSIKCCMDMQLMLQQSIKQEVVGGGR-GRKSKSRKPKCCIICHEMQIDSLLYRCGHMCS 793
           RKSIKCCMDMQLMLQ SIK+  VGGGR  +K KSRKPKCCI C+ M+IDSLLYRCGHMCS
Sbjct: 781 RKSIKCCMDMQLMLQHSIKRHEVGGGRRSKKEKSRKPKCCI-CYNMEIDSLLYRCGHMCS 833

BLAST of MC01g1673 vs. NCBI nr
Match: XP_022977222.1 (uncharacterized protein LOC111477598 [Cucurbita maxima])

HSP 1 Score: 960 bits (2482), Expect = 0.0
Identity = 576/852 (67.61%), Postives = 647/852 (75.94%), Query Frame = 0

Query: 1   MASSQVEFSSSSSPFGCVLRDHNRRN------ANVAKFRNNLKTLVMDRLNDCISITPNP 60
           MASS+VE SSS+SPFGCVLRDHNRR        +VA+FRNNLKTLVMDRLNDCI+ITPN 
Sbjct: 1   MASSEVEISSSASPFGCVLRDHNRRREPNVTATHVARFRNNLKTLVMDRLNDCITITPNR 60

Query: 61  NPNSNSN-----FRLPRTNENTANPSTPRRSSILERRPTKPLHRTLSTNIDR-QRNNHHI 120
           N N N N     FR PRTN ++A    PRRS+  +         TLST I+  Q NN++ 
Sbjct: 61  NQNHNPNPVIPNFRGPRTNHDSA----PRRSNPCQ---------TLSTIINHPQNNNNNN 120

Query: 121 SPQ--TAPPPPPSPSKETSKLGASSLVQIWEKRLNVSSNHVGLNGNGNANAK------EP 180
           +PQ  T P P     K  S   ASSLVQIWEKRLNVSS++VGLN N NANA       + 
Sbjct: 121 NPQIRTTPTPETGTDKNHSSKLASSLVQIWEKRLNVSSSNVGLNANANANATPSVCSVKQ 180

Query: 181 EPEQEQEQACSVEAGDFEDERYDAGPGSEDGFADWHSNRTSSSS---STQSQSSDAGERE 240
           E EQEQEQACS+EAGDF DERYDAG GSEDGFADWHS+RTSSSS   STQSQ SDA ERE
Sbjct: 181 ETEQEQEQACSLEAGDFSDERYDAGLGSEDGFADWHSSRTSSSSPPSSTQSQISDARERE 240

Query: 241 SERVRVADIIRRLTLTAAKPPHPSLVEDNDQHPNESSSHPTLSLSLREQVEHKCLSHVLC 300
             RVRV DIIRRLTLTAAKPPH S VEDND H +ESSS+PTL L  R QVE KCLSH+L 
Sbjct: 241 --RVRVVDIIRRLTLTAAKPPHSSWVEDND-HSSESSSNPTLIL--RYQVEPKCLSHILY 300

Query: 301 SPRIRGRQAFADLLMQMERDRQKELDTLLDRRPVSKFPQRGRIQSLLRLKILKRGMVVED 360
           SPRIRGRQAFADLL+Q+ERDRQ+EL+ L++RR VSKFPQRGRIQSLLRLKIL+RGM +ED
Sbjct: 301 SPRIRGRQAFADLLLQIERDRQRELEALVERRAVSKFPQRGRIQSLLRLKILQRGMALED 360

Query: 361 EQQQRPQFVITPRENQGFSTIMHLRERFSRVGENGLRSPIGEMLNNNNNNDDEKTQSDTD 420
           EQ+ RP+FVITPR N    TI HLRERFS  GENG RSPIGEML+NN   DD+K Q DTD
Sbjct: 361 EQK-RPKFVITPRANHRAYTISHLRERFSGAGENGARSPIGEMLDNN---DDDKNQLDTD 420

Query: 421 --THATCNNDNDNHQVGASASASASACVSDSDSAIHSSSMNTVDDHPIPEDVKEQTEDQG 480
             THAT  NDNDN                D+D+   S++   V  +PIPED  E+  ++ 
Sbjct: 421 AHTHATNANDNDN----------------DNDNDKDSNNQQVVGINPIPEDFNEEEIEEQ 480

Query: 481 H-----------DPPTSEPTWQDTPNLILNSQDSIHGWE---ESEAAEENYGAESDYMGT 540
                       DPP+SE  WQD PNL L+SQDSI+GWE    SEAAEE+Y  + +Y+GT
Sbjct: 481 EPVQEPVPEPEVDPPSSEGRWQDRPNLNLDSQDSINGWEAEDHSEAAEESY--DENYLGT 540

Query: 541 SYNWFTDISRPRSYWEDRRQSWYQQMLDSNSANDEIRQLIERKTVSNFLTSDFRERMDKL 600
           SY+WF DISRPRSYWEDRR+SWYQQMLDSNSAN+EIRQLIERKTVSNFL+S+FRERMDKL
Sbjct: 541 SYDWFADISRPRSYWEDRRKSWYQQMLDSNSANEEIRQLIERKTVSNFLSSEFRERMDKL 600

Query: 601 MVSRLEQRTQQQEEYNE--DNEDNDGAEEEEELWCFSEGHTQPKSSSDNEEEEE------ 660
           MVSRLE++TQQ+EEY++  + E+ +  EEEEELWCFSEGHTQP+SS + EEEEE      
Sbjct: 601 MVSRLERQTQQEEEYDDGAEEEEEEEEEEEEELWCFSEGHTQPESSDNEEEEEEEDRDER 660

Query: 661 ----------TSDYLDQSASPLQLASPSILSSWSYQ-DNEMGEDSNRAASMSSPQPFPPQ 720
                      SD LDQSASPLQ  SPSILSSWSYQ DNEMGEDSNR AS SSPQPF PQ
Sbjct: 661 SLISSAPYQEASDDLDQSASPLQFPSPSILSSWSYQLDNEMGEDSNRGASTSSPQPFQPQ 720

Query: 721 FSSNNQQSSLVSRSHHASIEMELIYDLRGHMEQLYQEMSELRKSIKCCMDMQLMLQQSIK 780
           FSSN Q+SS VS +HH SIEMELIYDLRGHMEQLYQEMSELRKSIKCCMDMQLMLQ SIK
Sbjct: 721 FSSNTQRSSPVSTTHHPSIEMELIYDLRGHMEQLYQEMSELRKSIKCCMDMQLMLQHSIK 780

Query: 781 QEVVGGG--RGRKSKSRKPKCCIICHEMQIDSLLYRCGHMCSCMKCAKELQWRGGKCPVC 792
           +  VGGG  + +K KSRK KCCI C++MQIDSLLYRCGHMCSCMKCAKELQWRGGKCPVC
Sbjct: 781 RHEVGGGGRKSKKEKSRKRKCCI-CYDMQIDSLLYRCGHMCSCMKCAKELQWRGGKCPVC 811

BLAST of MC01g1673 vs. NCBI nr
Match: XP_022925581.1 (trichohyalin-like [Cucurbita moschata] >KAG7034825.1 Protein neuralized, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 958 bits (2476), Expect = 0.0
Identity = 579/871 (66.48%), Postives = 648/871 (74.40%), Query Frame = 0

Query: 1   MASSQVEFSSSSSPFGCVLRDHNRRN------ANVAKFRNNLKTLVMDRLNDCISITPNP 60
           MASS+VE SSS+SPFGCVLRDHNRR        +VA+FRNNLKTLVMDRLNDCI+ITPN 
Sbjct: 1   MASSEVEISSSASPFGCVLRDHNRRREPNVTATHVARFRNNLKTLVMDRLNDCITITPNR 60

Query: 61  NPNSNSN-----FRLPRTNENTANPSTPRRSSILERRPTKPLHRTLSTNIDR-QRNNHHI 120
           N N N N     FR+PRTN ++A    PRRS+  +         TLST I+  Q NN++ 
Sbjct: 61  NQNHNPNPVIPNFRVPRTNHDSA----PRRSNPCQ---------TLSTIINHPQNNNNNN 120

Query: 121 SPQTAPPPPPSPSKE---TSKLGASSLVQIWEKRLNVSSNHVGLNGNGNANA-------- 180
           +PQT   P P    +   +SKLGASSLVQIWEKRLNVSS++VGLN N NANA        
Sbjct: 121 NPQTRTTPTPQTGTDKNHSSKLGASSLVQIWEKRLNVSSSNVGLNANANANATPSVCSVK 180

Query: 181 ------KEPEPEQEQEQACSVEAGDFEDERYDAGPGSEDGFADWHSNRTSSSS---STQS 240
                 +E E EQEQEQACS+EAGDF DERYDAG GSED FADWHS+RTSSSS   STQS
Sbjct: 181 QETEQEQEQEQEQEQEQACSLEAGDFGDERYDAGLGSEDVFADWHSSRTSSSSPPSSTQS 240

Query: 241 QSSDAGERESERVRVADIIRRLTLTAAKPPHPSLVEDNDQHPNESSSHPTLSLSLREQVE 300
           Q SDA ERE  RVRV DIIRRLTLTAAKPPH S VEDND H NESSS+PTL L  R QVE
Sbjct: 241 QISDARERE--RVRVVDIIRRLTLTAAKPPHSSWVEDND-HSNESSSNPTLIL--RYQVE 300

Query: 301 HKCLSHVLCSPRIRGRQAFADLLMQMERDRQKELDTLLDRRPVSKFPQRGRIQSLLRLKI 360
            KCLSH+L SPRIRGRQAFADLL+Q+ERDRQ+EL+TL++RR VSKFPQRGRIQSLLRLKI
Sbjct: 301 PKCLSHILYSPRIRGRQAFADLLLQIERDRQRELETLVERRAVSKFPQRGRIQSLLRLKI 360

Query: 361 LKRGMVVEDEQQQRPQFVITPRENQGFSTIMHLRERFSRVGENGLRSPIGEMLNNNNNND 420
           L+RGM +EDEQ+ RP+FVITPR N    TI HLRERFS  GENG RSPIGEML+NN   D
Sbjct: 361 LQRGMALEDEQK-RPKFVITPRANHRAYTISHLRERFSGAGENGARSPIGEMLDNN---D 420

Query: 421 DEKTQSDTD--THATCNNDNDNHQVGASASASASACVSDSDSAIHSSSMNTVDDHPIPED 480
           D+K Q DTD  THAT   DNDN                  D+   S++   V  +PIPE 
Sbjct: 421 DDKNQLDTDPHTHATNTKDNDN------------------DNDKDSNNQQVVGINPIPEH 480

Query: 481 VKEQT---------------EDQGHDPPTSEPTWQDTPNLILNSQDSIHGWE---ESEAA 540
             E+                ++Q  DPP+SE  WQD PNL L+SQDSI+GWE    SEAA
Sbjct: 481 FNEEEIEEKEEEPAQEPEPEQEQEVDPPSSEGRWQDRPNLNLDSQDSINGWEAEDHSEAA 540

Query: 541 EENYGAESDYMGTSYNWFTDISRPRSYWEDRRQSWYQQMLDSNSANDEIRQLIERKTVSN 600
           EE+Y  + +Y+GTSY+WF DISRPRSYWEDRR+SWYQQMLDSNSAN+EIRQLIERKTVSN
Sbjct: 541 EESY--DENYLGTSYDWFADISRPRSYWEDRRKSWYQQMLDSNSANEEIRQLIERKTVSN 600

Query: 601 FLTSDFRERMDKLMVSRLEQRTQQQEEYNEDNEDNDGAEEEEE------LWCFSEGHTQP 660
           FL+S+FRERMDKLMVSRLE++TQQ+EEY++  E+ D  EEEEE      LWCFSEGHTQP
Sbjct: 601 FLSSEFRERMDKLMVSRLERQTQQEEEYDDGAEEEDEEEEEEEEEGEEELWCFSEGHTQP 660

Query: 661 KSSSDNEEEEE------------------TSDYLDQSASPLQLASPSILSSWSYQ-DNEM 720
           KSS + EEEEE                   SD LD SASPLQ  SPSILSSWSYQ DNEM
Sbjct: 661 KSSDNEEEEEEEEEDRDERSLISSAQYQEASDDLDPSASPLQFPSPSILSSWSYQLDNEM 720

Query: 721 GEDSNRAASMSSPQPFPPQFSSNNQQSSLVSRSHHASIEMELIYDLRGHMEQLYQEMSEL 780
           GEDSNR AS SSPQPF PQFSSN Q+SS VS +HH SIEMELIYDLRGHMEQLYQEMSEL
Sbjct: 721 GEDSNRGASTSSPQPFQPQFSSNTQRSSPVSTTHHPSIEMELIYDLRGHMEQLYQEMSEL 780

Query: 781 RKSIKCCMDMQLMLQQSIKQEVVGGG--RGRKSKSRKPKCCIICHEMQIDSLLYRCGHMC 792
           RKSIKCCMDMQLMLQ SIK+  VGGG  + +K KSRK KCCI C++MQIDSLLYRCGHMC
Sbjct: 781 RKSIKCCMDMQLMLQHSIKRHEVGGGGRKSKKEKSRKRKCCI-CYDMQIDSLLYRCGHMC 828

BLAST of MC01g1673 vs. NCBI nr
Match: KAG6581531.1 (Protein neuralized, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 958 bits (2476), Expect = 0.0
Identity = 579/871 (66.48%), Postives = 648/871 (74.40%), Query Frame = 0

Query: 1    MASSQVEFSSSSSPFGCVLRDHNRRN------ANVAKFRNNLKTLVMDRLNDCISITPNP 60
            MASS+VE SSS+SPFGCVLRDHNRR        +VA+FRNNLKTLVMDRLNDCI+ITPN 
Sbjct: 515  MASSEVEISSSASPFGCVLRDHNRRREPNVTATHVARFRNNLKTLVMDRLNDCITITPNR 574

Query: 61   NPNSNSN-----FRLPRTNENTANPSTPRRSSILERRPTKPLHRTLSTNIDR-QRNNHHI 120
            N N N N     FR+PRTN ++A    PRRS+  +         TLST I+  Q NN++ 
Sbjct: 575  NQNHNPNPVIPNFRVPRTNHDSA----PRRSNPCQ---------TLSTIINHPQNNNNNN 634

Query: 121  SPQTAPPPPPSPSKE---TSKLGASSLVQIWEKRLNVSSNHVGLNGNGNANA-------- 180
            +PQT   P P    +   +SKLGASSLVQIWEKRLNVSS++VGLN N NANA        
Sbjct: 635  NPQTRTTPTPQTGTDKNHSSKLGASSLVQIWEKRLNVSSSNVGLNANANANATPSVCSVK 694

Query: 181  ------KEPEPEQEQEQACSVEAGDFEDERYDAGPGSEDGFADWHSNRTSSSS---STQS 240
                  +E E EQEQEQACS+EAGDF DERYDAG GSED FADWHS+RTSSSS   STQS
Sbjct: 695  QETEQEQEQEQEQEQEQACSLEAGDFGDERYDAGLGSEDVFADWHSSRTSSSSPPSSTQS 754

Query: 241  QSSDAGERESERVRVADIIRRLTLTAAKPPHPSLVEDNDQHPNESSSHPTLSLSLREQVE 300
            Q SDA ERE  RVRV DIIRRLTLTAAKPPH S VEDND H NESSS+PTL L  R QVE
Sbjct: 755  QISDARERE--RVRVVDIIRRLTLTAAKPPHSSWVEDND-HSNESSSNPTLIL--RYQVE 814

Query: 301  HKCLSHVLCSPRIRGRQAFADLLMQMERDRQKELDTLLDRRPVSKFPQRGRIQSLLRLKI 360
             KCLSH+L SPRIRGRQAFADLL+Q+ERDRQ+EL+TL++RR VSKFPQRGRIQSLLRLKI
Sbjct: 815  PKCLSHILYSPRIRGRQAFADLLLQIERDRQRELETLVERRAVSKFPQRGRIQSLLRLKI 874

Query: 361  LKRGMVVEDEQQQRPQFVITPRENQGFSTIMHLRERFSRVGENGLRSPIGEMLNNNNNND 420
            L+RGM +EDEQ+ RP+FVITPR N    TI HLRERFS  GENG RSPIGEML+NN   D
Sbjct: 875  LQRGMALEDEQK-RPKFVITPRANHRAYTISHLRERFSGAGENGARSPIGEMLDNN---D 934

Query: 421  DEKTQSDTD--THATCNNDNDNHQVGASASASASACVSDSDSAIHSSSMNTVDDHPIPED 480
            D+K Q DTD  THAT   DNDN                  D+   S++   V  +PIPE 
Sbjct: 935  DDKNQLDTDPHTHATNTKDNDN------------------DNDKDSNNQQVVGINPIPEH 994

Query: 481  VKEQT---------------EDQGHDPPTSEPTWQDTPNLILNSQDSIHGWE---ESEAA 540
              E+                ++Q  DPP+SE  WQD PNL L+SQDSI+GWE    SEAA
Sbjct: 995  FNEEEIEEKEEEPAQEPEPEQEQEVDPPSSEGRWQDRPNLNLDSQDSINGWEAEDHSEAA 1054

Query: 541  EENYGAESDYMGTSYNWFTDISRPRSYWEDRRQSWYQQMLDSNSANDEIRQLIERKTVSN 600
            EE+Y  + +Y+GTSY+WF DISRPRSYWEDRR+SWYQQMLDSNSAN+EIRQLIERKTVSN
Sbjct: 1055 EESY--DENYLGTSYDWFADISRPRSYWEDRRKSWYQQMLDSNSANEEIRQLIERKTVSN 1114

Query: 601  FLTSDFRERMDKLMVSRLEQRTQQQEEYNEDNEDNDGAEEEEE------LWCFSEGHTQP 660
            FL+S+FRERMDKLMVSRLE++TQQ+EEY++  E+ D  EEEEE      LWCFSEGHTQP
Sbjct: 1115 FLSSEFRERMDKLMVSRLERQTQQEEEYDDGAEEEDEEEEEEEEEGEEELWCFSEGHTQP 1174

Query: 661  KSSSDNEEEEE------------------TSDYLDQSASPLQLASPSILSSWSYQ-DNEM 720
            KSS + EEEEE                   SD LD SASPLQ  SPSILSSWSYQ DNEM
Sbjct: 1175 KSSDNEEEEEEEEEDRDERSLISSAQYQEASDDLDPSASPLQFPSPSILSSWSYQLDNEM 1234

Query: 721  GEDSNRAASMSSPQPFPPQFSSNNQQSSLVSRSHHASIEMELIYDLRGHMEQLYQEMSEL 780
            GEDSNR AS SSPQPF PQFSSN Q+SS VS +HH SIEMELIYDLRGHMEQLYQEMSEL
Sbjct: 1235 GEDSNRGASTSSPQPFQPQFSSNTQRSSPVSTTHHPSIEMELIYDLRGHMEQLYQEMSEL 1294

Query: 781  RKSIKCCMDMQLMLQQSIKQEVVGGG--RGRKSKSRKPKCCIICHEMQIDSLLYRCGHMC 792
            RKSIKCCMDMQLMLQ SIK+  VGGG  + +K KSRK KCCI C++MQIDSLLYRCGHMC
Sbjct: 1295 RKSIKCCMDMQLMLQHSIKRHEVGGGGRKSKKEKSRKRKCCI-CYDMQIDSLLYRCGHMC 1342

BLAST of MC01g1673 vs. ExPASy TrEMBL
Match: A0A6J1CZA0 (uncharacterized protein LOC111015850 OS=Momordica charantia OX=3673 GN=LOC111015850 PE=4 SV=1)

HSP 1 Score: 1543 bits (3995), Expect = 0.0
Identity = 795/795 (100.00%), Postives = 795/795 (100.00%), Query Frame = 0

Query: 1   MASSQVEFSSSSSPFGCVLRDHNRRNANVAKFRNNLKTLVMDRLNDCISITPNPNPNSNS 60
           MASSQVEFSSSSSPFGCVLRDHNRRNANVAKFRNNLKTLVMDRLNDCISITPNPNPNSNS
Sbjct: 1   MASSQVEFSSSSSPFGCVLRDHNRRNANVAKFRNNLKTLVMDRLNDCISITPNPNPNSNS 60

Query: 61  NFRLPRTNENTANPSTPRRSSILERRPTKPLHRTLSTNIDRQRNNHHISPQTAPPPPPSP 120
           NFRLPRTNENTANPSTPRRSSILERRPTKPLHRTLSTNIDRQRNNHHISPQTAPPPPPSP
Sbjct: 61  NFRLPRTNENTANPSTPRRSSILERRPTKPLHRTLSTNIDRQRNNHHISPQTAPPPPPSP 120

Query: 121 SKETSKLGASSLVQIWEKRLNVSSNHVGLNGNGNANAKEPEPEQEQEQACSVEAGDFEDE 180
           SKETSKLGASSLVQIWEKRLNVSSNHVGLNGNGNANAKEPEPEQEQEQACSVEAGDFEDE
Sbjct: 121 SKETSKLGASSLVQIWEKRLNVSSNHVGLNGNGNANAKEPEPEQEQEQACSVEAGDFEDE 180

Query: 181 RYDAGPGSEDGFADWHSNRTSSSSSTQSQSSDAGERESERVRVADIIRRLTLTAAKPPHP 240
           RYDAGPGSEDGFADWHSNRTSSSSSTQSQSSDAGERESERVRVADIIRRLTLTAAKPPHP
Sbjct: 181 RYDAGPGSEDGFADWHSNRTSSSSSTQSQSSDAGERESERVRVADIIRRLTLTAAKPPHP 240

Query: 241 SLVEDNDQHPNESSSHPTLSLSLREQVEHKCLSHVLCSPRIRGRQAFADLLMQMERDRQK 300
           SLVEDNDQHPNESSSHPTLSLSLREQVEHKCLSHVLCSPRIRGRQAFADLLMQMERDRQK
Sbjct: 241 SLVEDNDQHPNESSSHPTLSLSLREQVEHKCLSHVLCSPRIRGRQAFADLLMQMERDRQK 300

Query: 301 ELDTLLDRRPVSKFPQRGRIQSLLRLKILKRGMVVEDEQQQRPQFVITPRENQGFSTIMH 360
           ELDTLLDRRPVSKFPQRGRIQSLLRLKILKRGMVVEDEQQQRPQFVITPRENQGFSTIMH
Sbjct: 301 ELDTLLDRRPVSKFPQRGRIQSLLRLKILKRGMVVEDEQQQRPQFVITPRENQGFSTIMH 360

Query: 361 LRERFSRVGENGLRSPIGEMLNNNNNNDDEKTQSDTDTHATCNNDNDNHQVGASASASAS 420
           LRERFSRVGENGLRSPIGEMLNNNNNNDDEKTQSDTDTHATCNNDNDNHQVGASASASAS
Sbjct: 361 LRERFSRVGENGLRSPIGEMLNNNNNNDDEKTQSDTDTHATCNNDNDNHQVGASASASAS 420

Query: 421 ACVSDSDSAIHSSSMNTVDDHPIPEDVKEQTEDQGHDPPTSEPTWQDTPNLILNSQDSIH 480
           ACVSDSDSAIHSSSMNTVDDHPIPEDVKEQTEDQGHDPPTSEPTWQDTPNLILNSQDSIH
Sbjct: 421 ACVSDSDSAIHSSSMNTVDDHPIPEDVKEQTEDQGHDPPTSEPTWQDTPNLILNSQDSIH 480

Query: 481 GWEESEAAEENYGAESDYMGTSYNWFTDISRPRSYWEDRRQSWYQQMLDSNSANDEIRQL 540
           GWEESEAAEENYGAESDYMGTSYNWFTDISRPRSYWEDRRQSWYQQMLDSNSANDEIRQL
Sbjct: 481 GWEESEAAEENYGAESDYMGTSYNWFTDISRPRSYWEDRRQSWYQQMLDSNSANDEIRQL 540

Query: 541 IERKTVSNFLTSDFRERMDKLMVSRLEQRTQQQEEYNEDNEDNDGAEEEEELWCFSEGHT 600
           IERKTVSNFLTSDFRERMDKLMVSRLEQRTQQQEEYNEDNEDNDGAEEEEELWCFSEGHT
Sbjct: 541 IERKTVSNFLTSDFRERMDKLMVSRLEQRTQQQEEYNEDNEDNDGAEEEEELWCFSEGHT 600

Query: 601 QPKSSSDNEEEEETSDYLDQSASPLQLASPSILSSWSYQDNEMGEDSNRAASMSSPQPFP 660
           QPKSSSDNEEEEETSDYLDQSASPLQLASPSILSSWSYQDNEMGEDSNRAASMSSPQPFP
Sbjct: 601 QPKSSSDNEEEEETSDYLDQSASPLQLASPSILSSWSYQDNEMGEDSNRAASMSSPQPFP 660

Query: 661 PQFSSNNQQSSLVSRSHHASIEMELIYDLRGHMEQLYQEMSELRKSIKCCMDMQLMLQQS 720
           PQFSSNNQQSSLVSRSHHASIEMELIYDLRGHMEQLYQEMSELRKSIKCCMDMQLMLQQS
Sbjct: 661 PQFSSNNQQSSLVSRSHHASIEMELIYDLRGHMEQLYQEMSELRKSIKCCMDMQLMLQQS 720

Query: 721 IKQEVVGGGRGRKSKSRKPKCCIICHEMQIDSLLYRCGHMCSCMKCAKELQWRGGKCPVC 780
           IKQEVVGGGRGRKSKSRKPKCCIICHEMQIDSLLYRCGHMCSCMKCAKELQWRGGKCPVC
Sbjct: 721 IKQEVVGGGRGRKSKSRKPKCCIICHEMQIDSLLYRCGHMCSCMKCAKELQWRGGKCPVC 780

Query: 781 GAPIDDVVRASFTHS 795
           GAPIDDVVRASFTHS
Sbjct: 781 GAPIDDVVRASFTHS 795

BLAST of MC01g1673 vs. ExPASy TrEMBL
Match: A0A6J1IHV7 (uncharacterized protein LOC111477598 OS=Cucurbita maxima OX=3661 GN=LOC111477598 PE=4 SV=1)

HSP 1 Score: 960 bits (2482), Expect = 0.0
Identity = 576/852 (67.61%), Postives = 647/852 (75.94%), Query Frame = 0

Query: 1   MASSQVEFSSSSSPFGCVLRDHNRRN------ANVAKFRNNLKTLVMDRLNDCISITPNP 60
           MASS+VE SSS+SPFGCVLRDHNRR        +VA+FRNNLKTLVMDRLNDCI+ITPN 
Sbjct: 1   MASSEVEISSSASPFGCVLRDHNRRREPNVTATHVARFRNNLKTLVMDRLNDCITITPNR 60

Query: 61  NPNSNSN-----FRLPRTNENTANPSTPRRSSILERRPTKPLHRTLSTNIDR-QRNNHHI 120
           N N N N     FR PRTN ++A    PRRS+  +         TLST I+  Q NN++ 
Sbjct: 61  NQNHNPNPVIPNFRGPRTNHDSA----PRRSNPCQ---------TLSTIINHPQNNNNNN 120

Query: 121 SPQ--TAPPPPPSPSKETSKLGASSLVQIWEKRLNVSSNHVGLNGNGNANAK------EP 180
           +PQ  T P P     K  S   ASSLVQIWEKRLNVSS++VGLN N NANA       + 
Sbjct: 121 NPQIRTTPTPETGTDKNHSSKLASSLVQIWEKRLNVSSSNVGLNANANANATPSVCSVKQ 180

Query: 181 EPEQEQEQACSVEAGDFEDERYDAGPGSEDGFADWHSNRTSSSS---STQSQSSDAGERE 240
           E EQEQEQACS+EAGDF DERYDAG GSEDGFADWHS+RTSSSS   STQSQ SDA ERE
Sbjct: 181 ETEQEQEQACSLEAGDFSDERYDAGLGSEDGFADWHSSRTSSSSPPSSTQSQISDARERE 240

Query: 241 SERVRVADIIRRLTLTAAKPPHPSLVEDNDQHPNESSSHPTLSLSLREQVEHKCLSHVLC 300
             RVRV DIIRRLTLTAAKPPH S VEDND H +ESSS+PTL L  R QVE KCLSH+L 
Sbjct: 241 --RVRVVDIIRRLTLTAAKPPHSSWVEDND-HSSESSSNPTLIL--RYQVEPKCLSHILY 300

Query: 301 SPRIRGRQAFADLLMQMERDRQKELDTLLDRRPVSKFPQRGRIQSLLRLKILKRGMVVED 360
           SPRIRGRQAFADLL+Q+ERDRQ+EL+ L++RR VSKFPQRGRIQSLLRLKIL+RGM +ED
Sbjct: 301 SPRIRGRQAFADLLLQIERDRQRELEALVERRAVSKFPQRGRIQSLLRLKILQRGMALED 360

Query: 361 EQQQRPQFVITPRENQGFSTIMHLRERFSRVGENGLRSPIGEMLNNNNNNDDEKTQSDTD 420
           EQ+ RP+FVITPR N    TI HLRERFS  GENG RSPIGEML+NN   DD+K Q DTD
Sbjct: 361 EQK-RPKFVITPRANHRAYTISHLRERFSGAGENGARSPIGEMLDNN---DDDKNQLDTD 420

Query: 421 --THATCNNDNDNHQVGASASASASACVSDSDSAIHSSSMNTVDDHPIPEDVKEQTEDQG 480
             THAT  NDNDN                D+D+   S++   V  +PIPED  E+  ++ 
Sbjct: 421 AHTHATNANDNDN----------------DNDNDKDSNNQQVVGINPIPEDFNEEEIEEQ 480

Query: 481 H-----------DPPTSEPTWQDTPNLILNSQDSIHGWE---ESEAAEENYGAESDYMGT 540
                       DPP+SE  WQD PNL L+SQDSI+GWE    SEAAEE+Y  + +Y+GT
Sbjct: 481 EPVQEPVPEPEVDPPSSEGRWQDRPNLNLDSQDSINGWEAEDHSEAAEESY--DENYLGT 540

Query: 541 SYNWFTDISRPRSYWEDRRQSWYQQMLDSNSANDEIRQLIERKTVSNFLTSDFRERMDKL 600
           SY+WF DISRPRSYWEDRR+SWYQQMLDSNSAN+EIRQLIERKTVSNFL+S+FRERMDKL
Sbjct: 541 SYDWFADISRPRSYWEDRRKSWYQQMLDSNSANEEIRQLIERKTVSNFLSSEFRERMDKL 600

Query: 601 MVSRLEQRTQQQEEYNE--DNEDNDGAEEEEELWCFSEGHTQPKSSSDNEEEEE------ 660
           MVSRLE++TQQ+EEY++  + E+ +  EEEEELWCFSEGHTQP+SS + EEEEE      
Sbjct: 601 MVSRLERQTQQEEEYDDGAEEEEEEEEEEEEELWCFSEGHTQPESSDNEEEEEEEDRDER 660

Query: 661 ----------TSDYLDQSASPLQLASPSILSSWSYQ-DNEMGEDSNRAASMSSPQPFPPQ 720
                      SD LDQSASPLQ  SPSILSSWSYQ DNEMGEDSNR AS SSPQPF PQ
Sbjct: 661 SLISSAPYQEASDDLDQSASPLQFPSPSILSSWSYQLDNEMGEDSNRGASTSSPQPFQPQ 720

Query: 721 FSSNNQQSSLVSRSHHASIEMELIYDLRGHMEQLYQEMSELRKSIKCCMDMQLMLQQSIK 780
           FSSN Q+SS VS +HH SIEMELIYDLRGHMEQLYQEMSELRKSIKCCMDMQLMLQ SIK
Sbjct: 721 FSSNTQRSSPVSTTHHPSIEMELIYDLRGHMEQLYQEMSELRKSIKCCMDMQLMLQHSIK 780

Query: 781 QEVVGGG--RGRKSKSRKPKCCIICHEMQIDSLLYRCGHMCSCMKCAKELQWRGGKCPVC 792
           +  VGGG  + +K KSRK KCCI C++MQIDSLLYRCGHMCSCMKCAKELQWRGGKCPVC
Sbjct: 781 RHEVGGGGRKSKKEKSRKRKCCI-CYDMQIDSLLYRCGHMCSCMKCAKELQWRGGKCPVC 811

BLAST of MC01g1673 vs. ExPASy TrEMBL
Match: A0A6J1ECL3 (trichohyalin-like OS=Cucurbita moschata OX=3662 GN=LOC111432973 PE=4 SV=1)

HSP 1 Score: 958 bits (2476), Expect = 0.0
Identity = 579/871 (66.48%), Postives = 648/871 (74.40%), Query Frame = 0

Query: 1   MASSQVEFSSSSSPFGCVLRDHNRRN------ANVAKFRNNLKTLVMDRLNDCISITPNP 60
           MASS+VE SSS+SPFGCVLRDHNRR        +VA+FRNNLKTLVMDRLNDCI+ITPN 
Sbjct: 1   MASSEVEISSSASPFGCVLRDHNRRREPNVTATHVARFRNNLKTLVMDRLNDCITITPNR 60

Query: 61  NPNSNSN-----FRLPRTNENTANPSTPRRSSILERRPTKPLHRTLSTNIDR-QRNNHHI 120
           N N N N     FR+PRTN ++A    PRRS+  +         TLST I+  Q NN++ 
Sbjct: 61  NQNHNPNPVIPNFRVPRTNHDSA----PRRSNPCQ---------TLSTIINHPQNNNNNN 120

Query: 121 SPQTAPPPPPSPSKE---TSKLGASSLVQIWEKRLNVSSNHVGLNGNGNANA-------- 180
           +PQT   P P    +   +SKLGASSLVQIWEKRLNVSS++VGLN N NANA        
Sbjct: 121 NPQTRTTPTPQTGTDKNHSSKLGASSLVQIWEKRLNVSSSNVGLNANANANATPSVCSVK 180

Query: 181 ------KEPEPEQEQEQACSVEAGDFEDERYDAGPGSEDGFADWHSNRTSSSS---STQS 240
                 +E E EQEQEQACS+EAGDF DERYDAG GSED FADWHS+RTSSSS   STQS
Sbjct: 181 QETEQEQEQEQEQEQEQACSLEAGDFGDERYDAGLGSEDVFADWHSSRTSSSSPPSSTQS 240

Query: 241 QSSDAGERESERVRVADIIRRLTLTAAKPPHPSLVEDNDQHPNESSSHPTLSLSLREQVE 300
           Q SDA ERE  RVRV DIIRRLTLTAAKPPH S VEDND H NESSS+PTL L  R QVE
Sbjct: 241 QISDARERE--RVRVVDIIRRLTLTAAKPPHSSWVEDND-HSNESSSNPTLIL--RYQVE 300

Query: 301 HKCLSHVLCSPRIRGRQAFADLLMQMERDRQKELDTLLDRRPVSKFPQRGRIQSLLRLKI 360
            KCLSH+L SPRIRGRQAFADLL+Q+ERDRQ+EL+TL++RR VSKFPQRGRIQSLLRLKI
Sbjct: 301 PKCLSHILYSPRIRGRQAFADLLLQIERDRQRELETLVERRAVSKFPQRGRIQSLLRLKI 360

Query: 361 LKRGMVVEDEQQQRPQFVITPRENQGFSTIMHLRERFSRVGENGLRSPIGEMLNNNNNND 420
           L+RGM +EDEQ+ RP+FVITPR N    TI HLRERFS  GENG RSPIGEML+NN   D
Sbjct: 361 LQRGMALEDEQK-RPKFVITPRANHRAYTISHLRERFSGAGENGARSPIGEMLDNN---D 420

Query: 421 DEKTQSDTD--THATCNNDNDNHQVGASASASASACVSDSDSAIHSSSMNTVDDHPIPED 480
           D+K Q DTD  THAT   DNDN                  D+   S++   V  +PIPE 
Sbjct: 421 DDKNQLDTDPHTHATNTKDNDN------------------DNDKDSNNQQVVGINPIPEH 480

Query: 481 VKEQT---------------EDQGHDPPTSEPTWQDTPNLILNSQDSIHGWE---ESEAA 540
             E+                ++Q  DPP+SE  WQD PNL L+SQDSI+GWE    SEAA
Sbjct: 481 FNEEEIEEKEEEPAQEPEPEQEQEVDPPSSEGRWQDRPNLNLDSQDSINGWEAEDHSEAA 540

Query: 541 EENYGAESDYMGTSYNWFTDISRPRSYWEDRRQSWYQQMLDSNSANDEIRQLIERKTVSN 600
           EE+Y  + +Y+GTSY+WF DISRPRSYWEDRR+SWYQQMLDSNSAN+EIRQLIERKTVSN
Sbjct: 541 EESY--DENYLGTSYDWFADISRPRSYWEDRRKSWYQQMLDSNSANEEIRQLIERKTVSN 600

Query: 601 FLTSDFRERMDKLMVSRLEQRTQQQEEYNEDNEDNDGAEEEEE------LWCFSEGHTQP 660
           FL+S+FRERMDKLMVSRLE++TQQ+EEY++  E+ D  EEEEE      LWCFSEGHTQP
Sbjct: 601 FLSSEFRERMDKLMVSRLERQTQQEEEYDDGAEEEDEEEEEEEEEGEEELWCFSEGHTQP 660

Query: 661 KSSSDNEEEEE------------------TSDYLDQSASPLQLASPSILSSWSYQ-DNEM 720
           KSS + EEEEE                   SD LD SASPLQ  SPSILSSWSYQ DNEM
Sbjct: 661 KSSDNEEEEEEEEEDRDERSLISSAQYQEASDDLDPSASPLQFPSPSILSSWSYQLDNEM 720

Query: 721 GEDSNRAASMSSPQPFPPQFSSNNQQSSLVSRSHHASIEMELIYDLRGHMEQLYQEMSEL 780
           GEDSNR AS SSPQPF PQFSSN Q+SS VS +HH SIEMELIYDLRGHMEQLYQEMSEL
Sbjct: 721 GEDSNRGASTSSPQPFQPQFSSNTQRSSPVSTTHHPSIEMELIYDLRGHMEQLYQEMSEL 780

Query: 781 RKSIKCCMDMQLMLQQSIKQEVVGGG--RGRKSKSRKPKCCIICHEMQIDSLLYRCGHMC 792
           RKSIKCCMDMQLMLQ SIK+  VGGG  + +K KSRK KCCI C++MQIDSLLYRCGHMC
Sbjct: 781 RKSIKCCMDMQLMLQHSIKRHEVGGGGRKSKKEKSRKRKCCI-CYDMQIDSLLYRCGHMC 828

BLAST of MC01g1673 vs. ExPASy TrEMBL
Match: A0A1S3AYB9 (uncharacterized protein LOC103484234 OS=Cucumis melo OX=3656 GN=LOC103484234 PE=4 SV=1)

HSP 1 Score: 938 bits (2425), Expect = 0.0
Identity = 572/845 (67.69%), Postives = 641/845 (75.86%), Query Frame = 0

Query: 1   MASSQVEFSSSSSPFGCVLRDHNRRN------ANVAKFRNNLKTLVMDRLNDCISITPNP 60
           MASSQVEFSSSSSPFGCVLRDHNRR        +VA+FRNNLKTLVMDRLNDCI+ITPN 
Sbjct: 1   MASSQVEFSSSSSPFGCVLRDHNRRREPSVTATHVARFRNNLKTLVMDRLNDCITITPNQ 60

Query: 61  NPNSN-SNFRLPRTNENTANPSTPRRSSILERRPTK-PLHRTLSTNIDRQRNNHHISPQT 120
           N N   +NF + +TN +T   ST R     +   T  P  +T ST               
Sbjct: 61  NTNPVLTNFPVTKTNHDT---STKRSDDSQQHASTNDPQTQTTST--------------P 120

Query: 121 APP-PPPSPSK-ETSKLGASSLVQIWEKRLNVSSNHVGLNGNGNANA------KEPEPEQ 180
           APP P  SP+K +TSKLGASSLVQIWEKRLNVSS+++GLN N NAN       +E E EQ
Sbjct: 121 APPIPETSPNKNQTSKLGASSLVQIWEKRLNVSSSNIGLNANANANTAVCSTKQETEAEQ 180

Query: 181 EQEQACSVEAGDFEDERYDAGPGSEDGFADWHSNRTSSSS---STQSQSSDAGERESERV 240
           EQEQACSVEAGDFEDERYDAGPGS+DGFADWHS+RTSSSS   STQSQSSDAGERE  RV
Sbjct: 181 EQEQACSVEAGDFEDERYDAGPGSDDGFADWHSSRTSSSSPPSSTQSQSSDAGERE--RV 240

Query: 241 RVADIIRRLTLTAAKPPHPSLVEDNDQHPNESSS-HPTLSLSLREQVEHKCLSHVLCSPR 300
           RV DIIRRLTLTAAKPPH S VED++  PNESSS HPTL    ++QVE KCLSH+LCSPR
Sbjct: 241 RVVDIIRRLTLTAAKPPHSSWVEDHNDQPNESSSLHPTLIP--KDQVEPKCLSHILCSPR 300

Query: 301 IRGRQAFADLLMQMERDRQKELDTLLDRRPVSKFPQRGRIQSLLRLKILKRGMVVEDEQQ 360
           IRGRQAFADLL+Q+ERDRQ+ELD L++RR VSKFPQRGRIQSLLRLKILKRGM +EDEQ+
Sbjct: 301 IRGRQAFADLLLQIERDRQRELDVLVERRAVSKFPQRGRIQSLLRLKILKRGMALEDEQK 360

Query: 361 QRPQFVITPRENQGFSTIMHLRERFSRVGENGLRSPIGEMLNNNNNNDDEKTQSDTDT-- 420
            RPQFVITPREN    TIMHLRERFS VGENG +SP GEMLN+N   DD+KTQ DT+   
Sbjct: 361 -RPQFVITPRENHRSFTIMHLRERFSGVGENGAKSPRGEMLNDN---DDDKTQPDTNANT 420

Query: 421 ----HATCNN----DNDNHQVGASASASASACVSDSDSAIHSSSMNTVDDHPIPEDVKEQ 480
               H    N    DNDN QV    S +     +D+D  I       ++      D + +
Sbjct: 421 HTPPHVINTNEKDKDNDNQQVVGIHSNN-----TDNDQIIGGFKEEQIEKQEQKPDQELE 480

Query: 481 TEDQGH-DPPTSEPTWQDTPNLILNSQDSIHGWE---ESEAAEENYGAESDYMGTSYNWF 540
            E  G  DPP+ E  WQD   L  +S DS++GWE   +SEA EE+YGA  DY+GTSY+WF
Sbjct: 481 PEQGGEVDPPSLEGIWQDGSKLNSDSHDSMNGWEAEYQSEAGEESYGA--DYVGTSYDWF 540

Query: 541 TDISRPRSYWEDRRQSWYQQMLDSNSANDEIRQLIERKTVSNFLTSDFRERMDKLMVSRL 600
            DISRPRSYWEDRRQSWYQQMLDSNSANDEIRQLI+RKTVSNFL+SDFRERMDKLMV+RL
Sbjct: 541 ADISRPRSYWEDRRQSWYQQMLDSNSANDEIRQLIQRKTVSNFLSSDFRERMDKLMVTRL 600

Query: 601 EQRTQQQEEYNEDNEDNDGAEEEEELWCFSEGHTQPKSSSDNEEEE------------ET 660
           E++TQQ+EEYNE  ED+D    +EELWCFSEGHTQPKSS DNEEE+            E 
Sbjct: 601 ERQTQQEEEYNEVKEDDDV---DEELWCFSEGHTQPKSS-DNEEEDDDERSLITAQYHEA 660

Query: 661 SDYLDQSASPLQLASPSILSSWSYQDNEMGEDSNRAASMSSPQPFPPQFSSNNQQS--SL 720
           SDYLDQSASPLQLASPSILSSWSY   +MGEDSNR  S+SSPQP  PQFSSNN Q   SL
Sbjct: 661 SDYLDQSASPLQLASPSILSSWSY---DMGEDSNRGTSISSPQPLLPQFSSNNNQHRPSL 720

Query: 721 VSRSHHASIEMELIYDLRGHMEQLYQEMSELRKSIKCCMDMQLMLQQSIKQE-VVGGGRG 780
           VS SH+ SIEMELIYDLRGHMEQLY+EMSELRKSIKCCMDMQLMLQ SIK+   VGG + 
Sbjct: 721 VSASHNPSIEMELIYDLRGHMEQLYREMSELRKSIKCCMDMQLMLQHSIKRSHEVGGRKS 780

Query: 781 RKSKSRKPKCCIICHEMQIDSLLYRCGHMCSCMKCAKELQWRGGKCPVCGAPIDDVVRAS 795
           +K KSRKPKCCI C+ M+IDSLLYRCGHMCSCMKC KELQWRGGKCPVC API+DVV+AS
Sbjct: 781 KKDKSRKPKCCI-CYNMEIDSLLYRCGHMCSCMKCGKELQWRGGKCPVCRAPIEDVVQAS 805

BLAST of MC01g1673 vs. ExPASy TrEMBL
Match: A0A5A7U932 (Altered inheritance of mitochondria protein 44 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold120G00080 PE=4 SV=1)

HSP 1 Score: 934 bits (2414), Expect = 0.0
Identity = 574/852 (67.37%), Postives = 641/852 (75.23%), Query Frame = 0

Query: 1   MASSQVEFSSSSSPFGCVLRDHNRRN------ANVAKFRNNLKTLVMDRLNDCISITPNP 60
           MASSQVEFSSSSSPFGCVLRDHNRR        +VA+FRNNLKTLVMDRLNDCI+ITPN 
Sbjct: 1   MASSQVEFSSSSSPFGCVLRDHNRRREPSVTATHVARFRNNLKTLVMDRLNDCITITPNQ 60

Query: 61  NPNSN-SNFRLPRTNENTANPSTPRRSSILERRPTK-PLHRTLSTNIDRQRNNHHISPQT 120
           N N   +NF + +TN +T   ST R     +   T  P  +T ST               
Sbjct: 61  NTNPVLTNFPVTKTNHDT---STKRSDDSQQHASTNDPQTQTTST--------------P 120

Query: 121 APP-PPPSPSK-ETSKLGASSLVQIWEKRLNVSSNHVGLNGNGNANA------------K 180
           APP P  SP+K +TSKLGASSLVQIWEKRLNVSSN +GLN N NANA            +
Sbjct: 121 APPIPETSPNKNQTSKLGASSLVQIWEKRLNVSSN-IGLNANANANANANANTAVCSTKQ 180

Query: 181 EPEPEQEQEQACSVEAGDFEDERYDAGPGSEDGFADWHSNRTSSSS---STQSQSSDAGE 240
           E E EQEQEQACSVEAGDFEDERYDAGPGS+DGFADWHS+RTSSSS   STQSQSSDAGE
Sbjct: 181 ETEAEQEQEQACSVEAGDFEDERYDAGPGSDDGFADWHSSRTSSSSPPSSTQSQSSDAGE 240

Query: 241 RESERVRVADIIRRLTLTAAKPPHPSLVEDNDQHPNESSS-HPTLSLSLREQVEHKCLSH 300
           RE  RVRV DIIRRLTLTAAKPPH S VED++  PNESSS HPTL    ++QVE KCLSH
Sbjct: 241 RE--RVRVVDIIRRLTLTAAKPPHSSWVEDHNDQPNESSSLHPTLIP--KDQVEPKCLSH 300

Query: 301 VLCSPRIRGRQAFADLLMQMERDRQKELDTLLDRRPVSKFPQRGRIQSLLRLKILKRGMV 360
           +LCSPRIRGRQAFADLL+Q+ERDRQ+ELD L++RR VSKFPQRGRIQSLLRLKILKRGM 
Sbjct: 301 ILCSPRIRGRQAFADLLLQIERDRQRELDVLVERRAVSKFPQRGRIQSLLRLKILKRGMA 360

Query: 361 VEDEQQQRPQFVITPRENQGFSTIMHLRERFSRVGENGLRSPIGEMLNNNNNNDDEKTQS 420
           +EDEQ+ RPQFVITPREN    TIMHLRERFS VGENG +SP GEMLN+N   DD+KTQ 
Sbjct: 361 LEDEQK-RPQFVITPRENHRSFTIMHLRERFSGVGENGAKSPRGEMLNDN---DDDKTQP 420

Query: 421 DTDT------HATCNN----DNDNHQVGASASASASACVSDSDSAIHSSSMNTVDDHPIP 480
           DT+       H    N    DNDN QV    S +     +D+D  I       ++     
Sbjct: 421 DTNANTHTPPHVINTNEKDKDNDNQQVVGIHSNN-----TDNDQIIGGFKEEQIEKQEQK 480

Query: 481 EDVKEQTEDQGH-DPPTSEPTWQDTPNLILNSQDSIHGWE---ESEAAEENYGAESDYMG 540
            D + + E  G  DPP+ E  WQD   L  +S DS++GWE   +SEA EE+YGA  DY+G
Sbjct: 481 PDQELEPEQGGEVDPPSLEGIWQDGSKLNSDSHDSMNGWEAEYQSEAGEESYGA--DYVG 540

Query: 541 TSYNWFTDISRPRSYWEDRRQSWYQQMLDSNSANDEIRQLIERKTVSNFLTSDFRERMDK 600
           TSY+WF DISRPRSYWEDRRQSWYQQMLDSNSANDEIRQLI+RKTVSNFL+SDFRERMDK
Sbjct: 541 TSYDWFADISRPRSYWEDRRQSWYQQMLDSNSANDEIRQLIQRKTVSNFLSSDFRERMDK 600

Query: 601 LMVSRLEQRTQQQEEYNEDNEDNDGAEEEEELWCFSEGHTQPKSSSDNEEEE-------- 660
           LMV+RLE++TQQ+EEYNE  ED+D    +EELWCFSEGHTQPKSS DNEEE+        
Sbjct: 601 LMVTRLERQTQQEEEYNEVKEDDDV---DEELWCFSEGHTQPKSS-DNEEEDDDDERSLI 660

Query: 661 -----ETSDYLDQSASPLQLASPSILSSWSYQDNEMGEDSNRAASMSSPQPFPPQFSSNN 720
                E SDYLDQSASPLQLASPSILSSWSY   +MGEDSNR  S+SSPQP  PQFSSNN
Sbjct: 661 TAQYHEASDYLDQSASPLQLASPSILSSWSY---DMGEDSNRGTSISSPQPLLPQFSSNN 720

Query: 721 QQS--SLVSRSHHASIEMELIYDLRGHMEQLYQEMSELRKSIKCCMDMQLMLQQSIKQE- 780
            Q   SLVS SH+ SIEMELIYDLRGHMEQLY+EMSELRKSIKCCMDMQLMLQ SIK+  
Sbjct: 721 NQHRPSLVSASHNPSIEMELIYDLRGHMEQLYREMSELRKSIKCCMDMQLMLQHSIKRSH 780

Query: 781 VVGGGRGRKSKSRKPKCCIICHEMQIDSLLYRCGHMCSCMKCAKELQWRGGKCPVCGAPI 795
            VGG + +K KSRKPKCCI C+ M+IDSLLYRCGHMCSCMKC KELQWRGGKCPVC API
Sbjct: 781 EVGGRKSKKDKSRKPKCCI-CYNMEIDSLLYRCGHMCSCMKCGKELQWRGGKCPVCRAPI 811

BLAST of MC01g1673 vs. TAIR 10
Match: AT1G30860.1 (RING/U-box superfamily protein )

HSP 1 Score: 239.2 bits (609), Expect = 1.1e-62
Identity = 255/844 (30.21%), Postives = 380/844 (45.02%), Query Frame = 0

Query: 10  SSSSPFGCVLRDHNRRNANVAKFRNNLKTLVMDRLNDCISITPNPNPNSNSNFRLPRTNE 69
           SS   FG +LRD   RN N   F+ NLK+ V + L   +S                  +E
Sbjct: 3   SSPQRFGLILRD---RNQNAGVFQKNLKSPVKEVLKSHVS------------------DE 62

Query: 70  NTANPSTPRRSSILERRPTKPLHRTLSTNIDRQRNNHHISPQTAPPPPPSPSKETSKLGA 129
           N  +       S +E    K  +R L + ++ +RN++                    L A
Sbjct: 63  NLVD-------SWIETHNNKN-NRVLESPLESKRNDN--------------------LVA 122

Query: 130 SSLVQIWEKRLNVSSNHVGLNGNGNANAKEPEPEQEQEQACSVEAGDFEDERYDAG---P 189
           SSLVQIWE RLN S+        GN+      P   Q  A S    D      D+G    
Sbjct: 123 SSLVQIWEARLNRSN-------GGNS------PSSSQSTASSSSRSDSGVSVQDSGLSES 182

Query: 190 GSEDGFADWHSNRTSSSSSTQSQSSDAGERESERVRVADIIRRLTLTAAKPPHPSLVEDN 249
            S DG ++         S +    SD+G  ES+  RVAD+IRRL+            ED 
Sbjct: 183 SSIDGDSEIADRTVEIESRSHGLVSDSG--ESKWGRVADLIRRLS-----------NEDK 242

Query: 250 DQHPNESSSHPTLSLSLREQVEHKCLSH---------VLCSPRIRGRQAFADLLMQMERD 309
            +   ++ S     L +       C S          V  SP+IRGRQAF D LMQMERD
Sbjct: 243 KRTAGDNGSGGGGGLEIIRTPRPYCTSSSSEKSNFPVVSFSPKIRGRQAFTDFLMQMERD 302

Query: 310 RQKELDTLLDRRPVSKFPQRGRIQSLLRLKILKRGMVVEDEQQQRPQFVITPRENQGFST 369
           R +ELD+L +R  VS+F QRGR+QS+LRL+ L R +V++D  +   +     R   G S 
Sbjct: 303 RHRELDSLFERNAVSRFTQRGRLQSMLRLRNLNRCLVIQDRNRSNAKTTGLNRIESG-SA 362

Query: 370 IMHLRERF--------------------------------------SRVGENG--LRSPI 429
           ++HLRE+F                                      +R+ + G  L +  
Sbjct: 363 VLHLREKFHANVVNSAAATDKRKNHQRSTEMNNKSVEETEGTTTTSTRLKDGGVTLEAFF 422

Query: 430 GEMLNNNNNNDDEKTQSDTDTHATCNNDNDNHQVGASASASASACVSDSDSAIHSSSMNT 489
            E L   N   +E T    +   +   D+  + +    +       +D  S        +
Sbjct: 423 AERLRPRNRKIEEATLCKEEESVSGTVDSQKNCLQLQETREVEETCNDGKSEKKEEENTS 482

Query: 490 VDDHPIPEDVKEQTEDQGHDPPTSEPTWQDTPNLILNSQDSIHGWEESEAA-------EE 549
             ++   E    Q +D+ +     + T ++T  L+  S +     ++ E +       +E
Sbjct: 483 PSEYLSRESRLRQNQDENNVGKYMQET-RETEGLVHESNEMDQCLDQQETSYLNRWGEQE 542

Query: 550 NYGAESDYMGTSYN--WFTDISRPRSYWEDRRQSWYQQMLDSNSANDEIRQLIERKTVSN 609
            Y  E  Y G  YN  W ++I+RPRSYWE+ R+S Y +++++ S  D+I +L+ER+TV++
Sbjct: 543 EYADEQSYYG-EYNDDWLSEIARPRSYWEELRKSRYLEVMNTRSEKDDICRLLERRTVTD 602

Query: 610 FLTSDFRERMDKLMVSRLEQRTQQQEEYNEDNEDNDGAEEEEELWCFSEGHTQPKSSSDN 669
           FL S  RE++D LM+SR++  + +  +  E  +     EEEEE+    +   + +     
Sbjct: 603 FLDSGLREKIDNLMMSRVQTHSDKHSKKWELQQ-----EEEEEVNFEIDEEIKEEPLRGG 662

Query: 670 EEEEETSDYLDQSASPLQLASPSILSSWSYQDNEMGEDSNRAASMSSPQPFPPQFSSNNQ 729
           EE+++  D    S+S +  +SP+   SWS QD ++   S  A  + +PQ           
Sbjct: 663 EEQDDRDDLSQSSSSHISASSPA--GSWSSQDTDV--TSTPALVVQNPQ----------- 722

Query: 730 QSSLVSRSHHASIEMELIYDLRGHMEQLYQEMSELRKSIKCCMDMQLMLQQSIKQEVVGG 789
                      S EMELI  +R  ++QL QEMS LR S+K C+D    LQ    QE    
Sbjct: 723 -----------SPEMELISGMRSQIQQLQQEMSVLRDSVKTCLDANASLQHKAHQE---- 726

Query: 790 GRGRKSKSRKPKCCIICHEMQIDSLLYRCGHMCSCMKCAKELQWRGGKCPVCGAPIDDVV 793
                    K KCC +C E Q++++LYRCGHMC C+KCA EL W GGKCP+C A I DVV
Sbjct: 783 ------NPMKRKCC-VCDETQVEAVLYRCGHMCMCLKCANELHWSGGKCPICRAQIVDVV 726

BLAST of MC01g1673 vs. TAIR 10
Match: AT2G34920.1 (RING/U-box superfamily protein )

HSP 1 Score: 231.9 bits (590), Expect = 1.8e-60
Identity = 247/804 (30.72%), Postives = 369/804 (45.90%), Query Frame = 0

Query: 15  FGCVLRDHNRRNANVAKFRNNLKTLVMDRLNDCISITPNPNPNSNSNFRLPRTNENTANP 74
           FG VLRD N+R+ +   F+ NLK  V       IS   + N   +S     R   N + P
Sbjct: 8   FGSVLRDRNQRHNDDVVFKKNLKAQV-KTAPPAISDESSEN-RVDSLIGNKRKKNNKSRP 67

Query: 75  STPRRSSILERRPTKPLHRTLSTNIDRQRNNHHISPQTAPPPPPSPSKETSKLGASSLVQ 134
            +P +                     R R  ++ S                  GASSLVQ
Sbjct: 68  GSPEK--------------------PRTRKGNNFSDNLG--------------GASSLVQ 127

Query: 135 IWEKRLNVSSNHVGLNGNGNANAKEPEPEQEQEQACSVEAGDFEDERYDAGPGSEDGFAD 194
           IWE RLN S+      GN   +++  E   E     SV+      E +   P S DG ++
Sbjct: 128 IWEARLNRSN-----GGNSAIHSQSIEISSE----ASVQ------EIHLLAP-SIDGESE 187

Query: 195 WHSNRTSSSSSTQSQSSDAGERESERVRVADIIRRLTLTAAKPPHPSLVEDNDQHPNESS 254
              +   S S  Q+   ++G   S    V+DIIRRL+              N+Q    S+
Sbjct: 188 ---SENESKSPDQTVEIESGTLNS----VSDIIRRLS--------------NEQKLTASN 247

Query: 255 SHPTLSLSL--REQVEHKCLSHVLCSPRIRGRQAFADLLMQMERDRQKELDTLLDRRPVS 314
           +   + + +     +E      V CSPRIRGRQA++DLL+ +ER+R +EL++LL R  VS
Sbjct: 248 NGGAVDMPIVKTPTLEKSSFQVVTCSPRIRGRQAYSDLLVHLERERHRELESLLGRNAVS 307

Query: 315 KFPQRGRIQSLLRLKILKRGMVVEDEQQQRPQFVITPRENQGFSTIMHLRERFSRVGENG 374
           +FPQRGR+QS+LRL+ LKRG+ ++D  +   +  +     Q  STI+HLRE+      N 
Sbjct: 308 RFPQRGRLQSMLRLRSLKRGLAIQDRHRGTTKSDL--NRFQPSSTILHLREKLREKAANA 367

Query: 375 ---------------------------LRSPIGEMLNNNNNNDDEKTQSDTDTHATCNND 434
                                      L SP  E L+    N +E      +T    +  
Sbjct: 368 AAEAGLKKGQQSTVETESMQSKETSGILHSPSTERLSPQKRNIEEAILRKNETETKMS-- 427

Query: 435 NDNHQVGASASASASACVSDSDSAIHSSSMNTVDDHPIPEDVKEQTEDQGHDPPTSEPTW 494
                +    +  A     +SD+    +S+   +   +      + E+ G     +E T 
Sbjct: 428 ----YLQLKKAIVAEVLERESDNTSPLTSVTPQEPRIL------RNEEAGKLESGTEGT- 487

Query: 495 QDTPNLILNSQDSIHGWEESEAAEENYGAESDYMGTSYNWFTDISRPRSYWEDRRQSWYQ 554
           Q+TP L         GWEE E  E+    +S Y   SY+WFT+ISRPR+YWED R+S Y 
Sbjct: 488 QETPFLETQEMSFQSGWEEQEEYEDE---QSYYGDMSYDWFTEISRPRTYWEDLRKSRYL 547

Query: 555 QMLDSNSANDEIRQLIERKTVSNFLTSDFRERMDKLMVSRLEQRTQQQEEYNEDNEDNDG 614
           +++++ S  D+I +L+ER+TVS FL S  RE++DKL++SR++     + E        + 
Sbjct: 548 EVMNTKSDKDDICRLLERRTVSGFLQSGLREKIDKLIMSRVQIHPAHRIE--------EA 607

Query: 615 AEEEEELWCFSEGHTQPKSSSDNEEEEETSDYLDQSASPLQLASPSILSSWSYQDNEMGE 674
            +EEE+                 EE++E  D L QS+S  Q+ +PS   SWS  D  +  
Sbjct: 608 TKEEEKY-------------DIGEEKDEDRDDLSQSSS--QIFAPSPAGSWSSLDTGV-- 645

Query: 675 DSNRAASMSSPQPFPPQFSSNNQQSSLVSRSHHASIEMELIYDLRGHMEQLYQEMSELRK 734
                                   +S  + + H+++++               EMSELR 
Sbjct: 668 ------------------------TSTPTHNLHSTLQL---------------EMSELRD 645

Query: 735 SIKCCMDMQLMLQQSIKQEVVGGGRGRKSKSRKPKCCIICHEMQIDSLLYRCGHMCSCMK 790
           S+K C+D+   LQ+S+  E             K KCC +C+E Q+++LLYRCGHMC+C++
Sbjct: 728 SVKTCLDVNASLQKSVHLE----------NPFKRKCC-VCNETQVETLLYRCGHMCTCLR 645

BLAST of MC01g1673 vs. TAIR 10
Match: AT5G04460.1 (RING/U-box superfamily protein )

HSP 1 Score: 145.6 bits (366), Expect = 1.7e-34
Identity = 196/696 (28.16%), Postives = 305/696 (43.82%), Query Frame = 0

Query: 152 NGNANAKEPEPEQEQEQACSVEAGDFEDERYDAGPGSEDGFADWHSNRTSSSSSTQSQSS 211
           N N +++E  P          + GD E ER             W  +R +  SS   Q  
Sbjct: 230 NNNTSSREQSP----------DLGDGERERV------RHIARGWMDSRINDHSSNVRQRD 289

Query: 212 D------AGERESERVRVADIIRRLTLTAAKPPHPSLVEDNDQHPNESSSHPTLSLSLRE 271
           D       G+ E ERVR+     ++T         +  ED         +H       R 
Sbjct: 290 DNRRPEWLGDTERERVRIIREWMQMTSQQRGGARATPREDQRSTSEADRNHDAAPQVDRV 349

Query: 272 QVEHKCLSHVLCSP--------RIRGRQAFADLLMQMERDRQKELDTLLDRRPVSKFPQR 331
           +V    ++H    P        R+RGRQA  DLLM+ ER+RQ+EL  LL+ R VS F  R
Sbjct: 350 RV-GLAVNHEEGQPPHVRRDLRRVRGRQALLDLLMRAERERQRELQGLLEHRAVSDFAHR 409

Query: 332 GRIQSLLRLKILK--RGMVVEDEQQQRPQFVITPRENQGFSTIMHLRERFSRVGENGLRS 391
            RIQSLLR + L+  R  V E       + ++  RE Q   T+  LRE F     NG  +
Sbjct: 410 NRIQSLLRGRFLRNERPTVPERTPSMASRELLQLRERQ---TVSGLREGF----HNGREN 469

Query: 392 PIGEMLNNNNNNDDEKTQSDTDTHATCNNDNDNHQVGASASASASACVSDSDSAIHSSSM 451
            + E   N +N D++ + + T+  A      ++ +V    S+++S   +DS     +S  
Sbjct: 470 IVHE---NTSNTDNDNSNTSTNALAIAITAGNSQRV-TDESSTSSRQGNDSPILPDNSES 529

Query: 452 NTVD-DHPIPEDVKEQTEDQGHDPPTSEPTWQDT--------PNLILNSQDS-------- 511
           N  + D    ED  ++   Q + P    P  + T         N  +N  ++        
Sbjct: 530 NLANADRDWEEDTNQRRIWQENVPVDERPNLEQTTLTQFDGYDNTDINRDETSVSDMHRE 589

Query: 512 IHGWEESE-AAEENYGA--ESDYMGTSYNW---FTDISRPRSYWEDRRQSWYQQMLDSNS 571
             G+ + E   +E +G   E+    +  NW    ++  R R   + RR + +    D N 
Sbjct: 590 ASGFADDEYRTQEAHGVWHENSSRQSDGNWPGTRSEALRSRRVVQLRRLNRFHPPEDDNV 649

Query: 572 ANDEIRQLIERKTVSNFLTSDFRERMDKLMVSRLEQRTQQQEEYNEDNEDNDGAEEEEEL 631
            + E+R+L+ R++VSN L S FRE +D+L+ S  E+R     ++                
Sbjct: 650 YSMELRELLSRRSVSNLLHSGFRESLDQLIQSYAERRGHTHVDW---------------- 709

Query: 632 WCFSEGHTQPKSSSDNEEEEETSDYLDQSASPLQLASPSILSSWSYQDNEMGEDSNRAAS 691
               + H   +++  +  E +T   +                 +   DN++   +     
Sbjct: 710 ----DLHANLQTAIPDSPERDTDHQV-----------------FVRNDNQLNGINGSQLL 769

Query: 692 MSSPQPFPPQFSSNNQQSSLVSR--SHHASIEMELIYDLRGHMEQLYQEMSELRKSIKCC 751
            + P P P      +   +  SR   H + IE E++ DLRG + +L Q MS++++ ++ C
Sbjct: 770 PTPPAPPPQPIWHQDLHHTSWSRHSMHRSEIEWEVMNDLRGDVARLQQGMSQMQRMLEAC 829

Query: 752 MDMQLMLQQSIKQEV------VGGGRG----------RKSKSRKPKCCIICHEMQIDSLL 791
           MDMQL LQ+S++QEV        G +G          R S   K  CC+ C +  ID+LL
Sbjct: 830 MDMQLELQRSVRQEVSAALNRSAGDQGMSAETSEDGSRWSHVSKGTCCVCC-DNHIDALL 859

BLAST of MC01g1673 vs. TAIR 10
Match: AT5G04460.3 (RING/U-box superfamily protein )

HSP 1 Score: 145.6 bits (366), Expect = 1.7e-34
Identity = 196/696 (28.16%), Postives = 305/696 (43.82%), Query Frame = 0

Query: 152 NGNANAKEPEPEQEQEQACSVEAGDFEDERYDAGPGSEDGFADWHSNRTSSSSSTQSQSS 211
           N N +++E  P          + GD E ER             W  +R +  SS   Q  
Sbjct: 230 NNNTSSREQSP----------DLGDGERERV------RHIARGWMDSRINDHSSNVRQRD 289

Query: 212 D------AGERESERVRVADIIRRLTLTAAKPPHPSLVEDNDQHPNESSSHPTLSLSLRE 271
           D       G+ E ERVR+     ++T         +  ED         +H       R 
Sbjct: 290 DNRRPEWLGDTERERVRIIREWMQMTSQQRGGARATPREDQRSTSEADRNHDAAPQVDRV 349

Query: 272 QVEHKCLSHVLCSP--------RIRGRQAFADLLMQMERDRQKELDTLLDRRPVSKFPQR 331
           +V    ++H    P        R+RGRQA  DLLM+ ER+RQ+EL  LL+ R VS F  R
Sbjct: 350 RV-GLAVNHEEGQPPHVRRDLRRVRGRQALLDLLMRAERERQRELQGLLEHRAVSDFAHR 409

Query: 332 GRIQSLLRLKILK--RGMVVEDEQQQRPQFVITPRENQGFSTIMHLRERFSRVGENGLRS 391
            RIQSLLR + L+  R  V E       + ++  RE Q   T+  LRE F     NG  +
Sbjct: 410 NRIQSLLRGRFLRNERPTVPERTPSMASRELLQLRERQ---TVSGLREGF----HNGREN 469

Query: 392 PIGEMLNNNNNNDDEKTQSDTDTHATCNNDNDNHQVGASASASASACVSDSDSAIHSSSM 451
            + E   N +N D++ + + T+  A      ++ +V    S+++S   +DS     +S  
Sbjct: 470 IVHE---NTSNTDNDNSNTSTNALAIAITAGNSQRV-TDESSTSSRQGNDSPILPDNSES 529

Query: 452 NTVD-DHPIPEDVKEQTEDQGHDPPTSEPTWQDT--------PNLILNSQDS-------- 511
           N  + D    ED  ++   Q + P    P  + T         N  +N  ++        
Sbjct: 530 NLANADRDWEEDTNQRRIWQENVPVDERPNLEQTTLTQFDGYDNTDINRDETSVSDMHRE 589

Query: 512 IHGWEESE-AAEENYGA--ESDYMGTSYNW---FTDISRPRSYWEDRRQSWYQQMLDSNS 571
             G+ + E   +E +G   E+    +  NW    ++  R R   + RR + +    D N 
Sbjct: 590 ASGFADDEYRTQEAHGVWHENSSRQSDGNWPGTRSEALRSRRVVQLRRLNRFHPPEDDNV 649

Query: 572 ANDEIRQLIERKTVSNFLTSDFRERMDKLMVSRLEQRTQQQEEYNEDNEDNDGAEEEEEL 631
            + E+R+L+ R++VSN L S FRE +D+L+ S  E+R     ++                
Sbjct: 650 YSMELRELLSRRSVSNLLHSGFRESLDQLIQSYAERRGHTHVDW---------------- 709

Query: 632 WCFSEGHTQPKSSSDNEEEEETSDYLDQSASPLQLASPSILSSWSYQDNEMGEDSNRAAS 691
               + H   +++  +  E +T   +                 +   DN++   +     
Sbjct: 710 ----DLHANLQTAIPDSPERDTDHQV-----------------FVRNDNQLNGINGSQLL 769

Query: 692 MSSPQPFPPQFSSNNQQSSLVSR--SHHASIEMELIYDLRGHMEQLYQEMSELRKSIKCC 751
            + P P P      +   +  SR   H + IE E++ DLRG + +L Q MS++++ ++ C
Sbjct: 770 PTPPAPPPQPIWHQDLHHTSWSRHSMHRSEIEWEVMNDLRGDVARLQQGMSQMQRMLEAC 829

Query: 752 MDMQLMLQQSIKQEV------VGGGRG----------RKSKSRKPKCCIICHEMQIDSLL 791
           MDMQL LQ+S++QEV        G +G          R S   K  CC+ C +  ID+LL
Sbjct: 830 MDMQLELQRSVRQEVSAALNRSAGDQGMSAETSEDGSRWSHVSKGTCCVCC-DNHIDALL 859

BLAST of MC01g1673 vs. TAIR 10
Match: AT4G20160.1 (LOCATED IN: chloroplast; EXPRESSED IN: 6 plant structures; EXPRESSED DURING: L mature pollen stage, M germinated pollen stage, 4 anthesis, petal differentiation and expansion stage; BEST Arabidopsis thaliana protein match is: RING/U-box superfamily protein (TAIR:AT1G30860.1); Has 64917 Blast hits to 39974 proteins in 1949 species: Archae - 279; Bacteria - 7425; Metazoa - 26604; Fungi - 5788; Plants - 3193; Viruses - 275; Other Eukaryotes - 21353 (source: NCBI BLink). )

HSP 1 Score: 142.1 bits (357), Expect = 1.9e-33
Identity = 124/370 (33.51%), Postives = 187/370 (50.54%), Query Frame = 0

Query: 403  NNDNDNHQVGASASASASACVSDSDSAIHSSSMNTVDDHPIPEDVK--EQTEDQGHDPPT 462
            N   +  Q   SAS  +     + +S+      +T   H  P D K  ++ ED+      
Sbjct: 839  NKSQEVSQGEESASHGSRESAKEKNSSQQDDETST---HRNPNDKKGIKEPEDEESKKVE 898

Query: 463  SEPTWQDTPNLILNSQDSIHGWEESEAAEENYGAESDYMGTSYNWFTDISRPRSYWEDRR 522
             E T +   N+   S + ++ W+ +E  EE      DY     +W  DISRPRSYWED R
Sbjct: 899  REETGE---NVEEASVEFVNDWDGNEMEEEEEEEYGDYFNGDDDWIHDISRPRSYWEDLR 958

Query: 523  QSWYQQMLDSNSANDEIRQLIERKTVSNFLTSDFRERMDKLMVSRLEQRTQQQEEYNEDN 582
            +  Y ++L++ S   +I  LIER+TVSNFLTSD R+++D LM++R++            N
Sbjct: 959  KERYLEVLNTESEKKDICNLIERRTVSNFLTSDLRQKIDNLMITRVQSHIGVPL-----N 1018

Query: 583  EDNDGAEEEEELWCFSEGHTQPKSSSDNEEEEE--------TSDYLDQSASPLQLASPSI 642
            +  +G E EEE W   E     ++  DNE EEE         SD   QS+     A  S 
Sbjct: 1019 QIEEGDEYEEE-W---EVECSARNEEDNETEEEPEKTNLEAPSDVCSQSS-----ARSST 1078

Query: 643  LSSWSYQDNEMGEDSNRAASMSSPQPFPPQFSSNNQQSSLVSRSHHASIEMELIYDLRGH 702
            + SW+++D ++ +D+    S+S P+P  P              ++ ++ +M+ I DL+  
Sbjct: 1079 MMSWNFRDQDIDKDNEPTTSLSLPEPLVP--------------TNQSTQDMQTISDLKEQ 1138

Query: 703  MEQLYQEMSELRKSIKCCMDMQLMLQQSIKQEVVGGGRGR------KSKSRKPKCCIICH 757
            MEQL +EM ELR ++K C+DMQL  Q+S+ Q++   G         K    K KCC +C 
Sbjct: 1139 MEQLQREMLELRNTVKSCIDMQLHFQKSVTQDLSRSGSSAEQRVDPKKDPLKRKCC-VCS 1173

HSP 2 Score: 102.1 bits (253), Expect = 2.2e-21
Identity = 120/408 (29.41%), Postives = 181/408 (44.36%), Query Frame = 0

Query: 1   MASSQVEFSSSSSPFGCVL---------RDHNR-------------RNANVAKFRNNLKT 60
           MASS+VE SS  + F CVL         RD+N              RN +VA F  NL  
Sbjct: 1   MASSEVEMSSCQT-FNCVLSRPHERCTQRDNNTNNNKKKNNNNNNVRNIHVAAFEKNLNV 60

Query: 61  LVMDRLNDC-ISITPNPNPNSNSNFRLPRTNENTANPSTPRRSSILERRPTKPLHRTLST 120
            V D L +C +S+    +   +S   +P  + N ++ S  R S   E R   P   + S+
Sbjct: 61  FVRDHLENCSVSV---DDVVDDSIKAVPECSSNKSSVSDHRLSKTEESRQDVP---SSSS 120

Query: 121 NIDRQRNNHHISPQTAPPPPPSPSKETSKLGASSLVQIWEKR------------LNVSSN 180
           ++    +     P +  P  P      S+  ASSLVQIWE R            ++  ++
Sbjct: 121 SLGSDSD-----PNSGQPENP------SRNAASSLVQIWEARTTQQPPSSNQSLIDSRTS 180

Query: 181 HVGLNGNGNANAKEPEPEQEQEQACSVEAGDFEDERYDAGPGSEDGFADWHSNRTSSSSS 240
            +G N   N+ + E   E +  Q       + E+E  +  P                   
Sbjct: 181 SMGSNALENSESLESVKESQMIQPIEECNNEEEEEEIECAP------------------- 240

Query: 241 TQSQSSDAGERESERVRVADIIRRLTLTAAKPPHPSLVEDNDQHPNESSSHPTLSLSLRE 300
                 ++GE++ E VRV DIIR+L+  +    +     DN    N++S          +
Sbjct: 241 ---PPLESGEKDREGVRVMDIIRKLSNDSETITN----NDNGSSGNDNSKEV-------Q 300

Query: 301 QVEHKCLSHVLCSPRIRGRQAFADLLMQMERDRQKELDTLLDRRPVSKFPQRGRIQSLLR 360
             E +    V CSPRIRGRQA ADLL+QM RDR+K+L  L +R  VSKF  RGRIQS LR
Sbjct: 301 TTEARSFPQVACSPRIRGRQALADLLVQMTRDREKDLACLRERHCVSKFTNRGRIQSTLR 357

Query: 361 LKILKRGMVVEDEQQQRPQFV--ITPRENQGFSTIMHLRERFSRVGEN 372
           ++  +R + ++   + +       + R ++G   +  LRE++    EN
Sbjct: 361 IRCYERCLAIQGRHRSKSTSAGSDSNRSSRGSGVMHLLREKYKANSEN 357