MC00g0473 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC00g0473
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Location	scaffold196: 158005 .. 160701 (+)
RNA-Seq Expression	MC00g0473
Synteny	MC00g0473

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

CCCTACGCCATAAAATGGCCCACTCATGGCCCCTCCCTCCCTCCCCAATTTCCACACATAATATATATATATTTTACAAACCAAATTCACAAATACCGCCGCCGTCCGCCGTCGACCGTCCTCTTCCATGGCTCTCCTCCGCACGAAGAGTCGGTTGACGATTCCGGCACCGCCGCCGTCGCCGATCCCGACCGGCACCGGATCGCGCTCGGCGGCCAACGAGACCTTCAAGGAATTCCTCGAGACGAAGTCGATTCAACTCCCGCAACTCTCGCTGCCGGAATCTCGCTTCGTCTCCGGCGCCAATCCCTTGCCCGCGCTCCTCGATTACCGATTGCTCGCGTCTCCCGACGGCGACGCGGTGGCGCGGATGCTCCGTTCGGCCGGCGAGTTCGGCGCCTTTCGGATCGTTAATCACGGAATTTCCGGGGAGGAAATTTTGTCGGTGGTGAAGGACGCGAAATCTATTCTGGAAGATTCTTCTTCGGAGAGGAATGACGGAGCCCGGGCGGCGATTGTACAGGTCCGCCGTCGGAGACACGGCGGGGCGTCGGAACATTCGGTTGCGCGGGACGAGGCGTACCGGCACTTTAGGTAAACCCGAAACAACCCGATAACTTCGCGAGTTTCCGATTAGTTAGAAAATGGGCTGGGCTGAGCTGGACTTGGCTGTTTCTGGCCCAAATTAGGCCCACTTTCTATAGAAATAAGAGTATTTATTCAAACAAAAATAATATTTCATTAGATTAGTACTTAGTAGCGTAATTGAGTTGGTTTTCAGTGAATAAATTTACTTCAATTAATGAGAAAAATAAGTTTACTATTATATAATTATAGTTTATTTTTAATTAGTAAGTTCTTTTTTAAAACTTTCTAATTCTAAATCTTCACTTAATATCATTTTGATATAGTTTATTTCTCATATTTAAAAAAAAAAATTAACCTGTTGTGTTCAATATTTCTCTGAACTTTAAAAATTGTTAAGTAGATATGGTTTAAACTTTTCATCTTTTTAAGCATATTAAAAAAATTTAAAATTCAAAATACTTGTCCTAAAACAAAATTGAAAATATTTAAAGTATAGAAGTTTATTAAACTCAAAAATTAAAACCCATCGGATAACTATTTTAATTTTAGTTTCTATTTTTAAAAATTAAGCTTACAAACATTATTGTCAGTAGTGTGTTCCTTTGTTTTGTTTTGTTGCGTAGATTTTATAAATCTCCAAAAAATCTTCAAATATGATCGTACGTCATGGATATCAATGACAACTAATATTTTGACTATTTTTTCAACTGGGTTCGACTCGGCTCGGCTCGATTTGCAGCGGAGAGATGGAGAAGGTAGCCAGGAAAGTAGAGGGAATTGGAGAGAAATTGAGTGAGATTTTATCAGAAAGCATGGGGGAGGAATGGGGCGAAGATCAGAAGGTGAAGAAGAAGAGAGGAGAAAAAGAGGCAATTTTGAGCATTTTCAGATACAATAATAATCAGCAGAATCAATTTGATGATGGAGGAGAGAGAGAAAATGAAGAGAGAGAAAGTGATGAGAGTGTGATGATGAGCCTCCACATTCCAGCAGAGCACTGCCAATTCTCTGTCAATCCTCACACACAGGGCTCTTTCTGCTTTGATTCTGCTGCTGATACCATTGTTGTCACCATTGGCAAACAGCTCCAGGTAATTTTATCAATATCCATCCATTTCCTAATGATTTGTTCTTTTATAAAAAAATGTAAATTCCATAAGGTGTATCAATTTAGAAGTGCTACAATTTAGGTCTAGGATTCAAATTTGGATGAATATTTTGGTCCCTTCTCGAAAATAGGTTAAAAAATGCATTTGGTCAATGTACTTTTATTAAAGTAACGATTTAATCTCTAAACTTTTAATTGTAACGTTTTAGTTCCTATAGTTTCATATTGATAACAATTTAGTCCTTGTAACAATTTAATCTCTGTCACAAAAAACTCTCATCAAAATTAAGCACTAAATTGTTACGAACTTTAAACCTCAGGGACAAAACTATTACATAGTTAGATTTCAAGGACTAAATTATTACCAATTTGAAACTACATGAACTAAATTGTTACTTTTGGAAGTTGATGGACTAAATCATTACTCCAATTGAAGTACAGGGATAAAAAGTGTTTTTTAACCTTAAAAATACAATGAATAAAATGGACACTTCGAAAGTATAGATACTAAAATGTAAAAAAAAAAAAAAAAATGGAAGAAAATTTGCATAGATTTTTGAAGAAAAAATGGTTTGATAATGATTTGTGATGGTGGTGTAGGAATGGAGCATGGGAAAATTAAAAAGTGCAAGAAGTAAGATGATATTTGTACCAAATGGAAGCCAAAGCCCTTTCTCCATTGAGCTCAAAATTTCACACCCAAAATTACTCCATAATCCCCATTCCAATATCATCTCAATTTCTGACCAAATCTTCATTGCCCTTCTTCTTTTTTCCCTTTACCTTCTTTATACTTACACATCTTCATTATTTAAGGCCAAATGATCCTTTTAAATGAACCAAGGAAAAAAAAAAAAGAAAAAGGAATTGCCTCTCTTGTTTTTGCACCATTTGTGATTGATATTTTTATGCCAAGGTTTTTGTTTTTGGTATAGAATGATTGGTTTGTATAGAGATTGGTGTGACATTTGGCTTGCAAAAGTTGAAGGGTTATTATGGTA

mRNA sequence

CCCTACGCCATAAAATGGCCCACTCATGGCCCCTCCCTCCCTCCCCAATTTCCACACATAATATATATATATTTTACAAACCAAATTCACAAATACCGCCGCCGTCCGCCGTCGACCGTCCTCTTCCATGGCTCTCCTCCGCACGAAGAGTCGGTTGACGATTCCGGCACCGCCGCCGTCGCCGATCCCGACCGGCACCGGATCGCGCTCGGCGGCCAACGAGACCTTCAAGGAATTCCTCGAGACGAAGTCGATTCAACTCCCGCAACTCTCGCTGCCGGAATCTCGCTTCGTCTCCGGCGCCAATCCCTTGCCCGCGCTCCTCGATTACCGATTGCTCGCGTCTCCCGACGGCGACGCGGTGGCGCGGATGCTCCGTTCGGCCGGCGAGTTCGGCGCCTTTCGGATCGTTAATCACGGAATTTCCGGGGAGGAAATTTTGTCGGTGGTGAAGGACGCGAAATCTATTCTGGAAGATTCTTCTTCGGAGAGGAATGACGGAGCCCGGGCGGCGATTGTACAGGTCCGCCGTCGGAGACACGGCGGGGCGTCGGAACATTCGGTTGCGCGGGACGAGGCGTACCGGCACTTTAGCGGAGAGATGGAGAAGGTAGCCAGGAAAGTAGAGGGAATTGGAGAGAAATTGAGTGAGATTTTATCAGAAAGCATGGGGGAGGAATGGGGCGAAGATCAGAAGGTGAAGAAGAAGAGAGGAGAAAAAGAGGCAATTTTGAGCATTTTCAGATACAATAATAATCAGCAGAATCAATTTGATGATGGAGGAGAGAGAGAAAATGAAGAGAGAGAAAGTGATGAGAGTGTGATGATGAGCCTCCACATTCCAGCAGAGCACTGCCAATTCTCTGTCAATCCTCACACACAGGGCTCTTTCTGCTTTGATTCTGCTGCTGATACCATTGTTGTCACCATTGGCAAACAGCTCCAGGAATGGAGCATGGGAAAATTAAAAAGTGCAAGAAGTAAGATGATATTTGTACCAAATGGAAGCCAAAGCCCTTTCTCCATTGAGCTCAAAATTTCACACCCAAAATTACTCCATAATCCCCATTCCAATATCATCTCAATTTCTGACCAAATCTTCATTGCCCTTCTTCTTTTTTCCCTTTACCTTCTTTATACTTACACATCTTCATTATTTAAGGCCAAATGATCCTTTTAAATGAACCAAGGAAAAAAAAAAAAGAAAAAGGAATTGCCTCTCTTGTTTTTGCACCATTTGTGATTGATATTTTTATGCCAAGGTTTTTGTTTTTGGTATAGAATGATTGGTTTGTATAGAGATTGGTGTGACATTTGGCTTGCAAAAGTTGAAGGGTTATTATGGTA

Coding sequence (CDS)

ATGGCTCTCCTCCGCACGAAGAGTCGGTTGACGATTCCGGCACCGCCGCCGTCGCCGATCCCGACCGGCACCGGATCGCGCTCGGCGGCCAACGAGACCTTCAAGGAATTCCTCGAGACGAAGTCGATTCAACTCCCGCAACTCTCGCTGCCGGAATCTCGCTTCGTCTCCGGCGCCAATCCCTTGCCCGCGCTCCTCGATTACCGATTGCTCGCGTCTCCCGACGGCGACGCGGTGGCGCGGATGCTCCGTTCGGCCGGCGAGTTCGGCGCCTTTCGGATCGTTAATCACGGAATTTCCGGGGAGGAAATTTTGTCGGTGGTGAAGGACGCGAAATCTATTCTGGAAGATTCTTCTTCGGAGAGGAATGACGGAGCCCGGGCGGCGATTGTACAGGTCCGCCGTCGGAGACACGGCGGGGCGTCGGAACATTCGGTTGCGCGGGACGAGGCGTACCGGCACTTTAGCGGAGAGATGGAGAAGGTAGCCAGGAAAGTAGAGGGAATTGGAGAGAAATTGAGTGAGATTTTATCAGAAAGCATGGGGGAGGAATGGGGCGAAGATCAGAAGGTGAAGAAGAAGAGAGGAGAAAAAGAGGCAATTTTGAGCATTTTCAGATACAATAATAATCAGCAGAATCAATTTGATGATGGAGGAGAGAGAGAAAATGAAGAGAGAGAAAGTGATGAGAGTGTGATGATGAGCCTCCACATTCCAGCAGAGCACTGCCAATTCTCTGTCAATCCTCACACACAGGGCTCTTTCTGCTTTGATTCTGCTGCTGATACCATTGTTGTCACCATTGGCAAACAGCTCCAGGAATGGAGCATGGGAAAATTAAAAAGTGCAAGAAGTAAGATGATATTTGTACCAAATGGAAGCCAAAGCCCTTTCTCCATTGAGCTCAAAATTTCACACCCAAAATTACTCCATAATCCCCATTCCAATATCATCTCAATTTCTGACCAAATCTTCATTGCCCTTCTTCTTTTTTCCCTTTACCTTCTTTATACTTACACATCTTCATTATTTAAGGCCAAATGA

Protein sequence

MALLRTKSRLTIPAPPPSPIPTGTGSRSAANETFKEFLETKSIQLPQLSLPESRFVSGANPLPALLDYRLLASPDGDAVARMLRSAGEFGAFRIVNHGISGEEILSVVKDAKSILEDSSSERNDGARAAIVQVRRRRHGGASEHSVARDEAYRHFSGEMEKVARKVEGIGEKLSEILSESMGEEWGEDQKVKKKRGEKEAILSIFRYNNNQQNQFDDGGERENEERESDESVMMSLHIPAEHCQFSVNPHTQGSFCFDSAADTIVVTIGKQLQEWSMGKLKSARSKMIFVPNGSQSPFSIELKISHPKLLHNPHSNIISISDQIFIALLLFSLYLLYTYTSSLFKAK

Homology

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BLAST of MC00g0473 vs. NCBI nr
Match: XP_022144007.1 (uncharacterized protein LOC111013795 [Momordica charantia])

HSP 1 Score: 662 bits (1709), Expect = 1.69e-239
Identity = 347/347 (100.00%), Postives = 347/347 (100.00%), Query Frame = 0


Query: 1   MALLRTKSRLTIPAPPPSPIPTGTGSRSAANETFKEFLETKSIQLPQLSLPESRFVSGAN 60
           MALLRTKSRLTIPAPPPSPIPTGTGSRSAANETFKEFLETKSIQLPQLSLPESRFVSGAN
Sbjct: 1   MALLRTKSRLTIPAPPPSPIPTGTGSRSAANETFKEFLETKSIQLPQLSLPESRFVSGAN 60

Query: 61  PLPALLDYRLLASPDGDAVARMLRSAGEFGAFRIVNHGISGEEILSVVKDAKSILEDSSS 120
           PLPALLDYRLLASPDGDAVARMLRSAGEFGAFRIVNHGISGEEILSVVKDAKSILEDSSS
Sbjct: 61  PLPALLDYRLLASPDGDAVARMLRSAGEFGAFRIVNHGISGEEILSVVKDAKSILEDSSS 120

Query: 121 ERNDGARAAIVQVRRRRHGGASEHSVARDEAYRHFSGEMEKVARKVEGIGEKLSEILSES 180
           ERNDGARAAIVQVRRRRHGGASEHSVARDEAYRHFSGEMEKVARKVEGIGEKLSEILSES
Sbjct: 121 ERNDGARAAIVQVRRRRHGGASEHSVARDEAYRHFSGEMEKVARKVEGIGEKLSEILSES 180

Query: 181 MGEEWGEDQKVKKKRGEKEAILSIFRYNNNQQNQFDDGGERENEERESDESVMMSLHIPA 240
           MGEEWGEDQKVKKKRGEKEAILSIFRYNNNQQNQFDDGGERENEERESDESVMMSLHIPA
Sbjct: 181 MGEEWGEDQKVKKKRGEKEAILSIFRYNNNQQNQFDDGGERENEERESDESVMMSLHIPA 240

Query: 241 EHCQFSVNPHTQGSFCFDSAADTIVVTIGKQLQEWSMGKLKSARSKMIFVPNGSQSPFSI 300
           EHCQFSVNPHTQGSFCFDSAADTIVVTIGKQLQEWSMGKLKSARSKMIFVPNGSQSPFSI
Sbjct: 241 EHCQFSVNPHTQGSFCFDSAADTIVVTIGKQLQEWSMGKLKSARSKMIFVPNGSQSPFSI 300

Query: 301 ELKISHPKLLHNPHSNIISISDQIFIALLLFSLYLLYTYTSSLFKAK 347
           ELKISHPKLLHNPHSNIISISDQIFIALLLFSLYLLYTYTSSLFKAK
Sbjct: 301 ELKISHPKLLHNPHSNIISISDQIFIALLLFSLYLLYTYTSSLFKAK 347

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BLAST of MC00g0473 vs. NCBI nr
Match: XP_038881407.1 (uncharacterized protein LOC120072944, partial [Benincasa hispida])

HSP 1 Score: 383 bits (984), Expect = 3.91e-129
Identity = 228/358 (63.69%), Postives = 264/358 (73.74%), Query Frame = 0


Query: 10  LTIPAPPPSPIPTGTGSRSAANETFKEFLETKSIQLPQLSLPESRFVSGANPLPALLDYR 69
           + IPAPPPSPIPTGTGSRSAANETFK FLE KSI LPQLSLPESRF+SG NP PA++D+R
Sbjct: 1   IIIPAPPPSPIPTGTGSRSAANETFKTFLE-KSIHLPQLSLPESRFLSGVNPTPAVVDFR 60

Query: 70  LLASPDG-DAVARMLRSAGEFGAFRIVNHGISGEEILSVVKDAKSILEDSSSE------- 129
            L SP G +A ARMLRS  EFGAFRIVNHGISGEEILSVV +AKS+LED +         
Sbjct: 61  SLVSPGGGEAAARMLRSVNEFGAFRIVNHGISGEEILSVVNEAKSVLEDCNKGVDDRGWF 120

Query: 130 RNDGARAAIVQVRRRRHGGASEHSVARDEAYRHFSGEMEKVARKVEGIGEKLSEILSESM 189
            NDG R AI+Q+RRR    ASE+++   E  R  SG+ME++  K+EGI EKLSEIL + M
Sbjct: 121 ENDGNREAILQLRRRNDSKASENTIVPAETNRQISGKMERIRSKLEGIAEKLSEILWKCM 180

Query: 190 GEEWGEDQKVKKKRGEKEAILSIFRYNNNQQ------NQFDDGGERENEERESDESVMMS 249
           GE         KKR +KEAI SI+RYNNNQ       N  ++  + + EERE+D + MM 
Sbjct: 181 GENV-------KKRDKKEAIFSIYRYNNNQNILERENNNNNNIAKNDKEERENDGNEMMR 240

Query: 250 LHIPAEHCQFSVNPHTQG--SFCFDSAADTIVVTIGKQLQEWSMGKLKSARSKMIFVPN- 309
           LHIP EHCQF VN H Q   SFCFD+AADTIVVTIGKQLQE S+GKLKSARS+MIF+ + 
Sbjct: 241 LHIPGEHCQFYVNSHQQEQPSFCFDAAADTIVVTIGKQLQELSLGKLKSARSEMIFLTDL 300

Query: 310 -GSQSPFSIELKISHPKLL--HNPHSNIISISDQIFIALLLFSLYLLYTYTSSLFKAK 347
            GS++ FSIELKIS+P LL  HN HS IISISDQIFIA L  SLY LYT+ SS FK K
Sbjct: 301 LGSKASFSIELKISNPNLLKNHNSHSKIISISDQIFIAFLFLSLYFLYTHISSFFKGK 350

[Close]

BLAST of MC00g0473 vs. NCBI nr
Match: XP_008463252.1 (PREDICTED: uncharacterized protein LOC103501456 [Cucumis melo])

HSP 1 Score: 367 bits (941), Expect = 2.82e-122
Identity = 225/368 (61.14%), Postives = 262/368 (71.20%), Query Frame = 0


Query: 1   MALLRTKSRLTIPAPPPSPIPTGTGSRSAANETFKEFLETKSIQLPQLSLPESRFVSGAN 60
           MALLRTKSRLTIPAPPPSPIPT TGSRSA NETFK FLE  S  LPQLSLPESRF SG N
Sbjct: 12  MALLRTKSRLTIPAPPPSPIPTATGSRSAGNETFKTFLEN-STHLPQLSLPESRFASGFN 71

Query: 61  PLPALLDYR-LLASPDGDAVARMLRSAGEFGAFRIVNHGISGEEILSVVKDAKSILEDSS 120
             PA++D+R L++S  G+AVARMLRS  EFGAFRIVNHGISGEE+LSVV +AKS+LEDS+
Sbjct: 72  TTPAVVDFRSLVSSGCGEAVARMLRSVNEFGAFRIVNHGISGEEVLSVVNEAKSVLEDSN 131

Query: 121 S-------ERNDGARAAIVQVRRRRHGGASEHSVARDEAYRHFSGEMEKVARKVEGIGEK 180
                   + +DG R AI+QVRR      S ++V   E  R  S +MEK+ RK+EGIGEK
Sbjct: 132 KGVDDRRWDGDDGNREAILQVRRPNDSEVSGNTVVEAETNREISEKMEKIRRKLEGIGEK 191

Query: 181 LSEILSESMGEEWGEDQKVKKKRGEKEAILSIFRYNNNQQNQF----DDGGERENEERES 240
           LSEIL   +GE       V+K   +KE I SI+RY+++  + F    D   +    ERES
Sbjct: 192 LSEILCGFVGEN------VEKLGEKKETIFSIYRYHHHPNDLFERKKDHNTKFSKNERES 251

Query: 241 DESVMMSLHIPAEHCQFSVN----PHTQGSFCFDSAADTIVVTIGKQLQEWSMGKLKSAR 300
           DE VMM L IP EHCQF VN       Q S CFD+AADTIVVTIGKQ QE S+GKLKSAR
Sbjct: 252 DEKVMMKLEIPGEHCQFYVNYSCQQQKQYSLCFDAAADTIVVTIGKQFQEMSIGKLKSAR 311

Query: 301 SKMIFVPN--GSQSPFSIELKISHPKLL-----HNPHSNIISISDQIFIALLLFSLYLLY 345
           S+MIFVP+  G+Q+ FSI+LK S+P LL     +N HS IISISDQIF+A LL SLY LY
Sbjct: 312 SEMIFVPDLLGTQTSFSIDLKFSNPNLLLSNNNNNSHSKIISISDQIFVAFLLLSLYFLY 371

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BLAST of MC00g0473 vs. NCBI nr
Match: XP_011653719.1 (uncharacterized protein LOC101207912 [Cucumis sativus] >KAE8649590.1 hypothetical protein Csa_012180 [Cucumis sativus])

HSP 1 Score: 356 bits (913), Expect = 5.80e-118
Identity = 226/374 (60.43%), Postives = 264/374 (70.59%), Query Frame = 0


Query: 1   MALLRTKSRLTIPAPPPSPIPTGTGSRSAANETFKEFLETKSIQLPQLSLPESRFVSGAN 60
           M+LLRT+S LTIPAPPPSPIPTGTGSRSAANETFK FL+  S  LPQLSLPESRF S  N
Sbjct: 12  MSLLRTQSHLTIPAPPPSPIPTGTGSRSAANETFKTFLDN-STHLPQLSLPESRFFSAHN 71

Query: 61  PLPALLDYR-LLASPDGDAVARMLRSAGEFGAFRIVNHGISGEEILSVVKDAKSI--LED 120
           P PA+LD++ L++S   D VARMLRS  EFGAFRIVNHGISGEE+LSVV  AKS+  LED
Sbjct: 72  PAPAVLDFQSLVSSGCADVVARMLRSVHEFGAFRIVNHGISGEEVLSVVNQAKSVSVLED 131

Query: 121 SSS-------ERNDGARAAIVQVRRRRHGGASEHSVARDEAYRHFSGEMEKVARKVEGIG 180
           S+        + +DG R AI+QVRR      S ++V   E  R  S +MEK+ RK+EGIG
Sbjct: 132 SNKGVDDRSWDGDDGNREAILQVRRLNDSEVSGNTVVEAETNREISQKMEKIRRKLEGIG 191

Query: 181 EKLSEILSESMGEEWGEDQKVKKKRGEKEAILSIFRYNNNQQNQFDDGGERENE------ 240
           EKLSEIL   MGE       V+K   +KE + SI+RYNNN  N+ +D  EREN+      
Sbjct: 192 EKLSEILCGFMGEN------VEKLGDKKETMFSIYRYNNNN-NRPNDIFERENDHNTKIS 251

Query: 241 --ERESDESVMMSLHIPAEHCQF--SVNPHTQGSF--CFDSAADTIVVTIGKQLQEWSMG 300
             ERE DESVMM L IP EHCQF  S + H Q  +  CFD+AADTIVVTIGKQ QE SMG
Sbjct: 252 KSEREGDESVMMKLEIPGEHCQFYVSYSCHQQKQYTRCFDAAADTIVVTIGKQFQEMSMG 311

Query: 301 KLKSARSKMIFVPN--GSQSPFSIELKISHPKLL-----HNPHSNIISISDQIFIALLLF 345
           KLKSARS+MIFVP+  G+Q+ FSI+LK S+P LL     +N HS +ISISDQIF A LL 
Sbjct: 312 KLKSARSEMIFVPDLLGTQTSFSIDLKFSNPNLLLNNNNNNSHSKVISISDQIFFAFLLL 371

[Close]

BLAST of MC00g0473 vs. NCBI nr
Match: XP_022950423.1 (uncharacterized protein LOC111453527 [Cucurbita moschata])

HSP 1 Score: 351 bits (900), Expect = 1.47e-116
Identity = 215/354 (60.73%), Postives = 256/354 (72.32%), Query Frame = 0


Query: 1   MALLRTKSRLTIPAPPPSPIPTGTGSRSAANETFKEFLETKSIQLPQLSLPESRFVSGAN 60
           MAL RTKSRLTIPAPPPSPIPTGTGSRSAANETFK+FLE KSI LPQLSLPESRF+S  N
Sbjct: 1   MALSRTKSRLTIPAPPPSPIPTGTGSRSAANETFKQFLE-KSIHLPQLSLPESRFISTTN 60

Query: 61  PLPALLDYRLLASPDG-DAVARMLRSAGEFGAFRIVNHGISGEEILSVVKDAKSILEDS- 120
           P  A++D+R LASP G DA ARMLRS  EFGAFRIVNHGISGEEILSVV +AKS+ ED  
Sbjct: 61  PSLAVIDFRSLASPSGGDATARMLRSVNEFGAFRIVNHGISGEEILSVVNEAKSVWEDDD 120

Query: 121 -SSERNDGARAAIVQVRRRRHGGASEHSVARDEAYRHFSGEMEKVARKVEGIGEKLSEIL 180
            S   + G R  + QVRRR    ASE++V +    R  S +MEK+  K+EGI EK+SE L
Sbjct: 121 RSWAGDVGNREVVFQVRRRNDSEASENTVVQAATNRQISEKMEKIRSKLEGIEEKISERL 180

Query: 181 SESMGEEWGEDQKVKKKRGEKEAILSIFRYNNNQQNQFDDGGERENEERESDESVMMSLH 240
            + MGE         KK  +KE I SI+RYNNN QN   +  EREN+ +      MMSLH
Sbjct: 181 WKCMGENM-------KKGDKKETIFSIYRYNNNHQNP--NPCERENKNKN-----MMSLH 240

Query: 241 IPAEHCQFSVNPHTQ-GSFCFDSAADTIVVTIGKQLQEWSMGKLKSARSKMIFVPN--GS 300
           IP EHCQFS+N H Q  SF FD+AADTIVVT+G+QL E S+GKLKSARS+M FVP+  GS
Sbjct: 241 IPVEHCQFSINSHQQPSSFSFDAAADTIVVTLGEQLVEQSLGKLKSARSEMTFVPDLLGS 300

Query: 301 QSPFSIELKISHPKLLH-NPHSNIISISDQIFIALLLFSLYLLYTYTSSLFKAK 347
            + FSIEL++S+  L+  + HS II+I DQI +A  + S+Y+LY +  SLFK K
Sbjct: 301 GTSFSIELEVSNTNLVKKHSHSKIITIYDQILVAFYVISVYVLYNFMCSLFKGK 339

[Close]

BLAST of MC00g0473 vs. ExPASy TrEMBL
Match: A0A6J1CSG7 (uncharacterized protein LOC111013795 OS=Momordica charantia OX=3673 GN=LOC111013795 PE=4 SV=1)

HSP 1 Score: 662 bits (1709), Expect = 8.19e-240
Identity = 347/347 (100.00%), Postives = 347/347 (100.00%), Query Frame = 0


Query: 1   MALLRTKSRLTIPAPPPSPIPTGTGSRSAANETFKEFLETKSIQLPQLSLPESRFVSGAN 60
           MALLRTKSRLTIPAPPPSPIPTGTGSRSAANETFKEFLETKSIQLPQLSLPESRFVSGAN
Sbjct: 1   MALLRTKSRLTIPAPPPSPIPTGTGSRSAANETFKEFLETKSIQLPQLSLPESRFVSGAN 60

Query: 61  PLPALLDYRLLASPDGDAVARMLRSAGEFGAFRIVNHGISGEEILSVVKDAKSILEDSSS 120
           PLPALLDYRLLASPDGDAVARMLRSAGEFGAFRIVNHGISGEEILSVVKDAKSILEDSSS
Sbjct: 61  PLPALLDYRLLASPDGDAVARMLRSAGEFGAFRIVNHGISGEEILSVVKDAKSILEDSSS 120

Query: 121 ERNDGARAAIVQVRRRRHGGASEHSVARDEAYRHFSGEMEKVARKVEGIGEKLSEILSES 180
           ERNDGARAAIVQVRRRRHGGASEHSVARDEAYRHFSGEMEKVARKVEGIGEKLSEILSES
Sbjct: 121 ERNDGARAAIVQVRRRRHGGASEHSVARDEAYRHFSGEMEKVARKVEGIGEKLSEILSES 180

Query: 181 MGEEWGEDQKVKKKRGEKEAILSIFRYNNNQQNQFDDGGERENEERESDESVMMSLHIPA 240
           MGEEWGEDQKVKKKRGEKEAILSIFRYNNNQQNQFDDGGERENEERESDESVMMSLHIPA
Sbjct: 181 MGEEWGEDQKVKKKRGEKEAILSIFRYNNNQQNQFDDGGERENEERESDESVMMSLHIPA 240

Query: 241 EHCQFSVNPHTQGSFCFDSAADTIVVTIGKQLQEWSMGKLKSARSKMIFVPNGSQSPFSI 300
           EHCQFSVNPHTQGSFCFDSAADTIVVTIGKQLQEWSMGKLKSARSKMIFVPNGSQSPFSI
Sbjct: 241 EHCQFSVNPHTQGSFCFDSAADTIVVTIGKQLQEWSMGKLKSARSKMIFVPNGSQSPFSI 300

Query: 301 ELKISHPKLLHNPHSNIISISDQIFIALLLFSLYLLYTYTSSLFKAK 347
           ELKISHPKLLHNPHSNIISISDQIFIALLLFSLYLLYTYTSSLFKAK
Sbjct: 301 ELKISHPKLLHNPHSNIISISDQIFIALLLFSLYLLYTYTSSLFKAK 347

[Close]

BLAST of MC00g0473 vs. ExPASy TrEMBL
Match: A0A1S3CIU6 (uncharacterized protein LOC103501456 OS=Cucumis melo OX=3656 GN=LOC103501456 PE=4 SV=1)

HSP 1 Score: 367 bits (941), Expect = 1.36e-122
Identity = 225/368 (61.14%), Postives = 262/368 (71.20%), Query Frame = 0


Query: 1   MALLRTKSRLTIPAPPPSPIPTGTGSRSAANETFKEFLETKSIQLPQLSLPESRFVSGAN 60
           MALLRTKSRLTIPAPPPSPIPT TGSRSA NETFK FLE  S  LPQLSLPESRF SG N
Sbjct: 12  MALLRTKSRLTIPAPPPSPIPTATGSRSAGNETFKTFLEN-STHLPQLSLPESRFASGFN 71

Query: 61  PLPALLDYR-LLASPDGDAVARMLRSAGEFGAFRIVNHGISGEEILSVVKDAKSILEDSS 120
             PA++D+R L++S  G+AVARMLRS  EFGAFRIVNHGISGEE+LSVV +AKS+LEDS+
Sbjct: 72  TTPAVVDFRSLVSSGCGEAVARMLRSVNEFGAFRIVNHGISGEEVLSVVNEAKSVLEDSN 131

Query: 121 S-------ERNDGARAAIVQVRRRRHGGASEHSVARDEAYRHFSGEMEKVARKVEGIGEK 180
                   + +DG R AI+QVRR      S ++V   E  R  S +MEK+ RK+EGIGEK
Sbjct: 132 KGVDDRRWDGDDGNREAILQVRRPNDSEVSGNTVVEAETNREISEKMEKIRRKLEGIGEK 191

Query: 181 LSEILSESMGEEWGEDQKVKKKRGEKEAILSIFRYNNNQQNQF----DDGGERENEERES 240
           LSEIL   +GE       V+K   +KE I SI+RY+++  + F    D   +    ERES
Sbjct: 192 LSEILCGFVGEN------VEKLGEKKETIFSIYRYHHHPNDLFERKKDHNTKFSKNERES 251

Query: 241 DESVMMSLHIPAEHCQFSVN----PHTQGSFCFDSAADTIVVTIGKQLQEWSMGKLKSAR 300
           DE VMM L IP EHCQF VN       Q S CFD+AADTIVVTIGKQ QE S+GKLKSAR
Sbjct: 252 DEKVMMKLEIPGEHCQFYVNYSCQQQKQYSLCFDAAADTIVVTIGKQFQEMSIGKLKSAR 311

Query: 301 SKMIFVPN--GSQSPFSIELKISHPKLL-----HNPHSNIISISDQIFIALLLFSLYLLY 345
           S+MIFVP+  G+Q+ FSI+LK S+P LL     +N HS IISISDQIF+A LL SLY LY
Sbjct: 312 SEMIFVPDLLGTQTSFSIDLKFSNPNLLLSNNNNNSHSKIISISDQIFVAFLLLSLYFLY 371

[Close]

BLAST of MC00g0473 vs. ExPASy TrEMBL
Match: A0A6J1GEV0 (uncharacterized protein LOC111453527 OS=Cucurbita moschata OX=3662 GN=LOC111453527 PE=4 SV=1)

HSP 1 Score: 351 bits (900), Expect = 7.11e-117
Identity = 215/354 (60.73%), Postives = 256/354 (72.32%), Query Frame = 0


Query: 1   MALLRTKSRLTIPAPPPSPIPTGTGSRSAANETFKEFLETKSIQLPQLSLPESRFVSGAN 60
           MAL RTKSRLTIPAPPPSPIPTGTGSRSAANETFK+FLE KSI LPQLSLPESRF+S  N
Sbjct: 1   MALSRTKSRLTIPAPPPSPIPTGTGSRSAANETFKQFLE-KSIHLPQLSLPESRFISTTN 60

Query: 61  PLPALLDYRLLASPDG-DAVARMLRSAGEFGAFRIVNHGISGEEILSVVKDAKSILEDS- 120
           P  A++D+R LASP G DA ARMLRS  EFGAFRIVNHGISGEEILSVV +AKS+ ED  
Sbjct: 61  PSLAVIDFRSLASPSGGDATARMLRSVNEFGAFRIVNHGISGEEILSVVNEAKSVWEDDD 120

Query: 121 -SSERNDGARAAIVQVRRRRHGGASEHSVARDEAYRHFSGEMEKVARKVEGIGEKLSEIL 180
            S   + G R  + QVRRR    ASE++V +    R  S +MEK+  K+EGI EK+SE L
Sbjct: 121 RSWAGDVGNREVVFQVRRRNDSEASENTVVQAATNRQISEKMEKIRSKLEGIEEKISERL 180

Query: 181 SESMGEEWGEDQKVKKKRGEKEAILSIFRYNNNQQNQFDDGGERENEERESDESVMMSLH 240
            + MGE         KK  +KE I SI+RYNNN QN   +  EREN+ +      MMSLH
Sbjct: 181 WKCMGENM-------KKGDKKETIFSIYRYNNNHQNP--NPCERENKNKN-----MMSLH 240

Query: 241 IPAEHCQFSVNPHTQ-GSFCFDSAADTIVVTIGKQLQEWSMGKLKSARSKMIFVPN--GS 300
           IP EHCQFS+N H Q  SF FD+AADTIVVT+G+QL E S+GKLKSARS+M FVP+  GS
Sbjct: 241 IPVEHCQFSINSHQQPSSFSFDAAADTIVVTLGEQLVEQSLGKLKSARSEMTFVPDLLGS 300

Query: 301 QSPFSIELKISHPKLLH-NPHSNIISISDQIFIALLLFSLYLLYTYTSSLFKAK 347
            + FSIEL++S+  L+  + HS II+I DQI +A  + S+Y+LY +  SLFK K
Sbjct: 301 GTSFSIELEVSNTNLVKKHSHSKIITIYDQILVAFYVISVYVLYNFMCSLFKGK 339

[Close]

BLAST of MC00g0473 vs. ExPASy TrEMBL
Match: A0A6J1ITI2 (uncharacterized protein LOC111478267 OS=Cucurbita maxima OX=3661 GN=LOC111478267 PE=4 SV=1)

HSP 1 Score: 340 bits (872), Expect = 1.24e-112
Identity = 213/355 (60.00%), Postives = 252/355 (70.99%), Query Frame = 0


Query: 1   MALLRTKSRLTIPAPPPSPIPTGTGSRSAANETFKEFLETKSIQLPQLSLPESRFVSGAN 60
           MAL RTKSRLTIPAPPPSPIPTGTGSRSAANETFK+FLE KSI LPQLSLPESRF+S  N
Sbjct: 1   MALSRTKSRLTIPAPPPSPIPTGTGSRSAANETFKQFLE-KSIHLPQLSLPESRFISTTN 60

Query: 61  PLPALLDYRLLASPDG-DAVARMLRSAGEFGAFRIVNHGISGEEILSVVKDAKSILEDS- 120
           P  A++D+R LASP G DA ARMLRSA EFGAFRIVNHGISGEEILSVV +AKS+ ED  
Sbjct: 61  PSLAVIDFRSLASPSGGDATARMLRSANEFGAFRIVNHGISGEEILSVVNEAKSVWEDDD 120

Query: 121 -SSERNDGARAAIVQVRRRRHGGASEHSVARDEAYRHFSGEMEKVARKVEGIGEKLSEIL 180
            S   + G R  + QVRRR    AS  +V +    R  S +MEK+  K+EGI EK+SE L
Sbjct: 121 RSWAGDVGNRDVVFQVRRRNDSDASAITVVQAATNRQISEKMEKIRSKLEGIEEKISERL 180

Query: 181 SESMGEEWGEDQKVKKKRGEKEAILSIFRYNNNQQNQFDDGGERENEERESDESVMMSLH 240
            E MGE         KK  +KE I SI+RYNN+Q     +  ER+N+ +      MMSLH
Sbjct: 181 WECMGENM-------KKGDKKETIFSIYRYNNHQN---PNPCERDNKNKN-----MMSLH 240

Query: 241 IPAEHCQFSVNPHTQ--GSFCFDSAADTIVVTIGKQLQEWSMGKLKSARSKMIFVPN--G 300
           IP EHCQFS+N H Q   SF FD+AADTIVVT+G+QL E S  KLKSARS+M FVP+  G
Sbjct: 241 IPGEHCQFSINSHQQPSSSFTFDAAADTIVVTLGEQLVERSSEKLKSARSEMTFVPDLLG 300

Query: 301 SQSPFSIELKISHPKLLH-NPHSNIISISDQIFIALLLFSLYLLYTYTSSLFKAK 347
           S + FSIEL++S+  L+  + HSNII+I DQI +A  + S+Y+LY    SLFK K
Sbjct: 301 SGTSFSIELQVSNTNLVKKHSHSNIITIYDQIMVAFYVISVYVLYNCMCSLFKGK 339

[Close]

BLAST of MC00g0473 vs. ExPASy TrEMBL
Match: A0A5J4ZG41 (Uncharacterized protein OS=Nyssa sinensis OX=561372 GN=F0562_017845 PE=4 SV=1)

HSP 1 Score: 219 bits (558), Expect = 2.61e-65
Identity = 150/351 (42.74%), Postives = 211/351 (60.11%), Query Frame = 0


Query: 1   MALLRTKSRLTIPAPPPSPIPTGTGSRSAANETFKEFLETKSIQLPQLSLPESRFVSGAN 60
           MA++RTKS L+ PAPPPSPIPTG G RSAA+ TF E+++ KSIQ+P+L LPE        
Sbjct: 1   MAIMRTKSLLSSPAPPPSPIPTGKGVRSAADSTFSEYID-KSIQIPELILPECLH----R 60

Query: 61  PLPALLDYRLLASPDGDAVARMLRSAGEFGAFRIVNHGISGEEILSVVKDAKSILEDSSS 120
            +PA +DY+ L S DGD+V R+LRSA EFG  RI  HGI  ++I S +   +S+   S  
Sbjct: 61  LVPANIDYQSLVSRDGDSVKRLLRSAAEFGVIRISGHGILADQIRSTLDKTESVFRISDG 120

Query: 121 ERNDGARAAIVQ---VRRRRHGGASE--HSVARDEAYRHFSGEMEKVARKVEGIGEKLSE 180
            + +  R  +     V RR     +E    V   E YR FS EME V  K+E I E+L+ 
Sbjct: 121 RKTEYCRHYVDSEEFVWRRSDIAMAEKCQDVLAAEKYRSFSQEMENVTSKLEAIAEELAL 180

Query: 181 ILSESMGEEWGEDQKVKKKRGEKEAILSIFRYNNNQQNQFDDGGERENEERESDESVMMS 240
           ++SE+  ++       +KK   +E+ILS++RYN +  ++ D      NEE        +S
Sbjct: 181 VISENASKQ------PRKKIQPRESILSLYRYNRH--SRMDQNPSLSNEEIRESCGYALS 240

Query: 241 LHIPAEHCQFSVNPHTQGSFCFDSAADTIVVTIGKQLQEWSMGKLKSARSKMIFVP--NG 300
           LH+  E C+FSV   +  S  F+++ DTIVVTIG+QL+EWS+G  KSA  ++I  P  +G
Sbjct: 241 LHLIVEQCEFSVQSESS-SMSFNTSPDTIVVTIGEQLEEWSLGVFKSASEQVICEPKLHG 300

Query: 301 SQSPFSIELKISHPKLLHNPHSNI---ISISDQIFIALLLFSLYLLYTYTS 341
           S+  FS+ELK   PK  ++ HS I   ISI+DQI I LL+  LY ++ + S
Sbjct: 301 SRPSFSVELKC-WPKNFNHGHSKINKTISIADQILIILLIAFLYKIFVFIS 336

[Close]

BLAST of MC00g0473 vs. TAIR 10
Match: AT2G38500.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 192.2 bits (487), Expect = 7.0e-49
Identity = 143/364 (39.29%), Postives = 208/364 (57.14%), Query Frame = 0


Query: 1   MALLRTKSRLTI-----PAPPPSPIPTGTGSRSAANETFKEFLETKSIQLPQLSLPES-- 60
           MAL+RT+S+L +     P PPPSPIP   GSR AA+E   E +E +SIQ+P+L+LPES  
Sbjct: 1   MALMRTRSQLNVSSLTPPPPPPSPIPRARGSRCAASEILTEIIE-RSIQVPELTLPESHS 60

Query: 61  --RFVSGANPLPALLDYRLLASPDGDAVARMLRSAGEFGAFRIVNHGISGEEILSVVKDA 120
                   + +PA +D+RLLAS    +V R++RSA EFGAFR+  HGISGEE+ S+V+++
Sbjct: 61  GGESCGSRHLIPAEIDFRLLASRREGSVDRLVRSAREFGAFRVSYHGISGEELRSLVRES 120

Query: 121 KSIL-----EDSSSERN-DGARAAIVQVRRRRHGGASEHSVARDEAYRHFSGEMEKVARK 180
             +       D+   R+  G R  IV VR  +            E YR FS EME VA K
Sbjct: 121 GRVFGVLEGRDTGFHRSVVGNRDEIVWVRSWKERMEWAREYIGPERYRCFSQEMENVADK 180

Query: 181 VEGIGEKLSEILSESMGEEWGEDQKVKKKRGEKEAILSIFRYNNNQQNQFDDGGERENEE 240
           +E I  KL +I+ E+       D+K+++     E++LS++RYN+  +N  +       E 
Sbjct: 181 LEDIARKLGQIMVENSRRP--NDKKIQR----GESVLSVYRYNH--ENVTEQSPPLPKER 240

Query: 241 RESDESVMMSLHIPAEHCQFSVNPHTQGSFCFDSAADTIVVTIGKQLQEWSMGKLKSARS 300
            E      +SLH+PA++C+F VN   +G   F +  DTI+VT G+QL+EWS+G+ K  + 
Sbjct: 241 TEEMLHYTLSLHLPAKNCEFRVN-SGKGPLSFHADPDTILVTFGRQLEEWSLGEFKCRQG 300

Query: 301 KMIFVPN--GSQSPFSIELKISHPKLLHN---PHSNIISISDQIFIA-LLLFSLYLLYTY 344
           ++I+ P+  GS + FS+ELK     L H      S   S++ QIF A LLLF     + Y
Sbjct: 301 EIIYHPDAYGSPTSFSVELKCMSLFLSHTSIATTSKTFSLTHQIFTAFLLLFFFQCFWIY 354

The following BLAST results are available for this feature:

BLAST of MC00g0473 vs.


Match Name	E-value	Identity	Description

BLAST of MC00g0473 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022144007.1	1.69e-239	100.00	uncharacterized protein LOC111013795 [Momordica charantia]	[more]
XP_038881407.1	3.91e-129	63.69	uncharacterized protein LOC120072944, partial [Benincasa hispida]	[more]
XP_008463252.1	2.82e-122	61.14	PREDICTED: uncharacterized protein LOC103501456 [Cucumis melo]	[more]
XP_011653719.1	5.80e-118	60.43	uncharacterized protein LOC101207912 [Cucumis sativus] >KAE8649590.1 hypothetica...	[more]
XP_022950423.1	1.47e-116	60.73	uncharacterized protein LOC111453527 [Cucurbita moschata]	[more]

BLAST of MC00g0473 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CSG7	8.19e-240	100.00	uncharacterized protein LOC111013795 OS=Momordica charantia OX=3673 GN=LOC111013...	[more]
A0A1S3CIU6	1.36e-122	61.14	uncharacterized protein LOC103501456 OS=Cucumis melo OX=3656 GN=LOC103501456 PE=...	[more]
A0A6J1GEV0	7.11e-117	60.73	uncharacterized protein LOC111453527 OS=Cucurbita moschata OX=3662 GN=LOC1114535...	[more]
A0A6J1ITI2	1.24e-112	60.00	uncharacterized protein LOC111478267 OS=Cucurbita maxima OX=3661 GN=LOC111478267...	[more]
A0A5J4ZG41	2.61e-65	42.74	Uncharacterized protein OS=Nyssa sinensis OX=561372 GN=F0562_017845 PE=4 SV=1	[more]

BLAST of MC00g0473 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G38500.1	7.0e-49	39.29	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 63..319 e-value: 1.0E-12 score: 50.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..30
None	No IPR available	PANTHER	PTHR34945:SF4	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN	coord: 1..339
None	No IPR available	PANTHER	PTHR34945	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN	coord: 1..339
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 65..284

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC00g0473.1	MC00g0473.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane