MC01g1612 (gene) Bitter gourd (Dali-11) v1

BLAST of MC01g1612 vs. ExPASy Swiss-Prot
Match: Q5BQN5 (WPP domain-associated protein (Fragment) OS=Solanum lycopersicum OX=4081 GN=WAP PE=1 SV=1)

HSP 1 Score: 516.5 bits (1329), Expect = 5.7e-145
Identity = 339/844 (40.17%), Postives = 523/844 (61.97%), Query Frame = 0

Query: 23  EDLGDDIQILEDLDSYLEDIDDRLTISRMVSDAVIKGMVNAISEEANEKITRKESEVSEL 82
           E+LGD  +ILED ++Y ED++DRL +SRMVSD+VIKG+V+A+ +EA E++  K+ E++ L
Sbjct: 3   ENLGD--EILEDFETYWEDVNDRLMVSRMVSDSVIKGIVSAVEQEAAERLVTKDMELANL 62

Query: 83  REILKCYHLGSEKN--KSLVSPLRSNKSKSTKCDRNEGISGALFEHDEMRDSVCNLKNAA 142
           +E L+ +  G  K   +S  S +  N+ +S    +   +S    EH +M + +  L++ A
Sbjct: 63  KEYLQFHEGGLSKTELESFGSLMSQNELESMDFRKCMTLSDVFMEHGKMGEFLDGLRSLA 122

Query: 143 KENFKKLRKEIDKIRGCSSIRKINSGSELVGLSGILQEKASSRCVDVDKIVDDLQDNLDT 202
           K+ FKKL+K ID++RG +S+    S SE+  L GILQEK S   V +DK +D+++  +DT
Sbjct: 123 KDEFKKLKKSIDELRGSNSVSNKISRSEMAKLEGILQEKESGIWVQLDKTLDNIRMMVDT 182

Query: 203 FYNQVEDIVQVSKASLGQWQDEKEFLADIEGMVIRNYIWSVQQELEEKLWDQNAKIFSNE 262
            + +++ ++Q+SK SL  WQ+E     ++E MV++  I +VQ+E E KLWDQ A++  + 
Sbjct: 183 VFKRMDVMLQLSKTSLHHWQEEHLIKVELESMVMQCVIRTVQEEFEYKLWDQYAQLCGDR 242

Query: 263 RKIWVQKIKEISSLRQELDIISKSL-SPEVGHLFSYSSMDSDHSHRKLLSNHMAPS---- 322
                +K+  ISSLR ELD + KSL S E GH+ S+ S D+D   RK  S ++  +    
Sbjct: 243 N----EKLNAISSLRTELDAVLKSLSSSENGHVTSHGSHDADFFTRKKSSEYVTSTKSVW 302

Query: 323 TGNGKHELSKTNLPENVDPARLKHLNRDELINHFNTEMTKMSRNHESLVHEITEENFTLK 382
            GNGK E SKT++PEN D   LKH+++DE++ +FN  MTKM R+HES++ + T+E F L+
Sbjct: 303 DGNGKLEDSKTDIPENFDAVTLKHMSKDEMVTYFNNIMTKMKRHHESILQKKTDEYFVLR 362

Query: 383 RELLKEKEKSSL--KKDK-EFDILRKKIPDIILKLDDILMENEKLSVSAANDENLGTMRN 442
            E L  +  S +  KKDK E DILRKKIP+II KLDDIL+ENEK         + G +++
Sbjct: 363 AEYLNLRGGSVVPHKKDKGESDILRKKIPEIIFKLDDILVENEKHPAFTQETLSFGNLKD 422

Query: 443 RLESLIAENRHLKDSLEEKKKEVKCLSLQISSSSEKMSQQSLRHAKSLNTIEKIKCEMQD 502
           RL++L++EN  L+D ++EKK EVK L  Q+S ++EK  Q SL  A  L  I ++   M++
Sbjct: 423 RLDNLLSENHQLRDLVKEKKNEVKSLLSQVSDATEKRLQHSLAEAGMLKQIGELNLAMEE 482

Query: 503 AKFEASIGEDIVKCFLRETMDTIRCVNEESALRYDIMQGIYETIFQGASFRGELASTSED 562
           +    S+ ED+  CFLR+     R   EE  L ++++    +T   G++ + E+      
Sbjct: 483 SLIGGSVREDVYTCFLRDLSGGARNEVEELNLGFNMINESNDT-SAGSTRKIEI------ 542

Query: 563 EHLDEESIIMQGILGVVLQESWKEAEEKMTSLNNRYIEEMRIRQSLEKEVLHCGEALRVE 622
           E L+ E +IMQ I GV+  E  KEA++ +  L   ++ E  IR SL+ +++     L+ E
Sbjct: 543 EDLEMECLIMQEICGVISGEGIKEAKDMLKELYLEHLNEKEIRTSLDTKLIEMENKLKFE 602

Query: 623 IFEKQKLQAELVLLGASLKEKEQLVQEITSALEKEKGKLALAYEEVGSLKDQTNRQETLI 682
           + EK +    L+ +   + EKE+L  + ++AL KE+ +     +E+ + K+  ++Q+TL 
Sbjct: 603 VEEKDR----LMQMEKLVNEKEKLATDASAALAKERVQSEQVRQELNAAKEFASQQQTLA 662

Query: 683 LKGHEESNITQLKLADALRKVEFLEVETCKSKQNLEQAMMELSKVDEERRMLVARLLESQ 742
              ++E N+ + +LA+A+ ++E L+ E  +   +LE+   EL + +    M++A   E Q
Sbjct: 663 SGCNKEVNVIKGQLAEAVERIEVLKEEVAQLNISLEEKTEELKEANHRANMVLAISEERQ 722

Query: 743 KTELLVEEKEKESRKQMESVIILVQELLKEVFDFEHRAIDYISRNNKRLESLSSQTNSLI 802
                +E KE   RKQ+E +I  + E  K + DFE R    +  NN R E   SQ + L+
Sbjct: 723 TLLSSLESKEIALRKQVEKIIGNINESSKMIADFECRVTGRLKTNNARFEHSFSQMDCLV 782

Query: 803 QNACMLKRAGLIYKQRHEKRCSDLQKAEAEVDLLGDEVDALLRLLEKMYIALDHYSPILQ 857
           + A +L+R  L+Y+QR EKRCSDL+ AEAEVDLLGDEVD LL L+EK+YIALDHYSP+LQ
Sbjct: 783 KKANLLRRTTLLYQQRLEKRCSDLKLAEAEVDLLGDEVDTLLSLVEKIYIALDHYSPVLQ 829

BLAST of MC01g1612 vs. ExPASy Swiss-Prot
Match: O64584 (WPP domain-associated protein OS=Arabidopsis thaliana OX=3702 GN=WAP PE=1 SV=2)

HSP 1 Score: 433.7 bits (1114), Expect = 4.9e-120
Identity = 324/893 (36.28%), Postives = 489/893 (54.76%), Query Frame = 0

Query: 1   MDANATPEENGNMQ---------LSASDKQLEDLGDDIQILEDLDSYLEDIDDRLTISRM 60
           M+      ENG+++         L  +    E+   D+  LEDLDSY EDI+DRLTISR+
Sbjct: 1   MEEEVVKSENGSLEFHDDTLSSSLQVNGVLKENENPDVDFLEDLDSYWEDINDRLTISRV 60

Query: 61  VSDAVIKGMVNAISEEANEKITRKESEVSELREILKCYHLGSEKNKSLVSPLRSNKSKST 120
           VSD++I+GMV AI  +A EKI +K+ E+S++RE L  YH+GSE+N+S  S L        
Sbjct: 61  VSDSIIRGMVTAIESDAAEKIAQKDLELSKIRETLLLYHVGSEENESSESRL-------- 120

Query: 121 KCDRNEGISGALFEHDEM-RDSVCNLKNAAKENFKKLRKEIDKIRGCSSIRKINSGSELV 180
                         HDE+ + S  +LK  A++    L +E+  +R    I    SG+ + 
Sbjct: 121 -------------IHDELTQGSSSSLKKKARKQLLMLVEELTNLREYIHIN--GSGATVD 180

Query: 181 GLSGILQEKASSRCVDVDKIVDDLQDNLDTFYNQVEDIVQVSKASLGQWQDEKEFLADIE 240
              G+      +R   VDK++D L+  L+T   +  D+   S      WQ E +F  +IE
Sbjct: 181 DSLGLDSSPHETRSKTVDKMLDSLKSILETVLKRKNDMELPS-----SWQQEHDFQKEIE 240

Query: 241 GMVIRNYIWSVQQELEEKLWDQNAKIFSNERKIWVQKIKEISSLRQELDIISKSL----- 300
             V+ + + S++ E E++L DQ A+ F   R + +  IKEI+ LRQEL+ I KS      
Sbjct: 241 SAVVTSVLRSLKDEYEQRLLDQKAE-FGGNRSLILGNIKEITGLRQELEAIRKSFLDHEN 300

Query: 301 SPEVGHLFSYSSMDSDHSHRKLLSNHMAPSTGNGKHELSKTNL-PENVDPARLKHLNRDE 360
             E G +     ++  H       N ++    NGKHE S T L PE+ +   L+H++ DE
Sbjct: 301 GDEAGEVGDRKRVEQLHRKMSGSLNSVSSVWENGKHEESSTGLIPEHNE--TLRHMSPDE 360

Query: 361 LINHFNTEMTKMSRNHESLVHEITEENFTLKRELLKEKEKSSLK---KDKEFDILRKKIP 420
           +INHF  EM KM R+H+  + E+TE+ FT KR+ L   E+ S     KDKE   L+KKIP
Sbjct: 361 MINHFKIEMNKMKRDHDYKIQELTEQCFTFKRKYLNLTERGSFSFVGKDKELGALKKKIP 420

Query: 421 DIILKLDDILMENEKLSVSAANDENLGTMRNRLESLIAENRHLKDSLEEKKKEVKCLSLQ 480
            +I KLD ILME+EK      ND  L   + +L+SL+ ENR LKDSL             
Sbjct: 421 FVISKLDKILMEDEKFVSEGKNDAGL---KRQLDSLLLENRQLKDSL------------- 480

Query: 481 ISSSSEKMSQQSLRHAKSLNTIEKIKCEMQDAKFEASIGEDIVKCFLRETMDTIRCVNEE 540
            S ++EKMSQ S   A     I K++ +++D++ EASI ED+  CF+ E +  I+C  +E
Sbjct: 481 -SDAAEKMSQLSQAEADHQELIRKLETDVEDSRNEASIYEDVYGCFVTEFVGQIKCTKQE 540

Query: 541 SALRYDIMQGIYETIFQGASFRGELASTSEDEHLDEESIIMQGILGVVLQESWKEAEEKM 600
           + L + +++  YE + +  + +    S  + E    +S++M+    V+ +E+ KEA +K+
Sbjct: 541 TDLEHSMLREAYELLLEDLARKEARKSKEDFEDSCVKSVMMEECCSVIYKEAVKEAHKKI 600

Query: 601 TSLNNRYIEEMRIRQSLEKEVLHCGEALRVEIFEKQKLQAELVLLGASLKEKEQLVQEIT 660
             LN    E+                 LR E+ +K++L+ E+  LG  +KEKE LVQ   
Sbjct: 601 VELNLHVTEK--------------EGTLRSEMVDKERLKEEIHRLGCLVKEKENLVQTAE 660

Query: 661 SALEKEKGKLALAYEEVGSLKDQTNRQETLILKGHEESNITQLKLADALRKVEFLEVETC 720
           + L  E+ K+ +  +++  L+ Q  RQET I    E  ++     A  L KV+  E +  
Sbjct: 661 NNLATERKKIEVVSQQINDLQSQVERQETEIQDKIEALSVVS---ARELEKVKGYETKIS 720

Query: 721 KSKQNLEQAMMELSKVDEERRMLVARLLESQKTELLVEEKEKES-RKQMESVIILVQELL 780
             ++ LE A   L ++ +E+R    +L E+        + EKE+ +KQ+ S+ ++V   L
Sbjct: 721 SLREELELARESLKEMKDEKRKTEEKLSET--------KAEKETLKKQLVSLDLVVPPQL 780

Query: 781 KEVFD-FEHRAIDYISRNNKRLESLSSQTNSLIQNACMLKRAGLIYKQRHEKRCSDLQKA 840
            + FD  E    +   + N RL+++ SQ + L      +K     YKQR EK+C DL+KA
Sbjct: 781 IKGFDILEGLIAEKTQKTNSRLKNMQSQLSDLSHQINEVKGKASTYKQRLEKKCCDLKKA 820

Query: 841 EAEVDLLGDEVDALLRLLEKMYIALDHYSPILQHYPGIVDILKLVKRELSGDT 873
           EAEVDLLGDEV+ LL LLEK+YIALDHYSPIL+HYPGI++IL+LV+RELSG++
Sbjct: 841 EAEVDLLGDEVETLLDLLEKIYIALDHYSPILKHYPGIIEILRLVRRELSGES 820

BLAST of MC01g1612 vs. NCBI nr
Match: XP_022146598.1 (WPP domain-associated protein [Momordica charantia] >XP_022146599.1 WPP domain-associated protein [Momordica charantia])

HSP 1 Score: 1624 bits (4205), Expect = 0.0
Identity = 873/876 (99.66%), Postives = 874/876 (99.77%), Query Frame = 0

Query: 1   MDANATPEENGNMQLSASDKQLEDLGDDIQILEDLDSYLEDIDDRLTISRMVSDAVIKGM 60
           MDANATPEENGNMQLSASDKQLEDLGDDIQILEDLDSYLEDIDDRLTISRMVSDAVIKGM
Sbjct: 1   MDANATPEENGNMQLSASDKQLEDLGDDIQILEDLDSYLEDIDDRLTISRMVSDAVIKGM 60

Query: 61  VNAISEEANEKITRKESEVSELREILKCYHLGSEKNKSLVSPLRSNKSKSTKCDRNEGIS 120
           VNAISEEANEKITRKESEVSELREILKCYHLGSEKNKSLVSPLRSNKSKSTKCDRNEGIS
Sbjct: 61  VNAISEEANEKITRKESEVSELREILKCYHLGSEKNKSLVSPLRSNKSKSTKCDRNEGIS 120

Query: 121 GALFEHDEMRDSVCNLKNAAKENFKKLRKEIDKIRGCSSIRKINSGSELVGLSGILQEKA 180
           GALFEHDEMRDSVCNLKNAAKENFKKLRKEIDKIRGCSSIRKINSGSELVGLSGILQEKA
Sbjct: 121 GALFEHDEMRDSVCNLKNAAKENFKKLRKEIDKIRGCSSIRKINSGSELVGLSGILQEKA 180

Query: 181 SSRCVDVDKIVDDLQDNLDTFYNQVEDIVQVSKASLGQWQDEKEFLADIEGMVIRNYIWS 240
           SSRCVDVDKIVDDLQDNLDTFYNQVEDIVQVSKASLGQWQDEKEFLADIEGMVIRNYIWS
Sbjct: 181 SSRCVDVDKIVDDLQDNLDTFYNQVEDIVQVSKASLGQWQDEKEFLADIEGMVIRNYIWS 240

Query: 241 VQQELEEKLWDQNAKIFSNERKIWVQKIKEISSLRQELDIISKSLSPEVGHLFSYSSMDS 300
           VQQELEEKLWDQNAKIFSNERKIWVQKIKEISSLRQELDIISKSLSPEVGHLFSYSSMDS
Sbjct: 241 VQQELEEKLWDQNAKIFSNERKIWVQKIKEISSLRQELDIISKSLSPEVGHLFSYSSMDS 300

Query: 301 DHSHRKLLSNHMAPSTGNGKHELSKTNLPENVDPARLKHLNRDELINHFNTEMTKMSRNH 360
           DHSHRKLLSNHMAPSTGNGKHE+SKTNLPENVDPARLKHLNRDELINHFNTEMTKMSRNH
Sbjct: 301 DHSHRKLLSNHMAPSTGNGKHEMSKTNLPENVDPARLKHLNRDELINHFNTEMTKMSRNH 360

Query: 361 ESLVHEITEENFTLKRELLKEKEKSSLKKDKEFDILRKKIPDIILKLDDILMENEKLSVS 420
           ESLVHEITEENFTLKRELLKEKEKSSLKKDKEFDILRKKIPDIILKLDDILMENEKLSVS
Sbjct: 361 ESLVHEITEENFTLKRELLKEKEKSSLKKDKEFDILRKKIPDIILKLDDILMENEKLSVS 420

Query: 421 AANDENLGTMRNRLESLIAENRHLKDSLEEKKKEVKCLSLQISSSSEKMSQQSLRHAKSL 480
           AANDENLGTMRNRLESLIAENRHLKDSLEEKKKEVKCLSLQISSSSEKMSQQSLRH KSL
Sbjct: 421 AANDENLGTMRNRLESLIAENRHLKDSLEEKKKEVKCLSLQISSSSEKMSQQSLRHGKSL 480

Query: 481 NTIEKIKCEMQDAKFEASIGEDIVKCFLRETMDTIRCVNEESALRYDIMQGIYETIFQGA 540
           NTIEKIKCEMQDAKFEASIGEDIVKCFLRETMDTIRCVNEESALRYDIMQGIYETIFQGA
Sbjct: 481 NTIEKIKCEMQDAKFEASIGEDIVKCFLRETMDTIRCVNEESALRYDIMQGIYETIFQGA 540

Query: 541 SFRGELASTSEDEHLDEESIIMQGILGVVLQESWKEAEEKMTSLNNRYIEEMRIRQSLEK 600
           SFRGELASTSEDEHLDEESIIMQGILGVVLQESWKEAEEKMTSLNNRYIEEMRIRQSLEK
Sbjct: 541 SFRGELASTSEDEHLDEESIIMQGILGVVLQESWKEAEEKMTSLNNRYIEEMRIRQSLEK 600

Query: 601 EVLHCGEALRVEIFEKQKLQAELVLLGASLKEKEQLVQEITSALEKEKGKLALAYEEVGS 660
           EVLHCGEALRVEIFEKQKLQAELVLLGASLKEKEQLVQEITSALEKEKGKLALAYEEVGS
Sbjct: 601 EVLHCGEALRVEIFEKQKLQAELVLLGASLKEKEQLVQEITSALEKEKGKLALAYEEVGS 660

Query: 661 LKDQTNRQETLILKGHEESNITQLKLADALRKVEFLEVETCKSKQNLEQAMMELSKVDEE 720
           LKDQTNRQETLILKGHEESNITQLKLADALRKVEFLEVETCKSKQNLEQAMMELSKVDEE
Sbjct: 661 LKDQTNRQETLILKGHEESNITQLKLADALRKVEFLEVETCKSKQNLEQAMMELSKVDEE 720

Query: 721 RRMLVARLLESQKTELLVEEKEKESRKQMESVIILVQELLKEVFDFEHRAIDYISRNNKR 780
           RRMLVARLLESQKTELLVEEKEKESRKQMESVIILVQELLKEVFDFEH AIDYISRNNKR
Sbjct: 721 RRMLVARLLESQKTELLVEEKEKESRKQMESVIILVQELLKEVFDFEHTAIDYISRNNKR 780

Query: 781 LESLSSQTNSLIQNACMLKRAGLIYKQRHEKRCSDLQKAEAEVDLLGDEVDALLRLLEKM 840
           LESLSSQTNSLIQNACMLKRAGLIYKQRHEKRCSDLQKAEAEVDLLGDEVDALLRLLEKM
Sbjct: 781 LESLSSQTNSLIQNACMLKRAGLIYKQRHEKRCSDLQKAEAEVDLLGDEVDALLRLLEKM 840

Query: 841 YIALDHYSPILQHYPGIVDILKLVKRELSGDTTAAF 876
           YIALDHYSPILQHYPGIVDILKLVKRELSGDTTAAF
Sbjct: 841 YIALDHYSPILQHYPGIVDILKLVKRELSGDTTAAF 876

BLAST of MC01g1612 vs. NCBI nr
Match: XP_022925739.1 (WPP domain-associated protein isoform X1 [Cucurbita moschata] >XP_022925740.1 WPP domain-associated protein isoform X1 [Cucurbita moschata] >XP_022925741.1 WPP domain-associated protein isoform X1 [Cucurbita moschata] >XP_022925742.1 WPP domain-associated protein isoform X1 [Cucurbita moschata] >XP_022925743.1 WPP domain-associated protein isoform X1 [Cucurbita moschata] >XP_022925744.1 WPP domain-associated protein isoform X1 [Cucurbita moschata] >XP_022925745.1 WPP domain-associated protein isoform X1 [Cucurbita moschata] >XP_022925746.1 WPP domain-associated protein isoform X1 [Cucurbita moschata])

HSP 1 Score: 1249 bits (3231), Expect = 0.0
Identity = 694/884 (78.51%), Postives = 770/884 (87.10%), Query Frame = 0

Query: 1   MDANATPEENGNMQLSASDKQLEDLGDDIQILEDLDSYLEDIDDRLTISRMVSDAVIKGM 60
           MDANAT E+NGN+QL+ SDKQ ++LGDD   LED DSY +DI+DRLTISRMVSD+VIK M
Sbjct: 1   MDANATLEDNGNIQLTGSDKQDDNLGDDF--LEDFDSYWQDINDRLTISRMVSDSVIKVM 60

Query: 61  VNAISEEANEKITRKESEVSELREILKCYHLGSEKNKSLVSPLRSNKSKSTKCDRNEGIS 120
           VNA+S+EA+EKIT+ E EVSEL+EI+  Y LGSEK K + SPLR  K KST+CDR + I 
Sbjct: 61  VNAVSQEAHEKITQNELEVSELKEIIHSYLLGSEKTKFMTSPLRLCKLKSTECDRIDSIC 120

Query: 121 GALFEHDEMRDSVCNLKNAAKENFKKLRKEIDKIRGCSSIRKINSGSELVGL--SGILQE 180
           GA FEHDEMR+S+C+L+N AKENFKKL+KEID IRGC+S RKINSGSELVGL  SGILQE
Sbjct: 121 GAFFEHDEMRESMCSLRNTAKENFKKLKKEIDGIRGCNSSRKINSGSELVGLGLSGILQE 180

Query: 181 KASSRCVDVDKIVDDLQDNLDTFYNQVEDIVQVSKASLGQWQDEKEFLADIEGMVIRNYI 240
           KASSR +DV KIVDDLQDNLDTFYNQVED+VQ+SKAS GQWQ E+E+LADIEGMVIRNYI
Sbjct: 181 KASSRYIDVGKIVDDLQDNLDTFYNQVEDLVQLSKASFGQWQVEQEYLADIEGMVIRNYI 240

Query: 241 WSVQQELEEKLWDQNAKIFSNERKIWVQKIKEISSLRQELDIISKSLSP-EVGHLFSYSS 300
           WS+QQE EEKLWDQNAKI SNERKI  +KIKEISSLRQELD ISKSL P EVGHL S SS
Sbjct: 241 WSIQQEFEEKLWDQNAKILSNERKISAEKIKEISSLRQELDKISKSLCPSEVGHLISCSS 300

Query: 301 MDSDHSHRKLLSNHMAPST----GNGKHELSKTNLPENVDPARLKHLNRDELINHFNTEM 360
           MDSDHSHRKLLS+HM PST    GN KHE+SKTNLPENVDP+RL H+ +DELINHFNT M
Sbjct: 301 MDSDHSHRKLLSSHMNPSTLHWEGNCKHEMSKTNLPENVDPSRLNHMKKDELINHFNTVM 360

Query: 361 TKMSRNHESLVHEITEENFTLKRELLKEKEKSSL-KKDKEFDILRKKIPDIILKLDDILM 420
           TKMSRNHES VHEITEENFTLKRELLK++EKSSL KKDKEFDILR+KIPDIILKLDDIL+
Sbjct: 361 TKMSRNHESQVHEITEENFTLKRELLKKREKSSLLKKDKEFDILRRKIPDIILKLDDILL 420

Query: 421 ENEKLSVSAANDENLGTMRNRLESLIAENRHLKDSLEEKKKEVKCLSLQISSSSEKMSQQ 480
           ENEKL  S+ANDE+LGTMRNRLESLI+EN HLKD L EKKKEVKCLS Q+SS +EKMSQ 
Sbjct: 421 ENEKLRSSSANDESLGTMRNRLESLISENHHLKDLLGEKKKEVKCLSSQVSSHAEKMSQH 480

Query: 481 SLRHAKSLNTIEKIKCEMQDAKFEASIGEDIVKCFLRETMDTIRCVNEESALRYDIMQGI 540
           SL  ++SL TIEK KCEMQDA+FEASI +DI KCFLRE MD IR   EESA RYDIMQG+
Sbjct: 481 SLLVSESLTTIEKNKCEMQDAQFEASICQDIFKCFLREMMDQIRYATEESAWRYDIMQGV 540

Query: 541 YETIFQGASFRGELASTSEDEHLDEESIIMQGILGVVLQESWKEAEEKMTSLNNRYIEEM 600
           YETI++GASF GELAST+E+EHLDEESI MQG+LGVVLQES KEAEEKMTSLNNRY+EEM
Sbjct: 541 YETIYEGASFVGELASTTENEHLDEESITMQGLLGVVLQESLKEAEEKMTSLNNRYMEEM 600

Query: 601 RIRQSLEKEVLHCGEALRVEIFEKQKLQAELVLLGASLKEKEQLVQEITSALEKEKGKLA 660
             R SLEKE+LH GEALRVE+FEK+ L+AEL+   ASL+EKEQLVQEIT  LEKEK KLA
Sbjct: 601 STRLSLEKEMLHSGEALRVEVFEKEMLEAELISFRASLREKEQLVQEITFVLEKEKEKLA 660

Query: 661 LAYEEVGSLKDQTNRQETLILKGHEESNITQLKLADALRKVEFLEVETCKSKQNLEQAMM 720
           LAYEEVGSLKDQTNRQE LILK HEE NIT+LKL +A+RKV  LEVETC+SKQNLEQAM 
Sbjct: 661 LAYEEVGSLKDQTNRQEILILKSHEELNITKLKLTEAMRKVGLLEVETCESKQNLEQAMA 720

Query: 721 ELSKVDEERRMLVARLLESQKTELLVEEKEKESRKQMESVIILVQELLKEVFDFEHRAID 780
           E SKVDEERRMLVA + E+QKT+LLVEEKEKESRKQMESVI +VQELLKEVFDFEHR ID
Sbjct: 721 EFSKVDEERRMLVAMVSENQKTKLLVEEKEKESRKQMESVIFVVQELLKEVFDFEHRVID 780

Query: 781 YISRNNKRLESLSSQTNSLIQNACMLKRAGLIYKQRHEKRCSDLQKAEAEVDLLGDEVDA 840
           YISRN  RLE LSS+T SLIQ A M+KR GL+YKQ+ EKRCSDLQKAEAEVDLLGDEV A
Sbjct: 781 YISRNKGRLERLSSETKSLIQKASMVKREGLLYKQKLEKRCSDLQKAEAEVDLLGDEVAA 840

Query: 841 LLRLLEKMYIALDHYSPILQHYPGIVDILKLVKRELSGDTTAAF 876
           LLRLL KM+I LD YSPIL+HYPGIV I+KLVKRELSGD T AF
Sbjct: 841 LLRLLGKMHIELDRYSPILKHYPGIVKIMKLVKRELSGDMTEAF 882

BLAST of MC01g1612 vs. NCBI nr
Match: XP_023518244.1 (WPP domain-associated protein [Cucurbita pepo subsp. pepo] >XP_023518245.1 WPP domain-associated protein [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1248 bits (3228), Expect = 0.0
Identity = 698/884 (78.96%), Postives = 771/884 (87.22%), Query Frame = 0

Query: 1   MDANATPEENGNMQLSASDKQLEDLGDDIQILEDLDSYLEDIDDRLTISRMVSDAVIKGM 60
           MDANA  EENG M+LS SDKQ EDLGDDI  LEDLDSYL+DI+DRLTISRMVSD+VIKGM
Sbjct: 1   MDANAPLEENGKMELSDSDKQDEDLGDDI--LEDLDSYLQDINDRLTISRMVSDSVIKGM 60

Query: 61  VNAISEEANEKITRKESEVSELREILKCYHLGSEKNKSLVSPLRSNKSKSTKCDRNEGIS 120
           VNAIS+EA+EKIT+KE EVSEL+EI+  YHLGSEKN+ LVSP  S K KST+ DRN+ IS
Sbjct: 61  VNAISQEAHEKITQKELEVSELKEIVHSYHLGSEKNEFLVSPPTSWKPKSTEHDRNDSIS 120

Query: 121 GALFEHDEMRDSVCNLKNAAKENFKKLRKEIDKIRGCSSIRKINSGSELVGL--SGILQE 180
            A FEHDEMR+S+C+L+N+ +ENFKKLRKEID+IRG +SIRKINSGSELVGL  SGILQE
Sbjct: 121 DAFFEHDEMRESMCSLRNSTEENFKKLRKEIDRIRGENSIRKINSGSELVGLGLSGILQE 180

Query: 181 KASSRCVDVDKIVDDLQDNLDTFYNQVEDIVQVSKASLGQWQDEKEFLADIEGMVIRNYI 240
           KASSRC+DVDKIVDDLQDNLDT+Y QVEDIVQ+SKASLGQ Q E+E+LADIEGMVIRNYI
Sbjct: 181 KASSRCIDVDKIVDDLQDNLDTYYKQVEDIVQLSKASLGQGQVEQEYLADIEGMVIRNYI 240

Query: 241 WSVQQELEEKLWDQNAKIFSNERKIWVQKIKEISSLRQELDIISKSLSP-EVGHLFSYSS 300
             VQ+E EEKLWDQNAKI SNERKI  +KIKEIS LRQELDII KSLSP E+GHL SYSS
Sbjct: 241 CCVQEEFEEKLWDQNAKILSNERKISAEKIKEISCLRQELDIILKSLSPSEIGHLISYSS 300

Query: 301 MDSDHSHRKLLSNHMAPST----GNGKHELSKTNLPENVDPARLKHLNRDELINHFNTEM 360
           MDSDHSHRKL   HM PST    GNGKHE+SKTNLP NVDPARLKHL RDELINHFN EM
Sbjct: 301 MDSDHSHRKL---HMTPSTLLWEGNGKHEMSKTNLPTNVDPARLKHLTRDELINHFNAEM 360

Query: 361 TKMSRNHESLVHEITEENFTLKRELLKEKEKSSL-KKDKEFDILRKKIPDIILKLDDILM 420
           TKMSRNHES VH+ITEENFTLKRELLKEKEKSSL KKDKEFDILR+KIPDIILKLDDI+M
Sbjct: 361 TKMSRNHESQVHQITEENFTLKRELLKEKEKSSLLKKDKEFDILRRKIPDIILKLDDIVM 420

Query: 421 ENEKLSVSAANDENLGTMRNRLESLIAENRHLKDSLEEKKKEVKCLSLQISSSSEKMSQQ 480
           ENEK+  S ANDENLGTMR RLESLI+EN HL D L EK KEVKCLS Q+SS +E+MSQ 
Sbjct: 421 ENEKIQSSDANDENLGTMRKRLESLISENHHLNDLLGEKIKEVKCLSFQVSSHAEQMSQH 480

Query: 481 SLRHAKSLNTIEKIKCEMQDAKFEASIGEDIVKCFLRETMDTIRCVNEESALRYDIMQGI 540
           SL H KSLNTIEKI CEMQDA+FEASI EDIVKCFLRE +D IR   EESALRYDIMQGI
Sbjct: 481 SLLHVKSLNTIEKITCEMQDAQFEASICEDIVKCFLREMIDQIRYTTEESALRYDIMQGI 540

Query: 541 YETIFQGASFRGELASTSEDEHLDEESIIMQGILGVVLQESWKEAEEKMTSLNNRYIEEM 600
           Y+TI QGASF GEL S+SE+EHLDEES+IMQGILGVVLQES KEAEEKM+SLN+RY+EEM
Sbjct: 541 YKTILQGASFIGELPSSSENEHLDEESVIMQGILGVVLQESLKEAEEKMSSLNSRYMEEM 600

Query: 601 RIRQSLEKEVLHCGEALRVEIFEKQKLQAELVLLGASLKEKEQLVQEITSALEKEKGKLA 660
           RIR SLE +V HCGEAL V  FEK+KL+AEL+ + +SLKEKEQ VQEIT  LEKEK K+A
Sbjct: 601 RIRLSLEMKVFHCGEALGVAAFEKEKLEAELLSMRSSLKEKEQRVQEITFELEKEKEKVA 660

Query: 661 LAYEEVGSLKDQTNRQETLILKGHEESNITQLKLADALRKVEFLEVETCKSKQNLEQAMM 720
           LAYEEV SLKDQ N QE LILK  EESN   LKL +A++KVE LEVETC+SKQNLEQAM 
Sbjct: 661 LAYEEVDSLKDQINMQEILILKSQEESNTANLKLTEAMQKVELLEVETCESKQNLEQAMA 720

Query: 721 ELSKVDEERRMLVARLLESQKTELLVEEKEKESRKQMESVIILVQELLKEVFDFEHRAID 780
           ELSKVDEERRML A + E+++T LLVEEKEKESRKQ+ESVI +VQ+LLKEVFDFEHR +D
Sbjct: 721 ELSKVDEERRMLAAMVSENKETMLLVEEKEKESRKQIESVIFVVQDLLKEVFDFEHRVVD 780

Query: 781 YISRNNKRLESLSSQTNSLIQNACMLKRAGLIYKQRHEKRCSDLQKAEAEVDLLGDEVDA 840
           YISRNN+RLESLSS+T SL+QNA MLKRA L++KQR EKRCSDLQKAEAEVDLLGDEVDA
Sbjct: 781 YISRNNERLESLSSETKSLVQNASMLKRASLLFKQRFEKRCSDLQKAEAEVDLLGDEVDA 840

Query: 841 LLRLLEKMYIALDHYSPILQHYPGIVDILKLVKRELSGDTTAAF 876
           LLRLLEKMY ALD YSPIL+HYPGIV +LKL+KRELS DTT AF
Sbjct: 841 LLRLLEKMYNALDSYSPILKHYPGIVKVLKLLKRELSRDTTEAF 879

BLAST of MC01g1612 vs. NCBI nr
Match: XP_023543225.1 (WPP domain-associated protein-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1243 bits (3215), Expect = 0.0
Identity = 688/884 (77.83%), Postives = 766/884 (86.65%), Query Frame = 0

Query: 1   MDANATPEENGNMQLSASDKQLEDLGDDIQILEDLDSYLEDIDDRLTISRMVSDAVIKGM 60
           MDANAT E+NGNMQL+ SDKQ ++LGDD   LED DSY +DI+DRLTISRMVSD+VIKGM
Sbjct: 1   MDANATLEDNGNMQLTGSDKQDDNLGDDF--LEDFDSYWQDINDRLTISRMVSDSVIKGM 60

Query: 61  VNAISEEANEKITRKESEVSELREILKCYHLGSEKNKSLVSPLRSNKSKSTKCDRNEGIS 120
           VNA+S+EA+EKIT+KE EVSEL+EI+  Y L SEK K + SPLR  K KST+CDR + I 
Sbjct: 61  VNAVSQEAHEKITQKELEVSELKEIMHSYLLDSEKTKFMTSPLRLCKLKSTECDRIDSIC 120

Query: 121 GALFEHDEMRDSVCNLKNAAKENFKKLRKEIDKIRGCSSIRKINSGSELVGL--SGILQE 180
           GA FEHDEMR+S+C+L+N AKENFKKL+KEID IRGC+S RKINSGSELVGL  SGILQE
Sbjct: 121 GAFFEHDEMRESMCSLRNTAKENFKKLKKEIDGIRGCNSSRKINSGSELVGLGLSGILQE 180

Query: 181 KASSRCVDVDKIVDDLQDNLDTFYNQVEDIVQVSKASLGQWQDEKEFLADIEGMVIRNYI 240
           KAS R +DV KIVDDLQDNLDTFYNQVEDIVQ+SKAS GQWQ E+E+LADIEGMVIRNYI
Sbjct: 181 KASCRYIDVGKIVDDLQDNLDTFYNQVEDIVQLSKASFGQWQVEQEYLADIEGMVIRNYI 240

Query: 241 WSVQQELEEKLWDQNAKIFSNERKIWVQKIKEISSLRQELDIISKSLSP-EVGHLFSYSS 300
           WS+QQE EEKLWDQNAKI SNERKI  +K+KEISSLRQELD ISKSL P EVGHL S SS
Sbjct: 241 WSIQQEFEEKLWDQNAKILSNERKISAEKMKEISSLRQELDKISKSLCPSEVGHLISCSS 300

Query: 301 MDSDHSHRKLLSNHMAPST----GNGKHELSKTNLPENVDPARLKHLNRDELINHFNTEM 360
           MDSDHSHRKLLS+HM PST    GNGKHE+SKTNLPENVDP+RL H+ +DELINHFNT M
Sbjct: 301 MDSDHSHRKLLSSHMNPSTLHWEGNGKHEMSKTNLPENVDPSRLNHMKKDELINHFNTVM 360

Query: 361 TKMSRNHESLVHEITEENFTLKRELLKEKEKSSL-KKDKEFDILRKKIPDIILKLDDILM 420
           TKMSRNHES VHEITEENF LKR LLKE+EKSSL KKDKEFDILR+KIPDIILKLDD+L+
Sbjct: 361 TKMSRNHESQVHEITEENFILKRVLLKEREKSSLLKKDKEFDILRRKIPDIILKLDDLLL 420

Query: 421 ENEKLSVSAANDENLGTMRNRLESLIAENRHLKDSLEEKKKEVKCLSLQISSSSEKMSQQ 480
           ENEKL  S+ANDE+LGTMRNRLESLI+EN HLKD L EKKKEVKCLS Q+SS +EKMSQ 
Sbjct: 421 ENEKLRSSSANDESLGTMRNRLESLISENHHLKDLLGEKKKEVKCLSSQVSSHAEKMSQY 480

Query: 481 SLRHAKSLNTIEKIKCEMQDAKFEASIGEDIVKCFLRETMDTIRCVNEESALRYDIMQGI 540
           SL  ++SL TIEK KCEMQDA+FEASI +DI KCFLR+ MD +RC  EESA RYDIMQG+
Sbjct: 481 SLLVSESLTTIEKNKCEMQDAQFEASICQDIFKCFLRDMMDQVRCATEESAWRYDIMQGV 540

Query: 541 YETIFQGASFRGELASTSEDEHLDEESIIMQGILGVVLQESWKEAEEKMTSLNNRYIEEM 600
           YETIF+GASF GELASTSE+EHLDEESI MQG+LGVVLQES KEAEEKMTSLNNRY+EEM
Sbjct: 541 YETIFEGASFVGELASTSENEHLDEESITMQGLLGVVLQESLKEAEEKMTSLNNRYMEEM 600

Query: 601 RIRQSLEKEVLHCGEALRVEIFEKQKLQAELVLLGASLKEKEQLVQEITSALEKEKGKLA 660
             R SLEKE+LH GEALRVE+ EK+ L+AEL+   ASLKEKEQLVQEIT  LEKE+ KLA
Sbjct: 601 STRLSLEKEMLHSGEALRVEVSEKEMLEAELISFRASLKEKEQLVQEITFVLEKEREKLA 660

Query: 661 LAYEEVGSLKDQTNRQETLILKGHEESNITQLKLADALRKVEFLEVETCKSKQNLEQAMM 720
           LAYEEVGSLKDQTNRQE +ILK HEE NIT+LKL +A++KV  LEVETC+SKQN EQAM 
Sbjct: 661 LAYEEVGSLKDQTNRQEIMILKSHEELNITKLKLTEAIQKVGLLEVETCESKQNFEQAMA 720

Query: 721 ELSKVDEERRMLVARLLESQKTELLVEEKEKESRKQMESVIILVQELLKEVFDFEHRAID 780
           E SKVDEERRML A + E++KT+LLVEEKEKESRKQMESVI +VQELLKEVFDFEHR ID
Sbjct: 721 EFSKVDEERRMLAAMVSENKKTKLLVEEKEKESRKQMESVIFVVQELLKEVFDFEHRVID 780

Query: 781 YISRNNKRLESLSSQTNSLIQNACMLKRAGLIYKQRHEKRCSDLQKAEAEVDLLGDEVDA 840
           YISRNN RLE LSS+T SLIQ A  +KR GL+YKQ+ EKRCSDLQKAEAEVDLLGDEV A
Sbjct: 781 YISRNNGRLERLSSETKSLIQKASTVKREGLLYKQKLEKRCSDLQKAEAEVDLLGDEVAA 840

Query: 841 LLRLLEKMYIALDHYSPILQHYPGIVDILKLVKRELSGDTTAAF 876
           LLRLL  M+I LD YSPIL+HYPGIV I+KLVKRELSGD T AF
Sbjct: 841 LLRLLGNMHIELDRYSPILKHYPGIVKIMKLVKRELSGDMTEAF 882

BLAST of MC01g1612 vs. NCBI nr
Match: XP_022926720.1 (WPP domain-associated protein [Cucurbita moschata] >XP_022926722.1 WPP domain-associated protein [Cucurbita moschata])

HSP 1 Score: 1241 bits (3212), Expect = 0.0
Identity = 694/884 (78.51%), Postives = 770/884 (87.10%), Query Frame = 0

Query: 1   MDANATPEENGNMQLSASDKQLEDLGDDIQILEDLDSYLEDIDDRLTISRMVSDAVIKGM 60
           MDANA  EENG M+LS SDKQ EDLGDDI  LEDLDSYL+DI+DRLTISRMVSD+VIKGM
Sbjct: 1   MDANAPLEENGKMELSDSDKQDEDLGDDI--LEDLDSYLQDINDRLTISRMVSDSVIKGM 60

Query: 61  VNAISEEANEKITRKESEVSELREILKCYHLGSEKNKSLVSPLRSNKSKSTKCDRNEGIS 120
           VNAIS+EA+EKIT+KE EVSEL+EI+  YHLGSEKN+ LVSP  S K K ++ DRN+ IS
Sbjct: 61  VNAISQEAHEKITQKELEVSELKEIVHSYHLGSEKNEFLVSPPMSWKPKISEHDRNDSIS 120

Query: 121 GALFEHDEMRDSVCNLKNAAKENFKKLRKEIDKIRGCSSIRKINSGSELVG--LSGILQE 180
           GA FEHDEMR+S+C+LKN+ +ENFKKLRKEID+IRG +SIRKINSGSELVG  LSGILQE
Sbjct: 121 GAFFEHDEMRESMCSLKNSTEENFKKLRKEIDRIRGENSIRKINSGSELVGFGLSGILQE 180

Query: 181 KASSRCVDVDKIVDDLQDNLDTFYNQVEDIVQVSKASLGQWQDEKEFLADIEGMVIRNYI 240
           KASSRC+DVDKIVDDLQDNLDT+Y QVEDIVQ+SKASLGQWQ E+E+L+DIEGMVIRNYI
Sbjct: 181 KASSRCIDVDKIVDDLQDNLDTYYKQVEDIVQLSKASLGQWQVEQEYLSDIEGMVIRNYI 240

Query: 241 WSVQQELEEKLWDQNAKIFSNERKIWVQKIKEISSLRQELDIISKSLSP-EVGHLFSYSS 300
             VQ+E +EKLWDQ AKI SNERKI  +KIKEIS LRQELDII KSLSP E+GHL SYSS
Sbjct: 241 CCVQEEFKEKLWDQKAKILSNERKISAKKIKEISCLRQELDIILKSLSPSEIGHLISYSS 300

Query: 301 MDSDHSHRKLLSNHMAPST----GNGKHELSKTNLPENVDPARLKHLNRDELINHFNTEM 360
           MDSDHSHRKL   HM PST    GNGKHE+SKTNLP NVDPARLKHL RDELINHFN EM
Sbjct: 301 MDSDHSHRKL---HMTPSTLLWEGNGKHEMSKTNLPTNVDPARLKHLTRDELINHFNAEM 360

Query: 361 TKMSRNHESLVHEITEENFTLKRELLKEKEKSSL-KKDKEFDILRKKIPDIILKLDDILM 420
           TKMSRNHES VH+ITEENFTLKRELLKEKEKSSL KKDKEFDILR+KIPDIILKLDDI+M
Sbjct: 361 TKMSRNHESQVHQITEENFTLKRELLKEKEKSSLLKKDKEFDILRRKIPDIILKLDDIVM 420

Query: 421 ENEKLSVSAANDENLGTMRNRLESLIAENRHLKDSLEEKKKEVKCLSLQISSSSEKMSQQ 480
           ENEK+  S ANDENLGTMR RLESLI+EN HL D L EK KEVKCLS Q+SS +E+MSQ 
Sbjct: 421 ENEKIQSSDANDENLGTMRKRLESLISENHHLNDLLGEKIKEVKCLSFQVSSHAEQMSQH 480

Query: 481 SLRHAKSLNTIEKIKCEMQDAKFEASIGEDIVKCFLRETMDTIRCVNEESALRYDIMQGI 540
           SL H KSLNTIE+I CEMQDA+FEASI EDIVKCFLRET+D IR   EESALRYDIMQGI
Sbjct: 481 SLLHVKSLNTIERITCEMQDAQFEASICEDIVKCFLRETIDQIRYTPEESALRYDIMQGI 540

Query: 541 YETIFQGASFRGELASTSEDEHLDEESIIMQGILGVVLQESWKEAEEKMTSLNNRYIEEM 600
           Y+TI QGASF GEL S+SE+EHLDEES+IMQ ILGVVLQES KEAEEKM+SLN+RY+EEM
Sbjct: 541 YKTILQGASFIGELPSSSENEHLDEESVIMQSILGVVLQESLKEAEEKMSSLNSRYMEEM 600

Query: 601 RIRQSLEKEVLHCGEALRVEIFEKQKLQAELVLLGASLKEKEQLVQEITSALEKEKGKLA 660
           RIR SLE +V HCGEAL V  FEK+KL+AEL+ L +SLKEKEQ VQEI   LEKEK K+A
Sbjct: 601 RIRLSLETKVFHCGEALGVAAFEKEKLEAELLSLRSSLKEKEQRVQEIIFELEKEKEKVA 660

Query: 661 LAYEEVGSLKDQTNRQETLILKGHEESNITQLKLADALRKVEFLEVETCKSKQNLEQAMM 720
           LAYEEV SLKDQTN QE LILK  EESN   LKL +A++KVE LEVETC+SKQNLEQAM 
Sbjct: 661 LAYEEVDSLKDQTNMQEILILKSQEESNTANLKLTEAMQKVELLEVETCESKQNLEQAMA 720

Query: 721 ELSKVDEERRMLVARLLESQKTELLVEEKEKESRKQMESVIILVQELLKEVFDFEHRAID 780
           ELSKVDEERRML A + E ++T LLVEEKEKES+KQ+ESVI +VQ+LLKEVFDFEHR +D
Sbjct: 721 ELSKVDEERRMLAAMVSEHKETMLLVEEKEKESKKQIESVIFVVQDLLKEVFDFEHRVVD 780

Query: 781 YISRNNKRLESLSSQTNSLIQNACMLKRAGLIYKQRHEKRCSDLQKAEAEVDLLGDEVDA 840
           YISRNN+RLESLSS+T SL+QNA MLKRA L++KQR EKRCSDLQKAEAEVDLLGDEVDA
Sbjct: 781 YISRNNERLESLSSETKSLVQNASMLKRASLLFKQRLEKRCSDLQKAEAEVDLLGDEVDA 840

Query: 841 LLRLLEKMYIALDHYSPILQHYPGIVDILKLVKRELSGDTTAAF 876
           LLRLLEKMY ALD YSPIL+HYPGIV +LKL+KRELS +TT AF
Sbjct: 841 LLRLLEKMYNALDSYSPILKHYPGIVKVLKLLKRELSRNTTEAF 879

BLAST of MC01g1612 vs. ExPASy TrEMBL
Match: A0A6J1CYZ6 (WPP domain-associated protein OS=Momordica charantia OX=3673 GN=LOC111015761 PE=4 SV=1)

HSP 1 Score: 1624 bits (4205), Expect = 0.0
Identity = 873/876 (99.66%), Postives = 874/876 (99.77%), Query Frame = 0

Query: 1   MDANATPEENGNMQLSASDKQLEDLGDDIQILEDLDSYLEDIDDRLTISRMVSDAVIKGM 60
           MDANATPEENGNMQLSASDKQLEDLGDDIQILEDLDSYLEDIDDRLTISRMVSDAVIKGM
Sbjct: 1   MDANATPEENGNMQLSASDKQLEDLGDDIQILEDLDSYLEDIDDRLTISRMVSDAVIKGM 60

Query: 61  VNAISEEANEKITRKESEVSELREILKCYHLGSEKNKSLVSPLRSNKSKSTKCDRNEGIS 120
           VNAISEEANEKITRKESEVSELREILKCYHLGSEKNKSLVSPLRSNKSKSTKCDRNEGIS
Sbjct: 61  VNAISEEANEKITRKESEVSELREILKCYHLGSEKNKSLVSPLRSNKSKSTKCDRNEGIS 120

Query: 121 GALFEHDEMRDSVCNLKNAAKENFKKLRKEIDKIRGCSSIRKINSGSELVGLSGILQEKA 180
           GALFEHDEMRDSVCNLKNAAKENFKKLRKEIDKIRGCSSIRKINSGSELVGLSGILQEKA
Sbjct: 121 GALFEHDEMRDSVCNLKNAAKENFKKLRKEIDKIRGCSSIRKINSGSELVGLSGILQEKA 180

Query: 181 SSRCVDVDKIVDDLQDNLDTFYNQVEDIVQVSKASLGQWQDEKEFLADIEGMVIRNYIWS 240
           SSRCVDVDKIVDDLQDNLDTFYNQVEDIVQVSKASLGQWQDEKEFLADIEGMVIRNYIWS
Sbjct: 181 SSRCVDVDKIVDDLQDNLDTFYNQVEDIVQVSKASLGQWQDEKEFLADIEGMVIRNYIWS 240

Query: 241 VQQELEEKLWDQNAKIFSNERKIWVQKIKEISSLRQELDIISKSLSPEVGHLFSYSSMDS 300
           VQQELEEKLWDQNAKIFSNERKIWVQKIKEISSLRQELDIISKSLSPEVGHLFSYSSMDS
Sbjct: 241 VQQELEEKLWDQNAKIFSNERKIWVQKIKEISSLRQELDIISKSLSPEVGHLFSYSSMDS 300

Query: 301 DHSHRKLLSNHMAPSTGNGKHELSKTNLPENVDPARLKHLNRDELINHFNTEMTKMSRNH 360
           DHSHRKLLSNHMAPSTGNGKHE+SKTNLPENVDPARLKHLNRDELINHFNTEMTKMSRNH
Sbjct: 301 DHSHRKLLSNHMAPSTGNGKHEMSKTNLPENVDPARLKHLNRDELINHFNTEMTKMSRNH 360

Query: 361 ESLVHEITEENFTLKRELLKEKEKSSLKKDKEFDILRKKIPDIILKLDDILMENEKLSVS 420
           ESLVHEITEENFTLKRELLKEKEKSSLKKDKEFDILRKKIPDIILKLDDILMENEKLSVS
Sbjct: 361 ESLVHEITEENFTLKRELLKEKEKSSLKKDKEFDILRKKIPDIILKLDDILMENEKLSVS 420

Query: 421 AANDENLGTMRNRLESLIAENRHLKDSLEEKKKEVKCLSLQISSSSEKMSQQSLRHAKSL 480
           AANDENLGTMRNRLESLIAENRHLKDSLEEKKKEVKCLSLQISSSSEKMSQQSLRH KSL
Sbjct: 421 AANDENLGTMRNRLESLIAENRHLKDSLEEKKKEVKCLSLQISSSSEKMSQQSLRHGKSL 480

Query: 481 NTIEKIKCEMQDAKFEASIGEDIVKCFLRETMDTIRCVNEESALRYDIMQGIYETIFQGA 540
           NTIEKIKCEMQDAKFEASIGEDIVKCFLRETMDTIRCVNEESALRYDIMQGIYETIFQGA
Sbjct: 481 NTIEKIKCEMQDAKFEASIGEDIVKCFLRETMDTIRCVNEESALRYDIMQGIYETIFQGA 540

Query: 541 SFRGELASTSEDEHLDEESIIMQGILGVVLQESWKEAEEKMTSLNNRYIEEMRIRQSLEK 600
           SFRGELASTSEDEHLDEESIIMQGILGVVLQESWKEAEEKMTSLNNRYIEEMRIRQSLEK
Sbjct: 541 SFRGELASTSEDEHLDEESIIMQGILGVVLQESWKEAEEKMTSLNNRYIEEMRIRQSLEK 600

Query: 601 EVLHCGEALRVEIFEKQKLQAELVLLGASLKEKEQLVQEITSALEKEKGKLALAYEEVGS 660
           EVLHCGEALRVEIFEKQKLQAELVLLGASLKEKEQLVQEITSALEKEKGKLALAYEEVGS
Sbjct: 601 EVLHCGEALRVEIFEKQKLQAELVLLGASLKEKEQLVQEITSALEKEKGKLALAYEEVGS 660

Query: 661 LKDQTNRQETLILKGHEESNITQLKLADALRKVEFLEVETCKSKQNLEQAMMELSKVDEE 720
           LKDQTNRQETLILKGHEESNITQLKLADALRKVEFLEVETCKSKQNLEQAMMELSKVDEE
Sbjct: 661 LKDQTNRQETLILKGHEESNITQLKLADALRKVEFLEVETCKSKQNLEQAMMELSKVDEE 720

Query: 721 RRMLVARLLESQKTELLVEEKEKESRKQMESVIILVQELLKEVFDFEHRAIDYISRNNKR 780
           RRMLVARLLESQKTELLVEEKEKESRKQMESVIILVQELLKEVFDFEH AIDYISRNNKR
Sbjct: 721 RRMLVARLLESQKTELLVEEKEKESRKQMESVIILVQELLKEVFDFEHTAIDYISRNNKR 780

Query: 781 LESLSSQTNSLIQNACMLKRAGLIYKQRHEKRCSDLQKAEAEVDLLGDEVDALLRLLEKM 840
           LESLSSQTNSLIQNACMLKRAGLIYKQRHEKRCSDLQKAEAEVDLLGDEVDALLRLLEKM
Sbjct: 781 LESLSSQTNSLIQNACMLKRAGLIYKQRHEKRCSDLQKAEAEVDLLGDEVDALLRLLEKM 840

Query: 841 YIALDHYSPILQHYPGIVDILKLVKRELSGDTTAAF 876
           YIALDHYSPILQHYPGIVDILKLVKRELSGDTTAAF
Sbjct: 841 YIALDHYSPILQHYPGIVDILKLVKRELSGDTTAAF 876

BLAST of MC01g1612 vs. ExPASy TrEMBL
Match: A0A6J1ED14 (WPP domain-associated protein isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111433065 PE=4 SV=1)

HSP 1 Score: 1249 bits (3231), Expect = 0.0
Identity = 694/884 (78.51%), Postives = 770/884 (87.10%), Query Frame = 0

Query: 1   MDANATPEENGNMQLSASDKQLEDLGDDIQILEDLDSYLEDIDDRLTISRMVSDAVIKGM 60
           MDANAT E+NGN+QL+ SDKQ ++LGDD   LED DSY +DI+DRLTISRMVSD+VIK M
Sbjct: 1   MDANATLEDNGNIQLTGSDKQDDNLGDDF--LEDFDSYWQDINDRLTISRMVSDSVIKVM 60

Query: 61  VNAISEEANEKITRKESEVSELREILKCYHLGSEKNKSLVSPLRSNKSKSTKCDRNEGIS 120
           VNA+S+EA+EKIT+ E EVSEL+EI+  Y LGSEK K + SPLR  K KST+CDR + I 
Sbjct: 61  VNAVSQEAHEKITQNELEVSELKEIIHSYLLGSEKTKFMTSPLRLCKLKSTECDRIDSIC 120

Query: 121 GALFEHDEMRDSVCNLKNAAKENFKKLRKEIDKIRGCSSIRKINSGSELVGL--SGILQE 180
           GA FEHDEMR+S+C+L+N AKENFKKL+KEID IRGC+S RKINSGSELVGL  SGILQE
Sbjct: 121 GAFFEHDEMRESMCSLRNTAKENFKKLKKEIDGIRGCNSSRKINSGSELVGLGLSGILQE 180

Query: 181 KASSRCVDVDKIVDDLQDNLDTFYNQVEDIVQVSKASLGQWQDEKEFLADIEGMVIRNYI 240
           KASSR +DV KIVDDLQDNLDTFYNQVED+VQ+SKAS GQWQ E+E+LADIEGMVIRNYI
Sbjct: 181 KASSRYIDVGKIVDDLQDNLDTFYNQVEDLVQLSKASFGQWQVEQEYLADIEGMVIRNYI 240

Query: 241 WSVQQELEEKLWDQNAKIFSNERKIWVQKIKEISSLRQELDIISKSLSP-EVGHLFSYSS 300
           WS+QQE EEKLWDQNAKI SNERKI  +KIKEISSLRQELD ISKSL P EVGHL S SS
Sbjct: 241 WSIQQEFEEKLWDQNAKILSNERKISAEKIKEISSLRQELDKISKSLCPSEVGHLISCSS 300

Query: 301 MDSDHSHRKLLSNHMAPST----GNGKHELSKTNLPENVDPARLKHLNRDELINHFNTEM 360
           MDSDHSHRKLLS+HM PST    GN KHE+SKTNLPENVDP+RL H+ +DELINHFNT M
Sbjct: 301 MDSDHSHRKLLSSHMNPSTLHWEGNCKHEMSKTNLPENVDPSRLNHMKKDELINHFNTVM 360

Query: 361 TKMSRNHESLVHEITEENFTLKRELLKEKEKSSL-KKDKEFDILRKKIPDIILKLDDILM 420
           TKMSRNHES VHEITEENFTLKRELLK++EKSSL KKDKEFDILR+KIPDIILKLDDIL+
Sbjct: 361 TKMSRNHESQVHEITEENFTLKRELLKKREKSSLLKKDKEFDILRRKIPDIILKLDDILL 420

Query: 421 ENEKLSVSAANDENLGTMRNRLESLIAENRHLKDSLEEKKKEVKCLSLQISSSSEKMSQQ 480
           ENEKL  S+ANDE+LGTMRNRLESLI+EN HLKD L EKKKEVKCLS Q+SS +EKMSQ 
Sbjct: 421 ENEKLRSSSANDESLGTMRNRLESLISENHHLKDLLGEKKKEVKCLSSQVSSHAEKMSQH 480

Query: 481 SLRHAKSLNTIEKIKCEMQDAKFEASIGEDIVKCFLRETMDTIRCVNEESALRYDIMQGI 540
           SL  ++SL TIEK KCEMQDA+FEASI +DI KCFLRE MD IR   EESA RYDIMQG+
Sbjct: 481 SLLVSESLTTIEKNKCEMQDAQFEASICQDIFKCFLREMMDQIRYATEESAWRYDIMQGV 540

Query: 541 YETIFQGASFRGELASTSEDEHLDEESIIMQGILGVVLQESWKEAEEKMTSLNNRYIEEM 600
           YETI++GASF GELAST+E+EHLDEESI MQG+LGVVLQES KEAEEKMTSLNNRY+EEM
Sbjct: 541 YETIYEGASFVGELASTTENEHLDEESITMQGLLGVVLQESLKEAEEKMTSLNNRYMEEM 600

Query: 601 RIRQSLEKEVLHCGEALRVEIFEKQKLQAELVLLGASLKEKEQLVQEITSALEKEKGKLA 660
             R SLEKE+LH GEALRVE+FEK+ L+AEL+   ASL+EKEQLVQEIT  LEKEK KLA
Sbjct: 601 STRLSLEKEMLHSGEALRVEVFEKEMLEAELISFRASLREKEQLVQEITFVLEKEKEKLA 660

Query: 661 LAYEEVGSLKDQTNRQETLILKGHEESNITQLKLADALRKVEFLEVETCKSKQNLEQAMM 720
           LAYEEVGSLKDQTNRQE LILK HEE NIT+LKL +A+RKV  LEVETC+SKQNLEQAM 
Sbjct: 661 LAYEEVGSLKDQTNRQEILILKSHEELNITKLKLTEAMRKVGLLEVETCESKQNLEQAMA 720

Query: 721 ELSKVDEERRMLVARLLESQKTELLVEEKEKESRKQMESVIILVQELLKEVFDFEHRAID 780
           E SKVDEERRMLVA + E+QKT+LLVEEKEKESRKQMESVI +VQELLKEVFDFEHR ID
Sbjct: 721 EFSKVDEERRMLVAMVSENQKTKLLVEEKEKESRKQMESVIFVVQELLKEVFDFEHRVID 780

Query: 781 YISRNNKRLESLSSQTNSLIQNACMLKRAGLIYKQRHEKRCSDLQKAEAEVDLLGDEVDA 840
           YISRN  RLE LSS+T SLIQ A M+KR GL+YKQ+ EKRCSDLQKAEAEVDLLGDEV A
Sbjct: 781 YISRNKGRLERLSSETKSLIQKASMVKREGLLYKQKLEKRCSDLQKAEAEVDLLGDEVAA 840

Query: 841 LLRLLEKMYIALDHYSPILQHYPGIVDILKLVKRELSGDTTAAF 876
           LLRLL KM+I LD YSPIL+HYPGIV I+KLVKRELSGD T AF
Sbjct: 841 LLRLLGKMHIELDRYSPILKHYPGIVKIMKLVKRELSGDMTEAF 882

BLAST of MC01g1612 vs. ExPASy TrEMBL
Match: A0A6J1EF64 (WPP domain-associated protein OS=Cucurbita moschata OX=3662 GN=LOC111433760 PE=4 SV=1)

HSP 1 Score: 1241 bits (3212), Expect = 0.0
Identity = 694/884 (78.51%), Postives = 770/884 (87.10%), Query Frame = 0

Query: 1   MDANATPEENGNMQLSASDKQLEDLGDDIQILEDLDSYLEDIDDRLTISRMVSDAVIKGM 60
           MDANA  EENG M+LS SDKQ EDLGDDI  LEDLDSYL+DI+DRLTISRMVSD+VIKGM
Sbjct: 1   MDANAPLEENGKMELSDSDKQDEDLGDDI--LEDLDSYLQDINDRLTISRMVSDSVIKGM 60

Query: 61  VNAISEEANEKITRKESEVSELREILKCYHLGSEKNKSLVSPLRSNKSKSTKCDRNEGIS 120
           VNAIS+EA+EKIT+KE EVSEL+EI+  YHLGSEKN+ LVSP  S K K ++ DRN+ IS
Sbjct: 61  VNAISQEAHEKITQKELEVSELKEIVHSYHLGSEKNEFLVSPPMSWKPKISEHDRNDSIS 120

Query: 121 GALFEHDEMRDSVCNLKNAAKENFKKLRKEIDKIRGCSSIRKINSGSELVG--LSGILQE 180
           GA FEHDEMR+S+C+LKN+ +ENFKKLRKEID+IRG +SIRKINSGSELVG  LSGILQE
Sbjct: 121 GAFFEHDEMRESMCSLKNSTEENFKKLRKEIDRIRGENSIRKINSGSELVGFGLSGILQE 180

Query: 181 KASSRCVDVDKIVDDLQDNLDTFYNQVEDIVQVSKASLGQWQDEKEFLADIEGMVIRNYI 240
           KASSRC+DVDKIVDDLQDNLDT+Y QVEDIVQ+SKASLGQWQ E+E+L+DIEGMVIRNYI
Sbjct: 181 KASSRCIDVDKIVDDLQDNLDTYYKQVEDIVQLSKASLGQWQVEQEYLSDIEGMVIRNYI 240

Query: 241 WSVQQELEEKLWDQNAKIFSNERKIWVQKIKEISSLRQELDIISKSLSP-EVGHLFSYSS 300
             VQ+E +EKLWDQ AKI SNERKI  +KIKEIS LRQELDII KSLSP E+GHL SYSS
Sbjct: 241 CCVQEEFKEKLWDQKAKILSNERKISAKKIKEISCLRQELDIILKSLSPSEIGHLISYSS 300

Query: 301 MDSDHSHRKLLSNHMAPST----GNGKHELSKTNLPENVDPARLKHLNRDELINHFNTEM 360
           MDSDHSHRKL   HM PST    GNGKHE+SKTNLP NVDPARLKHL RDELINHFN EM
Sbjct: 301 MDSDHSHRKL---HMTPSTLLWEGNGKHEMSKTNLPTNVDPARLKHLTRDELINHFNAEM 360

Query: 361 TKMSRNHESLVHEITEENFTLKRELLKEKEKSSL-KKDKEFDILRKKIPDIILKLDDILM 420
           TKMSRNHES VH+ITEENFTLKRELLKEKEKSSL KKDKEFDILR+KIPDIILKLDDI+M
Sbjct: 361 TKMSRNHESQVHQITEENFTLKRELLKEKEKSSLLKKDKEFDILRRKIPDIILKLDDIVM 420

Query: 421 ENEKLSVSAANDENLGTMRNRLESLIAENRHLKDSLEEKKKEVKCLSLQISSSSEKMSQQ 480
           ENEK+  S ANDENLGTMR RLESLI+EN HL D L EK KEVKCLS Q+SS +E+MSQ 
Sbjct: 421 ENEKIQSSDANDENLGTMRKRLESLISENHHLNDLLGEKIKEVKCLSFQVSSHAEQMSQH 480

Query: 481 SLRHAKSLNTIEKIKCEMQDAKFEASIGEDIVKCFLRETMDTIRCVNEESALRYDIMQGI 540
           SL H KSLNTIE+I CEMQDA+FEASI EDIVKCFLRET+D IR   EESALRYDIMQGI
Sbjct: 481 SLLHVKSLNTIERITCEMQDAQFEASICEDIVKCFLRETIDQIRYTPEESALRYDIMQGI 540

Query: 541 YETIFQGASFRGELASTSEDEHLDEESIIMQGILGVVLQESWKEAEEKMTSLNNRYIEEM 600
           Y+TI QGASF GEL S+SE+EHLDEES+IMQ ILGVVLQES KEAEEKM+SLN+RY+EEM
Sbjct: 541 YKTILQGASFIGELPSSSENEHLDEESVIMQSILGVVLQESLKEAEEKMSSLNSRYMEEM 600

Query: 601 RIRQSLEKEVLHCGEALRVEIFEKQKLQAELVLLGASLKEKEQLVQEITSALEKEKGKLA 660
           RIR SLE +V HCGEAL V  FEK+KL+AEL+ L +SLKEKEQ VQEI   LEKEK K+A
Sbjct: 601 RIRLSLETKVFHCGEALGVAAFEKEKLEAELLSLRSSLKEKEQRVQEIIFELEKEKEKVA 660

Query: 661 LAYEEVGSLKDQTNRQETLILKGHEESNITQLKLADALRKVEFLEVETCKSKQNLEQAMM 720
           LAYEEV SLKDQTN QE LILK  EESN   LKL +A++KVE LEVETC+SKQNLEQAM 
Sbjct: 661 LAYEEVDSLKDQTNMQEILILKSQEESNTANLKLTEAMQKVELLEVETCESKQNLEQAMA 720

Query: 721 ELSKVDEERRMLVARLLESQKTELLVEEKEKESRKQMESVIILVQELLKEVFDFEHRAID 780
           ELSKVDEERRML A + E ++T LLVEEKEKES+KQ+ESVI +VQ+LLKEVFDFEHR +D
Sbjct: 721 ELSKVDEERRMLAAMVSEHKETMLLVEEKEKESKKQIESVIFVVQDLLKEVFDFEHRVVD 780

Query: 781 YISRNNKRLESLSSQTNSLIQNACMLKRAGLIYKQRHEKRCSDLQKAEAEVDLLGDEVDA 840
           YISRNN+RLESLSS+T SL+QNA MLKRA L++KQR EKRCSDLQKAEAEVDLLGDEVDA
Sbjct: 781 YISRNNERLESLSSETKSLVQNASMLKRASLLFKQRLEKRCSDLQKAEAEVDLLGDEVDA 840

Query: 841 LLRLLEKMYIALDHYSPILQHYPGIVDILKLVKRELSGDTTAAF 876
           LLRLLEKMY ALD YSPIL+HYPGIV +LKL+KRELS +TT AF
Sbjct: 841 LLRLLEKMYNALDSYSPILKHYPGIVKVLKLLKRELSRNTTEAF 879

BLAST of MC01g1612 vs. ExPASy TrEMBL
Match: A0A6J1IQ29 (WPP domain-associated protein-like OS=Cucurbita maxima OX=3661 GN=LOC111477803 PE=4 SV=1)

HSP 1 Score: 1235 bits (3195), Expect = 0.0
Identity = 686/884 (77.60%), Postives = 768/884 (86.88%), Query Frame = 0

Query: 1   MDANATPEENGNMQLSASDKQLEDLGDDIQILEDLDSYLEDIDDRLTISRMVSDAVIKGM 60
           MDANAT E+NGN+QL+ +DKQ +  GDD   LED DSY +DI+DRLTISRMVSD+VIKGM
Sbjct: 1   MDANATLEDNGNIQLTGTDKQDDYPGDDF--LEDFDSYWQDINDRLTISRMVSDSVIKGM 60

Query: 61  VNAISEEANEKITRKESEVSELREILKCYHLGSEKNKSLVSPLRSNKSKSTKCDRNEGIS 120
           VNA+S+EA+EKIT+KE EVSEL+EI+  Y L SEK K ++SPLR  K KST+CDR + I 
Sbjct: 61  VNAVSQEAHEKITQKELEVSELKEIIHSYLLDSEKTKFMMSPLRLCKLKSTECDRIDSIC 120

Query: 121 GALFEHDEMRDSVCNLKNAAKENFKKLRKEIDKIRGCSSIRKINSGSELVGL--SGILQE 180
           GA FEHDEMR+S+C+L+N AKENFKKL+KE+D IRGC+S RKINSGSELVGL  SGILQE
Sbjct: 121 GAFFEHDEMRESMCSLRNTAKENFKKLKKELDGIRGCNSSRKINSGSELVGLGLSGILQE 180

Query: 181 KASSRCVDVDKIVDDLQDNLDTFYNQVEDIVQVSKASLGQWQDEKEFLADIEGMVIRNYI 240
           KASSR +DV +IVDDLQDNLDTFYNQVEDIVQ+SKAS GQWQ E+E+LADIEGMVIRNYI
Sbjct: 181 KASSRYIDVGRIVDDLQDNLDTFYNQVEDIVQLSKASFGQWQVEQEYLADIEGMVIRNYI 240

Query: 241 WSVQQELEEKLWDQNAKIFSNERKIWVQKIKEISSLRQELDIISKSLSP-EVGHLFSYSS 300
           WS+QQ+ EEKLWDQNAKI SNERKI  +K+KEISSL QEL  ISKSL P EVGHL S SS
Sbjct: 241 WSIQQDFEEKLWDQNAKILSNERKISAEKMKEISSLCQELGKISKSLCPSEVGHLISCSS 300

Query: 301 MDSDHSHRKLLSNHMAPST----GNGKHELSKTNLPENVDPARLKHLNRDELINHFNTEM 360
            DSDHS RKLLS+HM PST    GNGKHE+SKTNLPENVDP+RL H+ +DELINHFNT M
Sbjct: 301 TDSDHSQRKLLSSHMNPSTLHWEGNGKHEMSKTNLPENVDPSRLNHMKKDELINHFNTVM 360

Query: 361 TKMSRNHESLVHEITEENFTLKRELLKEKEKSSL-KKDKEFDILRKKIPDIILKLDDILM 420
           TKMSRNHES VHEITEENFTLKRE+LKE+EKSSL KKDKEFDILR+KIPDIILKLDDIL+
Sbjct: 361 TKMSRNHESQVHEITEENFTLKREILKEREKSSLLKKDKEFDILRRKIPDIILKLDDILL 420

Query: 421 ENEKLSVSAANDENLGTMRNRLESLIAENRHLKDSLEEKKKEVKCLSLQISSSSEKMSQQ 480
           ENEKL +S+AN+E+LGTMRNRLESLI+EN HLKD L EKKKEVKCLS Q+SS +EKMSQ 
Sbjct: 421 ENEKLRLSSANNESLGTMRNRLESLISENHHLKDLLGEKKKEVKCLSSQVSSHAEKMSQH 480

Query: 481 SLRHAKSLNTIEKIKCEMQDAKFEASIGEDIVKCFLRETMDTIRCVNEESALRYDIMQGI 540
           SL  ++SL TIEK KCEMQDA+FEASI +DI KCFLRE MD IRC  EESA RYDIMQG+
Sbjct: 481 SLLVSESLTTIEKNKCEMQDAQFEASICQDIFKCFLREMMDQIRCATEESAWRYDIMQGV 540

Query: 541 YETIFQGASFRGELASTSEDEHLDEESIIMQGILGVVLQESWKEAEEKMTSLNNRYIEEM 600
           YETIF+GASF GELASTSEDEHLDEESI MQ +LGVVLQES KEAEEKMTSLNNRY+EEM
Sbjct: 541 YETIFEGASFLGELASTSEDEHLDEESITMQCLLGVVLQESLKEAEEKMTSLNNRYMEEM 600

Query: 601 RIRQSLEKEVLHCGEALRVEIFEKQKLQAELVLLGASLKEKEQLVQEITSALEKEKGKLA 660
             R SLEKE+LH GEALRVE+ EK+ L+AEL+ L A LKEKEQLVQEIT  LEKEK KLA
Sbjct: 601 STRLSLEKEMLHSGEALRVEVSEKEMLEAELISLRALLKEKEQLVQEITFVLEKEKAKLA 660

Query: 661 LAYEEVGSLKDQTNRQETLILKGHEESNITQLKLADALRKVEFLEVETCKSKQNLEQAMM 720
           LAY+EVGSLKDQT+RQE LILK HEE NIT+LKL +A++KV  LEVETC+SKQNLEQAM 
Sbjct: 661 LAYKEVGSLKDQTSRQEILILKSHEELNITKLKLTEAMQKVGLLEVETCESKQNLEQAMA 720

Query: 721 ELSKVDEERRMLVARLLESQKTELLVEEKEKESRKQMESVIILVQELLKEVFDFEHRAID 780
           E SKVDEERRMLV+ + E+QKT+LLVEEKEKESRKQMESVI +VQELLKEVFDFEHR ID
Sbjct: 721 EFSKVDEERRMLVSMVSENQKTKLLVEEKEKESRKQMESVIFVVQELLKEVFDFEHRVID 780

Query: 781 YISRNNKRLESLSSQTNSLIQNACMLKRAGLIYKQRHEKRCSDLQKAEAEVDLLGDEVDA 840
           YISRNN RLE LSS+T SLIQ A M+KR GL+YKQ+ EKRCSDLQKAEAEVDLLGDEV A
Sbjct: 781 YISRNNGRLERLSSETKSLIQKASMVKREGLLYKQKLEKRCSDLQKAEAEVDLLGDEVAA 840

Query: 841 LLRLLEKMYIALDHYSPILQHYPGIVDILKLVKRELSGDTTAAF 876
           LLRLL KM+I LD YSPIL+HYPGIV I+KLVKRELSGD T AF
Sbjct: 841 LLRLLGKMHIELDRYSPILKHYPGIVKIMKLVKRELSGDMTEAF 882

BLAST of MC01g1612 vs. ExPASy TrEMBL
Match: A0A6J1KM60 (WPP domain-associated protein-like OS=Cucurbita maxima OX=3661 GN=LOC111496974 PE=4 SV=1)

HSP 1 Score: 1229 bits (3180), Expect = 0.0
Identity = 689/884 (77.94%), Postives = 764/884 (86.43%), Query Frame = 0

Query: 1   MDANATPEENGNMQLSASDKQLEDLGDDIQILEDLDSYLEDIDDRLTISRMVSDAVIKGM 60
           MDANA  EENG M+LS SDK  EDLGDDI  LEDLDSYL+DI+DRLTISRMVSD+VIKGM
Sbjct: 1   MDANAPLEENGKMELSDSDKLDEDLGDDI--LEDLDSYLQDINDRLTISRMVSDSVIKGM 60

Query: 61  VNAISEEANEKITRKESEVSELREILKCYHLGSEKNKSLVSPLRSNKSKSTKCDRNEGIS 120
           VNAIS+EA+EKIT+KE EVSEL+EI+  YHLGSEKN+ LVSP  S K KST+ +RN+ IS
Sbjct: 61  VNAISQEAHEKITQKELEVSELKEIVHSYHLGSEKNEFLVSPSTSWKPKSTEHNRNDSIS 120

Query: 121 GALFEHDEMRDSVCNLKNAAKENFKKLRKEIDKIRGCSSIRKINSGSELVGL--SGILQE 180
            A FE+DEMR+S+C+LKN+ +ENFKKLRKEID+IRG +SIRKINSGSELVGL  SGILQE
Sbjct: 121 DAFFEYDEMRESMCSLKNSTEENFKKLRKEIDRIRGENSIRKINSGSELVGLGLSGILQE 180

Query: 181 KASSRCVDVDKIVDDLQDNLDTFYNQVEDIVQVSKASLGQWQDEKEFLADIEGMVIRNYI 240
           KASSRCVDVDKIVDDLQDNLDT+Y QVEDIVQ+SKASLGQWQ E+E+L DIEGMV RNYI
Sbjct: 181 KASSRCVDVDKIVDDLQDNLDTYYKQVEDIVQLSKASLGQWQVEQEYLEDIEGMVFRNYI 240

Query: 241 WSVQQELEEKLWDQNAKIFSNERKIWVQKIKEISSLRQELDIISKSLSP-EVGHLFSYSS 300
             VQ+E EEKLW QNAKI SNERKI  +KIKEIS LRQELDII KSLSP E+GHL SYSS
Sbjct: 241 CCVQEEFEEKLWVQNAKILSNERKISAEKIKEISCLRQELDIILKSLSPSEIGHLISYSS 300

Query: 301 MDSDHSHRKLLSNHMAPST----GNGKHELSKTNLPENVDPARLKHLNRDELINHFNTEM 360
           MDSDHSHRKL   HM PST    GNGKHE+SKTN P NVDPARLKHL RDELINHFN EM
Sbjct: 301 MDSDHSHRKL---HMTPSTLLWEGNGKHEMSKTNHPTNVDPARLKHLTRDELINHFNAEM 360

Query: 361 TKMSRNHESLVHEITEENFTLKRELLKEKEKSSL-KKDKEFDILRKKIPDIILKLDDILM 420
           TKMSRNHES VH+ITEENFTLKRELLKEKEKSSL KKDKEFDILR+KIPDIILKLDDI+M
Sbjct: 361 TKMSRNHESQVHQITEENFTLKRELLKEKEKSSLLKKDKEFDILRRKIPDIILKLDDIVM 420

Query: 421 ENEKLSVSAANDENLGTMRNRLESLIAENRHLKDSLEEKKKEVKCLSLQISSSSEKMSQQ 480
           ENEK+  S ANDENLGTMR RLESL++EN HL D L EK KEVKCLS Q+SS +E++SQ 
Sbjct: 421 ENEKIQSSYANDENLGTMRKRLESLLSENHHLNDLLGEKIKEVKCLSSQVSSHAEQISQH 480

Query: 481 SLRHAKSLNTIEKIKCEMQDAKFEASIGEDIVKCFLRETMDTIRCVNEESALRYDIMQGI 540
           SL H KSLNTIEKI CEMQDA+FEASI EDIVKCFLRE +D IR   EESALRYDI+QGI
Sbjct: 481 SLLHVKSLNTIEKITCEMQDAQFEASICEDIVKCFLREMIDQIRYTTEESALRYDIVQGI 540

Query: 541 YETIFQGASFRGELASTSEDEHLDEESIIMQGILGVVLQESWKEAEEKMTSLNNRYIEEM 600
           Y+TI QGASF GEL S+SE+EHLDEES+IMQGILGVVLQES KEAEEKM+SLN+RY+EEM
Sbjct: 541 YKTILQGASFIGELPSSSENEHLDEESVIMQGILGVVLQESLKEAEEKMSSLNSRYMEEM 600

Query: 601 RIRQSLEKEVLHCGEALRVEIFEKQKLQAELVLLGASLKEKEQLVQEITSALEKEKGKLA 660
           RIR SLE +V HCGEAL V  FEK+KL+AEL+ + +SLKEKEQ VQEIT  LEKEK K+A
Sbjct: 601 RIRLSLEMKVFHCGEALGVAAFEKEKLEAELLSMRSSLKEKEQRVQEITFELEKEKEKVA 660

Query: 661 LAYEEVGSLKDQTNRQETLILKGHEESNITQLKLADALRKVEFLEVETCKSKQNLEQAMM 720
           LAYEEV SLKDQTN QE LILK  EESN   LK  +A++KVE LEVETC+SKQNLEQAM 
Sbjct: 661 LAYEEVDSLKDQTNMQEILILKSQEESNTANLKFTEAMQKVELLEVETCESKQNLEQAMA 720

Query: 721 ELSKVDEERRMLVARLLESQKTELLVEEKEKESRKQMESVIILVQELLKEVFDFEHRAID 780
           ELSKVDEERRML A + E ++T LLVEEKEKESRKQ+ESVI +VQ+LLKEVFDFEHR +D
Sbjct: 721 ELSKVDEERRMLAAMVSEHKETMLLVEEKEKESRKQIESVIFVVQDLLKEVFDFEHRVVD 780

Query: 781 YISRNNKRLESLSSQTNSLIQNACMLKRAGLIYKQRHEKRCSDLQKAEAEVDLLGDEVDA 840
           YISRNN+RLESLSS+T SL+ NA MLKRA  ++KQR EKRCSDLQKAEAEVDLLGDEVDA
Sbjct: 781 YISRNNERLESLSSETKSLVHNASMLKRASFLFKQRLEKRCSDLQKAEAEVDLLGDEVDA 840

Query: 841 LLRLLEKMYIALDHYSPILQHYPGIVDILKLVKRELSGDTTAAF 876
           LLRLLEKMY ALD YSPIL+HYPGIV +LKL+KRELS DTT AF
Sbjct: 841 LLRLLEKMYNALDSYSPILKHYPGIVKVLKLLKRELSRDTTEAF 879

BLAST of MC01g1612 vs. TAIR 10
Match: AT2G34730.1 (myosin heavy chain-related )

HSP 1 Score: 433.7 bits (1114), Expect = 3.5e-121
Identity = 324/893 (36.28%), Postives = 489/893 (54.76%), Query Frame = 0

Query: 1   MDANATPEENGNMQ---------LSASDKQLEDLGDDIQILEDLDSYLEDIDDRLTISRM 60
           M+      ENG+++         L  +    E+   D+  LEDLDSY EDI+DRLTISR+
Sbjct: 1   MEEEVVKSENGSLEFHDDTLSSSLQVNGVLKENENPDVDFLEDLDSYWEDINDRLTISRV 60

Query: 61  VSDAVIKGMVNAISEEANEKITRKESEVSELREILKCYHLGSEKNKSLVSPLRSNKSKST 120
           VSD++I+GMV AI  +A EKI +K+ E+S++RE L  YH+GSE+N+S  S L        
Sbjct: 61  VSDSIIRGMVTAIESDAAEKIAQKDLELSKIRETLLLYHVGSEENESSESRL-------- 120

Query: 121 KCDRNEGISGALFEHDEM-RDSVCNLKNAAKENFKKLRKEIDKIRGCSSIRKINSGSELV 180
                         HDE+ + S  +LK  A++    L +E+  +R    I    SG+ + 
Sbjct: 121 -------------IHDELTQGSSSSLKKKARKQLLMLVEELTNLREYIHIN--GSGATVD 180

Query: 181 GLSGILQEKASSRCVDVDKIVDDLQDNLDTFYNQVEDIVQVSKASLGQWQDEKEFLADIE 240
              G+      +R   VDK++D L+  L+T   +  D+   S      WQ E +F  +IE
Sbjct: 181 DSLGLDSSPHETRSKTVDKMLDSLKSILETVLKRKNDMELPS-----SWQQEHDFQKEIE 240

Query: 241 GMVIRNYIWSVQQELEEKLWDQNAKIFSNERKIWVQKIKEISSLRQELDIISKSL----- 300
             V+ + + S++ E E++L DQ A+ F   R + +  IKEI+ LRQEL+ I KS      
Sbjct: 241 SAVVTSVLRSLKDEYEQRLLDQKAE-FGGNRSLILGNIKEITGLRQELEAIRKSFLDHEN 300

Query: 301 SPEVGHLFSYSSMDSDHSHRKLLSNHMAPSTGNGKHELSKTNL-PENVDPARLKHLNRDE 360
             E G +     ++  H       N ++    NGKHE S T L PE+ +   L+H++ DE
Sbjct: 301 GDEAGEVGDRKRVEQLHRKMSGSLNSVSSVWENGKHEESSTGLIPEHNE--TLRHMSPDE 360

Query: 361 LINHFNTEMTKMSRNHESLVHEITEENFTLKRELLKEKEKSSLK---KDKEFDILRKKIP 420
           +INHF  EM KM R+H+  + E+TE+ FT KR+ L   E+ S     KDKE   L+KKIP
Sbjct: 361 MINHFKIEMNKMKRDHDYKIQELTEQCFTFKRKYLNLTERGSFSFVGKDKELGALKKKIP 420

Query: 421 DIILKLDDILMENEKLSVSAANDENLGTMRNRLESLIAENRHLKDSLEEKKKEVKCLSLQ 480
            +I KLD ILME+EK      ND  L   + +L+SL+ ENR LKDSL             
Sbjct: 421 FVISKLDKILMEDEKFVSEGKNDAGL---KRQLDSLLLENRQLKDSL------------- 480

Query: 481 ISSSSEKMSQQSLRHAKSLNTIEKIKCEMQDAKFEASIGEDIVKCFLRETMDTIRCVNEE 540
            S ++EKMSQ S   A     I K++ +++D++ EASI ED+  CF+ E +  I+C  +E
Sbjct: 481 -SDAAEKMSQLSQAEADHQELIRKLETDVEDSRNEASIYEDVYGCFVTEFVGQIKCTKQE 540

Query: 541 SALRYDIMQGIYETIFQGASFRGELASTSEDEHLDEESIIMQGILGVVLQESWKEAEEKM 600
           + L + +++  YE + +  + +    S  + E    +S++M+    V+ +E+ KEA +K+
Sbjct: 541 TDLEHSMLREAYELLLEDLARKEARKSKEDFEDSCVKSVMMEECCSVIYKEAVKEAHKKI 600

Query: 601 TSLNNRYIEEMRIRQSLEKEVLHCGEALRVEIFEKQKLQAELVLLGASLKEKEQLVQEIT 660
             LN    E+                 LR E+ +K++L+ E+  LG  +KEKE LVQ   
Sbjct: 601 VELNLHVTEK--------------EGTLRSEMVDKERLKEEIHRLGCLVKEKENLVQTAE 660

Query: 661 SALEKEKGKLALAYEEVGSLKDQTNRQETLILKGHEESNITQLKLADALRKVEFLEVETC 720
           + L  E+ K+ +  +++  L+ Q  RQET I    E  ++     A  L KV+  E +  
Sbjct: 661 NNLATERKKIEVVSQQINDLQSQVERQETEIQDKIEALSVVS---ARELEKVKGYETKIS 720

Query: 721 KSKQNLEQAMMELSKVDEERRMLVARLLESQKTELLVEEKEKES-RKQMESVIILVQELL 780
             ++ LE A   L ++ +E+R    +L E+        + EKE+ +KQ+ S+ ++V   L
Sbjct: 721 SLREELELARESLKEMKDEKRKTEEKLSET--------KAEKETLKKQLVSLDLVVPPQL 780

Query: 781 KEVFD-FEHRAIDYISRNNKRLESLSSQTNSLIQNACMLKRAGLIYKQRHEKRCSDLQKA 840
            + FD  E    +   + N RL+++ SQ + L      +K     YKQR EK+C DL+KA
Sbjct: 781 IKGFDILEGLIAEKTQKTNSRLKNMQSQLSDLSHQINEVKGKASTYKQRLEKKCCDLKKA 820

Query: 841 EAEVDLLGDEVDALLRLLEKMYIALDHYSPILQHYPGIVDILKLVKRELSGDT 873
           EAEVDLLGDEV+ LL LLEK+YIALDHYSPIL+HYPGI++IL+LV+RELSG++
Sbjct: 841 EAEVDLLGDEVETLLDLLEKIYIALDHYSPILKHYPGIIEILRLVRRELSGES 820

BLAST of MC01g1612 vs. TAIR 10
Match: AT2G34730.2 (myosin heavy chain-related )

HSP 1 Score: 398.7 bits (1023), Expect = 1.2e-110
Identity = 310/893 (34.71%), Postives = 476/893 (53.30%), Query Frame = 0

Query: 1   MDANATPEENGNMQ---------LSASDKQLEDLGDDIQILEDLDSYLEDIDDRLTISRM 60
           M+      ENG+++         L  +    E+   D+  LEDLDSY EDI+DRLTISR+
Sbjct: 1   MEEEVVKSENGSLEFHDDTLSSSLQVNGVLKENENPDVDFLEDLDSYWEDINDRLTISRV 60

Query: 61  VSDAVIKGMVNAISEEANEKITRKESEVSELREILKCYHLGSEKNKSLVSPLRSNKSKST 120
           VSD++I+GMV AI  +A EKI +K+ E+S++RE L  YH+GSE+N+S  S L        
Sbjct: 61  VSDSIIRGMVTAIESDAAEKIAQKDLELSKIRETLLLYHVGSEENESSESRL-------- 120

Query: 121 KCDRNEGISGALFEHDEM-RDSVCNLKNAAKENFKKLRKEIDKIRGCSSIRKINSGSELV 180
                         HDE+ + S  +LK  A++    L +E+  +R    I    SG+ + 
Sbjct: 121 -------------IHDELTQGSSSSLKKKARKQLLMLVEELTNLREYIHIN--GSGATVD 180

Query: 181 GLSGILQEKASSRCVDVDKIVDDLQDNLDTFYNQVEDIVQVSKASLGQWQDEKEFLADIE 240
              G+      +R   VDK++D L+  L+T   +  D+   S      WQ E +F  +IE
Sbjct: 181 DSLGLDSSPHETRSKTVDKMLDSLKSILETVLKRKNDMELPS-----SWQQEHDFQKEIE 240

Query: 241 GMVIRNYIWSVQQELEEKLWDQNAKIFSNERKIWVQKIKEISSLRQELDIISKSL----- 300
             V+ + + S++ E E++L DQ A+ F   R + +  IKEI+ LRQEL+ I KS      
Sbjct: 241 SAVVTSVLRSLKDEYEQRLLDQKAE-FGGNRSLILGNIKEITGLRQELEAIRKSFLDHEN 300

Query: 301 SPEVGHLFSYSSMDSDHSHRKLLSNHMAPSTGNGKHELSKTNL-PENVDPARLKHLNRDE 360
             E G +     ++  H       N ++    NGKHE S T L PE+ +   L+H++ DE
Sbjct: 301 GDEAGEVGDRKRVEQLHRKMSGSLNSVSSVWENGKHEESSTGLIPEHNE--TLRHMSPDE 360

Query: 361 LINHFNTEMTKMSRNHESLVHEITEENFTLKRELLKEKEKSSLK---KDKEFDILRKKIP 420
           +INHF  EM KM R+H+  + E+TE+ FT KR+ L   E+ S     KDKE   L+KKIP
Sbjct: 361 MINHFKIEMNKMKRDHDYKIQELTEQCFTFKRKYLNLTERGSFSFVGKDKELGALKKKIP 420

Query: 421 DIILKLDDILMENEKLSVSAANDENLGTMRNRLESLIAENRHLKDSLEEKKKEVKCLSLQ 480
            +I KLD ILME+EK      ND  L   + +L+SL+ ENR LKDSL             
Sbjct: 421 FVISKLDKILMEDEKFVSEGKNDAGL---KRQLDSLLLENRQLKDSL------------- 480

Query: 481 ISSSSEKMSQQSLRHAKSLNTIEKIKCEMQDAKFEASIGEDIVKCFLRETMDTIRCVNEE 540
            S ++EKMSQ S   A     I K++ +++D++ EASI ED+  CF+ E +  I+C  +E
Sbjct: 481 -SDAAEKMSQLSQAEADHQELIRKLETDVEDSRNEASIYEDVYGCFVTEFVGQIKCTKQE 540

Query: 541 SALRYDIMQGIYETIFQGASFRGELASTSEDEHLDEESIIMQGILGVVLQESWKEAEEKM 600
           + L + +++  YE + +  + +    S  + E    +S++M+    V+ +E+ KEA +K+
Sbjct: 541 TDLEHSMLREAYELLLEDLARKEARKSKEDFEDSCVKSVMMEECCSVIYKEAVKEAHKKI 600

Query: 601 TSLNNRYIEEMRIRQSLEKEVLHCGEALRVEIFEKQKLQAELVLLGASLKEKEQLVQEIT 660
             LN    E+                 LR E+ +K++L+ E+  LG  +KEKE LVQ   
Sbjct: 601 VELNLHVTEK--------------EGTLRSEMVDKERLKEEIHRLGCLVKEKENLVQTAE 660

Query: 661 SALEKEKGKLALAYEEVGSLKDQTNRQETLILKGHEESNITQLKLADALRKVEFLEVETC 720
           + L  E+ K+ +  +++  L+ Q  RQET I    E  ++     A  L KV+  E +  
Sbjct: 661 NNLATERKKIEVVSQQINDLQSQVERQETEIQDKIEALSVVS---ARELEKVKGYETKIS 720

Query: 721 KSKQNLEQAMMELSKVDEERRMLVARLLESQKTELLVEEKEKES-RKQMESVIILVQELL 780
             ++ LE A   L ++ +E+R    +L E+        + EKE+ +KQ+ S+ ++V   L
Sbjct: 721 SLREELELARESLKEMKDEKRKTEEKLSET--------KAEKETLKKQLVSLDLVVPPQL 780

Query: 781 KEVFD-FEHRAIDYISRNNKRLESLSSQTNSLIQNACMLKRAGLIYKQRHEKRCSDLQKA 840
            + FD  E    +   + N RL+++ SQ + L      +K     YKQR EK+C   +  
Sbjct: 781 IKGFDILEGLIAEKTQKTNSRLKNMQSQLSDLSHQINEVKGKASTYKQRLEKKCCVCELC 820

Query: 841 EAEVDLLGDEVDALLRLLEKMYIALDHYSPILQHYPGIVDILKLVKRELSGDT 873
                +    V+ LL LLEK+YIALDHYSPIL+HYPGI++IL+LV+RELSG++
Sbjct: 841 PYPYLVEELTVETLLDLLEKIYIALDHYSPILKHYPGIIEILRLVRRELSGES 820

BLAST of MC01g1612 vs. TAIR 10
Match: AT5G14990.1 (BEST Arabidopsis thaliana protein match is: myosin heavy chain-related (TAIR:AT2G34730.1); Has 8284 Blast hits to 6001 proteins in 578 species: Archae - 107; Bacteria - 678; Metazoa - 3983; Fungi - 607; Plants - 315; Viruses - 16; Other Eukaryotes - 2578 (source: NCBI BLink). )

HSP 1 Score: 53.9 bits (128), Expect = 7.5e-07
Identity = 60/211 (28.44%), Postives = 103/211 (48.82%), Query Frame = 0

Query: 672 ILKGHEESNITQLKLADALR---KVEFLEVET----CKSKQNLEQAMMELSKVDEERR-- 731
           +LKG +   IT  + A+      K E +E E     C    N E+   E+   DEE R  
Sbjct: 452 LLKGLKADEITNNRKAEEEEEEIKSEKIESEVKCMDCLENLNREKD-YEILLEDEEFRQE 511

Query: 732 ---MLVARLLESQKTELLVEEKEKESRKQM--ESVIILVQELLKEVFDFEHRAIDYISRN 791
              ++V  LL      +   EK + + K++  E V     E+     DF+ +  + +   
Sbjct: 512 LSWIIVTELLREVSETVENHEKIEANNKRVIEEEVNRACLEISLLYDDFDFKIQEKLKMV 571

Query: 792 NKRLESLSSQTNSLIQNACMLKRAGLIYKQRHEKRCSDLQKAEAEVDLLGDEVDALLRLL 851
             RL++L  + +S +     L++   +Y+     R  +L+KAE EVDLLGD+VD+L++LL
Sbjct: 572 TFRLQNLEIKIDSTMDFIAELRQRESVYRTAFVLRSENLRKAETEVDLLGDQVDSLVKLL 631

Query: 852 EKMYIALDHYSPILQHYPGIVDILKLVKREL 869
           +K       +  +L +   I++I K++K+EL
Sbjct: 632 QKTLWTFHQHPLLLCNNSDILEISKMIKKEL 661