MC00g_new0370 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC00g_new0370
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	mitotic spindle checkpoint protein MAD1
Location	scaffold163: 567960 .. 570699 (+)
RNA-Seq Expression	MC00g_new0370
Synteny	MC00g_new0370

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATTAGCAATCTCGTGTTTTATCTTTCCTTCCAGTTAGGACATGGTGATTTTTCCGCAACAAGTACAAAGGTTTTGCGAATGGTAAACACCCTTGCTGTTGACAACGAGGCAAAACAGACTATAGATGCCTTAAAATCTGAATTGCAGAAGACAAAGGAGAAGTTACAAGCTGTTGAAGAATTAAAGACTCCATCAGGTATGAGAACCTTTTCTAATTTATGTTAGTTGAGACTCCATCAGGTATGAGAAACTTTTCTATGAGAGGTTTTTAATTTATGTTTGTTAGTTATTAAGCTGGTCCTGTATAGGTACTCATTTATTTGAGAACCTTGATTATCACATCTTTCCCCCCGGGCTCATTTTCACACTCATTGTAGTGTAGGTCGCATCCTGCTTGCCAAATAAGGTGTTCTCTCTGCAAATCTGAATGGGAATATCTTGAATGTGAGAGAATAAAAATATTCTGTCTCATTATTGAATGTATGAGTTATGTCTTTTAGAAGAAAACCATTACAAATAAGGGAAATAATAAAAATAAAGATAACGTAATTATTACCGTTGTTTTGACTACTAATATTTAGAGATTATATTTACAACAATTATGGAACCCAACAAAATATTTCTTTTTTGTGTAGCTTTTGTTAAATTATATGCGTTACCTTCTTAATTTCCTATTGTAGTTAGGTTTTAATAAATTAGGGTTTCCTTATTCTAGGTAAGTATTTCCTTATCCTAATTGGCAAATTTTAGTTTTCTTTTCTTATTTGTAACTACTATTTATACCCTTATCAACTTTGGAAAGTGACAAGAAAAGAATTATTTTTCTTTATCAATTAACATGGTATCAGAGCCTTGAGGCTTTTTCGATTGGCCTTCATTGCCGATCACTTTTTCAATCGTCCTTAATTGTTGATCAGTTTCCTACTAGTTTTGGGTTTGCCGTTCGTGGTCGATTCGTGGGTTGTGTGGCTGCTGTGTTTTTAGGGCTTCAATAAGATAAGAAAAACTTTTTTTTTTGTCTGGTTCAGATACGGTCTACGGGATTTTCGGTTCGGTCTGATTTGATTTTTCTGTCTGTGCGGCTCAAAGTATTTCCATCCCGGGTCAGCGTATCTCCTGTCCGGTTCAGCGTGTTTCCTACCCGGTTCAACATACACTCAACTCGGTCTAGCCCATTTCGGACCAATTCGGTCTATTTTGGACCCGGTTCAATTTGTTGTCGGTCTGGTTCAGCATTTTATTGCTCGGTTCAGCCCGTTTTTCAGTTCGGTTTGACTGGTTTTGGTTTGGTTCAGCCGGTTTTTGGTTTAGTTCAGCCCTCTTTCGGTTCGGTTCAGTTCAGTGCTGATTTTTCACCCTTCTATTTGGTACTTCAAACTTCAGTTTTATGCATGGATCTCCATTTTTTGAGTTGTTGTTTAGTCTGTTCTAGCACTAATGTCGCAATCATCCAAAATTACCAAAAAATTGGACATACATGAGATCAAACAATTAAAAAGCTTCTTAAAAACTTTGGATAATGCTCCAAGTCAAGGAACAAGATCGATAACGTTGACAAGTACAACTTTTTAGCCTAAACGTTGTACCTGGATTATTGATTCTGGTGCAACTGACCATATTTTTTATGTTTCCCAATTACCCAGAATACTGGGAGTGGAAAATTGAATTTAGTGGGCTAGAATGTGTTGGATATTGAACGTGGCATGTCTATTTCATCAAGTCTAGCCCCATTTTTTTCTTAACAGTATAGATGGAGCCTTAAACTAAATGACTGTAAAATTACAACAAAGGAGAAGGACAAAAAAGTAATAAACTCCTGGATTTAAGATATTTTCTAATATAGATGTTGGAACATTTGATATCGTGTTTAAAAAATCTACTACGTGCTAATATTTAGATGTTAAAAAATTCAATGATTAATCTGGAAAATTTTCTTTGTTGAAACAAAAACTGTTCCAATTGTAACGATGAGTTAAAACAGCTAATGAAACGTACAGTTAAAACAATAAGCAAAAGAGACAGCACAACAAAGAAGCGACACAATGATCATTCATTACTCATTATACAACCAACACATTTTTCTCACTTTTGTGTATTCGCTTGTTAATTATTTAGGTGACGCTGGGAAGGTTGTGGATTCATACATATCTGGAAAAATAATGCAGCTAAAGGAACAAATTGCAACACTTGAAAAGCGTGAAGAGAGGTCAGTGCTTGATGCAATATCCAATAGTTTCAAGATTTGATGAACTCCGGTTTTCTTACTCCTTAAATTAATGGAGCATGATAAGATACTACTGCTATACAGATATAAGACTGTTTTTGCGGATAGAATTTCAGTGTTTAGAAGGGCTTGTTGTGAACTTTTTGGCTACAAGGTATTACACTCTTCCGGAACTATTTTCATCTTCATATTTTGTTGCTTTGCTCCTATATCATTTTCGGATTTGTAATGGAGTATCTTAAAATGAGTTTACTAGGACTTGCAAGTTGCAACTGAGATACCGAGTGAATGTAAAATTGATATTCGAGTTTGACTATAATGTGTTTGTAACTTACTGTATTCTTCTACTCACATTGTTAGTAATTTTGTCAGATTGTTATGGATGAACATCAGCGAGCTGATGGTATTCCTGTTACAAGGTTTACCCTTCAATCAATATATGCTCAAAGTGATGAGGAGAAGCTGCAATTTGAGTATGAATCTGGGAATACAAACATCTTGGTACGATATTAA

mRNA sequence

ATGATTAGCAATCTCGTGTTTTATCTTTCCTTCCAGTTAGGACATGGTGATTTTTCCGCAACAAGTACAAAGGTTTTGCGAATGGTAAACACCCTTGCTGTTGACAACGAGGCAAAACAGACTATAGATGCCTTAAAATCTGAATTGCAGAAGACAAAGGAGAAGTTACAAGCTGTTGAAGAATTAAAGACTCCATCAGGTGACGCTGGGAAGGTTGTGGATTCATACATATCTGGAAAAATAATGCAGCTAAAGGAACAAATTGCAACACTTGAAAAGCGTGAAGAGAGATATAAGACTGTTTTTGCGGATAGAATTTCAGTGTTTAGAAGGGCTTGTTGTGAACTTTTTGGCTACAAGATTGTTATGGATGAACATCAGCGAGCTGATGGTATTCCTGTTACAAGGTTTACCCTTCAATCAATATATGCTCAAAGTGATGAGGAGAAGCTGCAATTTGAGTATGAATCTGGGAATACAAACATCTTGGTACGATATTAA

Coding sequence (CDS)

ATGATTAGCAATCTCGTGTTTTATCTTTCCTTCCAGTTAGGACATGGTGATTTTTCCGCAACAAGTACAAAGGTTTTGCGAATGGTAAACACCCTTGCTGTTGACAACGAGGCAAAACAGACTATAGATGCCTTAAAATCTGAATTGCAGAAGACAAAGGAGAAGTTACAAGCTGTTGAAGAATTAAAGACTCCATCAGGTGACGCTGGGAAGGTTGTGGATTCATACATATCTGGAAAAATAATGCAGCTAAAGGAACAAATTGCAACACTTGAAAAGCGTGAAGAGAGATATAAGACTGTTTTTGCGGATAGAATTTCAGTGTTTAGAAGGGCTTGTTGTGAACTTTTTGGCTACAAGATTGTTATGGATGAACATCAGCGAGCTGATGGTATTCCTGTTACAAGGTTTACCCTTCAATCAATATATGCTCAAAGTGATGAGGAGAAGCTGCAATTTGAGTATGAATCTGGGAATACAAACATCTTGGTACGATATTAA

Protein sequence

MISNLVFYLSFQLGHGDFSATSTKVLRMVNTLAVDNEAKQTIDALKSELQKTKEKLQAVEELKTPSGDAGKVVDSYISGKIMQLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQRADGIPVTRFTLQSIYAQSDEEKLQFEYESGNTNILVRY

Homology

[Close]

BLAST of MC00g_new0370 vs. ExPASy Swiss-Prot
Match: Q9LTY1 (Mitotic spindle checkpoint protein MAD1 OS=Arabidopsis thaliana OX=3702 GN=MAD1 PE=1 SV=1)

HSP 1 Score: 258.8 bits (660), Expect = 4.1e-68
Identity = 130/155 (83.87%), Postives = 147/155 (94.84%), Query Frame = 0


Query: 9   LSFQLGHGDFSATSTKVLRMVNTLAVDNEAKQTIDALKSELQKTKEKLQAVEELKTPSGD 68
           L  +LGHGD+SA +T+VLRMVNTL V+NEAKQTI+AL++ELQKTKE+LQAVEELK+ SGD
Sbjct: 530 LESKLGHGDYSAANTRVLRMVNTLGVENEAKQTIEALQAELQKTKERLQAVEELKSQSGD 589

Query: 69  AGKVVDSYISGKIMQLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQR 128
           AGK+VDS+I+GKI QLKEQ ATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQR
Sbjct: 590 AGKLVDSHITGKIAQLKEQNATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQR 649

Query: 129 ADGIPVTRFTLQSIYAQSDEEKLQFEYESGNTNIL 164
            +GIPVTRFTLQSIYAQSD+EKL+FEYESGNT+IL
Sbjct: 650 PNGIPVTRFTLQSIYAQSDDEKLEFEYESGNTSIL 684

[Close]

BLAST of MC00g_new0370 vs. ExPASy Swiss-Prot
Match: Q9Y6D9 (Mitotic spindle assembly checkpoint protein MAD1 OS=Homo sapiens OX=9606 GN=MAD1L1 PE=1 SV=2)

HSP 1 Score: 54.7 bits (130), Expect = 1.2e-06
Identity = 44/145 (30.34%), Postives = 74/145 (51.03%), Query Frame = 0


Query: 16  GDFSATSTKVLRM-VNTLAVDNE-AKQTIDALKSELQKTKEKLQAVEELKTPSGDAGKVV 75
           GD+  + TKVL M +N  +V  +  ++    L++E ++ +  L+A+E   T   D     
Sbjct: 533 GDYDQSRTKVLHMSLNPTSVARQRLREDHSQLQAECERLRGLLRAMERGGTVPADLEAAA 592

Query: 76  DSYISGK-IMQLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQRADGI 135
            S  S K + +LK+Q+ + E + +R K VF  +I  FR+AC  L GY+I        D  
Sbjct: 593 ASLPSSKEVAELKKQVESAELKNQRLKEVFQTKIQEFRKACYTLTGYQI--------DIT 652

Query: 136 PVTRFTLQSIYAQSDEEKLQFEYES 158
              ++ L S+YA+   + L F+  S
Sbjct: 653 TENQYRLTSLYAEHPGDCLIFKATS 669

[Close]

BLAST of MC00g_new0370 vs. ExPASy Swiss-Prot
Match: Q9WTX8 (Mitotic spindle assembly checkpoint protein MAD1 OS=Mus musculus OX=10090 GN=Mad1l1 PE=1 SV=1)

HSP 1 Score: 48.9 bits (115), Expect = 6.4e-05
Identity = 37/141 (26.24%), Postives = 71/141 (50.35%), Query Frame = 0


Query: 16  GDFSATSTKVLRM-VNTLAVDNEAK-QTIDALKSELQKTKEKLQAVEELKTPSGDAGKVV 75
           GD++ + TKVL M +N +++  + + +  D L+ E ++ +  + A+E       D     
Sbjct: 533 GDYNQSRTKVLHMSLNPISMARQRQHEDHDRLQEECERLRGLVHALERGGPIPADLEAAS 592

Query: 76  DSYISGKIMQLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQRADGIP 135
               S ++ +L++Q+ + E + +R K VF  +I  FR+ C  L GY+I        D   
Sbjct: 593 SLPSSKEVAELRKQVESAELKNQRLKEVFQTKIQEFRKVCYTLTGYQI--------DVTT 652

Query: 136 VTRFTLQSIYAQSDEEKLQFE 155
            +++ L S YA+   + L F+
Sbjct: 653 ESQYRLTSRYAEHQTDCLIFK 665

[Close]

BLAST of MC00g_new0370 vs. ExPASy Swiss-Prot
Match: Q80YF0 (Mitotic spindle assembly checkpoint protein MAD1 OS=Cricetulus griseus OX=10029 GN=MAD1L1 PE=2 SV=1)

HSP 1 Score: 47.4 bits (111), Expect = 1.9e-04
Identity = 38/141 (26.95%), Postives = 69/141 (48.94%), Query Frame = 0


Query: 16  GDFSATSTKVLRM-VNTLAVDNEAKQTIDA-LKSELQKTKEKLQAVEELKTPSGDAGKVV 75
           GD++ + TKVL M +N  ++  + +Q   A L+ E ++ +  + A+E       D     
Sbjct: 533 GDYNQSRTKVLHMSLNPASMARKRQQEDHARLQGECERLRGLVHALERGGPIPADLEVAS 592

Query: 76  DSYISGKIMQLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQRADGIP 135
               S ++ +L++Q+ + E + +R K VF  +I  FR+ C  L GY+I        D   
Sbjct: 593 SLPSSKEVAELRKQVESAELKNQRLKEVFQTKIQEFRKVCYTLTGYQI--------DVTT 652

Query: 136 VTRFTLQSIYAQSDEEKLQFE 155
             ++ L S YA+   + L F+
Sbjct: 653 ENQYRLTSRYAEHQSDCLIFK 665

[Close]

BLAST of MC00g_new0370 vs. NCBI nr
Match: XP_022155150.1 (mitotic spindle checkpoint protein MAD1 isoform X1 [Momordica charantia])

HSP 1 Score: 294 bits (753), Expect = 1.03e-92
Identity = 151/153 (98.69%), Postives = 152/153 (99.35%), Query Frame = 0


Query: 12  QLGHGDFSATSTKVLRMVNTLAVDNEAKQTIDALKSELQKTKEKLQAVEELKTPSGDAGK 71
           +LGHGDFSATSTKVLRMVNTLAVDNEAKQTIDALKSELQKTKEKLQAVEELKTPSGDAGK
Sbjct: 534 KLGHGDFSATSTKVLRMVNTLAVDNEAKQTIDALKSELQKTKEKLQAVEELKTPSGDAGK 593

Query: 72  VVDSYISGKIMQLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQRADG 131
           VVDSYISGKIMQLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQRADG
Sbjct: 594 VVDSYISGKIMQLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQRADG 653

Query: 132 IPVTRFTLQSIYAQSDEEKLQFEYESGNTNILV 164
           IPVTRFTLQSIYAQSDEEKLQFEYESGNTNIL 
Sbjct: 654 IPVTRFTLQSIYAQSDEEKLQFEYESGNTNILA 686

[Close]

BLAST of MC00g_new0370 vs. NCBI nr
Match: XP_038876539.1 (mitotic spindle checkpoint protein MAD1 isoform X2 [Benincasa hispida])

HSP 1 Score: 285 bits (728), Expect = 1.83e-90
Identity = 144/153 (94.12%), Postives = 151/153 (98.69%), Query Frame = 0


Query: 12  QLGHGDFSATSTKVLRMVNTLAVDNEAKQTIDALKSELQKTKEKLQAVEELKTPSGDAGK 71
           +LGHGDFS+T+TKVLRMVNTLAVDNEAKQTI+ALKSELQKTKEKLQAVEELKTPSGDAGK
Sbjct: 389 KLGHGDFSSTNTKVLRMVNTLAVDNEAKQTIEALKSELQKTKEKLQAVEELKTPSGDAGK 448

Query: 72  VVDSYISGKIMQLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQRADG 131
           +VDSYISGKI+QLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQRADG
Sbjct: 449 LVDSYISGKILQLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQRADG 508

Query: 132 IPVTRFTLQSIYAQSDEEKLQFEYESGNTNILV 164
           IPVTRF LQSIYAQSD+EKLQFEYESGNTNIL 
Sbjct: 509 IPVTRFILQSIYAQSDDEKLQFEYESGNTNILA 541

[Close]

BLAST of MC00g_new0370 vs. NCBI nr
Match: XP_004142360.1 (mitotic spindle checkpoint protein MAD1 isoform X1 [Cucumis sativus] >KAE8648888.1 hypothetical protein Csa_008623 [Cucumis sativus])

HSP 1 Score: 287 bits (734), Expect = 6.75e-90
Identity = 145/153 (94.77%), Postives = 152/153 (99.35%), Query Frame = 0


Query: 12  QLGHGDFSATSTKVLRMVNTLAVDNEAKQTIDALKSELQKTKEKLQAVEELKTPSGDAGK 71
           ++GHGDFS+T+TKVLRMVNTLAVDNEAKQTI+ALKSELQKTKEKLQAVEELK PSGDAGK
Sbjct: 534 KIGHGDFSSTNTKVLRMVNTLAVDNEAKQTIEALKSELQKTKEKLQAVEELKAPSGDAGK 593

Query: 72  VVDSYISGKIMQLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQRADG 131
           +VDSYISGKIMQLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQRADG
Sbjct: 594 LVDSYISGKIMQLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQRADG 653

Query: 132 IPVTRFTLQSIYAQSDEEKLQFEYESGNTNILV 164
           IPVTRFTLQSIYAQSD+EKLQFEYESGNTNILV
Sbjct: 654 IPVTRFTLQSIYAQSDDEKLQFEYESGNTNILV 686

[Close]

BLAST of MC00g_new0370 vs. NCBI nr
Match: KAG6601670.1 (Mitotic spindle checkpoint protein MAD1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7032439.1 Mitotic spindle checkpoint protein MAD1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 286 bits (733), Expect = 9.49e-90
Identity = 145/153 (94.77%), Postives = 151/153 (98.69%), Query Frame = 0


Query: 12  QLGHGDFSATSTKVLRMVNTLAVDNEAKQTIDALKSELQKTKEKLQAVEELKTPSGDAGK 71
           +LGHGDFS+T+TKVLRMVNTLAVDNEAKQTI+ALKSELQKTKEKLQAVEELKTPSGDAGK
Sbjct: 534 KLGHGDFSSTNTKVLRMVNTLAVDNEAKQTIEALKSELQKTKEKLQAVEELKTPSGDAGK 593

Query: 72  VVDSYISGKIMQLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQRADG 131
           +VDSYISGKIMQLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQR DG
Sbjct: 594 LVDSYISGKIMQLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQRTDG 653

Query: 132 IPVTRFTLQSIYAQSDEEKLQFEYESGNTNILV 164
           IPVTRFTLQSIYAQSD+EKLQFEYESGNTNIL 
Sbjct: 654 IPVTRFTLQSIYAQSDDEKLQFEYESGNTNILA 686

[Close]

BLAST of MC00g_new0370 vs. NCBI nr
Match: XP_022997454.1 (mitotic spindle checkpoint protein MAD1 [Cucurbita maxima])

HSP 1 Score: 286 bits (733), Expect = 9.49e-90
Identity = 145/153 (94.77%), Postives = 151/153 (98.69%), Query Frame = 0


Query: 12  QLGHGDFSATSTKVLRMVNTLAVDNEAKQTIDALKSELQKTKEKLQAVEELKTPSGDAGK 71
           +LGHGDFS+T+TKVLRMVNTLAVDNEAKQTI+ALKSELQKTKEKLQAVEELKTPSGDAGK
Sbjct: 534 KLGHGDFSSTNTKVLRMVNTLAVDNEAKQTIEALKSELQKTKEKLQAVEELKTPSGDAGK 593

Query: 72  VVDSYISGKIMQLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQRADG 131
           +VDSYISGKIMQLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQR DG
Sbjct: 594 LVDSYISGKIMQLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQRTDG 653

Query: 132 IPVTRFTLQSIYAQSDEEKLQFEYESGNTNILV 164
           IPVTRFTLQSIYAQSD+EKLQFEYESGNTNIL 
Sbjct: 654 IPVTRFTLQSIYAQSDDEKLQFEYESGNTNILA 686

[Close]

BLAST of MC00g_new0370 vs. ExPASy TrEMBL
Match: A0A6J1DLL9 (mitotic spindle checkpoint protein MAD1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111022291 PE=3 SV=1)

HSP 1 Score: 294 bits (753), Expect = 4.99e-93
Identity = 151/153 (98.69%), Postives = 152/153 (99.35%), Query Frame = 0


Query: 12  QLGHGDFSATSTKVLRMVNTLAVDNEAKQTIDALKSELQKTKEKLQAVEELKTPSGDAGK 71
           +LGHGDFSATSTKVLRMVNTLAVDNEAKQTIDALKSELQKTKEKLQAVEELKTPSGDAGK
Sbjct: 534 KLGHGDFSATSTKVLRMVNTLAVDNEAKQTIDALKSELQKTKEKLQAVEELKTPSGDAGK 593

Query: 72  VVDSYISGKIMQLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQRADG 131
           VVDSYISGKIMQLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQRADG
Sbjct: 594 VVDSYISGKIMQLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQRADG 653

Query: 132 IPVTRFTLQSIYAQSDEEKLQFEYESGNTNILV 164
           IPVTRFTLQSIYAQSDEEKLQFEYESGNTNIL 
Sbjct: 654 IPVTRFTLQSIYAQSDEEKLQFEYESGNTNILA 686

[Close]

BLAST of MC00g_new0370 vs. ExPASy TrEMBL
Match: A0A0A0KV95 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G630750 PE=3 SV=1)

HSP 1 Score: 287 bits (734), Expect = 2.53e-90
Identity = 145/153 (94.77%), Postives = 152/153 (99.35%), Query Frame = 0


Query: 12  QLGHGDFSATSTKVLRMVNTLAVDNEAKQTIDALKSELQKTKEKLQAVEELKTPSGDAGK 71
           ++GHGDFS+T+TKVLRMVNTLAVDNEAKQTI+ALKSELQKTKEKLQAVEELK PSGDAGK
Sbjct: 534 KIGHGDFSSTNTKVLRMVNTLAVDNEAKQTIEALKSELQKTKEKLQAVEELKAPSGDAGK 593

Query: 72  VVDSYISGKIMQLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQRADG 131
           +VDSYISGKIMQLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQRADG
Sbjct: 594 LVDSYISGKIMQLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQRADG 653

Query: 132 IPVTRFTLQSIYAQSDEEKLQFEYESGNTNILV 164
           IPVTRFTLQSIYAQSD+EKLQFEYESGNTNILV
Sbjct: 654 IPVTRFTLQSIYAQSDDEKLQFEYESGNTNILV 686

[Close]

BLAST of MC00g_new0370 vs. ExPASy TrEMBL
Match: A0A6J1KBD3 (mitotic spindle checkpoint protein MAD1 OS=Cucurbita maxima OX=3661 GN=LOC111492363 PE=3 SV=1)

HSP 1 Score: 286 bits (733), Expect = 4.60e-90
Identity = 145/153 (94.77%), Postives = 151/153 (98.69%), Query Frame = 0


Query: 12  QLGHGDFSATSTKVLRMVNTLAVDNEAKQTIDALKSELQKTKEKLQAVEELKTPSGDAGK 71
           +LGHGDFS+T+TKVLRMVNTLAVDNEAKQTI+ALKSELQKTKEKLQAVEELKTPSGDAGK
Sbjct: 534 KLGHGDFSSTNTKVLRMVNTLAVDNEAKQTIEALKSELQKTKEKLQAVEELKTPSGDAGK 593

Query: 72  VVDSYISGKIMQLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQRADG 131
           +VDSYISGKIMQLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQR DG
Sbjct: 594 LVDSYISGKIMQLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQRTDG 653

Query: 132 IPVTRFTLQSIYAQSDEEKLQFEYESGNTNILV 164
           IPVTRFTLQSIYAQSD+EKLQFEYESGNTNIL 
Sbjct: 654 IPVTRFTLQSIYAQSDDEKLQFEYESGNTNILA 686

[Close]

BLAST of MC00g_new0370 vs. ExPASy TrEMBL
Match: A0A6J1EY58 (mitotic spindle checkpoint protein MAD1 OS=Cucurbita moschata OX=3662 GN=LOC111439783 PE=3 SV=1)

HSP 1 Score: 286 bits (733), Expect = 4.60e-90
Identity = 145/153 (94.77%), Postives = 151/153 (98.69%), Query Frame = 0


Query: 12  QLGHGDFSATSTKVLRMVNTLAVDNEAKQTIDALKSELQKTKEKLQAVEELKTPSGDAGK 71
           +LGHGDFS+T+TKVLRMVNTLAVDNEAKQTI+ALKSELQKTKEKLQAVEELKTPSGDAGK
Sbjct: 534 KLGHGDFSSTNTKVLRMVNTLAVDNEAKQTIEALKSELQKTKEKLQAVEELKTPSGDAGK 593

Query: 72  VVDSYISGKIMQLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQRADG 131
           +VDSYISGKIMQLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQR DG
Sbjct: 594 LVDSYISGKIMQLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQRTDG 653

Query: 132 IPVTRFTLQSIYAQSDEEKLQFEYESGNTNILV 164
           IPVTRFTLQSIYAQSD+EKLQFEYESGNTNIL 
Sbjct: 654 IPVTRFTLQSIYAQSDDEKLQFEYESGNTNILA 686

[Close]

BLAST of MC00g_new0370 vs. ExPASy TrEMBL
Match: A0A1S3CCD1 (mitotic spindle checkpoint protein MAD1 OS=Cucumis melo OX=3656 GN=LOC103499375 PE=3 SV=1)

HSP 1 Score: 285 bits (730), Expect = 1.28e-89
Identity = 144/153 (94.12%), Postives = 151/153 (98.69%), Query Frame = 0


Query: 12  QLGHGDFSATSTKVLRMVNTLAVDNEAKQTIDALKSELQKTKEKLQAVEELKTPSGDAGK 71
           ++GHGDFS+T+TKVLRMVNTLAVDNEAKQTI+ALKSELQKTKEKLQAVEELK PSGDAGK
Sbjct: 534 KIGHGDFSSTNTKVLRMVNTLAVDNEAKQTIEALKSELQKTKEKLQAVEELKAPSGDAGK 593

Query: 72  VVDSYISGKIMQLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQRADG 131
           +VDSYISGKIMQLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQRADG
Sbjct: 594 LVDSYISGKIMQLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQRADG 653

Query: 132 IPVTRFTLQSIYAQSDEEKLQFEYESGNTNILV 164
           IPVTRFTLQSIYAQSD+EKLQFEYESGNTNIL 
Sbjct: 654 IPVTRFTLQSIYAQSDDEKLQFEYESGNTNILA 686

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BLAST of MC00g_new0370 vs. TAIR 10
Match: AT5G49880.1 (mitotic checkpoint family protein )

HSP 1 Score: 258.8 bits (660), Expect = 2.9e-69
Identity = 130/155 (83.87%), Postives = 147/155 (94.84%), Query Frame = 0


Query: 9   LSFQLGHGDFSATSTKVLRMVNTLAVDNEAKQTIDALKSELQKTKEKLQAVEELKTPSGD 68
           L  +LGHGD+SA +T+VLRMVNTL V+NEAKQTI+AL++ELQKTKE+LQAVEELK+ SGD
Sbjct: 530 LESKLGHGDYSAANTRVLRMVNTLGVENEAKQTIEALQAELQKTKERLQAVEELKSQSGD 589

Query: 69  AGKVVDSYISGKIMQLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQR 128
           AGK+VDS+I+GKI QLKEQ ATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQR
Sbjct: 590 AGKLVDSHITGKIAQLKEQNATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQR 649

Query: 129 ADGIPVTRFTLQSIYAQSDEEKLQFEYESGNTNIL 164
            +GIPVTRFTLQSIYAQSD+EKL+FEYESGNT+IL
Sbjct: 650 PNGIPVTRFTLQSIYAQSDDEKLEFEYESGNTSIL 684

The following BLAST results are available for this feature:

BLAST of MC00g_new0370 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LTY1	4.1e-68	83.87	Mitotic spindle checkpoint protein MAD1 OS=Arabidopsis thaliana OX=3702 GN=MAD1 ...	[more]
Q9Y6D9	1.2e-06	30.34	Mitotic spindle assembly checkpoint protein MAD1 OS=Homo sapiens OX=9606 GN=MAD1...	[more]
Q9WTX8	6.4e-05	26.24	Mitotic spindle assembly checkpoint protein MAD1 OS=Mus musculus OX=10090 GN=Mad...	[more]
Q80YF0	1.9e-04	26.95	Mitotic spindle assembly checkpoint protein MAD1 OS=Cricetulus griseus OX=10029 ...	[more]

BLAST of MC00g_new0370 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022155150.1	1.03e-92	98.69	mitotic spindle checkpoint protein MAD1 isoform X1 [Momordica charantia]	[more]
XP_038876539.1	1.83e-90	94.12	mitotic spindle checkpoint protein MAD1 isoform X2 [Benincasa hispida]	[more]
XP_004142360.1	6.75e-90	94.77	mitotic spindle checkpoint protein MAD1 isoform X1 [Cucumis sativus] >KAE8648888...	[more]
KAG6601670.1	9.49e-90	94.77	Mitotic spindle checkpoint protein MAD1, partial [Cucurbita argyrosperma subsp. ...	[more]
XP_022997454.1	9.49e-90	94.77	mitotic spindle checkpoint protein MAD1 [Cucurbita maxima]	[more]

BLAST of MC00g_new0370 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DLL9	4.99e-93	98.69	mitotic spindle checkpoint protein MAD1 isoform X1 OS=Momordica charantia OX=367...	[more]
A0A0A0KV95	2.53e-90	94.77	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G630750 PE=3 SV=1	[more]
A0A6J1KBD3	4.60e-90	94.77	mitotic spindle checkpoint protein MAD1 OS=Cucurbita maxima OX=3661 GN=LOC111492...	[more]
A0A6J1EY58	4.60e-90	94.77	mitotic spindle checkpoint protein MAD1 OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A1S3CCD1	1.28e-89	94.12	mitotic spindle checkpoint protein MAD1 OS=Cucumis melo OX=3656 GN=LOC103499375 ...	[more]

BLAST of MC00g_new0370 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G49880.1	2.9e-69	83.87	mitotic checkpoint family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 81..101
None	No IPR available	COILS	Coil	Coil	coord: 35..62
None	No IPR available	GENE3D	1.20.5.170		coord: 78..116 e-value: 3.8E-6 score: 28.6
None	No IPR available	GENE3D	3.30.457.60		coord: 118..165 e-value: 5.8E-7 score: 31.7
None	No IPR available	SUPERFAMILY	75704	Mitotic arrest deficient-like 1, Mad1	coord: 11..61
IPR008672	Spindle assembly checkpoint component Mad1	PFAM	PF05557	MAD	coord: 15..159 e-value: 4.3E-14 score: 51.8
IPR008672	Spindle assembly checkpoint component Mad1	PANTHER	PTHR23168	MITOTIC SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD1 MITOTIC ARREST DEFICIENT-LIKE PROTEIN 1	coord: 8..164

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC00g_new0370.1	MC00g_new0370.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0007094	mitotic spindle assembly checkpoint signaling
cellular_component	GO:0005634	nucleus