MC01g1126 (gene) Bitter gourd (Dali-11) v1

BLAST of MC01g1126 vs. NCBI nr
Match: XP_038882287.1 (uncharacterized protein LOC120073519 [Benincasa hispida])

HSP 1 Score: 276 bits (705), Expect = 1.73e-90
Identity = 152/232 (65.52%), Postives = 177/232 (76.29%), Query Frame = 0

Query: 1   MKTEEIGAMGVCGNLRILVKIFRFTEVFVGLAFFFWIFNRLPLAVRVSSDYISQISGFFS 60
           MKTE++ AMGV   L+I+VKIFRFTEV V + F FW F+RLPL +RVS DYISQIS +F+
Sbjct: 1   MKTEKVKAMGVYNLLQIMVKIFRFTEVLVAMGFLFWTFSRLPLVLRVSKDYISQISNYFT 60

Query: 61  SSVFGFVLCNTIVVALIANPDRFSGQKLAGDSVETEIFNELVENGAGSSLSLPGNEPIGR 120
             VFGF+LCNTI+VALIA P  FSG K++ D+VETE+FNELV++GA  SLSLPG+EPIGR
Sbjct: 61  GPVFGFILCNTIIVALIAKP-HFSGGKISSDNVETELFNELVKDGADRSLSLPGSEPIGR 120

Query: 121 LCSDPEKEEIVYEDKEVISEVSSAAALI-----ENMDGSSPGLGCLMPENVGEKLRRLAV 180
           LCSD  +EEI YEDKEVISEV S  A I     ++    S  L     E  G + RRL  
Sbjct: 121 LCSDSGQEEIAYEDKEVISEVRSMKAAIFTEKRDDFGSESEKLKLAWMEFSGGEFRRLEA 180

Query: 181 EEKEAIWGGGISVESVWYGEELSNKEFQERIEAFIARELRFRREESGAVVLR 227
           EE EAIWGGG S ESVW+GEELSNKEFQ RI+AFIARELRFR EESGAV+LR
Sbjct: 181 EE-EAIWGGGFSPESVWHGEELSNKEFQRRIDAFIARELRFRWEESGAVILR 230

BLAST of MC01g1126 vs. NCBI nr
Match: XP_022977278.1 (uncharacterized protein LOC111477644 [Cucurbita maxima])

HSP 1 Score: 273 bits (697), Expect = 3.59e-89
Identity = 153/231 (66.23%), Postives = 178/231 (77.06%), Query Frame = 0

Query: 1   MKTEEIGAMGVCGNLRILVKIFRFTEVFVGLAFFFWIFNRLPLAVRVSSDYISQISGFFS 60
           MKTEE+ AMG+  +LR L+K FRFTEVF  +A   W F+RLPL +RVSSDYISQISG+F+
Sbjct: 1   MKTEEVEAMGLSDHLRTLLKFFRFTEVFAAMALLVWTFSRLPLILRVSSDYISQISGYFT 60

Query: 61  SSVFGFVLCNTIVVALIANPDRFSGQKLAGDSVETEIFNELVENGAGSSLSLPGNEPIGR 120
           S VFGF+LCNTI++ALIA P RFSG+K  GD+VETE+ NELV++GA +SLSLPG+EP GR
Sbjct: 61  SPVFGFMLCNTIIIALIAKPHRFSGEKFTGDNVETELVNELVKDGALTSLSLPGSEPNGR 120

Query: 121 LCSDPEKEEIVYEDKEVISEVSS--AAALIENMDGSSPG---LGCLMPENVGEKLRRLAV 180
           LCSD E EEI YEDKEVISEVSS  AAA  E ++  S G   L     E  G +  R  V
Sbjct: 121 LCSDVELEEIAYEDKEVISEVSSTTAAAFTEEIEDFSSGSEKLKLAWSEFGGGEFERAEV 180

Query: 181 EEKEAIWGGGISVESVWYGEELSNKEFQERIEAFIARELRFRREESGAVVL 226
           EE EAIWGGG+  ESV  GEELSNKEF+ RI+AFIARELRFR EESGAVVL
Sbjct: 181 EE-EAIWGGGVLPESVRQGEELSNKEFERRIDAFIARELRFRWEESGAVVL 230

BLAST of MC01g1126 vs. NCBI nr
Match: KAG6603959.1 (hypothetical protein SDJN03_04568, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 268 bits (686), Expect = 1.68e-87
Identity = 151/231 (65.37%), Postives = 177/231 (76.62%), Query Frame = 0

Query: 1   MKTEEIGAMGVCGNLRILVKIFRFTEVFVGLAFFFWIFNRLPLAVRVSSDYISQISGFFS 60
           MKTEE+ AMG+  +LR L+K FR TEVF  +AF  W F+RLPL +RVSSDYISQISG+F+
Sbjct: 1   MKTEEVEAMGLSDHLRTLLKFFRLTEVFAAMAFLVWTFSRLPLILRVSSDYISQISGYFT 60

Query: 61  SSVFGFVLCNTIVVALIANPDRFSGQKLAGDSVETEIFNELVENGAGSSLSLPGNEPIGR 120
           S +FGF+LCNTI++ALI+ P RFSG K  GD+VETE+ NELV++GA +SLSLPG+EP GR
Sbjct: 61  SPIFGFMLCNTIIIALISKPHRFSGGKFTGDNVETELVNELVKDGALTSLSLPGSEPNGR 120

Query: 121 LCSDPEKEEIVYEDKEVISEVSS--AAALIENMDGSSPG---LGCLMPENVGEKLRRLAV 180
           LCSD E EEI YEDKEVISEVSS  AAA  E ++  S G   L     E  G +  R  V
Sbjct: 121 LCSDLELEEIAYEDKEVISEVSSTTAAAFTEEIEDFSSGSEKLKLAWSEFGGGEFGRAEV 180

Query: 181 EEKEAIWGGGISVESVWYGEELSNKEFQERIEAFIARELRFRREESGAVVL 226
           EE EAIWGGG+  ESV  GEELSNKEF+ RI+AFIARELRFR EESGAVVL
Sbjct: 181 EE-EAIWGGGVLPESVRQGEELSNKEFERRIDAFIARELRFRWEESGAVVL 230

BLAST of MC01g1126 vs. NCBI nr
Match: XP_022950349.1 (uncharacterized protein LOC111453461 [Cucurbita moschata])

HSP 1 Score: 264 bits (674), Expect = 1.12e-85
Identity = 149/231 (64.50%), Postives = 172/231 (74.46%), Query Frame = 0

Query: 1   MKTEEIGAMGVCGNLRILVKIFRFTEVFVGLAFFFWIFNRLPLAVRVSSDYISQISGFFS 60
           MKTEE+ AMG+  +LR L+K  RFTEVF  +AF  W F+RLPL +RVSSDYISQISG+F+
Sbjct: 1   MKTEEVEAMGLSDHLRTLLKFLRFTEVFAAMAFLVWTFSRLPLILRVSSDYISQISGYFT 60

Query: 61  SSVFGFVLCNTIVVALIANPDRFSGQKLAGDSVETEIFNELVENGAGSSLSLPGNEPIGR 120
           S VFGF+LCNTI++ALIA P RFSG K  GD+VETE+ NELV++GA +SLSLPG+ P GR
Sbjct: 61  SPVFGFMLCNTIIIALIAKPPRFSGGKFTGDNVETELVNELVKDGALTSLSLPGSGPNGR 120

Query: 121 LCSDPEKEEIVYEDKEVISEVSSAAAL-----IENMDGSSPGLGCLMPENVGEKLRRLAV 180
           LCSD E EEI YEDKEVISEVSS  A      IE+    S  L     E  G +  R  V
Sbjct: 121 LCSDLELEEIAYEDKEVISEVSSTTAAAFTEEIEDFCSGSEKLKLAWSEFGGGEFGRAEV 180

Query: 181 EEKEAIWGGGISVESVWYGEELSNKEFQERIEAFIARELRFRREESGAVVL 226
           E  EAIWGGG+  ESV  GEELSNKEF+ RI+AFIARELRFR EESGAVVL
Sbjct: 181 EA-EAIWGGGVLPESVRQGEELSNKEFERRIDAFIARELRFRWEESGAVVL 230

BLAST of MC01g1126 vs. NCBI nr
Match: XP_023543998.1 (uncharacterized protein LOC111803706 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 260 bits (664), Expect = 3.69e-84
Identity = 149/231 (64.50%), Postives = 173/231 (74.89%), Query Frame = 0

Query: 1   MKTEEIGAMGVCGNLRILVKIFRFTEVFVGLAFFFWIFNRLPLAVRVSSDYISQISGFFS 60
           MKTEE+ AMG+  +LR L+K  RFTEV   +AF  W F+RLPL +RVSSDYISQISG+F+
Sbjct: 1   MKTEEVEAMGLSDHLRTLLKFLRFTEVSAAMAFLVWTFSRLPLILRVSSDYISQISGYFT 60

Query: 61  SSVFGFVLCNTIVVALIANPDRFSGQKLAGDSVETEIFNELVENGAGSSLSLPGNEPIGR 120
           S VFGF+LCNTI++ALIA P RFSG K  GD+VETE+ NELV++GA +SLSLPG+EP GR
Sbjct: 61  SPVFGFMLCNTIIIALIAKPHRFSGGKFTGDNVETELVNELVKDGALTSLSLPGSEPNGR 120

Query: 121 LCSDPEKEEIVYEDKEVISEVSS--AAALIENMDGSSPG---LGCLMPENVGEKLRRLAV 180
           LCSD E EEI YEDKEVISEV S  AAA  E ++  S G   L     E  G    R  V
Sbjct: 121 LCSDLELEEIAYEDKEVISEVRSTTAAAFTEEIEDFSSGSEKLKLAWSEFGGGVFGRAEV 180

Query: 181 EEKEAIWGGGISVESVWYGEELSNKEFQERIEAFIARELRFRREESGAVVL 226
           E  EAIWGGG+  ESV  GEELSNKEF+ RI+AFIAREL+FR EESGAVVL
Sbjct: 181 EA-EAIWGGGVLPESVRNGEELSNKEFERRIDAFIARELKFRWEESGAVVL 230

BLAST of MC01g1126 vs. ExPASy TrEMBL
Match: A0A6J1IPH9 (uncharacterized protein LOC111477644 OS=Cucurbita maxima OX=3661 GN=LOC111477644 PE=4 SV=1)

HSP 1 Score: 273 bits (697), Expect = 1.74e-89
Identity = 153/231 (66.23%), Postives = 178/231 (77.06%), Query Frame = 0

Query: 1   MKTEEIGAMGVCGNLRILVKIFRFTEVFVGLAFFFWIFNRLPLAVRVSSDYISQISGFFS 60
           MKTEE+ AMG+  +LR L+K FRFTEVF  +A   W F+RLPL +RVSSDYISQISG+F+
Sbjct: 1   MKTEEVEAMGLSDHLRTLLKFFRFTEVFAAMALLVWTFSRLPLILRVSSDYISQISGYFT 60

Query: 61  SSVFGFVLCNTIVVALIANPDRFSGQKLAGDSVETEIFNELVENGAGSSLSLPGNEPIGR 120
           S VFGF+LCNTI++ALIA P RFSG+K  GD+VETE+ NELV++GA +SLSLPG+EP GR
Sbjct: 61  SPVFGFMLCNTIIIALIAKPHRFSGEKFTGDNVETELVNELVKDGALTSLSLPGSEPNGR 120

Query: 121 LCSDPEKEEIVYEDKEVISEVSS--AAALIENMDGSSPG---LGCLMPENVGEKLRRLAV 180
           LCSD E EEI YEDKEVISEVSS  AAA  E ++  S G   L     E  G +  R  V
Sbjct: 121 LCSDVELEEIAYEDKEVISEVSSTTAAAFTEEIEDFSSGSEKLKLAWSEFGGGEFERAEV 180

Query: 181 EEKEAIWGGGISVESVWYGEELSNKEFQERIEAFIARELRFRREESGAVVL 226
           EE EAIWGGG+  ESV  GEELSNKEF+ RI+AFIARELRFR EESGAVVL
Sbjct: 181 EE-EAIWGGGVLPESVRQGEELSNKEFERRIDAFIARELRFRWEESGAVVL 230

BLAST of MC01g1126 vs. ExPASy TrEMBL
Match: A0A6J1GFJ2 (uncharacterized protein LOC111453461 OS=Cucurbita moschata OX=3662 GN=LOC111453461 PE=4 SV=1)

HSP 1 Score: 264 bits (674), Expect = 5.41e-86
Identity = 149/231 (64.50%), Postives = 172/231 (74.46%), Query Frame = 0

Query: 1   MKTEEIGAMGVCGNLRILVKIFRFTEVFVGLAFFFWIFNRLPLAVRVSSDYISQISGFFS 60
           MKTEE+ AMG+  +LR L+K  RFTEVF  +AF  W F+RLPL +RVSSDYISQISG+F+
Sbjct: 1   MKTEEVEAMGLSDHLRTLLKFLRFTEVFAAMAFLVWTFSRLPLILRVSSDYISQISGYFT 60

Query: 61  SSVFGFVLCNTIVVALIANPDRFSGQKLAGDSVETEIFNELVENGAGSSLSLPGNEPIGR 120
           S VFGF+LCNTI++ALIA P RFSG K  GD+VETE+ NELV++GA +SLSLPG+ P GR
Sbjct: 61  SPVFGFMLCNTIIIALIAKPPRFSGGKFTGDNVETELVNELVKDGALTSLSLPGSGPNGR 120

Query: 121 LCSDPEKEEIVYEDKEVISEVSSAAAL-----IENMDGSSPGLGCLMPENVGEKLRRLAV 180
           LCSD E EEI YEDKEVISEVSS  A      IE+    S  L     E  G +  R  V
Sbjct: 121 LCSDLELEEIAYEDKEVISEVSSTTAAAFTEEIEDFCSGSEKLKLAWSEFGGGEFGRAEV 180

Query: 181 EEKEAIWGGGISVESVWYGEELSNKEFQERIEAFIARELRFRREESGAVVL 226
           E  EAIWGGG+  ESV  GEELSNKEF+ RI+AFIARELRFR EESGAVVL
Sbjct: 181 EA-EAIWGGGVLPESVRQGEELSNKEFERRIDAFIARELRFRWEESGAVVL 230

BLAST of MC01g1126 vs. ExPASy TrEMBL
Match: A0A5A7SYQ4 (Putative TRNA--methyltransferase non-catalytic subunit trm6MTase subunit trm6 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G001910 PE=4 SV=1)

HSP 1 Score: 254 bits (649), Expect = 2.87e-82
Identity = 146/233 (62.66%), Postives = 173/233 (74.25%), Query Frame = 0

Query: 1   MKTEEIGAMGVCGNLRILVKIFRFTEVFVGLAFFFWIFNRLPLAVRVSSDYISQISGFFS 60
           MKTE + AMGV   L+ +VKIFRFTEV   + F  W F+RLPL ++VSSD+ISQIS +F+
Sbjct: 1   MKTEGVKAMGVYNLLQSMVKIFRFTEVLGAMGFLIWTFSRLPLVLKVSSDFISQISNYFT 60

Query: 61  SSVFGFVLCNTIVVALIANPDRFSGQKLAGDS-VETEIFNELVENGAGSSLSLPGNEPIG 120
           S VFGF+LCN I+V+LIA P  FSG K+ GD+ VETE+FNELVE+GA +SLSL G+EP G
Sbjct: 61  SPVFGFILCNIIIVSLIAKPHNFSGGKINGDNDVETELFNELVEDGAVTSLSLAGSEPNG 120

Query: 121 RLCSDPEKEEIVYEDKEVISEVSSAAALI-----ENMDGSSPGLGCLMPENVGEKLRRLA 180
           RLCSD E+E+I YEDKEVISEV+S  A +     EN    S  L  +  E  G +LRRL 
Sbjct: 121 RLCSDTEQEKIAYEDKEVISEVTSTRAAVFTEKMENFGLESEKLKLVWTEFGGGELRRLK 180

Query: 181 VEEKEAIWGGGISVESVWYGEELSNKEFQERIEAFIARELRFRREESGAVVLR 227
            EE EAIWGGG+S E V   EELSN EFQ RI+AFIARELRFR EESGAVVLR
Sbjct: 181 AEE-EAIWGGGVSPERVGNREELSNMEFQRRIDAFIARELRFRWEESGAVVLR 232

BLAST of MC01g1126 vs. ExPASy TrEMBL
Match: A0A1S3B3G2 (uncharacterized protein LOC103485351 OS=Cucumis melo OX=3656 GN=LOC103485351 PE=4 SV=1)

HSP 1 Score: 254 bits (649), Expect = 2.87e-82
Identity = 146/233 (62.66%), Postives = 173/233 (74.25%), Query Frame = 0

Query: 1   MKTEEIGAMGVCGNLRILVKIFRFTEVFVGLAFFFWIFNRLPLAVRVSSDYISQISGFFS 60
           MKTE + AMGV   L+ +VKIFRFTEV   + F  W F+RLPL ++VSSD+ISQIS +F+
Sbjct: 1   MKTEGVKAMGVYNLLQSMVKIFRFTEVLGAMGFLIWTFSRLPLVLKVSSDFISQISNYFT 60

Query: 61  SSVFGFVLCNTIVVALIANPDRFSGQKLAGDS-VETEIFNELVENGAGSSLSLPGNEPIG 120
           S VFGF+LCN I+V+LIA P  FSG K+ GD+ VETE+FNELVE+GA +SLSL G+EP G
Sbjct: 61  SPVFGFILCNIIIVSLIAKPHNFSGGKINGDNDVETELFNELVEDGAVTSLSLAGSEPNG 120

Query: 121 RLCSDPEKEEIVYEDKEVISEVSSAAALI-----ENMDGSSPGLGCLMPENVGEKLRRLA 180
           RLCSD E+E+I YEDKEVISEV+S  A +     EN    S  L  +  E  G +LRRL 
Sbjct: 121 RLCSDTEQEKIAYEDKEVISEVTSTRAAVFTEKMENFGLESEKLKLVWTEFGGGELRRLK 180

Query: 181 VEEKEAIWGGGISVESVWYGEELSNKEFQERIEAFIARELRFRREESGAVVLR 227
            EE EAIWGGG+S E V   EELSN EFQ RI+AFIARELRFR EESGAVVLR
Sbjct: 181 AEE-EAIWGGGVSPERVGNREELSNMEFQRRIDAFIARELRFRWEESGAVVLR 232

BLAST of MC01g1126 vs. ExPASy TrEMBL
Match: A0A0A0KM84 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G502870 PE=4 SV=1)

HSP 1 Score: 253 bits (647), Expect = 5.77e-82
Identity = 148/233 (63.52%), Postives = 174/233 (74.68%), Query Frame = 0

Query: 1   MKTEEIGAMGVCGNLRILVKIFRFTEVFVGLAFFFWIFNRLPLAVRVSSDYISQISGFFS 60
           MKT E+ AMGV   L+I+VKIFRFTEV V + F  W F+RLPL +RVSSD+ISQIS +F+
Sbjct: 1   MKTVEVKAMGVYKLLQIMVKIFRFTEVLVAMGFLIWTFSRLPLVLRVSSDFISQISNYFT 60

Query: 61  SSVFGFVLCNTIVVALIANPDRFSGQKLAGD-SVETEIFNELVENGAGSSLSLPGNEPIG 120
           S VFGF+LCN I+V+LIA P  FS  K+  D +VET +FNELVE+GA +SLSL G+EP G
Sbjct: 61  SPVFGFILCNIIIVSLIAKPHNFSCGKINSDKNVETVLFNELVEDGAVTSLSLAGSEPNG 120

Query: 121 RLCSDPEKEEIVYEDKEVISEVSSA--AALIENMDG---SSPGLGCLMPENVGEKLRRLA 180
           RLCSD E+EEI YEDKEVISEV+S   A   E M+     S  L  L  E  G +LRR+ 
Sbjct: 121 RLCSDTEQEEIAYEDKEVISEVTSTQTAVFSEKMEDFGLESEKLKLLWREYGGGELRRVK 180

Query: 181 VEEKEAIWGGGISVESVWYGEELSNKEFQERIEAFIARELRFRREESGAVVLR 227
            EE EAIWGGG+S E V  GEELSNKEF+ RI+AFIARELRFR EESGAVVLR
Sbjct: 181 AEE-EAIWGGGVSREGVGNGEELSNKEFERRIDAFIARELRFRWEESGAVVLR 232

BLAST of MC01g1126 vs. TAIR 10
Match: AT5G66440.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT4G34560.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 84.0 bits (206), Expect = 1.7e-16
Identity = 78/252 (30.95%), Postives = 122/252 (48.41%), Query Frame = 0

Query: 1   MKTEEIGAMGVCGNLRILVKIFRFTEVFVGLAFFFWIFNRLPLAVRVSSDYISQISGFFS 60
           +K E+  A+        + + FR  EV V L F FW F++LP  V++S  ++ +I+   S
Sbjct: 9   VKAEKAKALRRFNRFCRIGRFFRAAEVCVALLFIFWTFSQLPFVVQISGAFLRRIAAVIS 68

Query: 61  SSVFGFVLCNTIVVALIANPDRFSGQKLAGDSVETEIFNELVENGAGSSLSLPGNEPIGR 120
           + +F F+L N+IVV L+      +   +A  S ETEI+   V   +  + S P +E +  
Sbjct: 69  TPLFVFLLGNSIVVVLLTKSSDQTTTAVA--SAETEIYQAFVR--SVENRSKPSDEDL-- 128

Query: 121 LCSDPEKEEIVYEDKEVI-SEVSSAAALI--ENM---------------------DGSSP 180
                  E IVY+DK+VI +++ S   L   ENM                     D S  
Sbjct: 129 ------TEGIVYDDKQVIVTDLDSNPNLTVDENMPHVEIDSDSSPHSKVYGRSKSDVSGK 188

Query: 181 GLGCLMPENVGEKLRRLAVEE--KEAIWGGGISVESVWYGEELSNKEFQERIEAFIAREL 227
               ++   +   L+R   E+  KE         E  +  ++LSN+EFQ+ IEAFIA++ 
Sbjct: 189 QSSNMVMMMMKRSLQRSETEKFCKETENDDDSEEEKNYPEDDLSNEEFQKTIEAFIAKQR 248

BLAST of MC01g1126 vs. TAIR 10
Match: AT4G34560.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G66440.1); Has 67 Blast hits to 66 proteins in 11 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 67; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 44.7 bits (104), Expect = 1.2e-04
Identity = 48/216 (22.22%), Postives = 93/216 (43.06%), Query Frame = 0

Query: 15  LRILVKIFRFTEVFVGLAFFFWIFNRLPL-AVRVSSDYISQISGFFSSSVFGFVLCNTIV 74
           +R    +FR  E    L     + ++L   +V++S D   + + F  S  F F + N IV
Sbjct: 23  IRSFTSLFRLFE----LLLLVILISKLSFPSVKISGDIFKEAAVFLVSPRFVFFIGNAIV 82

Query: 75  VALIANPDRFSG----QKLAGDSVETEIFNELVENGAGSSLSLPGNEPIGRLCSDPEKEE 134
           + L+A   R+S      K A ++   +++ E +         +   +        P   +
Sbjct: 83  ITLLAKSGRYSSSHEPSKTATEAASNDLYQEFLHKSEKKKPVVYATKT--EQLKKPSGGK 142

Query: 135 IVYEDKEVISEVSSAAALIENMDGSSPGLGCLMPENVGEKLRRLAVEEKEAIWGGGISVE 194
            V   +    +   A   +E+ +G        M   V EK  R+   +K+ +    + V+
Sbjct: 143 RVSFGRSQSHKAFEAVKPLESTNGEKN-----MKRYVSEKHMRVCDSDKKVV----VRVK 202

Query: 195 SVWYGEELSNKEFQERIEAFIARELRFRREESGAVV 226
               G  +SN++F+ +IEAFIAR+ R +++E   ++
Sbjct: 203 KPEDG--MSNEQFRTKIEAFIARQKRIQKDEEHLII 221