MC01g0751 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC01g0751
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	(1->3)-beta-glucan endohydrolase
Location	MC01: 13672640 .. 13674082 (+)
RNA-Seq Expression	MC01g0751
Synteny	MC01g0751

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATCGGCGTGAACTGGGGAACAATTGCATCGCACCCATTGGAGGCAAGCGTGGTGGTCAAAATGCTGAAGGATAACGGAATAAAGAAAGTGAAGCTCTTCGATTTGGACTCGGTGGCTCATCTATCAGCCTTGTCTGGCACCGGCATCGAGACCATCGTCGGCGTCCCTAATTATCGCCTTCAGAAATTTGCAACTAACTACGACTACGTCAAAGATTGGGTTAAACAAAATATTTCTGCTTACTTGCATGAAGGGGGTGTTGATATCAAATACATCGCCGTCGGAAACGAGCCATTCTTGGCGGCTTACAAAGGAGATTACGTCAACATCACATTCCCGGCCATGCAAAACGTGCAGAAAGCCTTGGATGCGGCTGGCCATGGCGACAAAGTCAAAGTCACTACACCGCTAAACGGCGACGTTTACCAATCCTTCTCCAACCTACCTTCCGACGGCAAGTTCCGCGCCGACATTTACGATTCCATGAAGCAAATAGTTAAATTTCTCGACGAACACAAATCGCCTTTCATGGTCAACATCTACCCTTTCATCAATCTCTATCAAAATCCCGGCTTTCCGTTCAATTACGCCTTCCTTGACAACGACGGCAAGGTCAAAGACAGCGTCGTCGACGACAAGGGCACCATTTACTACAACGTCTTGGACGCACATTACGACACGCTGACGTGGTCGCTGAAGGACATGGGCGTAAACGACATGAAAATCATCATCGGAGAAGTCGGGTGGCCAACGGACGGGAATATATACGCGAACGTGACGTTGGCGAAGAGATTCTACAACGGGCTGTTCAAGAGAGTGGCGTCCAACAAAGGGACACCAATGAGACCGAATGAGAAATACGAAATTTACCTATTTAGTTTTCTGGACGAGGACGTCAAAAGTGTTGACCCGGGGAATTTTGAGCGGCATTGGGGGATTTTCCGATATGACGGGACCCCCAAGTTCGCGATGGACTTTTCCGGAGAGGGTGTGGAGAAGATGCCGGTGGCGGCGAAGGGGGTGGAGTATATGGAGCGTAAGTGGTGCGTTGTTGCAGATAATGTGACTGATTTGGGTGGAGATATACAAAAAGATGTGTCGTGGGCGTGCGAGCATTCGGATTGCACGAGCTTGGGGTATGGGTGTTCATGCAACAAATTGGATGCGCGAGGGAACGTTTCGTATGTTTTTAATATGTATTATCAAATGCAGAGCCAGAACCCGGAGGCGTGTGGTTTCGGAGGTGCAGCGGAGATTGTGACGACGAATACAACGGTTGGGGACTGCTTGTTTCCGATAATGATTAAGAGCGAGGGCGACGCCAGTGCGGGCCCAAGCGGGAGCCCCGGCGCGAGCCCGAGCGACACGAGCAAGAGTGCTAGCGAGAGGTTGAGGATCGCTGTAGCGGCAGCGCTGGTTGGGCTCGTGCTGTCGAGCCTACTT

mRNA sequence

ATCGGCGTGAACTGGGGAACAATTGCATCGCACCCATTGGAGGCAAGCGTGGTGGTCAAAATGCTGAAGGATAACGGAATAAAGAAAGTGAAGCTCTTCGATTTGGACTCGGTGGCTCATCTATCAGCCTTGTCTGGCACCGGCATCGAGACCATCGTCGGCGTCCCTAATTATCGCCTTCAGAAATTTGCAACTAACTACGACTACGTCAAAGATTGGGTTAAACAAAATATTTCTGCTTACTTGCATGAAGGGGGTGTTGATATCAAATACATCGCCGTCGGAAACGAGCCATTCTTGGCGGCTTACAAAGGAGATTACGTCAACATCACATTCCCGGCCATGCAAAACGTGCAGAAAGCCTTGGATGCGGCTGGCCATGGCGACAAAGTCAAAGTCACTACACCGCTAAACGGCGACGTTTACCAATCCTTCTCCAACCTACCTTCCGACGGCAAGTTCCGCGCCGACATTTACGATTCCATGAAGCAAATAGTTAAATTTCTCGACGAACACAAATCGCCTTTCATGGTCAACATCTACCCTTTCATCAATCTCTATCAAAATCCCGGCTTTCCGTTCAATTACGCCTTCCTTGACAACGACGGCAAGGTCAAAGACAGCGTCGTCGACGACAAGGGCACCATTTACTACAACGTCTTGGACGCACATTACGACACGCTGACGTGGTCGCTGAAGGACATGGGCGTAAACGACATGAAAATCATCATCGGAGAAGTCGGGTGGCCAACGGACGGGAATATATACGCGAACGTGACGTTGGCGAAGAGATTCTACAACGGGCTGTTCAAGAGAGTGGCGTCCAACAAAGGGACACCAATGAGACCGAATGAGAAATACGAAATTTACCTATTTAGTTTTCTGGACGAGGACGTCAAAAGTGTTGACCCGGGGAATTTTGAGCGGCATTGGGGGATTTTCCGATATGACGGGACCCCCAAGTTCGCGATGGACTTTTCCGGAGAGGGTGTGGAGAAGATGCCGGTGGCGGCGAAGGGGGTGGAGTATATGGAGCGTAAGTGGTGCGTTGTTGCAGATAATGTGACTGATTTGGGTGGAGATATACAAAAAGATGTGTCGTGGGCGTGCGAGCATTCGGATTGCACGAGCTTGGGGTATGGGTGTTCATGCAACAAATTGGATGCGCGAGGGAACGTTTCGTATGTTTTTAATATGTATTATCAAATGCAGAGCCAGAACCCGGAGGCGTGTGGTTTCGGAGGTGCAGCGGAGATTGTGACGACGAATACAACGGTTGGGGACTGCTTGTTTCCGATAATGATTAAGAGCGAGGGCGACGCCAGTGCGGGCCCAAGCGGGAGCCCCGGCGCGAGCCCGAGCGACACGAGCAAGAGTGCTAGCGAGAGGTTGAGGATCGCTGTAGCGGCAGCGCTGGTTGGGCTCGTGCTGTCGAGCCTACTT

Coding sequence (CDS)

ATCGGCGTGAACTGGGGAACAATTGCATCGCACCCATTGGAGGCAAGCGTGGTGGTCAAAATGCTGAAGGATAACGGAATAAAGAAAGTGAAGCTCTTCGATTTGGACTCGGTGGCTCATCTATCAGCCTTGTCTGGCACCGGCATCGAGACCATCGTCGGCGTCCCTAATTATCGCCTTCAGAAATTTGCAACTAACTACGACTACGTCAAAGATTGGGTTAAACAAAATATTTCTGCTTACTTGCATGAAGGGGGTGTTGATATCAAATACATCGCCGTCGGAAACGAGCCATTCTTGGCGGCTTACAAAGGAGATTACGTCAACATCACATTCCCGGCCATGCAAAACGTGCAGAAAGCCTTGGATGCGGCTGGCCATGGCGACAAAGTCAAAGTCACTACACCGCTAAACGGCGACGTTTACCAATCCTTCTCCAACCTACCTTCCGACGGCAAGTTCCGCGCCGACATTTACGATTCCATGAAGCAAATAGTTAAATTTCTCGACGAACACAAATCGCCTTTCATGGTCAACATCTACCCTTTCATCAATCTCTATCAAAATCCCGGCTTTCCGTTCAATTACGCCTTCCTTGACAACGACGGCAAGGTCAAAGACAGCGTCGTCGACGACAAGGGCACCATTTACTACAACGTCTTGGACGCACATTACGACACGCTGACGTGGTCGCTGAAGGACATGGGCGTAAACGACATGAAAATCATCATCGGAGAAGTCGGGTGGCCAACGGACGGGAATATATACGCGAACGTGACGTTGGCGAAGAGATTCTACAACGGGCTGTTCAAGAGAGTGGCGTCCAACAAAGGGACACCAATGAGACCGAATGAGAAATACGAAATTTACCTATTTAGTTTTCTGGACGAGGACGTCAAAAGTGTTGACCCGGGGAATTTTGAGCGGCATTGGGGGATTTTCCGATATGACGGGACCCCCAAGTTCGCGATGGACTTTTCCGGAGAGGGTGTGGAGAAGATGCCGGTGGCGGCGAAGGGGGTGGAGTATATGGAGCGTAAGTGGTGCGTTGTTGCAGATAATGTGACTGATTTGGGTGGAGATATACAAAAAGATGTGTCGTGGGCGTGCGAGCATTCGGATTGCACGAGCTTGGGGTATGGGTGTTCATGCAACAAATTGGATGCGCGAGGGAACGTTTCGTATGTTTTTAATATGTATTATCAAATGCAGAGCCAGAACCCGGAGGCGTGTGGTTTCGGAGGTGCAGCGGAGATTGTGACGACGAATACAACGGTTGGGGACTGCTTGTTTCCGATAATGATTAAGAGCGAGGGCGACGCCAGTGCGGGCCCAAGCGGGAGCCCCGGCGCGAGCCCGAGCGACACGAGCAAGAGTGCTAGCGAGAGGTTGAGGATCGCTGTAGCGGCAGCGCTGGTTGGGCTCGTGCTGTCGAGCCTACTT

Protein sequence

IGVNWGTIASHPLEASVVVKMLKDNGIKKVKLFDLDSVAHLSALSGTGIETIVGVPNYRLQKFATNYDYVKDWVKQNISAYLHEGGVDIKYIAVGNEPFLAAYKGDYVNITFPAMQNVQKALDAAGHGDKVKVTTPLNGDVYQSFSNLPSDGKFRADIYDSMKQIVKFLDEHKSPFMVNIYPFINLYQNPGFPFNYAFLDNDGKVKDSVVDDKGTIYYNVLDAHYDTLTWSLKDMGVNDMKIIIGEVGWPTDGNIYANVTLAKRFYNGLFKRVASNKGTPMRPNEKYEIYLFSFLDEDVKSVDPGNFERHWGIFRYDGTPKFAMDFSGEGVEKMPVAAKGVEYMERKWCVVADNVTDLGGDIQKDVSWACEHSDCTSLGYGCSCNKLDARGNVSYVFNMYYQMQSQNPEACGFGGAAEIVTTNTTVGDCLFPIMIKSEGDASAGPSGSPGASPSDTSKSASERLRIAVAAALVGLVLSSLL

Homology

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BLAST of MC01g0751 vs. ExPASy Swiss-Prot
Match: Q6NKW9 (Glucan endo-1,3-beta-glucosidase 8 OS=Arabidopsis thaliana OX=3702 GN=At1g64760 PE=2 SV=2)

HSP 1 Score: 479.2 bits (1232), Expect = 5.6e-134
Identity = 229/444 (51.58%), Postives = 303/444 (68.24%), Query Frame = 0


Query: 1   IGVNWGTIASHPLEASVVVKMLKDNGIKKVKLFDLDSVAHLSALSGTGIETIVGVPNYRL 60
           +GVNWGT+A+H L    VV+MLKDN I KVKLFD D    + AL+G+G+E +V +PN +L
Sbjct: 25  LGVNWGTMATHKLPPKTVVQMLKDNNINKVKLFDADETT-MGALAGSGLEVMVAIPNDQL 84

Query: 61  QKFATNYDYVKDWVKQNISAYLHEGGVDIKYIAVGNEPFLAAYKGDYVNITFPAMQNVQK 120
            K  T+YD  KDWV++N++ Y  +GGV+I ++AVGNEPFL +Y G ++N+TFPA+ N+Q 
Sbjct: 85  -KVMTSYDRAKDWVRKNVTRYNFDGGVNITFVAVGNEPFLKSYNGSFINLTFPALANIQN 144

Query: 121 ALDAAGHGDKVKVTTPLNGDVYQSFSN--LPSDGKFRADIYDSMKQIVKFLDEHKSPFMV 180
           AL+ AG G+ VK T PLN DVY S ++  +PS G+FR DI   M QIV FL ++ +P  +
Sbjct: 145 ALNEAGLGNSVKATVPLNADVYDSPASNPVPSAGRFRPDIIGQMTQIVDFLGKNNAPITI 204

Query: 181 NIYPFINLYQNPGFPFNYAFLDNDGKVKDSVVDDKGTIYYNVLDAHYDTLTWSLKDMGVN 240
           NIYPF++LY N  FP NYAF D    + D+ +D     Y NV DA++DTL  SLK +G  
Sbjct: 205 NIYPFLSLYGNDDFPLNYAFFDGAEPINDNGID-----YTNVFDANFDTLVSSLKAVGHG 264

Query: 241 DMKIIIGEVGWPTDGNIYANVTLAKRFYNGLFKRVASNKGTPMRPNEKYEIYLFSFLDED 300
           DM II+GEVGWPT+G+ +AN   A RFYNGL  R+ +NKGTP+RP    E+YLF  LDED
Sbjct: 265 DMPIIVGEVGWPTEGDKHANAGSAYRFYNGLLPRLGTNKGTPLRPT-YIEVYLFGLLDED 324

Query: 301 VKSVDPGNFERHWGIFRYDGTPKFAMDFSGEGVEKMPVAAKGVEYMERKWCVVADNVTDL 360
            KS+ PG FERHWGIF++DG PKF +D SG+G  K  + A+ V Y+  KWC       DL
Sbjct: 325 AKSIAPGPFERHWGIFKFDGQPKFPIDLSGQGQSKFLIGAQNVPYLPNKWCTFNPEAKDL 384

Query: 361 GGDIQKDVSWACEHSDCTSLGYGCSCNKLDARGNVSYVFNMYYQMQSQNPEACGFGGAAE 420
              +  ++ +AC  SDCT+LGYG SCN LDA GN SY FNM++Q+++Q+  AC F G A 
Sbjct: 385 -TKLAANIDYACTFSDCTALGYGSSCNTLDANGNASYAFNMFFQVKNQDESACYFQGLAT 444

Query: 421 IVTTNTTVGDCLFPIMIKSEGDAS 443
           I T N + G C FPI I +   +S
Sbjct: 445 ITTQNISQGQCNFPIQIVASSASS 459

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BLAST of MC01g0751 vs. ExPASy Swiss-Prot
Match: Q9M088 (Glucan endo-1,3-beta-glucosidase 5 OS=Arabidopsis thaliana OX=3702 GN=At4g31140 PE=2 SV=1)

HSP 1 Score: 476.1 bits (1224), Expect = 4.7e-133
Identity = 236/450 (52.44%), Postives = 304/450 (67.56%), Query Frame = 0


Query: 1   IGVNWGTIASHPLEASVVVKMLKDNGIKKVKLFDLDSVAHLSALSGTGIETIVGVPNYRL 60
           IGVNWG+ A HPL  + VV++L++NGI+KVKLF+ DS A L ALS TGI+ +VG+PN  L
Sbjct: 27  IGVNWGSQARHPLPPATVVRLLRENGIQKVKLFEADS-AILKALSRTGIQVMVGIPNDLL 86

Query: 61  QKFATNYDYVKDWVKQNISAYLHEGGVDIKYIAVGNEPFLAAYKGDYVNITFPAMQNVQK 120
              A +    + WV QN+SA++   GVDI+Y+AVGNEPFL A+ G +  IT PA+QN+Q 
Sbjct: 87  APLAGSVAAAERWVSQNVSAHVSSNGVDIRYVAVGNEPFLKAFNGTFEGITLPALQNIQS 146

Query: 121 ALDAAGHGDKVKVTTPLNGDVYQSFSNLPSDGKFRADIYDSMKQIVKFLDEHKSPFMVNI 180
           A+  AG   +VKVT PLN DVYQS SNLPSDG FR +I D M  IVKFL ++ +PF +NI
Sbjct: 147 AIIKAGLATQVKVTVPLNADVYQSASNLPSDGDFRPEIRDLMLNIVKFLSDNGAPFTINI 206

Query: 181 YPFINLYQNPGFPFNYAFLDNDGKVKDSVVDDKGTIYYNVLDAHYDTLTWSLKDMGVNDM 240
           YPFI+LY +P FP  +AF D  G    + ++D G IY NVLDA+YDTL WSL+  G  ++
Sbjct: 207 YPFISLYNDPNFPVEFAFFDGTG----TPINDNGRIYDNVLDANYDTLVWSLQKNGFGNL 266

Query: 241 KIIIGEVGWPTDGNIYANVTLAKRFYNGLFKRVASNKGTPMRPNEKYEIYLFSFLDEDVK 300
            II+GEVGWPTDG+  AN+  A+R+  G   R  +NKGTPMRP    + YLF  +DED K
Sbjct: 267 TIIVGEVGWPTDGDKNANLMYARRYNQGFMNRQKANKGTPMRPG-AMDAYLFGLIDEDAK 326

Query: 301 SVDPGNFERHWGIFRYDGTPKFAMDF-SGEGVEKMPVAAKGVEYMERKWCVVADNVTDLG 360
           S+ PGNFERHWGIF  DG PK+ +   SG G+    + AK V Y+ +KWC++A N     
Sbjct: 327 SIQPGNFERHWGIFYIDGQPKYQLSLGSGNGL----IPAKDVHYLAKKWCILAPNANLQD 386

Query: 361 GDIQKDVSWACEHSDCTSLGYGCSCNKLDARGNVSYVFNMYYQMQSQNPEACGFGGAAEI 420
             +   VS+AC+H+DCTSLGYG SC  L+   NVSY FN YYQ+ +Q   AC F G + +
Sbjct: 387 PQLGPSVSYACDHADCTSLGYGSSCGNLNLAQNVSYAFNSYYQVSNQLDSACKFPGLSIV 446

Query: 421 VTTNTTVGDCLFPIMIKSEGDASAGPSGSP 450
            T + +VG C F IMIKSE DAS   +  P
Sbjct: 447 STRDPSVGSCKFKIMIKSE-DASEASAMMP 465

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BLAST of MC01g0751 vs. ExPASy Swiss-Prot
Match: Q93Z08 (Glucan endo-1,3-beta-glucosidase 6 OS=Arabidopsis thaliana OX=3702 GN=At5g58090 PE=2 SV=2)

HSP 1 Score: 454.1 bits (1167), Expect = 1.9e-126
Identity = 220/436 (50.46%), Postives = 289/436 (66.28%), Query Frame = 0


Query: 1   IGVNWGTIASHPLEASVVVKMLKDNGIKKVKLFDLDSVAHLSALSGTGIETIVGVPNYRL 60
           IG NWGT ASHPL   +VV+ML++NGI+KVKLFD +    L AL  +GIE +VG+PN  L
Sbjct: 22  IGANWGTQASHPLPPDIVVRMLRENGIQKVKLFDAE-YDTLRALGKSGIEVMVGIPNEML 81

Query: 61  QKFATNYDYVKDWVKQNISAYLHEGGVDIKYIAVGNEPFLAAYKGDYVNITFPAMQNVQK 120
              A++    + WV +N+S ++    V+I+Y+AVGNEPFL+ Y G Y++ TFPA++N+Q 
Sbjct: 82  ATLASSLKAAEKWVAKNVSTHISTDNVNIRYVAVGNEPFLSTYNGSYLSTTFPALRNIQI 141

Query: 121 ALDAAGHGDKVKVTTPLNGDVYQSFSNLPSDGKFRADIYDSMKQIVKFLDEHKSPFMVNI 180
           A+  AG  ++VKVT PLN DVY S +  PS G FRA+I D M  IVKFL E+  PF VNI
Sbjct: 142 AIIKAGLQNQVKVTCPLNADVYDSSTTFPSGGDFRANIRDLMITIVKFLSENGGPFTVNI 201

Query: 181 YPFINLYQNPGFPFNYAFLDNDGKVKDSVVDDKGTIYYNVLDAHYDTLTWSLKDMGVNDM 240
           YP+I+LY NP FP +YAF D + +     ++D GT YYN+ DA+YDTL  +L+  G  +M
Sbjct: 202 YPYISLYTNPDFPVDYAFFDGNAQ----PLNDGGTFYYNMFDANYDTLVHALEKNGFGNM 261

Query: 241 KIIIGEVGWPTDGNIYANVTLAKRFYNGLFKRVASNKGTPMRPNEKYEIYLFSFLDEDVK 300
            IIIGE+GWPTDG+  AN+  AK+F  G    ++  KGTP RP    + YLFS +DED K
Sbjct: 262 PIIIGEIGWPTDGDSNANLDYAKKFNQGFMAHISGGKGTPRRPG-PIDAYLFSLIDEDAK 321

Query: 301 SVDPGNFERHWGIFRYDGTPKFAMDFSGEGVEKMPVAAKGVEYMERKWCVVADNVTDLGG 360
           SV PG FERHWGIF +DG PK+A++        + + AKGV Y+ERKWCV+  NV     
Sbjct: 322 SVQPGYFERHWGIFTFDGLPKYALNLGTTNTGAL-IQAKGVRYLERKWCVMKPNVRLDDP 381

Query: 361 DIQKDVSWACEHSDCTSLGYGCSCNKLDARGNVSYVFNMYYQMQSQNPEACGFGGAAEIV 420
            +   VS+AC   DCTSLG G SC  LD + N+SY FN YYQ+Q Q   AC F   +E+ 
Sbjct: 382 QVAPAVSYACSLGDCTSLGVGTSCANLDGKQNISYAFNSYYQIQDQLDTACKFPNISEVT 441

Query: 421 TTNTTVGDCLFPIMIK 437
            T+ + G C FPIMI+
Sbjct: 442 KTDPSTGTCRFPIMIE 450

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BLAST of MC01g0751 vs. ExPASy Swiss-Prot
Match: Q9FGH4 (Glucan endo-1,3-beta-glucosidase 9 OS=Arabidopsis thaliana OX=3702 GN=At5g58480 PE=2 SV=1)

HSP 1 Score: 320.1 bits (819), Expect = 4.3e-86
Identity = 174/447 (38.93%), Postives = 245/447 (54.81%), Query Frame = 0


Query: 1   IGVNWGTIASHPLEASVVVKMLKDNGIKKVKLFDLDSVAHLSALSGTGIETIVGVPNYRL 60
           +G+NWGT ASHPL  S VV++LK NGI KVKLFD D    L ALSG+ I   +G+ N  L
Sbjct: 26  VGINWGTEASHPLPPSKVVELLKSNGIVKVKLFDADPKV-LRALSGSNIGVTIGIQNSML 85

Query: 61  QKFATNYDYVKDWVKQNISAYLHEGG-VDIKYIAVGNEPFLAAYKGDYVNITFPAMQNVQ 120
           +    +    + WV  N++ Y + G  V I+Y+AVG EPFL +Y   Y      A  N+Q
Sbjct: 86  KSLNASVKVAESWVHDNVTRYFNGGNRVRIEYVAVGEEPFLQSYGNQYKPFVIGAAMNIQ 145

Query: 121 KALDAAGHGDKVKVTTPLNGDVYQSFSNLPSDGKFRADIYDSMKQIVKFLDEHKSPFMVN 180
            AL  A   ++VKV  P + D + S S  PS G FRAD+  +M +++ FL +H SPF V 
Sbjct: 146 NALVKANLANEVKVVVPSSFDSFLSESGRPSSGHFRADLNKTMIELLSFLTKHHSPFFVT 205

Query: 181 IYPFINLYQNPGFPFNYAFLDNDGKVKDSVVDDKGTIYYNVLDAHYDTLTWSLKDMGVND 240
           I PF++ +QN     +++      K       D    Y N  D  YDTL  +L  +G ++
Sbjct: 206 ISPFLSFHQNKNISLDFSLFKETAKAH----KDGRKTYRNSFDLSYDTLVSALFTIGFSE 265

Query: 241 MKIIIGEVGWPTDGNIYANVTLAKRFYNGLFKRVASNKGTPMRPNEKYEIYLFSFLDEDV 300
           + I++ ++GWPTDG   A    A+ F+ GL   V   K T   P    E Y+ S LDED 
Sbjct: 266 VDIVVSKIGWPTDGAENATSLTAEAFFKGLI--VHLEKKTASLPRPPVETYIESLLDEDQ 325

Query: 301 KSVDPGNFERHWGIFRYDGTPKFAMDFSGEGVEKMPVAAKGVEYMERKWCVVADNVTDLG 360
           +++  GNFERHWG+F +DG  K+   F+     K  V A+ V+Y+  KWCVV +N  DL 
Sbjct: 326 RNLSAGNFERHWGVFTFDGQAKYNFSFN----HKNQVNAQNVQYLPPKWCVVNNN-KDLS 385

Query: 361 GDIQKDVSWACEHSDCTSLGYGCSCNKLDARGNVSYVFNMYYQMQSQNPEACGFGGAAEI 420
               + +  AC  +DCTS+  G SC+ +   GNVSY FN  YQ    + E+C FGG   I
Sbjct: 386 NASARALE-ACAVADCTSILPGGSCSGIRWPGNVSYAFNSLYQQNDHSAESCNFGGLGLI 445

Query: 421 VTTNTTVGDCLFPIMIKSEGDASAGPS 447
            T + +  +C F I + +   +S  P+
Sbjct: 446 TTVDPSEDNCRFSIQLDTSHSSSQTPN 459

[Close]

BLAST of MC01g0751 vs. ExPASy Swiss-Prot
Match: Q9M069 (Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 PE=1 SV=2)

HSP 1 Score: 216.9 bits (551), Expect = 5.2e-55
Identity = 144/460 (31.30%), Postives = 226/460 (49.13%), Query Frame = 0


Query: 1   IGVNWGTIASHPLEASVVVKMLKDNGIKKVKLFDLDSVAHLSALSGTGIETIVGVPNYRL 60
           IGVN+G +A +    S  VK+L+   I+KV+L+  D  A + AL+GTG+  ++G  N  +
Sbjct: 26  IGVNYGQVADNLPPPSETVKLLQSTSIQKVRLYGADP-AIIKALAGTGVGIVIGAANGDV 85

Query: 61  QKFATNYDYVKDWVKQNISAYLHEGGVDIKYIAVGNEPFLAAYKGDYVNITFPAMQNVQK 120
              A++ +    W+  N+  +       I  I VGNE  L +   + VN   PAMQNVQK
Sbjct: 86  PSLASDPNAATQWINSNVLPFYPAS--KIMLITVGNE-ILMSNDPNLVNQLLPAMQNVQK 145

Query: 121 ALDAAGHGDKVKVTTPLNGDVYQSFSNLPSDGKFRADIYDSMKQIVKFLDEHKSPFMVNI 180
           AL+A   G K+KV+T +N       S+ PS G F A     +K I++FL +  SPF +N 
Sbjct: 146 ALEAVSLGGKIKVST-VNSMTVLGSSDPPSSGSFAAGYQTGLKGILQFLSDTGSPFAINP 205

Query: 181 YPFINLYQNPGFPFNYAFL---DNDGKVKDSVVDDKGTIYYNVLDAHYDTLTWSLKDMGV 240
           YPF   YQ+   P   AF     N G+V        G  Y N+ DA  D +  +LK MG 
Sbjct: 206 YPFF-AYQSDPRPETLAFCLFEPNAGRVDSKT----GIKYTNMFDAQVDAVHSALKSMGF 265

Query: 241 NDMKIIIGEVGWPTDGN---IYANVTLAKRFYNGLFKRVASNKGTPMRPNEKYEIYLFSF 300
             ++I++ E GW + G+   + A+V  AK +   L   + S  GTP+ P +  + Y+F+ 
Sbjct: 266 EKVEIVVAETGWASRGDANEVGASVDNAKAYNGNLIAHLRSMVGTPLMPGKPVDTYIFAL 325

Query: 301 LDEDVKSVDPG-NFERHWGIFRYDGTPKFAMDFSGEGVEKMPVAAKGVEYMERKWCVVAD 360
            DE++K   PG + ER +G+F+ D +  + +  +         + K        WCV   
Sbjct: 326 YDENLK---PGPSSERAFGLFKTDLSMVYDVGLAKSSSSSQTPSGK---VTSSGWCVPKK 385

Query: 361 NVTDLGGDIQKDVSWACEHS-DCTSLGYGCSC-NKLDARGNVSYVFNMYYQMQSQNPEAC 420
             T+   ++Q  + WAC H  DC ++  G +C    +   + +Y  NMY+Q   + P  C
Sbjct: 386 GATN--EELQASLDWACGHGIDCGAIQPGGACFEPNNVVSHAAYAMNMYFQKSPKQPTDC 445

Query: 421 GFGGAAEIVTTNTTVGDCLFPIMIKSEGDASAGPSGSPGA 452
            F   A + + N +  +C++P      G    G  G  G+
Sbjct: 446 DFSKTATVTSQNPSYNNCVYP------GGGGGGGGGGGGS 461

[Close]

BLAST of MC01g0751 vs. NCBI nr
Match: XP_022132538.1 (glucan endo-1,3-beta-glucosidase 5-like isoform X1 [Momordica charantia])

HSP 1 Score: 981 bits (2537), Expect = 0.0
Identity = 481/481 (100.00%), Postives = 481/481 (100.00%), Query Frame = 0


Query: 1   IGVNWGTIASHPLEASVVVKMLKDNGIKKVKLFDLDSVAHLSALSGTGIETIVGVPNYRL 60
           IGVNWGTIASHPLEASVVVKMLKDNGIKKVKLFDLDSVAHLSALSGTGIETIVGVPNYRL
Sbjct: 30  IGVNWGTIASHPLEASVVVKMLKDNGIKKVKLFDLDSVAHLSALSGTGIETIVGVPNYRL 89

Query: 61  QKFATNYDYVKDWVKQNISAYLHEGGVDIKYIAVGNEPFLAAYKGDYVNITFPAMQNVQK 120
           QKFATNYDYVKDWVKQNISAYLHEGGVDIKYIAVGNEPFLAAYKGDYVNITFPAMQNVQK
Sbjct: 90  QKFATNYDYVKDWVKQNISAYLHEGGVDIKYIAVGNEPFLAAYKGDYVNITFPAMQNVQK 149

Query: 121 ALDAAGHGDKVKVTTPLNGDVYQSFSNLPSDGKFRADIYDSMKQIVKFLDEHKSPFMVNI 180
           ALDAAGHGDKVKVTTPLNGDVYQSFSNLPSDGKFRADIYDSMKQIVKFLDEHKSPFMVNI
Sbjct: 150 ALDAAGHGDKVKVTTPLNGDVYQSFSNLPSDGKFRADIYDSMKQIVKFLDEHKSPFMVNI 209

Query: 181 YPFINLYQNPGFPFNYAFLDNDGKVKDSVVDDKGTIYYNVLDAHYDTLTWSLKDMGVNDM 240
           YPFINLYQNPGFPFNYAFLDNDGKVKDSVVDDKGTIYYNVLDAHYDTLTWSLKDMGVNDM
Sbjct: 210 YPFINLYQNPGFPFNYAFLDNDGKVKDSVVDDKGTIYYNVLDAHYDTLTWSLKDMGVNDM 269

Query: 241 KIIIGEVGWPTDGNIYANVTLAKRFYNGLFKRVASNKGTPMRPNEKYEIYLFSFLDEDVK 300
           KIIIGEVGWPTDGNIYANVTLAKRFYNGLFKRVASNKGTPMRPNEKYEIYLFSFLDEDVK
Sbjct: 270 KIIIGEVGWPTDGNIYANVTLAKRFYNGLFKRVASNKGTPMRPNEKYEIYLFSFLDEDVK 329

Query: 301 SVDPGNFERHWGIFRYDGTPKFAMDFSGEGVEKMPVAAKGVEYMERKWCVVADNVTDLGG 360
           SVDPGNFERHWGIFRYDGTPKFAMDFSGEGVEKMPVAAKGVEYMERKWCVVADNVTDLGG
Sbjct: 330 SVDPGNFERHWGIFRYDGTPKFAMDFSGEGVEKMPVAAKGVEYMERKWCVVADNVTDLGG 389

Query: 361 DIQKDVSWACEHSDCTSLGYGCSCNKLDARGNVSYVFNMYYQMQSQNPEACGFGGAAEIV 420
           DIQKDVSWACEHSDCTSLGYGCSCNKLDARGNVSYVFNMYYQMQSQNPEACGFGGAAEIV
Sbjct: 390 DIQKDVSWACEHSDCTSLGYGCSCNKLDARGNVSYVFNMYYQMQSQNPEACGFGGAAEIV 449

Query: 421 TTNTTVGDCLFPIMIKSEGDASAGPSGSPGASPSDTSKSASERLRIAVAAALVGLVLSSL 480
           TTNTTVGDCLFPIMIKSEGDASAGPSGSPGASPSDTSKSASERLRIAVAAALVGLVLSSL
Sbjct: 450 TTNTTVGDCLFPIMIKSEGDASAGPSGSPGASPSDTSKSASERLRIAVAAALVGLVLSSL 509

[Close]

BLAST of MC01g0751 vs. NCBI nr
Match: XP_022133125.1 (glucan endo-1,3-beta-glucosidase 8-like, partial [Momordica charantia])

HSP 1 Score: 810 bits (2093), Expect = 2.87e-293
Identity = 386/483 (79.92%), Postives = 431/483 (89.23%), Query Frame = 0


Query: 1   IGVNWGTIASHPLEASVVVKMLKDNGIKKVKLFDLDSVAHLSALSGTGIETIVGVPNYRL 60
           +GVNWGT+A++PL+ S+VV++LKDNGIK+VKLFDLDSVA+LSAL+GTGI+TIVGVPNY L
Sbjct: 10  LGVNWGTVATNPLDGSIVVQLLKDNGIKRVKLFDLDSVANLSALAGTGIKTIVGVPNYLL 69

Query: 61  QKFATNYDYVKDWVKQNISAYLHEGGVDIKYIAVGNEPFLAAYKGDYVNITFPAMQNVQK 120
           Q FAT+Y+ VKDWVKQNISAY+ +GGVDI YIAVGNEPFL AYKG Y N TFPAMQNVQK
Sbjct: 70  QTFATDYNLVKDWVKQNISAYMGDGGVDIIYIAVGNEPFLGAYKGMYTNTTFPAMQNVQK 129

Query: 121 ALDAAGHGDKVKVTTPLNGDVYQSFSNLPSDGKFRADIYDSMKQIVKFLDEHKSPFMVNI 180
           AL+AAGH DK+KVTTPLNGDVYQSFSNLPSDG+FR DIYD MKQIV+FLD++KSPFM+NI
Sbjct: 130 ALEAAGHSDKIKVTTPLNGDVYQSFSNLPSDGRFRTDIYDPMKQIVQFLDKNKSPFMINI 189

Query: 181 YPFINLYQNPGFPFNYAFLDNDGKVKDSVVDDKGTIYYNVLDAHYDTLTWSLKDMGVNDM 240
           YPFINLYQNPGFPFNYAF DNDGKVKDSV+DDKGTIYYNVLDAHYDTLT+SLK+MGVNDM
Sbjct: 190 YPFINLYQNPGFPFNYAFFDNDGKVKDSVIDDKGTIYYNVLDAHYDTLTYSLKEMGVNDM 249

Query: 241 KIIIGEVGWPTDGNIYANVTLAKRFYNGLFKRVASNKGTPMRPNEKYEIYLFSFLDEDVK 300
           KIIIGEVGWPTDGNIYAN T+AKRFY+GL KR+ASNKGTPMRP E YE+Y+FS LDE++K
Sbjct: 250 KIIIGEVGWPTDGNIYANTTVAKRFYSGLLKRMASNKGTPMRPKENYEVYMFSLLDENIK 309

Query: 301 SVDPGNFERHWGIFRYDGTPKFAMDFSGEGVEKMPVAAKGVEYMERKWCVVADNVTDLGG 360
           S+DPGNFERHWGIFRYDGTPKFAMDFSG G +KMPV AKGVEYM+ +WCV+ADNVT+LG 
Sbjct: 310 SIDPGNFERHWGIFRYDGTPKFAMDFSGNGADKMPVGAKGVEYMKNQWCVIADNVTELGA 369

Query: 361 DIQKDVSWACEHSDCTSLGYGCSCNKLDARGNVSYVFNMYYQMQSQNPEACGFGGAAEIV 420
            IQKDVSWACEHS+CTSLGYGCSCN L  RGNVSYVFNMYYQMQ QNP ACGFGGAA+IV
Sbjct: 370 SIQKDVSWACEHSECTSLGYGCSCNNLGVRGNVSYVFNMYYQMQDQNPAACGFGGAAKIV 429

Query: 421 TTNTTVGDCLFPIMIK-SEGD--ASAGPSGSPGASPSDTSKSASERLRIAVAAALVGLVL 480
           T N TVGDCLFPI I  S G   AS+ P GSP  SP  + KSA E LR+  A ALVGLV+
Sbjct: 430 TKNATVGDCLFPIQITGSSGSTTASSSPGGSPSGSPGGSGKSAGESLRMRAAVALVGLVV 489

[Close]

BLAST of MC01g0751 vs. NCBI nr
Match: XP_022132539.1 (glucan endo-1,3-beta-glucosidase 5-like isoform X2 [Momordica charantia])

HSP 1 Score: 790 bits (2039), Expect = 3.21e-286
Identity = 379/379 (100.00%), Postives = 379/379 (100.00%), Query Frame = 0


Query: 1   IGVNWGTIASHPLEASVVVKMLKDNGIKKVKLFDLDSVAHLSALSGTGIETIVGVPNYRL 60
           IGVNWGTIASHPLEASVVVKMLKDNGIKKVKLFDLDSVAHLSALSGTGIETIVGVPNYRL
Sbjct: 30  IGVNWGTIASHPLEASVVVKMLKDNGIKKVKLFDLDSVAHLSALSGTGIETIVGVPNYRL 89

Query: 61  QKFATNYDYVKDWVKQNISAYLHEGGVDIKYIAVGNEPFLAAYKGDYVNITFPAMQNVQK 120
           QKFATNYDYVKDWVKQNISAYLHEGGVDIKYIAVGNEPFLAAYKGDYVNITFPAMQNVQK
Sbjct: 90  QKFATNYDYVKDWVKQNISAYLHEGGVDIKYIAVGNEPFLAAYKGDYVNITFPAMQNVQK 149

Query: 121 ALDAAGHGDKVKVTTPLNGDVYQSFSNLPSDGKFRADIYDSMKQIVKFLDEHKSPFMVNI 180
           ALDAAGHGDKVKVTTPLNGDVYQSFSNLPSDGKFRADIYDSMKQIVKFLDEHKSPFMVNI
Sbjct: 150 ALDAAGHGDKVKVTTPLNGDVYQSFSNLPSDGKFRADIYDSMKQIVKFLDEHKSPFMVNI 209

Query: 181 YPFINLYQNPGFPFNYAFLDNDGKVKDSVVDDKGTIYYNVLDAHYDTLTWSLKDMGVNDM 240
           YPFINLYQNPGFPFNYAFLDNDGKVKDSVVDDKGTIYYNVLDAHYDTLTWSLKDMGVNDM
Sbjct: 210 YPFINLYQNPGFPFNYAFLDNDGKVKDSVVDDKGTIYYNVLDAHYDTLTWSLKDMGVNDM 269

Query: 241 KIIIGEVGWPTDGNIYANVTLAKRFYNGLFKRVASNKGTPMRPNEKYEIYLFSFLDEDVK 300
           KIIIGEVGWPTDGNIYANVTLAKRFYNGLFKRVASNKGTPMRPNEKYEIYLFSFLDEDVK
Sbjct: 270 KIIIGEVGWPTDGNIYANVTLAKRFYNGLFKRVASNKGTPMRPNEKYEIYLFSFLDEDVK 329

Query: 301 SVDPGNFERHWGIFRYDGTPKFAMDFSGEGVEKMPVAAKGVEYMERKWCVVADNVTDLGG 360
           SVDPGNFERHWGIFRYDGTPKFAMDFSGEGVEKMPVAAKGVEYMERKWCVVADNVTDLGG
Sbjct: 330 SVDPGNFERHWGIFRYDGTPKFAMDFSGEGVEKMPVAAKGVEYMERKWCVVADNVTDLGG 389

Query: 361 DIQKDVSWACEHSDCTSLG 379
           DIQKDVSWACEHSDCTSLG
Sbjct: 390 DIQKDVSWACEHSDCTSLG 408

[Close]

BLAST of MC01g0751 vs. NCBI nr
Match: XP_038883272.1 (glucan endo-1,3-beta-glucosidase 8 [Benincasa hispida])

HSP 1 Score: 619 bits (1596), Expect = 7.30e-218
Identity = 303/478 (63.39%), Postives = 374/478 (78.24%), Query Frame = 0


Query: 1   IGVNWGTIASHPLEASVVVKMLKDNGIKKVKLFDLDSVAHLSALSGTGIETIVGVPNYRL 60
           IGVNWG +ASHPL  ++VVK+LKDNGIKKVKLFD DS   +S+LSG+ IET++G+PN +L
Sbjct: 29  IGVNWGIMASHPLHPNIVVKLLKDNGIKKVKLFDSDSWT-VSSLSGSKIETVIGIPNDQL 88

Query: 61  QKFATNYDYVKDWVKQNISAYLHEGGVDIKYIAVGNEPFLAAYKGDYVNITFPAMQNVQK 120
           +KFA +YD  KDWVK+N++ ++ EGGVDI+Y++VGNE FL AY G ++N TFPAM N+QK
Sbjct: 89  KKFAGSYDDAKDWVKENVTVHMFEGGVDIRYVSVGNEAFLTAYNGSFLNFTFPAMVNIQK 148

Query: 121 ALDAAGHGDKVKVTTPLNGDVYQSFSNLPSDGKFRADIYDSMKQIVKFLDEHKSPFMVNI 180
           ALDAAGHG KVKVTT LN DVY+S SNLPSDG+FR+DI+ +MK IV FLD +K+PFMVNI
Sbjct: 149 ALDAAGHGQKVKVTTALNADVYESRSNLPSDGQFRSDIFSAMKDIVHFLDRNKAPFMVNI 208

Query: 181 YPFINLYQNPGFPFNYAFLDNDGKVKDSVVDDKGTIYYNVLDAHYDTLTWSLKDMGVNDM 240
           YPF++LYQNP FP +YAF   DGK       DKG  Y NV DA+YDTL WSLK +G++D+
Sbjct: 209 YPFLSLYQNPNFPLDYAFF-GDGKP----TIDKGRTYTNVFDANYDTLIWSLKKVGISDL 268

Query: 241 KIIIGEVGWPTDGNIYANVTLAKRFYNGLFKRVASNKGTPMRPNEKYEIYLFSFLDEDVK 300
           KII+GEVGWPTD N +AN+TLAKRFY+GLFK++AS++GTPMRPNEK+E+YLF  LDED+K
Sbjct: 269 KIIVGEVGWPTDANKFANLTLAKRFYDGLFKKLASDEGTPMRPNEKFEVYLFGLLDEDMK 328

Query: 301 SVDPGNFERHWGIFRYDGTPKFAMDFSGEGVEKMPVAAKGVEYMERKWCVVADNVTDLGG 360
           S+ PG FERHWGIFR+DG PKF MDFSG+G +KM V AKGV+Y+ERKWC+V  ++ +L  
Sbjct: 329 SILPGFFERHWGIFRFDGKPKFPMDFSGKGNDKMLVGAKGVQYLERKWCIVKPSLKNLD- 388

Query: 361 DIQKDVSWACEHSDCTSLGYGCSCNKLDARGNVSYVFNMYYQMQSQNPEACGFGGAAEIV 420
           +I  +V +AC H+DCTSL YG SCN LD RGN+SY FNMYYQMQ Q+ EAC F G+AEIV
Sbjct: 389 EISSEVDYACAHADCTSLVYGSSCNHLDRRGNISYAFNMYYQMQDQSVEACVFSGSAEIV 448

Query: 421 TTNTTVGDCLFPIMIKSEGDASAGPSGSPGASPSDTSKSASERLRIAVAAALVGLVLS 478
           T N +VG CLFPI I S G                      ERL+ AVAAA+VG+VLS
Sbjct: 449 TKNASVGTCLFPIQIVSAG----------------------ERLK-AVAAAMVGIVLS 476

[Close]

BLAST of MC01g0751 vs. NCBI nr
Match: XP_008440124.1 (PREDICTED: glucan endo-1,3-beta-glucosidase 8 [Cucumis melo])

HSP 1 Score: 618 bits (1594), Expect = 1.58e-217
Identity = 301/477 (63.10%), Postives = 365/477 (76.52%), Query Frame = 0


Query: 1   IGVNWGTIASHPLEASVVVKMLKDNGIKKVKLFDLDSVAHLSALSGTGIETIVGVPNYRL 60
           IG NWG ++SHPL  ++VVK+LKDNGIKKVKLFD DS   +SALSG+ IET++G+PN +L
Sbjct: 29  IGANWGIMSSHPLRPNIVVKLLKDNGIKKVKLFDSDSWT-VSALSGSKIETVIGIPNDQL 88

Query: 61  QKFATNYDYVKDWVKQNISAYLHEGGVDIKYIAVGNEPFLAAYKGDYVNITFPAMQNVQK 120
           +KFA +YD  KDWVK+N++ ++ EGGVD++Y++VGNE FL+AY G YV +T+PAMQN+QK
Sbjct: 89  KKFAESYDDAKDWVKENVTTHMFEGGVDLRYVSVGNEAFLSAYNGTYVKVTYPAMQNIQK 148

Query: 121 ALDAAGHGDKVKVTTPLNGDVYQSFSNLPSDGKFRADIYDSMKQIVKFLDEHKSPFMVNI 180
           ALDAAGHG KVKVTT LN DVY+S +NLPSDG+FR DIY +MK IV FLD +K+PFMVNI
Sbjct: 149 ALDAAGHGKKVKVTTALNADVYESSTNLPSDGQFRPDIYKTMKDIVHFLDRNKAPFMVNI 208

Query: 181 YPFINLYQNPGFPFNYAFLDNDGKVKDSVVDDKGTIYYNVLDAHYDTLTWSLKDMGVNDM 240
           YPF++LYQNP FP +YAF     K      +DKG  Y NV DA+YDTL WSLK +GV DM
Sbjct: 209 YPFLSLYQNPNFPLDYAFFGGGAKA----TNDKGKSYTNVFDANYDTLIWSLKKIGVTDM 268

Query: 241 KIIIGEVGWPTDGNIYANVTLAKRFYNGLFKRVASNKGTPMRPNEKYEIYLFSFLDEDVK 300
           KII+GEVGWPTDGN +ANV LAKRFY+GLFK++AS  GTPMRP EK+E+YLF  LDED+K
Sbjct: 269 KIIVGEVGWPTDGNKFANVELAKRFYDGLFKKLASGNGTPMRPKEKFEVYLFGLLDEDMK 328

Query: 301 SVDPGNFERHWGIFRYDGTPKFAMDFSGEGVEKMPVAAKGVEYMERKWCVVADNVTDLGG 360
           SV PG FERHWGIF++DG PKF MD SG+G +KM VAAKGV+Y+E+KWCV+ +NV +   
Sbjct: 329 SVLPGFFERHWGIFQFDGKPKFPMDMSGKGNDKMLVAAKGVQYLEKKWCVLKENVKNFD- 388

Query: 361 DIQKDVSWACEHSDCTSLGYGCSCNKLDARGNVSYVFNMYYQMQSQNPEACGFGGAAEIV 420
            I K+V +AC  SDCTSLGYG SCN LD RGN+SY FNMYYQMQ Q+ EAC F G+A IV
Sbjct: 389 KISKEVDYACGLSDCTSLGYGSSCNNLDKRGNISYAFNMYYQMQDQSVEACDFSGSAHIV 448

Query: 421 TTNTTVGDCLFPIMIKSEGDASAGPSGSPGASPSDTSKSASERLRIAVAAALVGLVL 477
             N +VG CLFPI I S G                      ERL+ A AAA+VGLVL
Sbjct: 449 KKNASVGSCLFPIQIVSTG----------------------ERLKAAAAAAVVGLVL 477

[Close]

BLAST of MC01g0751 vs. ExPASy TrEMBL
Match: A0A6J1BWJ0 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111005372 PE=3 SV=1)

HSP 1 Score: 981 bits (2537), Expect = 0.0
Identity = 481/481 (100.00%), Postives = 481/481 (100.00%), Query Frame = 0


Query: 1   IGVNWGTIASHPLEASVVVKMLKDNGIKKVKLFDLDSVAHLSALSGTGIETIVGVPNYRL 60
           IGVNWGTIASHPLEASVVVKMLKDNGIKKVKLFDLDSVAHLSALSGTGIETIVGVPNYRL
Sbjct: 30  IGVNWGTIASHPLEASVVVKMLKDNGIKKVKLFDLDSVAHLSALSGTGIETIVGVPNYRL 89

Query: 61  QKFATNYDYVKDWVKQNISAYLHEGGVDIKYIAVGNEPFLAAYKGDYVNITFPAMQNVQK 120
           QKFATNYDYVKDWVKQNISAYLHEGGVDIKYIAVGNEPFLAAYKGDYVNITFPAMQNVQK
Sbjct: 90  QKFATNYDYVKDWVKQNISAYLHEGGVDIKYIAVGNEPFLAAYKGDYVNITFPAMQNVQK 149

Query: 121 ALDAAGHGDKVKVTTPLNGDVYQSFSNLPSDGKFRADIYDSMKQIVKFLDEHKSPFMVNI 180
           ALDAAGHGDKVKVTTPLNGDVYQSFSNLPSDGKFRADIYDSMKQIVKFLDEHKSPFMVNI
Sbjct: 150 ALDAAGHGDKVKVTTPLNGDVYQSFSNLPSDGKFRADIYDSMKQIVKFLDEHKSPFMVNI 209

Query: 181 YPFINLYQNPGFPFNYAFLDNDGKVKDSVVDDKGTIYYNVLDAHYDTLTWSLKDMGVNDM 240
           YPFINLYQNPGFPFNYAFLDNDGKVKDSVVDDKGTIYYNVLDAHYDTLTWSLKDMGVNDM
Sbjct: 210 YPFINLYQNPGFPFNYAFLDNDGKVKDSVVDDKGTIYYNVLDAHYDTLTWSLKDMGVNDM 269

Query: 241 KIIIGEVGWPTDGNIYANVTLAKRFYNGLFKRVASNKGTPMRPNEKYEIYLFSFLDEDVK 300
           KIIIGEVGWPTDGNIYANVTLAKRFYNGLFKRVASNKGTPMRPNEKYEIYLFSFLDEDVK
Sbjct: 270 KIIIGEVGWPTDGNIYANVTLAKRFYNGLFKRVASNKGTPMRPNEKYEIYLFSFLDEDVK 329

Query: 301 SVDPGNFERHWGIFRYDGTPKFAMDFSGEGVEKMPVAAKGVEYMERKWCVVADNVTDLGG 360
           SVDPGNFERHWGIFRYDGTPKFAMDFSGEGVEKMPVAAKGVEYMERKWCVVADNVTDLGG
Sbjct: 330 SVDPGNFERHWGIFRYDGTPKFAMDFSGEGVEKMPVAAKGVEYMERKWCVVADNVTDLGG 389

Query: 361 DIQKDVSWACEHSDCTSLGYGCSCNKLDARGNVSYVFNMYYQMQSQNPEACGFGGAAEIV 420
           DIQKDVSWACEHSDCTSLGYGCSCNKLDARGNVSYVFNMYYQMQSQNPEACGFGGAAEIV
Sbjct: 390 DIQKDVSWACEHSDCTSLGYGCSCNKLDARGNVSYVFNMYYQMQSQNPEACGFGGAAEIV 449

Query: 421 TTNTTVGDCLFPIMIKSEGDASAGPSGSPGASPSDTSKSASERLRIAVAAALVGLVLSSL 480
           TTNTTVGDCLFPIMIKSEGDASAGPSGSPGASPSDTSKSASERLRIAVAAALVGLVLSSL
Sbjct: 450 TTNTTVGDCLFPIMIKSEGDASAGPSGSPGASPSDTSKSASERLRIAVAAALVGLVLSSL 509

[Close]

BLAST of MC01g0751 vs. ExPASy TrEMBL
Match: A0A6J1BUF0 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111005798 PE=3 SV=1)

HSP 1 Score: 810 bits (2093), Expect = 1.39e-293
Identity = 386/483 (79.92%), Postives = 431/483 (89.23%), Query Frame = 0


Query: 1   IGVNWGTIASHPLEASVVVKMLKDNGIKKVKLFDLDSVAHLSALSGTGIETIVGVPNYRL 60
           +GVNWGT+A++PL+ S+VV++LKDNGIK+VKLFDLDSVA+LSAL+GTGI+TIVGVPNY L
Sbjct: 10  LGVNWGTVATNPLDGSIVVQLLKDNGIKRVKLFDLDSVANLSALAGTGIKTIVGVPNYLL 69

Query: 61  QKFATNYDYVKDWVKQNISAYLHEGGVDIKYIAVGNEPFLAAYKGDYVNITFPAMQNVQK 120
           Q FAT+Y+ VKDWVKQNISAY+ +GGVDI YIAVGNEPFL AYKG Y N TFPAMQNVQK
Sbjct: 70  QTFATDYNLVKDWVKQNISAYMGDGGVDIIYIAVGNEPFLGAYKGMYTNTTFPAMQNVQK 129

Query: 121 ALDAAGHGDKVKVTTPLNGDVYQSFSNLPSDGKFRADIYDSMKQIVKFLDEHKSPFMVNI 180
           AL+AAGH DK+KVTTPLNGDVYQSFSNLPSDG+FR DIYD MKQIV+FLD++KSPFM+NI
Sbjct: 130 ALEAAGHSDKIKVTTPLNGDVYQSFSNLPSDGRFRTDIYDPMKQIVQFLDKNKSPFMINI 189

Query: 181 YPFINLYQNPGFPFNYAFLDNDGKVKDSVVDDKGTIYYNVLDAHYDTLTWSLKDMGVNDM 240
           YPFINLYQNPGFPFNYAF DNDGKVKDSV+DDKGTIYYNVLDAHYDTLT+SLK+MGVNDM
Sbjct: 190 YPFINLYQNPGFPFNYAFFDNDGKVKDSVIDDKGTIYYNVLDAHYDTLTYSLKEMGVNDM 249

Query: 241 KIIIGEVGWPTDGNIYANVTLAKRFYNGLFKRVASNKGTPMRPNEKYEIYLFSFLDEDVK 300
           KIIIGEVGWPTDGNIYAN T+AKRFY+GL KR+ASNKGTPMRP E YE+Y+FS LDE++K
Sbjct: 250 KIIIGEVGWPTDGNIYANTTVAKRFYSGLLKRMASNKGTPMRPKENYEVYMFSLLDENIK 309

Query: 301 SVDPGNFERHWGIFRYDGTPKFAMDFSGEGVEKMPVAAKGVEYMERKWCVVADNVTDLGG 360
           S+DPGNFERHWGIFRYDGTPKFAMDFSG G +KMPV AKGVEYM+ +WCV+ADNVT+LG 
Sbjct: 310 SIDPGNFERHWGIFRYDGTPKFAMDFSGNGADKMPVGAKGVEYMKNQWCVIADNVTELGA 369

Query: 361 DIQKDVSWACEHSDCTSLGYGCSCNKLDARGNVSYVFNMYYQMQSQNPEACGFGGAAEIV 420
            IQKDVSWACEHS+CTSLGYGCSCN L  RGNVSYVFNMYYQMQ QNP ACGFGGAA+IV
Sbjct: 370 SIQKDVSWACEHSECTSLGYGCSCNNLGVRGNVSYVFNMYYQMQDQNPAACGFGGAAKIV 429

Query: 421 TTNTTVGDCLFPIMIK-SEGD--ASAGPSGSPGASPSDTSKSASERLRIAVAAALVGLVL 480
           T N TVGDCLFPI I  S G   AS+ P GSP  SP  + KSA E LR+  A ALVGLV+
Sbjct: 430 TKNATVGDCLFPIQITGSSGSTTASSSPGGSPSGSPGGSGKSAGESLRMRAAVALVGLVV 489

[Close]

BLAST of MC01g0751 vs. ExPASy TrEMBL
Match: A0A6J1BTC0 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111005372 PE=3 SV=1)

HSP 1 Score: 790 bits (2039), Expect = 1.56e-286
Identity = 379/379 (100.00%), Postives = 379/379 (100.00%), Query Frame = 0


Query: 1   IGVNWGTIASHPLEASVVVKMLKDNGIKKVKLFDLDSVAHLSALSGTGIETIVGVPNYRL 60
           IGVNWGTIASHPLEASVVVKMLKDNGIKKVKLFDLDSVAHLSALSGTGIETIVGVPNYRL
Sbjct: 30  IGVNWGTIASHPLEASVVVKMLKDNGIKKVKLFDLDSVAHLSALSGTGIETIVGVPNYRL 89

Query: 61  QKFATNYDYVKDWVKQNISAYLHEGGVDIKYIAVGNEPFLAAYKGDYVNITFPAMQNVQK 120
           QKFATNYDYVKDWVKQNISAYLHEGGVDIKYIAVGNEPFLAAYKGDYVNITFPAMQNVQK
Sbjct: 90  QKFATNYDYVKDWVKQNISAYLHEGGVDIKYIAVGNEPFLAAYKGDYVNITFPAMQNVQK 149

Query: 121 ALDAAGHGDKVKVTTPLNGDVYQSFSNLPSDGKFRADIYDSMKQIVKFLDEHKSPFMVNI 180
           ALDAAGHGDKVKVTTPLNGDVYQSFSNLPSDGKFRADIYDSMKQIVKFLDEHKSPFMVNI
Sbjct: 150 ALDAAGHGDKVKVTTPLNGDVYQSFSNLPSDGKFRADIYDSMKQIVKFLDEHKSPFMVNI 209

Query: 181 YPFINLYQNPGFPFNYAFLDNDGKVKDSVVDDKGTIYYNVLDAHYDTLTWSLKDMGVNDM 240
           YPFINLYQNPGFPFNYAFLDNDGKVKDSVVDDKGTIYYNVLDAHYDTLTWSLKDMGVNDM
Sbjct: 210 YPFINLYQNPGFPFNYAFLDNDGKVKDSVVDDKGTIYYNVLDAHYDTLTWSLKDMGVNDM 269

Query: 241 KIIIGEVGWPTDGNIYANVTLAKRFYNGLFKRVASNKGTPMRPNEKYEIYLFSFLDEDVK 300
           KIIIGEVGWPTDGNIYANVTLAKRFYNGLFKRVASNKGTPMRPNEKYEIYLFSFLDEDVK
Sbjct: 270 KIIIGEVGWPTDGNIYANVTLAKRFYNGLFKRVASNKGTPMRPNEKYEIYLFSFLDEDVK 329

Query: 301 SVDPGNFERHWGIFRYDGTPKFAMDFSGEGVEKMPVAAKGVEYMERKWCVVADNVTDLGG 360
           SVDPGNFERHWGIFRYDGTPKFAMDFSGEGVEKMPVAAKGVEYMERKWCVVADNVTDLGG
Sbjct: 330 SVDPGNFERHWGIFRYDGTPKFAMDFSGEGVEKMPVAAKGVEYMERKWCVVADNVTDLGG 389

Query: 361 DIQKDVSWACEHSDCTSLG 379
           DIQKDVSWACEHSDCTSLG
Sbjct: 390 DIQKDVSWACEHSDCTSLG 408

[Close]

BLAST of MC01g0751 vs. ExPASy TrEMBL
Match: A0A1S3B101 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103484682 PE=3 SV=1)

HSP 1 Score: 618 bits (1594), Expect = 7.64e-218
Identity = 301/477 (63.10%), Postives = 365/477 (76.52%), Query Frame = 0


Query: 1   IGVNWGTIASHPLEASVVVKMLKDNGIKKVKLFDLDSVAHLSALSGTGIETIVGVPNYRL 60
           IG NWG ++SHPL  ++VVK+LKDNGIKKVKLFD DS   +SALSG+ IET++G+PN +L
Sbjct: 29  IGANWGIMSSHPLRPNIVVKLLKDNGIKKVKLFDSDSWT-VSALSGSKIETVIGIPNDQL 88

Query: 61  QKFATNYDYVKDWVKQNISAYLHEGGVDIKYIAVGNEPFLAAYKGDYVNITFPAMQNVQK 120
           +KFA +YD  KDWVK+N++ ++ EGGVD++Y++VGNE FL+AY G YV +T+PAMQN+QK
Sbjct: 89  KKFAESYDDAKDWVKENVTTHMFEGGVDLRYVSVGNEAFLSAYNGTYVKVTYPAMQNIQK 148

Query: 121 ALDAAGHGDKVKVTTPLNGDVYQSFSNLPSDGKFRADIYDSMKQIVKFLDEHKSPFMVNI 180
           ALDAAGHG KVKVTT LN DVY+S +NLPSDG+FR DIY +MK IV FLD +K+PFMVNI
Sbjct: 149 ALDAAGHGKKVKVTTALNADVYESSTNLPSDGQFRPDIYKTMKDIVHFLDRNKAPFMVNI 208

Query: 181 YPFINLYQNPGFPFNYAFLDNDGKVKDSVVDDKGTIYYNVLDAHYDTLTWSLKDMGVNDM 240
           YPF++LYQNP FP +YAF     K      +DKG  Y NV DA+YDTL WSLK +GV DM
Sbjct: 209 YPFLSLYQNPNFPLDYAFFGGGAKA----TNDKGKSYTNVFDANYDTLIWSLKKIGVTDM 268

Query: 241 KIIIGEVGWPTDGNIYANVTLAKRFYNGLFKRVASNKGTPMRPNEKYEIYLFSFLDEDVK 300
           KII+GEVGWPTDGN +ANV LAKRFY+GLFK++AS  GTPMRP EK+E+YLF  LDED+K
Sbjct: 269 KIIVGEVGWPTDGNKFANVELAKRFYDGLFKKLASGNGTPMRPKEKFEVYLFGLLDEDMK 328

Query: 301 SVDPGNFERHWGIFRYDGTPKFAMDFSGEGVEKMPVAAKGVEYMERKWCVVADNVTDLGG 360
           SV PG FERHWGIF++DG PKF MD SG+G +KM VAAKGV+Y+E+KWCV+ +NV +   
Sbjct: 329 SVLPGFFERHWGIFQFDGKPKFPMDMSGKGNDKMLVAAKGVQYLEKKWCVLKENVKNFD- 388

Query: 361 DIQKDVSWACEHSDCTSLGYGCSCNKLDARGNVSYVFNMYYQMQSQNPEACGFGGAAEIV 420
            I K+V +AC  SDCTSLGYG SCN LD RGN+SY FNMYYQMQ Q+ EAC F G+A IV
Sbjct: 389 KISKEVDYACGLSDCTSLGYGSSCNNLDKRGNISYAFNMYYQMQDQSVEACDFSGSAHIV 448

Query: 421 TTNTTVGDCLFPIMIKSEGDASAGPSGSPGASPSDTSKSASERLRIAVAAALVGLVL 477
             N +VG CLFPI I S G                      ERL+ A AAA+VGLVL
Sbjct: 449 KKNASVGSCLFPIQIVSTG----------------------ERLKAAAAAAVVGLVL 477

[Close]

BLAST of MC01g0751 vs. ExPASy TrEMBL
Match: A0A6J1EQB3 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111434805 PE=3 SV=1)

HSP 1 Score: 616 bits (1589), Expect = 4.40e-217
Identity = 284/439 (64.69%), Postives = 356/439 (81.09%), Query Frame = 0


Query: 1   IGVNWGTIASHPLEASVVVKMLKDNGIKKVKLFDLDSVAHLSALSGTGIETIVGVPNYRL 60
           +GVNWGTIASHP+  S+VV +LKDNGIKKVKLFD DS   + AL+G+ IET+V +PN +L
Sbjct: 29  VGVNWGTIASHPINPSIVVNLLKDNGIKKVKLFDSDSWI-MGALAGSKIETVVAIPNDQL 88

Query: 61  QKFATNYDYVKDWVKQNISAYLHEGGVDIKYIAVGNEPFLAAYKGDYVNITFPAMQNVQK 120
           +KFA +YD  KDWVK+N++ Y+++GGVDI+Y+AVGNEPFL  + G Y+ +TFPAMQNVQK
Sbjct: 89  EKFANDYDLAKDWVKENVTTYIYDGGVDIRYVAVGNEPFLTGFNGSYIKLTFPAMQNVQK 148

Query: 121 ALDAAGHGDKVKVTTPLNGDVYQSFSNLPSDGKFRADIYDSMKQIVKFLDEHKSPFMVNI 180
           AL+ AGHGDK+KVTTPLN DVY+S SN PSDG+FR+DI+D+MK IV FL  + +PFMVNI
Sbjct: 149 ALNDAGHGDKIKVTTPLNADVYESPSNQPSDGRFRSDIHDTMKNIVHFLSSNNAPFMVNI 208

Query: 181 YPFINLYQNPGFPFNYAFLDNDGKVKDSVVDDKGTIYYNVLDAHYDTLTWSLKDMGVNDM 240
           YPF++LYQNP FP ++AFLD+ GK     ++DKG  Y NV DA+YDTL WSLK +G  D+
Sbjct: 209 YPFLSLYQNPDFPIDFAFLDSSGKS----INDKGKTYTNVFDANYDTLVWSLKKIGEGDL 268

Query: 241 KIIIGEVGWPTDGNIYANVTLAKRFYNGLFKRVASNKGTPMRPNEKYEIYLFSFLDEDVK 300
           KI++GEVGWPTD N YAN+ LAKRFY+GLFK +AS KGTPMRP+EK EIYLFS  DED+K
Sbjct: 269 KIVVGEVGWPTDANKYANLELAKRFYDGLFKNLASKKGTPMRPDEKLEIYLFSLFDEDMK 328

Query: 301 SVDPGNFERHWGIFRYDGTPKFAMDFSGEGVEKMPVAAKGVEYMERKWCVVADNVTDLGG 360
           SV PG FERHWGIFRYDG PKF MD +G G +KMPVAAKGV+Y+E+KWCV+ +++ DL  
Sbjct: 329 SVLPGFFERHWGIFRYDGKPKFPMDLAGNGNDKMPVAAKGVQYLEKKWCVIKNSIEDLTS 388

Query: 361 DIQKDVSWACEHSDCTSLGYGCSCNKLDARGNVSYVFNMYYQMQSQNPEACGFGGAAEIV 420
           ++ + + +AC  SDCT+L +G SCN L+ARGN S+ +NMY+QMQ Q+ EAC FGG+AEIV
Sbjct: 389 EVSQ-IDYACSLSDCTTLVFGSSCNDLNARGNFSFAYNMYFQMQDQSVEACDFGGSAEIV 448

Query: 421 TTNTTVGDCLFPIMIKSEG 439
           T N +VG CLFPI I S G
Sbjct: 449 TKNASVGTCLFPIQIVSAG 461

[Close]

BLAST of MC01g0751 vs. TAIR 10
Match: AT5G64790.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 528.9 bits (1361), Expect = 4.4e-150
Identity = 246/439 (56.04%), Postives = 324/439 (73.80%), Query Frame = 0


Query: 2   GVNWGTIASHPLEASVVVKMLKDNGIKKVKLFDLDSVAHLSALSGTGIETIVGVPNYRLQ 61
           GVNWG IASHPL  ++VV+MLKDN I KVKLFD DS   ++AL+GTG+E +VG+PN  L+
Sbjct: 29  GVNWGNIASHPLNPNIVVQMLKDNKINKVKLFDADSWT-MNALAGTGMEVMVGIPNNLLE 88

Query: 62  KFATNYDYVKDWVKQNISAYLHEGGVDIKYIAVGNEPFLAAYKGDYVNITFPAMQNVQKA 121
             A +YD  KDWVK+N++ Y+ +GGVDIKY+AVGNEPFL+AY G ++  TFPA++N+ KA
Sbjct: 89  SLADDYDNAKDWVKENVTQYIRKGGVDIKYVAVGNEPFLSAYNGSFLKTTFPALKNIHKA 148

Query: 122 LDAAGHGDKVKVTTPLNGDVYQSFSNLPSDGKFRADIYDSMKQIVKFLDEHKSPFMVNIY 181
           L  AGH + +K T P N +VYQS ++ PS+G FR D+  +M  IV F  E+  PF VNIY
Sbjct: 149 LKEAGHTNIMKATIPQNAEVYQSANDKPSEGDFRKDVKQTMLDIVNFFHENDLPFTVNIY 208

Query: 182 PFINLYQNPGFPFNYAFLDNDGKVKDSVVDDKGTIYYNVLDAHYDTLTWSLKDMGVNDMK 241
           PF++LY N  FP  +AFLD DG+     + DKG  Y NV DA+YDTL ++LK  G++DMK
Sbjct: 209 PFLSLYLNEHFPVEFAFLDGDGQ----TMTDKGKNYDNVFDANYDTLVYALKKAGIHDMK 268

Query: 242 IIIGEVGWPTDGNIYANVTLAKRFYNGLFKRVASNKGTPMRPNEKYEIYLFSFLDEDVKS 301
           II+GEVGWPTDG+ YA+  LA++FY GL KR+A + GTP RP E+ E+YLF FLDED+KS
Sbjct: 269 IIVGEVGWPTDGHKYASPKLAEKFYAGLMKRLAKDGGTPTRP-ERLEVYLFGFLDEDMKS 328

Query: 302 VDPGNFERHWGIFRYDGTPKFAMDFSGEGVEKMPVAAKGVEYMERKWCVVADNVTDLGGD 361
           + PG FERHWGIFRYDGTPKF +DF+G+G + +PVAAKGV+Y+E++WCVV  +  +L  +
Sbjct: 329 ILPGPFERHWGIFRYDGTPKFMLDFTGQGRQMVPVAAKGVQYLEKQWCVVNKDTVNL-DE 388

Query: 362 IQKDVSWACEHSDCTSLGYGCSCNKLDARGNVSYVFNMYYQMQSQNPEACGFGGAAEIVT 421
           +  D+ +AC H DCT++  G +C+KL    N+SY FNMY+Q+Q Q+  AC F GAA I  
Sbjct: 389 VGPDLDYACYHGDCTAMEAGSTCSKLTKVQNISYAFNMYFQIQDQDVRACDFKGAAMITK 448

Query: 422 TNTTVGDCLFPIMIKSEGD 441
            N +VG CLFP+ I S  D
Sbjct: 449 VNASVGSCLFPVQIVSGSD 460

[Close]

BLAST of MC01g0751 vs. TAIR 10
Match: AT3G04010.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 481.1 bits (1237), Expect = 1.0e-135
Identity = 234/446 (52.47%), Postives = 304/446 (68.16%), Query Frame = 0


Query: 1   IGVNWGTIASHPLEASVVVKMLKDNGIKKVKLFDLDSVAHLSALSGTGIETIVGVPNYRL 60
           +GVNWGT+ASH L    VV+MLKDN I+KVKLFD D+   + AL+G+G+E +V +PN  L
Sbjct: 33  LGVNWGTMASHQLPPKTVVEMLKDNNIQKVKLFDADTNT-MGALAGSGVEVMVAIPNDLL 92

Query: 61  QKFATNYDYVKDWVKQNISAYLHEGGVDIKYIAVGNEPFLAAYKGDYVNITFPAMQNVQK 120
                NY   KDWV++N+S +    GV IKY+AVGNEPFL AY G ++N+T+PA+ N+Q 
Sbjct: 93  LAMG-NYQRAKDWVQRNVSRFNFNNGVKIKYVAVGNEPFLTAYNGSFINLTYPALFNIQT 152

Query: 121 ALDAAGHGDKVKVTTPLNGDVYQS--FSNLPSDGKFRADIYDSMKQIVKFLDEHKSPFMV 180
           AL+ AG GD  K T PLN DVY S   + +PS G+FR+DI   M QIV FL ++K+PF V
Sbjct: 153 ALNEAGVGDFTKATVPLNADVYNSPPDNQVPSAGRFRSDIIQEMTQIVNFLAQNKAPFTV 212

Query: 181 NIYPFINLYQNPGFPFNYAFLDNDGKVKDSVVDDKGTIYYNVLDAHYDTLTWSLKDMGVN 240
           NIYPF++LY +  FPF YAF D       + V+D G IY NV DA +DTL  SL  +   
Sbjct: 213 NIYPFLSLYLSSDFPFEYAFFDG-----QNTVNDNGVIYTNVFDASFDTLLASLNALNHG 272

Query: 241 DMKIIIGEVGWPTDGNIYANVTLAKRFYNGLFKRVASNKGTPMRPNEKYEIYLFSFLDED 300
           +M++I+GEVGWPTDG+  ANV  A+RFY+GL  R+A+N GTPMR     E+YLF F+DED
Sbjct: 273 NMEVIVGEVGWPTDGDKNANVPNAERFYSGLLPRLANNVGTPMRKG-YIEVYLFGFIDED 332

Query: 301 VKSVDPGNFERHWGIFRYDGTPKFAMDFSGEGVEKMPVAAKGVEYMERKWCVVADNVTDL 360
            KSV PGNFERHWGIF++DG PKF +DF G+G +K    A+ V+Y   +WC+   N    
Sbjct: 333 AKSVAPGNFERHWGIFKFDGQPKFPVDFRGQGQKKFLTGAQNVQYFLNQWCMFNPNGRGN 392

Query: 361 GGDIQKDVSWACEHSDCTSLGYGCSCNKLDARGNVSYVFNMYYQMQSQNPEACGFGGAAE 420
              +  ++++AC HSDCT+LGYG SC  LDA GN SY FNMY+Q+Q+Q  +AC F G A 
Sbjct: 393 MSRLGDNINYACSHSDCTALGYGSSCGNLDANGNASYAFNMYFQVQNQEAQACDFEGLAT 452

Query: 421 IVTTNTTVGDCLFPIMIKSEGDASAG 445
           I T N + G C FPI I   G+ S+G
Sbjct: 453 ITTQNISQGQCNFPIQI---GEPSSG 467

[Close]

BLAST of MC01g0751 vs. TAIR 10
Match: AT2G19440.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 480.3 bits (1235), Expect = 1.8e-135
Identity = 234/448 (52.23%), Postives = 302/448 (67.41%), Query Frame = 0


Query: 1   IGVNWGTIASHPLEASVVVKMLKDNGIKKVKLFDLDSVAHLSALSGTGIETIVGVPNYRL 60
           +GVNWGT+A+H L    VV+MLKDN I KVKLFD D    +SALSG+G+E +V +PN +L
Sbjct: 21  LGVNWGTMATHKLPPKKVVQMLKDNNINKVKLFDADETT-MSALSGSGLEVMVAIPNDQL 80

Query: 61  QKFATNYDYVKDWVKQNISAYLHEGGVDIKYIAVGNEPFLAAYKGDYVNITFPAMQNVQK 120
            K   +YD  KDWV +N++ Y   GGV+I ++AVGNEPFL +Y G ++N+TFPA+QN+Q 
Sbjct: 81  -KVMGSYDRAKDWVHKNVTRYNFNGGVNITFVAVGNEPFLKSYNGSFINLTFPALQNIQN 140

Query: 121 ALDAAGHGDKVKVTTPLNGDVYQSFSN--LPSDGKFRADIYDSMKQIVKFLDEHKSPFMV 180
           AL+ AG G  VK T PLN DVY S S+  +PS G+FR DI   M QIV FL  + +P  +
Sbjct: 141 ALNEAGLGSSVKATVPLNADVYDSPSSNPVPSAGRFRPDIIGQMTQIVDFLGNNSAPITI 200

Query: 181 NIYPFINLYQNPGFPFNYAFLDNDGKVKDSVVDDKGTIYYNVLDAHYDTLTWSLKDMGVN 240
           NIYPF++LY N  FP NYAF D         VDD G  Y NV DA++DTL  +LK +G  
Sbjct: 201 NIYPFLSLYGNDDFPLNYAFFDG-----AKPVDDNGIAYTNVFDANFDTLVSALKAVGHG 260

Query: 241 DMKIIIGEVGWPTDGNIYANVTLAKRFYNGLFKRVASNKGTPMRPNEKYEIYLFSFLDED 300
           DM II+GEVGWPT+G+ +AN   A RFYNGL  R+  N+GTP+RP    E+YLF  LDED
Sbjct: 261 DMPIIVGEVGWPTEGDKHANSGSAYRFYNGLLPRLGENRGTPLRPT-YIEVYLFGLLDED 320

Query: 301 VKSVDPGNFERHWGIFRYDGTPKFAMDFSGEGVEKMPVAAKGVEYMERKWCVVADNVTDL 360
            KS+ PG FERHWGIF++DG PKF +D SG+G  K+ + A+ V Y  +KWC+      DL
Sbjct: 321 AKSIAPGEFERHWGIFKFDGQPKFPIDLSGQGQNKLLIGAENVTYQPKKWCMFNTEAKDL 380

Query: 361 GGDIQKDVSWACEHSDCTSLGYGCSCNKLDARGNVSYVFNMYYQMQSQNPEACGFGGAAE 420
              +  ++ +AC  SDCT+LGYG SCN LDA GN SY FNMY+Q+++Q+ +AC F G A 
Sbjct: 381 -TKLAANIDYACTFSDCTALGYGSSCNTLDANGNASYAFNMYFQVKNQDEDACIFQGLAT 440

Query: 421 IVTTNTTVGDCLFPIMIKSEGDASAGPS 447
           I T N + G C FPI I +   +S   S
Sbjct: 441 ITTKNISQGQCNFPIQIVASTASSFSSS 459

[Close]

BLAST of MC01g0751 vs. TAIR 10
Match: AT1G64760.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 479.2 bits (1232), Expect = 4.0e-135
Identity = 229/444 (51.58%), Postives = 303/444 (68.24%), Query Frame = 0


Query: 1   IGVNWGTIASHPLEASVVVKMLKDNGIKKVKLFDLDSVAHLSALSGTGIETIVGVPNYRL 60
           +GVNWGT+A+H L    VV+MLKDN I KVKLFD D    + AL+G+G+E +V +PN +L
Sbjct: 25  LGVNWGTMATHKLPPKTVVQMLKDNNINKVKLFDADETT-MGALAGSGLEVMVAIPNDQL 84

Query: 61  QKFATNYDYVKDWVKQNISAYLHEGGVDIKYIAVGNEPFLAAYKGDYVNITFPAMQNVQK 120
            K  T+YD  KDWV++N++ Y  +GGV+I ++AVGNEPFL +Y G ++N+TFPA+ N+Q 
Sbjct: 85  -KVMTSYDRAKDWVRKNVTRYNFDGGVNITFVAVGNEPFLKSYNGSFINLTFPALANIQN 144

Query: 121 ALDAAGHGDKVKVTTPLNGDVYQSFSN--LPSDGKFRADIYDSMKQIVKFLDEHKSPFMV 180
           AL+ AG G+ VK T PLN DVY S ++  +PS G+FR DI   M QIV FL ++ +P  +
Sbjct: 145 ALNEAGLGNSVKATVPLNADVYDSPASNPVPSAGRFRPDIIGQMTQIVDFLGKNNAPITI 204

Query: 181 NIYPFINLYQNPGFPFNYAFLDNDGKVKDSVVDDKGTIYYNVLDAHYDTLTWSLKDMGVN 240
           NIYPF++LY N  FP NYAF D    + D+ +D     Y NV DA++DTL  SLK +G  
Sbjct: 205 NIYPFLSLYGNDDFPLNYAFFDGAEPINDNGID-----YTNVFDANFDTLVSSLKAVGHG 264

Query: 241 DMKIIIGEVGWPTDGNIYANVTLAKRFYNGLFKRVASNKGTPMRPNEKYEIYLFSFLDED 300
           DM II+GEVGWPT+G+ +AN   A RFYNGL  R+ +NKGTP+RP    E+YLF  LDED
Sbjct: 265 DMPIIVGEVGWPTEGDKHANAGSAYRFYNGLLPRLGTNKGTPLRPT-YIEVYLFGLLDED 324

Query: 301 VKSVDPGNFERHWGIFRYDGTPKFAMDFSGEGVEKMPVAAKGVEYMERKWCVVADNVTDL 360
            KS+ PG FERHWGIF++DG PKF +D SG+G  K  + A+ V Y+  KWC       DL
Sbjct: 325 AKSIAPGPFERHWGIFKFDGQPKFPIDLSGQGQSKFLIGAQNVPYLPNKWCTFNPEAKDL 384

Query: 361 GGDIQKDVSWACEHSDCTSLGYGCSCNKLDARGNVSYVFNMYYQMQSQNPEACGFGGAAE 420
              +  ++ +AC  SDCT+LGYG SCN LDA GN SY FNM++Q+++Q+  AC F G A 
Sbjct: 385 -TKLAANIDYACTFSDCTALGYGSSCNTLDANGNASYAFNMFFQVKNQDESACYFQGLAT 444

Query: 421 IVTTNTTVGDCLFPIMIKSEGDAS 443
           I T N + G C FPI I +   +S
Sbjct: 445 ITTQNISQGQCNFPIQIVASSASS 459

[Close]

BLAST of MC01g0751 vs. TAIR 10
Match: AT1G64760.2 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 479.2 bits (1232), Expect = 4.0e-135
Identity = 229/444 (51.58%), Postives = 303/444 (68.24%), Query Frame = 0


Query: 1   IGVNWGTIASHPLEASVVVKMLKDNGIKKVKLFDLDSVAHLSALSGTGIETIVGVPNYRL 60
           +GVNWGT+A+H L    VV+MLKDN I KVKLFD D    + AL+G+G+E +V +PN +L
Sbjct: 25  LGVNWGTMATHKLPPKTVVQMLKDNNINKVKLFDADETT-MGALAGSGLEVMVAIPNDQL 84

Query: 61  QKFATNYDYVKDWVKQNISAYLHEGGVDIKYIAVGNEPFLAAYKGDYVNITFPAMQNVQK 120
            K  T+YD  KDWV++N++ Y  +GGV+I ++AVGNEPFL +Y G ++N+TFPA+ N+Q 
Sbjct: 85  -KVMTSYDRAKDWVRKNVTRYNFDGGVNITFVAVGNEPFLKSYNGSFINLTFPALANIQN 144

Query: 121 ALDAAGHGDKVKVTTPLNGDVYQSFSN--LPSDGKFRADIYDSMKQIVKFLDEHKSPFMV 180
           AL+ AG G+ VK T PLN DVY S ++  +PS G+FR DI   M QIV FL ++ +P  +
Sbjct: 145 ALNEAGLGNSVKATVPLNADVYDSPASNPVPSAGRFRPDIIGQMTQIVDFLGKNNAPITI 204

Query: 181 NIYPFINLYQNPGFPFNYAFLDNDGKVKDSVVDDKGTIYYNVLDAHYDTLTWSLKDMGVN 240
           NIYPF++LY N  FP NYAF D    + D+ +D     Y NV DA++DTL  SLK +G  
Sbjct: 205 NIYPFLSLYGNDDFPLNYAFFDGAEPINDNGID-----YTNVFDANFDTLVSSLKAVGHG 264

Query: 241 DMKIIIGEVGWPTDGNIYANVTLAKRFYNGLFKRVASNKGTPMRPNEKYEIYLFSFLDED 300
           DM II+GEVGWPT+G+ +AN   A RFYNGL  R+ +NKGTP+RP    E+YLF  LDED
Sbjct: 265 DMPIIVGEVGWPTEGDKHANAGSAYRFYNGLLPRLGTNKGTPLRPT-YIEVYLFGLLDED 324

Query: 301 VKSVDPGNFERHWGIFRYDGTPKFAMDFSGEGVEKMPVAAKGVEYMERKWCVVADNVTDL 360
            KS+ PG FERHWGIF++DG PKF +D SG+G  K  + A+ V Y+  KWC       DL
Sbjct: 325 AKSIAPGPFERHWGIFKFDGQPKFPIDLSGQGQSKFLIGAQNVPYLPNKWCTFNPEAKDL 384

Query: 361 GGDIQKDVSWACEHSDCTSLGYGCSCNKLDARGNVSYVFNMYYQMQSQNPEACGFGGAAE 420
              +  ++ +AC  SDCT+LGYG SCN LDA GN SY FNM++Q+++Q+  AC F G A 
Sbjct: 385 -TKLAANIDYACTFSDCTALGYGSSCNTLDANGNASYAFNMFFQVKNQDESACYFQGLAT 444

Query: 421 IVTTNTTVGDCLFPIMIKSEGDAS 443
           I T N + G C FPI I +   +S
Sbjct: 445 ITTQNISQGQCNFPIQIVASSASS 459

The following BLAST results are available for this feature:

BLAST of MC01g0751 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6NKW9	5.6e-134	51.58	Glucan endo-1,3-beta-glucosidase 8 OS=Arabidopsis thaliana OX=3702 GN=At1g64760 ...	[more]
Q9M088	4.7e-133	52.44	Glucan endo-1,3-beta-glucosidase 5 OS=Arabidopsis thaliana OX=3702 GN=At4g31140 ...	[more]
Q93Z08	1.9e-126	50.46	Glucan endo-1,3-beta-glucosidase 6 OS=Arabidopsis thaliana OX=3702 GN=At5g58090 ...	[more]
Q9FGH4	4.3e-86	38.93	Glucan endo-1,3-beta-glucosidase 9 OS=Arabidopsis thaliana OX=3702 GN=At5g58480 ...	[more]
Q9M069	5.2e-55	31.30	Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 ...	[more]

BLAST of MC01g0751 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022132538.1	0.0	100.00	glucan endo-1,3-beta-glucosidase 5-like isoform X1 [Momordica charantia]	[more]
XP_022133125.1	2.87e-293	79.92	glucan endo-1,3-beta-glucosidase 8-like, partial [Momordica charantia]	[more]
XP_022132539.1	3.21e-286	100.00	glucan endo-1,3-beta-glucosidase 5-like isoform X2 [Momordica charantia]	[more]
XP_038883272.1	7.30e-218	63.39	glucan endo-1,3-beta-glucosidase 8 [Benincasa hispida]	[more]
XP_008440124.1	1.58e-217	63.10	PREDICTED: glucan endo-1,3-beta-glucosidase 8 [Cucumis melo]	[more]

BLAST of MC01g0751 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BWJ0	0.0	100.00	(1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111005372 ...	[more]
A0A6J1BUF0	1.39e-293	79.92	(1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111005798 ...	[more]
A0A6J1BTC0	1.56e-286	100.00	(1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111005372 ...	[more]
A0A1S3B101	7.64e-218	63.10	(1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103484682 PE=3 SV...	[more]
A0A6J1EQB3	4.40e-217	64.69	(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111434805 P...	[more]

BLAST of MC01g0751 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G64790.1	4.4e-150	56.04	O-Glycosyl hydrolases family 17 protein	[more]
AT3G04010.1	1.0e-135	52.47	O-Glycosyl hydrolases family 17 protein	[more]
AT2G19440.1	1.8e-135	52.23	O-Glycosyl hydrolases family 17 protein	[more]
AT1G64760.1	4.0e-135	51.58	O-Glycosyl hydrolases family 17 protein	[more]
AT1G64760.2	4.0e-135	51.58	O-Glycosyl hydrolases family 17 protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012946	X8 domain	SMART	SM00768	X8_cls	coord: 347..431 e-value: 4.0E-31 score: 119.4
IPR012946	X8 domain	PFAM	PF07983	X8	coord: 347..417 e-value: 4.6E-16 score: 59.3
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 1..332 e-value: 7.7E-108 score: 362.2
None	No IPR available	GENE3D	1.20.58.1040		coord: 342..435 e-value: 3.6E-6 score: 29.2
None	No IPR available	PANTHER	PTHR32227:SF290	BETA-1,3-GLUCANASE	coord: 1..441
IPR000490	Glycoside hydrolase family 17	PFAM	PF00332	Glyco_hydro_17	coord: 1..326 e-value: 3.7E-61 score: 207.4
IPR000490	Glycoside hydrolase family 17	PROSITE	PS00587	GLYCOSYL_HYDROL_F17	coord: 240..253
IPR044965	Glycoside hydrolase family 17, plant	PANTHER	PTHR32227	GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED	coord: 1..441
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 1..326

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC01g0751.1	MC01g0751.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
biological_process	GO:0008033	tRNA processing
cellular_component	GO:0046658	anchored component of plasma membrane
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0042973	glucan endo-1,3-beta-D-glucosidase activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0000166	nucleotide binding
molecular_function	GO:0016779	nucleotidyltransferase activity
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds