MC00g0010 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC00g0010
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionRING-type E3 ubiquitin transferase
Locationscaffold31: 126975 .. 127928 (-)
RNA-Seq ExpressionMC00g0010
SyntenyMC00g0010
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CGACACCTTCTCGACGACGACGACGTCGACAAAGATCTCAAGAAATTCTGCAATGGATGCCTCTACTACCAGTACTCTCCCGATTCCTGCCCCTCCGATTGCGTCTCCAAATGCCCAAATTCCTGCTCCTACTTCCCAATTCCAGCCCCACCCTCCAAATCCCCCCACAAGCTCTCCAATCTGGTCGCCGCCACCGTCGCCGTCGTCTCCTTCACCTTCCTCATCGTCCTCTGCTACGCAATCTACGCCAGATTCTACTCCGGCCGCCGCAGACGCCCCCCGCCGGCGACCCAGCGCCAGGACTTTCTCGACGAGGAGCAGGGCCCGGTCGTGGACCACCCCATCTGGTACATCCGCACCGTGGGGCTCCCGCCGGCGGCCATCGCCGCCATCGCTGTCTGCAGATTCAAGTCCGGTGAGGGCTTGATCGACGGAACAGAGTGCTCTGTTTGCTTGAGCGAATTCGAAGAGGGCGAAAACTTGCGGCTTCTCCCCAAGTGCAGCCACGCCTTCCACCTCCCCTGTATCGACACGTGGCTCCGATCCCACACCAGTTGCCCCATGTGCCGCGCTCCGATTCTCTTAGCCGCCGTGCCGGAGGAAACCCAGATGGCGGATCTGCAGAATTCTGGCGATTTTGGTGCGGAGGAGAGGAATTCGGGCGATTTGGGGGCGAATTCTGTGAGAGATCAGGAGGAGGAAGATGAAGTTCTTGATTCGGCTGATGATGGAGATTTGGAGCAGAGGGTGGCCGATTTGCAAGCTGTGAGAAGATCGGTTTCGCTGGATTCTGTATCGGCTCTGGGAATCAGCTCTGTTCTGAAAGTGGATTGTTCTTCTGGAGAAGGTGAAATGGGGCAGAAATGGGGTTCTTCTGCTGCCATGAACAGGTCTGTTTCTTGCAGTGGGAAGTTTCTGATGAGAAGTTCCACTCAGAGAAGTTGTGGAACGAAT

mRNA sequence

CGACACCTTCTCGACGACGACGACGTCGACAAAGATCTCAAGAAATTCTGCAATGGATGCCTCTACTACCAGTACTCTCCCGATTCCTGCCCCTCCGATTGCGTCTCCAAATGCCCAAATTCCTGCTCCTACTTCCCAATTCCAGCCCCACCCTCCAAATCCCCCCACAAGCTCTCCAATCTGGTCGCCGCCACCGTCGCCGTCGTCTCCTTCACCTTCCTCATCGTCCTCTGCTACGCAATCTACGCCAGATTCTACTCCGGCCGCCGCAGACGCCCCCCGCCGGCGACCCAGCGCCAGGACTTTCTCGACGAGGAGCAGGGCCCGGTCGTGGACCACCCCATCTGGTACATCCGCACCGTGGGGCTCCCGCCGGCGGCCATCGCCGCCATCGCTGTCTGCAGATTCAAGTCCGGTGAGGGCTTGATCGACGGAACAGAGTGCTCTGTTTGCTTGAGCGAATTCGAAGAGGGCGAAAACTTGCGGCTTCTCCCCAAGTGCAGCCACGCCTTCCACCTCCCCTGTATCGACACGTGGCTCCGATCCCACACCAGTTGCCCCATGTGCCGCGCTCCGATTCTCTTAGCCGCCGTGCCGGAGGAAACCCAGATGGCGGATCTGCAGAATTCTGGCGATTTTGGTGCGGAGGAGAGGAATTCGGGCGATTTGGGGGCGAATTCTGTGAGAGATCAGGAGGAGGAAGATGAAGTTCTTGATTCGGCTGATGATGGAGATTTGGAGCAGAGGGTGGCCGATTTGCAAGCTGTGAGAAGATCGGTTTCGCTGGATTCTGTATCGGCTCTGGGAATCAGCTCTGTTCTGAAAGTGGATTGTTCTTCTGGAGAAGGTGAAATGGGGCAGAAATGGGGTTCTTCTGCTGCCATGAACAGGTCTGTTTCTTGCAGTGGGAAGTTTCTGATGAGAAGTTCCACTCAGAGAAGTTGTGGAACGAAT

Coding sequence (CDS)

CGACACCTTCTCGACGACGACGACGTCGACAAAGATCTCAAGAAATTCTGCAATGGATGCCTCTACTACCAGTACTCTCCCGATTCCTGCCCCTCCGATTGCGTCTCCAAATGCCCAAATTCCTGCTCCTACTTCCCAATTCCAGCCCCACCCTCCAAATCCCCCCACAAGCTCTCCAATCTGGTCGCCGCCACCGTCGCCGTCGTCTCCTTCACCTTCCTCATCGTCCTCTGCTACGCAATCTACGCCAGATTCTACTCCGGCCGCCGCAGACGCCCCCCGCCGGCGACCCAGCGCCAGGACTTTCTCGACGAGGAGCAGGGCCCGGTCGTGGACCACCCCATCTGGTACATCCGCACCGTGGGGCTCCCGCCGGCGGCCATCGCCGCCATCGCTGTCTGCAGATTCAAGTCCGGTGAGGGCTTGATCGACGGAACAGAGTGCTCTGTTTGCTTGAGCGAATTCGAAGAGGGCGAAAACTTGCGGCTTCTCCCCAAGTGCAGCCACGCCTTCCACCTCCCCTGTATCGACACGTGGCTCCGATCCCACACCAGTTGCCCCATGTGCCGCGCTCCGATTCTCTTAGCCGCCGTGCCGGAGGAAACCCAGATGGCGGATCTGCAGAATTCTGGCGATTTTGGTGCGGAGGAGAGGAATTCGGGCGATTTGGGGGCGAATTCTGTGAGAGATCAGGAGGAGGAAGATGAAGTTCTTGATTCGGCTGATGATGGAGATTTGGAGCAGAGGGTGGCCGATTTGCAAGCTGTGAGAAGATCGGTTTCGCTGGATTCTGTATCGGCTCTGGGAATCAGCTCTGTTCTGAAAGTGGATTGTTCTTCTGGAGAAGGTGAAATGGGGCAGAAATGGGGTTCTTCTGCTGCCATGAACAGGTCTGTTTCTTGCAGTGGGAAGTTTCTGATGAGAAGTTCCACTCAGAGAAGTTGTGGAACGAAT

Protein sequence

RHLLDDDDVDKDLKKFCNGCLYYQYSPDSCPSDCVSKCPNSCSYFPIPAPPSKSPHKLSNLVAATVAVVSFTFLIVLCYAIYARFYSGRRRRPPPATQRQDFLDEEQGPVVDHPIWYIRTVGLPPAAIAAIAVCRFKSGEGLIDGTECSVCLSEFEEGENLRLLPKCSHAFHLPCIDTWLRSHTSCPMCRAPILLAAVPEETQMADLQNSGDFGAEERNSGDLGANSVRDQEEEDEVLDSADDGDLEQRVADLQAVRRSVSLDSVSALGISSVLKVDCSSGEGEMGQKWGSSAAMNRSVSCSGKFLMRSSTQRSCGTN
Homology
BLAST of MC00g0010 vs. ExPASy Swiss-Prot
Match: Q9LX93 (E3 ubiquitin-protein ligase RING1 OS=Arabidopsis thaliana OX=3702 GN=ATL55 PE=1 SV=1)

HSP 1 Score: 149.4 bits (376), Expect = 6.7e-35
Identity = 94/230 (40.87%), Postives = 126/230 (54.78%), Query Frame = 0

Query: 45  FPIPAPPSKSPHKLSNLVAATVAVVS----FTFLIVLCY--AIYARFYSGRRRRPPPATQ 104
           +P P PP   P +LS L+  ++ VV     F FL+   Y      R  S     P    Q
Sbjct: 25  YPPPPPPPPPPRELSLLLPTSICVVGSIILFLFLVFFLYLHITQQRRISAASVTPGDTNQ 84

Query: 105 RQDFLDEEQGPVVD-HPIWYIRTVGLPPAAIAAIAVCRFKSGEGLIDGTECSVCLSEFEE 164
           ++D  + E+    D H +W I TVGL  +AI +I V  FK GEG+IDGTECSVCL+EFEE
Sbjct: 85  QEDEDETEERDFSDFHHVWQIPTVGLHRSAINSITVVGFKKGEGIIDGTECSVCLNEFEE 144

Query: 165 GENLRLLPKCSHAFHLPCIDTWLRSHTSCPMCRAPILLAAVP-----EETQMADLQNSGD 224
            E+LRLLPKCSHAFHL CIDTWL SH +CP+CRAP+LL   P     E     D ++S D
Sbjct: 145 DESLRLLPKCSHAFHLNCIDTWLLSHKNCPLCRAPVLLITEPPHQETETNHQPDSESSND 204

Query: 225 FGAEERNSGDLGANSVRDQEEEDEVLDSADDGDLEQRVADLQAVRRSVSL 263
               + +S     +++     + ++ +    G +E        VRRS S+
Sbjct: 205 LRGRQDSSRSRRNHNIFLPRAQSDLANYCGSGRVEN-------VRRSFSI 247

BLAST of MC00g0010 vs. ExPASy Swiss-Prot
Match: Q8LFY8 (RING-H2 finger protein ATL54 OS=Arabidopsis thaliana OX=3702 GN=ATL54 PE=2 SV=2)

HSP 1 Score: 142.1 bits (357), Expect = 1.1e-32
Identity = 94/248 (37.90%), Postives = 133/248 (53.63%), Query Frame = 0

Query: 22  YYQYSPDSCP---SDCVSKCPN-SCSYFPIPAPPSKSPHKLSNLVAATVAVVSFTFLIVL 81
           YY  SP   P   S   S CP+ S S   +P+  S S     +++  T AV++   +++ 
Sbjct: 42  YYTISPQLPPWSSSPQPSPCPSPSISAVYLPSQDSSSSLDAISIITITGAVLA---ILLT 101

Query: 82  CYAIYARFYSGRRRRPPPATQRQD-----------FLDEEQGPVVDHPIWYIRTVGLPPA 141
            + + A+F+S    R    T + D           F D EQ   VDHPIW IRT GL  +
Sbjct: 102 GFFLVAKFFSDSVNRVNQGTYQSDNEDNDTVMEEEFQDREQ---VDHPIWLIRTTGLQQS 161

Query: 142 AIAAIAVCRFKSGEGLIDGTECSVCLSEFEEGENLRLLPKCSHAFHLPCIDTWLRSHTSC 201
            I +I +C +K G+GLI+ T+C VCL+EFEE E+LRLLPKC+HAFH+ CIDTWL SHT+C
Sbjct: 162 IINSITICNYKRGDGLIERTDCPVCLNEFEEDESLRLLPKCNHAFHISCIDTWLSSHTNC 221

Query: 202 PMCRAPILLAAVPEETQMADLQNSGDFGAEERNSGDLGANSVRDQEEEDEVLDSADDGDL 255
           P+CRA I + +V         + SG        SG    N   D+E+  E+ +  D    
Sbjct: 222 PLCRAGIAMISVTTP------RYSGPVEVTPGGSGSHLENDGVDEEDHGEIENRVDSDFK 277

BLAST of MC00g0010 vs. ExPASy Swiss-Prot
Match: Q9LF64 (RING-H2 finger protein ATL52 OS=Arabidopsis thaliana OX=3702 GN=ATL52 PE=2 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 6.3e-25
Identity = 103/330 (31.21%), Postives = 151/330 (45.76%), Query Frame = 0

Query: 23  YQYSPDSCPSDCVSKCPNSCSYFPIPAPP---------SKSPHKLSNLVAATVAVVSFTF 82
           Y    D     C   CP  C Y   P PP         S S    S L+ A + +++   
Sbjct: 11  YDSYNDCSQGICNIYCPQWC-YLIFPPPPPSFFLDDDSSSSSSSFSPLLIALIGILTSAL 70

Query: 83  LIVLCYAIYARFYSGRRRRPPPATQRQDFLDEEQGPVVDHPIWYIRTVGLPPAAIAAIAV 142
           ++V  Y + +++    R     +++  +     +G             GL  + I +I V
Sbjct: 71  ILVSYYTLISKYC--HRHHQTSSSETLNLNHNGEGFFSSTQRISTNGDGLNESMIKSITV 130

Query: 143 CRFKSGEGLIDGTECSVCLSEFEEGENLRLLPKCSHAFHLPCIDTWLRSHTSCPMCRA-- 202
            ++KSG+G +DG++CSVCLSEFEE E+LRLLPKC+HAFHLPCIDTWL+SH++CP+CRA  
Sbjct: 131 YKYKSGDGFVDGSDCSVCLSEFEENESLRLLPKCNHAFHLPCIDTWLKSHSNCPLCRAFV 190

Query: 203 -----PILLAAVPEETQMADLQNSG-----------DFGAEERNSGDLGANSV-RDQEEE 262
                P           +A+  NS            +    E  +GD  +  V  D E  
Sbjct: 191 TGVNNPTASVGQNVSVVVANQSNSAHQTGSVSEINLNLAGYESQTGDFDSVVVIEDLEIG 250

Query: 263 DEVLDSADDGDL-----EQRVADLQAVRRSVSLDSVSALGISSVLKVDCSSGEGEMGQKW 319
               D+  +  L     E +  D   +RRSVSL+S   + I+ VL+ +    EGE G   
Sbjct: 251 SRNSDARSELQLPEERRETKDEDSLPIRRSVSLNSGVVVSIADVLR-EIEDEEGESGGV- 310

BLAST of MC00g0010 vs. ExPASy Swiss-Prot
Match: Q7XLY8 (E3 ubiquitin-protein ligase Os04g0590900 OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0590900 PE=2 SV=2)

HSP 1 Score: 111.3 bits (277), Expect = 2.0e-23
Identity = 86/256 (33.59%), Postives = 117/256 (45.70%), Query Frame = 0

Query: 23  YQYSPDSCPSDCVSKCPNSCSYFPIPAPP----------SKSPHKLSNLVAATVAVVSFT 82
           Y+ + D     C   CP  C YF  P PP            S    S LV A + V++  
Sbjct: 11  YEPTRDCSQGLCSMYCPQWC-YFIFPPPPPFDVAGTSADDSSGPVFSPLVIAIIGVLASA 70

Query: 83  FLIVLCYAIYARF---YSGRRRRPPPATQRQDFLD--------EEQGPVVDHPIWYIR-T 142
           FL+V  Y   +++    S  R R   ++                  G    H  W +   
Sbjct: 71  FLLVSYYTFISKYCGTVSSLRGRVFGSSSGGAAYGGGAGSGGRHGHGQSRSHESWNVSPP 130

Query: 143 VGLPPAAIAAIAVCRFKSGEGLIDGTECSVCLSEFEEGENLRLLPKCSHAFHLPCIDTWL 202
            GL    I  I VC+++ G+G +  T+CSVCL EF +GE+LRLLP+CSHAFH  CIDTWL
Sbjct: 131 SGLDETLINKITVCKYRRGDGFVHTTDCSVCLGEFSDGESLRLLPRCSHAFHQQCIDTWL 190

Query: 203 RSHTSCPMCRAPILLAAV----PEETQMADLQNSGDFGAEERNSGDLGANSVRDQEEEDE 253
           +SH++CP+CRA I    V    PE    A  +  GD   E     D   N   +Q+E   
Sbjct: 191 KSHSNCPLCRANITFVTVGLASPEPEGCAPGETGGDNTHEVVVVMDGLENLCEEQQEAVS 250

BLAST of MC00g0010 vs. ExPASy Swiss-Prot
Match: Q9SRQ8 (RING-H2 finger protein ATL51 OS=Arabidopsis thaliana OX=3702 GN=ATL51 PE=2 SV=2)

HSP 1 Score: 105.1 bits (261), Expect = 1.4e-21
Identity = 102/335 (30.45%), Postives = 157/335 (46.87%), Query Frame = 0

Query: 19  GCLYYQYSPDSCPSDCVSKCPNSCSYFPIPAPPS--------KSPHKLSNLVAATVAVVS 78
           G  Y  Y  D     C   CP  C Y   P PPS         S    S L+ A + +++
Sbjct: 11  GTTYDSYR-DCSQGVCSVYCPQWC-YVIFPPPPSFYLDDEDDSSSSDFSPLLIALIGILA 70

Query: 79  FTFLIVLCYAIYARFYSGRRRRPPPAT--------------QRQDFLDEEQGPVVDHPIW 138
             F++V  Y + ++ Y  RRR    +T              Q  +  +       +  I 
Sbjct: 71  SAFILVSYYTLISK-YCHRRRHNSSSTSAAAINRISSDYTWQGTNNNNNNGATNPNQTIG 130

Query: 139 YIRTVGLPPAAIAAIAVCRFKSGEGLIDGTECSVCLSEFEEGENLRLLPKCSHAFHLPCI 198
                GL  + I +I V +++  +G ++ ++CSVCLSEF+E E+LRLLPKC+HAFH+PCI
Sbjct: 131 GGGGDGLDESLIKSITVYKYRKMDGFVESSDCSVCLSEFQENESLRLLPKCNHAFHVPCI 190

Query: 199 DTWLRSHTSCPMCRAPILLAAVPEETQMADLQ-----NSGDFGAEERNSGDLGANSVRDQ 258
           DTWL+SH++CP+CRA I+ ++  E   + + Q     NS   G +     +L   + R +
Sbjct: 191 DTWLKSHSNCPLCRAFIVTSSAVEIVDLTNQQIVTENNSISTGDDSVVVVNLDLENSRSR 250

Query: 259 EE--------EDEVLDSADDGDLEQRVADLQAVRRSVSL--------DSVSALGISSVLK 311
            E        +   +  + DG+ E+R A L +    VS+        D   + G+ +   
Sbjct: 251 NETVNEGSTPKPPEMQDSRDGE-ERRSASLNSGGGVVSIADILREIEDDEESAGVGTSRW 310

BLAST of MC00g0010 vs. NCBI nr
Match: XP_008455131.1 (PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis melo] >KAA0031432.1 E3 ubiquitin-protein ligase RING1-like [Cucumis melo var. makuwa] >TYK06883.1 E3 ubiquitin-protein ligase RING1-like [Cucumis melo var. makuwa])

HSP 1 Score: 369 bits (947), Expect = 8.15e-124
Identity = 211/363 (58.13%), Postives = 252/363 (69.42%), Query Frame = 0

Query: 1   RHLLDDDDVDKDLKKFCNGCLYYQYSPDSCPSDCVSKCPNSCSYFPIPAPP--------- 60
           R LL  DD D DLKK C  C  + YS  SCPS C+ KC N C Y  IP PP         
Sbjct: 7   RKLLSSDDEDDDLKKLCKDC--FDYSSYSCPSYCLEKCKNYC-YIHIPIPPPDADDINQT 66

Query: 61  --SKSPHKLSNLVAATVAVVSFTFLIVLCYAIYARFYSGRRRR------PPPATQRQ-DF 120
             +KSPHKLSNLV +T+A++SFTFL+VLCYAIYARFYSGRRRR      PPP T  Q +F
Sbjct: 67  SSTKSPHKLSNLVTSTIALLSFTFLLVLCYAIYARFYSGRRRRIRLPDPPPPETHHQHEF 126

Query: 121 LDEEQGPVVDHPIWYIRTVGLPPAAIAAIAVCRFKSGEGLIDGTECSVCLSEFEEGENLR 180
           LDEEQGPV+DHPIWYIRT+GLPPA I AIAVC+FKSGEGLIDGTECSVCLSEFEE E LR
Sbjct: 127 LDEEQGPVLDHPIWYIRTIGLPPAVIDAIAVCKFKSGEGLIDGTECSVCLSEFEEDETLR 186

Query: 181 LLPKCSHAFHLPCIDTWLRSHTSCPMCRAPILLAAV-----------PEETQMADLQNSG 240
           LLPKCSHAFHLPCIDTWLRSHT+CPMCRAP++  +            P ETQ+ +LQ   
Sbjct: 187 LLPKCSHAFHLPCIDTWLRSHTNCPMCRAPVVAQSSAAAASPPPPRPPAETQIGELQR-- 246

Query: 241 DFGAEERNSGDLGANSVRDQEEEDEVLDSAD----DGDLEQRVADLQAVRRSVSLDSVSA 300
               EE +  +LG +S R++EE+++  +  +    D DLE+R +D+Q VRRSVSLDS+SA
Sbjct: 247 ----EEGDGANLGESSARNEEEQEQQHEEEETHFADEDLEERSSDIQPVRRSVSLDSLSA 306

Query: 301 LGISSVL-----KVDCSSG-------EGEMGQKWGSSAAMNRSVSCSGKFLMRSSTQRSC 318
           L ISSV      K+D  +        +   G+ + S+++MNRSVSCSGKFLMRSSTQ + 
Sbjct: 307 LRISSVFANVNEKLDSDAETIQRKGKKSSQGRGYSSTSSMNRSVSCSGKFLMRSSTQINT 360

BLAST of MC00g0010 vs. NCBI nr
Match: XP_038888113.1 (E3 ubiquitin-protein ligase RING1-like isoform X2 [Benincasa hispida])

HSP 1 Score: 354 bits (908), Expect = 3.31e-118
Identity = 207/348 (59.48%), Postives = 240/348 (68.97%), Query Frame = 0

Query: 1   RHLLDDDDVDKDLKKFCNGCLYYQYSPDSCPSDCVSKCPNSCSY-FPIPAPPSKSPHKLS 60
           R LL DD+   D++KFC  C  ++Y   SC SDC+ KCP  C     IP PPSKSPHKLS
Sbjct: 7   RKLLSDDE--DDIEKFCKDC--HEYLTGSCSSDCIEKCPKLCYISMSIPPPPSKSPHKLS 66

Query: 61  NLVAATVAVVSFTFLIVLCYAIYARFYSGRRRR------PPPATQRQ-DFLDEEQGPVVD 120
           NLV +T+A++SFTFL+VLCYAIYARFYSGRRRR      PPP T  Q DFLDEEQGPV+D
Sbjct: 67  NLVTSTIALLSFTFLLVLCYAIYARFYSGRRRRIRRPDPPPPVTHHQHDFLDEEQGPVLD 126

Query: 121 HPIWYIRTVGLPPAAIAAIAVCRFKSGEGLIDGTECSVCLSEFEEGENLRLLPKCSHAFH 180
           HPIWYIRTVGLPPA I AIAVC+FKSGEGLIDGTECSVCLSEFEE E LRLLPKCSHAFH
Sbjct: 127 HPIWYIRTVGLPPAVIEAIAVCKFKSGEGLIDGTECSVCLSEFEEDETLRLLPKCSHAFH 186

Query: 181 LPCIDTWLRSHTSCPMCRAPILLAAV----------PEETQMADLQNSGDFGAEERNSGD 240
           LPCIDTWLRSHT+CPMCRAP++              P ETQM + Q     GA      +
Sbjct: 187 LPCIDTWLRSHTNCPMCRAPVVAQPTAAASPPPPQPPAETQMREWQRGEGDGA------N 246

Query: 241 LGANSVRDQEEEDEVLD-SADDGDLEQRVADLQAVRRSVSLDSVSALGISSVL-----KV 300
           LG +S R++E+E E ++    + DLE+    +Q VRRS+SLDS+SAL ISSV      K+
Sbjct: 247 LGESSTRNEEQEQEQVEIHFPEEDLEE----IQPVRRSISLDSLSALRISSVFANVNEKM 306

Query: 301 DCSSGEGEMGQKWG------------SSAAMNRSVSCSGKFLMRSSTQ 312
           D    E E  Q+ G            SS+AMNRSVSCSGKFLMR S +
Sbjct: 307 DS---EAESMQRNGNESKKSYQGRGNSSSAMNRSVSCSGKFLMRRSAE 337

BLAST of MC00g0010 vs. NCBI nr
Match: XP_023530563.1 (RING-H2 finger protein ATL54-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 349 bits (896), Expect = 1.36e-116
Identity = 201/344 (58.43%), Postives = 237/344 (68.90%), Query Frame = 0

Query: 1   RHLLDDDDVDKDLKKFC-NGCLYYQYSPDSCPSDCVSKCPNSCSYFPIPAPP---SKSPH 60
           R LL DDD D DLKK C   C    YSPDSCPSDC+ KCPN CS   I  PP    KSPH
Sbjct: 6   RKLLSDDD-DDDLKKLCLKTCP--DYSPDSCPSDCLEKCPNLCSRGSISIPPPYYDKSPH 65

Query: 61  KLSNLVAATVAVVSFTFLIVLCYAIYARFYSGRRRR------PPPATQRQDFLDEEQGPV 120
           KLSN V AT+A +SFTFL+VLCY IY RFYSGRRRR      PPP T +++FLDEE GPV
Sbjct: 66  KLSNWVTATIAFLSFTFLLVLCYTIYVRFYSGRRRRHQRPDPPPPTTHQEEFLDEEHGPV 125

Query: 121 VDHPIWYIRTVGLPPAAIAAIAVCRFKSGEGLIDGTECSVCLSEFEEGENLRLLPKCSHA 180
           +DHPIWYIRT+GLPPA I AIA+C+FK+GEGLIDGTECSVCLSEFEE E LRLLPKCSHA
Sbjct: 126 LDHPIWYIRTIGLPPAVINAIAICKFKTGEGLIDGTECSVCLSEFEEDETLRLLPKCSHA 185

Query: 181 FHLPCIDTWLRSHTSCPMCRAPILL---AAVP------------EETQMADLQNSGDFGA 240
           FHLPCIDTWL+SHT+CPMCRAP++    A  P            EETQM DLQ       
Sbjct: 186 FHLPCIDTWLQSHTNCPMCRAPVVAQSTAVTPPPPPPPPPLVTSEETQMMDLQR------ 245

Query: 241 EERNSGDLGANSVRDQEEEDEVLDSADDGDLEQRVADLQAVRRSVSLDSVSALGI-SSVL 300
           E  N  + G +S+R++E+E++               ++QAVRRSVSLDS+SAL I S + 
Sbjct: 246 EVENGSNSGESSLRNEEQEEQD--------------EIQAVRRSVSLDSLSALRIISDLA 305

Query: 301 KVDCSSGEG----EMGQKWGSSAAMNRSVSCSGKFLMRSSTQRS 314
           ++   +G         ++  SSAAMNRSVSCSG+F+MR+STQ S
Sbjct: 306 EIGMRNGNDLKKKSCQERVNSSAAMNRSVSCSGEFVMRNSTQSS 326

BLAST of MC00g0010 vs. NCBI nr
Match: XP_004137067.1 (RING-H2 finger protein ATL54 [Cucumis sativus] >KGN43773.1 hypothetical protein Csa_017129 [Cucumis sativus])

HSP 1 Score: 349 bits (896), Expect = 4.12e-116
Identity = 203/359 (56.55%), Postives = 241/359 (67.13%), Query Frame = 0

Query: 1   RHLLDDDDVDKDLKKFCNGCLYYQYSPDSCPSDCVSKCPNSCSYFPIPAPPS-------- 60
           R LL +DD D   K+FC  C    Y  DSC SDC+ KC + C    IP  P         
Sbjct: 7   RKLLSEDDDDH--KEFCKDCR--DYFSDSCSSDCLEKCRDYCYNINIPIRPPDADHINPT 66

Query: 61  ---KSPHKLSNLVAATVAVVSFTFLIVLCYAIYARFYSGRRRR------PPPATQRQ-DF 120
              KSPHKLSNLV +T+A++SFTFL+VLCYAIYARFY+GRRRR      PPP T  Q DF
Sbjct: 67  SSIKSPHKLSNLVTSTIALLSFTFLLVLCYAIYARFYAGRRRRIRLPDPPPPETHHQHDF 126

Query: 121 LDEEQGPVVDHPIWYIRTVGLPPAAIAAIAVCRFKSGEGLIDGTECSVCLSEFEEGENLR 180
           LDEEQGPV+DHPIWYIRT+GLPPA I AIAVC+FKSGEGLIDGTECSVCLSEFEE E LR
Sbjct: 127 LDEEQGPVLDHPIWYIRTIGLPPAVIDAIAVCKFKSGEGLIDGTECSVCLSEFEEDETLR 186

Query: 181 LLPKCSHAFHLPCIDTWLRSHTSCPMCRAPILLAAV-----------PEETQMADLQNSG 240
           LLPKCSHAFHLPCIDTWLRSHT+CPMCRAP++  +            P ETQ+ +LQ   
Sbjct: 187 LLPKCSHAFHLPCIDTWLRSHTNCPMCRAPVVAQSAAAAASPPQLRPPAETQIGELQREE 246

Query: 241 DFGAEERNSGDLGANSVRDQEEEDEVLDSADDGDLEQRVADLQAVRRSVSLDSVSALGIS 300
             GA   N G+  A +  +QEE++E      D DLE+R +D+Q +RRSVSLDS+SAL IS
Sbjct: 247 GDGA---NLGESSARTEEEQEEQEEQETHFADEDLEERSSDIQPMRRSVSLDSLSALRIS 306

Query: 301 SVL-----KVDCSSG-------EGEMGQKWGSSAAMNRSVSCSGKFLMRSSTQRSCGTN 318
           S       K+D  +        +   G+   S+++MNRSVSCS KFLMRSSTQ +  +N
Sbjct: 307 SDFANVNEKMDSEAETIQRNGKKSSQGRGHSSTSSMNRSVSCSAKFLMRSSTQINTESN 358

BLAST of MC00g0010 vs. NCBI nr
Match: XP_022988848.1 (RING-H2 finger protein ATL5-like [Cucurbita maxima])

HSP 1 Score: 344 bits (883), Expect = 1.32e-114
Identity = 195/344 (56.69%), Postives = 236/344 (68.60%), Query Frame = 0

Query: 1   RHLLDDDDVDKDLKKFC-NGCLYYQYSPDSCPSDCVSKCPNSCSYFPIPAPP---SKSPH 60
           R LL DDD D D K+ C   C     SPD+CPSDC+ KCPN CS  PI  PP    KSPH
Sbjct: 6   RKLLPDDDDDDDFKELCLKTCP--NNSPDNCPSDCLEKCPNLCSRGPISIPPPYYDKSPH 65

Query: 61  KLSNLVAATVAVVSFTFLIVLCYAIYARFYSGRRRR------PPPATQRQDFLDEEQGPV 120
           KL N V AT+A++SFTFL+VLCY +Y RFYSGRRRR      PPP T +++FLDEE GPV
Sbjct: 66  KLPNWVTATIALLSFTFLLVLCYTVYVRFYSGRRRRRQRPDPPPPTTHQEEFLDEEHGPV 125

Query: 121 VDHPIWYIRTVGLPPAAIAAIAVCRFKSGEGLIDGTECSVCLSEFEEGENLRLLPKCSHA 180
           +DHPIWYIRT+GLPPA I AIA+C+FK+GEGLIDGTECSVCLSEFEE E LRLLPKCSHA
Sbjct: 126 LDHPIWYIRTIGLPPAVINAIAICKFKTGEGLIDGTECSVCLSEFEEDETLRLLPKCSHA 185

Query: 181 FHLPCIDTWLRSHTSCPMCRAPILL---AAVP------------EETQMADLQNSGDFGA 240
           FHLPCIDTWL+SHT+CPMCRAP++    A  P            EETQM DLQ       
Sbjct: 186 FHLPCIDTWLQSHTNCPMCRAPVVAQSTAVTPPPPPPPPPLVTSEETQMRDLQ------M 245

Query: 241 EERNSGDLGANSVRDQEEEDEVLDSADDGDLEQRVADLQAVRRSVSLDSVSALGI-SSVL 300
           E  N  + G +S+R++E++++               ++QAVRRSVSLDS+SAL I S + 
Sbjct: 246 EVGNGSNSGESSLRNEEQQEQD--------------EIQAVRRSVSLDSLSALRIISDLA 305

Query: 301 KVDCSSGEG----EMGQKWGSSAAMNRSVSCSGKFLMRSSTQRS 314
           ++   +G         ++  SSAAMNRSVSCSG+F+MR+STQ S
Sbjct: 306 EIGMRNGNDLKKKSCQERVNSSAAMNRSVSCSGEFVMRNSTQSS 327

BLAST of MC00g0010 vs. ExPASy TrEMBL
Match: A0A5D3C4M8 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G001880 PE=3 SV=1)

HSP 1 Score: 369 bits (947), Expect = 3.95e-124
Identity = 211/363 (58.13%), Postives = 252/363 (69.42%), Query Frame = 0

Query: 1   RHLLDDDDVDKDLKKFCNGCLYYQYSPDSCPSDCVSKCPNSCSYFPIPAPP--------- 60
           R LL  DD D DLKK C  C  + YS  SCPS C+ KC N C Y  IP PP         
Sbjct: 7   RKLLSSDDEDDDLKKLCKDC--FDYSSYSCPSYCLEKCKNYC-YIHIPIPPPDADDINQT 66

Query: 61  --SKSPHKLSNLVAATVAVVSFTFLIVLCYAIYARFYSGRRRR------PPPATQRQ-DF 120
             +KSPHKLSNLV +T+A++SFTFL+VLCYAIYARFYSGRRRR      PPP T  Q +F
Sbjct: 67  SSTKSPHKLSNLVTSTIALLSFTFLLVLCYAIYARFYSGRRRRIRLPDPPPPETHHQHEF 126

Query: 121 LDEEQGPVVDHPIWYIRTVGLPPAAIAAIAVCRFKSGEGLIDGTECSVCLSEFEEGENLR 180
           LDEEQGPV+DHPIWYIRT+GLPPA I AIAVC+FKSGEGLIDGTECSVCLSEFEE E LR
Sbjct: 127 LDEEQGPVLDHPIWYIRTIGLPPAVIDAIAVCKFKSGEGLIDGTECSVCLSEFEEDETLR 186

Query: 181 LLPKCSHAFHLPCIDTWLRSHTSCPMCRAPILLAAV-----------PEETQMADLQNSG 240
           LLPKCSHAFHLPCIDTWLRSHT+CPMCRAP++  +            P ETQ+ +LQ   
Sbjct: 187 LLPKCSHAFHLPCIDTWLRSHTNCPMCRAPVVAQSSAAAASPPPPRPPAETQIGELQR-- 246

Query: 241 DFGAEERNSGDLGANSVRDQEEEDEVLDSAD----DGDLEQRVADLQAVRRSVSLDSVSA 300
               EE +  +LG +S R++EE+++  +  +    D DLE+R +D+Q VRRSVSLDS+SA
Sbjct: 247 ----EEGDGANLGESSARNEEEQEQQHEEEETHFADEDLEERSSDIQPVRRSVSLDSLSA 306

Query: 301 LGISSVL-----KVDCSSG-------EGEMGQKWGSSAAMNRSVSCSGKFLMRSSTQRSC 318
           L ISSV      K+D  +        +   G+ + S+++MNRSVSCSGKFLMRSSTQ + 
Sbjct: 307 LRISSVFANVNEKLDSDAETIQRKGKKSSQGRGYSSTSSMNRSVSCSGKFLMRSSTQINT 360

BLAST of MC00g0010 vs. ExPASy TrEMBL
Match: A0A1S3BZS6 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103495376 PE=3 SV=1)

HSP 1 Score: 369 bits (947), Expect = 3.95e-124
Identity = 211/363 (58.13%), Postives = 252/363 (69.42%), Query Frame = 0

Query: 1   RHLLDDDDVDKDLKKFCNGCLYYQYSPDSCPSDCVSKCPNSCSYFPIPAPP--------- 60
           R LL  DD D DLKK C  C  + YS  SCPS C+ KC N C Y  IP PP         
Sbjct: 7   RKLLSSDDEDDDLKKLCKDC--FDYSSYSCPSYCLEKCKNYC-YIHIPIPPPDADDINQT 66

Query: 61  --SKSPHKLSNLVAATVAVVSFTFLIVLCYAIYARFYSGRRRR------PPPATQRQ-DF 120
             +KSPHKLSNLV +T+A++SFTFL+VLCYAIYARFYSGRRRR      PPP T  Q +F
Sbjct: 67  SSTKSPHKLSNLVTSTIALLSFTFLLVLCYAIYARFYSGRRRRIRLPDPPPPETHHQHEF 126

Query: 121 LDEEQGPVVDHPIWYIRTVGLPPAAIAAIAVCRFKSGEGLIDGTECSVCLSEFEEGENLR 180
           LDEEQGPV+DHPIWYIRT+GLPPA I AIAVC+FKSGEGLIDGTECSVCLSEFEE E LR
Sbjct: 127 LDEEQGPVLDHPIWYIRTIGLPPAVIDAIAVCKFKSGEGLIDGTECSVCLSEFEEDETLR 186

Query: 181 LLPKCSHAFHLPCIDTWLRSHTSCPMCRAPILLAAV-----------PEETQMADLQNSG 240
           LLPKCSHAFHLPCIDTWLRSHT+CPMCRAP++  +            P ETQ+ +LQ   
Sbjct: 187 LLPKCSHAFHLPCIDTWLRSHTNCPMCRAPVVAQSSAAAASPPPPRPPAETQIGELQR-- 246

Query: 241 DFGAEERNSGDLGANSVRDQEEEDEVLDSAD----DGDLEQRVADLQAVRRSVSLDSVSA 300
               EE +  +LG +S R++EE+++  +  +    D DLE+R +D+Q VRRSVSLDS+SA
Sbjct: 247 ----EEGDGANLGESSARNEEEQEQQHEEEETHFADEDLEERSSDIQPVRRSVSLDSLSA 306

Query: 301 LGISSVL-----KVDCSSG-------EGEMGQKWGSSAAMNRSVSCSGKFLMRSSTQRSC 318
           L ISSV      K+D  +        +   G+ + S+++MNRSVSCSGKFLMRSSTQ + 
Sbjct: 307 LRISSVFANVNEKLDSDAETIQRKGKKSSQGRGYSSTSSMNRSVSCSGKFLMRSSTQINT 360

BLAST of MC00g0010 vs. ExPASy TrEMBL
Match: A0A0A0K2P1 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_7G066300 PE=3 SV=1)

HSP 1 Score: 349 bits (896), Expect = 2.00e-116
Identity = 203/359 (56.55%), Postives = 241/359 (67.13%), Query Frame = 0

Query: 1   RHLLDDDDVDKDLKKFCNGCLYYQYSPDSCPSDCVSKCPNSCSYFPIPAPPS-------- 60
           R LL +DD D   K+FC  C    Y  DSC SDC+ KC + C    IP  P         
Sbjct: 7   RKLLSEDDDDH--KEFCKDCR--DYFSDSCSSDCLEKCRDYCYNINIPIRPPDADHINPT 66

Query: 61  ---KSPHKLSNLVAATVAVVSFTFLIVLCYAIYARFYSGRRRR------PPPATQRQ-DF 120
              KSPHKLSNLV +T+A++SFTFL+VLCYAIYARFY+GRRRR      PPP T  Q DF
Sbjct: 67  SSIKSPHKLSNLVTSTIALLSFTFLLVLCYAIYARFYAGRRRRIRLPDPPPPETHHQHDF 126

Query: 121 LDEEQGPVVDHPIWYIRTVGLPPAAIAAIAVCRFKSGEGLIDGTECSVCLSEFEEGENLR 180
           LDEEQGPV+DHPIWYIRT+GLPPA I AIAVC+FKSGEGLIDGTECSVCLSEFEE E LR
Sbjct: 127 LDEEQGPVLDHPIWYIRTIGLPPAVIDAIAVCKFKSGEGLIDGTECSVCLSEFEEDETLR 186

Query: 181 LLPKCSHAFHLPCIDTWLRSHTSCPMCRAPILLAAV-----------PEETQMADLQNSG 240
           LLPKCSHAFHLPCIDTWLRSHT+CPMCRAP++  +            P ETQ+ +LQ   
Sbjct: 187 LLPKCSHAFHLPCIDTWLRSHTNCPMCRAPVVAQSAAAAASPPQLRPPAETQIGELQREE 246

Query: 241 DFGAEERNSGDLGANSVRDQEEEDEVLDSADDGDLEQRVADLQAVRRSVSLDSVSALGIS 300
             GA   N G+  A +  +QEE++E      D DLE+R +D+Q +RRSVSLDS+SAL IS
Sbjct: 247 GDGA---NLGESSARTEEEQEEQEEQETHFADEDLEERSSDIQPMRRSVSLDSLSALRIS 306

Query: 301 SVL-----KVDCSSG-------EGEMGQKWGSSAAMNRSVSCSGKFLMRSSTQRSCGTN 318
           S       K+D  +        +   G+   S+++MNRSVSCS KFLMRSSTQ +  +N
Sbjct: 307 SDFANVNEKMDSEAETIQRNGKKSSQGRGHSSTSSMNRSVSCSAKFLMRSSTQINTESN 358

BLAST of MC00g0010 vs. ExPASy TrEMBL
Match: A0A6J1JNI4 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111486075 PE=3 SV=1)

HSP 1 Score: 344 bits (883), Expect = 6.39e-115
Identity = 195/344 (56.69%), Postives = 236/344 (68.60%), Query Frame = 0

Query: 1   RHLLDDDDVDKDLKKFC-NGCLYYQYSPDSCPSDCVSKCPNSCSYFPIPAPP---SKSPH 60
           R LL DDD D D K+ C   C     SPD+CPSDC+ KCPN CS  PI  PP    KSPH
Sbjct: 6   RKLLPDDDDDDDFKELCLKTCP--NNSPDNCPSDCLEKCPNLCSRGPISIPPPYYDKSPH 65

Query: 61  KLSNLVAATVAVVSFTFLIVLCYAIYARFYSGRRRR------PPPATQRQDFLDEEQGPV 120
           KL N V AT+A++SFTFL+VLCY +Y RFYSGRRRR      PPP T +++FLDEE GPV
Sbjct: 66  KLPNWVTATIALLSFTFLLVLCYTVYVRFYSGRRRRRQRPDPPPPTTHQEEFLDEEHGPV 125

Query: 121 VDHPIWYIRTVGLPPAAIAAIAVCRFKSGEGLIDGTECSVCLSEFEEGENLRLLPKCSHA 180
           +DHPIWYIRT+GLPPA I AIA+C+FK+GEGLIDGTECSVCLSEFEE E LRLLPKCSHA
Sbjct: 126 LDHPIWYIRTIGLPPAVINAIAICKFKTGEGLIDGTECSVCLSEFEEDETLRLLPKCSHA 185

Query: 181 FHLPCIDTWLRSHTSCPMCRAPILL---AAVP------------EETQMADLQNSGDFGA 240
           FHLPCIDTWL+SHT+CPMCRAP++    A  P            EETQM DLQ       
Sbjct: 186 FHLPCIDTWLQSHTNCPMCRAPVVAQSTAVTPPPPPPPPPLVTSEETQMRDLQ------M 245

Query: 241 EERNSGDLGANSVRDQEEEDEVLDSADDGDLEQRVADLQAVRRSVSLDSVSALGI-SSVL 300
           E  N  + G +S+R++E++++               ++QAVRRSVSLDS+SAL I S + 
Sbjct: 246 EVGNGSNSGESSLRNEEQQEQD--------------EIQAVRRSVSLDSLSALRIISDLA 305

Query: 301 KVDCSSGEG----EMGQKWGSSAAMNRSVSCSGKFLMRSSTQRS 314
           ++   +G         ++  SSAAMNRSVSCSG+F+MR+STQ S
Sbjct: 306 EIGMRNGNDLKKKSCQERVNSSAAMNRSVSCSGEFVMRNSTQSS 327

BLAST of MC00g0010 vs. ExPASy TrEMBL
Match: A0A6J1EI25 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111434557 PE=3 SV=1)

HSP 1 Score: 344 bits (883), Expect = 6.84e-115
Identity = 200/345 (57.97%), Postives = 235/345 (68.12%), Query Frame = 0

Query: 1   RHLLDDDDVDKDLKKFC-NGCLYYQYSPDSCPSDCVSKCPNSCSYFPIPAPP---SKSPH 60
           R LL DDD D DLKK C   C     SPDSCPSDC+ KCPN CS   I  PP    KSPH
Sbjct: 6   RKLLSDDD-DDDLKKLCLKTCP--NNSPDSCPSDCLEKCPNLCSRGSISIPPPYYDKSPH 65

Query: 61  KLSNLVAATVAVVSFTFLIVLCYAIYARFYSGRRRR------PPPATQRQDFLDEEQGPV 120
           KLSN V AT+A +SFTFL+V+CY +Y RFYSGRRRR      PPP T +++FLDEE GPV
Sbjct: 66  KLSNWVTATIAFLSFTFLLVICYTVYVRFYSGRRRRHQRPDPPPPTTHQEEFLDEEHGPV 125

Query: 121 VDHPIWYIRTVGLPPAAIAAIAVCRFKSGEGLIDGTECSVCLSEFEEGENLRLLPKCSHA 180
           +DHPIWYIRT+GLPPA I AIA+C+FK+GEGLIDGTECSVCLSEFEE E LRLLPKCSHA
Sbjct: 126 LDHPIWYIRTIGLPPAVINAIAICKFKTGEGLIDGTECSVCLSEFEEDETLRLLPKCSHA 185

Query: 181 FHLPCIDTWLRSHTSCPMCRAPILL---AAVP------------EETQMADLQNSGDFGA 240
           FHLPCIDTWL+SHT+CPMCRAP++    A  P            EETQM DLQ       
Sbjct: 186 FHLPCIDTWLQSHTNCPMCRAPVVAQSTAVTPPPPPPPPPLVTSEETQMRDLQR------ 245

Query: 241 EERNSGDLGANSVRDQEEEDEVLDSADDGDLEQRVADLQAVRRSVSLDSVSALGISSVLK 300
           E  N  + G +S+R++E++++           Q   ++QAVRRSVSLDS+SAL I S L 
Sbjct: 246 EVGNGSNSGESSLRNEEQQEQ-----------QEQDEIQAVRRSVSLDSLSALRIISDL- 305

Query: 301 VDCSSGEGE------MGQKWGSSAAMNRSVSCSGKFLMRSSTQRS 314
            D     G         ++  SSAAMNRSVSCSG+F+MR+STQ S
Sbjct: 306 ADIGMRNGNDLKKKSSQERVNSSAAMNRSVSCSGEFVMRNSTQSS 329

BLAST of MC00g0010 vs. TAIR 10
Match: AT4G33565.1 (RING/U-box superfamily protein )

HSP 1 Score: 179.9 bits (455), Expect = 3.3e-45
Identity = 124/320 (38.75%), Postives = 179/320 (55.94%), Query Frame = 0

Query: 1   RHLLDDDDVDKDLKKFC-NGCLYYQYSPDSCPSDCVSKCPNSCSYFPIP--APPSKSPHK 60
           R LL+D   +      C N C+    +   C S C+ +CP+ C    I   +PP++  H 
Sbjct: 59  RFLLEDMPKETFSDSLCKNNCIQELKTLGYCSSYCLDECPSICEIVVISGLSPPAEIHHD 118

Query: 61  ---LSNLVAATVAVVSFTFLIVLCYAIYARFYSGRRRRPPPATQRQDFLDEEQ-----GP 120
                 L+ +  ++++  FL+ L    +  +Y   RRR    T R+  ++E +     GP
Sbjct: 119 DNLKRILIISASSIITTLFLLTLLVLCFKFYY---RRRRSITTSRRWSMEEARNWDFDGP 178

Query: 121 ---VVDHPIWYIRTVGLPPAAIAAIAVCRFKSGEGLIDGTECSVCLSEFEEGENLRLLPK 180
              +VDHPIW+IRT+GL P  I++I VC++   +G+++GT+CSVCLSEFEE E LRLLPK
Sbjct: 179 APVIVDHPIWHIRTIGLNPTVISSIKVCQYSKKDGVVEGTDCSVCLSEFEEEETLRLLPK 238

Query: 181 CSHAFHLPCIDTWLRSHTSCPMCRAPILLAAVPEETQMADLQNSGDFGAEERNSGDLGAN 240
           C HAFHL CIDTWLRSHT+CP+CRAPI+ A    +     L+       EE  +GD    
Sbjct: 239 CKHAFHLYCIDTWLRSHTNCPLCRAPIVEANTMIDDHSEGLEEISVMIPEE--NGDDTDE 298

Query: 241 SVRDQEEEDEVLDSADDGDLEQRVADLQAVRRSVSLDSVSALGISSVLKVDCSSGEGEMG 300
            + ++ +      S D G+ +Q     Q VRRSVSLDS+S L +S V+ V     + + G
Sbjct: 299 EIEEERDGFVSNISRDHGESQQ-----QRVRRSVSLDSLSGLRVSEVV-VGREKEKLKRG 358

Query: 301 QKWGS--SAAMNRSVSCSGK 305
              GS  S+ + RS+S SGK
Sbjct: 359 NNIGSGRSSLLKRSISYSGK 367

BLAST of MC00g0010 vs. TAIR 10
Match: AT5G10380.1 (RING/U-box superfamily protein )

HSP 1 Score: 149.4 bits (376), Expect = 4.7e-36
Identity = 94/230 (40.87%), Postives = 126/230 (54.78%), Query Frame = 0

Query: 45  FPIPAPPSKSPHKLSNLVAATVAVVS----FTFLIVLCY--AIYARFYSGRRRRPPPATQ 104
           +P P PP   P +LS L+  ++ VV     F FL+   Y      R  S     P    Q
Sbjct: 25  YPPPPPPPPPPRELSLLLPTSICVVGSIILFLFLVFFLYLHITQQRRISAASVTPGDTNQ 84

Query: 105 RQDFLDEEQGPVVD-HPIWYIRTVGLPPAAIAAIAVCRFKSGEGLIDGTECSVCLSEFEE 164
           ++D  + E+    D H +W I TVGL  +AI +I V  FK GEG+IDGTECSVCL+EFEE
Sbjct: 85  QEDEDETEERDFSDFHHVWQIPTVGLHRSAINSITVVGFKKGEGIIDGTECSVCLNEFEE 144

Query: 165 GENLRLLPKCSHAFHLPCIDTWLRSHTSCPMCRAPILLAAVP-----EETQMADLQNSGD 224
            E+LRLLPKCSHAFHL CIDTWL SH +CP+CRAP+LL   P     E     D ++S D
Sbjct: 145 DESLRLLPKCSHAFHLNCIDTWLLSHKNCPLCRAPVLLITEPPHQETETNHQPDSESSND 204

Query: 225 FGAEERNSGDLGANSVRDQEEEDEVLDSADDGDLEQRVADLQAVRRSVSL 263
               + +S     +++     + ++ +    G +E        VRRS S+
Sbjct: 205 LRGRQDSSRSRRNHNIFLPRAQSDLANYCGSGRVEN-------VRRSFSI 247

BLAST of MC00g0010 vs. TAIR 10
Match: AT1G72220.1 (RING/U-box superfamily protein )

HSP 1 Score: 142.1 bits (357), Expect = 7.6e-34
Identity = 94/248 (37.90%), Postives = 133/248 (53.63%), Query Frame = 0

Query: 22  YYQYSPDSCP---SDCVSKCPN-SCSYFPIPAPPSKSPHKLSNLVAATVAVVSFTFLIVL 81
           YY  SP   P   S   S CP+ S S   +P+  S S     +++  T AV++   +++ 
Sbjct: 42  YYTISPQLPPWSSSPQPSPCPSPSISAVYLPSQDSSSSLDAISIITITGAVLA---ILLT 101

Query: 82  CYAIYARFYSGRRRRPPPATQRQD-----------FLDEEQGPVVDHPIWYIRTVGLPPA 141
            + + A+F+S    R    T + D           F D EQ   VDHPIW IRT GL  +
Sbjct: 102 GFFLVAKFFSDSVNRVNQGTYQSDNEDNDTVMEEEFQDREQ---VDHPIWLIRTTGLQQS 161

Query: 142 AIAAIAVCRFKSGEGLIDGTECSVCLSEFEEGENLRLLPKCSHAFHLPCIDTWLRSHTSC 201
            I +I +C +K G+GLI+ T+C VCL+EFEE E+LRLLPKC+HAFH+ CIDTWL SHT+C
Sbjct: 162 IINSITICNYKRGDGLIERTDCPVCLNEFEEDESLRLLPKCNHAFHISCIDTWLSSHTNC 221

Query: 202 PMCRAPILLAAVPEETQMADLQNSGDFGAEERNSGDLGANSVRDQEEEDEVLDSADDGDL 255
           P+CRA I + +V         + SG        SG    N   D+E+  E+ +  D    
Sbjct: 222 PLCRAGIAMISVTTP------RYSGPVEVTPGGSGSHLENDGVDEEDHGEIENRVDSDFK 277

BLAST of MC00g0010 vs. TAIR 10
Match: AT5G17600.1 (RING/U-box superfamily protein )

HSP 1 Score: 116.3 bits (290), Expect = 4.5e-26
Identity = 103/330 (31.21%), Postives = 151/330 (45.76%), Query Frame = 0

Query: 23  YQYSPDSCPSDCVSKCPNSCSYFPIPAPP---------SKSPHKLSNLVAATVAVVSFTF 82
           Y    D     C   CP  C Y   P PP         S S    S L+ A + +++   
Sbjct: 11  YDSYNDCSQGICNIYCPQWC-YLIFPPPPPSFFLDDDSSSSSSSFSPLLIALIGILTSAL 70

Query: 83  LIVLCYAIYARFYSGRRRRPPPATQRQDFLDEEQGPVVDHPIWYIRTVGLPPAAIAAIAV 142
           ++V  Y + +++    R     +++  +     +G             GL  + I +I V
Sbjct: 71  ILVSYYTLISKYC--HRHHQTSSSETLNLNHNGEGFFSSTQRISTNGDGLNESMIKSITV 130

Query: 143 CRFKSGEGLIDGTECSVCLSEFEEGENLRLLPKCSHAFHLPCIDTWLRSHTSCPMCRA-- 202
            ++KSG+G +DG++CSVCLSEFEE E+LRLLPKC+HAFHLPCIDTWL+SH++CP+CRA  
Sbjct: 131 YKYKSGDGFVDGSDCSVCLSEFEENESLRLLPKCNHAFHLPCIDTWLKSHSNCPLCRAFV 190

Query: 203 -----PILLAAVPEETQMADLQNSG-----------DFGAEERNSGDLGANSV-RDQEEE 262
                P           +A+  NS            +    E  +GD  +  V  D E  
Sbjct: 191 TGVNNPTASVGQNVSVVVANQSNSAHQTGSVSEINLNLAGYESQTGDFDSVVVIEDLEIG 250

Query: 263 DEVLDSADDGDL-----EQRVADLQAVRRSVSLDSVSALGISSVLKVDCSSGEGEMGQKW 319
               D+  +  L     E +  D   +RRSVSL+S   + I+ VL+ +    EGE G   
Sbjct: 251 SRNSDARSELQLPEERRETKDEDSLPIRRSVSLNSGVVVSIADVLR-EIEDEEGESGGV- 310

BLAST of MC00g0010 vs. TAIR 10
Match: AT3G03550.1 (RING/U-box superfamily protein )

HSP 1 Score: 105.1 bits (261), Expect = 1.0e-22
Identity = 102/335 (30.45%), Postives = 157/335 (46.87%), Query Frame = 0

Query: 19  GCLYYQYSPDSCPSDCVSKCPNSCSYFPIPAPPS--------KSPHKLSNLVAATVAVVS 78
           G  Y  Y  D     C   CP  C Y   P PPS         S    S L+ A + +++
Sbjct: 11  GTTYDSYR-DCSQGVCSVYCPQWC-YVIFPPPPSFYLDDEDDSSSSDFSPLLIALIGILA 70

Query: 79  FTFLIVLCYAIYARFYSGRRRRPPPAT--------------QRQDFLDEEQGPVVDHPIW 138
             F++V  Y + ++ Y  RRR    +T              Q  +  +       +  I 
Sbjct: 71  SAFILVSYYTLISK-YCHRRRHNSSSTSAAAINRISSDYTWQGTNNNNNNGATNPNQTIG 130

Query: 139 YIRTVGLPPAAIAAIAVCRFKSGEGLIDGTECSVCLSEFEEGENLRLLPKCSHAFHLPCI 198
                GL  + I +I V +++  +G ++ ++CSVCLSEF+E E+LRLLPKC+HAFH+PCI
Sbjct: 131 GGGGDGLDESLIKSITVYKYRKMDGFVESSDCSVCLSEFQENESLRLLPKCNHAFHVPCI 190

Query: 199 DTWLRSHTSCPMCRAPILLAAVPEETQMADLQ-----NSGDFGAEERNSGDLGANSVRDQ 258
           DTWL+SH++CP+CRA I+ ++  E   + + Q     NS   G +     +L   + R +
Sbjct: 191 DTWLKSHSNCPLCRAFIVTSSAVEIVDLTNQQIVTENNSISTGDDSVVVVNLDLENSRSR 250

Query: 259 EE--------EDEVLDSADDGDLEQRVADLQAVRRSVSL--------DSVSALGISSVLK 311
            E        +   +  + DG+ E+R A L +    VS+        D   + G+ +   
Sbjct: 251 NETVNEGSTPKPPEMQDSRDGE-ERRSASLNSGGGVVSIADILREIEDDEESAGVGTSRW 310

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LX936.7e-3540.87E3 ubiquitin-protein ligase RING1 OS=Arabidopsis thaliana OX=3702 GN=ATL55 PE=1 ... [more]
Q8LFY81.1e-3237.90RING-H2 finger protein ATL54 OS=Arabidopsis thaliana OX=3702 GN=ATL54 PE=2 SV=2[more]
Q9LF646.3e-2531.21RING-H2 finger protein ATL52 OS=Arabidopsis thaliana OX=3702 GN=ATL52 PE=2 SV=1[more]
Q7XLY82.0e-2333.59E3 ubiquitin-protein ligase Os04g0590900 OS=Oryza sativa subsp. japonica OX=3994... [more]
Q9SRQ81.4e-2130.45RING-H2 finger protein ATL51 OS=Arabidopsis thaliana OX=3702 GN=ATL51 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
XP_008455131.18.15e-12458.13PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis melo] >KAA0031432.1 E... [more]
XP_038888113.13.31e-11859.48E3 ubiquitin-protein ligase RING1-like isoform X2 [Benincasa hispida][more]
XP_023530563.11.36e-11658.43RING-H2 finger protein ATL54-like [Cucurbita pepo subsp. pepo][more]
XP_004137067.14.12e-11656.55RING-H2 finger protein ATL54 [Cucumis sativus] >KGN43773.1 hypothetical protein ... [more]
XP_022988848.11.32e-11456.69RING-H2 finger protein ATL5-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A5D3C4M83.95e-12458.13RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3BZS63.95e-12458.13RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103495376 PE=3 ... [more]
A0A0A0K2P12.00e-11656.55RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_7G066300 PE... [more]
A0A6J1JNI46.39e-11556.69RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111486075 P... [more]
A0A6J1EI256.84e-11557.97RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111434557... [more]
Match NameE-valueIdentityDescription
AT4G33565.13.3e-4538.75RING/U-box superfamily protein [more]
AT5G10380.14.7e-3640.87RING/U-box superfamily protein [more]
AT1G72220.17.6e-3437.90RING/U-box superfamily protein [more]
AT5G17600.14.5e-2631.21RING/U-box superfamily protein [more]
AT3G03550.11.0e-2230.45RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 148..189
e-value: 1.7E-7
score: 40.9
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 147..190
e-value: 1.3E-13
score: 51.0
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 148..190
score: 12.872856
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 125..195
e-value: 5.2E-22
score: 78.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 211..244
NoneNo IPR availablePANTHERPTHR45676:SF126RING-H2 FINGER PROTEIN ATL54coord: 28..296
NoneNo IPR availableCDDcd16454RING-H2_PA-TM-RINGcoord: 147..190
e-value: 4.91807E-17
score: 71.5641
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 142..195
IPR044675E3 ubiquitin-protein ligase RING1-likePANTHERPTHR45676RING-H2 FINGER PROTEIN ATL51-RELATEDcoord: 28..296

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC00g0010.1MC00g0010.1mRNA