Lsi11G013100 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi11G013100 vs. ExPASy Swiss-Prot
Match: Q9FEA2 (Glutamate--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=OVA3 PE=1 SV=1)

HSP 1 Score: 762.7 bits (1968), Expect = 2.9e-219
Identity = 400/608 (65.79%), Postives = 450/608 (74.01%), Query Frame = 0

Query: 4   IAGTPWVRIKAFPMLNPPILRR--SNLYFGERCIRKFRRSFSVSASVDKV-DGGEVRVRF 63
           + GTPW+R+++ P L P  LRR  S+L++        RRSF+V A    V +GG VRVRF
Sbjct: 5   VYGTPWLRVRSLPELAPAFLRRRQSSLFYCS------RRSFAVVACSTPVNNGGSVRVRF 64

Query: 64  APSPTGNLHVGGARTALFNYLFARANGGKFVLRIEDTDLERSTRQSEEAVLKDLSWLGID 123
           APSPTGNLHVGGARTALFNYLFAR+ GGKFVLRIEDTDLERSTR+SE AVL+DLSWLG+D
Sbjct: 65  APSPTGNLHVGGARTALFNYLFARSKGGKFVLRIEDTDLERSTRESEAAVLQDLSWLGLD 124

Query: 124 WDEGPNAGGEYGPYRQSERNALYKQYAEKLLESGQVYRCFCSNEELERMKEVAKLKQLPP 183
           WDEGP   G++GPYRQSERNALYKQYAEKLLESG VYRCFCS+EEL +MKE AKLKQLPP
Sbjct: 125 WDEGPGVSGDFGPYRQSERNALYKQYAEKLLESGHVYRCFCSSEELVKMKENAKLKQLPP 184

Query: 184 VYMGKWAGATNEEVREELEKGTPYTYRFRVPQEGSLKINDLIRGEVSSPPVLHHPAYSLC 243
           VY GKWA A++ E+ +ELEKGTP+TYRFRVP+EGSLKINDLIRGE               
Sbjct: 185 VYTGKWATASDAEIEQELEKGTPFTYRFRVPKEGSLKINDLIRGE--------------- 244

Query: 244 SPDCMFSVTGPLPLPPSHGLECVLKVSWNLDTLGDFVIMRSNGQPVYNFCVTVDDATMAI 303
                                    V WNLDTLGDFV+MRSNGQPVYNFCVTVDDATMAI
Sbjct: 245 -------------------------VCWNLDTLGDFVVMRSNGQPVYNFCVTVDDATMAI 304

Query: 304 SHVIRAEEHLPNTLRQALIYKALGFPMPYFAHVSLILAPDRSKLSKRHGATSVGQFREMG 363
           SHVIRAEEHLPNTLRQALIYKAL FPMP FAHVSLILAPDRSKLSKRHGATSVGQ+REMG
Sbjct: 305 SHVIRAEEHLPNTLRQALIYKALKFPMPQFAHVSLILAPDRSKLSKRHGATSVGQYREMG 364

Query: 364 YLPEAMVNYLAL----------------LVEKFSISRVNKSGAVFDSTKLRWMNGQHLRA 423
           YLP+ MVNYLAL                LVEKFSI RVNKSGA+FDSTKLRWMNGQHLRA
Sbjct: 365 YLPQGMVNYLALLGWGDGTENEFFTLEDLVEKFSIERVNKSGAIFDSTKLRWMNGQHLRA 424

Query: 424 LPSEELTKLIGERWKSTGILTESEGPFIEESVQLLKDAIDLVTDADKALSNLLSYPLHTT 483
           LP+E+LTKL+GERWKS GILTESEG F+ E+V+LLKD I+LVTD+DK L NLLSYPLH T
Sbjct: 425 LPNEKLTKLVGERWKSAGILTESEGSFVNEAVELLKDGIELVTDSDKVLLNLLSYPLHAT 484

Query: 484 LTSSEAKPLVEDKLSEFSASLIAAYDSGEILNALEEGPSGWQ------------------ 543
           L S EAKP VEDKL E +ASLIAAYDSGEI +ALEEG   WQ                  
Sbjct: 485 LASPEAKPAVEDKLHEVAASLIAAYDSGEIPSALEEGQGAWQKWVKAFGKSLKRKGKSLF 544

Query: 544 ------------------NIVVLHKARSSGVVAPQAGFVPLNDRFEILRRIDWDAVTKDS 556
                             +IV+++KA S G+V PQAGFV + +RF+ILR IDW+A+ KD 
Sbjct: 545 MPLRVLLTGKLHGPEMGTSIVLIYKAGSPGIVVPQAGFVSMEERFKILREIDWEALNKDE 566

BLAST of Lsi11G013100 vs. ExPASy Swiss-Prot
Match: Q43794 (Glutamate--tRNA ligase, chloroplastic/mitochondrial OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 749.6 bits (1934), Expect = 2.6e-215
Identity = 391/606 (64.52%), Postives = 443/606 (73.10%), Query Frame = 0

Query: 1   MATIAGTPWVRIKAFPMLNPP----ILRRSNLYFGERCIRKFRRSFSVSASVDKVDGGEV 60
           MAT+A  PW R++  P L         R ++ Y    C R  RRSFSV AS    DGG+V
Sbjct: 1   MATLAAAPWFRVRLIPELKNSQSLLYCRGNHSYRQSLCSR--RRSFSVYASAG--DGGDV 60

Query: 61  RVRFAPSPTGNLHVGGARTALFNYLFARANGGKFVLRIEDTDLERSTRQSEEAVLKDLSW 120
           RVRFAPSPTGNLHVGGARTALFNYL+ARA GGKF+LRIEDTDLERST++SEEAVL+DLSW
Sbjct: 61  RVRFAPSPTGNLHVGGARTALFNYLYARAKGGKFILRIEDTDLERSTKESEEAVLRDLSW 120

Query: 121 LGIDWDEGPNAGGEYGPYRQSERNALYKQYAEKLLESGQVYRCFCSNEELERMKEVAKLK 180
           LG  WDEGP  GGEYGPYRQSERNALYKQ+AEKLL+SG VYRCFCSNEELE+MKE+AKLK
Sbjct: 121 LGPAWDEGPGIGGEYGPYRQSERNALYKQFAEKLLQSGHVYRCFCSNEELEKMKEIAKLK 180

Query: 181 QLPPVYMGKWAGATNEEVREELEKGTPYTYRFRVPQEGSLKINDLIRGEVSSPPVLHHPA 240
           QLPPVY G+WA AT EEV EEL KGTPYTYRFRVP+EGSLKI+DLIRGE           
Sbjct: 181 QLPPVYTGRWASATEEEVVEELAKGTPYTYRFRVPKEGSLKIDDLIRGE----------- 240

Query: 241 YSLCSPDCMFSVTGPLPLPPSHGLECVLKVSWNLDTLGDFVIMRSNGQPVYNFCVTVDDA 300
                                        VSWNLDTLGDFVIMRSNGQPVYNFCVTVDDA
Sbjct: 241 -----------------------------VSWNLDTLGDFVIMRSNGQPVYNFCVTVDDA 300

Query: 301 TMAISHVIRAEEHLPNTLRQALIYKALGFPMPYFAHVSLILAPDRSKLSKRHGATSVGQF 360
           TMAISHVIRAEEHLPNTLRQALIYKALGFPMP+FAHVSLILAPDRSKLSKRHGATSVGQF
Sbjct: 301 TMAISHVIRAEEHLPNTLRQALIYKALGFPMPHFAHVSLILAPDRSKLSKRHGATSVGQF 360

Query: 361 REMGYLPEAMVNYLAL----------------LVEKFSISRVNKSGAVFDSTKLRWMNGQ 420
           R+MGYLP+AMVNYLAL                LVEKF+I RVNKSGA+FDSTKLRWMNGQ
Sbjct: 361 RDMGYLPQAMVNYLALLGWGDGTENEFFTLEQLVEKFTIERVNKSGAIFDSTKLRWMNGQ 420

Query: 421 HLRALPSEELTKLIGERWKSTGILTESEGPFIEESVQLLKDAIDLVTDADKALSNLLSYP 480
           HLR+LPSEEL ++IGERWK  GI TES+G FI+++V LLKD IDL+TD++KALS+LLSYP
Sbjct: 421 HLRSLPSEELNRIIGERWKDAGIATESQGIFIQDAVLLLKDGIDLITDSEKALSSLLSYP 480

Query: 481 LHTTLTSSEAKPLVEDKLSEFSASLIAAYDSGEILNALEEGPSGWQ-------------- 540
           L+ TL S+E KP++ED +SE + SL+AAYDSGE+  AL EG  GWQ              
Sbjct: 481 LYETLASAEGKPILEDGVSEVAKSLLAAYDSGELSGALAEGQPGWQKWAKNFGKLLKRKG 540

Query: 541 ----------------------NIVVLHKARSSGVVAPQAGFVPLNDRFEILRRIDWDAV 551
                                   V+L+KA +SG V PQAGFV  ++RF+ILR + W++ 
Sbjct: 541 KSLFMPLRVLLTGKLHGPDIGATTVLLYKAGTSGSVVPQAGFVTFDERFKILREVQWESF 562

BLAST of Lsi11G013100 vs. ExPASy Swiss-Prot
Match: Q43768 (Glutamate--tRNA ligase, chloroplastic/mitochondrial OS=Hordeum vulgare OX=4513 PE=2 SV=1)

HSP 1 Score: 700.7 bits (1807), Expect = 1.4e-200
Identity = 361/577 (62.56%), Postives = 426/577 (73.83%), Query Frame = 0

Query: 36  RKFRRSFSVSASVDKVDGGEVRVRFAPSPTGNLHVGGARTALFNYLFARANGGKFVLRIE 95
           R  RR+ +  AS D    G VRVRFAPSPTGNLHVGGARTALFNYLFAR+ GGKFVLR+E
Sbjct: 24  RLSRRALATRASADSGGSGPVRVRFAPSPTGNLHVGGARTALFNYLFARSRGGKFVLRVE 83

Query: 96  DTDLERSTRQSEEAVLKDLSWLGIDWDEGPNAGGEYGPYRQSERNALYKQYAEKLLESGQ 155
           DTDLERST++SEEAVL DLSWLG+DWDEGP+ GG++GPYRQSERNALYK++A+KL+ESG 
Sbjct: 84  DTDLERSTKKSEEAVLTDLSWLGLDWDEGPDIGGDFGPYRQSERNALYKEHAQKLMESGA 143

Query: 156 VYRCFCSNEELERMKEVAKLKQLPPVYMGKWAGATNEEVREELEKGTPYTYRFRVPQEGS 215
           VYRCFCSNEELE+MKE A   ++PPVYMGKWA A++ EV++ELEKGTPYTYRFRVP+EGS
Sbjct: 144 VYRCFCSNEELEKMKETANRMKIPPVYMGKWATASDAEVQQELEKGTPYTYRFRVPKEGS 203

Query: 216 LKINDLIRGEVSSPPVLHHPAYSLCSPDCMFSVTGPLPLPPSHGLECVLKVSWNLDTLGD 275
           LKINDLIRGE                                        VSWNL+TLGD
Sbjct: 204 LKINDLIRGE----------------------------------------VSWNLNTLGD 263

Query: 276 FVIMRSNGQPVYNFCVTVDDATMAISHVIRAEEHLPNTLRQALIYKALGFPMPYFAHVSL 335
           FVIMRSNGQPVYNFCVTVDDATM ISHVIRAEEHLPNTLRQALIYKALGF MP FAHVSL
Sbjct: 264 FVIMRSNGQPVYNFCVTVDDATMRISHVIRAEEHLPNTLRQALIYKALGFAMPLFAHVSL 323

Query: 336 ILAPDRSKLSKRHGATSVGQFREMGYLPEAMVNYLAL----------------LVEKFSI 395
           ILAPD+SKLSKRHGATSVGQ++EMGYLP+AMVNYLAL                LVEKF+I
Sbjct: 324 ILAPDKSKLSKRHGATSVGQYKEMGYLPQAMVNYLALLGWGDGTENEFFTIDDLVEKFTI 383

Query: 396 SRVNKSGAVFDSTKLRWMNGQHLRALPSEELTKLIGERWKSTGILTESEGPFIEESVQLL 455
            RVNKSGAVFD+TKL+WMNGQHLR+LPS+ L K   ++W+STGIL ESE  F +E+ +LL
Sbjct: 384 DRVNKSGAVFDATKLKWMNGQHLRSLPSDLLIKDFEDQWRSTGILLESESGFAKEAAELL 443

Query: 456 KDAIDLVTDADKALSNLLSYPLHTTLTSSEAKPLVEDKLSEFSASLIAAYDSGEILNALE 515
           K+ IDL+TDAD AL  LLSYPLH TL+S EAK +VEDKLSE ++ LI+AYDSGE+  AL 
Sbjct: 444 KEGIDLITDADAALCKLLSYPLHETLSSDEAKSVVEDKLSEVASGLISAYDSGELDQALA 503

Query: 516 EGPSGWQ------------------------------------NIVVLHKARSSGVVAPQ 559
           EG  GW+                                     ++++HKA +SG VAPQ
Sbjct: 504 EGHDGWKKWVKSFGKTHKRKGKSLFMPLRVLLTGKLHGPAMDSTVILVHKAGTSGAVAPQ 559

BLAST of Lsi11G013100 vs. ExPASy Swiss-Prot
Match: Q0AYF8 (Glutamate--tRNA ligase 1 OS=Syntrophomonas wolfei subsp. wolfei (strain DSM 2245B / Goettingen) OX=335541 GN=gltX1 PE=3 SV=1)

HSP 1 Score: 364.8 bits (935), Expect = 1.8e-99
Identity = 189/402 (47.01%), Postives = 247/402 (61.44%), Query Frame = 0

Query: 56  VRVRFAPSPTGNLHVGGARTALFNYLFARANGGKFVLRIEDTDLERSTRQSEEAVLKDLS 115
           +RVRFAPSPTG LH+GGARTALFN+LFAR NGGKF+LRIEDTD  RS   S + +++ LS
Sbjct: 4   IRVRFAPSPTGALHMGGARTALFNWLFARQNGGKFILRIEDTDFRRSREDSAQGIVEGLS 63

Query: 116 WLGIDWDEGPNAGGEYGPYRQSERNALYKQYAEKLLESGQVYRCFCSNEELERMKEVAKL 175
           WLG+DWDEGP+ GG  GPYRQSER  +Y +Y ++LL+SGQ Y CFCS E+L++ +E A  
Sbjct: 64  WLGLDWDEGPDIGGPLGPYRQSERGDIYSRYLQELLDSGQAYYCFCSPEDLQKEREEAAQ 123

Query: 176 KQLPPVYMGKWAGATNEEVREELEKGTPYTYRFRVPQEGSLKINDLIRGEVSSPPVLHHP 235
           ++    Y G+      EE  E L+ G P   R +VP +G+  + DLIRG+VS    L   
Sbjct: 124 EKRDYKYGGRCKALKPEEASEMLQAGKPAVIRLKVPLDGNTVVPDLIRGDVSFSNAL--- 183

Query: 236 AYSLCSPDCMFSVTGPLPLPPSHGLECVLKVSWNLDTLGDFVIMRSNGQPVYNFCVTVDD 295
                                                  DF+I +S+G P YNF V VDD
Sbjct: 184 -------------------------------------FDDFIIAKSDGWPTYNFAVVVDD 243

Query: 296 ATMAISHVIRAEEHLPNTLRQALIYKALGFPMPYFAHVSLILAPDRSKLSKRHGATSVGQ 355
            +M ISHV+RAEEHL NT RQ LIY+ALG   P FAH+S+ILAPDRSKLSKRHGA SV +
Sbjct: 244 FSMQISHVLRAEEHLSNTPRQLLIYRALGLKEPAFAHLSMILAPDRSKLSKRHGAISVQE 303

Query: 356 FREMGYLPEAMVNYLALL----------------VEKFSISRVNKSGAVFDSTKLRWMNG 415
           F   GYLPEA+VNYLALL                + +FS+  ++KS A++D  KL WMNG
Sbjct: 304 FENQGYLPEALVNYLALLGWSTGKDIDIWSREEMMREFSLEHISKSPAIYDLEKLAWMNG 363

Query: 416 QHLRALPSEELTKLIGERWKSTGILTESEGPFIEESVQLLKD 442
           Q++  L  E L  L+  + +  G L E    + +++V+L+++
Sbjct: 364 QYMMRLDIESLMALVEPQAQQQGWLNEDNFDYFQQAVELVRN 365

BLAST of Lsi11G013100 vs. ExPASy Swiss-Prot
Match: A5D5L8 (Glutamate--tRNA ligase OS=Pelotomaculum thermopropionicum (strain DSM 13744 / JCM 10971 / SI) OX=370438 GN=gltX PE=3 SV=1)

HSP 1 Score: 363.2 bits (931), Expect = 5.2e-99
Identity = 196/400 (49.00%), Postives = 250/400 (62.50%), Query Frame = 0

Query: 56  VRVRFAPSPTGNLHVGGARTALFNYLFARANGGKFVLRIEDTDLERSTRQSEEAVLKDLS 115
           VRVRFAPSPTG LH+GGAR+ALFN+LFAR  GGKF++RIEDTDLERS+RQSEE +L  L 
Sbjct: 2   VRVRFAPSPTGPLHIGGARSALFNWLFARRYGGKFIVRIEDTDLERSSRQSEENILAALR 61

Query: 116 WLGIDWDEGPNAGGEYGPYRQSERNALYKQYAEKLLESGQVYRCFCSNEELERMKEVAKL 175
           WLG+DWDEG +AGG   PYRQ+ER  LY+ YAE LL+SG  YRC+C+ EEL   +E    
Sbjct: 62  WLGLDWDEGVDAGGPCEPYRQTERLHLYRHYAEILLQSGAAYRCYCTEEELAAEREALME 121

Query: 176 KQLPPVYMGKWAGATNEEVREELEKGTPYTYRFRVPQEGSLKINDLIRGEVSSPPVLHHP 235
           K   P Y+G+    T +E      +G     RFRVP++  +++ DL+RG+VS        
Sbjct: 122 KGETPRYLGRCRDLTPQERARLEAEGRKAAVRFRVPRDRVIQVKDLVRGDVS-------- 181

Query: 236 AYSLCSPDCMFSVTGPLPLPPSHGLECVLKVSWNLDTLGDFVIMRSNGQPVYNFCVTVDD 295
                                    EC        + +GDF+IM+S+G P YNF V VDD
Sbjct: 182 ------------------------FEC--------NGMGDFIIMKSDGIPTYNFAVVVDD 241

Query: 296 ATMAISHVIRAEEHLPNTLRQALIYKALGFPMPYFAHVSLILAPDRSKLSKRHGATSVGQ 355
            TMAISHVIRAEEHLPNT RQ L+Y+ALG+  P FAHVSLIL  DRSK+SKRHGAT+V Q
Sbjct: 242 HTMAISHVIRAEEHLPNTPRQILLYQALGWDTPEFAHVSLILGKDRSKMSKRHGATAVEQ 301

Query: 356 FREMGYLPEAMVNYLAL----------------LVEKFSISRVNKSGAVFDSTKLRWMNG 415
           +RE GYLPEA+VN+LAL                L ++FS+ +V+KS AVFD  KL W+NG
Sbjct: 302 YREKGYLPEAVVNFLALLGWSPGGENEVLSIEELKQQFSLEKVSKSPAVFDLDKLNWLNG 361

Query: 416 QHLRALPSEELTKLIGERWKSTGILTESEGPFIEESVQLL 440
            ++R    E + +L     +  G +     P   E V+++
Sbjct: 362 YYIRQSSLERVAELAVPFLEKAGYIRAPLSPEEFERVKMM 361

BLAST of Lsi11G013100 vs. ExPASy TrEMBL
Match: A0A0A0K502 (Glutamyl-tRNA synthetase OS=Cucumis sativus OX=3659 GN=Csa_7G027880 PE=3 SV=1)

HSP 1 Score: 971.8 bits (2511), Expect = 1.2e-279
Identity = 502/613 (81.89%), Postives = 513/613 (83.69%), Query Frame = 0

Query: 1   MATIAGTPWVRIKAFPMLNPPILRRSNLYFGERCIRKFRRSFSVSASVDKVDGGEVRVRF 60
           MATIAGTPWVRIKAFP+LNPPIL RSN YFG RCI KFRRSFSVSASVDKVDGG+VRVRF
Sbjct: 1   MATIAGTPWVRIKAFPILNPPILHRSNFYFGVRCIPKFRRSFSVSASVDKVDGGDVRVRF 60

Query: 61  APSPTGNLHVGGARTALFNYLFARANGGKFVLRIEDTDLERSTRQSEEAVLKDLSWLGID 120
           APSPTGNLHVGGARTALFNYLFARANGGKFVLRIEDTDLERSTRQSEEAVL+DLSWLG+D
Sbjct: 61  APSPTGNLHVGGARTALFNYLFARANGGKFVLRIEDTDLERSTRQSEEAVLRDLSWLGLD 120

Query: 121 WDEGPNAGGEYGPYRQSERNALYKQYAEKLLESGQVYRCFCSNEELERMKEVAKLKQLPP 180
           WDEGPN GGEYGPYRQSERNALYKQYAEKLLESGQVYRCFCSNEELE+MKEVAKLKQLPP
Sbjct: 121 WDEGPNVGGEYGPYRQSERNALYKQYAEKLLESGQVYRCFCSNEELEKMKEVAKLKQLPP 180

Query: 181 VYMGKWAGATNEEVREELEKGTPYTYRFRVPQEGSLKINDLIRGEVSSPPVLHHPAYSLC 240
           VYMGKWA ATNEEVREELEKGTPYT+RFRVPQEGSLKINDLIRGE               
Sbjct: 181 VYMGKWASATNEEVREELEKGTPYTFRFRVPQEGSLKINDLIRGE--------------- 240

Query: 241 SPDCMFSVTGPLPLPPSHGLECVLKVSWNLDTLGDFVIMRSNGQPVYNFCVTVDDATMAI 300
                                    VSWNLDTLGDFVIMRSNGQPVYNFCVTVDDATMAI
Sbjct: 241 -------------------------VSWNLDTLGDFVIMRSNGQPVYNFCVTVDDATMAI 300

Query: 301 SHVIRAEEHLPNTLRQALIYKALGFPMPYFAHVSLILAPDRSKLSKRHGATSVGQFREMG 360
           SHVIRAEEHLPNTLRQALIYKALGFPMPYFAHVSLILAPDRSKLSKRHGATSVGQFREMG
Sbjct: 301 SHVIRAEEHLPNTLRQALIYKALGFPMPYFAHVSLILAPDRSKLSKRHGATSVGQFREMG 360

Query: 361 YLPEAMVNYLAL----------------LVEKFSISRVNKSGAVFDSTKLRWMNGQHLRA 420
           YLPEAMVNYLAL                LVEKFSISRVNKSGAVFDSTKLRWMNGQHLRA
Sbjct: 361 YLPEAMVNYLALLGWGDGTENEFFTLEQLVEKFSISRVNKSGAVFDSTKLRWMNGQHLRA 420

Query: 421 LPSEELTKLIGERWKSTGILTESEGPFIEESVQLLKDAIDLVTDADKALSNLLSYPLHTT 480
           LPSEELTKLIGERWKSTGILTESEGPFIEESVQLLKDAIDLVTDADKALSNLLSYPLHTT
Sbjct: 421 LPSEELTKLIGERWKSTGILTESEGPFIEESVQLLKDAIDLVTDADKALSNLLSYPLHTT 480

Query: 481 LTSSEAKPLVEDKLSEFSASLIAAYDSGEILNALEEGPSGWQ------------------ 540
           LTSSEAKPLVEDKLSEFSASLI AYDSGEILNALEEGPSGWQ                  
Sbjct: 481 LTSSEAKPLVEDKLSEFSASLITAYDSGEILNALEEGPSGWQKWVKSFGKSLKRKGKSLF 540

Query: 541 ------------------NIVVLHKARSSGVVAPQAGFVPLNDRFEILRRIDWDAVTKDS 562
                             +IV+LHKARSSGVVAPQAGFVPLNDRFEILR+IDWDAVTKDS
Sbjct: 541 MPLRLLLTGKIHGPDMGASIVLLHKARSSGVVAPQAGFVPLNDRFEILRQIDWDAVTKDS 573

BLAST of Lsi11G013100 vs. ExPASy TrEMBL
Match: A0A5A7V179 (Glutamyl-tRNA synthetase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold886G00050 PE=3 SV=1)

HSP 1 Score: 959.1 bits (2478), Expect = 7.9e-276
Identity = 497/614 (80.94%), Postives = 509/614 (82.90%), Query Frame = 0

Query: 1   MATIAGTPWVRIKAFPMLNPPILRRSNLYFGERCIRKFRRSFSVSASVDKVD-GGEVRVR 60
           MA +AGTPWVRIKAFP+LNPP L RSN YFGERCI KFRRSFSVSASVDKVD GG VRVR
Sbjct: 1   MAAVAGTPWVRIKAFPILNPPFLHRSNFYFGERCIPKFRRSFSVSASVDKVDGGGGVRVR 60

Query: 61  FAPSPTGNLHVGGARTALFNYLFARANGGKFVLRIEDTDLERSTRQSEEAVLKDLSWLGI 120
           FAPSPTGNLHVGGARTALFNYLFARANGG+FVLRIEDTDLERSTRQSEEAVL+DLSWLG+
Sbjct: 61  FAPSPTGNLHVGGARTALFNYLFARANGGEFVLRIEDTDLERSTRQSEEAVLRDLSWLGL 120

Query: 121 DWDEGPNAGGEYGPYRQSERNALYKQYAEKLLESGQVYRCFCSNEELERMKEVAKLKQLP 180
           DWDEGPN GGEYGPYRQSERNALYKQYAEKLLESGQVYRCFCSNEELERMKEVAKL QLP
Sbjct: 121 DWDEGPNVGGEYGPYRQSERNALYKQYAEKLLESGQVYRCFCSNEELERMKEVAKLNQLP 180

Query: 181 PVYMGKWAGATNEEVREELEKGTPYTYRFRVPQEGSLKINDLIRGEVSSPPVLHHPAYSL 240
           PVYMGKWA ATNEEVREELEKGTPYTYRFRVPQEGSLKINDLIRGE              
Sbjct: 181 PVYMGKWASATNEEVREELEKGTPYTYRFRVPQEGSLKINDLIRGE-------------- 240

Query: 241 CSPDCMFSVTGPLPLPPSHGLECVLKVSWNLDTLGDFVIMRSNGQPVYNFCVTVDDATMA 300
                                     VSWNLDTLGDFVIMRSNGQPVYNFCVTVDDATMA
Sbjct: 241 --------------------------VSWNLDTLGDFVIMRSNGQPVYNFCVTVDDATMA 300

Query: 301 ISHVIRAEEHLPNTLRQALIYKALGFPMPYFAHVSLILAPDRSKLSKRHGATSVGQFREM 360
           ISHVIRAEEHLPNTLRQALIYKALGFPMPYFAHVSLILAPDRSKLSKRHGATSVGQFREM
Sbjct: 301 ISHVIRAEEHLPNTLRQALIYKALGFPMPYFAHVSLILAPDRSKLSKRHGATSVGQFREM 360

Query: 361 GYLPEAMVNYLAL----------------LVEKFSISRVNKSGAVFDSTKLRWMNGQHLR 420
           GYLPEAMVNYLAL                LVEKFSISRVNKSGAVFDSTKLRWMNGQHLR
Sbjct: 361 GYLPEAMVNYLALLGWGDGTENEFFTLEQLVEKFSISRVNKSGAVFDSTKLRWMNGQHLR 420

Query: 421 ALPSEELTKLIGERWKSTGILTESEGPFIEESVQLLKDAIDLVTDADKALSNLLSYPLHT 480
           ALPSEELTKLIGERWKSTGILTESEGPF+EESVQLLKDAIDLVTDADKALSNLLSYPLHT
Sbjct: 421 ALPSEELTKLIGERWKSTGILTESEGPFVEESVQLLKDAIDLVTDADKALSNLLSYPLHT 480

Query: 481 TLTSSEAKPLVEDKLSEFSASLIAAYDSGEILNALEEGPSGWQ----------------- 540
           TLTSSEAKPLVEDKLSEFSASLIAAYDSGEILNALEEGPSGWQ                 
Sbjct: 481 TLTSSEAKPLVEDKLSEFSASLIAAYDSGEILNALEEGPSGWQKWVKSFGKSSKRKGKSL 540

Query: 541 -------------------NIVVLHKARSSGVVAPQAGFVPLNDRFEILRRIDWDAVTKD 562
                              +IV+LHKARSSGVVAPQAGFVPLNDRFEILR+IDW+AVT D
Sbjct: 541 FMPLRLLLTGKIHGPDMGASIVLLHKARSSGVVAPQAGFVPLNDRFEILRQIDWNAVTND 574

BLAST of Lsi11G013100 vs. ExPASy TrEMBL
Match: A0A1S3BQW8 (Glutamyl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103492559 PE=3 SV=1)

HSP 1 Score: 958.0 bits (2475), Expect = 1.8e-275
Identity = 496/614 (80.78%), Postives = 507/614 (82.57%), Query Frame = 0

Query: 1   MATIAGTPWVRIKAFPMLNPPILRRSNLYFGERCIRKFRRSFSVSASVDKVD-GGEVRVR 60
           MA +AGTPWVRIKAFP+LNPP L RSN YFGERCI KFRRSFSVSASVDKVD GG VRVR
Sbjct: 1   MAAVAGTPWVRIKAFPILNPPFLHRSNFYFGERCIPKFRRSFSVSASVDKVDGGGGVRVR 60

Query: 61  FAPSPTGNLHVGGARTALFNYLFARANGGKFVLRIEDTDLERSTRQSEEAVLKDLSWLGI 120
           FAPSPTGNLHVGGARTALFNYLFARANGG+FVLRIEDTDLERSTRQSEEAVL+DLSWLG+
Sbjct: 61  FAPSPTGNLHVGGARTALFNYLFARANGGEFVLRIEDTDLERSTRQSEEAVLRDLSWLGL 120

Query: 121 DWDEGPNAGGEYGPYRQSERNALYKQYAEKLLESGQVYRCFCSNEELERMKEVAKLKQLP 180
           DWDEGPN GGEYGPYRQSERNALYKQYAEKLLESGQVYRCFCSNEELERMKEVAKL QLP
Sbjct: 121 DWDEGPNVGGEYGPYRQSERNALYKQYAEKLLESGQVYRCFCSNEELERMKEVAKLNQLP 180

Query: 181 PVYMGKWAGATNEEVREELEKGTPYTYRFRVPQEGSLKINDLIRGEVSSPPVLHHPAYSL 240
           PVYMGKWA ATNEEVREELEKGTPYTYRFRVPQEGSLKINDLIRGE              
Sbjct: 181 PVYMGKWASATNEEVREELEKGTPYTYRFRVPQEGSLKINDLIRGE-------------- 240

Query: 241 CSPDCMFSVTGPLPLPPSHGLECVLKVSWNLDTLGDFVIMRSNGQPVYNFCVTVDDATMA 300
                                     VSWNLDTLGDFVIMRSNGQPVYNFCVTVDD TMA
Sbjct: 241 --------------------------VSWNLDTLGDFVIMRSNGQPVYNFCVTVDDVTMA 300

Query: 301 ISHVIRAEEHLPNTLRQALIYKALGFPMPYFAHVSLILAPDRSKLSKRHGATSVGQFREM 360
           ISHVIRAEEHLPNTLRQALIYKALGFPMPYFAHVSLILAPDRSKLSKRHGATSVGQFREM
Sbjct: 301 ISHVIRAEEHLPNTLRQALIYKALGFPMPYFAHVSLILAPDRSKLSKRHGATSVGQFREM 360

Query: 361 GYLPEAMVNYLAL----------------LVEKFSISRVNKSGAVFDSTKLRWMNGQHLR 420
           GYLPEAMVNYLAL                LVEKFSISRVNKSGAVFDSTKLRWMNGQHLR
Sbjct: 361 GYLPEAMVNYLALLGWGDGTENEFFTLEQLVEKFSISRVNKSGAVFDSTKLRWMNGQHLR 420

Query: 421 ALPSEELTKLIGERWKSTGILTESEGPFIEESVQLLKDAIDLVTDADKALSNLLSYPLHT 480
           ALPSEELTKLIGERWKSTGILTESEGPF+EESVQLLKDAIDLVTDADKALSNLLSYPLHT
Sbjct: 421 ALPSEELTKLIGERWKSTGILTESEGPFVEESVQLLKDAIDLVTDADKALSNLLSYPLHT 480

Query: 481 TLTSSEAKPLVEDKLSEFSASLIAAYDSGEILNALEEGPSGWQ----------------- 540
           TLTSSEAKPLVEDKLSEFSASLIAAYD GEILNALEEGPSGWQ                 
Sbjct: 481 TLTSSEAKPLVEDKLSEFSASLIAAYDGGEILNALEEGPSGWQKWVKSFGKSSKRKGKSL 540

Query: 541 -------------------NIVVLHKARSSGVVAPQAGFVPLNDRFEILRRIDWDAVTKD 562
                              +IV+LHKARSSGVVAPQAGFVPLNDRFEILR+IDWDAVT D
Sbjct: 541 FMPLRLLLTGKIHGPDMGASIVLLHKARSSGVVAPQAGFVPLNDRFEILRQIDWDAVTND 574

BLAST of Lsi11G013100 vs. ExPASy TrEMBL
Match: A0A6J1GMI3 (Glutamyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111455317 PE=3 SV=1)

HSP 1 Score: 939.1 bits (2426), Expect = 8.5e-270
Identity = 486/613 (79.28%), Postives = 506/613 (82.54%), Query Frame = 0

Query: 1   MATIAGTPWVRIKAFPMLNPPILRRSNLYFGERCIRKFRRSFSVSASVDKVDGGEVRVRF 60
           MATI GTPWVRI+AFPMLNPPILR SNLYF ERC  KFRRSFSVSASVDKVD GEVRVRF
Sbjct: 1   MATIVGTPWVRIRAFPMLNPPILRHSNLYFAERCFPKFRRSFSVSASVDKVD-GEVRVRF 60

Query: 61  APSPTGNLHVGGARTALFNYLFARANGGKFVLRIEDTDLERSTRQSEEAVLKDLSWLGID 120
           APSPTGNLHVGGARTALFNYLFARANGGKF+LRIEDTDLERSTRQSEEAVLKDLSWLG+D
Sbjct: 61  APSPTGNLHVGGARTALFNYLFARANGGKFILRIEDTDLERSTRQSEEAVLKDLSWLGLD 120

Query: 121 WDEGPNAGGEYGPYRQSERNALYKQYAEKLLESGQVYRCFCSNEELERMKEVAKLKQLPP 180
           WDEGP+AGG+YGPYRQSERN LYKQYAEKLLESGQVYRCFCSNEELE+MKEVAKLKQLPP
Sbjct: 121 WDEGPSAGGDYGPYRQSERNTLYKQYAEKLLESGQVYRCFCSNEELEKMKEVAKLKQLPP 180

Query: 181 VYMGKWAGATNEEVREELEKGTPYTYRFRVPQEGSLKINDLIRGEVSSPPVLHHPAYSLC 240
           VYMGKWA AT+EE +EELEKGTPYTYRFRVPQEGSLKINDLIRGE               
Sbjct: 181 VYMGKWATATDEEAQEELEKGTPYTYRFRVPQEGSLKINDLIRGE--------------- 240

Query: 241 SPDCMFSVTGPLPLPPSHGLECVLKVSWNLDTLGDFVIMRSNGQPVYNFCVTVDDATMAI 300
                                    VSWNL+TLGDFVIMRSNGQPVYNFCVTVDDATMAI
Sbjct: 241 -------------------------VSWNLNTLGDFVIMRSNGQPVYNFCVTVDDATMAI 300

Query: 301 SHVIRAEEHLPNTLRQALIYKALGFPMPYFAHVSLILAPDRSKLSKRHGATSVGQFREMG 360
           SHVIRAEEHLPNTLRQALIYKALGFPMPYFAHVSLILAPDRSKLSKRHGATSVGQFREMG
Sbjct: 301 SHVIRAEEHLPNTLRQALIYKALGFPMPYFAHVSLILAPDRSKLSKRHGATSVGQFREMG 360

Query: 361 YLPEAMVNYLAL----------------LVEKFSISRVNKSGAVFDSTKLRWMNGQHLRA 420
           YLPEAMVNYLAL                LVEKFSISRVNKSGAVFDSTKLRWMNGQHLRA
Sbjct: 361 YLPEAMVNYLALLGWGDGTENEFFTLEQLVEKFSISRVNKSGAVFDSTKLRWMNGQHLRA 420

Query: 421 LPSEELTKLIGERWKSTGILTESEGPFIEESVQLLKDAIDLVTDADKALSNLLSYPLHTT 480
           LPSEELTKLIGERWKSTG+LTESEGPFIEESV LLKDAIDLVTDADKALSNLLSYPLHTT
Sbjct: 421 LPSEELTKLIGERWKSTGVLTESEGPFIEESVHLLKDAIDLVTDADKALSNLLSYPLHTT 480

Query: 481 LTSSEAKPLVEDKLSEFSASLIAAYDSGEILNALEEGPSGWQ------------------ 540
           LTSSEAKPLVED+LSEFSASLIAAYDSGEIL+ALEEGPSGWQ                  
Sbjct: 481 LTSSEAKPLVEDELSEFSASLIAAYDSGEILSALEEGPSGWQKWVKTFGKSLKRKGKSLF 540

Query: 541 ------------------NIVVLHKARSSGVVAPQAGFVPLNDRFEILRRIDWDAVTKDS 562
                             ++V+LHKA SSGVVAPQ  FV LNDRFEILR+IDWD+VT+DS
Sbjct: 541 MPLRLLLTGKIHGPDMGASMVLLHKAGSSGVVAPQVRFVTLNDRFEILRQIDWDSVTQDS 572

BLAST of Lsi11G013100 vs. ExPASy TrEMBL
Match: A0A6J1HWL8 (Glutamyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111468159 PE=3 SV=1)

HSP 1 Score: 937.6 bits (2422), Expect = 2.5e-269
Identity = 485/613 (79.12%), Postives = 506/613 (82.54%), Query Frame = 0

Query: 1   MATIAGTPWVRIKAFPMLNPPILRRSNLYFGERCIRKFRRSFSVSASVDKVDGGEVRVRF 60
           MATIAGTPWVRI+AFPMLNPPIL  SNLYF ERC  KFRRSFSVSASV+KVD GEVRVRF
Sbjct: 1   MATIAGTPWVRIRAFPMLNPPILHHSNLYFAERCFPKFRRSFSVSASVNKVD-GEVRVRF 60

Query: 61  APSPTGNLHVGGARTALFNYLFARANGGKFVLRIEDTDLERSTRQSEEAVLKDLSWLGID 120
           APSPTGNLHVGGARTALFNYLFAR NGGKFVLRIEDTDLERSTRQSEEAVLKDLSWLG+D
Sbjct: 61  APSPTGNLHVGGARTALFNYLFARGNGGKFVLRIEDTDLERSTRQSEEAVLKDLSWLGLD 120

Query: 121 WDEGPNAGGEYGPYRQSERNALYKQYAEKLLESGQVYRCFCSNEELERMKEVAKLKQLPP 180
           WDEGP+AGG+YGPYRQSERN LYKQYAEKLLESGQVYRCFCSNEELE+MKEVAKLKQLPP
Sbjct: 121 WDEGPSAGGDYGPYRQSERNTLYKQYAEKLLESGQVYRCFCSNEELEKMKEVAKLKQLPP 180

Query: 181 VYMGKWAGATNEEVREELEKGTPYTYRFRVPQEGSLKINDLIRGEVSSPPVLHHPAYSLC 240
           VYMGKWA AT+EE +EELEKGTPYTYRFRVPQ+GSLKINDLIRGE               
Sbjct: 181 VYMGKWATATDEEAQEELEKGTPYTYRFRVPQQGSLKINDLIRGE--------------- 240

Query: 241 SPDCMFSVTGPLPLPPSHGLECVLKVSWNLDTLGDFVIMRSNGQPVYNFCVTVDDATMAI 300
                                    VSWNL+TLGDFVIMRSNGQPVYNFCVTVDDATMAI
Sbjct: 241 -------------------------VSWNLNTLGDFVIMRSNGQPVYNFCVTVDDATMAI 300

Query: 301 SHVIRAEEHLPNTLRQALIYKALGFPMPYFAHVSLILAPDRSKLSKRHGATSVGQFREMG 360
           SHVIRAEEHLPNTLRQALIYKALGFPMPYFAHVSLILAPDRSKLSKRHGATSVGQFREMG
Sbjct: 301 SHVIRAEEHLPNTLRQALIYKALGFPMPYFAHVSLILAPDRSKLSKRHGATSVGQFREMG 360

Query: 361 YLPEAMVNYLAL----------------LVEKFSISRVNKSGAVFDSTKLRWMNGQHLRA 420
           YLPEAMVNYLAL                LVEKFSISRVNKSGAVFDSTKLRWMNGQHLRA
Sbjct: 361 YLPEAMVNYLALLGWGDGTENEFFTLEQLVEKFSISRVNKSGAVFDSTKLRWMNGQHLRA 420

Query: 421 LPSEELTKLIGERWKSTGILTESEGPFIEESVQLLKDAIDLVTDADKALSNLLSYPLHTT 480
           LPSEELTKLIGERWKSTGILTESEGPFIEESVQLLKDAIDL+TDADKALSNLLSYPLHTT
Sbjct: 421 LPSEELTKLIGERWKSTGILTESEGPFIEESVQLLKDAIDLITDADKALSNLLSYPLHTT 480

Query: 481 LTSSEAKPLVEDKLSEFSASLIAAYDSGEILNALEEGPSGWQ------------------ 540
           L SSEAKPLVEDKLSEFSASLIAAYDSG+IL+ALEEGPSGWQ                  
Sbjct: 481 LASSEAKPLVEDKLSEFSASLIAAYDSGKILSALEEGPSGWQKWVKTFGKSLKRKGKSLF 540

Query: 541 ------------------NIVVLHKARSSGVVAPQAGFVPLNDRFEILRRIDWDAVTKDS 562
                             ++V+LHKA SSGVVAPQ GFV LNDRFEILR+IDWD+VT+DS
Sbjct: 541 MPLRLLLTGKIHGPDMGASMVLLHKAGSSGVVAPQVGFVTLNDRFEILRQIDWDSVTQDS 572

BLAST of Lsi11G013100 vs. NCBI nr
Match: XP_038888402.1 (glutamate--tRNA ligase, chloroplastic/mitochondrial [Benincasa hispida])

HSP 1 Score: 981.5 bits (2536), Expect = 3.1e-282
Identity = 511/614 (83.22%), Postives = 517/614 (84.20%), Query Frame = 0

Query: 1   MATIAGTPWVRIKAFPMLNPPILRRSNLYFGERCIRKFRRSFSVSASVDKVDGGEVRVRF 60
           MATIAGTPWVRIKAFPMLNPPIL RSNLYFGERCI KFRRSFSVSASVDKVDGGEVRVRF
Sbjct: 1   MATIAGTPWVRIKAFPMLNPPILHRSNLYFGERCIPKFRRSFSVSASVDKVDGGEVRVRF 60

Query: 61  APSPTGNLHVGGARTALFNYLFARANGGKFVLRIEDTDLERSTRQSEEAVLKDLSWLGID 120
           APSPTGNLHVGGARTALFNYLFARANGGKFVLRIEDTDLERSTRQSEEAVLKDLSWLG+D
Sbjct: 61  APSPTGNLHVGGARTALFNYLFARANGGKFVLRIEDTDLERSTRQSEEAVLKDLSWLGLD 120

Query: 121 WDEGPNAGGEYGPYRQSERNALYKQYAEKLLESGQVYRCFCSNEELERMKEVAKLKQLPP 180
           WDEGPNAGGEYGPYRQSERNALYKQYAEKLLESGQVYRCFCSNEELERMKEVAKLKQLPP
Sbjct: 121 WDEGPNAGGEYGPYRQSERNALYKQYAEKLLESGQVYRCFCSNEELERMKEVAKLKQLPP 180

Query: 181 VYMGKWAGATNEEVREELEKGTPYTYRFRVPQEGSLKINDLIRGEVSSPPVLHHPAYSLC 240
           VYMGKWA ATNEEVREELEKGTPYTYRFRVPQEGSLKINDLIRGE               
Sbjct: 181 VYMGKWASATNEEVREELEKGTPYTYRFRVPQEGSLKINDLIRGE--------------- 240

Query: 241 SPDCMFSVTGPLPLPPSHGLECVLKVSWNLDTLGDFVIMRSNGQPVYNFCVTVDDATMAI 300
                                    VSWNLDTLGDFVIMRSNGQPVYNFCVTVDDATMAI
Sbjct: 241 -------------------------VSWNLDTLGDFVIMRSNGQPVYNFCVTVDDATMAI 300

Query: 301 SHVIRAEEHLPNTLRQALIYKALGFPMPYFAHVSLILAPDRSKLSKRHGATSVGQFREMG 360
           SHVIRAEEHLPNTLRQALIYKALGFPMPYFAHVSLILAPDRSKLSKRHGATSVGQFREMG
Sbjct: 301 SHVIRAEEHLPNTLRQALIYKALGFPMPYFAHVSLILAPDRSKLSKRHGATSVGQFREMG 360

Query: 361 YLPEAMVNYLAL----------------LVEKFSISRVNKSGAVFDSTKLRWMNGQHLRA 420
           YLPEAMVNYLAL                LVEKFSISRVNKSGAVFDSTKLRWMNGQHLRA
Sbjct: 361 YLPEAMVNYLALLGWGDGTENEFFTLEQLVEKFSISRVNKSGAVFDSTKLRWMNGQHLRA 420

Query: 421 LPSEELTKLIGERWKSTGILTESEGPFIEESVQLLKDAIDLVTDADKALSNLLSYPLHTT 480
           LPSEELTKLIGERWKSTGILTESEGPFIEESVQLLKDAIDLVTDADKALSNLLSYPLHTT
Sbjct: 421 LPSEELTKLIGERWKSTGILTESEGPFIEESVQLLKDAIDLVTDADKALSNLLSYPLHTT 480

Query: 481 LTSSEAKPLVEDKLSEFSASLIAAYDSGEILNALEEGPSGWQ------------------ 540
           LTSSEAKPLVEDKLSEFSASLIAAYDSGEILNALEEGPSGWQ                  
Sbjct: 481 LTSSEAKPLVEDKLSEFSASLIAAYDSGEILNALEEGPSGWQKWVKNFGKSLKRKGKSLF 540

Query: 541 ------------------NIVVLHKARSSGVVAPQAGFVPLNDRFEILRRIDWDAVTKDS 562
                             ++V+LHKARSSGVVAPQAGFVPL DRFEILR+IDWDAVTKDS
Sbjct: 541 MPLRLLLTGKIHGPDMGTSVVLLHKARSSGVVAPQAGFVPLTDRFEILRQIDWDAVTKDS 574

BLAST of Lsi11G013100 vs. NCBI nr
Match: XP_004153756.1 (glutamate--tRNA ligase, chloroplastic/mitochondrial [Cucumis sativus] >KGN43372.1 hypothetical protein Csa_020342 [Cucumis sativus])

HSP 1 Score: 971.8 bits (2511), Expect = 2.4e-279
Identity = 502/613 (81.89%), Postives = 513/613 (83.69%), Query Frame = 0

Query: 1   MATIAGTPWVRIKAFPMLNPPILRRSNLYFGERCIRKFRRSFSVSASVDKVDGGEVRVRF 60
           MATIAGTPWVRIKAFP+LNPPIL RSN YFG RCI KFRRSFSVSASVDKVDGG+VRVRF
Sbjct: 1   MATIAGTPWVRIKAFPILNPPILHRSNFYFGVRCIPKFRRSFSVSASVDKVDGGDVRVRF 60

Query: 61  APSPTGNLHVGGARTALFNYLFARANGGKFVLRIEDTDLERSTRQSEEAVLKDLSWLGID 120
           APSPTGNLHVGGARTALFNYLFARANGGKFVLRIEDTDLERSTRQSEEAVL+DLSWLG+D
Sbjct: 61  APSPTGNLHVGGARTALFNYLFARANGGKFVLRIEDTDLERSTRQSEEAVLRDLSWLGLD 120

Query: 121 WDEGPNAGGEYGPYRQSERNALYKQYAEKLLESGQVYRCFCSNEELERMKEVAKLKQLPP 180
           WDEGPN GGEYGPYRQSERNALYKQYAEKLLESGQVYRCFCSNEELE+MKEVAKLKQLPP
Sbjct: 121 WDEGPNVGGEYGPYRQSERNALYKQYAEKLLESGQVYRCFCSNEELEKMKEVAKLKQLPP 180

Query: 181 VYMGKWAGATNEEVREELEKGTPYTYRFRVPQEGSLKINDLIRGEVSSPPVLHHPAYSLC 240
           VYMGKWA ATNEEVREELEKGTPYT+RFRVPQEGSLKINDLIRGE               
Sbjct: 181 VYMGKWASATNEEVREELEKGTPYTFRFRVPQEGSLKINDLIRGE--------------- 240

Query: 241 SPDCMFSVTGPLPLPPSHGLECVLKVSWNLDTLGDFVIMRSNGQPVYNFCVTVDDATMAI 300
                                    VSWNLDTLGDFVIMRSNGQPVYNFCVTVDDATMAI
Sbjct: 241 -------------------------VSWNLDTLGDFVIMRSNGQPVYNFCVTVDDATMAI 300

Query: 301 SHVIRAEEHLPNTLRQALIYKALGFPMPYFAHVSLILAPDRSKLSKRHGATSVGQFREMG 360
           SHVIRAEEHLPNTLRQALIYKALGFPMPYFAHVSLILAPDRSKLSKRHGATSVGQFREMG
Sbjct: 301 SHVIRAEEHLPNTLRQALIYKALGFPMPYFAHVSLILAPDRSKLSKRHGATSVGQFREMG 360

Query: 361 YLPEAMVNYLAL----------------LVEKFSISRVNKSGAVFDSTKLRWMNGQHLRA 420
           YLPEAMVNYLAL                LVEKFSISRVNKSGAVFDSTKLRWMNGQHLRA
Sbjct: 361 YLPEAMVNYLALLGWGDGTENEFFTLEQLVEKFSISRVNKSGAVFDSTKLRWMNGQHLRA 420

Query: 421 LPSEELTKLIGERWKSTGILTESEGPFIEESVQLLKDAIDLVTDADKALSNLLSYPLHTT 480
           LPSEELTKLIGERWKSTGILTESEGPFIEESVQLLKDAIDLVTDADKALSNLLSYPLHTT
Sbjct: 421 LPSEELTKLIGERWKSTGILTESEGPFIEESVQLLKDAIDLVTDADKALSNLLSYPLHTT 480

Query: 481 LTSSEAKPLVEDKLSEFSASLIAAYDSGEILNALEEGPSGWQ------------------ 540
           LTSSEAKPLVEDKLSEFSASLI AYDSGEILNALEEGPSGWQ                  
Sbjct: 481 LTSSEAKPLVEDKLSEFSASLITAYDSGEILNALEEGPSGWQKWVKSFGKSLKRKGKSLF 540

Query: 541 ------------------NIVVLHKARSSGVVAPQAGFVPLNDRFEILRRIDWDAVTKDS 562
                             +IV+LHKARSSGVVAPQAGFVPLNDRFEILR+IDWDAVTKDS
Sbjct: 541 MPLRLLLTGKIHGPDMGASIVLLHKARSSGVVAPQAGFVPLNDRFEILRQIDWDAVTKDS 573

BLAST of Lsi11G013100 vs. NCBI nr
Match: KAA0059595.1 (glutamate--tRNA ligase [Cucumis melo var. makuwa] >TYK08128.1 glutamate--tRNA ligase [Cucumis melo var. makuwa])

HSP 1 Score: 959.1 bits (2478), Expect = 1.6e-275
Identity = 497/614 (80.94%), Postives = 509/614 (82.90%), Query Frame = 0

Query: 1   MATIAGTPWVRIKAFPMLNPPILRRSNLYFGERCIRKFRRSFSVSASVDKVD-GGEVRVR 60
           MA +AGTPWVRIKAFP+LNPP L RSN YFGERCI KFRRSFSVSASVDKVD GG VRVR
Sbjct: 1   MAAVAGTPWVRIKAFPILNPPFLHRSNFYFGERCIPKFRRSFSVSASVDKVDGGGGVRVR 60

Query: 61  FAPSPTGNLHVGGARTALFNYLFARANGGKFVLRIEDTDLERSTRQSEEAVLKDLSWLGI 120
           FAPSPTGNLHVGGARTALFNYLFARANGG+FVLRIEDTDLERSTRQSEEAVL+DLSWLG+
Sbjct: 61  FAPSPTGNLHVGGARTALFNYLFARANGGEFVLRIEDTDLERSTRQSEEAVLRDLSWLGL 120

Query: 121 DWDEGPNAGGEYGPYRQSERNALYKQYAEKLLESGQVYRCFCSNEELERMKEVAKLKQLP 180
           DWDEGPN GGEYGPYRQSERNALYKQYAEKLLESGQVYRCFCSNEELERMKEVAKL QLP
Sbjct: 121 DWDEGPNVGGEYGPYRQSERNALYKQYAEKLLESGQVYRCFCSNEELERMKEVAKLNQLP 180

Query: 181 PVYMGKWAGATNEEVREELEKGTPYTYRFRVPQEGSLKINDLIRGEVSSPPVLHHPAYSL 240
           PVYMGKWA ATNEEVREELEKGTPYTYRFRVPQEGSLKINDLIRGE              
Sbjct: 181 PVYMGKWASATNEEVREELEKGTPYTYRFRVPQEGSLKINDLIRGE-------------- 240

Query: 241 CSPDCMFSVTGPLPLPPSHGLECVLKVSWNLDTLGDFVIMRSNGQPVYNFCVTVDDATMA 300
                                     VSWNLDTLGDFVIMRSNGQPVYNFCVTVDDATMA
Sbjct: 241 --------------------------VSWNLDTLGDFVIMRSNGQPVYNFCVTVDDATMA 300

Query: 301 ISHVIRAEEHLPNTLRQALIYKALGFPMPYFAHVSLILAPDRSKLSKRHGATSVGQFREM 360
           ISHVIRAEEHLPNTLRQALIYKALGFPMPYFAHVSLILAPDRSKLSKRHGATSVGQFREM
Sbjct: 301 ISHVIRAEEHLPNTLRQALIYKALGFPMPYFAHVSLILAPDRSKLSKRHGATSVGQFREM 360

Query: 361 GYLPEAMVNYLAL----------------LVEKFSISRVNKSGAVFDSTKLRWMNGQHLR 420
           GYLPEAMVNYLAL                LVEKFSISRVNKSGAVFDSTKLRWMNGQHLR
Sbjct: 361 GYLPEAMVNYLALLGWGDGTENEFFTLEQLVEKFSISRVNKSGAVFDSTKLRWMNGQHLR 420

Query: 421 ALPSEELTKLIGERWKSTGILTESEGPFIEESVQLLKDAIDLVTDADKALSNLLSYPLHT 480
           ALPSEELTKLIGERWKSTGILTESEGPF+EESVQLLKDAIDLVTDADKALSNLLSYPLHT
Sbjct: 421 ALPSEELTKLIGERWKSTGILTESEGPFVEESVQLLKDAIDLVTDADKALSNLLSYPLHT 480

Query: 481 TLTSSEAKPLVEDKLSEFSASLIAAYDSGEILNALEEGPSGWQ----------------- 540
           TLTSSEAKPLVEDKLSEFSASLIAAYDSGEILNALEEGPSGWQ                 
Sbjct: 481 TLTSSEAKPLVEDKLSEFSASLIAAYDSGEILNALEEGPSGWQKWVKSFGKSSKRKGKSL 540

Query: 541 -------------------NIVVLHKARSSGVVAPQAGFVPLNDRFEILRRIDWDAVTKD 562
                              +IV+LHKARSSGVVAPQAGFVPLNDRFEILR+IDW+AVT D
Sbjct: 541 FMPLRLLLTGKIHGPDMGASIVLLHKARSSGVVAPQAGFVPLNDRFEILRQIDWNAVTND 574

BLAST of Lsi11G013100 vs. NCBI nr
Match: XP_008451188.1 (PREDICTED: glutamate--tRNA ligase, chloroplastic/mitochondrial [Cucumis melo])

HSP 1 Score: 958.0 bits (2475), Expect = 3.6e-275
Identity = 496/614 (80.78%), Postives = 507/614 (82.57%), Query Frame = 0

Query: 1   MATIAGTPWVRIKAFPMLNPPILRRSNLYFGERCIRKFRRSFSVSASVDKVD-GGEVRVR 60
           MA +AGTPWVRIKAFP+LNPP L RSN YFGERCI KFRRSFSVSASVDKVD GG VRVR
Sbjct: 1   MAAVAGTPWVRIKAFPILNPPFLHRSNFYFGERCIPKFRRSFSVSASVDKVDGGGGVRVR 60

Query: 61  FAPSPTGNLHVGGARTALFNYLFARANGGKFVLRIEDTDLERSTRQSEEAVLKDLSWLGI 120
           FAPSPTGNLHVGGARTALFNYLFARANGG+FVLRIEDTDLERSTRQSEEAVL+DLSWLG+
Sbjct: 61  FAPSPTGNLHVGGARTALFNYLFARANGGEFVLRIEDTDLERSTRQSEEAVLRDLSWLGL 120

Query: 121 DWDEGPNAGGEYGPYRQSERNALYKQYAEKLLESGQVYRCFCSNEELERMKEVAKLKQLP 180
           DWDEGPN GGEYGPYRQSERNALYKQYAEKLLESGQVYRCFCSNEELERMKEVAKL QLP
Sbjct: 121 DWDEGPNVGGEYGPYRQSERNALYKQYAEKLLESGQVYRCFCSNEELERMKEVAKLNQLP 180

Query: 181 PVYMGKWAGATNEEVREELEKGTPYTYRFRVPQEGSLKINDLIRGEVSSPPVLHHPAYSL 240
           PVYMGKWA ATNEEVREELEKGTPYTYRFRVPQEGSLKINDLIRGE              
Sbjct: 181 PVYMGKWASATNEEVREELEKGTPYTYRFRVPQEGSLKINDLIRGE-------------- 240

Query: 241 CSPDCMFSVTGPLPLPPSHGLECVLKVSWNLDTLGDFVIMRSNGQPVYNFCVTVDDATMA 300
                                     VSWNLDTLGDFVIMRSNGQPVYNFCVTVDD TMA
Sbjct: 241 --------------------------VSWNLDTLGDFVIMRSNGQPVYNFCVTVDDVTMA 300

Query: 301 ISHVIRAEEHLPNTLRQALIYKALGFPMPYFAHVSLILAPDRSKLSKRHGATSVGQFREM 360
           ISHVIRAEEHLPNTLRQALIYKALGFPMPYFAHVSLILAPDRSKLSKRHGATSVGQFREM
Sbjct: 301 ISHVIRAEEHLPNTLRQALIYKALGFPMPYFAHVSLILAPDRSKLSKRHGATSVGQFREM 360

Query: 361 GYLPEAMVNYLAL----------------LVEKFSISRVNKSGAVFDSTKLRWMNGQHLR 420
           GYLPEAMVNYLAL                LVEKFSISRVNKSGAVFDSTKLRWMNGQHLR
Sbjct: 361 GYLPEAMVNYLALLGWGDGTENEFFTLEQLVEKFSISRVNKSGAVFDSTKLRWMNGQHLR 420

Query: 421 ALPSEELTKLIGERWKSTGILTESEGPFIEESVQLLKDAIDLVTDADKALSNLLSYPLHT 480
           ALPSEELTKLIGERWKSTGILTESEGPF+EESVQLLKDAIDLVTDADKALSNLLSYPLHT
Sbjct: 421 ALPSEELTKLIGERWKSTGILTESEGPFVEESVQLLKDAIDLVTDADKALSNLLSYPLHT 480

Query: 481 TLTSSEAKPLVEDKLSEFSASLIAAYDSGEILNALEEGPSGWQ----------------- 540
           TLTSSEAKPLVEDKLSEFSASLIAAYD GEILNALEEGPSGWQ                 
Sbjct: 481 TLTSSEAKPLVEDKLSEFSASLIAAYDGGEILNALEEGPSGWQKWVKSFGKSSKRKGKSL 540

Query: 541 -------------------NIVVLHKARSSGVVAPQAGFVPLNDRFEILRRIDWDAVTKD 562
                              +IV+LHKARSSGVVAPQAGFVPLNDRFEILR+IDWDAVT D
Sbjct: 541 FMPLRLLLTGKIHGPDMGASIVLLHKARSSGVVAPQAGFVPLNDRFEILRQIDWDAVTND 574

BLAST of Lsi11G013100 vs. NCBI nr
Match: XP_022952700.1 (glutamate--tRNA ligase, chloroplastic/mitochondrial [Cucurbita moschata])

HSP 1 Score: 939.1 bits (2426), Expect = 1.7e-269
Identity = 486/613 (79.28%), Postives = 506/613 (82.54%), Query Frame = 0

Query: 1   MATIAGTPWVRIKAFPMLNPPILRRSNLYFGERCIRKFRRSFSVSASVDKVDGGEVRVRF 60
           MATI GTPWVRI+AFPMLNPPILR SNLYF ERC  KFRRSFSVSASVDKVD GEVRVRF
Sbjct: 1   MATIVGTPWVRIRAFPMLNPPILRHSNLYFAERCFPKFRRSFSVSASVDKVD-GEVRVRF 60

Query: 61  APSPTGNLHVGGARTALFNYLFARANGGKFVLRIEDTDLERSTRQSEEAVLKDLSWLGID 120
           APSPTGNLHVGGARTALFNYLFARANGGKF+LRIEDTDLERSTRQSEEAVLKDLSWLG+D
Sbjct: 61  APSPTGNLHVGGARTALFNYLFARANGGKFILRIEDTDLERSTRQSEEAVLKDLSWLGLD 120

Query: 121 WDEGPNAGGEYGPYRQSERNALYKQYAEKLLESGQVYRCFCSNEELERMKEVAKLKQLPP 180
           WDEGP+AGG+YGPYRQSERN LYKQYAEKLLESGQVYRCFCSNEELE+MKEVAKLKQLPP
Sbjct: 121 WDEGPSAGGDYGPYRQSERNTLYKQYAEKLLESGQVYRCFCSNEELEKMKEVAKLKQLPP 180

Query: 181 VYMGKWAGATNEEVREELEKGTPYTYRFRVPQEGSLKINDLIRGEVSSPPVLHHPAYSLC 240
           VYMGKWA AT+EE +EELEKGTPYTYRFRVPQEGSLKINDLIRGE               
Sbjct: 181 VYMGKWATATDEEAQEELEKGTPYTYRFRVPQEGSLKINDLIRGE--------------- 240

Query: 241 SPDCMFSVTGPLPLPPSHGLECVLKVSWNLDTLGDFVIMRSNGQPVYNFCVTVDDATMAI 300
                                    VSWNL+TLGDFVIMRSNGQPVYNFCVTVDDATMAI
Sbjct: 241 -------------------------VSWNLNTLGDFVIMRSNGQPVYNFCVTVDDATMAI 300

Query: 301 SHVIRAEEHLPNTLRQALIYKALGFPMPYFAHVSLILAPDRSKLSKRHGATSVGQFREMG 360
           SHVIRAEEHLPNTLRQALIYKALGFPMPYFAHVSLILAPDRSKLSKRHGATSVGQFREMG
Sbjct: 301 SHVIRAEEHLPNTLRQALIYKALGFPMPYFAHVSLILAPDRSKLSKRHGATSVGQFREMG 360

Query: 361 YLPEAMVNYLAL----------------LVEKFSISRVNKSGAVFDSTKLRWMNGQHLRA 420
           YLPEAMVNYLAL                LVEKFSISRVNKSGAVFDSTKLRWMNGQHLRA
Sbjct: 361 YLPEAMVNYLALLGWGDGTENEFFTLEQLVEKFSISRVNKSGAVFDSTKLRWMNGQHLRA 420

Query: 421 LPSEELTKLIGERWKSTGILTESEGPFIEESVQLLKDAIDLVTDADKALSNLLSYPLHTT 480
           LPSEELTKLIGERWKSTG+LTESEGPFIEESV LLKDAIDLVTDADKALSNLLSYPLHTT
Sbjct: 421 LPSEELTKLIGERWKSTGVLTESEGPFIEESVHLLKDAIDLVTDADKALSNLLSYPLHTT 480

Query: 481 LTSSEAKPLVEDKLSEFSASLIAAYDSGEILNALEEGPSGWQ------------------ 540
           LTSSEAKPLVED+LSEFSASLIAAYDSGEIL+ALEEGPSGWQ                  
Sbjct: 481 LTSSEAKPLVEDELSEFSASLIAAYDSGEILSALEEGPSGWQKWVKTFGKSLKRKGKSLF 540

Query: 541 ------------------NIVVLHKARSSGVVAPQAGFVPLNDRFEILRRIDWDAVTKDS 562
                             ++V+LHKA SSGVVAPQ  FV LNDRFEILR+IDWD+VT+DS
Sbjct: 541 MPLRLLLTGKIHGPDMGASMVLLHKAGSSGVVAPQVRFVTLNDRFEILRQIDWDSVTQDS 572

BLAST of Lsi11G013100 vs. TAIR 10
Match: AT5G64050.1 (glutamate tRNA synthetase )

HSP 1 Score: 762.7 bits (1968), Expect = 2.1e-220
Identity = 400/608 (65.79%), Postives = 450/608 (74.01%), Query Frame = 0

Query: 4   IAGTPWVRIKAFPMLNPPILRR--SNLYFGERCIRKFRRSFSVSASVDKV-DGGEVRVRF 63
           + GTPW+R+++ P L P  LRR  S+L++        RRSF+V A    V +GG VRVRF
Sbjct: 5   VYGTPWLRVRSLPELAPAFLRRRQSSLFYCS------RRSFAVVACSTPVNNGGSVRVRF 64

Query: 64  APSPTGNLHVGGARTALFNYLFARANGGKFVLRIEDTDLERSTRQSEEAVLKDLSWLGID 123
           APSPTGNLHVGGARTALFNYLFAR+ GGKFVLRIEDTDLERSTR+SE AVL+DLSWLG+D
Sbjct: 65  APSPTGNLHVGGARTALFNYLFARSKGGKFVLRIEDTDLERSTRESEAAVLQDLSWLGLD 124

Query: 124 WDEGPNAGGEYGPYRQSERNALYKQYAEKLLESGQVYRCFCSNEELERMKEVAKLKQLPP 183
           WDEGP   G++GPYRQSERNALYKQYAEKLLESG VYRCFCS+EEL +MKE AKLKQLPP
Sbjct: 125 WDEGPGVSGDFGPYRQSERNALYKQYAEKLLESGHVYRCFCSSEELVKMKENAKLKQLPP 184

Query: 184 VYMGKWAGATNEEVREELEKGTPYTYRFRVPQEGSLKINDLIRGEVSSPPVLHHPAYSLC 243
           VY GKWA A++ E+ +ELEKGTP+TYRFRVP+EGSLKINDLIRGE               
Sbjct: 185 VYTGKWATASDAEIEQELEKGTPFTYRFRVPKEGSLKINDLIRGE--------------- 244

Query: 244 SPDCMFSVTGPLPLPPSHGLECVLKVSWNLDTLGDFVIMRSNGQPVYNFCVTVDDATMAI 303
                                    V WNLDTLGDFV+MRSNGQPVYNFCVTVDDATMAI
Sbjct: 245 -------------------------VCWNLDTLGDFVVMRSNGQPVYNFCVTVDDATMAI 304

Query: 304 SHVIRAEEHLPNTLRQALIYKALGFPMPYFAHVSLILAPDRSKLSKRHGATSVGQFREMG 363
           SHVIRAEEHLPNTLRQALIYKAL FPMP FAHVSLILAPDRSKLSKRHGATSVGQ+REMG
Sbjct: 305 SHVIRAEEHLPNTLRQALIYKALKFPMPQFAHVSLILAPDRSKLSKRHGATSVGQYREMG 364

Query: 364 YLPEAMVNYLAL----------------LVEKFSISRVNKSGAVFDSTKLRWMNGQHLRA 423
           YLP+ MVNYLAL                LVEKFSI RVNKSGA+FDSTKLRWMNGQHLRA
Sbjct: 365 YLPQGMVNYLALLGWGDGTENEFFTLEDLVEKFSIERVNKSGAIFDSTKLRWMNGQHLRA 424

Query: 424 LPSEELTKLIGERWKSTGILTESEGPFIEESVQLLKDAIDLVTDADKALSNLLSYPLHTT 483
           LP+E+LTKL+GERWKS GILTESEG F+ E+V+LLKD I+LVTD+DK L NLLSYPLH T
Sbjct: 425 LPNEKLTKLVGERWKSAGILTESEGSFVNEAVELLKDGIELVTDSDKVLLNLLSYPLHAT 484

Query: 484 LTSSEAKPLVEDKLSEFSASLIAAYDSGEILNALEEGPSGWQ------------------ 543
           L S EAKP VEDKL E +ASLIAAYDSGEI +ALEEG   WQ                  
Sbjct: 485 LASPEAKPAVEDKLHEVAASLIAAYDSGEIPSALEEGQGAWQKWVKAFGKSLKRKGKSLF 544

Query: 544 ------------------NIVVLHKARSSGVVAPQAGFVPLNDRFEILRRIDWDAVTKDS 556
                             +IV+++KA S G+V PQAGFV + +RF+ILR IDW+A+ KD 
Sbjct: 545 MPLRVLLTGKLHGPEMGTSIVLIYKAGSPGIVVPQAGFVSMEERFKILREIDWEALNKDE 566

BLAST of Lsi11G013100 vs. TAIR 10
Match: AT5G26710.1 (Glutamyl/glutaminyl-tRNA synthetase, class Ic )

HSP 1 Score: 63.5 bits (153), Expect = 6.0e-10
Identity = 36/115 (31.30%), Postives = 62/115 (53.91%), Query Frame = 0

Query: 54  GEVRVRFAPSPTGNLHVGGARTALFNYLFARANGGKFVLRIEDTDLERSTRQSEEAVLKD 113
           G+V++RFAP P+G LH+G A+ AL N  FA    G+ ++R +DT+  + + +  + ++KD
Sbjct: 212 GKVKLRFAPEPSGYLHIGHAKAALLNKYFAERYQGEVIVRFDDTNPAKESNEFVDNLVKD 271

Query: 114 LSWLGIDWDEGPNAGGEYGPYRQSERNALYKQYAEKLLESGQVYRCFCSNEELER 169
           +  LGI + E      +Y P             AEKL+  G+ Y      E++++
Sbjct: 272 IGTLGIKY-EKVTYTSDYFPE--------LMDMAEKLMREGKAYVDDTPREQMQK 317

BLAST of Lsi11G013100 vs. TAIR 10
Match: AT1G25350.1 (glutamine-tRNA ligase, putative / glutaminyl-tRNA synthetase, putative / GlnRS, putative )

HSP 1 Score: 58.5 bits (140), Expect = 1.9e-08
Identity = 54/258 (20.93%), Postives = 102/258 (39.53%), Query Frame = 0

Query: 15  FPMLNPPILRRSNLYFGERCIRKFRRSFSVSASVDKVDGGEVRVRFAPSPTGNLHVGGAR 74
           FP      +  + ++F +  I +   +  V     KV GG+V  RF P P G LH+G A+
Sbjct: 230 FPQPEQNFMVHTEVFFSDGSILRCSNTKEVLDKHLKVTGGKVYTRFPPEPNGYLHIGHAK 289

Query: 75  TALFNYLFARANGGKFVLRIEDTDLERSTRQSEEAVLKDLSWLGIDWDEGPNAGGEYGPY 134
               ++  A+  GG   LR +DT+ E    +    + + + W+G  W+          P+
Sbjct: 290 AMFVDFGLAKERGGCCYLRYDDTNPEAEKEEYINHIEEIVKWMG--WE----------PF 349

Query: 135 RQSERNALYKQYAEKLLESGQVYRCFCSNEELERMKEVAKLKQLPPVYMGKWAGATNEEV 194
           + +  +  +++  +  +E  +    +  ++  + +KE  + K   P     W     EE 
Sbjct: 350 KITYTSDYFQELYDLAVELIRRGHAYVDHQTADEIKEYREKKMNSP-----WRDRPIEES 409

Query: 195 --------REELEKGTPYTYRFRVPQEGSLKINDLIRGEVSSPPVLHHPA---------- 253
                   R  +E+G       +  Q  +  + DLI   +   P   HP           
Sbjct: 410 LKLFDEMRRGIIEEGKATLRMKQDMQSDNFNMYDLIAYRIKFAP---HPKAGDKWCIYPS 467