MC00g0262 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC00g0262
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Calcium-binding EF-hand family protein
Location	scaffold25: 851049 .. 851603 (+)
RNA-Seq Expression	MC00g0262
Synteny	MC00g0262

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGCTCATCAAAAACTTCAGCCCCAAACGGCTCTTCGGCTCCAAGAAGGAGCGGTCCAAAGTGACCCGGTCCGACCCGCCGTCCTTCGCCTCGTCGTCCTCCTCCTCCGCCGCCACCCCCACCAGCGTCCTCCCTCGCGCCCTCTCCGCCGACGACTGGCCCTCCGACTTCTCCTCCGACATGGCCCGCCACAGCCGAGTCTCCAGGAAACAGCTCGAGGCTCTCCTCGGCCTGTCCGGGGCTGACCCGCCGAGCCACGAGGAGGTGGCGGCGATGCTCAGCGAACTCGACCGCCACGGGGACGGCGTCGTCAGCGTCGAGGCGCTGATGAGCCACGTAGGAGGCGCCTGCGACGAGGATCTCCGAGAGACGTTCGAGATCTTCGACGCGGACGGCGACGGGAGGATTACGGCGGAGGAGCTGTTCAGCGTGTTCGTGGCCATTGGGGAGGAGGAGCGGTGCACGTTAGAGGACTGCCGACGCATGATAGCCGGAGTCGACAAGAACGGGGATGGGTTCGTCTGCTTCGAGGACTTTGCGCGTATGATGGAC

mRNA sequence

ATGAAGCTCATCAAAAACTTCAGCCCCAAACGGCTCTTCGGCTCCAAGAAGGAGCGGTCCAAAGTGACCCGGTCCGACCCGCCGTCCTTCGCCTCGTCGTCCTCCTCCTCCGCCGCCACCCCCACCAGCGTCCTCCCTCGCGCCCTCTCCGCCGACGACTGGCCCTCCGACTTCTCCTCCGACATGGCCCGCCACAGCCGAGTCTCCAGGAAACAGCTCGAGGCTCTCCTCGGCCTGTCCGGGGCTGACCCGCCGAGCCACGAGGAGGTGGCGGCGATGCTCAGCGAACTCGACCGCCACGGGGACGGCGTCGTCAGCGTCGAGGCGCTGATGAGCCACGTAGGAGGCGCCTGCGACGAGGATCTCCGAGAGACGTTCGAGATCTTCGACGCGGACGGCGACGGGAGGATTACGGCGGAGGAGCTGTTCAGCGTGTTCGTGGCCATTGGGGAGGAGGAGCGGTGCACGTTAGAGGACTGCCGACGCATGATAGCCGGAGTCGACAAGAACGGGGATGGGTTCGTCTGCTTCGAGGACTTTGCGCGTATGATGGAC

Coding sequence (CDS)

ATGAAGCTCATCAAAAACTTCAGCCCCAAACGGCTCTTCGGCTCCAAGAAGGAGCGGTCCAAAGTGACCCGGTCCGACCCGCCGTCCTTCGCCTCGTCGTCCTCCTCCTCCGCCGCCACCCCCACCAGCGTCCTCCCTCGCGCCCTCTCCGCCGACGACTGGCCCTCCGACTTCTCCTCCGACATGGCCCGCCACAGCCGAGTCTCCAGGAAACAGCTCGAGGCTCTCCTCGGCCTGTCCGGGGCTGACCCGCCGAGCCACGAGGAGGTGGCGGCGATGCTCAGCGAACTCGACCGCCACGGGGACGGCGTCGTCAGCGTCGAGGCGCTGATGAGCCACGTAGGAGGCGCCTGCGACGAGGATCTCCGAGAGACGTTCGAGATCTTCGACGCGGACGGCGACGGGAGGATTACGGCGGAGGAGCTGTTCAGCGTGTTCGTGGCCATTGGGGAGGAGGAGCGGTGCACGTTAGAGGACTGCCGACGCATGATAGCCGGAGTCGACAAGAACGGGGATGGGTTCGTCTGCTTCGAGGACTTTGCGCGTATGATGGAC

Protein sequence

MKLIKNFSPKRLFGSKKERSKVTRSDPPSFASSSSSSAATPTSVLPRALSADDWPSDFSSDMARHSRVSRKQLEALLGLSGADPPSHEEVAAMLSELDRHGDGVVSVEALMSHVGGACDEDLRETFEIFDADGDGRITAEELFSVFVAIGEEERCTLEDCRRMIAGVDKNGDGFVCFEDFARMMD

Homology

[Close]

BLAST of MC00g0262 vs. ExPASy Swiss-Prot
Match: P30188 (Probable calcium-binding protein CML35 OS=Arabidopsis thaliana OX=3702 GN=CML35 PE=2 SV=2)

HSP 1 Score: 142.9 bits (359), Expect = 3.6e-33
Identity = 96/205 (46.83%), Postives = 124/205 (60.49%), Query Frame = 0


Query: 4   IKNFSPKRLFGSKKERSKVTRSDPPSFASSSSSSA-------------ATPTSVLPRALS 63
           +   SPKRLF +K + S V+RS+P SF+S++SSS+             ATP SVLP+  +
Sbjct: 7   LNRLSPKRLFRTKSKAS-VSRSEPSSFSSNASSSSSDGSYGNLKQGPTATPISVLPQ--N 66

Query: 64  ADDWPSDFSS-----DMARHSRVSRKQLEALLGLSGADPPSHEEVAAMLSELDRHGDGVV 123
           + D+ ++        D      VSR  L AL+     +PPS EEV+ ML E+D    G +
Sbjct: 67  SGDFYTELVQAFKLIDRDDDGVVSRGDLAALISRLSHEPPSQEEVSLMLREVDGGDGGCI 126

Query: 124 SVEALMSHVGGAC------DEDLRETFEIFDADGDGRITAEELFSVFVAIGEEERCTLED 183
           S+E L S V G         E+LRE FEIFD D +G+I+AEEL  VF  IG +ERCTLE+
Sbjct: 127 SLEDLASRVAGTSGEGSVETEELREVFEIFDVDRNGKISAEELHRVFGVIG-DERCTLEE 186

Query: 184 CRRMIAGVDKNGDGFVCFEDFARMM 185
           C RMIA VD NGDGFVCF+DF RMM
Sbjct: 187 CMRMIATVDGNGDGFVCFDDFCRMM 207

[Close]

BLAST of MC00g0262 vs. ExPASy Swiss-Prot
Match: Q9SS31 (Probable calcium-binding protein CML36 OS=Arabidopsis thaliana OX=3702 GN=CML36 PE=2 SV=1)

HSP 1 Score: 139.8 bits (351), Expect = 3.1e-32
Identity = 95/208 (45.67%), Postives = 120/208 (57.69%), Query Frame = 0


Query: 1   MKLIKNFSPKRLFGSKKERSKVTRSDPP-SFASSSSSS--------------AATPTSVL 60
           MKL K   PKR F   K+RS V++S    SF S+SSSS              + TPTS+L
Sbjct: 1   MKLAK-LIPKRFFIRSKDRSTVSKSPTAFSFGSASSSSGQDCKNSGGDGGGGSVTPTSIL 60

Query: 61  PRALSADDWPSDFSS----DMARHSRVSRKQLEALLGLSGADPPSHEEVAAMLSELDRHG 120
           P   S   +     +    D      VSR  LE+LL   G DP + EE+  ML E+D  G
Sbjct: 61  PEVPSPYSYVEILQAFKLIDRDNDGAVSRHDLESLLSRLGPDPLTEEEINVMLKEVDCDG 120

Query: 121 DGVVSVEALMSHV----GGACDEDLRETFEIFDADGDGRITAEELFSVFVAIGEEERCTL 180
           DG + +E L S V          +L+ETFE FDAD DG I+A+EL  VF  IG +ERCTL
Sbjct: 121 DGTIRLEELASRVVSLDPARDSTELKETFEFFDADRDGLISADELLRVFSTIG-DERCTL 180

Query: 181 EDCRRMIAGVDKNGDGFVCFEDFARMMD 186
           +DC+RMIA VD++GDGFVCF +F+RMMD
Sbjct: 181 DDCKRMIADVDEDGDGFVCFTEFSRMMD 206

[Close]

BLAST of MC00g0262 vs. ExPASy Swiss-Prot
Match: Q8RZB5 (Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica OX=39947 GN=CML10 PE=2 SV=1)

HSP 1 Score: 95.9 bits (237), Expect = 5.1e-19
Identity = 70/181 (38.67%), Postives = 96/181 (53.04%), Query Frame = 0


Query: 10  KRLFGSKKERSKVTRSDPPSFASSSSSSAATPTSVLPRALSADDWPSDFSSDMARHSRVS 69
           +R  GSK     + ++DP   AS   S A TP   + R     D   D         R+S
Sbjct: 11  RRRSGSKS--PPLPQADP---ASGGGSPAPTPEEEMERVFRKFDANGD--------GRIS 70

Query: 70  RKQLEALLGLSGADPPSHEEVAAMLSELDRHGDGVVSVE------ALMSHVGGACDEDLR 129
           R +L AL    G    + +E+A M++E D  GDG +S++      A  S    A +EDLR
Sbjct: 71  RSELGALFESLG-HAATDDELARMMAEADADGDGFISLDEFAALNATASGDAAAVEEDLR 130

Query: 130 ETFEIFDADGDGRITAEELFSVFVAIGEEERCTLEDCRRMIAGVDKNGDGFVCFEDFARM 185
             F +FDADG+G I+A EL  V   +G  E+ T++ CRRMI GVD+NGDG + FE+F  M
Sbjct: 131 HAFRVFDADGNGTISAAELARVLHGLG--EKATVQQCRRMIEGVDQNGDGLISFEEFKVM 175

[Close]

BLAST of MC00g0262 vs. ExPASy Swiss-Prot
Match: Q0JC44 (Probable calcium-binding protein CML22 OS=Oryza sativa subsp. japonica OX=39947 GN=CML22 PE=3 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 4.3e-18
Identity = 56/136 (41.18%), Postives = 85/136 (62.50%), Query Frame = 0


Query: 67  RVSRKQLEALLGLSGADPPSHEEVAAMLSELDRHGDGVVSVE-------ALM-----SHV 126
           R++R++LE  LG  G   P+ +E+AA+++ +D +GDG V VE       ++M     S  
Sbjct: 107 RITREELEDSLGKLGIPVPA-DELAAVIARIDANGDGCVDVEEFGELYRSIMAGGDDSKD 166

Query: 127 GGACDE------DLRETFEIFDADGDGRITAEELFSVFVAIGEEERCTLEDCRRMIAGVD 185
           G A +E      D+RE F +FDA+GDG IT +EL +V  ++G ++  T E+CRRMI  VD
Sbjct: 167 GRAKEEEEEEDGDMREAFRVFDANGDGYITVDELGAVLASLGLKQGRTAEECRRMIGQVD 226

[Close]

BLAST of MC00g0262 vs. ExPASy Swiss-Prot
Match: Q9FYK2 (Probable calcium-binding protein CML25 OS=Arabidopsis thaliana OX=3702 GN=CML25 PE=2 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 5.6e-18
Identity = 49/129 (37.98%), Postives = 80/129 (62.02%), Query Frame = 0


Query: 61  DMARHSRVSRKQLEALLGLSGADPPSHEEVAAMLSELDRHGDGVVSVEALMSHVGGACD- 120
           D+    ++S K+L A++   G + P  EE+   ++E+DR GDG ++ E  +       D 
Sbjct: 46  DVNGDGKISSKELGAIMTSLGHEVP-EEELEKAITEIDRKGDGYINFEEFVELNTKGMDQ 105

Query: 121 ----EDLRETFEIFDADGDGRITAEELFSVFVAIGEEERCTLEDCRRMIAGVDKNGDGFV 180
               E+L++ F ++D DG+G I+AEEL  V  ++G+E  C++ +CR+MI GVDK+GDG +
Sbjct: 106 NDVLENLKDAFSVYDIDGNGSISAEELHEVLRSLGDE--CSIAECRKMIGGVDKDGDGTI 165

Query: 181 CFEDFARMM 185
            FE+F  MM
Sbjct: 166 DFEEFKIMM 171

[Close]

BLAST of MC00g0262 vs. NCBI nr
Match: XP_022155928.1 (probable calcium-binding protein CML35 [Momordica charantia])

HSP 1 Score: 359 bits (922), Expect = 7.15e-125
Identity = 185/185 (100.00%), Postives = 185/185 (100.00%), Query Frame = 0


Query: 1   MKLIKNFSPKRLFGSKKERSKVTRSDPPSFASSSSSSAATPTSVLPRALSADDWPSDFSS 60
           MKLIKNFSPKRLFGSKKERSKVTRSDPPSFASSSSSSAATPTSVLPRALSADDWPSDFSS
Sbjct: 1   MKLIKNFSPKRLFGSKKERSKVTRSDPPSFASSSSSSAATPTSVLPRALSADDWPSDFSS 60

Query: 61  DMARHSRVSRKQLEALLGLSGADPPSHEEVAAMLSELDRHGDGVVSVEALMSHVGGACDE 120
           DMARHSRVSRKQLEALLGLSGADPPSHEEVAAMLSELDRHGDGVVSVEALMSHVGGACDE
Sbjct: 61  DMARHSRVSRKQLEALLGLSGADPPSHEEVAAMLSELDRHGDGVVSVEALMSHVGGACDE 120

Query: 121 DLRETFEIFDADGDGRITAEELFSVFVAIGEEERCTLEDCRRMIAGVDKNGDGFVCFEDF 180
           DLRETFEIFDADGDGRITAEELFSVFVAIGEEERCTLEDCRRMIAGVDKNGDGFVCFEDF
Sbjct: 121 DLRETFEIFDADGDGRITAEELFSVFVAIGEEERCTLEDCRRMIAGVDKNGDGFVCFEDF 180

Query: 181 ARMMD 185
           ARMMD
Sbjct: 181 ARMMD 185

[Close]

BLAST of MC00g0262 vs. NCBI nr
Match: XP_038903370.1 (probable calcium-binding protein CML36 [Benincasa hispida])

HSP 1 Score: 209 bits (531), Expect = 1.05e-65
Identity = 122/186 (65.59%), Postives = 139/186 (74.73%), Query Frame = 0


Query: 1   MKLIKNFSPKRLFGSKKERSKVTRSDPPSFAS-SSSSSAATPTSVLPRALSADDWPSDFS 60
           MKL+K+F+P RLF SK  RSK++RS PPSF S SSSSSA TPTSVLP A+    W SDFS
Sbjct: 1   MKLLKSFNPNRLFSSKSHRSKLSRSQPPSFGSASSSSSAGTPTSVLPSAV----W-SDFS 60

Query: 61  SDMARHSRVSRKQLEALLGLSGADPPSHEEVAAMLSELDRHGDGVVSVEALMSHVGGACD 120
                 S +SRK+LEALLGL GA+PPS EE+  M+  +DR              VG AC 
Sbjct: 61  D-----STLSRKELEALLGLIGAEPPSEEELKTMMGAMDR--------------VGPACH 120

Query: 121 EDLRETFEIFDADGDGRITAEELFSVFVAIGEEERCTLEDCRRMIAGVDKNGDGFVCFED 180
            +LR+TFEIFDAD DGRITAEELFSVF A+G+E+ CTLEDCRRMIAGVDKNGDGFVCFED
Sbjct: 121 SELRDTFEIFDADHDGRITAEELFSVFTAMGDEQ-CTLEDCRRMIAGVDKNGDGFVCFED 161

Query: 181 FARMMD 185
           FARMMD
Sbjct: 181 FARMMD 161

[Close]

BLAST of MC00g0262 vs. NCBI nr
Match: XP_024181636.1 (probable calcium-binding protein CML36 [Rosa chinensis] >PRQ49697.1 putative EF-hand domain pair protein [Rosa chinensis])

HSP 1 Score: 205 bits (522), Expect = 1.45e-63
Identity = 128/220 (58.18%), Postives = 153/220 (69.55%), Query Frame = 0


Query: 1   MKLIK--NFSPKRLFGSKKERSKVTRSDPPSFASSSSSSAA----------------TPT 60
           MKL+K  + SPKRLF S K+RS V+RSDPPSF S ++SS++                TPT
Sbjct: 1   MKLMKINSLSPKRLFRSMKDRSAVSRSDPPSFGSGTASSSSSDPLPSDHKPGPGGMVTPT 60

Query: 61  SVLPRALSADDWPSDFSSD----MARHSR---------VSRKQLEALLGLSGADPPSHEE 120
           SVLP    + DW SDFS+D    MA+  +         VSRK+LEALL   GA+PPSHEE
Sbjct: 61  SVLPEI--SGDW-SDFSADTHLDMAQAFKLIDRDNDGVVSRKELEALLKRLGAEPPSHEE 120

Query: 121 VAAMLSELDRHGDGVVSVEALMSHVGGAC----DEDLRETFEIFDADGDGRITAEELFSV 180
           VA MLSE+DR GDG + +EAL+   G AC    D +LRE FE+FDAD DG+I+AEELFSV
Sbjct: 121 VAMMLSEVDRDGDGCIPLEALLHRFGPACEPAADSELREAFEVFDADHDGKISAEELFSV 180

Query: 181 FVAIGEEERCTLEDCRRMIAGVDKNGDGFVCFEDFARMMD 185
           F AIG++ RCTLEDCRRMIA VDKNGDGFVCFEDFA MM+
Sbjct: 181 FTAIGDD-RCTLEDCRRMIAEVDKNGDGFVCFEDFALMME 216

[Close]

BLAST of MC00g0262 vs. NCBI nr
Match: XP_008443339.2 (PREDICTED: probable calcium-binding protein CML35 [Cucumis melo] >KAA0053814.1 putative calcium-binding protein CML35 [Cucumis melo var. makuwa] >TYK25587.1 putative calcium-binding protein CML35 [Cucumis melo var. makuwa])

HSP 1 Score: 201 bits (512), Expect = 9.28e-63
Identity = 118/190 (62.11%), Postives = 138/190 (72.63%), Query Frame = 0


Query: 1   MKLIKNFSPKRLFGSKKERSKVTRSDPPSF-----ASSSSSSAATPTSVLPRALSADDWP 60
           MKL+K+F+P RLF SK  RSK++RS PPSF     +SSS+SSA TPTSVLP      DW 
Sbjct: 1   MKLLKSFNPNRLFSSKSHRSKLSRSQPPSFGSASSSSSSTSSAGTPTSVLPSL----DW- 60

Query: 61  SDFSSDMARHSRVSRKQLEALLGLSGADPPSHEEVAAMLSELDRHGDGVVSVEALMSHVG 120
           +DFS        +SRK+L+ALLGL GA+PPS EE+  M+ E+DR              VG
Sbjct: 61  ADFSDPT-----LSRKELKALLGLIGAEPPSEEEIKIMMGEMDR--------------VG 120

Query: 121 GACDEDLRETFEIFDADGDGRITAEELFSVFVAIGEEERCTLEDCRRMIAGVDKNGDGFV 180
            AC  +LR+TFEIFDAD DGRITAEELFSVF A+G+E  CTLEDCRRMIAGVDKNGDGFV
Sbjct: 121 PACHSELRDTFEIFDADHDGRITAEELFSVFTAMGDEG-CTLEDCRRMIAGVDKNGDGFV 165

Query: 181 CFEDFARMMD 185
           CF+DFARMMD
Sbjct: 181 CFDDFARMMD 165

[Close]

BLAST of MC00g0262 vs. NCBI nr
Match: XP_012085969.1 (probable calcium-binding protein CML35 [Jatropha curcas] >KDP26218.1 hypothetical protein JCGZ_22464 [Jatropha curcas])

HSP 1 Score: 200 bits (508), Expect = 1.93e-61
Identity = 124/214 (57.94%), Postives = 146/214 (68.22%), Query Frame = 0


Query: 4   IKNFSPKRLFGSKKERSKVTRSDPPSF---ASSSSSS--------------AATPTSVLP 63
           IK  SPKRL GSKK+RS V++SDP S+   ASSSSSS              A TPTSVLP
Sbjct: 5   IKAISPKRLLGSKKDRSIVSKSDPSSYSYGASSSSSSDSSVSNQKRGVTAEAGTPTSVLP 64

Query: 64  RALSAD--DWPSDFSSDMARHSR---------VSRKQLEALLGLSGADPPSHEEVAAMLS 123
             +S D  D  +DF SD+ +  +         VSR++LEALL   GA+PPS EEVA MLS
Sbjct: 65  E-ISGDWSDISTDFYSDLVQAFKLIDRDNDGIVSRRELEALLSRLGAEPPSQEEVATMLS 124

Query: 124 ELDRHGDGVVSVEALMSHVGGAC----DEDLRETFEIFDADGDGRITAEELFSVFVAIGE 183
           E+D  GDG +SVEALM+ +G AC    D++LR  FE FDAD DG+ITAEELF V+ AIG+
Sbjct: 125 EVDHVGDGCISVEALMNRIGSACEPAGDDELRVAFEFFDADQDGKITAEELFGVYKAIGD 184

Query: 184 EERCTLEDCRRMIAGVDKNGDGFVCFEDFARMMD 185
           E RCT EDC RMIA VDKNGDGFVCFEDF RMM+
Sbjct: 185 E-RCTFEDCMRMIASVDKNGDGFVCFEDFCRMME 216

[Close]

BLAST of MC00g0262 vs. ExPASy TrEMBL
Match: A0A6J1DP84 (probable calcium-binding protein CML35 OS=Momordica charantia OX=3673 GN=LOC111022928 PE=4 SV=1)

HSP 1 Score: 359 bits (922), Expect = 3.46e-125
Identity = 185/185 (100.00%), Postives = 185/185 (100.00%), Query Frame = 0


Query: 1   MKLIKNFSPKRLFGSKKERSKVTRSDPPSFASSSSSSAATPTSVLPRALSADDWPSDFSS 60
           MKLIKNFSPKRLFGSKKERSKVTRSDPPSFASSSSSSAATPTSVLPRALSADDWPSDFSS
Sbjct: 1   MKLIKNFSPKRLFGSKKERSKVTRSDPPSFASSSSSSAATPTSVLPRALSADDWPSDFSS 60

Query: 61  DMARHSRVSRKQLEALLGLSGADPPSHEEVAAMLSELDRHGDGVVSVEALMSHVGGACDE 120
           DMARHSRVSRKQLEALLGLSGADPPSHEEVAAMLSELDRHGDGVVSVEALMSHVGGACDE
Sbjct: 61  DMARHSRVSRKQLEALLGLSGADPPSHEEVAAMLSELDRHGDGVVSVEALMSHVGGACDE 120

Query: 121 DLRETFEIFDADGDGRITAEELFSVFVAIGEEERCTLEDCRRMIAGVDKNGDGFVCFEDF 180
           DLRETFEIFDADGDGRITAEELFSVFVAIGEEERCTLEDCRRMIAGVDKNGDGFVCFEDF
Sbjct: 121 DLRETFEIFDADGDGRITAEELFSVFVAIGEEERCTLEDCRRMIAGVDKNGDGFVCFEDF 180

Query: 181 ARMMD 185
           ARMMD
Sbjct: 181 ARMMD 185

[Close]

BLAST of MC00g0262 vs. ExPASy TrEMBL
Match: A0A2P6RTD5 (Putative EF-hand domain pair protein OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr2g0124821 PE=4 SV=1)

HSP 1 Score: 205 bits (522), Expect = 7.02e-64
Identity = 128/220 (58.18%), Postives = 153/220 (69.55%), Query Frame = 0


Query: 1   MKLIK--NFSPKRLFGSKKERSKVTRSDPPSFASSSSSSAA----------------TPT 60
           MKL+K  + SPKRLF S K+RS V+RSDPPSF S ++SS++                TPT
Sbjct: 1   MKLMKINSLSPKRLFRSMKDRSAVSRSDPPSFGSGTASSSSSDPLPSDHKPGPGGMVTPT 60

Query: 61  SVLPRALSADDWPSDFSSD----MARHSR---------VSRKQLEALLGLSGADPPSHEE 120
           SVLP    + DW SDFS+D    MA+  +         VSRK+LEALL   GA+PPSHEE
Sbjct: 61  SVLPEI--SGDW-SDFSADTHLDMAQAFKLIDRDNDGVVSRKELEALLKRLGAEPPSHEE 120

Query: 121 VAAMLSELDRHGDGVVSVEALMSHVGGAC----DEDLRETFEIFDADGDGRITAEELFSV 180
           VA MLSE+DR GDG + +EAL+   G AC    D +LRE FE+FDAD DG+I+AEELFSV
Sbjct: 121 VAMMLSEVDRDGDGCIPLEALLHRFGPACEPAADSELREAFEVFDADHDGKISAEELFSV 180

Query: 181 FVAIGEEERCTLEDCRRMIAGVDKNGDGFVCFEDFARMMD 185
           F AIG++ RCTLEDCRRMIA VDKNGDGFVCFEDFA MM+
Sbjct: 181 FTAIGDD-RCTLEDCRRMIAEVDKNGDGFVCFEDFALMME 216

[Close]

BLAST of MC00g0262 vs. ExPASy TrEMBL
Match: A0A5A7UEZ0 (Putative calcium-binding protein CML35 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G007460 PE=4 SV=1)

HSP 1 Score: 201 bits (512), Expect = 4.49e-63
Identity = 118/190 (62.11%), Postives = 138/190 (72.63%), Query Frame = 0


Query: 1   MKLIKNFSPKRLFGSKKERSKVTRSDPPSF-----ASSSSSSAATPTSVLPRALSADDWP 60
           MKL+K+F+P RLF SK  RSK++RS PPSF     +SSS+SSA TPTSVLP      DW 
Sbjct: 1   MKLLKSFNPNRLFSSKSHRSKLSRSQPPSFGSASSSSSSTSSAGTPTSVLPSL----DW- 60

Query: 61  SDFSSDMARHSRVSRKQLEALLGLSGADPPSHEEVAAMLSELDRHGDGVVSVEALMSHVG 120
           +DFS        +SRK+L+ALLGL GA+PPS EE+  M+ E+DR              VG
Sbjct: 61  ADFSDPT-----LSRKELKALLGLIGAEPPSEEEIKIMMGEMDR--------------VG 120

Query: 121 GACDEDLRETFEIFDADGDGRITAEELFSVFVAIGEEERCTLEDCRRMIAGVDKNGDGFV 180
            AC  +LR+TFEIFDAD DGRITAEELFSVF A+G+E  CTLEDCRRMIAGVDKNGDGFV
Sbjct: 121 PACHSELRDTFEIFDADHDGRITAEELFSVFTAMGDEG-CTLEDCRRMIAGVDKNGDGFV 165

Query: 181 CFEDFARMMD 185
           CF+DFARMMD
Sbjct: 181 CFDDFARMMD 165

[Close]

BLAST of MC00g0262 vs. ExPASy TrEMBL
Match: A0A1S3B7R8 (probable calcium-binding protein CML35 OS=Cucumis melo OX=3656 GN=LOC103486950 PE=4 SV=1)

HSP 1 Score: 201 bits (512), Expect = 4.49e-63
Identity = 118/190 (62.11%), Postives = 138/190 (72.63%), Query Frame = 0


Query: 1   MKLIKNFSPKRLFGSKKERSKVTRSDPPSF-----ASSSSSSAATPTSVLPRALSADDWP 60
           MKL+K+F+P RLF SK  RSK++RS PPSF     +SSS+SSA TPTSVLP      DW 
Sbjct: 1   MKLLKSFNPNRLFSSKSHRSKLSRSQPPSFGSASSSSSSTSSAGTPTSVLPSL----DW- 60

Query: 61  SDFSSDMARHSRVSRKQLEALLGLSGADPPSHEEVAAMLSELDRHGDGVVSVEALMSHVG 120
           +DFS        +SRK+L+ALLGL GA+PPS EE+  M+ E+DR              VG
Sbjct: 61  ADFSDPT-----LSRKELKALLGLIGAEPPSEEEIKIMMGEMDR--------------VG 120

Query: 121 GACDEDLRETFEIFDADGDGRITAEELFSVFVAIGEEERCTLEDCRRMIAGVDKNGDGFV 180
            AC  +LR+TFEIFDAD DGRITAEELFSVF A+G+E  CTLEDCRRMIAGVDKNGDGFV
Sbjct: 121 PACHSELRDTFEIFDADHDGRITAEELFSVFTAMGDEG-CTLEDCRRMIAGVDKNGDGFV 165

Query: 181 CFEDFARMMD 185
           CF+DFARMMD
Sbjct: 181 CFDDFARMMD 165

[Close]

BLAST of MC00g0262 vs. ExPASy TrEMBL
Match: A0A067JTX4 (Uncharacterized protein OS=Jatropha curcas OX=180498 GN=JCGZ_22464 PE=4 SV=1)

HSP 1 Score: 200 bits (508), Expect = 9.33e-62
Identity = 124/214 (57.94%), Postives = 146/214 (68.22%), Query Frame = 0


Query: 4   IKNFSPKRLFGSKKERSKVTRSDPPSF---ASSSSSS--------------AATPTSVLP 63
           IK  SPKRL GSKK+RS V++SDP S+   ASSSSSS              A TPTSVLP
Sbjct: 5   IKAISPKRLLGSKKDRSIVSKSDPSSYSYGASSSSSSDSSVSNQKRGVTAEAGTPTSVLP 64

Query: 64  RALSAD--DWPSDFSSDMARHSR---------VSRKQLEALLGLSGADPPSHEEVAAMLS 123
             +S D  D  +DF SD+ +  +         VSR++LEALL   GA+PPS EEVA MLS
Sbjct: 65  E-ISGDWSDISTDFYSDLVQAFKLIDRDNDGIVSRRELEALLSRLGAEPPSQEEVATMLS 124

Query: 124 ELDRHGDGVVSVEALMSHVGGAC----DEDLRETFEIFDADGDGRITAEELFSVFVAIGE 183
           E+D  GDG +SVEALM+ +G AC    D++LR  FE FDAD DG+ITAEELF V+ AIG+
Sbjct: 125 EVDHVGDGCISVEALMNRIGSACEPAGDDELRVAFEFFDADQDGKITAEELFGVYKAIGD 184

Query: 184 EERCTLEDCRRMIAGVDKNGDGFVCFEDFARMMD 185
           E RCT EDC RMIA VDKNGDGFVCFEDF RMM+
Sbjct: 185 E-RCTFEDCMRMIASVDKNGDGFVCFEDFCRMME 216

[Close]

BLAST of MC00g0262 vs. TAIR 10
Match: AT2G41410.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 142.9 bits (359), Expect = 2.6e-34
Identity = 96/205 (46.83%), Postives = 124/205 (60.49%), Query Frame = 0


Query: 4   IKNFSPKRLFGSKKERSKVTRSDPPSFASSSSSSA-------------ATPTSVLPRALS 63
           +   SPKRLF +K + S V+RS+P SF+S++SSS+             ATP SVLP+  +
Sbjct: 7   LNRLSPKRLFRTKSKAS-VSRSEPSSFSSNASSSSSDGSYGNLKQGPTATPISVLPQ--N 66

Query: 64  ADDWPSDFSS-----DMARHSRVSRKQLEALLGLSGADPPSHEEVAAMLSELDRHGDGVV 123
           + D+ ++        D      VSR  L AL+     +PPS EEV+ ML E+D    G +
Sbjct: 67  SGDFYTELVQAFKLIDRDDDGVVSRGDLAALISRLSHEPPSQEEVSLMLREVDGGDGGCI 126

Query: 124 SVEALMSHVGGAC------DEDLRETFEIFDADGDGRITAEELFSVFVAIGEEERCTLED 183
           S+E L S V G         E+LRE FEIFD D +G+I+AEEL  VF  IG +ERCTLE+
Sbjct: 127 SLEDLASRVAGTSGEGSVETEELREVFEIFDVDRNGKISAEELHRVFGVIG-DERCTLEE 186

Query: 184 CRRMIAGVDKNGDGFVCFEDFARMM 185
           C RMIA VD NGDGFVCF+DF RMM
Sbjct: 187 CMRMIATVDGNGDGFVCFDDFCRMM 207

[Close]

BLAST of MC00g0262 vs. TAIR 10
Match: AT3G10190.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 139.8 bits (351), Expect = 2.2e-33
Identity = 95/208 (45.67%), Postives = 120/208 (57.69%), Query Frame = 0


Query: 1   MKLIKNFSPKRLFGSKKERSKVTRSDPP-SFASSSSSS--------------AATPTSVL 60
           MKL K   PKR F   K+RS V++S    SF S+SSSS              + TPTS+L
Sbjct: 1   MKLAK-LIPKRFFIRSKDRSTVSKSPTAFSFGSASSSSGQDCKNSGGDGGGGSVTPTSIL 60

Query: 61  PRALSADDWPSDFSS----DMARHSRVSRKQLEALLGLSGADPPSHEEVAAMLSELDRHG 120
           P   S   +     +    D      VSR  LE+LL   G DP + EE+  ML E+D  G
Sbjct: 61  PEVPSPYSYVEILQAFKLIDRDNDGAVSRHDLESLLSRLGPDPLTEEEINVMLKEVDCDG 120

Query: 121 DGVVSVEALMSHV----GGACDEDLRETFEIFDADGDGRITAEELFSVFVAIGEEERCTL 180
           DG + +E L S V          +L+ETFE FDAD DG I+A+EL  VF  IG +ERCTL
Sbjct: 121 DGTIRLEELASRVVSLDPARDSTELKETFEFFDADRDGLISADELLRVFSTIG-DERCTL 180

Query: 181 EDCRRMIAGVDKNGDGFVCFEDFARMMD 186
           +DC+RMIA VD++GDGFVCF +F+RMMD
Sbjct: 181 DDCKRMIADVDEDGDGFVCFTEFSRMMD 206

[Close]

BLAST of MC00g0262 vs. TAIR 10
Match: AT1G24620.1 (EF hand calcium-binding protein family )

HSP 1 Score: 92.4 bits (228), Expect = 4.0e-19
Identity = 49/129 (37.98%), Postives = 80/129 (62.02%), Query Frame = 0


Query: 61  DMARHSRVSRKQLEALLGLSGADPPSHEEVAAMLSELDRHGDGVVSVEALMSHVGGACD- 120
           D+    ++S K+L A++   G + P  EE+   ++E+DR GDG ++ E  +       D 
Sbjct: 46  DVNGDGKISSKELGAIMTSLGHEVP-EEELEKAITEIDRKGDGYINFEEFVELNTKGMDQ 105

Query: 121 ----EDLRETFEIFDADGDGRITAEELFSVFVAIGEEERCTLEDCRRMIAGVDKNGDGFV 180
               E+L++ F ++D DG+G I+AEEL  V  ++G+E  C++ +CR+MI GVDK+GDG +
Sbjct: 106 NDVLENLKDAFSVYDIDGNGSISAEELHEVLRSLGDE--CSIAECRKMIGGVDKDGDGTI 165

Query: 181 CFEDFARMM 185
            FE+F  MM
Sbjct: 166 DFEEFKIMM 171

[Close]

BLAST of MC00g0262 vs. TAIR 10
Match: AT3G59440.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 84.3 bits (207), Expect = 1.1e-16
Identity = 46/122 (37.70%), Postives = 74/122 (60.66%), Query Frame = 0


Query: 67  RVSRKQLEALLGLSGADPPSHEEVAAMLSELDRHGDGVVSVEALMSHVGGACDE----DL 126
           R+++++L   L   G   P  +++  M+ ++D +GDG V +    S  G   +E    D+
Sbjct: 66  RITKEELNDSLENLGIFMPD-KDLIQMIQKMDANGDGCVDINEFESLYGSIVEEKEEGDM 125

Query: 127 RETFEIFDADGDGRITAEELFSVFVAIGEEERCTLEDCRRMIAGVDKNGDGFVCFEDFAR 185
           R+ F +FD DGDG IT EEL SV  ++G ++  TLE C+ MI  VD++GDG V +++F +
Sbjct: 126 RDAFNVFDQDGDGFITVEELNSVMTSLGLKQGKTLECCKEMIMQVDEDGDGRVNYKEFLQ 185

[Close]

BLAST of MC00g0262 vs. TAIR 10
Match: AT1G66400.1 (calmodulin like 23 )

HSP 1 Score: 83.6 bits (205), Expect = 1.9e-16
Identity = 46/131 (35.11%), Postives = 76/131 (58.02%), Query Frame = 0


Query: 61  DMARHSRVSRKQLEALLGLSGADPPSHEEVAAMLSELDRHGDGVVSVEALMS-------H 120
           D     ++S  +L+ ++G    +  S EE  AM+ E D  G+G + ++  ++        
Sbjct: 24  DKNNDGKISIDELKDVIGALSPN-ASQEETKAMMKEFDLDGNGFIDLDEFVALFQISDQS 83

Query: 121 VGGACDEDLRETFEIFDADGDGRITAEELFSVFVAIGEEERCTLEDCRRMIAGVDKNGDG 180
              +   DL+E F+++D D +GRI+A EL SV   +G  E+C+++DC+RMI  VD +GDG
Sbjct: 84  SNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLG--EKCSIQDCQRMINKVDSDGDG 143

Query: 181 FVCFEDFARMM 185
            V FE+F +MM
Sbjct: 144 CVDFEEFKKMM 151

The following BLAST results are available for this feature:

BLAST of MC00g0262 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P30188	3.6e-33	46.83	Probable calcium-binding protein CML35 OS=Arabidopsis thaliana OX=3702 GN=CML35 ...	[more]
Q9SS31	3.1e-32	45.67	Probable calcium-binding protein CML36 OS=Arabidopsis thaliana OX=3702 GN=CML36 ...	[more]
Q8RZB5	5.1e-19	38.67	Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q0JC44	4.3e-18	41.18	Probable calcium-binding protein CML22 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q9FYK2	5.6e-18	37.98	Probable calcium-binding protein CML25 OS=Arabidopsis thaliana OX=3702 GN=CML25 ...	[more]

BLAST of MC00g0262 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022155928.1	7.15e-125	100.00	probable calcium-binding protein CML35 [Momordica charantia]	[more]
XP_038903370.1	1.05e-65	65.59	probable calcium-binding protein CML36 [Benincasa hispida]	[more]
XP_024181636.1	1.45e-63	58.18	probable calcium-binding protein CML36 [Rosa chinensis] >PRQ49697.1 putative EF-...	[more]
XP_008443339.2	9.28e-63	62.11	PREDICTED: probable calcium-binding protein CML35 [Cucumis melo] >KAA0053814.1 p...	[more]
XP_012085969.1	1.93e-61	57.94	probable calcium-binding protein CML35 [Jatropha curcas] >KDP26218.1 hypothetica...	[more]

BLAST of MC00g0262 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DP84	3.46e-125	100.00	probable calcium-binding protein CML35 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A2P6RTD5	7.02e-64	58.18	Putative EF-hand domain pair protein OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr2...	[more]
A0A5A7UEZ0	4.49e-63	62.11	Putative calcium-binding protein CML35 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A1S3B7R8	4.49e-63	62.11	probable calcium-binding protein CML35 OS=Cucumis melo OX=3656 GN=LOC103486950 P...	[more]
A0A067JTX4	9.33e-62	57.94	Uncharacterized protein OS=Jatropha curcas OX=180498 GN=JCGZ_22464 PE=4 SV=1	[more]

BLAST of MC00g0262 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G41410.1	2.6e-34	46.83	Calcium-binding EF-hand family protein	[more]
AT3G10190.1	2.2e-33	45.67	Calcium-binding EF-hand family protein	[more]
AT1G24620.1	4.0e-19	37.98	EF hand calcium-binding protein family	[more]
AT3G59440.1	1.1e-16	37.70	Calcium-binding EF-hand family protein	[more]
AT1G66400.1	1.9e-16	35.11	calmodulin like 23	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 89..117 e-value: 14.0 score: 8.6 coord: 121..149 e-value: 4.8E-5 score: 32.8 coord: 159..185 e-value: 3.2 score: 13.7
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 120..184 e-value: 1.6E-9 score: 38.0
IPR002048	EF-hand domain	PFAM	PF13833	EF-hand_8	coord: 73..108 e-value: 0.0028 score: 17.4
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 117..152 score: 14.5413
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 155..185 score: 12.309669
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 121..184 e-value: 1.39934E-17 score: 71.0397
None	No IPR available	GENE3D	1.10.238.10		coord: 59..185 e-value: 3.8E-24 score: 87.3
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 25..44
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..54
None	No IPR available	PANTHER	PTHR10891:SF790	CALCIUM-BINDING PROTEIN CML35-RELATED	coord: 4..185
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 4..185
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 130..142
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 168..180
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 61..184

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC00g0262.1	MC00g0262.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding