MC01g0690 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC01g0690
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionCysteine proteinase inhibitor
LocationMC01: 13077706 .. 13077996 (-)
RNA-Seq ExpressionMC01g0690
SyntenyMC01g0690
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GAACCGATCAAGGACATCAACAATCCAAGGGTGCAAGAGGCAGGAAGGTGTGCAGTGATGGAGCATAACAGCCAAACTGGGGAGAACCTGAAGTTCACTCGCGTGGTGAATGGTCTTAGACAGAGGCTGCCATTTCTTCCCATTATTTGTCCATTCGAAGCATGGAAGCTGCACCTTGTGGTGGAGGCCGTAAACAGTGATGGAATTGCTTGGACATATGAGACTGAGGTGTTGGATATTGCATTTGCAGGCATCTTCAAGCTCCTTTCTTTCAAGGAAATTCTCCACTAC

mRNA sequence

GAACCGATCAAGGACATCAACAATCCAAGGGTGCAAGAGGCAGGAAGGTGTGCAGTGATGGAGCATAACAGCCAAACTGGGGAGAACCTGAAGTTCACTCGCGTGGTGAATGGTCTTAGACAGAGGCTGCCATTTCTTCCCATTATTTGTCCATTCGAAGCATGGAAGCTGCACCTTGTGGTGGAGGCCGTAAACAGTGATGGAATTGCTTGGACATATGAGACTGAGGTGTTGGATATTGCATTTGCAGGCATCTTCAAGCTCCTTTCTTTCAAGGAAATTCTCCACTAC

Coding sequence (CDS)

GAACCGATCAAGGACATCAACAATCCAAGGGTGCAAGAGGCAGGAAGGTGTGCAGTGATGGAGCATAACAGCCAAACTGGGGAGAACCTGAAGTTCACTCGCGTGGTGAATGGTCTTAGACAGAGGCTGCCATTTCTTCCCATTATTTGTCCATTCGAAGCATGGAAGCTGCACCTTGTGGTGGAGGCCGTAAACAGTGATGGAATTGCTTGGACATATGAGACTGAGGTGTTGGATATTGCATTTGCAGGCATCTTCAAGCTCCTTTCTTTCAAGGAAATTCTCCACTAC

Protein sequence

EPIKDINNPRVQEAGRCAVMEHNSQTGENLKFTRVVNGLRQRLPFLPIICPFEAWKLHLVVEAVNSDGIAWTYETEVLDIAFAGIFKLLSFKEILHY
Homology
BLAST of MC01g0690 vs. ExPASy Swiss-Prot
Match: Q10J94 (Cysteine proteinase inhibitor 8 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0429000 PE=2 SV=1)

HSP 1 Score: 45.1 bits (105), Expect = 5.4e-04
Identity = 28/91 (30.77%), Postives = 43/91 (47.25%), Query Frame = 0

Query: 2   PIKDINNPRVQEAGRCAVMEHNSQTGENLKFTRVVNGLRQRLPFLPIICPFEAWKLHLVV 61
           PI D+ +P +QE G  AV  H S + + L+F RV +G +Q +               LVV
Sbjct: 37  PITDVGDPHIQELGGWAVERHASLSSDGLRFRRVTSGEQQVV---------SGMNYRLVV 96

Query: 62  EAVNSDGIAWTYETEVLDIAFAGIFKLLSFK 93
            A +  G   +Y   V + ++    +L SFK
Sbjct: 97  SASDPAGATASYVAVVYEQSWTNTRQLTSFK 118

BLAST of MC01g0690 vs. NCBI nr
Match: KAE8649716.1 (hypothetical protein Csa_012476 [Cucumis sativus])

HSP 1 Score: 80.1 bits (196), Expect = 5.56e-17
Identity = 46/101 (45.54%), Postives = 60/101 (59.41%), Query Frame = 0

Query: 1   EPIKDINNPRVQEAGRCAVMEHNSQTGENLKFTRVVNGLRQRLPFLPIICPFEAWKLHLV 60
           EPIKDIN+P+VQE G   V +HN +TGENLKF RVVNGL     F+    P       LV
Sbjct: 8   EPIKDINDPQVQEIGNLTVDDHNKKTGENLKFVRVVNGLFAG--FVDPFSPLGGTVYELV 67

Query: 61  VEAVNSDGIAWTYETEV---LDIAFAGI-FKLLSFKEILHY 97
           +EA+N+  I WTY+ +V     I   G+ +   SF+ +L Y
Sbjct: 68  LEAINAGKINWTYKVKVSASTTIRPLGVVYNFQSFEPVLQY 106

BLAST of MC01g0690 vs. NCBI nr
Match: XP_038897049.1 (cysteine proteinase inhibitor 5-like [Benincasa hispida])

HSP 1 Score: 74.3 bits (181), Expect = 1.18e-14
Identity = 42/95 (44.21%), Postives = 55/95 (57.89%), Query Frame = 0

Query: 1   EPIKDINNPRVQEAGRCAVMEHNSQTGENLKFTRVVNGLRQRLPFLPIICPFEAWKLHLV 60
           EPI++I +P VQE GR +VMEHN QTG NLKF RVVNG +Q +  +            L 
Sbjct: 28  EPIENIEDPYVQEMGRFSVMEHNKQTGANLKFIRVVNGEKQVVAGI---------NYRLR 87

Query: 61  VEAVNSDGIAWTYETEVLDIAFAGIFKLLSFKEIL 95
           +EA N   + WTYE  V D  +   +KL+ F  +L
Sbjct: 88  IEARNEMNLVWTYEAMVNDRPWEKKWKLIYFVPLL 113

BLAST of MC01g0690 vs. NCBI nr
Match: XP_008442277.1 (PREDICTED: cysteine proteinase inhibitor A-like [Cucumis melo] >KAA0041897.1 cysteine proteinase inhibitor A-like [Cucumis melo var. makuwa] >TYK05422.1 cysteine proteinase inhibitor A-like [Cucumis melo var. makuwa])

HSP 1 Score: 71.2 bits (173), Expect = 1.76e-13
Identity = 43/103 (41.75%), Postives = 56/103 (54.37%), Query Frame = 0

Query: 1   EPIKDIN-NPRVQEAGRCAVMEHNSQTGENLKFTRVVNGLRQRLPFLPIICPFEAWKLHL 60
           +PI+DIN N  VQ+ G   V +HN +TGENLKF RVVNGL     F   I P       L
Sbjct: 8   KPIEDINDNLEVQDLGNLTVDDHNKKTGENLKFVRVVNGLAAS--FSDPISPLGGVLYEL 67

Query: 61  VVEAVNSDGIAWTYETEV-----LDIAFAGIFKLLSFKEILHY 97
           V+EA+ +  I WTY+  V     +   F  ++   SFK +L Y
Sbjct: 68  VLEAITTSKINWTYKARVSKSKAITPPFHMLYSFQSFKPVLEY 108

BLAST of MC01g0690 vs. NCBI nr
Match: XP_022975718.1 (cysteine proteinase inhibitor 6-like [Cucurbita maxima])

HSP 1 Score: 69.7 bits (169), Expect = 7.61e-13
Identity = 40/91 (43.96%), Postives = 58/91 (63.74%), Query Frame = 0

Query: 2   PIKDINNPRVQEAGRCAVMEHNSQTGENLKFTRVVNGLRQRLPFLPIICPFEAWKLHLVV 61
           PIKDIN+P VQE GR AVMEH+ Q+ E++ FT+VV+G  Q       +   +A++L +++
Sbjct: 28  PIKDINDPYVQELGRFAVMEHDMQSKEDVTFTKVVSGATQ-------VVAGKAYRL-VIM 87

Query: 62  EAVNSDGIAWTYETEVLDIAFAGIFKLLSFK 92
              N + +   YE  VLDI +   +KLLSFK
Sbjct: 88  GVSNGEKMFSYYEAVVLDIPWEHSWKLLSFK 110

BLAST of MC01g0690 vs. NCBI nr
Match: KAE8648508.1 (hypothetical protein Csa_009101, partial [Cucumis sativus])

HSP 1 Score: 68.6 bits (166), Expect = 2.74e-12
Identity = 39/95 (41.05%), Postives = 55/95 (57.89%), Query Frame = 0

Query: 2   PIKDINNPRVQEAGRCAVMEHNSQTGENLKFTRVVNGLRQRLPFLPIICPFEAWKLHLVV 61
           PIKD+++  ++  G  AV EHN  TGENL+F +V+NGL Q      I          LV+
Sbjct: 33  PIKDVDSEEIRGFGEFAVSEHNEATGENLEFVKVINGLEQGRRGFRI-------DFTLVL 92

Query: 62  EAVNSDGIAWTYETEVLDIAFAGIFKLLSFKEILH 96
            A NS GI WTY+ +V     AG+ +LL F+ +L+
Sbjct: 93  SAKNSIGIIWTYKVKVFLNQRAGV-RLLEFEAVLN 119

BLAST of MC01g0690 vs. ExPASy TrEMBL
Match: A0A5A7TJK0 (Cysteine proteinase inhibitor A-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold83G001140 PE=4 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 8.53e-14
Identity = 43/103 (41.75%), Postives = 56/103 (54.37%), Query Frame = 0

Query: 1   EPIKDIN-NPRVQEAGRCAVMEHNSQTGENLKFTRVVNGLRQRLPFLPIICPFEAWKLHL 60
           +PI+DIN N  VQ+ G   V +HN +TGENLKF RVVNGL     F   I P       L
Sbjct: 8   KPIEDINDNLEVQDLGNLTVDDHNKKTGENLKFVRVVNGLAAS--FSDPISPLGGVLYEL 67

Query: 61  VVEAVNSDGIAWTYETEV-----LDIAFAGIFKLLSFKEILHY 97
           V+EA+ +  I WTY+  V     +   F  ++   SFK +L Y
Sbjct: 68  VLEAITTSKINWTYKARVSKSKAITPPFHMLYSFQSFKPVLEY 108

BLAST of MC01g0690 vs. ExPASy TrEMBL
Match: A0A1S3B5B9 (cysteine proteinase inhibitor A-like OS=Cucumis melo OX=3656 GN=LOC103486185 PE=4 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 8.53e-14
Identity = 43/103 (41.75%), Postives = 56/103 (54.37%), Query Frame = 0

Query: 1   EPIKDIN-NPRVQEAGRCAVMEHNSQTGENLKFTRVVNGLRQRLPFLPIICPFEAWKLHL 60
           +PI+DIN N  VQ+ G   V +HN +TGENLKF RVVNGL     F   I P       L
Sbjct: 8   KPIEDINDNLEVQDLGNLTVDDHNKKTGENLKFVRVVNGLAAS--FSDPISPLGGVLYEL 67

Query: 61  VVEAVNSDGIAWTYETEV-----LDIAFAGIFKLLSFKEILHY 97
           V+EA+ +  I WTY+  V     +   F  ++   SFK +L Y
Sbjct: 68  VLEAITTSKINWTYKARVSKSKAITPPFHMLYSFQSFKPVLEY 108

BLAST of MC01g0690 vs. ExPASy TrEMBL
Match: A0A6J1IK33 (cysteine proteinase inhibitor 6-like OS=Cucurbita maxima OX=3661 GN=LOC111475798 PE=4 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 3.69e-13
Identity = 40/91 (43.96%), Postives = 58/91 (63.74%), Query Frame = 0

Query: 2   PIKDINNPRVQEAGRCAVMEHNSQTGENLKFTRVVNGLRQRLPFLPIICPFEAWKLHLVV 61
           PIKDIN+P VQE GR AVMEH+ Q+ E++ FT+VV+G  Q       +   +A++L +++
Sbjct: 28  PIKDINDPYVQELGRFAVMEHDMQSKEDVTFTKVVSGATQ-------VVAGKAYRL-VIM 87

Query: 62  EAVNSDGIAWTYETEVLDIAFAGIFKLLSFK 92
              N + +   YE  VLDI +   +KLLSFK
Sbjct: 88  GVSNGEKMFSYYEAVVLDIPWEHSWKLLSFK 110

BLAST of MC01g0690 vs. ExPASy TrEMBL
Match: A0A0A0KRZ8 (Cystatin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G487690 PE=4 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 8.80e-13
Identity = 39/95 (41.05%), Postives = 55/95 (57.89%), Query Frame = 0

Query: 2   PIKDINNPRVQEAGRCAVMEHNSQTGENLKFTRVVNGLRQRLPFLPIICPFEAWKLHLVV 61
           PIKD+++  ++  G  AV EHN  TGENL+F +V+NGL Q      I          LV+
Sbjct: 16  PIKDVDSEEIRGFGEFAVSEHNEATGENLEFVKVINGLEQGRRGFRI-------DFTLVL 75

Query: 62  EAVNSDGIAWTYETEVLDIAFAGIFKLLSFKEILH 96
            A NS GI WTY+ +V     AG+ +LL F+ +L+
Sbjct: 76  SAKNSIGIIWTYKVKVFLNQRAGV-RLLEFEAVLN 102

BLAST of MC01g0690 vs. ExPASy TrEMBL
Match: A0A5D3C2L1 (Proteinase inhibitor I25, cystatin OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold83G001230 PE=4 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 1.85e-12
Identity = 37/82 (45.12%), Postives = 54/82 (65.85%), Query Frame = 0

Query: 1  EPIKDINNP-RVQEA-GRCAVMEHNSQTGENLKFTRVVNGLRQRLPFLPIICPFEAWKLH 60
          +PI++IN+  +VQ   G+ AV +HN +TGENL+F  VVNGLR  + F+   C  E    H
Sbjct: 11 KPIENINDDLQVQSIIGKLAVEDHNKKTGENLEFVDVVNGLRSEV-FVQPGCTHEGILYH 70

Query: 61 LVVEAVNSDGIAWTYETEVLDI 80
          L+VEA   +GI WTY  ++L++
Sbjct: 71 LLVEAKTIEGINWTYVAKLLEL 91

BLAST of MC01g0690 vs. TAIR 10
Match: AT4G16500.1 (Cystatin/monellin superfamily protein )

HSP 1 Score: 41.6 bits (96), Expect = 4.3e-04
Identity = 27/94 (28.72%), Postives = 44/94 (46.81%), Query Frame = 0

Query: 1   EPIKDINNPRVQEAGRCAVMEHNSQTGENLKFTRVVNGLRQRLPFLPIICPFEAWKLHLV 60
           +PIK++++P V    + A+ EHN ++ E L F +VV G  Q +            K  L 
Sbjct: 33  KPIKNVSDPDVVAVAKYAIEEHNKESKEKLVFVKVVEGTTQVV---------SGTKYDLK 92

Query: 61  VEAVNSDGIAWTYETEVLDIAFAGIFKLLSFKEI 95
           + A +  G    YE  V++  +     L SFK +
Sbjct: 93  IAAKDGGGKIKNYEAVVVEKLWLHSKSLESFKAL 117

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q10J945.4e-0430.77Cysteine proteinase inhibitor 8 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03... [more]
Match NameE-valueIdentityDescription
KAE8649716.15.56e-1745.54hypothetical protein Csa_012476 [Cucumis sativus][more]
XP_038897049.11.18e-1444.21cysteine proteinase inhibitor 5-like [Benincasa hispida][more]
XP_008442277.11.76e-1341.75PREDICTED: cysteine proteinase inhibitor A-like [Cucumis melo] >KAA0041897.1 cys... [more]
XP_022975718.17.61e-1343.96cysteine proteinase inhibitor 6-like [Cucurbita maxima][more]
KAE8648508.12.74e-1241.05hypothetical protein Csa_009101, partial [Cucumis sativus][more]
Match NameE-valueIdentityDescription
A0A5A7TJK08.53e-1441.75Cysteine proteinase inhibitor A-like OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A1S3B5B98.53e-1441.75cysteine proteinase inhibitor A-like OS=Cucumis melo OX=3656 GN=LOC103486185 PE=... [more]
A0A6J1IK333.69e-1343.96cysteine proteinase inhibitor 6-like OS=Cucurbita maxima OX=3661 GN=LOC111475798... [more]
A0A0A0KRZ88.80e-1341.05Cystatin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G487690 PE... [more]
A0A5D3C2L11.85e-1245.12Proteinase inhibitor I25, cystatin OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
Match NameE-valueIdentityDescription
AT4G16500.14.3e-0428.72Cystatin/monellin superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000010Cystatin domainPFAMPF16845SQAPIcoord: 3..93
e-value: 5.6E-13
score: 49.0
NoneNo IPR availableGENE3D3.10.450.10coord: 1..97
e-value: 1.8E-14
score: 55.5
NoneNo IPR availableSUPERFAMILY54403Cystatin/monellincoord: 7..93

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC01g0690.1MC01g0690.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0004869 cysteine-type endopeptidase inhibitor activity