MC00g0056 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC00g0056
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionENTH domain-containing protein
Locationscaffold31: 1088030 .. 1090456 (+)
RNA-Seq ExpressionMC00g0056
SyntenyMC00g0056
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTCCAAGCAAGATTAGAAGGGCTTTAGGAGCAGTGAAGGACAAGACGAGCATAGGCCTTGCCAAAGTTGGCAGCAGCACCTCATTGTCGGATCTTGAAGTCGCGATTGTGAAGGCCACGAGACACGAAGAGTACCCAGCGGAGGAGCGCCACATTCGAGAGATTTTGAGCCTAACATGTTATTCAAGAGCGTATATAAGCGCGTGCGTCAACACCCTCTCGAGGAGGCTCAACAAGACCAAGAATTGGACCGTCGCGCTCAAGACGCTCATGCTGATCCAACGCTTGGTGTTGGAAGGTGACCCTGCGTACGAGCAAGAGATCTTCTTTTCAACCAGGCGTGGAACGAGGTTGTTAAACTTGATAGGTTATAGTATATTTAATCGTTTATTTATATTCTTTAGAGAGCAAGTGAATTTAAATGCCAGGTTAAACTAGCTACTATAGGATTGTGTCTTAAACTTTCAATGTTATGTCTAGTTGTTTTTTGAAATTTAAAATATATTTAGTAGGCACCTAACCACGCAATTTTGTATCTAATAATATATTATTTTTTAATATCTAATACCGTGACACATCAAATGAAAATTAAAAGTTTGGATATCTACTAAATACTTTTTAAGGTCTAGGGACTCATTTAAAATATTGCAAACTTCTACTAGACCCACTTATGTAATATAATTTTGAAAGTTCAACAACTAAACTTGTAATTTAACACCAAAAGTTTAAGCTTATAAAGTATGGTACTAGATACATTTAATCATTTACTCACATATTCTTTTAACACAGGTTCCTCAACATGTCAGATTTTAGAGACGTCTCTCAATCAGACTCTTGGGATTACTCGGCATTTGTTCGCACTTACGCATTGTACCTCGATGAAAGGCTCGAGTATCGCATGCAAAGTCGACGTGGGAAACGCAGCGCATTTGGAATAGAGGATGAAGAAGAACAAAACAATCAAAACAACATCAACAACATCGACACTAACAATAACAACAACAGCCAAGCCATTGTTGTAAGAGCCGCACCGGTACAAGAAATGACAACTGAACAAATATTCTCAAAGACACAGCATCTACAACAGCTTCTCGAACGCTTTTTGGCTTGTCGTCCTACAGGTTTGTATCCATATCCTCAAAATCATATACAAACGAAAATAAAGTCTCCTCTAAATTCATTTGTTTTTTCCTCCTTTTTTTTTCTTCTACAAACTAAAAGGCTGTTAAATTTTTATTGACCGATTAGCACCTAAGTATTTTGATCGAATCAAGTTGGCATGAAAATGAATGGATTATGTTCGGGTGTGGTTATTGGGCAGGCGCTGCAAAGAACAATAGAGTGGTGTTGGTGGCATTGTATCCAGTTGTGAAGGAAAGTTTCCAAATATATTATGACATAACTGAAATAATGGGGATTCTAATCGATCGTTTCATGGAGCTGAACATCCCCGATTGCGTCAAAGTGTACGAGATCTTTTGTCGTGTCGGGAAGCAGTTTGACGAGCTAGATCAACTGTATTCCTGGTGCAAAACCATTGGAATCGCTCGCTCTTCCGAGTACCCTGAAGTCGAGAAAATAACTCCAAAGAAACTCGAAGTGATGGACGATTTCATCAAAGACAAGTCCGCCTTGGCCCAGTGCAACAGGGACAGACCCAACAAGCCCCTCCAGCTCCAAATCACAGAACAAGAAGAAGAAGACTCAAAACAAGAACAAGAACCAGAAGATCAAGACATGAACGCCATTAAAGCTCTTCCCCCACCTGAGGATTTCAAGCCTGAACAAGAACCAGCAGCCATTGAAGTGAAGGAGGAGAGTGAACCAAAGGAAGACGAGAAGAAGAAGGATGAGAATCCAATGATGTGTGATCTGTTGAACTTGGGAGAAGATCATGCCATAATGAGAGAGGAGAATGCTGATAAATTGGCGTTGGCATTGTTCGATGGAAGTGGGCCAAAAGTTGAGGCTTTGCCAGCGCCAACATGGCAGGCCTTTAATGAGGAAACTTCAGATTGGGAGACTGCGTTGGTTCAATCAGCAAGCAATTTGAGCCATCAAAGGGCTGAACTTGGGGGTGGATTCGACATGCTGCTTCTGGATGGGATGTATAAGCAGCCCACAGTAGTCACAGGAGTGGGCAGCTCAGGGAGTGCCAGCAGCATGGCTCTTGGGTCTGCAGGAAGGCCTGCCATGCTTGCCTTGCCGGCCCCTCCGCCTCCGGGAGAAGGCGGCGCTTCGAGTTCCTCGAGCGACCCTTTTGCGGCATCGGTGGCGGTGGCGCCGCCATCGTACGTCCAGATGTCGGAAATGGAGAGGAAGCAGAAGCTGTTGGTGGAGGAGCAGCTGATGTGGCAGCAATATGCGAGGGATGGGAGGCAAATACAGCACAACCCTTATGCAGGAGGATTCACACACAGTTAT

mRNA sequence

ATGGCTCCAAGCAAGATTAGAAGGGCTTTAGGAGCAGTGAAGGACAAGACGAGCATAGGCCTTGCCAAAGTTGGCAGCAGCACCTCATTGTCGGATCTTGAAGTCGCGATTGTGAAGGCCACGAGACACGAAGAGTACCCAGCGGAGGAGCGCCACATTCGAGAGATTTTGAGCCTAACATGTTATTCAAGAGCGTATATAAGCGCGTGCGTCAACACCCTCTCGAGGAGGCTCAACAAGACCAAGAATTGGACCGTCGCGCTCAAGACGCTCATGCTGATCCAACGCTTGGTGTTGGAAGGTGACCCTGCGTACGAGCAAGAGATCTTCTTTTCAACCAGGCGTGGAACGAGGTTCCTCAACATGTCAGATTTTAGAGACGTCTCTCAATCAGACTCTTGGGATTACTCGGCATTTGTTCGCACTTACGCATTGTACCTCGATGAAAGGCTCGAGTATCGCATGCAAAGTCGACGTGGGAAACGCAGCGCATTTGGAATAGAGGATGAAGAAGAACAAAACAATCAAAACAACATCAACAACATCGACACTAACAATAACAACAACAGCCAAGCCATTGTTGTAAGAGCCGCACCGGTACAAGAAATGACAACTGAACAAATATTCTCAAAGACACAGCATCTACAACAGCTTCTCGAACGCTTTTTGGCTTGTCGTCCTACAGGCGCTGCAAAGAACAATAGAGTGGTGTTGGTGGCATTGTATCCAGTTGTGAAGGAAAGTTTCCAAATATATTATGACATAACTGAAATAATGGGGATTCTAATCGATCGTTTCATGGAGCTGAACATCCCCGATTGCGTCAAAGTGTACGAGATCTTTTGTCGTGTCGGGAAGCAGTTTGACGAGCTAGATCAACTGTATTCCTGGTGCAAAACCATTGGAATCGCTCGCTCTTCCGAGTACCCTGAAGTCGAGAAAATAACTCCAAAGAAACTCGAAGTGATGGACGATTTCATCAAAGACAAGTCCGCCTTGGCCCAGTGCAACAGGGACAGACCCAACAAGCCCCTCCAGCTCCAAATCACAGAACAAGAAGAAGAAGACTCAAAACAAGAACAAGAACCAGAAGATCAAGACATGAACGCCATTAAAGCTCTTCCCCCACCTGAGGATTTCAAGCCTGAACAAGAACCAGCAGCCATTGAAGTGAAGGAGGAGAGTGAACCAAAGGAAGACGAGAAGAAGAAGGATGAGAATCCAATGATGTGTGATCTGTTGAACTTGGGAGAAGATCATGCCATAATGAGAGAGGAGAATGCTGATAAATTGGCGTTGGCATTGTTCGATGGAAGTGGGCCAAAAGTTGAGGCTTTGCCAGCGCCAACATGGCAGGCCTTTAATGAGGAAACTTCAGATTGGGAGACTGCGTTGGTTCAATCAGCAAGCAATTTGAGCCATCAAAGGGCTGAACTTGGGGGTGGATTCGACATGCTGCTTCTGGATGGGATGTATAAGCAGCCCACAGTAGTCACAGGAGTGGGCAGCTCAGGGAGTGCCAGCAGCATGGCTCTTGGGTCTGCAGGAAGGCCTGCCATGCTTGCCTTGCCGGCCCCTCCGCCTCCGGGAGAAGGCGGCGCTTCGAGTTCCTCGAGCGACCCTTTTGCGGCATCGGTGGCGGTGGCGCCGCCATCGTACGTCCAGATGTCGGAAATGGAGAGGAAGCAGAAGCTGTTGGTGGAGGAGCAGCTGATGTGGCAGCAATATGCGAGGGATGGGAGGCAAATACAGCACAACCCTTATGCAGGAGGATTCACACACAGTTAT

Coding sequence (CDS)

ATGGCTCCAAGCAAGATTAGAAGGGCTTTAGGAGCAGTGAAGGACAAGACGAGCATAGGCCTTGCCAAAGTTGGCAGCAGCACCTCATTGTCGGATCTTGAAGTCGCGATTGTGAAGGCCACGAGACACGAAGAGTACCCAGCGGAGGAGCGCCACATTCGAGAGATTTTGAGCCTAACATGTTATTCAAGAGCGTATATAAGCGCGTGCGTCAACACCCTCTCGAGGAGGCTCAACAAGACCAAGAATTGGACCGTCGCGCTCAAGACGCTCATGCTGATCCAACGCTTGGTGTTGGAAGGTGACCCTGCGTACGAGCAAGAGATCTTCTTTTCAACCAGGCGTGGAACGAGGTTCCTCAACATGTCAGATTTTAGAGACGTCTCTCAATCAGACTCTTGGGATTACTCGGCATTTGTTCGCACTTACGCATTGTACCTCGATGAAAGGCTCGAGTATCGCATGCAAAGTCGACGTGGGAAACGCAGCGCATTTGGAATAGAGGATGAAGAAGAACAAAACAATCAAAACAACATCAACAACATCGACACTAACAATAACAACAACAGCCAAGCCATTGTTGTAAGAGCCGCACCGGTACAAGAAATGACAACTGAACAAATATTCTCAAAGACACAGCATCTACAACAGCTTCTCGAACGCTTTTTGGCTTGTCGTCCTACAGGCGCTGCAAAGAACAATAGAGTGGTGTTGGTGGCATTGTATCCAGTTGTGAAGGAAAGTTTCCAAATATATTATGACATAACTGAAATAATGGGGATTCTAATCGATCGTTTCATGGAGCTGAACATCCCCGATTGCGTCAAAGTGTACGAGATCTTTTGTCGTGTCGGGAAGCAGTTTGACGAGCTAGATCAACTGTATTCCTGGTGCAAAACCATTGGAATCGCTCGCTCTTCCGAGTACCCTGAAGTCGAGAAAATAACTCCAAAGAAACTCGAAGTGATGGACGATTTCATCAAAGACAAGTCCGCCTTGGCCCAGTGCAACAGGGACAGACCCAACAAGCCCCTCCAGCTCCAAATCACAGAACAAGAAGAAGAAGACTCAAAACAAGAACAAGAACCAGAAGATCAAGACATGAACGCCATTAAAGCTCTTCCCCCACCTGAGGATTTCAAGCCTGAACAAGAACCAGCAGCCATTGAAGTGAAGGAGGAGAGTGAACCAAAGGAAGACGAGAAGAAGAAGGATGAGAATCCAATGATGTGTGATCTGTTGAACTTGGGAGAAGATCATGCCATAATGAGAGAGGAGAATGCTGATAAATTGGCGTTGGCATTGTTCGATGGAAGTGGGCCAAAAGTTGAGGCTTTGCCAGCGCCAACATGGCAGGCCTTTAATGAGGAAACTTCAGATTGGGAGACTGCGTTGGTTCAATCAGCAAGCAATTTGAGCCATCAAAGGGCTGAACTTGGGGGTGGATTCGACATGCTGCTTCTGGATGGGATGTATAAGCAGCCCACAGTAGTCACAGGAGTGGGCAGCTCAGGGAGTGCCAGCAGCATGGCTCTTGGGTCTGCAGGAAGGCCTGCCATGCTTGCCTTGCCGGCCCCTCCGCCTCCGGGAGAAGGCGGCGCTTCGAGTTCCTCGAGCGACCCTTTTGCGGCATCGGTGGCGGTGGCGCCGCCATCGTACGTCCAGATGTCGGAAATGGAGAGGAAGCAGAAGCTGTTGGTGGAGGAGCAGCTGATGTGGCAGCAATATGCGAGGGATGGGAGGCAAATACAGCACAACCCTTATGCAGGAGGATTCACACACAGTTAT

Protein sequence

MAPSKIRRALGAVKDKTSIGLAKVGSSTSLSDLEVAIVKATRHEEYPAEERHIREILSLTCYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVLEGDPAYEQEIFFSTRRGTRFLNMSDFRDVSQSDSWDYSAFVRTYALYLDERLEYRMQSRRGKRSAFGIEDEEEQNNQNNINNIDTNNNNNSQAIVVRAAPVQEMTTEQIFSKTQHLQQLLERFLACRPTGAAKNNRVVLVALYPVVKESFQIYYDITEIMGILIDRFMELNIPDCVKVYEIFCRVGKQFDELDQLYSWCKTIGIARSSEYPEVEKITPKKLEVMDDFIKDKSALAQCNRDRPNKPLQLQITEQEEEDSKQEQEPEDQDMNAIKALPPPEDFKPEQEPAAIEVKEESEPKEDEKKKDENPMMCDLLNLGEDHAIMREENADKLALALFDGSGPKVEALPAPTWQAFNEETSDWETALVQSASNLSHQRAELGGGFDMLLLDGMYKQPTVVTGVGSSGSASSMALGSAGRPAMLALPAPPPPGEGGASSSSSDPFAASVAVAPPSYVQMSEMERKQKLLVEEQLMWQQYARDGRQIQHNPYAGGFTHSY
Homology
BLAST of MC00g0056 vs. ExPASy Swiss-Prot
Match: Q9SA65 (Putative clathrin assembly protein At1g03050 OS=Arabidopsis thaliana OX=3702 GN=At1g03050 PE=2 SV=1)

HSP 1 Score: 667.9 bits (1722), Expect = 1.0e-190
Identity = 386/604 (63.91%), Postives = 470/604 (77.81%), Query Frame = 0

Query: 1   MAPSKIRRALGAVKDKTSIGLAKV-GSSTSLSDLEVAIVKATRHEEYPAEERHIREILSL 60
           M  SK +RA+GAVKD+TS+GLAKV G S SLS+L+VAIVKATRHEE+PAEE++IREILSL
Sbjct: 1   MGSSKFKRAIGAVKDQTSVGLAKVNGRSASLSELDVAIVKATRHEEFPAEEKYIREILSL 60

Query: 61  TCYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVLEGDPAYEQEIFFSTRRGTRF 120
           T YSR+YI+ACV+TLSRRLNKTK WTVALKTL+LIQRL+ EGD AYEQEIFF+TRRGTR 
Sbjct: 61  TSYSRSYINACVSTLSRRLNKTKCWTVALKTLILIQRLLGEGDQAYEQEIFFATRRGTRL 120

Query: 121 LNMSDFRDVSQSDSWDYSAFVRTYALYLDERLEYRMQSRRGKRSAF---GIEDEEEQNNQ 180
           LNMSDFRDVS+S+SWDYSAFVRTYALYLDERL++RMQ+R GKR  +   G  DEEEQ   
Sbjct: 121 LNMSDFRDVSRSNSWDYSAFVRTYALYLDERLDFRMQARHGKRGVYCVGGEADEEEQ--- 180

Query: 181 NNINNIDTNNNNNSQAIVVRAAPVQEMTTEQIFSKTQHLQQLLERFLACRPTGAAKNNRV 240
                 D    + S AIVVR+ P+ EM TEQIF + QHLQQLL+RFLACRPTG A+NNRV
Sbjct: 181 ------DQAAADLSTAIVVRSQPIAEMKTEQIFIRIQHLQQLLDRFLACRPTGNARNNRV 240

Query: 241 VLVALYPVVKESFQIYYDITEIMGILIDRFMELNIPDCVKVYEIFCRVGKQFDELDQLYS 300
           V+VALYP+VKESFQIYYD+TEIMGILI+RFMEL+IPD +KVY+IFCRV KQF+ELDQ YS
Sbjct: 241 VIVALYPIVKESFQIYYDVTEIMGILIERFMELDIPDSIKVYDIFCRVSKQFEELDQFYS 300

Query: 301 WCKTIGIARSSEYPEVEKITPKKLEVMDDFIKDKSALAQCNRDRPNKPLQLQITEQEEED 360
           WCK +GIARSSEYPE+EKIT KKL++MD+FI+DKSAL      + +K ++ +  E ++E 
Sbjct: 301 WCKNMGIARSSEYPEIEKITQKKLDLMDEFIRDKSAL---EHTKQSKSVKSEADEDDDEA 360

Query: 361 SKQEQEPEDQDMNAIKALPPPEDFKPEQEPAAIEVKEESEPKED---EKKKDENPMMCDL 420
             +E   E +DMNAIKALP P    P++E    +VK E E KE+   EKK++E   M DL
Sbjct: 361 RTEEVNEEQEDMNAIKALPEP---PPKEED---DVKPEEEAKEEVIIEKKQEE---MGDL 420

Query: 421 LNLGEDHAIMREENADKLALALFDG---SGPKVEALPAPTWQAFNEETSDWETALVQSAS 480
           L+LG  +     +  D LALALFDG   SG   E+   P W+AF ++++DWETALVQ+A+
Sbjct: 421 LDLGNTNGGEAGQAGDSLALALFDGPYASGSGSES--GPGWEAFKDDSADWETALVQTAT 480

Query: 481 NLSHQRAELGGGFDMLLLDGMYKQPTV------VTGVGSSGSASSMALGSAGRPA--MLA 540
           NLS Q++ELGGGFDMLLL+GMY+   V       T  G+SGSASSMA GSAGRPA  MLA
Sbjct: 481 NLSGQKSELGGGFDMLLLNGMYQHGAVNAAVKTSTAYGASGSASSMAFGSAGRPAATMLA 540

Query: 541 LPAPPPPGEGGASSSSS----DPFAASVAVAPPSYVQMSEMERKQKLLVEEQLMWQQYAR 583
           LPA P    G A + +S    DPFAAS+ VAPP+YVQM++ME+KQ++L+EEQ+MW QY+R
Sbjct: 541 LPA-PSTANGNAGNINSPVPMDPFAASLEVAPPAYVQMNDMEKKQRMLMEEQMMWDQYSR 580

BLAST of MC00g0056 vs. ExPASy Swiss-Prot
Match: Q8GX47 (Putative clathrin assembly protein At4g02650 OS=Arabidopsis thaliana OX=3702 GN=At4g02650 PE=2 SV=2)

HSP 1 Score: 650.2 bits (1676), Expect = 2.3e-185
Identity = 366/593 (61.72%), Postives = 460/593 (77.57%), Query Frame = 0

Query: 1   MAPSKIRRALGAVKDKTSIGLAKVGS-STSLSDLEVAIVKATRHEEYPAEERHIREILSL 60
           M  SK++RA+GAVKD+TS+GLAKVG  S+SL++LE+A+VKATRH++YPAE+++IREIL L
Sbjct: 1   MGSSKLKRAIGAVKDQTSVGLAKVGGRSSSLTELEIAVVKATRHDDYPAEDKYIREILCL 60

Query: 61  TCYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVLEGDPAYEQEIFFSTRRGTRF 120
           T YSR Y+SACV TLSRRLNKTKNW+VALKTL+LIQRL+ +GD AYEQEIFF+TRRGTR 
Sbjct: 61  TSYSRNYVSACVATLSRRLNKTKNWSVALKTLILIQRLLTDGDRAYEQEIFFATRRGTRL 120

Query: 121 LNMSDFRDVSQSDSWDYSAFVRTYALYLDERLEYRMQSRRGKRSAFGIEDEEEQNNQNNI 180
           LNMSDFRD SQSDSWDYSAFVRTYALYLDERL+YRMQ RRGK+ + G    +  + + + 
Sbjct: 121 LNMSDFRDASQSDSWDYSAFVRTYALYLDERLDYRMQGRRGKKKSGGGGGGDGDSGEED- 180

Query: 181 NNIDTNNNNNSQAIVVRAAPVQEMTTEQIFSKTQHLQQLLERFLACRPTGAAKNNRVVLV 240
           ++  T+N+  S+AIVV++ PV EM TE+IF++ QHLQQLL+RFLACRPTG AKNNRVV+V
Sbjct: 181 DHRGTSNDIRSKAIVVKSKPVAEMKTEKIFNRVQHLQQLLDRFLACRPTGNAKNNRVVIV 240

Query: 241 ALYPVVKESFQIYYDITEIMGILIDRFMELNIPDCVKVYEIFCRVGKQFDELDQLYSWCK 300
           A+YP+VKESFQ+YY+ITEIMG+LI+RFMEL+I D +KVYEIFCRV KQFDELD  Y WCK
Sbjct: 241 AMYPIVKESFQLYYNITEIMGVLIERFMELDIHDSIKVYEIFCRVSKQFDELDPFYGWCK 300

Query: 301 TIGIARSSEYPEVEKITPKKLEVMDDFIKDKSALAQCNRDRPNKPLQLQITEQEEEDSKQ 360
            + +ARSSEYPE+EKIT KKL++MD+FI+DKSALA     +  K    +  + EEE+SK 
Sbjct: 301 NMAVARSSEYPELEKITQKKLDLMDEFIRDKSALAA----QTTKSSSKRSNKSEEEESKT 360

Query: 361 EQEPEDQ-DMNAIKALPPPEDFKPEQEPAAIEVKEESEPKEDEKKKDENPMMCDLLNLGE 420
           E   E+Q D+N+IKALP PE  + E+E   +E K++ E  E   ++D+     DLL+L +
Sbjct: 361 EYIQENQEDLNSIKALPAPEQKEEEEEEEKMETKKDVE--EVVSRQDQEG---DLLDLTD 420

Query: 421 DHAIMREENADKLALALFDGSGPKVEALPAPTWQAFNEETSDWETALVQSASNLSHQRAE 480
           +  +      D LALALFDG     E+   P W+AFN+ ++DWET LV+SA+ LS Q++E
Sbjct: 421 EAGVTAGTVGDSLALALFDGV-VGTESASGPGWEAFNDNSADWETDLVRSATRLSGQKSE 480

Query: 481 LGGGFDMLLLDGMYKQPTV------VTGVGSSGSASSMALGSAGRPA--MLALPAPPPPG 540
           LGGGFD LLLDGMY+   V       T  GSSGSASS+A GSAG PA  MLALPAPPP  
Sbjct: 481 LGGGFDTLLLDGMYQYGAVNAAVKTSTAYGSSGSASSVAFGSAGSPAASMLALPAPPPTA 540

Query: 541 EGGASSS-SSDPFAASVAVAPPSYVQMSEMERKQKLLVEEQLMWQQYARDGRQ 583
            G  +S    DPFAAS+ VAPP+YVQM++ME+KQ+LL+EEQ+MW QY R GRQ
Sbjct: 541 NGNRNSPVMVDPFAASLEVAPPAYVQMNDMEKKQRLLMEEQIMWDQYNRSGRQ 582

BLAST of MC00g0056 vs. ExPASy Swiss-Prot
Match: Q8LF20 (Putative clathrin assembly protein At2g25430 OS=Arabidopsis thaliana OX=3702 GN=At2g25430 PE=1 SV=2)

HSP 1 Score: 460.3 bits (1183), Expect = 3.3e-128
Identity = 297/652 (45.55%), Postives = 399/652 (61.20%), Query Frame = 0

Query: 1   MAPSKIRRALGAVKDKTSIGLAKVGSSTSLSDLEVAIVKATRHEEYPAEERHIREILSLT 60
           MAPS IR+A+GAVKD+TSIG+AKV S+ +  DLEVAIVKAT H++ PA E++IREIL+LT
Sbjct: 1   MAPS-IRKAIGAVKDQTSIGIAKVASNMA-PDLEVAIVKATSHDDDPASEKYIREILNLT 60

Query: 61  CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVLEGDPAYEQEIFFSTRRGTRFL 120
             SR YI ACV ++SRRL+KT++W VALK LML+ RL+ EGDP +++EI +STRRGTR L
Sbjct: 61  SLSRGYILACVTSVSRRLSKTRDWVVALKALMLVHRLLNEGDPIFQEEILYSTRRGTRML 120

Query: 121 NMSDFRDVSQSDSWDYSAFVRTYALYLDERLEYRMQSRRGKRSAFGIEDEEEQNNQNNIN 180
           NMSDFRD + S SWD+SAFVRTYA YLD+RLE  +  R+      G+      N+ ++ N
Sbjct: 121 NMSDFRDEAHSSSWDHSAFVRTYAGYLDQRLELALFERKS-----GVSVNSGGNSSHHSN 180

Query: 181 NID-----------------------------TNNNNNSQAIVVRA-------------- 240
           N D                               +NN    +  R+              
Sbjct: 181 NDDRYGRGRDDFRSPPPRSYDYENGGGGGSDFRGDNNGYGGVPKRSRSYGDMTEMGGGGG 240

Query: 241 ----------APVQEMTTEQIFSKTQHLQQLLERFLACRPTGAAKNNRVVLVALYPVVKE 300
                      P++EMT E+IF K  HLQ+LL+RFL+ RPTG AKN+R++L+ALYPVV+E
Sbjct: 241 GGGRDEKKVVTPLREMTPERIFGKMGHLQRLLDRFLSLRPTGLAKNSRMILIALYPVVRE 300

Query: 301 SFQIYYDITEIMGILIDRFMELNIPDCVKVYEIFCRVGKQFDELDQLYSWCKTIGIARSS 360
           SF++Y DI E++ +L+D+F ++   DCVK ++ +    KQ DEL   Y+WCK  G+ARSS
Sbjct: 301 SFKLYADICEVLAVLLDKFFDMEYSDCVKAFDAYASAAKQIDELIAFYNWCKETGVARSS 360

Query: 361 EYPEVEKITPKKLEVMDDFIKDKSALAQCNRDRPNKPLQLQITEQEEEDSKQEQEPEDQD 420
           EYPEV++IT K LE +++F++D++        R   P + +I        ++E EP   D
Sbjct: 361 EYPEVQRITSKLLETLEEFVRDRA-------KRGKSPERKEIEAPPPVVEEEEPEP---D 420

Query: 421 MNAIKALPPPEDFKPEQEPAAIEVKEESEPKEDEKKKDENPMMCDLLNLGEDHAIMREEN 480
           MN IKALPPPE++ P   P       E EP+ ++ +  E     DL+NL ED  +  ++ 
Sbjct: 421 MNEIKALPPPENYTPPPPP-------EPEPQPEKPQFTE-----DLVNLREDE-VTADDQ 480

Query: 481 ADKLALALF---DGSGPKVEALPA----PTWQ--AFNEETSDWETALVQSASNLSHQRAE 540
            +K ALALF    G+  K EA  +      WQ  A     +DWE ALV++ SNL  Q A 
Sbjct: 481 GNKFALALFAGPPGNNGKWEAFSSNGVTSAWQNPAAEPGKADWELALVETTSNLEKQTAA 540

Query: 541 LGGGFDMLLLDGMYKQPTVVTGVGSS----GSASSMALGSAGRP--AMLALPAPPPPGEG 585
           LGGGFD LLL+GMY Q  V   V +S    GSASS+AL   G+    +LALPAP    +G
Sbjct: 541 LGGGFDNLLLNGMYDQGMVRQHVSTSQLTGGSASSVALPLPGKTNNQVLALPAP----DG 600

BLAST of MC00g0056 vs. ExPASy Swiss-Prot
Match: Q8S9J8 (Probable clathrin assembly protein At4g32285 OS=Arabidopsis thaliana OX=3702 GN=At4g32285 PE=1 SV=2)

HSP 1 Score: 453.4 bits (1165), Expect = 4.1e-126
Identity = 284/624 (45.51%), Postives = 389/624 (62.34%), Query Frame = 0

Query: 6   IRRALGAVKDKTSIGLAKVGSSTSLSDLEVAIVKATRHEEYPAEERHIREILSLTCYSRA 65
           +R+A+G VKD+TSIG+AKV S+ +  DLEVAIVKAT H++  + +++IREILSLT  SR 
Sbjct: 5   MRKAIGVVKDQTSIGIAKVASNMA-PDLEVAIVKATSHDDDQSSDKYIREILSLTSLSRG 64

Query: 66  YISACVNTLSRRLNKTKNWTVALKTLMLIQRLVLEGDPAYEQEIFFSTRRGTRFLNMSDF 125
           Y+ ACV ++SRRL KT++W VALK LML+ RL+ EGDP +++EI ++TRRGTR LNMSDF
Sbjct: 65  YVHACVTSVSRRLKKTRDWIVALKALMLVHRLLNEGDPLFQEEILYATRRGTRILNMSDF 124

Query: 126 RDVSQSDSWDYSAFVRTYALYLDERLEYRMQSRRGKRSAFGIEDEEEQNNQNNIN----- 185
           RD + S SWD+SAFVRTYA YLD+RLE  +  RRG R+  G     + N  +  N     
Sbjct: 125 RDEAHSSSWDHSAFVRTYASYLDQRLELALFERRG-RNGGGSSSSHQSNGDDGYNRSRDD 184

Query: 186 ---------NIDTNNN----------NNSQAIVVR-----AAPVQEMTTEQIFSKTQHLQ 245
                    + +T N            +   I  R       P++EMT E+IF K  HLQ
Sbjct: 185 FRSPPPRTYDYETGNGFGMPKRSRSFGDVNEIGAREEKKSVTPLREMTPERIFGKMGHLQ 244

Query: 246 QLLERFLACRPTGAAKNNRVVLVALYPVVKESFQIYYDITEIMGILIDRFMELNIPDCVK 305
           +LL+RFL+CRPTG AKN+R++L+A+YPVVKESF++Y DI E++ +L+D+F ++   DCVK
Sbjct: 245 RLLDRFLSCRPTGLAKNSRMILIAMYPVVKESFRLYADICEVLAVLLDKFFDMEYTDCVK 304

Query: 306 VYEIFCRVGKQFDELDQLYSWCKTIGIARSSEYPEVEKITPKKLEVMDDFIKDKSALAQC 365
            ++ +    KQ DEL   Y WCK  G+ARSSEYPEV++IT K LE +++F++D++  A+ 
Sbjct: 305 AFDAYASAAKQIDELIAFYHWCKDTGVARSSEYPEVQRITSKLLETLEEFVRDRAKRAKS 364

Query: 366 NRDRPNKPLQLQITEQEEEDSKQEQEPEDQDMNAIKALPPPEDFKPEQEPAAIEVKEESE 425
              +          E E   +      E  DMN IKALPPPE+  P   PA        E
Sbjct: 365 PERK----------EIEAPPAPAPPVEEPVDMNEIKALPPPENHTPPPPPA-------PE 424

Query: 426 PKEDEKKKDENPMMCDLLNLGEDHAIMREENADKLALALFDG---SGPKVEALPA----- 485
           PK  + +  +     DL+NL ED  +  ++  +K ALALF G   +  K EA  +     
Sbjct: 425 PKPQQPQVTD-----DLVNLREDD-VSGDDQGNKFALALFAGPPANNGKWEAFSSDNNVT 484

Query: 486 PTWQ--AFNEETSDWETALVQSASNLSHQRAELGGGFDMLLLDGMYKQPTVVTGVGSS-- 545
             WQ  A     +DWE ALV++ASNL HQ+A +GGG D LLL+GMY Q  V   V +S  
Sbjct: 485 SAWQNPAAELGKADWELALVETASNLEHQKAAMGGGLDPLLLNGMYDQGAVRQHVSTSEL 544

Query: 546 --GSASSMALGSAGR--PAMLALPAPPPPGEGGASSSSSDPFAASVAVAPPSYVQMSEME 585
             GS+SS+AL   G+    +LALPAP    +G     + DPFAAS+ + PPSYVQM+EM+
Sbjct: 545 TGGSSSSVALPLPGKVNSHILALPAP----DGTVQKVNQDPFAASLTIPPPSYVQMAEMD 599

BLAST of MC00g0056 vs. ExPASy Swiss-Prot
Match: Q9ZVN6 (Clathrin coat assembly protein AP180 OS=Arabidopsis thaliana OX=3702 GN=AP180 PE=1 SV=1)

HSP 1 Score: 254.2 bits (648), Expect = 3.6e-66
Identity = 136/335 (40.60%), Postives = 215/335 (64.18%), Query Frame = 0

Query: 3   PSKIRRALGAVKDKTSIGLAKVGSSTS----LSDLEVAIVKATRH-EEYPAEERHIREIL 62
           PSK+++A+GAVKD+TSI LAKV +  +    L+ LEVAI+KAT H EE P ++R + EIL
Sbjct: 2   PSKLKKAIGAVKDQTSISLAKVANGATGGGDLTTLEVAILKATSHDEEVPIDDRLVTEIL 61

Query: 63  SLTCYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVLEGDPAYEQEIFFSTRRGT 122
            +    +++ ++C   + RR+ +T+NW VALK+L+L+ R+  +GDP + +E+  + +RG 
Sbjct: 62  GIISSKKSHAASCAAAIGRRIGRTRNWIVALKSLVLVLRIFQDGDPYFPREVLHAMKRGA 121

Query: 123 RFLNMSDFRDVSQSDSWDYSAFVRTYALYLDERLEYRMQSRRGKRSAFGIEDEEEQNNQN 182
           + LN+S FRD S S  WD++AFVRT+ALYLDERL+  +  +  +R         EQ  + 
Sbjct: 122 KILNLSSFRDDSNSCPWDFTAFVRTFALYLDERLDCFLTGKLQRRYT-----NREQTGRI 181

Query: 183 NINNIDTNNNNNSQAIVVRAAPVQEMTTEQIFSKTQHLQQLLERFLACRPTGAAKNNRVV 242
           + N+   +  N    I      V++M    +  K  + Q+LL+R +A RPTG AK NR+V
Sbjct: 182 STNSTTRSRFNPKAGIKSHEPAVRDMKPVMLLDKITYWQKLLDRAIATRPTGDAKANRLV 241

Query: 243 LVALYPVVKESFQIYYDITEIMGILIDRFMELNIPDCVKVYEIFCRVGKQFDELDQLYSW 302
            ++LY V++ESF +Y DI++ + +L+D F  L    C+  ++   R  KQF+EL+  Y  
Sbjct: 242 KMSLYAVMQESFDLYRDISDGLALLLDSFFHLQYQSCINAFQACVRASKQFEELNAFYDL 301

Query: 303 CKTIGIARSSEYPEVEKITPKKLEVMDDFIKDKSA 333
            K+IGI R+SEYP ++KI+ + LE + +F+KD+S+
Sbjct: 302 SKSIGIGRTSEYPSIQKISLELLETLQEFLKDQSS 331

BLAST of MC00g0056 vs. NCBI nr
Match: XP_022154412.1 (putative clathrin assembly protein At1g03050 [Momordica charantia])

HSP 1 Score: 1147 bits (2966), Expect = 0.0
Identity = 596/596 (100.00%), Postives = 596/596 (100.00%), Query Frame = 0

Query: 1   MAPSKIRRALGAVKDKTSIGLAKVGSSTSLSDLEVAIVKATRHEEYPAEERHIREILSLT 60
           MAPSKIRRALGAVKDKTSIGLAKVGSSTSLSDLEVAIVKATRHEEYPAEERHIREILSLT
Sbjct: 1   MAPSKIRRALGAVKDKTSIGLAKVGSSTSLSDLEVAIVKATRHEEYPAEERHIREILSLT 60

Query: 61  CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVLEGDPAYEQEIFFSTRRGTRFL 120
           CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVLEGDPAYEQEIFFSTRRGTRFL
Sbjct: 61  CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVLEGDPAYEQEIFFSTRRGTRFL 120

Query: 121 NMSDFRDVSQSDSWDYSAFVRTYALYLDERLEYRMQSRRGKRSAFGIEDEEEQNNQNNIN 180
           NMSDFRDVSQSDSWDYSAFVRTYALYLDERLEYRMQSRRGKRSAFGIEDEEEQNNQNNIN
Sbjct: 121 NMSDFRDVSQSDSWDYSAFVRTYALYLDERLEYRMQSRRGKRSAFGIEDEEEQNNQNNIN 180

Query: 181 NIDTNNNNNSQAIVVRAAPVQEMTTEQIFSKTQHLQQLLERFLACRPTGAAKNNRVVLVA 240
           NIDTNNNNNSQAIVVRAAPVQEMTTEQIFSKTQHLQQLLERFLACRPTGAAKNNRVVLVA
Sbjct: 181 NIDTNNNNNSQAIVVRAAPVQEMTTEQIFSKTQHLQQLLERFLACRPTGAAKNNRVVLVA 240

Query: 241 LYPVVKESFQIYYDITEIMGILIDRFMELNIPDCVKVYEIFCRVGKQFDELDQLYSWCKT 300
           LYPVVKESFQIYYDITEIMGILIDRFMELNIPDCVKVYEIFCRVGKQFDELDQLYSWCKT
Sbjct: 241 LYPVVKESFQIYYDITEIMGILIDRFMELNIPDCVKVYEIFCRVGKQFDELDQLYSWCKT 300

Query: 301 IGIARSSEYPEVEKITPKKLEVMDDFIKDKSALAQCNRDRPNKPLQLQITEQEEEDSKQE 360
           IGIARSSEYPEVEKITPKKLEVMDDFIKDKSALAQCNRDRPNKPLQLQITEQEEEDSKQE
Sbjct: 301 IGIARSSEYPEVEKITPKKLEVMDDFIKDKSALAQCNRDRPNKPLQLQITEQEEEDSKQE 360

Query: 361 QEPEDQDMNAIKALPPPEDFKPEQEPAAIEVKEESEPKEDEKKKDENPMMCDLLNLGEDH 420
           QEPEDQDMNAIKALPPPEDFKPEQEPAAIEVKEESEPKEDEKKKDENPMMCDLLNLGEDH
Sbjct: 361 QEPEDQDMNAIKALPPPEDFKPEQEPAAIEVKEESEPKEDEKKKDENPMMCDLLNLGEDH 420

Query: 421 AIMREENADKLALALFDGSGPKVEALPAPTWQAFNEETSDWETALVQSASNLSHQRAELG 480
           AIMREENADKLALALFDGSGPKVEALPAPTWQAFNEETSDWETALVQSASNLSHQRAELG
Sbjct: 421 AIMREENADKLALALFDGSGPKVEALPAPTWQAFNEETSDWETALVQSASNLSHQRAELG 480

Query: 481 GGFDMLLLDGMYKQPTVVTGVGSSGSASSMALGSAGRPAMLALPAPPPPGEGGASSSSSD 540
           GGFDMLLLDGMYKQPTVVTGVGSSGSASSMALGSAGRPAMLALPAPPPPGEGGASSSSSD
Sbjct: 481 GGFDMLLLDGMYKQPTVVTGVGSSGSASSMALGSAGRPAMLALPAPPPPGEGGASSSSSD 540

Query: 541 PFAASVAVAPPSYVQMSEMERKQKLLVEEQLMWQQYARDGRQIQHNPYAGGFTHSY 596
           PFAASVAVAPPSYVQMSEMERKQKLLVEEQLMWQQYARDGRQIQHNPYAGGFTHSY
Sbjct: 541 PFAASVAVAPPSYVQMSEMERKQKLLVEEQLMWQQYARDGRQIQHNPYAGGFTHSY 596

BLAST of MC00g0056 vs. NCBI nr
Match: XP_038888902.1 (putative clathrin assembly protein At1g03050 isoform X1 [Benincasa hispida])

HSP 1 Score: 910 bits (2353), Expect = 0.0
Identity = 494/617 (80.06%), Postives = 542/617 (87.84%), Query Frame = 0

Query: 1   MAPSKIRRALGAVKDKTSIGLAKVGSSTSLSDLEVAIVKATRHEEYPAEERHIREILSLT 60
           MAPSKIRRALGAVKDKTSIGLAKVGSSTSLSDLEVAIVKATRHEEYPAEERH+REILSLT
Sbjct: 1   MAPSKIRRALGAVKDKTSIGLAKVGSSTSLSDLEVAIVKATRHEEYPAEERHVREILSLT 60

Query: 61  CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVLEGDPAYEQEIFFSTRRGTRFL 120
           CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLV EGDPAYEQEIFFSTRRGTRFL
Sbjct: 61  CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVSEGDPAYEQEIFFSTRRGTRFL 120

Query: 121 NMSDFRDVSQSDSWDYSAFVRTYALYLDERLEYRMQSRRGKRSAFGIEDEEEQ------- 180
           NMSDFRD SQS+SWDYSAFVRT+ALYLDERLE+RMQSRRGKRSAFG++DEE Q       
Sbjct: 121 NMSDFRDTSQSNSWDYSAFVRTFALYLDERLEFRMQSRRGKRSAFGLDDEEGQQQQINNN 180

Query: 181 NNQNNINNIDTNNNN---NSQAIVVRAAPVQEMTTEQIFSKTQHLQQLLERFLACRPTGA 240
           NN NN N  D +NN+   NS+AIVVRA P+++MT+EQIFS+TQHLQQLLERFLACRPTGA
Sbjct: 181 NNNNNDNKNDDDNNDEDDNSKAIVVRATPIRDMTSEQIFSRTQHLQQLLERFLACRPTGA 240

Query: 241 AKNNRVVLVALYPVVKESFQIYYDITEIMGILIDRFMELNIPDCVKVYEIFCRVGKQFDE 300
           AKNNRVVLVALYP+V+ESFQIYYDITEIMGILIDRFMELN+ DCVKVYEIFCRVGKQFDE
Sbjct: 241 AKNNRVVLVALYPIVRESFQIYYDITEIMGILIDRFMELNVHDCVKVYEIFCRVGKQFDE 300

Query: 301 LDQLYSWCKTIGIARSSEYPEVEKITPKKLEVMDDFIKDKSALAQCNRDRPNKPLQLQIT 360
           L+  YSWC+TIGIARSSEYP+VEKITPKKLEVMD+FIKDKSALAQC   R    LQ++IT
Sbjct: 301 LELFYSWCRTIGIARSSEYPDVEKITPKKLEVMDEFIKDKSALAQCQTGRGKNQLQIEIT 360

Query: 361 EQEEEDSKQEQEPEDQDMNAIKALPPPEDFKPEQEPAAIEVKEE-SEPKEDEKKKDENPM 420
           E+ +++       E+ DMN+IKALPPPEDFK E++  AIEVKEE +E KED  KKDENP+
Sbjct: 361 EEVKKE-------EEIDMNSIKALPPPEDFK-EEDITAIEVKEEENEIKEDVIKKDENPL 420

Query: 421 MCDLLNLGEDHAIMREENADKLALALFDGSGPKVEALPAPT--WQAFNEETSDWETALVQ 480
           + DLLNLGE+HA++REENADKLALALFDG GP  ++    T  WQAFN+++ DWET LVQ
Sbjct: 421 IGDLLNLGEEHAMIREENADKLALALFDGIGPVTDSSSVKTLPWQAFNDDSQDWETTLVQ 480

Query: 481 SASNLSHQRAELGGGFDMLLLDGMYKQPTVVT-----GVGSSGSASSMALGSAGRPAMLA 540
           SASNLS+QRA+LGGGFDMLLLDGMYKQ T+++     G G SGSASSMALGSAGRPAMLA
Sbjct: 481 SASNLSNQRADLGGGFDMLLLDGMYKQTTMMSTMAGSGYGVSGSASSMALGSAGRPAMLA 540

Query: 541 LPAPPPPGEGGASSSSS---DPFAASVAVAPPSYVQMSEMERKQKLLVEEQLMWQQYARD 596
           LPAPP   EGG  SSSS   DPFAAS+AVAPP YVQMSEMERKQKLLVEEQLMWQQYARD
Sbjct: 541 LPAPPTITEGGGVSSSSVVSDPFAASMAVAPPPYVQMSEMERKQKLLVEEQLMWQQYARD 600

BLAST of MC00g0056 vs. NCBI nr
Match: XP_038888903.1 (putative clathrin assembly protein At1g03050 isoform X2 [Benincasa hispida])

HSP 1 Score: 909 bits (2348), Expect = 0.0
Identity = 489/607 (80.56%), Postives = 538/607 (88.63%), Query Frame = 0

Query: 1   MAPSKIRRALGAVKDKTSIGLAKVGSSTSLSDLEVAIVKATRHEEYPAEERHIREILSLT 60
           MAPSKIRRALGAVKDKTSIGLAKVGSSTSLSDLEVAIVKATRHEEYPAEERH+REILSLT
Sbjct: 1   MAPSKIRRALGAVKDKTSIGLAKVGSSTSLSDLEVAIVKATRHEEYPAEERHVREILSLT 60

Query: 61  CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVLEGDPAYEQEIFFSTRRGTRFL 120
           CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLV EGDPAYEQEIFFSTRRGTRFL
Sbjct: 61  CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVSEGDPAYEQEIFFSTRRGTRFL 120

Query: 121 NMSDFRDVSQSDSWDYSAFVRTYALYLDERLEYRMQSRRGKRSAFGIEDEEEQNNQNNIN 180
           NMSDFRD SQS+SWDYSAFVRT+ALYLDERLE+RMQSRRGKRSAFG++DEE Q  QNN  
Sbjct: 121 NMSDFRDTSQSNSWDYSAFVRTFALYLDERLEFRMQSRRGKRSAFGLDDEEGQQQQNN-- 180

Query: 181 NIDTNNNNNSQAIVVRAAPVQEMTTEQIFSKTQHLQQLLERFLACRPTGAAKNNRVVLVA 240
               + ++NS+AIVVRA P+++MT+EQIFS+TQHLQQLLERFLACRPTGAAKNNRVVLVA
Sbjct: 181 ----DEDDNSKAIVVRATPIRDMTSEQIFSRTQHLQQLLERFLACRPTGAAKNNRVVLVA 240

Query: 241 LYPVVKESFQIYYDITEIMGILIDRFMELNIPDCVKVYEIFCRVGKQFDELDQLYSWCKT 300
           LYP+V+ESFQIYYDITEIMGILIDRFMELN+ DCVKVYEIFCRVGKQFDEL+  YSWC+T
Sbjct: 241 LYPIVRESFQIYYDITEIMGILIDRFMELNVHDCVKVYEIFCRVGKQFDELELFYSWCRT 300

Query: 301 IGIARSSEYPEVEKITPKKLEVMDDFIKDKSALAQCNRDRPNKPLQLQITEQEEEDSKQE 360
           IGIARSSEYP+VEKITPKKLEVMD+FIKDKSALAQC   R    LQ++ITE+ +++    
Sbjct: 301 IGIARSSEYPDVEKITPKKLEVMDEFIKDKSALAQCQTGRGKNQLQIEITEEVKKE---- 360

Query: 361 QEPEDQDMNAIKALPPPEDFKPEQEPAAIEVKEE-SEPKEDEKKKDENPMMCDLLNLGED 420
              E+ DMN+IKALPPPEDFK E++  AIEVKEE +E KED  KKDENP++ DLLNLGE+
Sbjct: 361 ---EEIDMNSIKALPPPEDFK-EEDITAIEVKEEENEIKEDVIKKDENPLIGDLLNLGEE 420

Query: 421 HAIMREENADKLALALFDGSGPKVEALPAPT--WQAFNEETSDWETALVQSASNLSHQRA 480
           HA++REENADKLALALFDG GP  ++    T  WQAFN+++ DWET LVQSASNLS+QRA
Sbjct: 421 HAMIREENADKLALALFDGIGPVTDSSSVKTLPWQAFNDDSQDWETTLVQSASNLSNQRA 480

Query: 481 ELGGGFDMLLLDGMYKQPTVVT-----GVGSSGSASSMALGSAGRPAMLALPAPPPPGEG 540
           +LGGGFDMLLLDGMYKQ T+++     G G SGSASSMALGSAGRPAMLALPAPP   EG
Sbjct: 481 DLGGGFDMLLLDGMYKQTTMMSTMAGSGYGVSGSASSMALGSAGRPAMLALPAPPTITEG 540

Query: 541 GASSSSS---DPFAASVAVAPPSYVQMSEMERKQKLLVEEQLMWQQYARDGRQIQHNPYA 596
           G  SSSS   DPFAAS+AVAPP YVQMSEMERKQKLLVEEQLMWQQYARDGRQIQHNPY 
Sbjct: 541 GGVSSSSVVSDPFAASMAVAPPPYVQMSEMERKQKLLVEEQLMWQQYARDGRQIQHNPYT 593

BLAST of MC00g0056 vs. NCBI nr
Match: XP_008455244.1 (PREDICTED: putative clathrin assembly protein At1g03050 [Cucumis melo] >KAA0031514.1 putative clathrin assembly protein [Cucumis melo var. makuwa] >TYK06967.1 putative clathrin assembly protein [Cucumis melo var. makuwa])

HSP 1 Score: 909 bits (2348), Expect = 0.0
Identity = 493/615 (80.16%), Postives = 540/615 (87.80%), Query Frame = 0

Query: 1   MAPSKIRRALGAVKDKTSIGLAKVGSSTSLSDLEVAIVKATRHEEYPAEERHIREILSLT 60
           MAPSKIRRALGAVKDKTSIGLAKVGSS SLSDLEVAIVKATRHEEYPAEERH+REILSLT
Sbjct: 1   MAPSKIRRALGAVKDKTSIGLAKVGSSNSLSDLEVAIVKATRHEEYPAEERHVREILSLT 60

Query: 61  CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVLEGDPAYEQEIFFSTRRGTRFL 120
           CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLV EGDPAYEQEIFFSTRRGTRFL
Sbjct: 61  CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVSEGDPAYEQEIFFSTRRGTRFL 120

Query: 121 NMSDFRDVSQSDSWDYSAFVRTYALYLDERLEYRMQSRRGKRSAFGIEDEEEQNNQ---- 180
           NMSDFRD S S+SWDYSAFVRTYALYLDERLE+RMQSRRG+RSAFG+++E+E ++     
Sbjct: 121 NMSDFRDASLSNSWDYSAFVRTYALYLDERLEFRMQSRRGRRSAFGLDEEDEHHHHQQQN 180

Query: 181 ---NNINNIDTNNNN---NSQAIVVRAAPVQEMTTEQIFSKTQHLQQLLERFLACRPTGA 240
              +N NNID +NN+   NS+AIV+RA P+++MT++QIFS+TQHLQQLLERFLACRPTGA
Sbjct: 181 SCSSNNNNIDNDNNDEEDNSKAIVIRATPIRDMTSDQIFSRTQHLQQLLERFLACRPTGA 240

Query: 241 AKNNRVVLVALYPVVKESFQIYYDITEIMGILIDRFMELNIPDCVKVYEIFCRVGKQFDE 300
           AKNNRVVLVALYP+V+ESFQIYYDITEIMGILIDRFMEL I DCVKVYEIFCRVGKQFDE
Sbjct: 241 AKNNRVVLVALYPIVRESFQIYYDITEIMGILIDRFMELKIQDCVKVYEIFCRVGKQFDE 300

Query: 301 LDQLYSWCKTIGIARSSEYPEVEKITPKKLEVMDDFIKDKSALAQCNRDRPNKPLQLQIT 360
           L+  YSWCKTIGIARSSEYPEVEKITPKKLEVMD+FIKD+SALAQC +DR     QL+I 
Sbjct: 301 LELFYSWCKTIGIARSSEYPEVEKITPKKLEVMDEFIKDRSALAQCQKDREKAQPQLEIA 360

Query: 361 EQEEEDSKQEQEPEDQDMNAIKALPPPEDFKPEQEPAAIEVKEES-EPKEDEKKKD-ENP 420
           E+E +      E E++DMN++KALPPPED K E+E  A EVKEE  E KEDE KKD EN 
Sbjct: 361 EEEHK-----VEEEEEDMNSVKALPPPEDVK-EEETTATEVKEEEKEIKEDEMKKDNENS 420

Query: 421 MMCDLLNLGEDHAIMREENADKLALALFDGSGPKVEALPAPTWQAFNEETSDWETALVQS 480
           MM DLLNLGE+HA++REENADKLALALFDGSGP   ++ A  WQAFN+E++DWE+ALVQS
Sbjct: 421 MMGDLLNLGEEHAMIREENADKLALALFDGSGPVDSSVKALPWQAFNDESADWESALVQS 480

Query: 481 ASNLSHQRAELGGGFDMLLLDGMYKQPTVVT-----GVGSSGSASSMALGSAGRPAMLAL 540
           ASNLS+QRA+LGGGFDMLLLDGMYKQ  V +     G G SGSASSMALGSAGRPAMLAL
Sbjct: 481 ASNLSNQRADLGGGFDMLLLDGMYKQTAVKSTMAGSGYGVSGSASSMALGSAGRPAMLAL 540

Query: 541 PAPPPPGEGGASSSS--SDPFAASVAVAPPSYVQMSEMERKQKLLVEEQLMWQQYARDGR 596
           PAPP P   G SSSS  SDPFAASVAVAPP YVQMSEMERKQKLLVEEQLMWQQYARDGR
Sbjct: 541 PAPPTPEGSGTSSSSVVSDPFAASVAVAPPPYVQMSEMERKQKLLVEEQLMWQQYARDGR 600

BLAST of MC00g0056 vs. NCBI nr
Match: XP_011658768.1 (putative clathrin assembly protein At1g03050 [Cucumis sativus] >KGN43681.1 hypothetical protein Csa_017299 [Cucumis sativus])

HSP 1 Score: 899 bits (2323), Expect = 0.0
Identity = 489/622 (78.62%), Postives = 541/622 (86.98%), Query Frame = 0

Query: 1   MAPSKIRRALGAVKDKTSIGLAKVGSSTSLSDLEVAIVKATRHEEYPAEERHIREILSLT 60
           MAPSKIRRALGAVKDKTSIGLAKVGSSTSLSDLEVAIVKATRHEEYPAEERH+REILSLT
Sbjct: 1   MAPSKIRRALGAVKDKTSIGLAKVGSSTSLSDLEVAIVKATRHEEYPAEERHVREILSLT 60

Query: 61  CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVLEGDPAYEQEIFFSTRRGTRFL 120
           CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLV EGDPAYEQEIFFSTRRGTRFL
Sbjct: 61  CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVSEGDPAYEQEIFFSTRRGTRFL 120

Query: 121 NMSDFRDVSQSDSWDYSAFVRTYALYLDERLEYRMQSRRGKRSAFGIEDEEEQ------- 180
           NMSDFRD SQS+SWDYSAFVRTYALYLDERLE+RMQSRRG+RSAFG+++E+E        
Sbjct: 121 NMSDFRDTSQSNSWDYSAFVRTYALYLDERLEFRMQSRRGRRSAFGLDEEDEHHHHHQQQ 180

Query: 181 ----NNQNNINNIDTNNNNN------SQAIVVRAAPVQEMTTEQIFSKTQHLQQLLERFL 240
               N+ ++ NN D N+N+N      S+AI+VRA P+++MT+EQIFS+TQHLQQLLERFL
Sbjct: 181 QQHINSSSSNNNEDNNDNDNNDEEDHSKAIIVRATPMRDMTSEQIFSRTQHLQQLLERFL 240

Query: 241 ACRPTGAAKNNRVVLVALYPVVKESFQIYYDITEIMGILIDRFMELNIPDCVKVYEIFCR 300
           ACRPTGAAKNNRVVLVALYPVV+ESFQIYYDITEIMGILIDRFM+LN+ DCVKVYEIFCR
Sbjct: 241 ACRPTGAAKNNRVVLVALYPVVRESFQIYYDITEIMGILIDRFMDLNVQDCVKVYEIFCR 300

Query: 301 VGKQFDELDQLYSWCKTIGIARSSEYPEVEKITPKKLEVMDDFIKDKSALAQCNRDRPNK 360
           VGKQFDEL+  YSWC+TIGIARS+EYPEVEKITPKKLEVMD+FIKD+SALAQC  DR   
Sbjct: 301 VGKQFDELEMFYSWCRTIGIARSAEYPEVEKITPKKLEVMDEFIKDRSALAQCQNDREKT 360

Query: 361 PLQLQITEQEEEDSKQEQEPEDQDMNAIKALPPPEDFKPEQEPAAIEVKEES-EPKEDEK 420
             QL+ITE+E    K+E+E E+ D+N+IKALPPPED K E+E  AIEV+EE  E KEDE 
Sbjct: 361 QPQLEITEEEH---KEEEEEEEVDVNSIKALPPPEDIK-EEEITAIEVEEEEKEIKEDEM 420

Query: 421 KKD-ENPMMCDLLNLGEDHAIMREENADKLALALFDGSGPKVEALPAPTWQAFNEETSDW 480
           KKD +N MM DLLNLGE+HA++RE NADKLA ALFDGSGP   ++ A  WQAFN++++DW
Sbjct: 421 KKDNDNSMMGDLLNLGEEHAMIREANADKLATALFDGSGPVDSSVKALPWQAFNDDSADW 480

Query: 481 ETALVQSASNLSHQRAELGGGFDMLLLDGMYKQPTVVT-----GVGSSGSASSMALGSAG 540
           ETALVQSAS+L +Q+A+LGGGFDMLLLDGMYKQ  V +     G G SGSASSMALGSAG
Sbjct: 481 ETALVQSASHLPNQKADLGGGFDMLLLDGMYKQTAVKSTMAGSGYGVSGSASSMALGSAG 540

Query: 541 RPAMLALPAPPPPGEGGASSSS--SDPFAASVAVAPPSYVQMSEMERKQKLLVEEQLMWQ 596
           RPAMLALPAP  P   G SSSS  SDPFAASVAVAPP YVQMSEMERKQKLLVEEQLMWQ
Sbjct: 541 RPAMLALPAPTTPEGSGTSSSSVVSDPFAASVAVAPPPYVQMSEMERKQKLLVEEQLMWQ 600

BLAST of MC00g0056 vs. ExPASy TrEMBL
Match: A0A6J1DLL6 (putative clathrin assembly protein At1g03050 OS=Momordica charantia OX=3673 GN=LOC111021688 PE=4 SV=1)

HSP 1 Score: 1147 bits (2966), Expect = 0.0
Identity = 596/596 (100.00%), Postives = 596/596 (100.00%), Query Frame = 0

Query: 1   MAPSKIRRALGAVKDKTSIGLAKVGSSTSLSDLEVAIVKATRHEEYPAEERHIREILSLT 60
           MAPSKIRRALGAVKDKTSIGLAKVGSSTSLSDLEVAIVKATRHEEYPAEERHIREILSLT
Sbjct: 1   MAPSKIRRALGAVKDKTSIGLAKVGSSTSLSDLEVAIVKATRHEEYPAEERHIREILSLT 60

Query: 61  CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVLEGDPAYEQEIFFSTRRGTRFL 120
           CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVLEGDPAYEQEIFFSTRRGTRFL
Sbjct: 61  CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVLEGDPAYEQEIFFSTRRGTRFL 120

Query: 121 NMSDFRDVSQSDSWDYSAFVRTYALYLDERLEYRMQSRRGKRSAFGIEDEEEQNNQNNIN 180
           NMSDFRDVSQSDSWDYSAFVRTYALYLDERLEYRMQSRRGKRSAFGIEDEEEQNNQNNIN
Sbjct: 121 NMSDFRDVSQSDSWDYSAFVRTYALYLDERLEYRMQSRRGKRSAFGIEDEEEQNNQNNIN 180

Query: 181 NIDTNNNNNSQAIVVRAAPVQEMTTEQIFSKTQHLQQLLERFLACRPTGAAKNNRVVLVA 240
           NIDTNNNNNSQAIVVRAAPVQEMTTEQIFSKTQHLQQLLERFLACRPTGAAKNNRVVLVA
Sbjct: 181 NIDTNNNNNSQAIVVRAAPVQEMTTEQIFSKTQHLQQLLERFLACRPTGAAKNNRVVLVA 240

Query: 241 LYPVVKESFQIYYDITEIMGILIDRFMELNIPDCVKVYEIFCRVGKQFDELDQLYSWCKT 300
           LYPVVKESFQIYYDITEIMGILIDRFMELNIPDCVKVYEIFCRVGKQFDELDQLYSWCKT
Sbjct: 241 LYPVVKESFQIYYDITEIMGILIDRFMELNIPDCVKVYEIFCRVGKQFDELDQLYSWCKT 300

Query: 301 IGIARSSEYPEVEKITPKKLEVMDDFIKDKSALAQCNRDRPNKPLQLQITEQEEEDSKQE 360
           IGIARSSEYPEVEKITPKKLEVMDDFIKDKSALAQCNRDRPNKPLQLQITEQEEEDSKQE
Sbjct: 301 IGIARSSEYPEVEKITPKKLEVMDDFIKDKSALAQCNRDRPNKPLQLQITEQEEEDSKQE 360

Query: 361 QEPEDQDMNAIKALPPPEDFKPEQEPAAIEVKEESEPKEDEKKKDENPMMCDLLNLGEDH 420
           QEPEDQDMNAIKALPPPEDFKPEQEPAAIEVKEESEPKEDEKKKDENPMMCDLLNLGEDH
Sbjct: 361 QEPEDQDMNAIKALPPPEDFKPEQEPAAIEVKEESEPKEDEKKKDENPMMCDLLNLGEDH 420

Query: 421 AIMREENADKLALALFDGSGPKVEALPAPTWQAFNEETSDWETALVQSASNLSHQRAELG 480
           AIMREENADKLALALFDGSGPKVEALPAPTWQAFNEETSDWETALVQSASNLSHQRAELG
Sbjct: 421 AIMREENADKLALALFDGSGPKVEALPAPTWQAFNEETSDWETALVQSASNLSHQRAELG 480

Query: 481 GGFDMLLLDGMYKQPTVVTGVGSSGSASSMALGSAGRPAMLALPAPPPPGEGGASSSSSD 540
           GGFDMLLLDGMYKQPTVVTGVGSSGSASSMALGSAGRPAMLALPAPPPPGEGGASSSSSD
Sbjct: 481 GGFDMLLLDGMYKQPTVVTGVGSSGSASSMALGSAGRPAMLALPAPPPPGEGGASSSSSD 540

Query: 541 PFAASVAVAPPSYVQMSEMERKQKLLVEEQLMWQQYARDGRQIQHNPYAGGFTHSY 596
           PFAASVAVAPPSYVQMSEMERKQKLLVEEQLMWQQYARDGRQIQHNPYAGGFTHSY
Sbjct: 541 PFAASVAVAPPSYVQMSEMERKQKLLVEEQLMWQQYARDGRQIQHNPYAGGFTHSY 596

BLAST of MC00g0056 vs. ExPASy TrEMBL
Match: A0A5A7SK49 (Putative clathrin assembly protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G002810 PE=4 SV=1)

HSP 1 Score: 909 bits (2348), Expect = 0.0
Identity = 493/615 (80.16%), Postives = 540/615 (87.80%), Query Frame = 0

Query: 1   MAPSKIRRALGAVKDKTSIGLAKVGSSTSLSDLEVAIVKATRHEEYPAEERHIREILSLT 60
           MAPSKIRRALGAVKDKTSIGLAKVGSS SLSDLEVAIVKATRHEEYPAEERH+REILSLT
Sbjct: 1   MAPSKIRRALGAVKDKTSIGLAKVGSSNSLSDLEVAIVKATRHEEYPAEERHVREILSLT 60

Query: 61  CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVLEGDPAYEQEIFFSTRRGTRFL 120
           CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLV EGDPAYEQEIFFSTRRGTRFL
Sbjct: 61  CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVSEGDPAYEQEIFFSTRRGTRFL 120

Query: 121 NMSDFRDVSQSDSWDYSAFVRTYALYLDERLEYRMQSRRGKRSAFGIEDEEEQNNQ---- 180
           NMSDFRD S S+SWDYSAFVRTYALYLDERLE+RMQSRRG+RSAFG+++E+E ++     
Sbjct: 121 NMSDFRDASLSNSWDYSAFVRTYALYLDERLEFRMQSRRGRRSAFGLDEEDEHHHHQQQN 180

Query: 181 ---NNINNIDTNNNN---NSQAIVVRAAPVQEMTTEQIFSKTQHLQQLLERFLACRPTGA 240
              +N NNID +NN+   NS+AIV+RA P+++MT++QIFS+TQHLQQLLERFLACRPTGA
Sbjct: 181 SCSSNNNNIDNDNNDEEDNSKAIVIRATPIRDMTSDQIFSRTQHLQQLLERFLACRPTGA 240

Query: 241 AKNNRVVLVALYPVVKESFQIYYDITEIMGILIDRFMELNIPDCVKVYEIFCRVGKQFDE 300
           AKNNRVVLVALYP+V+ESFQIYYDITEIMGILIDRFMEL I DCVKVYEIFCRVGKQFDE
Sbjct: 241 AKNNRVVLVALYPIVRESFQIYYDITEIMGILIDRFMELKIQDCVKVYEIFCRVGKQFDE 300

Query: 301 LDQLYSWCKTIGIARSSEYPEVEKITPKKLEVMDDFIKDKSALAQCNRDRPNKPLQLQIT 360
           L+  YSWCKTIGIARSSEYPEVEKITPKKLEVMD+FIKD+SALAQC +DR     QL+I 
Sbjct: 301 LELFYSWCKTIGIARSSEYPEVEKITPKKLEVMDEFIKDRSALAQCQKDREKAQPQLEIA 360

Query: 361 EQEEEDSKQEQEPEDQDMNAIKALPPPEDFKPEQEPAAIEVKEES-EPKEDEKKKD-ENP 420
           E+E +      E E++DMN++KALPPPED K E+E  A EVKEE  E KEDE KKD EN 
Sbjct: 361 EEEHK-----VEEEEEDMNSVKALPPPEDVK-EEETTATEVKEEEKEIKEDEMKKDNENS 420

Query: 421 MMCDLLNLGEDHAIMREENADKLALALFDGSGPKVEALPAPTWQAFNEETSDWETALVQS 480
           MM DLLNLGE+HA++REENADKLALALFDGSGP   ++ A  WQAFN+E++DWE+ALVQS
Sbjct: 421 MMGDLLNLGEEHAMIREENADKLALALFDGSGPVDSSVKALPWQAFNDESADWESALVQS 480

Query: 481 ASNLSHQRAELGGGFDMLLLDGMYKQPTVVT-----GVGSSGSASSMALGSAGRPAMLAL 540
           ASNLS+QRA+LGGGFDMLLLDGMYKQ  V +     G G SGSASSMALGSAGRPAMLAL
Sbjct: 481 ASNLSNQRADLGGGFDMLLLDGMYKQTAVKSTMAGSGYGVSGSASSMALGSAGRPAMLAL 540

Query: 541 PAPPPPGEGGASSSS--SDPFAASVAVAPPSYVQMSEMERKQKLLVEEQLMWQQYARDGR 596
           PAPP P   G SSSS  SDPFAASVAVAPP YVQMSEMERKQKLLVEEQLMWQQYARDGR
Sbjct: 541 PAPPTPEGSGTSSSSVVSDPFAASVAVAPPPYVQMSEMERKQKLLVEEQLMWQQYARDGR 600

BLAST of MC00g0056 vs. ExPASy TrEMBL
Match: A0A1S3C1P9 (putative clathrin assembly protein At1g03050 OS=Cucumis melo OX=3656 GN=LOC103495455 PE=4 SV=1)

HSP 1 Score: 909 bits (2348), Expect = 0.0
Identity = 493/615 (80.16%), Postives = 540/615 (87.80%), Query Frame = 0

Query: 1   MAPSKIRRALGAVKDKTSIGLAKVGSSTSLSDLEVAIVKATRHEEYPAEERHIREILSLT 60
           MAPSKIRRALGAVKDKTSIGLAKVGSS SLSDLEVAIVKATRHEEYPAEERH+REILSLT
Sbjct: 1   MAPSKIRRALGAVKDKTSIGLAKVGSSNSLSDLEVAIVKATRHEEYPAEERHVREILSLT 60

Query: 61  CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVLEGDPAYEQEIFFSTRRGTRFL 120
           CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLV EGDPAYEQEIFFSTRRGTRFL
Sbjct: 61  CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVSEGDPAYEQEIFFSTRRGTRFL 120

Query: 121 NMSDFRDVSQSDSWDYSAFVRTYALYLDERLEYRMQSRRGKRSAFGIEDEEEQNNQ---- 180
           NMSDFRD S S+SWDYSAFVRTYALYLDERLE+RMQSRRG+RSAFG+++E+E ++     
Sbjct: 121 NMSDFRDASLSNSWDYSAFVRTYALYLDERLEFRMQSRRGRRSAFGLDEEDEHHHHQQQN 180

Query: 181 ---NNINNIDTNNNN---NSQAIVVRAAPVQEMTTEQIFSKTQHLQQLLERFLACRPTGA 240
              +N NNID +NN+   NS+AIV+RA P+++MT++QIFS+TQHLQQLLERFLACRPTGA
Sbjct: 181 SCSSNNNNIDNDNNDEEDNSKAIVIRATPIRDMTSDQIFSRTQHLQQLLERFLACRPTGA 240

Query: 241 AKNNRVVLVALYPVVKESFQIYYDITEIMGILIDRFMELNIPDCVKVYEIFCRVGKQFDE 300
           AKNNRVVLVALYP+V+ESFQIYYDITEIMGILIDRFMEL I DCVKVYEIFCRVGKQFDE
Sbjct: 241 AKNNRVVLVALYPIVRESFQIYYDITEIMGILIDRFMELKIQDCVKVYEIFCRVGKQFDE 300

Query: 301 LDQLYSWCKTIGIARSSEYPEVEKITPKKLEVMDDFIKDKSALAQCNRDRPNKPLQLQIT 360
           L+  YSWCKTIGIARSSEYPEVEKITPKKLEVMD+FIKD+SALAQC +DR     QL+I 
Sbjct: 301 LELFYSWCKTIGIARSSEYPEVEKITPKKLEVMDEFIKDRSALAQCQKDREKAQPQLEIA 360

Query: 361 EQEEEDSKQEQEPEDQDMNAIKALPPPEDFKPEQEPAAIEVKEES-EPKEDEKKKD-ENP 420
           E+E +      E E++DMN++KALPPPED K E+E  A EVKEE  E KEDE KKD EN 
Sbjct: 361 EEEHK-----VEEEEEDMNSVKALPPPEDVK-EEETTATEVKEEEKEIKEDEMKKDNENS 420

Query: 421 MMCDLLNLGEDHAIMREENADKLALALFDGSGPKVEALPAPTWQAFNEETSDWETALVQS 480
           MM DLLNLGE+HA++REENADKLALALFDGSGP   ++ A  WQAFN+E++DWE+ALVQS
Sbjct: 421 MMGDLLNLGEEHAMIREENADKLALALFDGSGPVDSSVKALPWQAFNDESADWESALVQS 480

Query: 481 ASNLSHQRAELGGGFDMLLLDGMYKQPTVVT-----GVGSSGSASSMALGSAGRPAMLAL 540
           ASNLS+QRA+LGGGFDMLLLDGMYKQ  V +     G G SGSASSMALGSAGRPAMLAL
Sbjct: 481 ASNLSNQRADLGGGFDMLLLDGMYKQTAVKSTMAGSGYGVSGSASSMALGSAGRPAMLAL 540

Query: 541 PAPPPPGEGGASSSS--SDPFAASVAVAPPSYVQMSEMERKQKLLVEEQLMWQQYARDGR 596
           PAPP P   G SSSS  SDPFAASVAVAPP YVQMSEMERKQKLLVEEQLMWQQYARDGR
Sbjct: 541 PAPPTPEGSGTSSSSVVSDPFAASVAVAPPPYVQMSEMERKQKLLVEEQLMWQQYARDGR 600

BLAST of MC00g0056 vs. ExPASy TrEMBL
Match: A0A0A0K783 (ENTH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G058550 PE=4 SV=1)

HSP 1 Score: 899 bits (2323), Expect = 0.0
Identity = 489/622 (78.62%), Postives = 541/622 (86.98%), Query Frame = 0

Query: 1   MAPSKIRRALGAVKDKTSIGLAKVGSSTSLSDLEVAIVKATRHEEYPAEERHIREILSLT 60
           MAPSKIRRALGAVKDKTSIGLAKVGSSTSLSDLEVAIVKATRHEEYPAEERH+REILSLT
Sbjct: 1   MAPSKIRRALGAVKDKTSIGLAKVGSSTSLSDLEVAIVKATRHEEYPAEERHVREILSLT 60

Query: 61  CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVLEGDPAYEQEIFFSTRRGTRFL 120
           CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLV EGDPAYEQEIFFSTRRGTRFL
Sbjct: 61  CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVSEGDPAYEQEIFFSTRRGTRFL 120

Query: 121 NMSDFRDVSQSDSWDYSAFVRTYALYLDERLEYRMQSRRGKRSAFGIEDEEEQ------- 180
           NMSDFRD SQS+SWDYSAFVRTYALYLDERLE+RMQSRRG+RSAFG+++E+E        
Sbjct: 121 NMSDFRDTSQSNSWDYSAFVRTYALYLDERLEFRMQSRRGRRSAFGLDEEDEHHHHHQQQ 180

Query: 181 ----NNQNNINNIDTNNNNN------SQAIVVRAAPVQEMTTEQIFSKTQHLQQLLERFL 240
               N+ ++ NN D N+N+N      S+AI+VRA P+++MT+EQIFS+TQHLQQLLERFL
Sbjct: 181 QQHINSSSSNNNEDNNDNDNNDEEDHSKAIIVRATPMRDMTSEQIFSRTQHLQQLLERFL 240

Query: 241 ACRPTGAAKNNRVVLVALYPVVKESFQIYYDITEIMGILIDRFMELNIPDCVKVYEIFCR 300
           ACRPTGAAKNNRVVLVALYPVV+ESFQIYYDITEIMGILIDRFM+LN+ DCVKVYEIFCR
Sbjct: 241 ACRPTGAAKNNRVVLVALYPVVRESFQIYYDITEIMGILIDRFMDLNVQDCVKVYEIFCR 300

Query: 301 VGKQFDELDQLYSWCKTIGIARSSEYPEVEKITPKKLEVMDDFIKDKSALAQCNRDRPNK 360
           VGKQFDEL+  YSWC+TIGIARS+EYPEVEKITPKKLEVMD+FIKD+SALAQC  DR   
Sbjct: 301 VGKQFDELEMFYSWCRTIGIARSAEYPEVEKITPKKLEVMDEFIKDRSALAQCQNDREKT 360

Query: 361 PLQLQITEQEEEDSKQEQEPEDQDMNAIKALPPPEDFKPEQEPAAIEVKEES-EPKEDEK 420
             QL+ITE+E    K+E+E E+ D+N+IKALPPPED K E+E  AIEV+EE  E KEDE 
Sbjct: 361 QPQLEITEEEH---KEEEEEEEVDVNSIKALPPPEDIK-EEEITAIEVEEEEKEIKEDEM 420

Query: 421 KKD-ENPMMCDLLNLGEDHAIMREENADKLALALFDGSGPKVEALPAPTWQAFNEETSDW 480
           KKD +N MM DLLNLGE+HA++RE NADKLA ALFDGSGP   ++ A  WQAFN++++DW
Sbjct: 421 KKDNDNSMMGDLLNLGEEHAMIREANADKLATALFDGSGPVDSSVKALPWQAFNDDSADW 480

Query: 481 ETALVQSASNLSHQRAELGGGFDMLLLDGMYKQPTVVT-----GVGSSGSASSMALGSAG 540
           ETALVQSAS+L +Q+A+LGGGFDMLLLDGMYKQ  V +     G G SGSASSMALGSAG
Sbjct: 481 ETALVQSASHLPNQKADLGGGFDMLLLDGMYKQTAVKSTMAGSGYGVSGSASSMALGSAG 540

Query: 541 RPAMLALPAPPPPGEGGASSSS--SDPFAASVAVAPPSYVQMSEMERKQKLLVEEQLMWQ 596
           RPAMLALPAP  P   G SSSS  SDPFAASVAVAPP YVQMSEMERKQKLLVEEQLMWQ
Sbjct: 541 RPAMLALPAPTTPEGSGTSSSSVVSDPFAASVAVAPPPYVQMSEMERKQKLLVEEQLMWQ 600

BLAST of MC00g0056 vs. ExPASy TrEMBL
Match: A0A6J1HJ28 (putative clathrin assembly protein At1g03050 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111464026 PE=4 SV=1)

HSP 1 Score: 865 bits (2234), Expect = 3.37e-311
Identity = 471/621 (75.85%), Postives = 530/621 (85.35%), Query Frame = 0

Query: 1   MAPSKIRRALGAVKDKTSIGLAKVGSSTSLSDLEVAIVKATRHEEYPAEERHIREILSLT 60
           MAPSKIRRALGAVKDKTSIGLAKVGSS SLSDLEVAIVKATRHEEYPAEERH+REILSLT
Sbjct: 1   MAPSKIRRALGAVKDKTSIGLAKVGSSNSLSDLEVAIVKATRHEEYPAEERHVREILSLT 60

Query: 61  CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVLEGDPAYEQEIFFSTRRGTRFL 120
           CYS+AYISACVNTLSRRLNKTK+WTVALKTLMLIQRLV EGDPAYEQEIFFSTRRGTRFL
Sbjct: 61  CYSKAYISACVNTLSRRLNKTKDWTVALKTLMLIQRLVSEGDPAYEQEIFFSTRRGTRFL 120

Query: 121 NMSDFRDVSQSDSWDYSAFVRTYALYLDERLEYRMQSRRGKRSAFGIEDEEEQ------- 180
           NMSDFRD SQS+SWDYSAFVRTYALYLDERLEYRMQSRRG+RSAFG+ +EE+Q       
Sbjct: 121 NMSDFRDTSQSNSWDYSAFVRTYALYLDERLEYRMQSRRGRRSAFGLLEEEDQQQNSNRN 180

Query: 181 NNQNNINNIDTNNNNNSQAIVVRAAPVQEMTTEQIFSKTQHLQQLLERFLACRPTGAAKN 240
           +N +N NN D ++N+N++ I++RA P+ EMTTEQIFSKTQHLQQLLERFLACRPTGAAK 
Sbjct: 181 SNSSNTNNDDDDDNDNNKTIMIRATPLHEMTTEQIFSKTQHLQQLLERFLACRPTGAAKV 240

Query: 241 NRVVLVALYPVVKESFQIYYDITEIMGILIDRFMELNIPDCVKVYEIFCRVGKQFDELDQ 300
           NRVVLVALYP+VKESFQIYYDITE MGILIDRFM+L I DCV+VYEIFCRVGKQFDEL+ 
Sbjct: 241 NRVVLVALYPIVKESFQIYYDITETMGILIDRFMDLKIRDCVRVYEIFCRVGKQFDELEM 300

Query: 301 LYSWCKTIGIARSSEYPEVEKITPKKLEVMDDFIKDKSALAQCNRDRPNKPLQLQITEQE 360
            YSWC+ IGIARS+EYPEVEKITPKKLEVMD+FIKDKSALAQC R+      +++ITE+E
Sbjct: 301 FYSWCRNIGIARSTEYPEVEKITPKKLEVMDEFIKDKSALAQCLRN------EVEITEEE 360

Query: 361 EEDSKQEQEPEDQ------DMNAIKALPPPEDFKPEQEPAAIEVKEESEPKE---DEKKK 420
           EE+ ++E+E ED+      DMN+IKALPPPE F+ EQ   A+ V EE+E  E    EK  
Sbjct: 361 EEEEEEEEEDEDEEEEQEHDMNSIKALPPPESFEEEQ--TALLVNEENETNEMKAKEKSD 420

Query: 421 DENPMMCDLLNLGEDHAIMREENADKLALALFDGSGPKVEA-LPAPTWQAFNEETSDWET 480
           +  PMM D LNL E+H + REENA+KLA ALFDGS P ++A +PA  W+AF+++  DWET
Sbjct: 421 EHYPMMGDFLNLDEEHQMTREENAEKLAFALFDGSAPIIDASVPALPWEAFSDDAPDWET 480

Query: 481 ALVQSASNLSHQRAELGGGFDMLLLDGMYKQPTVVT-----GVGSSGSASSMALGSAGRP 540
           ALVQSASNLS+QR +LGGGFDMLLLDGMYKQ T ++     G G SGSASSMALGSAGRP
Sbjct: 481 ALVQSASNLSNQRTDLGGGFDMLLLDGMYKQTTTMSTMAGSGYGVSGSASSMALGSAGRP 540

Query: 541 AMLALPAPPPPGEGGASSSSS---DPFAASVAVAPPSYVQMSEMERKQKLLVEEQLMWQQ 596
           A+LALPAP    EG  +SSSS   DPFAASVAVAPP+YVQMSEMERK+KLLVEEQLMWQQ
Sbjct: 541 ALLALPAPST-SEGSVASSSSVMSDPFAASVAVAPPAYVQMSEMERKKKLLVEEQLMWQQ 600

BLAST of MC00g0056 vs. TAIR 10
Match: AT1G03050.1 (ENTH/ANTH/VHS superfamily protein )

HSP 1 Score: 667.9 bits (1722), Expect = 7.4e-192
Identity = 386/604 (63.91%), Postives = 470/604 (77.81%), Query Frame = 0

Query: 1   MAPSKIRRALGAVKDKTSIGLAKV-GSSTSLSDLEVAIVKATRHEEYPAEERHIREILSL 60
           M  SK +RA+GAVKD+TS+GLAKV G S SLS+L+VAIVKATRHEE+PAEE++IREILSL
Sbjct: 1   MGSSKFKRAIGAVKDQTSVGLAKVNGRSASLSELDVAIVKATRHEEFPAEEKYIREILSL 60

Query: 61  TCYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVLEGDPAYEQEIFFSTRRGTRF 120
           T YSR+YI+ACV+TLSRRLNKTK WTVALKTL+LIQRL+ EGD AYEQEIFF+TRRGTR 
Sbjct: 61  TSYSRSYINACVSTLSRRLNKTKCWTVALKTLILIQRLLGEGDQAYEQEIFFATRRGTRL 120

Query: 121 LNMSDFRDVSQSDSWDYSAFVRTYALYLDERLEYRMQSRRGKRSAF---GIEDEEEQNNQ 180
           LNMSDFRDVS+S+SWDYSAFVRTYALYLDERL++RMQ+R GKR  +   G  DEEEQ   
Sbjct: 121 LNMSDFRDVSRSNSWDYSAFVRTYALYLDERLDFRMQARHGKRGVYCVGGEADEEEQ--- 180

Query: 181 NNINNIDTNNNNNSQAIVVRAAPVQEMTTEQIFSKTQHLQQLLERFLACRPTGAAKNNRV 240
                 D    + S AIVVR+ P+ EM TEQIF + QHLQQLL+RFLACRPTG A+NNRV
Sbjct: 181 ------DQAAADLSTAIVVRSQPIAEMKTEQIFIRIQHLQQLLDRFLACRPTGNARNNRV 240

Query: 241 VLVALYPVVKESFQIYYDITEIMGILIDRFMELNIPDCVKVYEIFCRVGKQFDELDQLYS 300
           V+VALYP+VKESFQIYYD+TEIMGILI+RFMEL+IPD +KVY+IFCRV KQF+ELDQ YS
Sbjct: 241 VIVALYPIVKESFQIYYDVTEIMGILIERFMELDIPDSIKVYDIFCRVSKQFEELDQFYS 300

Query: 301 WCKTIGIARSSEYPEVEKITPKKLEVMDDFIKDKSALAQCNRDRPNKPLQLQITEQEEED 360
           WCK +GIARSSEYPE+EKIT KKL++MD+FI+DKSAL      + +K ++ +  E ++E 
Sbjct: 301 WCKNMGIARSSEYPEIEKITQKKLDLMDEFIRDKSAL---EHTKQSKSVKSEADEDDDEA 360

Query: 361 SKQEQEPEDQDMNAIKALPPPEDFKPEQEPAAIEVKEESEPKED---EKKKDENPMMCDL 420
             +E   E +DMNAIKALP P    P++E    +VK E E KE+   EKK++E   M DL
Sbjct: 361 RTEEVNEEQEDMNAIKALPEP---PPKEED---DVKPEEEAKEEVIIEKKQEE---MGDL 420

Query: 421 LNLGEDHAIMREENADKLALALFDG---SGPKVEALPAPTWQAFNEETSDWETALVQSAS 480
           L+LG  +     +  D LALALFDG   SG   E+   P W+AF ++++DWETALVQ+A+
Sbjct: 421 LDLGNTNGGEAGQAGDSLALALFDGPYASGSGSES--GPGWEAFKDDSADWETALVQTAT 480

Query: 481 NLSHQRAELGGGFDMLLLDGMYKQPTV------VTGVGSSGSASSMALGSAGRPA--MLA 540
           NLS Q++ELGGGFDMLLL+GMY+   V       T  G+SGSASSMA GSAGRPA  MLA
Sbjct: 481 NLSGQKSELGGGFDMLLLNGMYQHGAVNAAVKTSTAYGASGSASSMAFGSAGRPAATMLA 540

Query: 541 LPAPPPPGEGGASSSSS----DPFAASVAVAPPSYVQMSEMERKQKLLVEEQLMWQQYAR 583
           LPA P    G A + +S    DPFAAS+ VAPP+YVQM++ME+KQ++L+EEQ+MW QY+R
Sbjct: 541 LPA-PSTANGNAGNINSPVPMDPFAASLEVAPPAYVQMNDMEKKQRMLMEEQMMWDQYSR 580

BLAST of MC00g0056 vs. TAIR 10
Match: AT4G02650.1 (ENTH/ANTH/VHS superfamily protein )

HSP 1 Score: 650.2 bits (1676), Expect = 1.6e-186
Identity = 366/593 (61.72%), Postives = 460/593 (77.57%), Query Frame = 0

Query: 1   MAPSKIRRALGAVKDKTSIGLAKVGS-STSLSDLEVAIVKATRHEEYPAEERHIREILSL 60
           M  SK++RA+GAVKD+TS+GLAKVG  S+SL++LE+A+VKATRH++YPAE+++IREIL L
Sbjct: 1   MGSSKLKRAIGAVKDQTSVGLAKVGGRSSSLTELEIAVVKATRHDDYPAEDKYIREILCL 60

Query: 61  TCYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVLEGDPAYEQEIFFSTRRGTRF 120
           T YSR Y+SACV TLSRRLNKTKNW+VALKTL+LIQRL+ +GD AYEQEIFF+TRRGTR 
Sbjct: 61  TSYSRNYVSACVATLSRRLNKTKNWSVALKTLILIQRLLTDGDRAYEQEIFFATRRGTRL 120

Query: 121 LNMSDFRDVSQSDSWDYSAFVRTYALYLDERLEYRMQSRRGKRSAFGIEDEEEQNNQNNI 180
           LNMSDFRD SQSDSWDYSAFVRTYALYLDERL+YRMQ RRGK+ + G    +  + + + 
Sbjct: 121 LNMSDFRDASQSDSWDYSAFVRTYALYLDERLDYRMQGRRGKKKSGGGGGGDGDSGEED- 180

Query: 181 NNIDTNNNNNSQAIVVRAAPVQEMTTEQIFSKTQHLQQLLERFLACRPTGAAKNNRVVLV 240
           ++  T+N+  S+AIVV++ PV EM TE+IF++ QHLQQLL+RFLACRPTG AKNNRVV+V
Sbjct: 181 DHRGTSNDIRSKAIVVKSKPVAEMKTEKIFNRVQHLQQLLDRFLACRPTGNAKNNRVVIV 240

Query: 241 ALYPVVKESFQIYYDITEIMGILIDRFMELNIPDCVKVYEIFCRVGKQFDELDQLYSWCK 300
           A+YP+VKESFQ+YY+ITEIMG+LI+RFMEL+I D +KVYEIFCRV KQFDELD  Y WCK
Sbjct: 241 AMYPIVKESFQLYYNITEIMGVLIERFMELDIHDSIKVYEIFCRVSKQFDELDPFYGWCK 300

Query: 301 TIGIARSSEYPEVEKITPKKLEVMDDFIKDKSALAQCNRDRPNKPLQLQITEQEEEDSKQ 360
            + +ARSSEYPE+EKIT KKL++MD+FI+DKSALA     +  K    +  + EEE+SK 
Sbjct: 301 NMAVARSSEYPELEKITQKKLDLMDEFIRDKSALAA----QTTKSSSKRSNKSEEEESKT 360

Query: 361 EQEPEDQ-DMNAIKALPPPEDFKPEQEPAAIEVKEESEPKEDEKKKDENPMMCDLLNLGE 420
           E   E+Q D+N+IKALP PE  + E+E   +E K++ E  E   ++D+     DLL+L +
Sbjct: 361 EYIQENQEDLNSIKALPAPEQKEEEEEEEKMETKKDVE--EVVSRQDQEG---DLLDLTD 420

Query: 421 DHAIMREENADKLALALFDGSGPKVEALPAPTWQAFNEETSDWETALVQSASNLSHQRAE 480
           +  +      D LALALFDG     E+   P W+AFN+ ++DWET LV+SA+ LS Q++E
Sbjct: 421 EAGVTAGTVGDSLALALFDGV-VGTESASGPGWEAFNDNSADWETDLVRSATRLSGQKSE 480

Query: 481 LGGGFDMLLLDGMYKQPTV------VTGVGSSGSASSMALGSAGRPA--MLALPAPPPPG 540
           LGGGFD LLLDGMY+   V       T  GSSGSASS+A GSAG PA  MLALPAPPP  
Sbjct: 481 LGGGFDTLLLDGMYQYGAVNAAVKTSTAYGSSGSASSVAFGSAGSPAASMLALPAPPPTA 540

Query: 541 EGGASSS-SSDPFAASVAVAPPSYVQMSEMERKQKLLVEEQLMWQQYARDGRQ 583
            G  +S    DPFAAS+ VAPP+YVQM++ME+KQ+LL+EEQ+MW QY R GRQ
Sbjct: 541 NGNRNSPVMVDPFAASLEVAPPAYVQMNDMEKKQRLLMEEQIMWDQYNRSGRQ 582

BLAST of MC00g0056 vs. TAIR 10
Match: AT2G25430.1 (epsin N-terminal homology (ENTH) domain-containing protein / clathrin assembly protein-related )

HSP 1 Score: 460.3 bits (1183), Expect = 2.4e-129
Identity = 297/652 (45.55%), Postives = 399/652 (61.20%), Query Frame = 0

Query: 1   MAPSKIRRALGAVKDKTSIGLAKVGSSTSLSDLEVAIVKATRHEEYPAEERHIREILSLT 60
           MAPS IR+A+GAVKD+TSIG+AKV S+ +  DLEVAIVKAT H++ PA E++IREIL+LT
Sbjct: 1   MAPS-IRKAIGAVKDQTSIGIAKVASNMA-PDLEVAIVKATSHDDDPASEKYIREILNLT 60

Query: 61  CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVLEGDPAYEQEIFFSTRRGTRFL 120
             SR YI ACV ++SRRL+KT++W VALK LML+ RL+ EGDP +++EI +STRRGTR L
Sbjct: 61  SLSRGYILACVTSVSRRLSKTRDWVVALKALMLVHRLLNEGDPIFQEEILYSTRRGTRML 120

Query: 121 NMSDFRDVSQSDSWDYSAFVRTYALYLDERLEYRMQSRRGKRSAFGIEDEEEQNNQNNIN 180
           NMSDFRD + S SWD+SAFVRTYA YLD+RLE  +  R+      G+      N+ ++ N
Sbjct: 121 NMSDFRDEAHSSSWDHSAFVRTYAGYLDQRLELALFERKS-----GVSVNSGGNSSHHSN 180

Query: 181 NID-----------------------------TNNNNNSQAIVVRA-------------- 240
           N D                               +NN    +  R+              
Sbjct: 181 NDDRYGRGRDDFRSPPPRSYDYENGGGGGSDFRGDNNGYGGVPKRSRSYGDMTEMGGGGG 240

Query: 241 ----------APVQEMTTEQIFSKTQHLQQLLERFLACRPTGAAKNNRVVLVALYPVVKE 300
                      P++EMT E+IF K  HLQ+LL+RFL+ RPTG AKN+R++L+ALYPVV+E
Sbjct: 241 GGGRDEKKVVTPLREMTPERIFGKMGHLQRLLDRFLSLRPTGLAKNSRMILIALYPVVRE 300

Query: 301 SFQIYYDITEIMGILIDRFMELNIPDCVKVYEIFCRVGKQFDELDQLYSWCKTIGIARSS 360
           SF++Y DI E++ +L+D+F ++   DCVK ++ +    KQ DEL   Y+WCK  G+ARSS
Sbjct: 301 SFKLYADICEVLAVLLDKFFDMEYSDCVKAFDAYASAAKQIDELIAFYNWCKETGVARSS 360

Query: 361 EYPEVEKITPKKLEVMDDFIKDKSALAQCNRDRPNKPLQLQITEQEEEDSKQEQEPEDQD 420
           EYPEV++IT K LE +++F++D++        R   P + +I        ++E EP   D
Sbjct: 361 EYPEVQRITSKLLETLEEFVRDRA-------KRGKSPERKEIEAPPPVVEEEEPEP---D 420

Query: 421 MNAIKALPPPEDFKPEQEPAAIEVKEESEPKEDEKKKDENPMMCDLLNLGEDHAIMREEN 480
           MN IKALPPPE++ P   P       E EP+ ++ +  E     DL+NL ED  +  ++ 
Sbjct: 421 MNEIKALPPPENYTPPPPP-------EPEPQPEKPQFTE-----DLVNLREDE-VTADDQ 480

Query: 481 ADKLALALF---DGSGPKVEALPA----PTWQ--AFNEETSDWETALVQSASNLSHQRAE 540
            +K ALALF    G+  K EA  +      WQ  A     +DWE ALV++ SNL  Q A 
Sbjct: 481 GNKFALALFAGPPGNNGKWEAFSSNGVTSAWQNPAAEPGKADWELALVETTSNLEKQTAA 540

Query: 541 LGGGFDMLLLDGMYKQPTVVTGVGSS----GSASSMALGSAGRP--AMLALPAPPPPGEG 585
           LGGGFD LLL+GMY Q  V   V +S    GSASS+AL   G+    +LALPAP    +G
Sbjct: 541 LGGGFDNLLLNGMYDQGMVRQHVSTSQLTGGSASSVALPLPGKTNNQVLALPAP----DG 600

BLAST of MC00g0056 vs. TAIR 10
Match: AT4G32285.1 (ENTH/ANTH/VHS superfamily protein )

HSP 1 Score: 453.4 bits (1165), Expect = 2.9e-127
Identity = 284/624 (45.51%), Postives = 389/624 (62.34%), Query Frame = 0

Query: 6   IRRALGAVKDKTSIGLAKVGSSTSLSDLEVAIVKATRHEEYPAEERHIREILSLTCYSRA 65
           +R+A+G VKD+TSIG+AKV S+ +  DLEVAIVKAT H++  + +++IREILSLT  SR 
Sbjct: 5   MRKAIGVVKDQTSIGIAKVASNMA-PDLEVAIVKATSHDDDQSSDKYIREILSLTSLSRG 64

Query: 66  YISACVNTLSRRLNKTKNWTVALKTLMLIQRLVLEGDPAYEQEIFFSTRRGTRFLNMSDF 125
           Y+ ACV ++SRRL KT++W VALK LML+ RL+ EGDP +++EI ++TRRGTR LNMSDF
Sbjct: 65  YVHACVTSVSRRLKKTRDWIVALKALMLVHRLLNEGDPLFQEEILYATRRGTRILNMSDF 124

Query: 126 RDVSQSDSWDYSAFVRTYALYLDERLEYRMQSRRGKRSAFGIEDEEEQNNQNNIN----- 185
           RD + S SWD+SAFVRTYA YLD+RLE  +  RRG R+  G     + N  +  N     
Sbjct: 125 RDEAHSSSWDHSAFVRTYASYLDQRLELALFERRG-RNGGGSSSSHQSNGDDGYNRSRDD 184

Query: 186 ---------NIDTNNN----------NNSQAIVVR-----AAPVQEMTTEQIFSKTQHLQ 245
                    + +T N            +   I  R       P++EMT E+IF K  HLQ
Sbjct: 185 FRSPPPRTYDYETGNGFGMPKRSRSFGDVNEIGAREEKKSVTPLREMTPERIFGKMGHLQ 244

Query: 246 QLLERFLACRPTGAAKNNRVVLVALYPVVKESFQIYYDITEIMGILIDRFMELNIPDCVK 305
           +LL+RFL+CRPTG AKN+R++L+A+YPVVKESF++Y DI E++ +L+D+F ++   DCVK
Sbjct: 245 RLLDRFLSCRPTGLAKNSRMILIAMYPVVKESFRLYADICEVLAVLLDKFFDMEYTDCVK 304

Query: 306 VYEIFCRVGKQFDELDQLYSWCKTIGIARSSEYPEVEKITPKKLEVMDDFIKDKSALAQC 365
            ++ +    KQ DEL   Y WCK  G+ARSSEYPEV++IT K LE +++F++D++  A+ 
Sbjct: 305 AFDAYASAAKQIDELIAFYHWCKDTGVARSSEYPEVQRITSKLLETLEEFVRDRAKRAKS 364

Query: 366 NRDRPNKPLQLQITEQEEEDSKQEQEPEDQDMNAIKALPPPEDFKPEQEPAAIEVKEESE 425
              +          E E   +      E  DMN IKALPPPE+  P   PA        E
Sbjct: 365 PERK----------EIEAPPAPAPPVEEPVDMNEIKALPPPENHTPPPPPA-------PE 424

Query: 426 PKEDEKKKDENPMMCDLLNLGEDHAIMREENADKLALALFDG---SGPKVEALPA----- 485
           PK  + +  +     DL+NL ED  +  ++  +K ALALF G   +  K EA  +     
Sbjct: 425 PKPQQPQVTD-----DLVNLREDD-VSGDDQGNKFALALFAGPPANNGKWEAFSSDNNVT 484

Query: 486 PTWQ--AFNEETSDWETALVQSASNLSHQRAELGGGFDMLLLDGMYKQPTVVTGVGSS-- 545
             WQ  A     +DWE ALV++ASNL HQ+A +GGG D LLL+GMY Q  V   V +S  
Sbjct: 485 SAWQNPAAELGKADWELALVETASNLEHQKAAMGGGLDPLLLNGMYDQGAVRQHVSTSEL 544

Query: 546 --GSASSMALGSAGR--PAMLALPAPPPPGEGGASSSSSDPFAASVAVAPPSYVQMSEME 585
             GS+SS+AL   G+    +LALPAP    +G     + DPFAAS+ + PPSYVQM+EM+
Sbjct: 545 TGGSSSSVALPLPGKVNSHILALPAP----DGTVQKVNQDPFAASLTIPPPSYVQMAEMD 599

BLAST of MC00g0056 vs. TAIR 10
Match: AT4G32285.2 (ENTH/ANTH/VHS superfamily protein )

HSP 1 Score: 453.4 bits (1165), Expect = 2.9e-127
Identity = 284/624 (45.51%), Postives = 389/624 (62.34%), Query Frame = 0

Query: 6   IRRALGAVKDKTSIGLAKVGSSTSLSDLEVAIVKATRHEEYPAEERHIREILSLTCYSRA 65
           +R+A+G VKD+TSIG+AKV S+ +  DLEVAIVKAT H++  + +++IREILSLT  SR 
Sbjct: 5   MRKAIGVVKDQTSIGIAKVASNMA-PDLEVAIVKATSHDDDQSSDKYIREILSLTSLSRG 64

Query: 66  YISACVNTLSRRLNKTKNWTVALKTLMLIQRLVLEGDPAYEQEIFFSTRRGTRFLNMSDF 125
           Y+ ACV ++SRRL KT++W VALK LML+ RL+ EGDP +++EI ++TRRGTR LNMSDF
Sbjct: 65  YVHACVTSVSRRLKKTRDWIVALKALMLVHRLLNEGDPLFQEEILYATRRGTRILNMSDF 124

Query: 126 RDVSQSDSWDYSAFVRTYALYLDERLEYRMQSRRGKRSAFGIEDEEEQNNQNNIN----- 185
           RD + S SWD+SAFVRTYA YLD+RLE  +  RRG R+  G     + N  +  N     
Sbjct: 125 RDEAHSSSWDHSAFVRTYASYLDQRLELALFERRG-RNGGGSSSSHQSNGDDGYNRSRDD 184

Query: 186 ---------NIDTNNN----------NNSQAIVVR-----AAPVQEMTTEQIFSKTQHLQ 245
                    + +T N            +   I  R       P++EMT E+IF K  HLQ
Sbjct: 185 FRSPPPRTYDYETGNGFGMPKRSRSFGDVNEIGAREEKKSVTPLREMTPERIFGKMGHLQ 244

Query: 246 QLLERFLACRPTGAAKNNRVVLVALYPVVKESFQIYYDITEIMGILIDRFMELNIPDCVK 305
           +LL+RFL+CRPTG AKN+R++L+A+YPVVKESF++Y DI E++ +L+D+F ++   DCVK
Sbjct: 245 RLLDRFLSCRPTGLAKNSRMILIAMYPVVKESFRLYADICEVLAVLLDKFFDMEYTDCVK 304

Query: 306 VYEIFCRVGKQFDELDQLYSWCKTIGIARSSEYPEVEKITPKKLEVMDDFIKDKSALAQC 365
            ++ +    KQ DEL   Y WCK  G+ARSSEYPEV++IT K LE +++F++D++  A+ 
Sbjct: 305 AFDAYASAAKQIDELIAFYHWCKDTGVARSSEYPEVQRITSKLLETLEEFVRDRAKRAKS 364

Query: 366 NRDRPNKPLQLQITEQEEEDSKQEQEPEDQDMNAIKALPPPEDFKPEQEPAAIEVKEESE 425
              +          E E   +      E  DMN IKALPPPE+  P   PA        E
Sbjct: 365 PERK----------EIEAPPAPAPPVEEPVDMNEIKALPPPENHTPPPPPA-------PE 424

Query: 426 PKEDEKKKDENPMMCDLLNLGEDHAIMREENADKLALALFDG---SGPKVEALPA----- 485
           PK  + +  +     DL+NL ED  +  ++  +K ALALF G   +  K EA  +     
Sbjct: 425 PKPQQPQVTD-----DLVNLREDD-VSGDDQGNKFALALFAGPPANNGKWEAFSSDNNVT 484

Query: 486 PTWQ--AFNEETSDWETALVQSASNLSHQRAELGGGFDMLLLDGMYKQPTVVTGVGSS-- 545
             WQ  A     +DWE ALV++ASNL HQ+A +GGG D LLL+GMY Q  V   V +S  
Sbjct: 485 SAWQNPAAELGKADWELALVETASNLEHQKAAMGGGLDPLLLNGMYDQGAVRQHVSTSEL 544

Query: 546 --GSASSMALGSAGR--PAMLALPAPPPPGEGGASSSSSDPFAASVAVAPPSYVQMSEME 585
             GS+SS+AL   G+    +LALPAP    +G     + DPFAAS+ + PPSYVQM+EM+
Sbjct: 545 TGGSSSSVALPLPGKVNSHILALPAP----DGTVQKVNQDPFAASLTIPPPSYVQMAEMD 599

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SA651.0e-19063.91Putative clathrin assembly protein At1g03050 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q8GX472.3e-18561.72Putative clathrin assembly protein At4g02650 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q8LF203.3e-12845.55Putative clathrin assembly protein At2g25430 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q8S9J84.1e-12645.51Probable clathrin assembly protein At4g32285 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q9ZVN63.6e-6640.60Clathrin coat assembly protein AP180 OS=Arabidopsis thaliana OX=3702 GN=AP180 PE... [more]
Match NameE-valueIdentityDescription
XP_022154412.10.0100.00putative clathrin assembly protein At1g03050 [Momordica charantia][more]
XP_038888902.10.080.06putative clathrin assembly protein At1g03050 isoform X1 [Benincasa hispida][more]
XP_038888903.10.080.56putative clathrin assembly protein At1g03050 isoform X2 [Benincasa hispida][more]
XP_008455244.10.080.16PREDICTED: putative clathrin assembly protein At1g03050 [Cucumis melo] >KAA00315... [more]
XP_011658768.10.078.62putative clathrin assembly protein At1g03050 [Cucumis sativus] >KGN43681.1 hypot... [more]
Match NameE-valueIdentityDescription
A0A6J1DLL60.0100.00putative clathrin assembly protein At1g03050 OS=Momordica charantia OX=3673 GN=L... [more]
A0A5A7SK490.080.16Putative clathrin assembly protein OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3C1P90.080.16putative clathrin assembly protein At1g03050 OS=Cucumis melo OX=3656 GN=LOC10349... [more]
A0A0A0K7830.078.62ENTH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G058550 PE=4 S... [more]
A0A6J1HJ283.37e-31175.85putative clathrin assembly protein At1g03050 isoform X1 OS=Cucurbita moschata OX... [more]
Match NameE-valueIdentityDescription
AT1G03050.17.4e-19263.91ENTH/ANTH/VHS superfamily protein [more]
AT4G02650.11.6e-18661.72ENTH/ANTH/VHS superfamily protein [more]
AT2G25430.12.4e-12945.55epsin N-terminal homology (ENTH) domain-containing protein / clathrin assembly p... [more]
AT4G32285.12.9e-12745.51ENTH/ANTH/VHS superfamily protein [more]
AT4G32285.22.9e-12745.51ENTH/ANTH/VHS superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013809ENTH domainSMARTSM00273enth_2coord: 31..161
e-value: 1.4E-47
score: 174.1
IPR013809ENTH domainPROSITEPS50942ENTHcoord: 25..161
score: 35.276161
IPR011417AP180 N-terminal homology (ANTH) domainPFAMPF07651ANTHcoord: 32..329
e-value: 3.1E-99
score: 331.5
IPR014712ANTH domain superfamilyGENE3D1.20.58.150ANTH domaincoord: 195..341
e-value: 7.9E-39
score: 134.6
IPR008942ENTH/VHSGENE3D1.25.40.90coord: 2..157
e-value: 7.6E-43
score: 147.7
IPR008942ENTH/VHSSUPERFAMILY48464ENTH/VHS domaincoord: 31..153
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 383..405
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 351..405
NoneNo IPR availablePANTHERPTHR22951CLATHRIN ASSEMBLY PROTEINcoord: 1..584
NoneNo IPR availablePANTHERPTHR22951:SF66BNAC09G00100D PROTEINcoord: 1..584
NoneNo IPR availableCDDcd16987ANTH_N_AP180_plantcoord: 33..154
e-value: 2.26175E-67
score: 212.869
NoneNo IPR availableSUPERFAMILY89009GAT-like domaincoord: 199..329

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC00g0056.1MC00g0056.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0048268 clathrin coat assembly
biological_process GO:0072583 clathrin-dependent endocytosis
biological_process GO:0006900 vesicle budding from membrane
cellular_component GO:0005905 clathrin-coated pit
cellular_component GO:0030136 clathrin-coated vesicle
cellular_component GO:0005794 Golgi apparatus
molecular_function GO:0005545 1-phosphatidylinositol binding
molecular_function GO:0032050 clathrin heavy chain binding
molecular_function GO:0005546 phosphatidylinositol-4,5-bisphosphate binding
molecular_function GO:0000149 SNARE binding
molecular_function GO:0030276 clathrin binding
molecular_function GO:0005543 phospholipid binding