MC01g1462 (gene) Bitter gourd (Dali-11) v1

BLAST of MC01g1462 vs. ExPASy Swiss-Prot
Match: P16128 (Uncharacterized protein At4g18490 OS=Arabidopsis thaliana OX=3702 GN=At4g18490 PE=4 SV=3)

HSP 1 Score: 168.3 bits (425), Expect = 3.3e-40
Identity = 217/777 (27.93%), Postives = 352/777 (45.30%), Query Frame = 0

Query: 10  SATDLRKKDTLLDEDIGDEFMNSWKSISVAEDD-MDFSFGTVSKGKNKAFDFGTLDVDFN 69
           S+TD + KD +L  D  D   ++W   S+ +DD MDF FG+ +K K  AF    LD+ F+
Sbjct: 8   SSTDTQDKDLMLAAD-KDMEKDTWNFKSMTDDDPMDFGFGSPAKNKKNAF---KLDMGFD 67

Query: 70  LDGSFEKLSSFKMDMSDLEFSCSPKKTEKARSSGKEESPNENLQKDIDSLNFSFDFKELD 129
           LDG F   SSFKMDM D +FS   KKT K + +  ++ P+ N ++  +   FS+DF  LD
Sbjct: 68  LDGDFG--SSFKMDMPDFDFSSPAKKTTKTKET-SDDKPSGNSKQKKNPFAFSYDFDALD 127

Query: 130 SFDVGKS-LQNGERSCNRKQDTRAVCSSR-----------VDLAASNIHIAEENTAIDNS 189
            FD+G S  + G ++  +  D   +C+S            +DL  +    ++ NT +   
Sbjct: 128 DFDLGSSPPKKGSKTTTKSMDCEEICASSKSDKSDDLDFGLDLPITRQVPSKANTDVQAK 187

Query: 190 ITKILPASGKETSSKV---QNFQGGRGELES----EVVDGTSHEAINAVQT--------- 249
            +    +   +T+  +   ++    +  LES    E V+        A QT         
Sbjct: 188 ASAEKESQNYKTTDTLVVNKSKNSNQAALESMGDFEAVESPQGSRKKASQTHTMCVQPQS 247

Query: 250 ---------TNKEEQLEKGCLSEKEVSKSSNLAIHDVPIKCIARNYAPECTSEPQPDIR- 309
                     +K E+  + C S + ++ S    +H   I  IA N       E  PDI  
Sbjct: 248 VDTSPLKTSCSKVEEKNEPCPSNETIAPS---PLHASEIAHIAVN------RETSPDIHE 307

Query: 310 -TTSGELRVVSGVTENVTDEIIDSDVTCCKNLPQKDLSPII---LCAPESNHTEEDKSEC 369
              SG         EN   ++I +  +  + + Q   SP I   LC+ +  H +E+    
Sbjct: 308 LCRSGTKEDCPIDPENANKKMITTMESSYEKIEQ--TSPSISSHLCSDKIEHQQEEMG-T 367

Query: 370 STPNEVVDKIQLAEVRLDLRDRSNSDVQRKLLPDPQ--EIRENRNLNLKLPTIPLCRGTP 429
            T  E+ D  + A    D    S + +  K+ P  +  +  + ++L+ KLP  P      
Sbjct: 368 DTQAEIQDNTKGALYNSD-AGHSLTTLSGKISPGTRTSQTAKVQDLSEKLPLDPSHSMAG 427

Query: 430 VRKVTVKEREIDGNSSTSRTDVVSKPQLH--QSSPISTKLFTLGKNRIDVSNQTPATGDG 489
           +  +   + +  G   +       KP+ H  +SSPI T++  + +  I  S+  P     
Sbjct: 428 LDNLRAMQNKDLGLIRSRFFKKPEKPESHVLESSPIETEIQPVIRENIG-SSLNPT---- 487

Query: 490 KLCRDSQPHNKVANTIPPVVVQSEKSYGKLFGLSYSYSARVTPSNSCVKTTTQTHCNSEL 549
               D+  H K+ +         E   G++  L    +   T   S ++    +  ++  
Sbjct: 488 ---NDTSSHEKIIHKDHSNGKTVENVAGQMDHLKLQ-AKNTTREKSILQINISSKLDASS 547

Query: 550 LKLSTVPSQSLKIISAQESKLRSI---KSSLIFPNLSSLKTSWAFGGKQILSSTGREKER 609
           L        S  + S Q+ KL S+   K   I  +L ++KT    GG +   S+  + E 
Sbjct: 548 LTQKLSNHLSSGVESLQKPKLNSLGRPKLGNIMADLRAVKTQRTIGGNKDQPSSAVQPEF 607

Query: 610 KSGDSEQTTEAGQRSKRLDIGYCTENAEKQNL-LISNMKRKALEESNADSMLLKPLKRLS 669
            S  S++        K  +I +     + Q L   S++KRKAL+E +AD  L   LKRLS
Sbjct: 608 SSSISKERNTEAPVKKSSEIHHLAPRDKTQTLHCPSSLKRKALDE-DADRSLKPQLKRLS 667

Query: 670 VSPIGFRNSKEPLEKKIEEQVEGMTSNASHDHIASSI-ENPHVP------NTMELEISLD 729
           +SP   RN +E   + ++ +     S          + E+P         N   LEI + 
Sbjct: 668 MSPRENRNVEELTHRAVQGKFSSQESRIDTKTTKEPVKESPQTKSHYQNINMANLEIPI- 727

BLAST of MC01g1462 vs. NCBI nr
Match: XP_022142072.1 (uncharacterized protein At4g18490 isoform X1 [Momordica charantia] >XP_022142073.1 uncharacterized protein At4g18490 isoform X1 [Momordica charantia])

HSP 1 Score: 1330 bits (3443), Expect = 0.0
Identity = 709/748 (94.79%), Postives = 709/748 (94.79%), Query Frame = 0

Query: 1   MAESRKGASSATDLRKKDTLLDEDIGDEFMNSWKSISVAEDDMDFSFGTVSKGKNKAFDF 60
           MAESRKGASSATDLRKKDTLLDEDIGDEFMNSWKSISVAEDDMDFSFGTVSK KNKAFDF
Sbjct: 1   MAESRKGASSATDLRKKDTLLDEDIGDEFMNSWKSISVAEDDMDFSFGTVSKAKNKAFDF 60

Query: 61  GTLDVDFNLDGSFEKLSSFKMDMSDLEFSCSPKKTEKARSSGKEESPNENLQKDIDSLNF 120
           GTLDVDFNLDGSFEKLSSFKMDMSDLEFSCSPKKTEKARSSGKEESPNENLQKDIDSLNF
Sbjct: 61  GTLDVDFNLDGSFEKLSSFKMDMSDLEFSCSPKKTEKARSSGKEESPNENLQKDIDSLNF 120

Query: 121 SFDFKELDSFDVGKSLQNGERSCNRKQDTRAVCSSRVDLAASNIHIAEENTAIDNSITKI 180
           SFDFKELDSFDVGKSLQNGERSCNRKQDTRAVCSSRVDLAASNIHIAEENTAIDNSITKI
Sbjct: 121 SFDFKELDSFDVGKSLQNGERSCNRKQDTRAVCSSRVDLAASNIHIAEENTAIDNSITKI 180

Query: 181 LPASGKETSSKVQNFQGGRGELESEVVDGTSHEAINAVQTTNKEEQLEKGCLSEKEVSKS 240
           LPASGKETSSKVQNFQGGRGELESEVVDGTSHEAINAVQTTNKEEQLEKGCLSEKEVSKS
Sbjct: 181 LPASGKETSSKVQNFQGGRGELESEVVDGTSHEAINAVQTTNKEEQLEKGCLSEKEVSKS 240

Query: 241 SNLAIHDVPIKCIARNYAPECTSEPQPDIRTTSGELRVVSGVTENVTDEIIDSDVTCCKN 300
           SNLAIHDVPIKCIARNYAPECTSEPQPDIRTTSGELRVVSGVTENVTDEIIDSDVTCCKN
Sbjct: 241 SNLAIHDVPIKCIARNYAPECTSEPQPDIRTTSGELRVVSGVTENVTDEIIDSDVTCCKN 300

Query: 301 LPQKDLSPIILCAPESNHTEEDKSECSTPNEVVDKIQLAEVRLDLRDRSNSDVQRKLLPD 360
           LPQKDLSPIILCAPESNHTEEDKSECSTPNEVVDKIQLAEVRLDLRDRSNSDVQRKLLPD
Sbjct: 301 LPQKDLSPIILCAPESNHTEEDKSECSTPNEVVDKIQLAEVRLDLRDRSNSDVQRKLLPD 360

Query: 361 PQEIRENRNLNLKLPTIPLCRGTPVRKVTVKEREIDGNSSTSRTDVVSKPQLHQSSPIST 420
           PQEIRENRNLNLKLPTIPLCRGTPVRKVTVKEREIDGNSSTSRTDVVSKPQLHQSSPIST
Sbjct: 361 PQEIRENRNLNLKLPTIPLCRGTPVRKVTVKEREIDGNSSTSRTDVVSKPQLHQSSPIST 420

Query: 421 KLFTLGKNRIDVSNQTPATGDGKLCRDSQPHNKVANTIPPVVVQSEKSYGKLFGLSYSYS 480
           KLFTLGKNRIDVSNQTPATGDG                                      
Sbjct: 421 KLFTLGKNRIDVSNQTPATGDG-------------------------------------- 480

Query: 481 ARVTPSNSCVKTTTQTHCNSELLKLSTVPSQSLKIISAQESKLRSIKSSLIFPNLSSLKT 540
           ARVTPSNSCVKTTTQTHCNSELLKLSTVPSQSLKIISAQESKLRSIKSSLIFPNLSSLKT
Sbjct: 481 ARVTPSNSCVKTTTQTHCNSELLKLSTVPSQSLKIISAQESKLRSIKSSLIFPNLSSLKT 540

Query: 541 SWAFGGKQILSSTGREKERKSGDSEQTTEAGQRSKRLDIGYCTENAEKQNLLISNMKRKA 600
           SWAFGGKQILSSTGREKERKSGDSEQTTEAGQRSKRLDIGYCTENAEKQNLLISNMKRKA
Sbjct: 541 SWAFGGKQILSSTGREKERKSGDSEQTTEAGQRSKRLDIGYCTENAEKQNLLISNMKRKA 600

Query: 601 LEESNADSMLLKPLKRLSVSPIGFRNSKEPLEKKIEEQVEGMTSNASHDHIASSIENPHV 660
           LEESNADSMLLKPLKRLSVSPIGFRNSKEPLEKKIEEQVEGMTSNASHDHIASSIENPHV
Sbjct: 601 LEESNADSMLLKPLKRLSVSPIGFRNSKEPLEKKIEEQVEGMTSNASHDHIASSIENPHV 660

Query: 661 PNTMELEISLDLENDRNVEKADAYSRELEDICNMLRKKHDEAKEMLVRVIVNNSNLLMLN 720
           PNTMELEISLDLENDRNVEKADAYSRELEDICNMLRKKHDEAKEMLVRVIVNNSNLLMLN
Sbjct: 661 PNTMELEISLDLENDRNVEKADAYSRELEDICNMLRKKHDEAKEMLVRVIVNNSNLLMLN 710

Query: 721 HPIFEDKIRKVQKFAAKLLSKELQTKAA 748
           HPIFEDKIRKVQKFAAKLLSKELQTKAA
Sbjct: 721 HPIFEDKIRKVQKFAAKLLSKELQTKAA 710

BLAST of MC01g1462 vs. NCBI nr
Match: XP_022142074.1 (uncharacterized protein At4g18490 isoform X2 [Momordica charantia])

HSP 1 Score: 1214 bits (3140), Expect = 0.0
Identity = 648/687 (94.32%), Postives = 648/687 (94.32%), Query Frame = 0

Query: 62  TLDVDFNLDGSFEKLSSFKMDMSDLEFSCSPKKTEKARSSGKEESPNENLQKDIDSLNFS 121
           T DVDFNLDGSFEKLSSFKMDMSDLEFSCSPKKTEKARSSGKEESPNENLQKDIDSLNFS
Sbjct: 2   TWDVDFNLDGSFEKLSSFKMDMSDLEFSCSPKKTEKARSSGKEESPNENLQKDIDSLNFS 61

Query: 122 FDFKELDSFDVGKSLQNGERSCNRKQDTRAVCSSRVDLAASNIHIAEENTAIDNSITKIL 181
           FDFKELDSFDVGKSLQNGERSCNRKQDTRAVCSSRVDLAASNIHIAEENTAIDNSITKIL
Sbjct: 62  FDFKELDSFDVGKSLQNGERSCNRKQDTRAVCSSRVDLAASNIHIAEENTAIDNSITKIL 121

Query: 182 PASGKETSSKVQNFQGGRGELESEVVDGTSHEAINAVQTTNKEEQLEKGCLSEKEVSKSS 241
           PASGKETSSKVQNFQGGRGELESEVVDGTSHEAINAVQTTNKEEQLEKGCLSEKEVSKSS
Sbjct: 122 PASGKETSSKVQNFQGGRGELESEVVDGTSHEAINAVQTTNKEEQLEKGCLSEKEVSKSS 181

Query: 242 NLAIHDVPIKCIARNYAPECTSEPQPDIRTTSGELRVVSGVTENVTDEIIDSDVTCCKNL 301
           NLAIHDVPIKCIARNYAPECTSEPQPDIRTTSGELRVVSGVTENVTDEIIDSDVTCCKNL
Sbjct: 182 NLAIHDVPIKCIARNYAPECTSEPQPDIRTTSGELRVVSGVTENVTDEIIDSDVTCCKNL 241

Query: 302 PQKDLSPIILCAPESNHTEEDKSECSTPNEVVDKIQLAEVRLDLRDRSNSDVQRKLLPDP 361
           PQKDLSPIILCAPESNHTEEDKSECSTPNEVVDKIQLAEVRLDLRDRSNSDVQRKLLPDP
Sbjct: 242 PQKDLSPIILCAPESNHTEEDKSECSTPNEVVDKIQLAEVRLDLRDRSNSDVQRKLLPDP 301

Query: 362 QEIRENRNLNLKLPTIPLCRGTPVRKVTVKEREIDGNSSTSRTDVVSKPQLHQSSPISTK 421
           QEIRENRNLNLKLPTIPLCRGTPVRKVTVKEREIDGNSSTSRTDVVSKPQLHQSSPISTK
Sbjct: 302 QEIRENRNLNLKLPTIPLCRGTPVRKVTVKEREIDGNSSTSRTDVVSKPQLHQSSPISTK 361

Query: 422 LFTLGKNRIDVSNQTPATGDGKLCRDSQPHNKVANTIPPVVVQSEKSYGKLFGLSYSYSA 481
           LFTLGKNRIDVSNQTPATGDG                                      A
Sbjct: 362 LFTLGKNRIDVSNQTPATGDG--------------------------------------A 421

Query: 482 RVTPSNSCVKTTTQTHCNSELLKLSTVPSQSLKIISAQESKLRSIKSSLIFPNLSSLKTS 541
           RVTPSNSCVKTTTQTHCNSELLKLSTVPSQSLKIISAQESKLRSIKSSLIFPNLSSLKTS
Sbjct: 422 RVTPSNSCVKTTTQTHCNSELLKLSTVPSQSLKIISAQESKLRSIKSSLIFPNLSSLKTS 481

Query: 542 WAFGGKQILSSTGREKERKSGDSEQTTEAGQRSKRLDIGYCTENAEKQNLLISNMKRKAL 601
           WAFGGKQILSSTGREKERKSGDSEQTTEAGQRSKRLDIGYCTENAEKQNLLISNMKRKAL
Sbjct: 482 WAFGGKQILSSTGREKERKSGDSEQTTEAGQRSKRLDIGYCTENAEKQNLLISNMKRKAL 541

Query: 602 EESNADSMLLKPLKRLSVSPIGFRNSKEPLEKKIEEQVEGMTSNASHDHIASSIENPHVP 661
           EESNADSMLLKPLKRLSVSPIGFRNSKEPLEKKIEEQVEGMTSNASHDHIASSIENPHVP
Sbjct: 542 EESNADSMLLKPLKRLSVSPIGFRNSKEPLEKKIEEQVEGMTSNASHDHIASSIENPHVP 601

Query: 662 NTMELEISLDLENDRNVEKADAYSRELEDICNMLRKKHDEAKEMLVRVIVNNSNLLMLNH 721
           NTMELEISLDLENDRNVEKADAYSRELEDICNMLRKKHDEAKEMLVRVIVNNSNLLMLNH
Sbjct: 602 NTMELEISLDLENDRNVEKADAYSRELEDICNMLRKKHDEAKEMLVRVIVNNSNLLMLNH 650

Query: 722 PIFEDKIRKVQKFAAKLLSKELQTKAA 748
           PIFEDKIRKVQKFAAKLLSKELQTKAA
Sbjct: 662 PIFEDKIRKVQKFAAKLLSKELQTKAA 650

BLAST of MC01g1462 vs. NCBI nr
Match: XP_022142075.1 (uncharacterized protein At4g18490 isoform X3 [Momordica charantia])

HSP 1 Score: 1180 bits (3052), Expect = 0.0
Identity = 630/668 (94.31%), Postives = 630/668 (94.31%), Query Frame = 0

Query: 81  MDMSDLEFSCSPKKTEKARSSGKEESPNENLQKDIDSLNFSFDFKELDSFDVGKSLQNGE 140
           MDMSDLEFSCSPKKTEKARSSGKEESPNENLQKDIDSLNFSFDFKELDSFDVGKSLQNGE
Sbjct: 1   MDMSDLEFSCSPKKTEKARSSGKEESPNENLQKDIDSLNFSFDFKELDSFDVGKSLQNGE 60

Query: 141 RSCNRKQDTRAVCSSRVDLAASNIHIAEENTAIDNSITKILPASGKETSSKVQNFQGGRG 200
           RSCNRKQDTRAVCSSRVDLAASNIHIAEENTAIDNSITKILPASGKETSSKVQNFQGGRG
Sbjct: 61  RSCNRKQDTRAVCSSRVDLAASNIHIAEENTAIDNSITKILPASGKETSSKVQNFQGGRG 120

Query: 201 ELESEVVDGTSHEAINAVQTTNKEEQLEKGCLSEKEVSKSSNLAIHDVPIKCIARNYAPE 260
           ELESEVVDGTSHEAINAVQTTNKEEQLEKGCLSEKEVSKSSNLAIHDVPIKCIARNYAPE
Sbjct: 121 ELESEVVDGTSHEAINAVQTTNKEEQLEKGCLSEKEVSKSSNLAIHDVPIKCIARNYAPE 180

Query: 261 CTSEPQPDIRTTSGELRVVSGVTENVTDEIIDSDVTCCKNLPQKDLSPIILCAPESNHTE 320
           CTSEPQPDIRTTSGELRVVSGVTENVTDEIIDSDVTCCKNLPQKDLSPIILCAPESNHTE
Sbjct: 181 CTSEPQPDIRTTSGELRVVSGVTENVTDEIIDSDVTCCKNLPQKDLSPIILCAPESNHTE 240

Query: 321 EDKSECSTPNEVVDKIQLAEVRLDLRDRSNSDVQRKLLPDPQEIRENRNLNLKLPTIPLC 380
           EDKSECSTPNEVVDKIQLAEVRLDLRDRSNSDVQRKLLPDPQEIRENRNLNLKLPTIPLC
Sbjct: 241 EDKSECSTPNEVVDKIQLAEVRLDLRDRSNSDVQRKLLPDPQEIRENRNLNLKLPTIPLC 300

Query: 381 RGTPVRKVTVKEREIDGNSSTSRTDVVSKPQLHQSSPISTKLFTLGKNRIDVSNQTPATG 440
           RGTPVRKVTVKEREIDGNSSTSRTDVVSKPQLHQSSPISTKLFTLGKNRIDVSNQTPATG
Sbjct: 301 RGTPVRKVTVKEREIDGNSSTSRTDVVSKPQLHQSSPISTKLFTLGKNRIDVSNQTPATG 360

Query: 441 DGKLCRDSQPHNKVANTIPPVVVQSEKSYGKLFGLSYSYSARVTPSNSCVKTTTQTHCNS 500
           DG                                      ARVTPSNSCVKTTTQTHCNS
Sbjct: 361 DG--------------------------------------ARVTPSNSCVKTTTQTHCNS 420

Query: 501 ELLKLSTVPSQSLKIISAQESKLRSIKSSLIFPNLSSLKTSWAFGGKQILSSTGREKERK 560
           ELLKLSTVPSQSLKIISAQESKLRSIKSSLIFPNLSSLKTSWAFGGKQILSSTGREKERK
Sbjct: 421 ELLKLSTVPSQSLKIISAQESKLRSIKSSLIFPNLSSLKTSWAFGGKQILSSTGREKERK 480

Query: 561 SGDSEQTTEAGQRSKRLDIGYCTENAEKQNLLISNMKRKALEESNADSMLLKPLKRLSVS 620
           SGDSEQTTEAGQRSKRLDIGYCTENAEKQNLLISNMKRKALEESNADSMLLKPLKRLSVS
Sbjct: 481 SGDSEQTTEAGQRSKRLDIGYCTENAEKQNLLISNMKRKALEESNADSMLLKPLKRLSVS 540

Query: 621 PIGFRNSKEPLEKKIEEQVEGMTSNASHDHIASSIENPHVPNTMELEISLDLENDRNVEK 680
           PIGFRNSKEPLEKKIEEQVEGMTSNASHDHIASSIENPHVPNTMELEISLDLENDRNVEK
Sbjct: 541 PIGFRNSKEPLEKKIEEQVEGMTSNASHDHIASSIENPHVPNTMELEISLDLENDRNVEK 600

Query: 681 ADAYSRELEDICNMLRKKHDEAKEMLVRVIVNNSNLLMLNHPIFEDKIRKVQKFAAKLLS 740
           ADAYSRELEDICNMLRKKHDEAKEMLVRVIVNNSNLLMLNHPIFEDKIRKVQKFAAKLLS
Sbjct: 601 ADAYSRELEDICNMLRKKHDEAKEMLVRVIVNNSNLLMLNHPIFEDKIRKVQKFAAKLLS 630

Query: 741 KELQTKAA 748
           KELQTKAA
Sbjct: 661 KELQTKAA 630

BLAST of MC01g1462 vs. NCBI nr
Match: XP_022925825.1 (uncharacterized protein At4g18490 [Cucurbita moschata] >XP_022925826.1 uncharacterized protein At4g18490 [Cucurbita moschata])

HSP 1 Score: 998 bits (2580), Expect = 0.0
Identity = 554/750 (73.87%), Postives = 609/750 (81.20%), Query Frame = 0

Query: 1   MAESRKGASSATDLRKKDTLLDEDIGDEFMNSWKSISVAEDDM-DFSFGTVSKGKNKAFD 60
           MAE RKGAS ATD RKKD+LLDEDIGDEFMNSWKSISVAEDDM DFSFGTVSKGKNKAFD
Sbjct: 1   MAEPRKGASLATDPRKKDSLLDEDIGDEFMNSWKSISVAEDDMVDFSFGTVSKGKNKAFD 60

Query: 61  FGTLDVDFNLDGSFEKLSSFKMDMSDLEFSCSPKKTEKARSSGKEESPNENLQKDIDSLN 120
           FGTLDVDFNLDGSFEKLSSFK+DM DL+FS  PKK EKARSSGKE SP ENLQ+D+DSLN
Sbjct: 61  FGTLDVDFNLDGSFEKLSSFKIDMPDLDFSSPPKKNEKARSSGKEGSPKENLQRDMDSLN 120

Query: 121 FSFDFKELDSFDVGKSLQNGERSCNRKQDTRAVCSSRVDLAASNIHIAEENTAIDN-SIT 180
           FSFDFKELDSFDV KSLQNGER+C ++QDT AV SSRV+  A NIHI EENTA DN SI 
Sbjct: 121 FSFDFKELDSFDVDKSLQNGERTCKQQQDTEAVSSSRVEHEAFNIHIGEENTATDNTSIA 180

Query: 181 KILPASGKETSSKVQNFQGGRGELESEVVDGTSHEAINAVQTTNKEEQLEKGCLSEKEVS 240
           K LPASG ETSS+V+NF G  GELESEV DGTSHEA N    TN     EKGCL EKE+S
Sbjct: 181 KRLPASGNETSSRVENFHGDHGELESEVADGTSHEARNTAPATN-----EKGCLPEKELS 240

Query: 241 KSSNLAIHDVPIKCIARNYAPECTSEPQPDIRTTSGELRVVSGVTENVTDEIIDSDVTCC 300
           K S+  IHDVP++CIARNYAPECTSEPQ +I    GEL VVSG T  VTDEIIDSD  CC
Sbjct: 241 KGSHQVIHDVPVRCIARNYAPECTSEPQSEICAEKGELTVVSGGTGKVTDEIIDSDGACC 300

Query: 301 KNLPQKDLSPIILCAPESNHTEEDKSECSTPNEVVDKIQLAEVRLDLRDRSNSDVQRKLL 360
           K LP   LS I L A E N TE++KSE S  NEVVD +QLAEV L L+D SNSD  RKLL
Sbjct: 301 KKLPHSYLSSINLSASERNPTEKNKSEYSHLNEVVDNVQLAEVHLVLKDCSNSDAPRKLL 360

Query: 361 PDPQEIRENRNLNLKLPTIPLCRGTPVRKVTVKEREIDGNSSTSRTDVVSKPQLHQSSPI 420
            D QEIREN N  LK   +PLCRG PV +VTVKE+E+ GN S S+TDVV K QL+Q+S I
Sbjct: 361 LDTQEIRENHNSKLKPSIVPLCRGLPVNEVTVKEKEVSGNYSISKTDVVRKSQLYQASFI 420

Query: 421 STKLFTLGKNRIDVSNQTPATGDGKLCRDSQPHNKVANTIPPVVVQSEKSYGKLFGLSYS 480
           STKLFTLGKNRID  NQ PA GD   CRDS+P NK+ANT PPVVVQSEKS GKL     +
Sbjct: 421 STKLFTLGKNRIDAPNQIPAAGDRNRCRDSRPQNKLANTAPPVVVQSEKSVGKL----RA 480

Query: 481 YSARVTPSNSCVKTTTQTHCNSELLKLSTVPSQSLKIISAQESKLRSIKSSLIFPNLSSL 540
            S RV PSN C KTTTQ HCN EL KLS +P++S K ISAQESKL S+K+ LIFP LSSL
Sbjct: 481 SSPRVKPSNLCGKTTTQAHCNPELWKLSMIPNRSAKTISAQESKLCSLKTGLIFPGLSSL 540

Query: 541 KTSWAFGGKQILSSTGREKERKSGDSEQTTEAGQRSKRLDIGYCTENAEKQNLLISNMKR 600
           KT  AFGGKQ+ +STG  KERK G+SEQT EAGQRSK+LDIGYCTENAEKQ LLISNMKR
Sbjct: 541 KTYRAFGGKQVPTSTGTVKERKLGESEQT-EAGQRSKKLDIGYCTENAEKQELLISNMKR 600

Query: 601 KALEESNADSMLLKPLKRLSVSPIGFRNSKEPLEKKIEEQVEGMTSNASHDHIASSIENP 660
           KALEE NAD   LKPLK L VSP GFRN+KEPL++KIEEQVE MT+ ASHD +AS+IENP
Sbjct: 601 KALEEPNADPKGLKPLKHLCVSPSGFRNTKEPLKEKIEEQVESMTT-ASHDQLASNIENP 660

Query: 661 HVPNTMELEISLDLENDRNVEKADAYSRELEDICNMLRKKHDEAKEMLVRVIVNNSNLLM 720
            VP TMELEISL LENDRNVEKA+AYS++LEDICNMLRKK +EAKE+LVR IVNN+NLLM
Sbjct: 661 RVPKTMELEISLVLENDRNVEKAEAYSQQLEDICNMLRKKQEEAKEILVRAIVNNNNLLM 720

Query: 721 LNHPIFEDKIRKVQKFAAKLLSKELQTKAA 748
           LNHPI+E+KI K+QKFAAK+LSKEL TKAA
Sbjct: 721 LNHPIYEEKIMKLQKFAAKMLSKELHTKAA 739

BLAST of MC01g1462 vs. NCBI nr
Match: XP_023543909.1 (uncharacterized protein At4g18490 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 992 bits (2564), Expect = 0.0
Identity = 548/750 (73.07%), Postives = 606/750 (80.80%), Query Frame = 0

Query: 1   MAESRKGASSATDLRKKDTLLDEDIGDEFMNSWKSISVAEDDM-DFSFGTVSKGKNKAFD 60
           MAE RKGAS ATD RKKD+LLDEDIGDEFMNSWKSISVAEDDM DFSFGTVSKGKNKAFD
Sbjct: 1   MAEPRKGASLATDPRKKDSLLDEDIGDEFMNSWKSISVAEDDMVDFSFGTVSKGKNKAFD 60

Query: 61  FGTLDVDFNLDGSFEKLSSFKMDMSDLEFSCSPKKTEKARSSGKEESPNENLQKDIDSLN 120
           FGTLDVDFNLDGSF+KLSSFK+DM DL+FS  PKK EKARSSGKE SP ENLQ+D+DSLN
Sbjct: 61  FGTLDVDFNLDGSFKKLSSFKIDMPDLDFSSPPKKNEKARSSGKEGSPKENLQRDMDSLN 120

Query: 121 FSFDFKELDSFDVGKSLQNGERSCNRKQDTRAVCSSRVDLAASNIHIAEENTAIDN-SIT 180
           FSFDFKELDSFDV KSLQNGER+C ++QDT AV SSRV+  A NIHI EENTA DN SI 
Sbjct: 121 FSFDFKELDSFDVDKSLQNGERTCKQQQDTEAVSSSRVEHEAFNIHIGEENTATDNTSIA 180

Query: 181 KILPASGKETSSKVQNFQGGRGELESEVVDGTSHEAINAVQTTNKEEQLEKGCLSEKEVS 240
           K LPASG ETSS+V+ F G  GELESEV +GTSHEA N    TN     EKGCL EKE+S
Sbjct: 181 KTLPASGNETSSRVEIFHGDHGELESEVANGTSHEARNTAPATN-----EKGCLPEKELS 240

Query: 241 KSSNLAIHDVPIKCIARNYAPECTSEPQPDIRTTSGELRVVSGVTENVTDEIIDSDVTCC 300
           K S+  IHDVP++CIARNYAPECTSEPQ +I    GEL VVSG T  V DEIIDSDV CC
Sbjct: 241 KGSHQVIHDVPVRCIARNYAPECTSEPQSEICAEKGELTVVSGGTGKVIDEIIDSDVACC 300

Query: 301 KNLPQKDLSPIILCAPESNHTEEDKSECSTPNEVVDKIQLAEVRLDLRDRSNSDVQRKLL 360
           K LP   LSP+ L A E N TE++KSE S  N VVD +QLAEV L L+D SNSD  RKLL
Sbjct: 301 KKLPHSYLSPVNLSATERNPTEKNKSEYSHLNAVVDNVQLAEVHLVLKDCSNSDAPRKLL 360

Query: 361 PDPQEIRENRNLNLKLPTIPLCRGTPVRKVTVKEREIDGNSSTSRTDVVSKPQLHQSSPI 420
            D QEIREN NL LK   +PLCRG PV +VTVKE+E+ GN S S+TDVV K QL+Q+S I
Sbjct: 361 LDTQEIRENHNLKLKPSMVPLCRGLPVNEVTVKEKEVRGNYSISKTDVVRKSQLYQASSI 420

Query: 421 STKLFTLGKNRIDVSNQTPATGDGKLCRDSQPHNKVANTIPPVVVQSEKSYGKLFGLSYS 480
           STKLFTLGKNRID  NQ PA GD   CRD +P NK+ANT PPVVVQSEKS GKL     +
Sbjct: 421 STKLFTLGKNRIDAPNQIPAAGDRNRCRDPRPQNKLANTAPPVVVQSEKSVGKL----RA 480

Query: 481 YSARVTPSNSCVKTTTQTHCNSELLKLSTVPSQSLKIISAQESKLRSIKSSLIFPNLSSL 540
            S RV P N C KTTTQ HCN EL KLS +P++S K ISAQESKL S+K+ LIFP LSSL
Sbjct: 481 SSPRVKPGNLCGKTTTQAHCNPELWKLSMIPNRSAKTISAQESKLCSLKTGLIFPGLSSL 540

Query: 541 KTSWAFGGKQILSSTGREKERKSGDSEQTTEAGQRSKRLDIGYCTENAEKQNLLISNMKR 600
           KT  AFGGKQ+ +STG  KERK G+SEQTTEAGQRSK+LDIGYCTENAEKQ LLISNMKR
Sbjct: 541 KTYRAFGGKQVPTSTGTVKERKLGESEQTTEAGQRSKKLDIGYCTENAEKQELLISNMKR 600

Query: 601 KALEESNADSMLLKPLKRLSVSPIGFRNSKEPLEKKIEEQVEGMTSNASHDHIASSIENP 660
           KALEE NAD   LKPLK L VSP G RN+KEPL+KKIEEQVE MT+ ASHD +AS+IE P
Sbjct: 601 KALEEPNADPKGLKPLKILCVSPNGSRNTKEPLKKKIEEQVESMTT-ASHDQLASNIEKP 660

Query: 661 HVPNTMELEISLDLENDRNVEKADAYSRELEDICNMLRKKHDEAKEMLVRVIVNNSNLLM 720
            VP TMELEISL LENDRNVEKA+AYS++LEDICNMLRKK +EAKE+LVR IVNN+NLLM
Sbjct: 661 RVPETMELEISLVLENDRNVEKAEAYSQQLEDICNMLRKKQEEAKEILVRTIVNNNNLLM 720

Query: 721 LNHPIFEDKIRKVQKFAAKLLSKELQTKAA 748
           LNHPI+E+KI K+QKF+AK+LSKEL TKAA
Sbjct: 721 LNHPIYEEKIMKLQKFSAKMLSKELHTKAA 740

BLAST of MC01g1462 vs. ExPASy TrEMBL
Match: A0A6J1CMB9 (uncharacterized protein At4g18490 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111012291 PE=4 SV=1)

HSP 1 Score: 1330 bits (3443), Expect = 0.0
Identity = 709/748 (94.79%), Postives = 709/748 (94.79%), Query Frame = 0

Query: 1   MAESRKGASSATDLRKKDTLLDEDIGDEFMNSWKSISVAEDDMDFSFGTVSKGKNKAFDF 60
           MAESRKGASSATDLRKKDTLLDEDIGDEFMNSWKSISVAEDDMDFSFGTVSK KNKAFDF
Sbjct: 1   MAESRKGASSATDLRKKDTLLDEDIGDEFMNSWKSISVAEDDMDFSFGTVSKAKNKAFDF 60

Query: 61  GTLDVDFNLDGSFEKLSSFKMDMSDLEFSCSPKKTEKARSSGKEESPNENLQKDIDSLNF 120
           GTLDVDFNLDGSFEKLSSFKMDMSDLEFSCSPKKTEKARSSGKEESPNENLQKDIDSLNF
Sbjct: 61  GTLDVDFNLDGSFEKLSSFKMDMSDLEFSCSPKKTEKARSSGKEESPNENLQKDIDSLNF 120

Query: 121 SFDFKELDSFDVGKSLQNGERSCNRKQDTRAVCSSRVDLAASNIHIAEENTAIDNSITKI 180
           SFDFKELDSFDVGKSLQNGERSCNRKQDTRAVCSSRVDLAASNIHIAEENTAIDNSITKI
Sbjct: 121 SFDFKELDSFDVGKSLQNGERSCNRKQDTRAVCSSRVDLAASNIHIAEENTAIDNSITKI 180

Query: 181 LPASGKETSSKVQNFQGGRGELESEVVDGTSHEAINAVQTTNKEEQLEKGCLSEKEVSKS 240
           LPASGKETSSKVQNFQGGRGELESEVVDGTSHEAINAVQTTNKEEQLEKGCLSEKEVSKS
Sbjct: 181 LPASGKETSSKVQNFQGGRGELESEVVDGTSHEAINAVQTTNKEEQLEKGCLSEKEVSKS 240

Query: 241 SNLAIHDVPIKCIARNYAPECTSEPQPDIRTTSGELRVVSGVTENVTDEIIDSDVTCCKN 300
           SNLAIHDVPIKCIARNYAPECTSEPQPDIRTTSGELRVVSGVTENVTDEIIDSDVTCCKN
Sbjct: 241 SNLAIHDVPIKCIARNYAPECTSEPQPDIRTTSGELRVVSGVTENVTDEIIDSDVTCCKN 300

Query: 301 LPQKDLSPIILCAPESNHTEEDKSECSTPNEVVDKIQLAEVRLDLRDRSNSDVQRKLLPD 360
           LPQKDLSPIILCAPESNHTEEDKSECSTPNEVVDKIQLAEVRLDLRDRSNSDVQRKLLPD
Sbjct: 301 LPQKDLSPIILCAPESNHTEEDKSECSTPNEVVDKIQLAEVRLDLRDRSNSDVQRKLLPD 360

Query: 361 PQEIRENRNLNLKLPTIPLCRGTPVRKVTVKEREIDGNSSTSRTDVVSKPQLHQSSPIST 420
           PQEIRENRNLNLKLPTIPLCRGTPVRKVTVKEREIDGNSSTSRTDVVSKPQLHQSSPIST
Sbjct: 361 PQEIRENRNLNLKLPTIPLCRGTPVRKVTVKEREIDGNSSTSRTDVVSKPQLHQSSPIST 420

Query: 421 KLFTLGKNRIDVSNQTPATGDGKLCRDSQPHNKVANTIPPVVVQSEKSYGKLFGLSYSYS 480
           KLFTLGKNRIDVSNQTPATGDG                                      
Sbjct: 421 KLFTLGKNRIDVSNQTPATGDG-------------------------------------- 480

Query: 481 ARVTPSNSCVKTTTQTHCNSELLKLSTVPSQSLKIISAQESKLRSIKSSLIFPNLSSLKT 540
           ARVTPSNSCVKTTTQTHCNSELLKLSTVPSQSLKIISAQESKLRSIKSSLIFPNLSSLKT
Sbjct: 481 ARVTPSNSCVKTTTQTHCNSELLKLSTVPSQSLKIISAQESKLRSIKSSLIFPNLSSLKT 540

Query: 541 SWAFGGKQILSSTGREKERKSGDSEQTTEAGQRSKRLDIGYCTENAEKQNLLISNMKRKA 600
           SWAFGGKQILSSTGREKERKSGDSEQTTEAGQRSKRLDIGYCTENAEKQNLLISNMKRKA
Sbjct: 541 SWAFGGKQILSSTGREKERKSGDSEQTTEAGQRSKRLDIGYCTENAEKQNLLISNMKRKA 600

Query: 601 LEESNADSMLLKPLKRLSVSPIGFRNSKEPLEKKIEEQVEGMTSNASHDHIASSIENPHV 660
           LEESNADSMLLKPLKRLSVSPIGFRNSKEPLEKKIEEQVEGMTSNASHDHIASSIENPHV
Sbjct: 601 LEESNADSMLLKPLKRLSVSPIGFRNSKEPLEKKIEEQVEGMTSNASHDHIASSIENPHV 660

Query: 661 PNTMELEISLDLENDRNVEKADAYSRELEDICNMLRKKHDEAKEMLVRVIVNNSNLLMLN 720
           PNTMELEISLDLENDRNVEKADAYSRELEDICNMLRKKHDEAKEMLVRVIVNNSNLLMLN
Sbjct: 661 PNTMELEISLDLENDRNVEKADAYSRELEDICNMLRKKHDEAKEMLVRVIVNNSNLLMLN 710

Query: 721 HPIFEDKIRKVQKFAAKLLSKELQTKAA 748
           HPIFEDKIRKVQKFAAKLLSKELQTKAA
Sbjct: 721 HPIFEDKIRKVQKFAAKLLSKELQTKAA 710

BLAST of MC01g1462 vs. ExPASy TrEMBL
Match: A0A6J1CKJ5 (uncharacterized protein At4g18490 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111012291 PE=4 SV=1)

HSP 1 Score: 1214 bits (3140), Expect = 0.0
Identity = 648/687 (94.32%), Postives = 648/687 (94.32%), Query Frame = 0

Query: 62  TLDVDFNLDGSFEKLSSFKMDMSDLEFSCSPKKTEKARSSGKEESPNENLQKDIDSLNFS 121
           T DVDFNLDGSFEKLSSFKMDMSDLEFSCSPKKTEKARSSGKEESPNENLQKDIDSLNFS
Sbjct: 2   TWDVDFNLDGSFEKLSSFKMDMSDLEFSCSPKKTEKARSSGKEESPNENLQKDIDSLNFS 61

Query: 122 FDFKELDSFDVGKSLQNGERSCNRKQDTRAVCSSRVDLAASNIHIAEENTAIDNSITKIL 181
           FDFKELDSFDVGKSLQNGERSCNRKQDTRAVCSSRVDLAASNIHIAEENTAIDNSITKIL
Sbjct: 62  FDFKELDSFDVGKSLQNGERSCNRKQDTRAVCSSRVDLAASNIHIAEENTAIDNSITKIL 121

Query: 182 PASGKETSSKVQNFQGGRGELESEVVDGTSHEAINAVQTTNKEEQLEKGCLSEKEVSKSS 241
           PASGKETSSKVQNFQGGRGELESEVVDGTSHEAINAVQTTNKEEQLEKGCLSEKEVSKSS
Sbjct: 122 PASGKETSSKVQNFQGGRGELESEVVDGTSHEAINAVQTTNKEEQLEKGCLSEKEVSKSS 181

Query: 242 NLAIHDVPIKCIARNYAPECTSEPQPDIRTTSGELRVVSGVTENVTDEIIDSDVTCCKNL 301
           NLAIHDVPIKCIARNYAPECTSEPQPDIRTTSGELRVVSGVTENVTDEIIDSDVTCCKNL
Sbjct: 182 NLAIHDVPIKCIARNYAPECTSEPQPDIRTTSGELRVVSGVTENVTDEIIDSDVTCCKNL 241

Query: 302 PQKDLSPIILCAPESNHTEEDKSECSTPNEVVDKIQLAEVRLDLRDRSNSDVQRKLLPDP 361
           PQKDLSPIILCAPESNHTEEDKSECSTPNEVVDKIQLAEVRLDLRDRSNSDVQRKLLPDP
Sbjct: 242 PQKDLSPIILCAPESNHTEEDKSECSTPNEVVDKIQLAEVRLDLRDRSNSDVQRKLLPDP 301

Query: 362 QEIRENRNLNLKLPTIPLCRGTPVRKVTVKEREIDGNSSTSRTDVVSKPQLHQSSPISTK 421
           QEIRENRNLNLKLPTIPLCRGTPVRKVTVKEREIDGNSSTSRTDVVSKPQLHQSSPISTK
Sbjct: 302 QEIRENRNLNLKLPTIPLCRGTPVRKVTVKEREIDGNSSTSRTDVVSKPQLHQSSPISTK 361

Query: 422 LFTLGKNRIDVSNQTPATGDGKLCRDSQPHNKVANTIPPVVVQSEKSYGKLFGLSYSYSA 481
           LFTLGKNRIDVSNQTPATGDG                                      A
Sbjct: 362 LFTLGKNRIDVSNQTPATGDG--------------------------------------A 421

Query: 482 RVTPSNSCVKTTTQTHCNSELLKLSTVPSQSLKIISAQESKLRSIKSSLIFPNLSSLKTS 541
           RVTPSNSCVKTTTQTHCNSELLKLSTVPSQSLKIISAQESKLRSIKSSLIFPNLSSLKTS
Sbjct: 422 RVTPSNSCVKTTTQTHCNSELLKLSTVPSQSLKIISAQESKLRSIKSSLIFPNLSSLKTS 481

Query: 542 WAFGGKQILSSTGREKERKSGDSEQTTEAGQRSKRLDIGYCTENAEKQNLLISNMKRKAL 601
           WAFGGKQILSSTGREKERKSGDSEQTTEAGQRSKRLDIGYCTENAEKQNLLISNMKRKAL
Sbjct: 482 WAFGGKQILSSTGREKERKSGDSEQTTEAGQRSKRLDIGYCTENAEKQNLLISNMKRKAL 541

Query: 602 EESNADSMLLKPLKRLSVSPIGFRNSKEPLEKKIEEQVEGMTSNASHDHIASSIENPHVP 661
           EESNADSMLLKPLKRLSVSPIGFRNSKEPLEKKIEEQVEGMTSNASHDHIASSIENPHVP
Sbjct: 542 EESNADSMLLKPLKRLSVSPIGFRNSKEPLEKKIEEQVEGMTSNASHDHIASSIENPHVP 601

Query: 662 NTMELEISLDLENDRNVEKADAYSRELEDICNMLRKKHDEAKEMLVRVIVNNSNLLMLNH 721
           NTMELEISLDLENDRNVEKADAYSRELEDICNMLRKKHDEAKEMLVRVIVNNSNLLMLNH
Sbjct: 602 NTMELEISLDLENDRNVEKADAYSRELEDICNMLRKKHDEAKEMLVRVIVNNSNLLMLNH 650

Query: 722 PIFEDKIRKVQKFAAKLLSKELQTKAA 748
           PIFEDKIRKVQKFAAKLLSKELQTKAA
Sbjct: 662 PIFEDKIRKVQKFAAKLLSKELQTKAA 650

BLAST of MC01g1462 vs. ExPASy TrEMBL
Match: A0A6J1CJW4 (uncharacterized protein At4g18490 isoform X3 OS=Momordica charantia OX=3673 GN=LOC111012291 PE=4 SV=1)

HSP 1 Score: 1180 bits (3052), Expect = 0.0
Identity = 630/668 (94.31%), Postives = 630/668 (94.31%), Query Frame = 0

Query: 81  MDMSDLEFSCSPKKTEKARSSGKEESPNENLQKDIDSLNFSFDFKELDSFDVGKSLQNGE 140
           MDMSDLEFSCSPKKTEKARSSGKEESPNENLQKDIDSLNFSFDFKELDSFDVGKSLQNGE
Sbjct: 1   MDMSDLEFSCSPKKTEKARSSGKEESPNENLQKDIDSLNFSFDFKELDSFDVGKSLQNGE 60

Query: 141 RSCNRKQDTRAVCSSRVDLAASNIHIAEENTAIDNSITKILPASGKETSSKVQNFQGGRG 200
           RSCNRKQDTRAVCSSRVDLAASNIHIAEENTAIDNSITKILPASGKETSSKVQNFQGGRG
Sbjct: 61  RSCNRKQDTRAVCSSRVDLAASNIHIAEENTAIDNSITKILPASGKETSSKVQNFQGGRG 120

Query: 201 ELESEVVDGTSHEAINAVQTTNKEEQLEKGCLSEKEVSKSSNLAIHDVPIKCIARNYAPE 260
           ELESEVVDGTSHEAINAVQTTNKEEQLEKGCLSEKEVSKSSNLAIHDVPIKCIARNYAPE
Sbjct: 121 ELESEVVDGTSHEAINAVQTTNKEEQLEKGCLSEKEVSKSSNLAIHDVPIKCIARNYAPE 180

Query: 261 CTSEPQPDIRTTSGELRVVSGVTENVTDEIIDSDVTCCKNLPQKDLSPIILCAPESNHTE 320
           CTSEPQPDIRTTSGELRVVSGVTENVTDEIIDSDVTCCKNLPQKDLSPIILCAPESNHTE
Sbjct: 181 CTSEPQPDIRTTSGELRVVSGVTENVTDEIIDSDVTCCKNLPQKDLSPIILCAPESNHTE 240

Query: 321 EDKSECSTPNEVVDKIQLAEVRLDLRDRSNSDVQRKLLPDPQEIRENRNLNLKLPTIPLC 380
           EDKSECSTPNEVVDKIQLAEVRLDLRDRSNSDVQRKLLPDPQEIRENRNLNLKLPTIPLC
Sbjct: 241 EDKSECSTPNEVVDKIQLAEVRLDLRDRSNSDVQRKLLPDPQEIRENRNLNLKLPTIPLC 300

Query: 381 RGTPVRKVTVKEREIDGNSSTSRTDVVSKPQLHQSSPISTKLFTLGKNRIDVSNQTPATG 440
           RGTPVRKVTVKEREIDGNSSTSRTDVVSKPQLHQSSPISTKLFTLGKNRIDVSNQTPATG
Sbjct: 301 RGTPVRKVTVKEREIDGNSSTSRTDVVSKPQLHQSSPISTKLFTLGKNRIDVSNQTPATG 360

Query: 441 DGKLCRDSQPHNKVANTIPPVVVQSEKSYGKLFGLSYSYSARVTPSNSCVKTTTQTHCNS 500
           DG                                      ARVTPSNSCVKTTTQTHCNS
Sbjct: 361 DG--------------------------------------ARVTPSNSCVKTTTQTHCNS 420

Query: 501 ELLKLSTVPSQSLKIISAQESKLRSIKSSLIFPNLSSLKTSWAFGGKQILSSTGREKERK 560
           ELLKLSTVPSQSLKIISAQESKLRSIKSSLIFPNLSSLKTSWAFGGKQILSSTGREKERK
Sbjct: 421 ELLKLSTVPSQSLKIISAQESKLRSIKSSLIFPNLSSLKTSWAFGGKQILSSTGREKERK 480

Query: 561 SGDSEQTTEAGQRSKRLDIGYCTENAEKQNLLISNMKRKALEESNADSMLLKPLKRLSVS 620
           SGDSEQTTEAGQRSKRLDIGYCTENAEKQNLLISNMKRKALEESNADSMLLKPLKRLSVS
Sbjct: 481 SGDSEQTTEAGQRSKRLDIGYCTENAEKQNLLISNMKRKALEESNADSMLLKPLKRLSVS 540

Query: 621 PIGFRNSKEPLEKKIEEQVEGMTSNASHDHIASSIENPHVPNTMELEISLDLENDRNVEK 680
           PIGFRNSKEPLEKKIEEQVEGMTSNASHDHIASSIENPHVPNTMELEISLDLENDRNVEK
Sbjct: 541 PIGFRNSKEPLEKKIEEQVEGMTSNASHDHIASSIENPHVPNTMELEISLDLENDRNVEK 600

Query: 681 ADAYSRELEDICNMLRKKHDEAKEMLVRVIVNNSNLLMLNHPIFEDKIRKVQKFAAKLLS 740
           ADAYSRELEDICNMLRKKHDEAKEMLVRVIVNNSNLLMLNHPIFEDKIRKVQKFAAKLLS
Sbjct: 601 ADAYSRELEDICNMLRKKHDEAKEMLVRVIVNNSNLLMLNHPIFEDKIRKVQKFAAKLLS 630

Query: 741 KELQTKAA 748
           KELQTKAA
Sbjct: 661 KELQTKAA 630

BLAST of MC01g1462 vs. ExPASy TrEMBL
Match: A0A6J1EJB0 (uncharacterized protein At4g18490 OS=Cucurbita moschata OX=3662 GN=LOC111433117 PE=4 SV=1)

HSP 1 Score: 998 bits (2580), Expect = 0.0
Identity = 554/750 (73.87%), Postives = 609/750 (81.20%), Query Frame = 0

Query: 1   MAESRKGASSATDLRKKDTLLDEDIGDEFMNSWKSISVAEDDM-DFSFGTVSKGKNKAFD 60
           MAE RKGAS ATD RKKD+LLDEDIGDEFMNSWKSISVAEDDM DFSFGTVSKGKNKAFD
Sbjct: 1   MAEPRKGASLATDPRKKDSLLDEDIGDEFMNSWKSISVAEDDMVDFSFGTVSKGKNKAFD 60

Query: 61  FGTLDVDFNLDGSFEKLSSFKMDMSDLEFSCSPKKTEKARSSGKEESPNENLQKDIDSLN 120
           FGTLDVDFNLDGSFEKLSSFK+DM DL+FS  PKK EKARSSGKE SP ENLQ+D+DSLN
Sbjct: 61  FGTLDVDFNLDGSFEKLSSFKIDMPDLDFSSPPKKNEKARSSGKEGSPKENLQRDMDSLN 120

Query: 121 FSFDFKELDSFDVGKSLQNGERSCNRKQDTRAVCSSRVDLAASNIHIAEENTAIDN-SIT 180
           FSFDFKELDSFDV KSLQNGER+C ++QDT AV SSRV+  A NIHI EENTA DN SI 
Sbjct: 121 FSFDFKELDSFDVDKSLQNGERTCKQQQDTEAVSSSRVEHEAFNIHIGEENTATDNTSIA 180

Query: 181 KILPASGKETSSKVQNFQGGRGELESEVVDGTSHEAINAVQTTNKEEQLEKGCLSEKEVS 240
           K LPASG ETSS+V+NF G  GELESEV DGTSHEA N    TN     EKGCL EKE+S
Sbjct: 181 KRLPASGNETSSRVENFHGDHGELESEVADGTSHEARNTAPATN-----EKGCLPEKELS 240

Query: 241 KSSNLAIHDVPIKCIARNYAPECTSEPQPDIRTTSGELRVVSGVTENVTDEIIDSDVTCC 300
           K S+  IHDVP++CIARNYAPECTSEPQ +I    GEL VVSG T  VTDEIIDSD  CC
Sbjct: 241 KGSHQVIHDVPVRCIARNYAPECTSEPQSEICAEKGELTVVSGGTGKVTDEIIDSDGACC 300

Query: 301 KNLPQKDLSPIILCAPESNHTEEDKSECSTPNEVVDKIQLAEVRLDLRDRSNSDVQRKLL 360
           K LP   LS I L A E N TE++KSE S  NEVVD +QLAEV L L+D SNSD  RKLL
Sbjct: 301 KKLPHSYLSSINLSASERNPTEKNKSEYSHLNEVVDNVQLAEVHLVLKDCSNSDAPRKLL 360

Query: 361 PDPQEIRENRNLNLKLPTIPLCRGTPVRKVTVKEREIDGNSSTSRTDVVSKPQLHQSSPI 420
            D QEIREN N  LK   +PLCRG PV +VTVKE+E+ GN S S+TDVV K QL+Q+S I
Sbjct: 361 LDTQEIRENHNSKLKPSIVPLCRGLPVNEVTVKEKEVSGNYSISKTDVVRKSQLYQASFI 420

Query: 421 STKLFTLGKNRIDVSNQTPATGDGKLCRDSQPHNKVANTIPPVVVQSEKSYGKLFGLSYS 480
           STKLFTLGKNRID  NQ PA GD   CRDS+P NK+ANT PPVVVQSEKS GKL     +
Sbjct: 421 STKLFTLGKNRIDAPNQIPAAGDRNRCRDSRPQNKLANTAPPVVVQSEKSVGKL----RA 480

Query: 481 YSARVTPSNSCVKTTTQTHCNSELLKLSTVPSQSLKIISAQESKLRSIKSSLIFPNLSSL 540
            S RV PSN C KTTTQ HCN EL KLS +P++S K ISAQESKL S+K+ LIFP LSSL
Sbjct: 481 SSPRVKPSNLCGKTTTQAHCNPELWKLSMIPNRSAKTISAQESKLCSLKTGLIFPGLSSL 540

Query: 541 KTSWAFGGKQILSSTGREKERKSGDSEQTTEAGQRSKRLDIGYCTENAEKQNLLISNMKR 600
           KT  AFGGKQ+ +STG  KERK G+SEQT EAGQRSK+LDIGYCTENAEKQ LLISNMKR
Sbjct: 541 KTYRAFGGKQVPTSTGTVKERKLGESEQT-EAGQRSKKLDIGYCTENAEKQELLISNMKR 600

Query: 601 KALEESNADSMLLKPLKRLSVSPIGFRNSKEPLEKKIEEQVEGMTSNASHDHIASSIENP 660
           KALEE NAD   LKPLK L VSP GFRN+KEPL++KIEEQVE MT+ ASHD +AS+IENP
Sbjct: 601 KALEEPNADPKGLKPLKHLCVSPSGFRNTKEPLKEKIEEQVESMTT-ASHDQLASNIENP 660

Query: 661 HVPNTMELEISLDLENDRNVEKADAYSRELEDICNMLRKKHDEAKEMLVRVIVNNSNLLM 720
            VP TMELEISL LENDRNVEKA+AYS++LEDICNMLRKK +EAKE+LVR IVNN+NLLM
Sbjct: 661 RVPKTMELEISLVLENDRNVEKAEAYSQQLEDICNMLRKKQEEAKEILVRAIVNNNNLLM 720

Query: 721 LNHPIFEDKIRKVQKFAAKLLSKELQTKAA 748
           LNHPI+E+KI K+QKFAAK+LSKEL TKAA
Sbjct: 721 LNHPIYEEKIMKLQKFAAKMLSKELHTKAA 739

BLAST of MC01g1462 vs. ExPASy TrEMBL
Match: A0A6J1INH0 (uncharacterized protein At4g18490 OS=Cucurbita maxima OX=3661 GN=LOC111478562 PE=4 SV=1)

HSP 1 Score: 981 bits (2537), Expect = 0.0
Identity = 545/750 (72.67%), Postives = 604/750 (80.53%), Query Frame = 0

Query: 1   MAESRKGASSATDLRKKDTLLDEDIGDEFMNSWKSISVAEDDM-DFSFGTVSKGKNKAFD 60
           MAE RKGAS ATD RKKD+LLDEDIGDEFMNSWKSISVAEDDM DFSFGTVSKGKNKAFD
Sbjct: 1   MAEPRKGASLATDPRKKDSLLDEDIGDEFMNSWKSISVAEDDMVDFSFGTVSKGKNKAFD 60

Query: 61  FGTLDVDFNLDGSFEKLSSFKMDMSDLEFSCSPKKTEKARSSGKEESPNENLQKDIDSLN 120
           FGTLDVDFNLDGSFEKLSSFK+DM DL+FS  PKK EKARSS KE SP ENLQ+D+DSLN
Sbjct: 61  FGTLDVDFNLDGSFEKLSSFKIDMPDLDFSSPPKKNEKARSSCKEGSPKENLQRDMDSLN 120

Query: 121 FSFDFKELDSFDVGKSLQNGERSCNRKQDTRAVCSSRVDLAASNIHIAEENTAIDN-SIT 180
           FSFDFKELDSFDV KSLQNGER+C ++QDT AV SSRV+  A NIHI EENTA DN SI 
Sbjct: 121 FSFDFKELDSFDVDKSLQNGERTCKQQQDTEAVSSSRVEHEAFNIHIGEENTATDNTSIA 180

Query: 181 KILPASGKETSSKVQNFQGGRGELESEVVDGTSHEAINAVQTTNKEEQLEKGCLSEKEVS 240
           K LP+SG ETSS+V+NF G  GELESEV DGTSHEA N    TN     EKGCL E E+S
Sbjct: 181 KRLPSSGNETSSRVENFHGDHGELESEVADGTSHEARNTAPATN-----EKGCLPENELS 240

Query: 241 KSSNLAIHDVPIKCIARNYAPECTSEPQPDIRTTSGELRVVSGVTENVTDEIIDSDVTCC 300
           K S+  IHDVP++CIARNYAPECTSEPQ +I    GEL VVSG T  VTDEI+DSDV CC
Sbjct: 241 KGSHQVIHDVPVRCIARNYAPECTSEPQSEICAEKGELTVVSGGTGKVTDEIVDSDVACC 300

Query: 301 KNLPQKDLSPIILCAPESNHTEEDKSECSTPNEVVDKIQLAEVRLDLRDRSNSDVQRKLL 360
           K LP   LSPI L A E N TE++KS+ S  NEVVD +QLAEV L L+D SNSD  RKLL
Sbjct: 301 KKLPHSYLSPIDLSASERNPTEKNKSKYSHLNEVVDNVQLAEVHLVLKDCSNSDAPRKLL 360

Query: 361 PDPQEIRENRNLNLKLPTIPLCRGTPVRKVTVKEREIDGNSSTSRTDVVSKPQLHQSSPI 420
            D QEIREN   ++    +PLCRG  V +VTVKE+E+ GN S S+TDVV K QL+Q+S I
Sbjct: 361 LDRQEIRENHKPSM----VPLCRGLLVNEVTVKEKEVRGNYSISKTDVVRKSQLYQASSI 420

Query: 421 STKLFTLGKNRIDVSNQTPATGDGKLCRDSQPHNKVANTIPPVVVQSEKSYGKLFGLSYS 480
           STKLFTLG NRID  NQ P  GD   CRDS+P NK+ANT PPVVVQSEKS GKL     +
Sbjct: 421 STKLFTLGNNRIDAPNQIPEAGDRNRCRDSRPQNKLANTAPPVVVQSEKSVGKL----RA 480

Query: 481 YSARVTPSNSCVKTTTQTHCNSELLKLSTVPSQSLKIISAQESKLRSIKSSLIFPNLSSL 540
            S RV PSN C KTTTQ HCN EL KLS +P++S K ISAQESKL S+K+ LIFP LSSL
Sbjct: 481 SSPRVKPSNLCGKTTTQAHCNPELWKLSMIPNRSAKTISAQESKLCSLKTGLIFPGLSSL 540

Query: 541 KTSWAFGGKQILSSTGREKERKSGDSEQTTEAGQRSKRLDIGYCTENAEKQNLLISNMKR 600
           KT  AFGGKQ+ +STG  KERK G+SEQTTEAGQRSK+LDIGYCTENAEKQ LLISNMKR
Sbjct: 541 KTYRAFGGKQVPTSTGTVKERKLGESEQTTEAGQRSKKLDIGYCTENAEKQELLISNMKR 600

Query: 601 KALEESNADSMLLKPLKRLSVSPIGFRNSKEPLEKKIEEQVEGMTSNASHDHIASSIENP 660
           KALEE NAD   LKPLK L VSP G RN+KEPL+KKIEEQVE MT+ ASHD + S+IENP
Sbjct: 601 KALEEPNADPKGLKPLKHLCVSPSGSRNTKEPLKKKIEEQVESMTT-ASHDQLGSNIENP 660

Query: 661 HVPNTMELEISLDLENDRNVEKADAYSRELEDICNMLRKKHDEAKEMLVRVIVNNSNLLM 720
            VP TMELEISL LENDRNVEKA+AYS++LEDICNMLRKK +EAKE+LVR IVNN+NLL+
Sbjct: 661 RVPKTMELEISLVLENDRNVEKAEAYSQQLEDICNMLRKKQEEAKEILVRAIVNNNNLLL 720

Query: 721 LNHPIFEDKIRKVQKFAAKLLSKELQTKAA 748
           LNHPI+E+KI K+QKFAAKLLSKELQ KAA
Sbjct: 721 LNHPIYEEKIMKLQKFAAKLLSKELQKKAA 736

BLAST of MC01g1462 vs. TAIR 10
Match: AT4G18490.1 (unknown protein; Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 168.3 bits (425), Expect = 2.3e-41
Identity = 217/777 (27.93%), Postives = 352/777 (45.30%), Query Frame = 0

Query: 10  SATDLRKKDTLLDEDIGDEFMNSWKSISVAEDD-MDFSFGTVSKGKNKAFDFGTLDVDFN 69
           S+TD + KD +L  D  D   ++W   S+ +DD MDF FG+ +K K  AF    LD+ F+
Sbjct: 8   SSTDTQDKDLMLAAD-KDMEKDTWNFKSMTDDDPMDFGFGSPAKNKKNAF---KLDMGFD 67

Query: 70  LDGSFEKLSSFKMDMSDLEFSCSPKKTEKARSSGKEESPNENLQKDIDSLNFSFDFKELD 129
           LDG F   SSFKMDM D +FS   KKT K + +  ++ P+ N ++  +   FS+DF  LD
Sbjct: 68  LDGDFG--SSFKMDMPDFDFSSPAKKTTKTKET-SDDKPSGNSKQKKNPFAFSYDFDALD 127

Query: 130 SFDVGKS-LQNGERSCNRKQDTRAVCSSR-----------VDLAASNIHIAEENTAIDNS 189
            FD+G S  + G ++  +  D   +C+S            +DL  +    ++ NT +   
Sbjct: 128 DFDLGSSPPKKGSKTTTKSMDCEEICASSKSDKSDDLDFGLDLPITRQVPSKANTDVQAK 187

Query: 190 ITKILPASGKETSSKV---QNFQGGRGELES----EVVDGTSHEAINAVQT--------- 249
            +    +   +T+  +   ++    +  LES    E V+        A QT         
Sbjct: 188 ASAEKESQNYKTTDTLVVNKSKNSNQAALESMGDFEAVESPQGSRKKASQTHTMCVQPQS 247

Query: 250 ---------TNKEEQLEKGCLSEKEVSKSSNLAIHDVPIKCIARNYAPECTSEPQPDIR- 309
                     +K E+  + C S + ++ S    +H   I  IA N       E  PDI  
Sbjct: 248 VDTSPLKTSCSKVEEKNEPCPSNETIAPS---PLHASEIAHIAVN------RETSPDIHE 307

Query: 310 -TTSGELRVVSGVTENVTDEIIDSDVTCCKNLPQKDLSPII---LCAPESNHTEEDKSEC 369
              SG         EN   ++I +  +  + + Q   SP I   LC+ +  H +E+    
Sbjct: 308 LCRSGTKEDCPIDPENANKKMITTMESSYEKIEQ--TSPSISSHLCSDKIEHQQEEMG-T 367

Query: 370 STPNEVVDKIQLAEVRLDLRDRSNSDVQRKLLPDPQ--EIRENRNLNLKLPTIPLCRGTP 429
            T  E+ D  + A    D    S + +  K+ P  +  +  + ++L+ KLP  P      
Sbjct: 368 DTQAEIQDNTKGALYNSD-AGHSLTTLSGKISPGTRTSQTAKVQDLSEKLPLDPSHSMAG 427

Query: 430 VRKVTVKEREIDGNSSTSRTDVVSKPQLH--QSSPISTKLFTLGKNRIDVSNQTPATGDG 489
           +  +   + +  G   +       KP+ H  +SSPI T++  + +  I  S+  P     
Sbjct: 428 LDNLRAMQNKDLGLIRSRFFKKPEKPESHVLESSPIETEIQPVIRENIG-SSLNPT---- 487

Query: 490 KLCRDSQPHNKVANTIPPVVVQSEKSYGKLFGLSYSYSARVTPSNSCVKTTTQTHCNSEL 549
               D+  H K+ +         E   G++  L    +   T   S ++    +  ++  
Sbjct: 488 ---NDTSSHEKIIHKDHSNGKTVENVAGQMDHLKLQ-AKNTTREKSILQINISSKLDASS 547

Query: 550 LKLSTVPSQSLKIISAQESKLRSI---KSSLIFPNLSSLKTSWAFGGKQILSSTGREKER 609
           L        S  + S Q+ KL S+   K   I  +L ++KT    GG +   S+  + E 
Sbjct: 548 LTQKLSNHLSSGVESLQKPKLNSLGRPKLGNIMADLRAVKTQRTIGGNKDQPSSAVQPEF 607

Query: 610 KSGDSEQTTEAGQRSKRLDIGYCTENAEKQNL-LISNMKRKALEESNADSMLLKPLKRLS 669
            S  S++        K  +I +     + Q L   S++KRKAL+E +AD  L   LKRLS
Sbjct: 608 SSSISKERNTEAPVKKSSEIHHLAPRDKTQTLHCPSSLKRKALDE-DADRSLKPQLKRLS 667

Query: 670 VSPIGFRNSKEPLEKKIEEQVEGMTSNASHDHIASSI-ENPHVP------NTMELEISLD 729
           +SP   RN +E   + ++ +     S          + E+P         N   LEI + 
Sbjct: 668 MSPRENRNVEELTHRAVQGKFSSQESRIDTKTTKEPVKESPQTKSHYQNINMANLEIPI- 727

BLAST of MC01g1462 vs. TAIR 10
Match: AT4G18490.2 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; EXPRESSED IN: shoot, shoot apex, embryo, flower, seed; EXPRESSED DURING: petal differentiation and expansion stage, E expanded cotyledon stage; Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 167.5 bits (423), Expect = 4.0e-41
Identity = 217/778 (27.89%), Postives = 354/778 (45.50%), Query Frame = 0

Query: 10  SATDLRKKDTLLDEDIGDEFMNSWKSISVAEDD-MDFSFGTVSKGKNKAFDFGTLDVDFN 69
           S+TD + KD +LD+D+  +   +W   S+ +DD MDF FG+ +K K  AF    LD+ F+
Sbjct: 8   SSTDTQDKDLMLDKDMEKD---TWNFKSMTDDDPMDFGFGSPAKNKKNAF---KLDMGFD 67

Query: 70  LDGSFEKLSSFKMDMSDLEFSCSPKKTEKARSSGKEESPNENLQKDIDSLNFSFDF-KEL 129
           LDG F   SSFKMDM D +FS   KKT K + +  ++ P+ N ++  +   FS+DF   L
Sbjct: 68  LDGDFG--SSFKMDMPDFDFSSPAKKTTKTKET-SDDKPSGNSKQKKNPFAFSYDFDARL 127

Query: 130 DSFDVGKS-LQNGERSCNRKQDTRAVCSSR-----------VDLAASNIHIAEENTAIDN 189
           D FD+G S  + G ++  +  D   +C+S            +DL  +    ++ NT +  
Sbjct: 128 DDFDLGSSPPKKGSKTTTKSMDCEEICASSKSDKSDDLDFGLDLPITRQVPSKANTDVQA 187

Query: 190 SITKILPASGKETSSKV---QNFQGGRGELES----EVVDGTSHEAINAVQT-------- 249
             +    +   +T+  +   ++    +  LES    E V+        A QT        
Sbjct: 188 KASAEKESQNYKTTDTLVVNKSKNSNQAALESMGDFEAVESPQGSRKKASQTHTMCVQPQ 247

Query: 250 ----------TNKEEQLEKGCLSEKEVSKSSNLAIHDVPIKCIARNYAPECTSEPQPDIR 309
                      +K E+  + C S + ++ S    +H   I  IA N       E  PDI 
Sbjct: 248 SVDTSPLKTSCSKVEEKNEPCPSNETIAPS---PLHASEIAHIAVN------RETSPDIH 307

Query: 310 --TTSGELRVVSGVTENVTDEIIDSDVTCCKNLPQKDLSPII---LCAPESNHTEEDKSE 369
               SG         EN   ++I +  +  + + Q   SP I   LC+ +  H +E+   
Sbjct: 308 ELCRSGTKEDCPIDPENANKKMITTMESSYEKIEQ--TSPSISSHLCSDKIEHQQEEMG- 367

Query: 370 CSTPNEVVDKIQLAEVRLDLRDRSNSDVQRKLLPDPQ--EIRENRNLNLKLPTIPLCRGT 429
             T  E+ D  + A    D    S + +  K+ P  +  +  + ++L+ KLP  P     
Sbjct: 368 TDTQAEIQDNTKGALYNSD-AGHSLTTLSGKISPGTRTSQTAKVQDLSEKLPLDPSHSMA 427

Query: 430 PVRKVTVKEREIDGNSSTSRTDVVSKPQLH--QSSPISTKLFTLGKNRIDVSNQTPATGD 489
            +  +   + +  G   +       KP+ H  +SSPI T++  + +  I  S+  P    
Sbjct: 428 GLDNLRAMQNKDLGLIRSRFFKKPEKPESHVLESSPIETEIQPVIRENIG-SSLNPT--- 487

Query: 490 GKLCRDSQPHNKVANTIPPVVVQSEKSYGKLFGLSYSYSARVTPSNSCVKTTTQTHCNSE 549
                D+  H K+ +         E   G++  L    +   T   S ++    +  ++ 
Sbjct: 488 ----NDTSSHEKIIHKDHSNGKTVENVAGQMDHLKLQ-AKNTTREKSILQINISSKLDAS 547

Query: 550 LLKLSTVPSQSLKIISAQESKLRSI---KSSLIFPNLSSLKTSWAFGGKQILSSTGREKE 609
            L        S  + S Q+ KL S+   K   I  +L ++KT    GG +   S+  + E
Sbjct: 548 SLTQKLSNHLSSGVESLQKPKLNSLGRPKLGNIMADLRAVKTQRTIGGNKDQPSSAVQPE 607

Query: 610 RKSGDSEQTTEAGQRSKRLDIGYCTENAEKQNL-LISNMKRKALEESNADSMLLKPLKRL 669
             S  S++        K  +I +     + Q L   S++KRKAL+E +AD  L   LKRL
Sbjct: 608 FSSSISKERNTEAPVKKSSEIHHLAPRDKTQTLHCPSSLKRKALDE-DADRSLKPQLKRL 667

Query: 670 SVSPIGFRNSKEPLEKKIEEQVEGMTSNASHDHIASSI-ENPHVP------NTMELEISL 729
           S+SP   RN +E   + ++ +     S          + E+P         N   LEI +
Sbjct: 668 SMSPRENRNVEELTHRAVQGKFSSQESRIDTKTTKEPVKESPQTKSHYQNINMANLEIPI 727