MC01g1487 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC01g1487
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	GDSL esterase/lipase
Location	MC01: 19273351 .. 19277500 (+)
RNA-Seq Expression	MC01g1487
Synteny	MC01g1487

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

AACCCAATAAAAATGTTTTATTTTAGTTTTTTTTTTAGTATAACGAAGTAGAAATAGGAGATGTTTGAACGAAGAAATAAGAAATCTAACGACAAATTGTTGCTTTGTTAACTGTTGCTGTCACGAGGTTTGACGTTTGAAGTTTGACCTCGTTTTCTCAAACCACATGTGAGTGCTGCTGTTTATGACTACTAGCGCTATCGTATACAGAATTTCTTCCCTTTTCCCCATCTTTGAATTGAGAATGTTCTATTTACAATTGTAATTCAAAACTGCCATCGGCAACTACGTGTGGCATAAATGGCGATGGTTTTTGTATCCATCGTTTAGGCCACAACTTAGTCTTCGATTTCGCATTGGCCCATTAACTAGGCCAACTTAAAGGAGAACTCGATGATGTTGATTGGATTGGATTTTTCTTTTGTTGCATGTTGTTGAGCAATTGATGGGGGCAACTGGCAAGAGATTGTACTTCAATAACCGATCAAAGAAATGCCCTAATTTGTGGGGAATTTCCTTAGATTTGTTGAGGCAAAGCGTCGAACAGTTGGGCGACAATTCCGACCCAGTTTTCAGGGTTTTGGGTGCGAATCCTTTATACTCCACAATTGTAAAGCTCGGGTACTCGAAAAAGTATTGTAAAGTTTGGATATAGGTACCCGGAGTAAGATAATCAACCACTTCATCACGAAAAGTCATAAGCAGCTAATTAATGACACAATTACTGCTCTAATGTAGGTGCACCGTGCCGGTGCAGTAGATGATGGTACTCCGTTAGGTTTCGGCTGCTTCTGTGCAAATAAGCAACCGATTTGAAAGATGAGGCCGAAGATTTATCTTTTCGGCGATTCAATTACAGAATTTTCTTTTGCTGATGGGGGCTGGGGCGCCGCTCTTGCCCATCATTTCTCTCGCAGGGTAAATCCTGGATCATTCCACTCGAGCCACTAACAAGAACAACTCCCAACCTTATTTTCCTCATTTTTGTGAATTGCTGATCCTGAATCGGCGAGATTTTGGGAGCTCTATACGAGGTGTATTTTTCAATTTTCATCTGTTTGATTGTGATCAGGTCGATGTGGTGGTGAGAGGGTACAGCGGCTACAATACGAGATGGGCGCTCAAGGTGCTGGAACGAGTCTTCCCTGAATATTGCGACGGCGGAGGCGACGGTGCGCCGCTTGCCGTGACGGTGTTCTTTGGAGCTAATGACGCTTGCCTTCCAGATGGATATGGCGCCTTCCAACACGTGCCCCTCCATGAATACAAGCAAAACCTCAATTCCATCGTCTCCTTTTTCAAGGTATCCCCTCGCACCACAATGCTGCCATATTCTTGGTCAATAATTTCTGAAAAGGGAATATACCCCATATCCAAAGTTTTAGATTTATATTGTGCTTGAATGTGAACATAGATCAAGTCAGTTAGAAGTGGAGAAGATTTTATATACATAAGTAAGGATAACGTCTTTATTAGTACAAGACTTTTTTTGGTAGAATCTAAAAGTAAAACGATGTGACGATATGACTAGCAGCTTTTAACTCAGTTTTTGGAGGAGACACCCTTTTGGTTGGCATGATAATTGGGGATTACTTTGAACTGGGTTTTGTTTTTATGGTTAGTTGGATTCCCTTATCGAAATGCATATGTAAAGGACTGTTAACTGTATACTTTGCAAGAAAATCCTTCAGAGAAAAGCAAATTTACCTTTATACAAATCTTGCTGTGGGAACTTTTTGTTATTTGCATAAAAGTTACTGGTGGTGGTGCCTACAAACAACATAAGAACACAATGATTTTTTCTGTTTCACTTTTCCTTTTTTTCTACTTGCAATTCCAAAAGAGTCTGAATTTTGCTTCTATTTGATTCTCAGAAGAAATGGCCAGCAACCCGAATTCTACTTATCACTCCTCCTCCTATTGATGAAGAAGGGCGCCTACTGTATGCTTATCGCTACTATTGTCTTGGTTTAGCTCAAATTATATTGCGCTCTTAATCTAAGTCAAGTTTTTAAATGATGTGTCTGATAATTGGAAATGAAAACAATTCTCTTAAAACAATTTCTGCAGAAACCCTTATATTAAAAATCCATCAAATGAGCCTGAGAGGACTAACAATGCAGCTGGTGATTATGCCAAGGCTTGCATTGCTGTTGCTACAGAATGTGGGGTTCCTGTGATTGATATTTGGACTAAGATGCAGCAAATTCCTGGCTGGGAAAAGGCTTGTTTAAGGTACTTCTGTTTTCATTCACGTGCTTGCTTTGATATTTTTACAAAGGCAAATGGTTCGAATGATCTGTTGGCATTGTTGCAGTATTATTTGGAAGTTTTGTTGAACTGATCATGTATGCCTGGAATTTACAAGATATGGAATTATTTCAGGACATTCAAATTTGTAAAACTTGGACAAATGTACATTTTTGTCACATATGTGGCCACAGAATTAGGTGTGATTGCTTAATCTTAAGCTGCCCCTTTTACCTCTCTGTGGCGGACTAGGCCAGTCAAAGTTTCCATTTCCTAATGATTTTACTCTGTACGATGTTAAGGATCTAGCAATAGATTGAAAAAGCTTTAAAAGAGAGCTATCATTCAGCATTATAAAACAATCCAAAATTTCAAAGCTTTCAGAATTAGTAGTGATTGTACATAGTATCAGAGATCTTGAGTTCATTCTATAACGTTATTTCCTCCCCAGTTAATATTGATAATCACTTGTTAGGCCTTCTCCAATTCCCAAGCCCACAAGTGAGGGGATATTAAAATATCTATAAACGGTACTTATTAGGTTAAGATTTTGGGTTCAATGGTTGGTAGTCTAATAACTCTTAGCACCACCCTCACTCCCCCCACCCTCACTCCCTTCCCCCATGGCAATCCCCTGTACAAATATTCCTTTTGTCGAACAAACTATTGAACCCGATAATGCAAATTACAAGTTACTCGTCTCACTTCCGGCTGATTGCTTTTAGCTGACGATCCCCTGCCTGATTTCCCCTCAACTTTCCTTGAATAGGTGCACCAAATTGGAGGCCTGTCATACAGACAGCAAGTCTAAGCCAACTCAACCAATTGAGCTGCTCTGAGGCTGAGCTTATATGTAACATGTTTGTTGCCCATGGTTCTTCTGGTGTTGTTGGGTGCCTTTTCTTTAGTTATCTAGAAATTGCTTTATAAGATCACGTTTATCATAAATGTGCATTTATTTATACCATCCATGGCTCGTGTTTTTCCTCATCTTTAGTGTTCCCAAGTTAGAGACTCTTAGTAACCAAACTCAAATGGAAACAACTTTAAAAAATTCACTATCCAAAAAGATTTCTGTATTAATAAGTATCTAGATTTCAAAGAACGACTTATGAGAAACTTTTTTGCTCCTCCTTACTTCTCCTTGCTCTTGATATGGGAATCTGTAGTATTTTCATTCTTGAAAGAGAAATCTGTCCATTTGTTTGCAGTGATGGGCTGCACCTGACTGAAAATGGCAATAGGGTCGTTTTCAAGGAAGTCATCCAGAAGCTGAGAGAAGAAGGATTGAGTTCAGAAACGCTACCTGCTGATCTCCCACTTTTTTCAGAAATAGACTTCAATGATCCTCTCAAAGCTTTTGAAAAATTATGATACCATCTTCACTATTGGTGCTGCCCAACCTGGGTTGGTCGAAATCTCAAGTTAATCAGTTGGCTCAAACTGAGGATGTTCTTAAAGAGTGGCTCAATTTCTTATCACAAACCTATAAGACCGAAGAATGAAAAAATTCATCTACAAATGATTTCCATTGTTTAGAATAATAATAAACTTATGCTGGATCATGCCCGTTTGCAAGTATAAATTGTATGGATCAATTAGATTGCATGTTATGTATGGAATTTCTTGGAGTTGTGACCTTTAGGTCAACAAATGAGATAGATCTTAACGACTCTGTACTTTAAAATTGCGTATTCTTCATTAGAGGTTTCTGATATAGAAATTGTTGGATGTGAAATTTATCCCTTCCGACGACTCAATGGCAGATTTTTTCGATCCGATTTATTCCGAGAAATTAGTTACCATTTTCAGCATAGACGGCGGCGCTCGAATACGAAGTTACGTCTTTTTATGTCCTTTTAACTTGTTAATATCATTCCTCGATCCTTCACTGTAAATT

mRNA sequence

AACCCAATAAAAATGTTTTATTTTAGTTTTTTTTTTAGTATAACGAAGTAGAAATAGGAGATGTTTGAACGAAGAAATAAGAAATCTAACGACAAATTGTTGCTTTGTTAACTGTTGCTGTCACGAGGTTTGACGTTTGAAGTTTGACCTCGTTTTCTCAAACCACATGTGAGTGCTGCTGTTTATGACTACTAGCGCTATCGTATACAGAATTTCTTCCCTTTTCCCCATCTTTGAATTGAGAATGTTCTATTTACAATTGTAATTCAAAACTGCCATCGGCAACTACGTGTGGCATAAATGGCGATGGTTTTTGTATCCATCGTTTAGGCCACAACTTAGTCTTCGATTTCGCATTGGCCCATTAACTAGGCCAACTTAAAGGAGAACTCGATGATGTTGATTGGATTGGATTTTTCTTTTGTTGCATGTTGTTGAGCAATTGATGGGGGCAACTGGCAAGAGATTGTACTTCAATAACCGATCAAAGAAATGCCCTAATTTGTGGGGAATTTCCTTAGATTTGTTGAGGCAAAGCGTCGAACAGTTGGGCGACAATTCCGACCCAGTTTTCAGGGTTTTGGGTGCACCGTGCCGGTGCAGTAGATGATGGTACTCCGTTAGGTTTCGGCTGCTTCTGTGCAAATAAGCAACCGATTTGAAAGATGAGGCCGAAGATTTATCTTTTCGGCGATTCAATTACAGAATTTTCTTTTGCTGATGGGGGCTGGGGCGCCGCTCTTGCCCATCATTTCTCTCGCAGGGTCGATGTGGTGGTGAGAGGGTACAGCGGCTACAATACGAGATGGGCGCTCAAGGTGCTGGAACGAGTCTTCCCTGAATATTGCGACGGCGGAGGCGACGGTGCGCCGCTTGCCGTGACGGTGTTCTTTGGAGCTAATGACGCTTGCCTTCCAGATGGATATGGCGCCTTCCAACACGTGCCCCTCCATGAATACAAGCAAAACCTCAATTCCATCGTCTCCTTTTTCAAGAAGAAATGGCCAGCAACCCGAATTCTACTTATCACTCCTCCTCCTATTGATGAAGAAGGGCGCCTACTAAACCCTTATATTAAAAATCCATCAAATGAGCCTGAGAGGACTAACAATGCAGCTGGTGATTATGCCAAGGCTTGCATTGCTGTTGCTACAGAATGTGGGGTTCCTGTGATTGATATTTGGACTAAGATGCAGCAAATTCCTGGCTGGGAAAAGGCTTGTTTAAGTGATGGGCTGCACCTGACTGAAAATGGCAATAGGGTCGTTTTCAAGGAAGTCATCCAGAAGCTGAGAGAAGAAGGATTGAGTTCAGAAACGCTACCTGCTGATCTCCCACTTTTTTCAGAAATAGACTTCAATGATCCTCTCAAAGCTTTTGAAAAATTATGATACCATCTTCACTATTGGTGCTGCCCAACCTGGGTTGGTCGAAATCTCAAGTTAATCAGTTGGCTCAAACTGAGGATGTTCTTAAAGAGTGGCTCAATTTCTTATCACAAACCTATAAGACCGAAGAATGAAAAAATTCATCTACAAATGATTTCCATTGTTTAGAATAATAATAAACTTATGCTGGATCATGCCCGTTTGCAAGTATAAATTGTATGGATCAATTAGATTGCATGTTATGTATGGAATTTCTTGGAGTTGTGACCTTTAGGTCAACAAATGAGATAGATCTTAACGACTCTGTACTTTAAAATTGCGTATTCTTCATTAGAGGTTTCTGATATAGAAATTGTTGGATGTGAAATTTATCCCTTCCGACGACTCAATGGCAGATTTTTTCGATCCGATTTATTCCGAGAAATTAGTTACCATTTTCAGCATAGACGGCGGCGCTCGAATACGAAGTTACGTCTTTTTATGTCCTTTTAACTTGTTAATATCATTCCTCGATCCTTCACTGTAAATT

Coding sequence (CDS)

ATGAGGCCGAAGATTTATCTTTTCGGCGATTCAATTACAGAATTTTCTTTTGCTGATGGGGGCTGGGGCGCCGCTCTTGCCCATCATTTCTCTCGCAGGGTCGATGTGGTGGTGAGAGGGTACAGCGGCTACAATACGAGATGGGCGCTCAAGGTGCTGGAACGAGTCTTCCCTGAATATTGCGACGGCGGAGGCGACGGTGCGCCGCTTGCCGTGACGGTGTTCTTTGGAGCTAATGACGCTTGCCTTCCAGATGGATATGGCGCCTTCCAACACGTGCCCCTCCATGAATACAAGCAAAACCTCAATTCCATCGTCTCCTTTTTCAAGAAGAAATGGCCAGCAACCCGAATTCTACTTATCACTCCTCCTCCTATTGATGAAGAAGGGCGCCTACTAAACCCTTATATTAAAAATCCATCAAATGAGCCTGAGAGGACTAACAATGCAGCTGGTGATTATGCCAAGGCTTGCATTGCTGTTGCTACAGAATGTGGGGTTCCTGTGATTGATATTTGGACTAAGATGCAGCAAATTCCTGGCTGGGAAAAGGCTTGTTTAAGTGATGGGCTGCACCTGACTGAAAATGGCAATAGGGTCGTTTTCAAGGAAGTCATCCAGAAGCTGAGAGAAGAAGGATTGAGTTCAGAAACGCTACCTGCTGATCTCCCACTTTTTTCAGAAATAGACTTCAATGATCCTCTCAAAGCTTTTGAAAAATTATGA

Protein sequence

MRPKIYLFGDSITEFSFADGGWGAALAHHFSRRVDVVVRGYSGYNTRWALKVLERVFPEYCDGGGDGAPLAVTVFFGANDACLPDGYGAFQHVPLHEYKQNLNSIVSFFKKKWPATRILLITPPPIDEEGRLLNPYIKNPSNEPERTNNAAGDYAKACIAVATECGVPVIDIWTKMQQIPGWEKACLSDGLHLTENGNRVVFKEVIQKLREEGLSSETLPADLPLFSEIDFNDPLKAFEKL

Homology

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BLAST of MC01g1487 vs. ExPASy Swiss-Prot
Match: Q6NMR9 (GDSL esterase/lipase At5g45920 OS=Arabidopsis thaliana OX=3702 GN=At5g45920 PE=2 SV=1)

HSP 1 Score: 342.4 bits (877), Expect = 4.1e-93
Identity = 160/240 (66.67%), Postives = 190/240 (79.17%), Query Frame = 0


Query: 1   MRPKIYLFGDSITEFSFADGGWGAALAHHFSRRVDVVVRGYSGYNTRWALKVLERVFPEY 60
           MR KI+LFGDSITE SF+DGGWGA+LA    R+ D+V+RGYSGYNTRWALKV+ERVFP  
Sbjct: 2   MRRKIFLFGDSITEESFSDGGWGASLADLLRRKADMVLRGYSGYNTRWALKVVERVFPVA 61

Query: 61  CDGGGDGAPLAVTVFFGANDACLPDGYGAFQHVPLHEYKQNLNSIVSFFKKKWPATRILL 120
            + GGD +P AVTVFFGANDACLP+    FQHVPLHEYKQNL SIVSF K +WP T I+L
Sbjct: 62  EEDGGD-SPAAVTVFFGANDACLPERCSGFQHVPLHEYKQNLRSIVSFLKNRWPQTAIIL 121

Query: 121 ITPPPIDEEGRLLNPYIKNPSNEPERTNNAAGDYAKACIAVATECGVPVIDIWTKMQQIP 180
           ITPPPIDEE RL  PYI+N +  PERTN  AG YAKACIAVA EC + V D+W+KMQQIP
Sbjct: 122 ITPPPIDEEARLRYPYIENTTGLPERTNEVAGLYAKACIAVAEECQISVTDLWSKMQQIP 181

Query: 181 GWEKACLSDGLHLTENGNRVVFKEVIQKLREEGLSSETLPADLPLFSEIDFNDPLKAFEK 240
            W+  CL DGLHL+  GN+VVF+EV +KL+EEG+ +E L  DLPL  ++D  DPLK+F++
Sbjct: 182 NWQTECLWDGLHLSRVGNKVVFEEVAKKLKEEGIGAEDLAVDLPLIEDVDPKDPLKSFDE 240

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BLAST of MC01g1487 vs. ExPASy Swiss-Prot
Match: Q9FM04 (GDSL esterase/lipase At5g62930 OS=Arabidopsis thaliana OX=3702 GN=At5g62930 PE=2 SV=3)

HSP 1 Score: 251.1 bits (640), Expect = 1.2e-65
Identity = 128/243 (52.67%), Postives = 155/243 (63.79%), Query Frame = 0


Query: 1   MRPKIYLFGDSITEFSFADGGWGAALAHHFSRRVDVVVRGYSGYNTRWALKVLERVFPEY 60
           MRP+I LFGDSIT  SF  GGWG+ALA  +SR+ DVVVRGY GYNTRWAL +L  +FP  
Sbjct: 1   MRPEIVLFGDSITAQSFRSGGWGSALADAYSRKADVVVRGYGGYNTRWALFLLHHIFPL- 60

Query: 61  CDGGGDGAPLAVTVFFGANDACLPDGYGAFQHVPLHEYKQNLNSIVSFFKKKWPATRILL 120
              G    P+A T+FFGANDA L       QHVP+ EY  N+  IV   KK  P   I+L
Sbjct: 61  ---GSSSPPVATTIFFGANDAALKGRTSDRQHVPVEEYTDNVRKIVQHLKKCSPTMLIVL 120

Query: 121 ITPPPIDEEGR---LLNPYIKNPSNEPERTNNAAGDYAKACIAVATECGVPVIDIWTKMQ 180
           ITPPPIDE GR     + Y +    EPERTN   G YA+ C+A+A E G+  +++W+KMQ
Sbjct: 121 ITPPPIDEAGRQSYAESIYGEKAMKEPERTNETTGVYAQHCVALAEELGLRCVNLWSKMQ 180

Query: 181 QIPGWEKACLSDGLHLTENGNRVVFKEVIQKLREEGLSSETLPADLPLFSEIDFNDPLKA 240
           +   W+K  LSDGLHLT  GN VVF EV +  RE  LS E +P D P  S ID  +P KA
Sbjct: 181 ETNDWQKKYLSDGLHLTPEGNGVVFDEVSRVFREAWLSPEEMPFDFPHHSHIDGKNPSKA 239

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BLAST of MC01g1487 vs. ExPASy Swiss-Prot
Match: Q711G3 (Isoamyl acetate-hydrolyzing esterase 1 homolog OS=Rattus norvegicus OX=10116 GN=Iah1 PE=2 SV=2)

HSP 1 Score: 183.0 bits (463), Expect = 4.1e-45
Identity = 103/233 (44.21%), Postives = 140/233 (60.09%), Query Frame = 0


Query: 3   PKIYLFGDSITEFSFADGGWGAALAHHFSRRVDVVVRGYSGYNTRWALKVLERVFPEYCD 62
           P++ LFGDSIT+FSF  GGWG  LA    R+ DV+ RG+SGYNTRWA  +L R+  +   
Sbjct: 16  PRVLLFGDSITQFSFQQGGWGTLLADRLVRKCDVLNRGFSGYNTRWAKIILPRIIRK--- 75

Query: 63  GGGDGAPLAVTVFFGANDACLPDGYGAFQHVPLHEYKQNLNSIVSFFKK-KWPATRILLI 122
           G G   P+AVT+FFGAND+ L D     QHVPL EY  NL  +V + +    P  R++LI
Sbjct: 76  GAGLENPVAVTIFFGANDSTLKD-ENPKQHVPLDEYSANLRDMVQYLRSVDIPKERVILI 135

Query: 123 TPPPIDEEGRLLNPYIKNPSNEPERTNNAAGDYAKACIAVATECGVPVIDIWTKMQQIPG 182
           TPPP+ E        +K    +  R N A G+YAKAC+ VA +CG  V+D+WT MQ+   
Sbjct: 136 TPPPLCEAAWEKECILK--GCKLNRLNVAVGEYAKACLQVARDCGTDVLDLWTLMQKDNQ 195

Query: 183 WEKACLSDGLHLTENGNRVVFKEVIQKLREEGLSSETLPADLPLFSEIDFNDP 235
              + LSDGLHL+  GN  +F   +  L ++ +SS  LP  LP + +++   P
Sbjct: 196 DFSSYLSDGLHLSPLGNEFLFFH-LWPLLDKKVSS--LPRLLPDWKDVEETKP 239

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BLAST of MC01g1487 vs. ExPASy Swiss-Prot
Match: Q9DB29 (Isoamyl acetate-hydrolyzing esterase 1 homolog OS=Mus musculus OX=10090 GN=Iah1 PE=1 SV=1)

HSP 1 Score: 181.0 bits (458), Expect = 1.6e-44
Identity = 99/233 (42.49%), Postives = 138/233 (59.23%), Query Frame = 0


Query: 3   PKIYLFGDSITEFSFADGGWGAALAHHFSRRVDVVVRGYSGYNTRWALKVLERVFPEYCD 62
           P++ LFGDSIT+FSF  GGWG+ LA    R+ DV+ RG+SGYNTRWA  +L R+  +   
Sbjct: 16  PRVLLFGDSITQFSFQQGGWGSLLADRLVRKCDVLNRGFSGYNTRWAKIILPRLIRK--- 75

Query: 63  GGGDGAPLAVTVFFGANDACLPDGYGAFQHVPLHEYKQNLNSIVSFFKK-KWPATRILLI 122
           G G   P+AVT+FFGAND+ L D     QHVPL EY  NL  +V + +    P  R++LI
Sbjct: 76  GPGMENPVAVTIFFGANDSSLKD-ENPKQHVPLDEYSANLRDMVQYLRSVDVPRERVILI 135

Query: 123 TPPPIDEEGRLLNPYIKNPSNEPERTNNAAGDYAKACIAVATECGVPVIDIWTKMQQIPG 182
           TPPP+ E        +K    +  R N+  G+YA AC+ VA +CG  V+D+WT MQ+   
Sbjct: 136 TPPPLCEAAWEKECVLK--GCKLNRLNSVVGEYANACLQVARDCGTDVLDLWTLMQKDSQ 195

Query: 183 WEKACLSDGLHLTENGNRVVFKEVIQKLREEGLSSETLPADLPLFSEIDFNDP 235
              + LSDGLHL+  GN  +F  +   L ++     +LP  LP + +++   P
Sbjct: 196 DFSSYLSDGLHLSPMGNEFLFLNLCPLLDKK---VSSLPWLLPYWKDVEEAKP 239

[Close]

BLAST of MC01g1487 vs. ExPASy Swiss-Prot
Match: Q3SZ16 (Isoamyl acetate-hydrolyzing esterase 1 homolog OS=Bos taurus OX=9913 GN=IAH1 PE=2 SV=1)

HSP 1 Score: 180.6 bits (457), Expect = 2.1e-44
Identity = 101/228 (44.30%), Postives = 139/228 (60.96%), Query Frame = 0


Query: 3   PKIYLFGDSITEFSFADGGWGAALAHHFSRRVDVVVRGYSGYNTRWALKVLERVFPEYCD 62
           P++ LFGDSIT+FSF  GGWGA+LA    R+ DV+ RG+SGYNTRWA  +L R+  +   
Sbjct: 16  PRVLLFGDSITQFSFQQGGWGASLADMLVRKCDVLNRGFSGYNTRWAKIILPRLVRK--- 75

Query: 63  GGGDGAPLAVTVFFGANDACLPDGYGAFQHVPLHEYKQNLNSIVSFFKK-KWPATRILLI 122
           G G  +P+AVT+FFGAND+ L D     QHVPL E+  NL S+V + +    P  R++LI
Sbjct: 76  GSGLDSPVAVTIFFGANDSALKD-ENPKQHVPLEEFVANLRSMVRYLRSVDVPEGRLILI 135

Query: 123 TPPPIDEEGRLLNPYIKNPSNEPERTNNAAGDYAKACIAVATECGVPVIDIWTKMQQIPG 182
           TPPP+ E              +  R N+  G+YA+AC+ VA +CG   +D+W+ MQ+   
Sbjct: 136 TPPPLCEAAWAQE--CLQQGCKLNRLNSVVGEYARACLQVAQDCGADALDLWSLMQKDGQ 195

Query: 183 WEKACLSDGLHLTENGNRVVFKEVIQKLREEGLSSETLPADLPLFSEI 230
              + LSDGLHL+  GN  VF   +  L E+ +SS  LP  LP + +I
Sbjct: 196 DFSSYLSDGLHLSPKGNEFVFSH-LWPLIEKKVSS--LPFLLPYWRDI 234

[Close]

BLAST of MC01g1487 vs. NCBI nr
Match: XP_022142319.1 (GDSL esterase/lipase At5g45920 isoform X1 [Momordica charantia] >XP_022142320.1 GDSL esterase/lipase At5g45920 isoform X1 [Momordica charantia] >XP_022142321.1 GDSL esterase/lipase At5g45920 isoform X1 [Momordica charantia])

HSP 1 Score: 508 bits (1308), Expect = 6.11e-182
Identity = 241/241 (100.00%), Postives = 241/241 (100.00%), Query Frame = 0


Query: 1   MRPKIYLFGDSITEFSFADGGWGAALAHHFSRRVDVVVRGYSGYNTRWALKVLERVFPEY 60
           MRPKIYLFGDSITEFSFADGGWGAALAHHFSRRVDVVVRGYSGYNTRWALKVLERVFPEY
Sbjct: 1   MRPKIYLFGDSITEFSFADGGWGAALAHHFSRRVDVVVRGYSGYNTRWALKVLERVFPEY 60

Query: 61  CDGGGDGAPLAVTVFFGANDACLPDGYGAFQHVPLHEYKQNLNSIVSFFKKKWPATRILL 120
           CDGGGDGAPLAVTVFFGANDACLPDGYGAFQHVPLHEYKQNLNSIVSFFKKKWPATRILL
Sbjct: 61  CDGGGDGAPLAVTVFFGANDACLPDGYGAFQHVPLHEYKQNLNSIVSFFKKKWPATRILL 120

Query: 121 ITPPPIDEEGRLLNPYIKNPSNEPERTNNAAGDYAKACIAVATECGVPVIDIWTKMQQIP 180
           ITPPPIDEEGRLLNPYIKNPSNEPERTNNAAGDYAKACIAVATECGVPVIDIWTKMQQIP
Sbjct: 121 ITPPPIDEEGRLLNPYIKNPSNEPERTNNAAGDYAKACIAVATECGVPVIDIWTKMQQIP 180

Query: 181 GWEKACLSDGLHLTENGNRVVFKEVIQKLREEGLSSETLPADLPLFSEIDFNDPLKAFEK 240
           GWEKACLSDGLHLTENGNRVVFKEVIQKLREEGLSSETLPADLPLFSEIDFNDPLKAFEK
Sbjct: 181 GWEKACLSDGLHLTENGNRVVFKEVIQKLREEGLSSETLPADLPLFSEIDFNDPLKAFEK 240

[Close]

BLAST of MC01g1487 vs. NCBI nr
Match: XP_022978753.1 (GDSL esterase/lipase At5g45920 [Cucurbita maxima])

HSP 1 Score: 432 bits (1112), Expect = 4.83e-152
Identity = 205/241 (85.06%), Postives = 219/241 (90.87%), Query Frame = 0


Query: 1   MRPKIYLFGDSITEFSFADGGWGAALAHHFSRRVDVVVRGYSGYNTRWALKVLERVFPEY 60
           MRP+IYLFGDSITEFSFADGGWGAALA+HFSRR DVVVRGYSGYNTRWALKV+E VFP  
Sbjct: 1   MRPRIYLFGDSITEFSFADGGWGAALANHFSRRADVVVRGYSGYNTRWALKVIEHVFPAS 60

Query: 61  CDGGGDGAPLAVTVFFGANDACLPDGYGAFQHVPLHEYKQNLNSIVSFFKKKWPATRILL 120
            + GG G PLA+TVFFGANDACLPD YGAFQHVPL EYKQNL SIVSF KK+WPATRILL
Sbjct: 61  EEHGGAGDPLALTVFFGANDACLPDRYGAFQHVPLDEYKQNLISIVSFLKKQWPATRILL 120

Query: 121 ITPPPIDEEGRLLNPYIKNPSNEPERTNNAAGDYAKACIAVATECGVPVIDIWTKMQQIP 180
           ITPPPIDEEGRL NPYI NP NEPERTNNAAGDYAKACIAVA ECGV VIDIWTKMQQ+P
Sbjct: 121 ITPPPIDEEGRLQNPYIVNPLNEPERTNNAAGDYAKACIAVAAECGVSVIDIWTKMQQLP 180

Query: 181 GWEKACLSDGLHLTENGNRVVFKEVIQKLREEGLSSETLPADLPLFSEIDFNDPLKAFEK 240
           GWEKACLSDGLHL+ NGN +VF+EV+QKL+EEGLS E LPADLPL SEID+NDPLKAFE+
Sbjct: 181 GWEKACLSDGLHLSRNGNTIVFEEVVQKLKEEGLSPEMLPADLPLLSEIDYNDPLKAFEE 240

[Close]

BLAST of MC01g1487 vs. NCBI nr
Match: XP_022925816.1 (GDSL esterase/lipase At5g45920 [Cucurbita moschata] >KAG7034633.1 GDSL esterase/lipase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 432 bits (1111), Expect = 6.86e-152
Identity = 204/241 (84.65%), Postives = 220/241 (91.29%), Query Frame = 0


Query: 1   MRPKIYLFGDSITEFSFADGGWGAALAHHFSRRVDVVVRGYSGYNTRWALKVLERVFPEY 60
           MRP+IYLFGDSITEFSFADGGWGAALA+HFSRR DVVVRGYSGYNTRWALKVLERVFP  
Sbjct: 1   MRPRIYLFGDSITEFSFADGGWGAALANHFSRRADVVVRGYSGYNTRWALKVLERVFPAS 60

Query: 61  CDGGGDGAPLAVTVFFGANDACLPDGYGAFQHVPLHEYKQNLNSIVSFFKKKWPATRILL 120
            +  G G PLA+TVFFGANDACLPD YGAFQHVPL EYKQNL SIVSF KK+WPATRILL
Sbjct: 61  EEHSGAGDPLALTVFFGANDACLPDRYGAFQHVPLDEYKQNLISIVSFLKKQWPATRILL 120

Query: 121 ITPPPIDEEGRLLNPYIKNPSNEPERTNNAAGDYAKACIAVATECGVPVIDIWTKMQQIP 180
           ITPPPIDEEGRL NPY++NP NEPERTNNAAGDYAKACIAVA ECGV VIDIWTKMQQ+P
Sbjct: 121 ITPPPIDEEGRLQNPYLENPLNEPERTNNAAGDYAKACIAVAAECGVSVIDIWTKMQQLP 180

Query: 181 GWEKACLSDGLHLTENGNRVVFKEVIQKLREEGLSSETLPADLPLFSEIDFNDPLKAFEK 240
           GWEKACLSDGLHL+ NGN +VF+EV+QKL+EEGLS E LPADLPL SE+D+NDPLKAFE+
Sbjct: 181 GWEKACLSDGLHLSRNGNTIVFEEVVQKLKEEGLSPEMLPADLPLLSEMDYNDPLKAFEE 240

[Close]

BLAST of MC01g1487 vs. NCBI nr
Match: XP_038882981.1 (GDSL esterase/lipase At5g45920 [Benincasa hispida] >XP_038882982.1 GDSL esterase/lipase At5g45920 [Benincasa hispida])

HSP 1 Score: 432 bits (1111), Expect = 7.64e-152
Identity = 207/245 (84.49%), Postives = 221/245 (90.20%), Query Frame = 0


Query: 1   MRPKIYLFGDSITEFSFADGGWGAALAHHFSRRVDVVVRGYSGYNTRWALKVLERVFP-- 60
           MRPKIYLFGDSITEFSFADGGWG+ALA+HFSRR DVVVRGYSGYNTRWALKV+ERVFP  
Sbjct: 1   MRPKIYLFGDSITEFSFADGGWGSALANHFSRRADVVVRGYSGYNTRWALKVIERVFPSS 60

Query: 61  EYCDGGGDGA--PLAVTVFFGANDACLPDGYGAFQHVPLHEYKQNLNSIVSFFKKKWPAT 120
           E   G GDGA  PLA TVFFGANDACLPD YGAFQHVPLHEYKQNL SIVSFFKKKWPAT
Sbjct: 61  EEHGGSGDGAASPLAATVFFGANDACLPDRYGAFQHVPLHEYKQNLISIVSFFKKKWPAT 120

Query: 121 RILLITPPPIDEEGRLLNPYIKNPSNEPERTNNAAGDYAKACIAVATECGVPVIDIWTKM 180
           RIL+ITPPPIDEEGRL NPY+ NP NEPERTNNAAGDYAKACIAVA ECG+ VIDIWTKM
Sbjct: 121 RILIITPPPIDEEGRLQNPYVSNPLNEPERTNNAAGDYAKACIAVAAECGISVIDIWTKM 180

Query: 181 QQIPGWEKACLSDGLHLTENGNRVVFKEVIQKLREEGLSSETLPADLPLFSEIDFNDPLK 240
           QQ+PGWEKACLSDGLHLT NGN +VF+EV++KL EEGLS E LPA+LPLFSEID N+PLK
Sbjct: 181 QQVPGWEKACLSDGLHLTRNGNTIVFEEVVKKLEEEGLSPEKLPAELPLFSEIDSNNPLK 240

[Close]

BLAST of MC01g1487 vs. NCBI nr
Match: XP_023544567.1 (GDSL esterase/lipase At5g45920 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 429 bits (1104), Expect = 8.00e-151
Identity = 203/241 (84.23%), Postives = 218/241 (90.46%), Query Frame = 0


Query: 1   MRPKIYLFGDSITEFSFADGGWGAALAHHFSRRVDVVVRGYSGYNTRWALKVLERVFPEY 60
           MRP+IYLFGDSITEFSFADGGWG ALA+HFSRR DVVVRGYSGYNTRWALKVLERVFP  
Sbjct: 1   MRPRIYLFGDSITEFSFADGGWGGALANHFSRRADVVVRGYSGYNTRWALKVLERVFPAS 60

Query: 61  CDGGGDGAPLAVTVFFGANDACLPDGYGAFQHVPLHEYKQNLNSIVSFFKKKWPATRILL 120
            +  G G PLA+TVFFGANDACLPD YGAFQHVPL EYKQNL SIVSF KK+WPATRILL
Sbjct: 61  EEHSGAGDPLALTVFFGANDACLPDRYGAFQHVPLDEYKQNLISIVSFLKKQWPATRILL 120

Query: 121 ITPPPIDEEGRLLNPYIKNPSNEPERTNNAAGDYAKACIAVATECGVPVIDIWTKMQQIP 180
           ITPPPIDEEGRL NPY+ NP NEPERTNNAAGDYAKACIAVA ECGV VIDIWTKMQQ+P
Sbjct: 121 ITPPPIDEEGRLQNPYLVNPLNEPERTNNAAGDYAKACIAVAAECGVSVIDIWTKMQQLP 180

Query: 181 GWEKACLSDGLHLTENGNRVVFKEVIQKLREEGLSSETLPADLPLFSEIDFNDPLKAFEK 240
           GWEKACLSDGLHL+ NGN +VF+EV+QKL+EEGLS E LPADLPL SE+D+NDPLKAFE+
Sbjct: 181 GWEKACLSDGLHLSRNGNTIVFEEVVQKLKEEGLSPEMLPADLPLLSEMDYNDPLKAFEE 240

[Close]

BLAST of MC01g1487 vs. ExPASy TrEMBL
Match: A0A6J1CLU7 (GDSL esterase/lipase At5g45920 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111012462 PE=4 SV=1)

HSP 1 Score: 508 bits (1308), Expect = 2.96e-182
Identity = 241/241 (100.00%), Postives = 241/241 (100.00%), Query Frame = 0


Query: 1   MRPKIYLFGDSITEFSFADGGWGAALAHHFSRRVDVVVRGYSGYNTRWALKVLERVFPEY 60
           MRPKIYLFGDSITEFSFADGGWGAALAHHFSRRVDVVVRGYSGYNTRWALKVLERVFPEY
Sbjct: 1   MRPKIYLFGDSITEFSFADGGWGAALAHHFSRRVDVVVRGYSGYNTRWALKVLERVFPEY 60

Query: 61  CDGGGDGAPLAVTVFFGANDACLPDGYGAFQHVPLHEYKQNLNSIVSFFKKKWPATRILL 120
           CDGGGDGAPLAVTVFFGANDACLPDGYGAFQHVPLHEYKQNLNSIVSFFKKKWPATRILL
Sbjct: 61  CDGGGDGAPLAVTVFFGANDACLPDGYGAFQHVPLHEYKQNLNSIVSFFKKKWPATRILL 120

Query: 121 ITPPPIDEEGRLLNPYIKNPSNEPERTNNAAGDYAKACIAVATECGVPVIDIWTKMQQIP 180
           ITPPPIDEEGRLLNPYIKNPSNEPERTNNAAGDYAKACIAVATECGVPVIDIWTKMQQIP
Sbjct: 121 ITPPPIDEEGRLLNPYIKNPSNEPERTNNAAGDYAKACIAVATECGVPVIDIWTKMQQIP 180

Query: 181 GWEKACLSDGLHLTENGNRVVFKEVIQKLREEGLSSETLPADLPLFSEIDFNDPLKAFEK 240
           GWEKACLSDGLHLTENGNRVVFKEVIQKLREEGLSSETLPADLPLFSEIDFNDPLKAFEK
Sbjct: 181 GWEKACLSDGLHLTENGNRVVFKEVIQKLREEGLSSETLPADLPLFSEIDFNDPLKAFEK 240

[Close]

BLAST of MC01g1487 vs. ExPASy TrEMBL
Match: A0A6J1IU69 (GDSL esterase/lipase At5g45920 OS=Cucurbita maxima OX=3661 GN=LOC111478618 PE=3 SV=1)

HSP 1 Score: 432 bits (1112), Expect = 2.34e-152
Identity = 205/241 (85.06%), Postives = 219/241 (90.87%), Query Frame = 0


Query: 1   MRPKIYLFGDSITEFSFADGGWGAALAHHFSRRVDVVVRGYSGYNTRWALKVLERVFPEY 60
           MRP+IYLFGDSITEFSFADGGWGAALA+HFSRR DVVVRGYSGYNTRWALKV+E VFP  
Sbjct: 1   MRPRIYLFGDSITEFSFADGGWGAALANHFSRRADVVVRGYSGYNTRWALKVIEHVFPAS 60

Query: 61  CDGGGDGAPLAVTVFFGANDACLPDGYGAFQHVPLHEYKQNLNSIVSFFKKKWPATRILL 120
            + GG G PLA+TVFFGANDACLPD YGAFQHVPL EYKQNL SIVSF KK+WPATRILL
Sbjct: 61  EEHGGAGDPLALTVFFGANDACLPDRYGAFQHVPLDEYKQNLISIVSFLKKQWPATRILL 120

Query: 121 ITPPPIDEEGRLLNPYIKNPSNEPERTNNAAGDYAKACIAVATECGVPVIDIWTKMQQIP 180
           ITPPPIDEEGRL NPYI NP NEPERTNNAAGDYAKACIAVA ECGV VIDIWTKMQQ+P
Sbjct: 121 ITPPPIDEEGRLQNPYIVNPLNEPERTNNAAGDYAKACIAVAAECGVSVIDIWTKMQQLP 180

Query: 181 GWEKACLSDGLHLTENGNRVVFKEVIQKLREEGLSSETLPADLPLFSEIDFNDPLKAFEK 240
           GWEKACLSDGLHL+ NGN +VF+EV+QKL+EEGLS E LPADLPL SEID+NDPLKAFE+
Sbjct: 181 GWEKACLSDGLHLSRNGNTIVFEEVVQKLKEEGLSPEMLPADLPLLSEIDYNDPLKAFEE 240

[Close]

BLAST of MC01g1487 vs. ExPASy TrEMBL
Match: A0A6J1ED78 (GDSL esterase/lipase At5g45920 OS=Cucurbita moschata OX=3662 GN=LOC111433110 PE=3 SV=1)

HSP 1 Score: 432 bits (1111), Expect = 3.32e-152
Identity = 204/241 (84.65%), Postives = 220/241 (91.29%), Query Frame = 0


Query: 1   MRPKIYLFGDSITEFSFADGGWGAALAHHFSRRVDVVVRGYSGYNTRWALKVLERVFPEY 60
           MRP+IYLFGDSITEFSFADGGWGAALA+HFSRR DVVVRGYSGYNTRWALKVLERVFP  
Sbjct: 1   MRPRIYLFGDSITEFSFADGGWGAALANHFSRRADVVVRGYSGYNTRWALKVLERVFPAS 60

Query: 61  CDGGGDGAPLAVTVFFGANDACLPDGYGAFQHVPLHEYKQNLNSIVSFFKKKWPATRILL 120
            +  G G PLA+TVFFGANDACLPD YGAFQHVPL EYKQNL SIVSF KK+WPATRILL
Sbjct: 61  EEHSGAGDPLALTVFFGANDACLPDRYGAFQHVPLDEYKQNLISIVSFLKKQWPATRILL 120

Query: 121 ITPPPIDEEGRLLNPYIKNPSNEPERTNNAAGDYAKACIAVATECGVPVIDIWTKMQQIP 180
           ITPPPIDEEGRL NPY++NP NEPERTNNAAGDYAKACIAVA ECGV VIDIWTKMQQ+P
Sbjct: 121 ITPPPIDEEGRLQNPYLENPLNEPERTNNAAGDYAKACIAVAAECGVSVIDIWTKMQQLP 180

Query: 181 GWEKACLSDGLHLTENGNRVVFKEVIQKLREEGLSSETLPADLPLFSEIDFNDPLKAFEK 240
           GWEKACLSDGLHL+ NGN +VF+EV+QKL+EEGLS E LPADLPL SE+D+NDPLKAFE+
Sbjct: 181 GWEKACLSDGLHLSRNGNTIVFEEVVQKLKEEGLSPEMLPADLPLLSEMDYNDPLKAFEE 240

[Close]

BLAST of MC01g1487 vs. ExPASy TrEMBL
Match: A0A0A0KI83 (SGNH_hydro domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G518920 PE=4 SV=1)

HSP 1 Score: 421 bits (1083), Expect = 5.71e-148
Identity = 200/241 (82.99%), Postives = 217/241 (90.04%), Query Frame = 0


Query: 1   MRPKIYLFGDSITEFSFADGGWGAALAHHFSRRVDVVVRGYSGYNTRWALKVLERVFPEY 60
           MRPKIYLFGDSITEFSFADGGWG+AL +HF+R+ DVVVRGYSGYNTRWALKV+ERVFP  
Sbjct: 1   MRPKIYLFGDSITEFSFADGGWGSALTNHFTRKADVVVRGYSGYNTRWALKVIERVFPP- 60

Query: 61  CDGGGDGAPLAVTVFFGANDACLPDGYGAFQHVPLHEYKQNLNSIVSFFKKKWPATRILL 120
            +     +PLAVTVFFGANDACLPD YGAFQHVPLHEYKQNL SIVSFFKKKWPATRILL
Sbjct: 61  SEEREAASPLAVTVFFGANDACLPDRYGAFQHVPLHEYKQNLISIVSFFKKKWPATRILL 120

Query: 121 ITPPPIDEEGRLLNPYIKNPSNEPERTNNAAGDYAKACIAVATECGVPVIDIWTKMQQIP 180
           ITPPPIDEEGRL NPY+ NP NEPERTN+AAG YAKACIAVA ECGV VIDIWTKMQQ+P
Sbjct: 121 ITPPPIDEEGRLQNPYVSNPVNEPERTNDAAGAYAKACIAVAKECGVSVIDIWTKMQQVP 180

Query: 181 GWEKACLSDGLHLTENGNRVVFKEVIQKLREEGLSSETLPADLPLFSEIDFNDPLKAFEK 240
           GWEKACLSDGLHLT NGN +VF+EV+++L EEGLS  TLPADLPL SEIDFN+PL+AFEK
Sbjct: 181 GWEKACLSDGLHLTRNGNTIVFEEVVKRLEEEGLSPVTLPADLPLISEIDFNNPLQAFEK 240

[Close]

BLAST of MC01g1487 vs. ExPASy TrEMBL
Match: A0A5D3CMZ1 (GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G008030 PE=4 SV=1)

HSP 1 Score: 418 bits (1074), Expect = 1.34e-146
Identity = 198/241 (82.16%), Postives = 216/241 (89.63%), Query Frame = 0


Query: 1   MRPKIYLFGDSITEFSFADGGWGAALAHHFSRRVDVVVRGYSGYNTRWALKVLERVFPEY 60
           MRPKIYLFGDSITEFSFADGGWG+AL +HF+R+ DVVVRGYSGYNTRWALKV+ERVFP  
Sbjct: 1   MRPKIYLFGDSITEFSFADGGWGSALTNHFTRKADVVVRGYSGYNTRWALKVIERVFPP- 60

Query: 61  CDGGGDGAPLAVTVFFGANDACLPDGYGAFQHVPLHEYKQNLNSIVSFFKKKWPATRILL 120
            +     +PLAVTVFFGANDACLPD YGAFQHVPLHEYKQNL SIV+FFKKKWPATRILL
Sbjct: 61  SEEREAASPLAVTVFFGANDACLPDRYGAFQHVPLHEYKQNLISIVTFFKKKWPATRILL 120

Query: 121 ITPPPIDEEGRLLNPYIKNPSNEPERTNNAAGDYAKACIAVATECGVPVIDIWTKMQQIP 180
           ITPPPIDEEGRL NPY+ NP NEPERTN+AAG YA ACIAVA ECGV VIDIWTKMQQ+P
Sbjct: 121 ITPPPIDEEGRLQNPYVPNPLNEPERTNDAAGAYANACIAVAKECGVSVIDIWTKMQQVP 180

Query: 181 GWEKACLSDGLHLTENGNRVVFKEVIQKLREEGLSSETLPADLPLFSEIDFNDPLKAFEK 240
           GWEKACLSDGLHLT NGN +VF+EV+++L EEGLS ETLPADLPLFSE+D  +PLKAFEK
Sbjct: 181 GWEKACLSDGLHLTRNGNTIVFEEVVKRLEEEGLSPETLPADLPLFSEMDSTNPLKAFEK 240

[Close]

BLAST of MC01g1487 vs. TAIR 10
Match: AT5G45920.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 342.4 bits (877), Expect = 2.9e-94
Identity = 160/240 (66.67%), Postives = 190/240 (79.17%), Query Frame = 0


Query: 1   MRPKIYLFGDSITEFSFADGGWGAALAHHFSRRVDVVVRGYSGYNTRWALKVLERVFPEY 60
           MR KI+LFGDSITE SF+DGGWGA+LA    R+ D+V+RGYSGYNTRWALKV+ERVFP  
Sbjct: 2   MRRKIFLFGDSITEESFSDGGWGASLADLLRRKADMVLRGYSGYNTRWALKVVERVFPVA 61

Query: 61  CDGGGDGAPLAVTVFFGANDACLPDGYGAFQHVPLHEYKQNLNSIVSFFKKKWPATRILL 120
            + GGD +P AVTVFFGANDACLP+    FQHVPLHEYKQNL SIVSF K +WP T I+L
Sbjct: 62  EEDGGD-SPAAVTVFFGANDACLPERCSGFQHVPLHEYKQNLRSIVSFLKNRWPQTAIIL 121

Query: 121 ITPPPIDEEGRLLNPYIKNPSNEPERTNNAAGDYAKACIAVATECGVPVIDIWTKMQQIP 180
           ITPPPIDEE RL  PYI+N +  PERTN  AG YAKACIAVA EC + V D+W+KMQQIP
Sbjct: 122 ITPPPIDEEARLRYPYIENTTGLPERTNEVAGLYAKACIAVAEECQISVTDLWSKMQQIP 181

Query: 181 GWEKACLSDGLHLTENGNRVVFKEVIQKLREEGLSSETLPADLPLFSEIDFNDPLKAFEK 240
            W+  CL DGLHL+  GN+VVF+EV +KL+EEG+ +E L  DLPL  ++D  DPLK+F++
Sbjct: 182 NWQTECLWDGLHLSRVGNKVVFEEVAKKLKEEGIGAEDLAVDLPLIEDVDPKDPLKSFDE 240

[Close]

BLAST of MC01g1487 vs. TAIR 10
Match: AT5G62930.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 251.1 bits (640), Expect = 8.8e-67
Identity = 128/243 (52.67%), Postives = 155/243 (63.79%), Query Frame = 0


Query: 1   MRPKIYLFGDSITEFSFADGGWGAALAHHFSRRVDVVVRGYSGYNTRWALKVLERVFPEY 60
           MRP+I LFGDSIT  SF  GGWG+ALA  +SR+ DVVVRGY GYNTRWAL +L  +FP  
Sbjct: 1   MRPEIVLFGDSITAQSFRSGGWGSALADAYSRKADVVVRGYGGYNTRWALFLLHHIFPL- 60

Query: 61  CDGGGDGAPLAVTVFFGANDACLPDGYGAFQHVPLHEYKQNLNSIVSFFKKKWPATRILL 120
              G    P+A T+FFGANDA L       QHVP+ EY  N+  IV   KK  P   I+L
Sbjct: 61  ---GSSSPPVATTIFFGANDAALKGRTSDRQHVPVEEYTDNVRKIVQHLKKCSPTMLIVL 120

Query: 121 ITPPPIDEEGR---LLNPYIKNPSNEPERTNNAAGDYAKACIAVATECGVPVIDIWTKMQ 180
           ITPPPIDE GR     + Y +    EPERTN   G YA+ C+A+A E G+  +++W+KMQ
Sbjct: 121 ITPPPIDEAGRQSYAESIYGEKAMKEPERTNETTGVYAQHCVALAEELGLRCVNLWSKMQ 180

Query: 181 QIPGWEKACLSDGLHLTENGNRVVFKEVIQKLREEGLSSETLPADLPLFSEIDFNDPLKA 240
           +   W+K  LSDGLHLT  GN VVF EV +  RE  LS E +P D P  S ID  +P KA
Sbjct: 181 ETNDWQKKYLSDGLHLTPEGNGVVFDEVSRVFREAWLSPEEMPFDFPHHSHIDGKNPSKA 239

[Close]

BLAST of MC01g1487 vs. TAIR 10
Match: AT2G38180.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 167.9 bits (424), Expect = 9.8e-42
Identity = 90/236 (38.14%), Postives = 130/236 (55.08%), Query Frame = 0


Query: 1   MRPKIYLFGDSITEFSFADGGWGAALAHHFSRRVDVVVRGYSGYNTRWALKVLERVFPEY 60
           +RP+I LFG SI ++SF D GWGA LA  +SR  D+++RGY+G+N+R+ALKVL +VFP+ 
Sbjct: 5   VRPQIVLFGSSIVQYSFTDRGWGATLADLYSRTADIILRGYAGWNSRFALKVLHQVFPK- 64

Query: 61  CDGGGDGAPLAVTVFFGANDACLPDGYGAFQHVPLHEYKQNLNSIVSFFKKKWPATRILL 120
                   P  V V+FG ND+  P   G   HVPL E+ +N+  I          TR++ 
Sbjct: 65  ---DAVIQPSLVIVYFGGNDSTHPHPSGHGPHVPLSEFIENMRKIGEHLLSLSDKTRVIF 124

Query: 121 ITPPPIDEE--GRLLNPYIKNPSNEPERTNNAAGDYAKACIAVATECGVPVIDIWTKMQQ 180
           +TPPP++E+    +    IK  SNE  R       YA+  + +  E  V  IDIWT +QQ
Sbjct: 125 LTPPPMNEKQIEIVFGDAIKGRSNELCR------PYAEELLNLCREINVKGIDIWTAIQQ 184

Query: 181 IPGWEKACLSDGLHLTENGNRVVFKEVIQKLREEGLSSETLPADLPLFSEIDFNDP 235
              W  +C +DG+H T   + +V KE+++ LR            LP+    DF+ P
Sbjct: 185 QDDWLNSCFTDGIHFTAKASEIVVKEILKVLRGADWKPSLYWKSLPVEFPFDFDAP 230

[Close]

BLAST of MC01g1487 vs. TAIR 10
Match: AT3G11210.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 165.2 bits (417), Expect = 6.3e-41
Identity = 80/214 (37.38%), Postives = 128/214 (59.81%), Query Frame = 0


Query: 2   RPKIYLFGDSITEFSFADGGWGAALAHHFSRRVDVVVRGYSGYNTRWALKVLERVFPEYC 61
           RP+I LFG SI + SF  GGWGA L+  ++R+ D+++RGY G+N+  AL+V+++VFP+  
Sbjct: 6   RPQIVLFGSSIVQMSFGHGGWGAILSEVYARKADIILRGYYGWNSSRALEVVDQVFPK-- 65

Query: 62  DGGGDGA--PLAVTVFFGANDACLPDGYGAFQHVPLHEYKQNLNSIVSFFKKKWPATRIL 121
               D A  P  V V+FG ND+  P   G   HVPL EY  N+  I    +     TRI+
Sbjct: 66  ----DAAVQPSLVIVYFGGNDSMAPHSSGLGPHVPLTEYVDNMKKIALHLQSLSDFTRII 125

Query: 122 LITPPPIDEEGRLLN--PYIKNPSNEPERTNNAAGDYAKACIAVATECGVPVIDIWTKMQ 181
            ++ PP+DE     N  PY+    +E  RTN+    Y+ AC+ +  E G+ V+D+++  Q
Sbjct: 126 FLSSPPVDEAKVRQNQSPYL----SEVIRTNDLCKTYSDACVELCQELGLEVVDLFSTFQ 185

Query: 182 QIPGWEKACLSDGLHLTENGNRVVFKEVIQKLRE 212
           +   W+  C +DG+HL+  G+++V  E+++ ++E
Sbjct: 186 KADDWKTVCFTDGIHLSAQGSKIVAGEILRVVKE 209

The following BLAST results are available for this feature:

BLAST of MC01g1487 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6NMR9	4.1e-93	66.67	GDSL esterase/lipase At5g45920 OS=Arabidopsis thaliana OX=3702 GN=At5g45920 PE=2...	[more]
Q9FM04	1.2e-65	52.67	GDSL esterase/lipase At5g62930 OS=Arabidopsis thaliana OX=3702 GN=At5g62930 PE=2...	[more]
Q711G3	4.1e-45	44.21	Isoamyl acetate-hydrolyzing esterase 1 homolog OS=Rattus norvegicus OX=10116 GN=...	[more]
Q9DB29	1.6e-44	42.49	Isoamyl acetate-hydrolyzing esterase 1 homolog OS=Mus musculus OX=10090 GN=Iah1 ...	[more]
Q3SZ16	2.1e-44	44.30	Isoamyl acetate-hydrolyzing esterase 1 homolog OS=Bos taurus OX=9913 GN=IAH1 PE=...	[more]

BLAST of MC01g1487 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022142319.1	6.11e-182	100.00	GDSL esterase/lipase At5g45920 isoform X1 [Momordica charantia] >XP_022142320.1 ...	[more]
XP_022978753.1	4.83e-152	85.06	GDSL esterase/lipase At5g45920 [Cucurbita maxima]	[more]
XP_022925816.1	6.86e-152	84.65	GDSL esterase/lipase At5g45920 [Cucurbita moschata] >KAG7034633.1 GDSL esterase/...	[more]
XP_038882981.1	7.64e-152	84.49	GDSL esterase/lipase At5g45920 [Benincasa hispida] >XP_038882982.1 GDSL esterase...	[more]
XP_023544567.1	8.00e-151	84.23	GDSL esterase/lipase At5g45920 [Cucurbita pepo subsp. pepo]	[more]

BLAST of MC01g1487 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CLU7	2.96e-182	100.00	GDSL esterase/lipase At5g45920 isoform X1 OS=Momordica charantia OX=3673 GN=LOC1...	[more]
A0A6J1IU69	2.34e-152	85.06	GDSL esterase/lipase At5g45920 OS=Cucurbita maxima OX=3661 GN=LOC111478618 PE=3 ...	[more]
A0A6J1ED78	3.32e-152	84.65	GDSL esterase/lipase At5g45920 OS=Cucurbita moschata OX=3662 GN=LOC111433110 PE=...	[more]
A0A0A0KI83	5.71e-148	82.99	SGNH_hydro domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G518920 ...	[more]
A0A5D3CMZ1	1.34e-146	82.16	GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255...	[more]

BLAST of MC01g1487 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G45920.1	2.9e-94	66.67	SGNH hydrolase-type esterase superfamily protein	[more]
AT5G62930.1	8.8e-67	52.67	SGNH hydrolase-type esterase superfamily protein	[more]
AT2G38180.1	9.8e-42	38.14	SGNH hydrolase-type esterase superfamily protein	[more]
AT3G11210.1	6.3e-41	37.38	SGNH hydrolase-type esterase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036514	SGNH hydrolase superfamily	GENE3D	3.40.50.1110	SGNH hydrolase	coord: 2..237 e-value: 1.3E-74 score: 252.5
IPR013830	SGNH hydrolase-type esterase domain	PFAM	PF13472	Lipase_GDSL_2	coord: 7..199 e-value: 1.5E-28 score: 100.5
None	No IPR available	PANTHER	PTHR14209:SF26	ISOAMYL ACETATE-HYDROLYZING ESTERASE 1 HOMOLOG	coord: 1..239
None	No IPR available	CDD	cd01838	Isoamyl_acetate_hydrolase_like	coord: 4..209 e-value: 6.22037E-99 score: 284.915
None	No IPR available	SUPERFAMILY	52266	SGNH hydrolase	coord: 3..211
IPR045136	Isoamyl acetate-hydrolyzing esterase Iah1-like	PANTHER	PTHR14209	ISOAMYL ACETATE-HYDROLYZING ESTERASE 1	coord: 1..239

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC01g1487.1	MC01g1487.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016788	hydrolase activity, acting on ester bonds