Lsi11G012810 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi11G012810
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	glutelin type-D 1-like
Location	chr11: 21420806 .. 21421972 (-)
RNA-Seq Expression	Lsi11G012810
Synteny	Lsi11G012810

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAATCGATTTGACTCCTCAATTGTCCAAGAAGGTCTACGGTAGTGGTAGTGATGGAGGTTCTTATTATTCTTGGTCTCTCAAAGAGCTTACCATGCTCCGTGAAGGAAACATTGGTGCCTCTAAAGTTGCCCTCAAGAAGAATGGCTTCGCTCTTCCTCACTACTTTGATTCTACCAAGGTTGCTTACGTTCTTCAAGGTAAATTATCAATTTTGCTTACATTCTACTCTATTTGAAAGTTTCAGTTTTAGTAGGAATATGATCTAATTGATGAGTAAAATGTTGTAGGCAATGGAGTAGCTGGAATCATTATGTCGGAATCGGAGGAGAAGATCATTGCAATCAAGAAAGGAGATGCGATTGCTCTCCCATTTGGCATGGTGACATGGTGGTTCAATAAAGAAGACACTGATTTGGTGGTTTTGTTCTTAGGCGACACATCAAAGGCTCATAAATCAGGCGAGTTCACTAACTTCTTCCTAACTGGTGCTAATGGAATCTTCACCGGCTTCTCCACGGAGTTTGTTGAGAGAGCATGGGACATGGATGAGGTGTCGGTGAAATGTATGGTGAAGAACCAAACTGGAACCGGAATTGTAAAATTGAAGGAGGGAACAAAGATGCCAGAGGCAAAGAAGGAGGATCGAAACGGGATGGCAGTGAACTGCGAGGAGGCACCATTGGATGTGGACGTGAAGAACGGGGGACGAGTTGTGGTTTTGAACACGAAGAATTTGCCCTTGGTAGGGGAGGTAGGACTGGGTGCAGATTTGGTTCGATTGGACAGAAATGCGATGTGTTCACCTGGATTCTCGTGTGATTCAGCACTGCAAGTAACGTACATCGTAAAAGGGAGCGGAAGAGCGGAGGTTGTAGGGGTAGACGGGAAGAAGGTTTTAGAAACGAGAGTGAAAGCTGGAGATCTGTTCATAGTACCAAAGTTCTTCGTTGTATCAAAGATCAGAGATCCTGAAGGAATGGAGTGGTTCTCCATTATCACTACTCCCAATCCTGTTTTCACTCACTTGGCTGGCAACATAGGCGTCTGGAAGTCTCTTTCACTAGAAGTTATTCAAGCAACCTTCGATGTGGATATTGATTTGGTGAAGAACTTCTCTTGCAAGAGGGCTTCTGATGCCATCTTCTTCCCTCCTTCCAATTAA

mRNA sequence

ATGGAAATCGATTTGACTCCTCAATTGTCCAAGAAGGTCTACGGTAGTGGTAGTGATGGAGGTTCTTATTATTCTTGGTCTCTCAAAGAGCTTACCATGCTCCGTGAAGGAAACATTGGTGCCTCTAAAGTTGCCCTCAAGAAGAATGGCTTCGCTCTTCCTCACTACTTTGATTCTACCAAGGTTGCTTACGTTCTTCAAGGCAATGGAGTAGCTGGAATCATTATGTCGGAATCGGAGGAGAAGATCATTGCAATCAAGAAAGGAGATGCGATTGCTCTCCCATTTGGCATGGTGACATGGTGGTTCAATAAAGAAGACACTGATTTGGTGGTTTTGTTCTTAGGCGACACATCAAAGGCTCATAAATCAGGCGAGTTCACTAACTTCTTCCTAACTGGTGCTAATGGAATCTTCACCGGCTTCTCCACGGAGTTTGTTGAGAGAGCATGGGACATGGATGAGGTGTCGGTGAAATGTATGGTGAAGAACCAAACTGGAACCGGAATTGTAAAATTGAAGGAGGGAACAAAGATGCCAGAGGCAAAGAAGGAGGATCGAAACGGGATGGCAGTGAACTGCGAGGAGGCACCATTGGATGTGGACGTGAAGAACGGGGGACGAGTTGTGGTTTTGAACACGAAGAATTTGCCCTTGGTAGGGGAGGTAGGACTGGGTGCAGATTTGGTTCGATTGGACAGAAATGCGATGTGTTCACCTGGATTCTCGTGTGATTCAGCACTGCAAGTAACGTACATCGTAAAAGGGAGCGGAAGAGCGGAGGTTGTAGGGGTAGACGGGAAGAAGGTTTTAGAAACGAGAGTGAAAGCTGGAGATCTGTTCATAGTACCAAAGTTCTTCGTTGTATCAAAGATCAGAGATCCTGAAGGAATGGAGTGGTTCTCCATTATCACTACTCCCAATCCTGTTTTCACTCACTTGGCTGGCAACATAGGCGTCTGGAAGTCTCTTTCACTAGAAGTTATTCAAGCAACCTTCGATGTGGATATTGATTTGGTGAAGAACTTCTCTTGCAAGAGGGCTTCTGATGCCATCTTCTTCCCTCCTTCCAATTAA

Coding sequence (CDS)

ATGGAAATCGATTTGACTCCTCAATTGTCCAAGAAGGTCTACGGTAGTGGTAGTGATGGAGGTTCTTATTATTCTTGGTCTCTCAAAGAGCTTACCATGCTCCGTGAAGGAAACATTGGTGCCTCTAAAGTTGCCCTCAAGAAGAATGGCTTCGCTCTTCCTCACTACTTTGATTCTACCAAGGTTGCTTACGTTCTTCAAGGCAATGGAGTAGCTGGAATCATTATGTCGGAATCGGAGGAGAAGATCATTGCAATCAAGAAAGGAGATGCGATTGCTCTCCCATTTGGCATGGTGACATGGTGGTTCAATAAAGAAGACACTGATTTGGTGGTTTTGTTCTTAGGCGACACATCAAAGGCTCATAAATCAGGCGAGTTCACTAACTTCTTCCTAACTGGTGCTAATGGAATCTTCACCGGCTTCTCCACGGAGTTTGTTGAGAGAGCATGGGACATGGATGAGGTGTCGGTGAAATGTATGGTGAAGAACCAAACTGGAACCGGAATTGTAAAATTGAAGGAGGGAACAAAGATGCCAGAGGCAAAGAAGGAGGATCGAAACGGGATGGCAGTGAACTGCGAGGAGGCACCATTGGATGTGGACGTGAAGAACGGGGGACGAGTTGTGGTTTTGAACACGAAGAATTTGCCCTTGGTAGGGGAGGTAGGACTGGGTGCAGATTTGGTTCGATTGGACAGAAATGCGATGTGTTCACCTGGATTCTCGTGTGATTCAGCACTGCAAGTAACGTACATCGTAAAAGGGAGCGGAAGAGCGGAGGTTGTAGGGGTAGACGGGAAGAAGGTTTTAGAAACGAGAGTGAAAGCTGGAGATCTGTTCATAGTACCAAAGTTCTTCGTTGTATCAAAGATCAGAGATCCTGAAGGAATGGAGTGGTTCTCCATTATCACTACTCCCAATCCTGTTTTCACTCACTTGGCTGGCAACATAGGCGTCTGGAAGTCTCTTTCACTAGAAGTTATTCAAGCAACCTTCGATGTGGATATTGATTTGGTGAAGAACTTCTCTTGCAAGAGGGCTTCTGATGCCATCTTCTTCCCTCCTTCCAATTAA

Protein sequence

MEIDLTPQLSKKVYGSGSDGGSYYSWSLKELTMLREGNIGASKVALKKNGFALPHYFDSTKVAYVLQGNGVAGIIMSESEEKIIAIKKGDAIALPFGMVTWWFNKEDTDLVVLFLGDTSKAHKSGEFTNFFLTGANGIFTGFSTEFVERAWDMDEVSVKCMVKNQTGTGIVKLKEGTKMPEAKKEDRNGMAVNCEEAPLDVDVKNGGRVVVLNTKNLPLVGEVGLGADLVRLDRNAMCSPGFSCDSALQVTYIVKGSGRAEVVGVDGKKVLETRVKAGDLFIVPKFFVVSKIRDPEGMEWFSIITTPNPVFTHLAGNIGVWKSLSLEVIQATFDVDIDLVKNFSCKRASDAIFFPPSN

Homology

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BLAST of Lsi11G012810 vs. ExPASy Swiss-Prot
Match: Q9XHP0 (11S globulin seed storage protein 2 OS=Sesamum indicum OX=4182 PE=1 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 2.9e-26
Identity = 89/409 (21.76%), Postives = 178/409 (43.52%), Query Frame = 0


Query: 18  SDGGSYYSWSLKELTMLREGNIGASKVALKKNGFALPHYFDSTKVAYVLQGNGVAGIIM- 77
           S+GG+   W  ++    +   I A +  ++ NG +LP+Y  S ++ Y+ +G G+  I++ 
Sbjct: 50  SEGGTTELWDERQ-EQFQCAGIVAMRSTIRPNGLSLPNYHPSPRLVYIERGQGLISIMVP 109

Query: 78  --------------------SESE---------EKIIAIKKGDAIALPFGMVTWWFNKED 137
                               SE +         +K+  +++GD +A+P G   W +N   
Sbjct: 110 GCAETYQVHRSQRTMERTEASEQQDRGSVRDLHQKVHRLRQGDIVAIPSGAAHWCYNDGS 169

Query: 138 TDLVVLFLGDTSKAHKSGE----FTNFFLTGA---------------NGIFTGFSTEFVE 197
            DLV + + D +  H S +    F  F+L G                + IF  F  E + 
Sbjct: 170 EDLVAVSINDVN--HLSNQLDQKFRAFYLAGGVPRSGEQEQQARQTFHNIFRAFDAELLS 229

Query: 198 RAWDMDEVSVKCMVKNQTGTGIVKL------------KEGTKMPEAKKEDRNGMAVNC-- 257
            A+++ + +++ M   +   G++ +            +EG +    ++ D       C  
Sbjct: 230 EAFNVPQETIRRMQSEEEERGLIVMARERMTFVRPDEEEGEQEHRGRQLDNGLEETFCTM 289

Query: 258 -------EEAPLDVDVKNGGRVVVLNTKNLPLVGEVGLGADLVRLDRNAMCSPGFSCDSA 317
                       D+  +  GRV V++   LP++  + L A+   L  NA+ SP +S  + 
Sbjct: 290 KFRTNVESRREADIFSRQAGRVHVVDRNKLPILKYMDLSAEKGNLYSNALVSPDWSM-TG 349

Query: 318 LQVTYIVKGSGRAEVVGVDGKKVLETRVKAGDLFIVPKFFVVSKIRDPEGMEWFSIITTP 357
             + Y+ +G  + +VV  +G+ ++  RV  G++F+VP+++  +      G EW +  TT 
Sbjct: 350 HTIVYVTRGDAQVQVVDHNGQALMNDRVNQGEMFVVPQYYTSTARAGNNGFEWVAFKTTG 409

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BLAST of Lsi11G012810 vs. ExPASy Swiss-Prot
Match: Q8GZP6 (11S globulin seed storage protein Ana o 2.0101 (Fragment) OS=Anacardium occidentale OX=171929 PE=1 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 1.2e-24
Identity = 93/388 (23.97%), Postives = 163/388 (42.01%), Query Frame = 0


Query: 19  DGGSYYSWSLKELTMLREGNIGASKVALKKNGFALPHYFDSTKVAYVLQGNGVAGII--- 78
           + G+  +W        R   +   +  ++ NG  LP Y ++ ++ YV+QG G+ GI    
Sbjct: 42  EAGTVEAWDPNH-EQFRCAGVALVRHTIQPNGLLLPQYSNAPQLIYVVQGEGMTGISYPG 101

Query: 79  -------------------MSESEEKIIAIKKGDAIALPFGMVTWWFNKEDTDLVVLFLG 138
                                +  +KI   ++GD IA+P G+  W +N+ ++ +V + L 
Sbjct: 102 CPETYQAPQQGRQQGQSGRFQDRHQKIRRFRRGDIIAIPAGVAHWCYNEGNSPVVTVTLL 161

Query: 139 DTSKAHKSGEFT--NFFLTG---------------ANGIFTGFSTEFVERAWDMDEVSVK 198
           D S +    + T   F L G                  +F+GF TE +  A+ +DE  +K
Sbjct: 162 DVSNSQNQLDRTPRKFHLAGNPKDVFQQQQQHQSRGRNLFSGFDTELLAEAFQVDERLIK 221

Query: 199 CMVKNQTGTGIVKLKE---------------GTKMPEAKKEDR-------NGMAVNC--- 258
            +       GIVK+K+               G++  E  ++++       NG+       
Sbjct: 222 QLKSEDNRGGIVKVKDDELRVIRPSRSQSERGSESEEESEDEKRRWGQRDNGIEETICTM 281

Query: 259 -------EEAPLDVDVKNGGRVVVLNTKNLPLVGEVGLGADLVRLDRNAMCSPGFSCDSA 318
                  + A  D+     GR+  LN+ NLP++  + L  +   L +NA+  P ++ +S 
Sbjct: 282 RLKENINDPARADIYTPEVGRLTTLNSLNLPILKWLQLSVEKGVLYKNALVLPHWNLNSH 341

Query: 319 LQVTYIVKGSGRAEVVGVDGKKVLETRVKAGDLFIVPKFFVVSKIRDPEGMEWFSIITTP 336
             + Y  KG G+ +VV   G +V +  V+ G + +VP+ F V K    E  EW S  T  
Sbjct: 342 -SIIYGCKGKGQVQVVDNFGNRVFDGEVREGQMLVVPQNFAVVKRAREERFEWISFKTND 401

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BLAST of Lsi11G012810 vs. ExPASy Swiss-Prot
Match: Q9ZWA9 (12S seed storage protein CRD OS=Arabidopsis thaliana OX=3702 GN=CRD PE=1 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 1.2e-24
Identity = 89/384 (23.18%), Postives = 162/384 (42.19%), Query Frame = 0


Query: 34  LREGNIGASKVALKKNGFALPHYFDSTKVAYVLQGNGVAGIIMS---------------- 93
           LR   +  +++ L+ N   LP +F    +AYV+QG GV G I S                
Sbjct: 67  LRCAGVTVARITLQPNSIFLPAFFSPPALAYVVQGEGVMGTIASGCPETFAEVEGSSGRG 126

Query: 94  ----------ESEEKIIAIKKGDAIALPFGMVTWWFNKEDTDLVVLFLGD-TSKAHKSGE 153
                     +  +K+   ++GD  A   G+  WW+N+ D+D V++ + D T++ ++  +
Sbjct: 127 GGGDPGRRFEDMHQKLENFRRGDVFASLAGVSQWWYNRGDSDAVIVIVLDVTNRENQLDQ 186

Query: 154 FTNFF-LTGA--------------NGIFTGFSTEFVERAWDMDEVSVKCMVKNQTGTGIV 213
               F L G+              N  F+GF    +  A+ ++  + K +   +   G +
Sbjct: 187 VPRMFQLAGSRTQEEEQPLTWPSGNNAFSGFDPNIIAEAFKINIETAKQLQNQKDNRGNI 246

Query: 214 KLKEGT---KMPEAKKEDRNGMAVNCEEAPLDVDV--------------KNGGRVVVLNT 273
               G     +P  ++  ++G+A   EE      +                 GR+  LN+
Sbjct: 247 IRANGPLHFVIPPPREWQQDGIANGIEETYCTAKIHENIDDPERSDHFSTRAGRISTLNS 306

Query: 274 KNLPLVGEVGLGADLVRLDRNAMCSPGFSCDSALQVTYIVKGSGRAEVVGVDGKKVLETR 333
            NLP++  V L A    L    M  P ++  +A  V Y+  G  + +VV  +G+ V   +
Sbjct: 307 LNLPVLRLVRLNALRGYLYSGGMVLPQWTA-NAHTVLYVTGGQAKIQVVDDNGQSVFNEQ 366

Query: 334 VKAGDLFIVPKFFVVSKIRDPEGMEWFSIITTPNPVFTHLAGNIGVWKSLSLEVIQATFD 359
           V  G + ++P+ F VSK     G EW S  T  N     L+G     +++ ++VI+A++ 
Sbjct: 367 VGQGQIIVIPQGFAVSKTAGETGFEWISFKTNDNAYINTLSGQTSYLRAVPVDVIKASYG 426

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BLAST of Lsi11G012810 vs. ExPASy Swiss-Prot
Match: P07730 (Glutelin type-A 2 OS=Oryza sativa subsp. japonica OX=39947 GN=GLUA2 PE=1 SV=1)

HSP 1 Score: 114.8 bits (286), Expect = 2.1e-24
Identity = 92/396 (23.23%), Postives = 168/396 (42.42%), Query Frame = 0


Query: 46  LKKNGFALPHYFDSTKVAYVLQGNGVAG---------------------IIMSESE---- 105
           ++  G  LPHY +   + Y++QG G+ G                     +  S+S+    
Sbjct: 89  IEPRGLLLPHYTNGASLVYIIQGRGITGPTFPGCPETYQQQFQQSGQAQLTESQSQSHKF 148

Query: 106 ----EKIIAIKKGDAIALPFGMVTWWFNKEDTDLVVLFLGDTSKA--HKSGEFTNFFLTG 165
               +KI   ++GD IALP G+  W +N  +  +V +++ D +           +F L G
Sbjct: 149 KDEHQKIHRFRQGDVIALPAGVAHWCYNDGEVPVVAIYVTDINNGANQLDPRQRDFLLAG 208

Query: 166 ---------------ANGIFTGFSTEFVERAWDM-DEVSVKCMVKNQTGTGIVKLKEGTK 225
                          +  IF+GFSTE +  A+ + ++V+ +   +N     IV+++ G  
Sbjct: 209 NKRNPQAYRREVEEWSQNIFSGFSTELLSEAFGISNQVARQLQCQNDQRGEIVRVERGLS 268

Query: 226 MPE---AKKEDRNGMAVNCE---------------------------------EAPLDVD 285
           + +   + +E   G   + E                                 + P   D
Sbjct: 269 LLQPYASLQEQEQGQMQSREHYQEGGYQQSQYGSGCPNGLDETFCTMRVRQNIDNPNRAD 328

Query: 286 VKN--GGRVVVLNTKNLPLVGEVGLGADLVRLDRNAMCSPGFSCDSALQVTYIVKGSGRA 345
             N   GRV  LN++N P++  V + A  V L +NA+ SP ++  +A  + YI +G  + 
Sbjct: 329 TYNPRAGRVTNLNSQNFPILNLVQMSAVKVNLYQNALLSPFWNI-NAHSIVYITQGRAQV 388

Query: 346 EVVGVDGKKVLETRVKAGDLFIVPKFFVVSKIRDPEGMEWFSIITTPNPVFTHLAGNIGV 357
           +VV  +GK V    ++ G L IVP+ +VV K    EG  + +  T PN + +H+AG   +
Sbjct: 389 QVVNNNGKTVFNGELRRGQLLIVPQHYVVVKKAQREGCAYIAFKTNPNSMVSHIAGKSSI 448

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BLAST of Lsi11G012810 vs. ExPASy Swiss-Prot
Match: P14614 (Glutelin type-B 4 OS=Oryza sativa subsp. japonica OX=39947 GN=GLUB4 PE=1 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 2.7e-24
Identity = 94/394 (23.86%), Postives = 171/394 (43.40%), Query Frame = 0


Query: 46  LKKNGFALPHYFDSTKVAYVLQGNGVAGII-------------------------MSESE 105
           ++  G  +P Y ++  + Y++QG G  G+                            +  
Sbjct: 88  IEPQGLLVPRYSNTPGMVYIIQGRGSMGLTFPGCPATYQQQFQQFLPEGQSQSQKFRDEH 147

Query: 106 EKIIAIKKGDAIALPFGMVTWWFNKEDTDLVVLFLGDTSKAHKSGE--FTNFFLTGAN-- 165
           +KI   ++GD +ALP G+  W++N+ D  +V L++ D +      E     F L G N  
Sbjct: 148 QKIHQFRQGDIVALPAGVAHWFYNEGDAPVVALYVFDLNNNANQLEPRQKEFLLAGNNNR 207

Query: 166 ---------------GIFTGFSTEFVERAWDMDE-VSVKCMVKNQTGTGIVKLKEGTKM- 225
                           IF+GF+ E +  A  ++  V+ +   +N     I+++K G K+ 
Sbjct: 208 EQQMYGRSIEQHSGQNIFSGFNNELLSEALGVNALVAKRLQGQNDQRGEIIRVKNGLKLL 267

Query: 226 --------PEAKKEDR-------------------NGMAVN-CE-------EAPLDVDVK 285
                    +A+++++                   NG+  N C        E P   D  
Sbjct: 268 RPAFAQQQEQAQQQEQAQAQYQVQYSEEQQPSTRCNGLDENFCTIKARLNIENPSHADTY 327

Query: 286 N--GGRVVVLNTKNLPLVGEVGLGADLVRLDRNAMCSPGFSCDSALQVTYIVKGSGRAEV 345
           N   GR+  LN++  P++  V L A  V L +NA+ SP ++  +A  + YIV+G  R +V
Sbjct: 328 NPRAGRITRLNSQKFPILNLVQLSATRVNLYQNAILSPFWNV-NAHSLVYIVQGHARVQV 387

Query: 346 VGVDGKKVLETRVKAGDLFIVPKFFVVSKIRDPEGMEWFSIITTPNPVFTHLAGNIGVWK 357
           V   GK V    ++ G L I+P+ +VV K  + EG ++ S  T  N + +HLAG   +++
Sbjct: 388 VSNLGKTVFNGVLRPGQLLIIPQHYVVLKKAEHEGCQYISFKTNANSMVSHLAGKNSIFR 447

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BLAST of Lsi11G012810 vs. ExPASy TrEMBL
Match: A0A0A0K666 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G281380 PE=3 SV=1)

HSP 1 Score: 638.3 bits (1645), Expect = 2.0e-179
Identity = 317/358 (88.55%), Postives = 337/358 (94.13%), Query Frame = 0


Query: 1   MEIDLTPQLSKKVYGSGSDGGSYYSWSLKELTMLREGNIGASKVALKKNGFALPHYFDST 60
           MEIDLTPQL KK+Y  GSDGGSYY+WS KEL MLREGNIGASK+AL+KNGFALP Y DS 
Sbjct: 1   MEIDLTPQLPKKIY--GSDGGSYYAWSPKELPMLREGNIGASKLALEKNGFALPRYSDSA 60

Query: 61  KVAYVLQGNGVAGIIMSESEEKIIAIKKGDAIALPFGMVTWWFNKEDTDLVVLFLGDTSK 120
           KVAYVLQGNGVAGII+ ESEEK+IAIKKGDAIALPFG+VTWWFNKE TDLVVLFLGDTSK
Sbjct: 61  KVAYVLQGNGVAGIILPESEEKVIAIKKGDAIALPFGVVTWWFNKEATDLVVLFLGDTSK 120

Query: 121 AHKSGEFTNFFLTGANGIFTGFSTEFVERAWDMDEVSVKCMVKNQTGTGIVKLKEGTKMP 180
           AHKSGEFT+FFLTGANGIFTGFSTEFV RAWDMDE SVK +VKNQTGTGIVKLKEGTKMP
Sbjct: 121 AHKSGEFTDFFLTGANGIFTGFSTEFVGRAWDMDEASVKSLVKNQTGTGIVKLKEGTKMP 180

Query: 181 EAKKEDRNGMAVNCEEAPLDVDVKNGGRVVVLNTKNLPLVGEVGLGADLVRLDRNAMCSP 240
           E KKE RNGMA+NCEEAPLDVDVKNGGRVVVLNTKNLPLVGEVGLGADLVRLD +AMCSP
Sbjct: 181 EPKKEHRNGMALNCEEAPLDVDVKNGGRVVVLNTKNLPLVGEVGLGADLVRLDGSAMCSP 240

Query: 241 GFSCDSALQVTYIVKGSGRAEVVGVDGKKVLETRVKAGDLFIVPKFFVVSKIRDPEGMEW 300
           GFSCDSALQVTYIVKGSGRAEVVGVDGKKVLETRVKAG+LFIVP+FFVVSKI DPEGMEW
Sbjct: 241 GFSCDSALQVTYIVKGSGRAEVVGVDGKKVLETRVKAGNLFIVPRFFVVSKIGDPEGMEW 300

Query: 301 FSIITTPNPVFTHLAGNIGVWKSLSLEVIQATFDVDIDLVKNFSCKRASDAIFFPPSN 359
           FSII+TPNPVFTHLAG+IGVWK+LS EVI+A F+V+ DLVKNFS KR+SDAIFFPPSN
Sbjct: 301 FSIISTPNPVFTHLAGSIGVWKALSPEVIEAAFNVEADLVKNFSSKRSSDAIFFPPSN 356

[Close]

BLAST of Lsi11G012810 vs. ExPASy TrEMBL
Match: A0A5A7UAB0 (Glutelin type-B 5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold675G00320 PE=3 SV=1)

HSP 1 Score: 637.1 bits (1642), Expect = 4.4e-179
Identity = 317/358 (88.55%), Postives = 336/358 (93.85%), Query Frame = 0


Query: 1   MEIDLTPQLSKKVYGSGSDGGSYYSWSLKELTMLREGNIGASKVALKKNGFALPHYFDST 60
           MEIDLTPQL KK+Y  G DGGSYYSWS KEL MLREGNIGASK+AL+KNGFALP Y DS 
Sbjct: 1   MEIDLTPQLPKKIY--GGDGGSYYSWSPKELPMLREGNIGASKLALEKNGFALPRYSDSA 60

Query: 61  KVAYVLQGNGVAGIIMSESEEKIIAIKKGDAIALPFGMVTWWFNKEDTDLVVLFLGDTSK 120
           KVAYVLQG+GVAGII+ ESEEK+IAIKKGDAIALPFG+VTWWFNKE TDLVVLFLGDTSK
Sbjct: 61  KVAYVLQGSGVAGIILPESEEKVIAIKKGDAIALPFGVVTWWFNKEATDLVVLFLGDTSK 120

Query: 121 AHKSGEFTNFFLTGANGIFTGFSTEFVERAWDMDEVSVKCMVKNQTGTGIVKLKEGTKMP 180
           AHKSGEFT+FFLTGANGIFTGFSTEFV RAWDMDE SVK +VKNQTGTGIVKLKEGTKMP
Sbjct: 121 AHKSGEFTDFFLTGANGIFTGFSTEFVGRAWDMDEASVKSLVKNQTGTGIVKLKEGTKMP 180

Query: 181 EAKKEDRNGMAVNCEEAPLDVDVKNGGRVVVLNTKNLPLVGEVGLGADLVRLDRNAMCSP 240
           E KKE RNGMA+NCEEAPLDVDVKNGGRVVVLNTKNLPLVGEVGLGADLVRLD +AMCSP
Sbjct: 181 EPKKEHRNGMALNCEEAPLDVDVKNGGRVVVLNTKNLPLVGEVGLGADLVRLDGSAMCSP 240

Query: 241 GFSCDSALQVTYIVKGSGRAEVVGVDGKKVLETRVKAGDLFIVPKFFVVSKIRDPEGMEW 300
           GFSCDSALQVTYIVKGSGRAEVVGVDGKKVLETRVKAG+LFIVP+FFVVSKI DPEGMEW
Sbjct: 241 GFSCDSALQVTYIVKGSGRAEVVGVDGKKVLETRVKAGNLFIVPRFFVVSKIGDPEGMEW 300

Query: 301 FSIITTPNPVFTHLAGNIGVWKSLSLEVIQATFDVDIDLVKNFSCKRASDAIFFPPSN 359
           FSII+TPNPVFTHLAG+IGVWK+LS EVIQA F+V+ DLVKNFS KR+SDAIFFPPSN
Sbjct: 301 FSIISTPNPVFTHLAGSIGVWKALSPEVIQAAFNVEADLVKNFSSKRSSDAIFFPPSN 356

[Close]

BLAST of Lsi11G012810 vs. ExPASy TrEMBL
Match: A0A1S3CG59 (glutelin type-B 5-like OS=Cucumis melo OX=3656 GN=LOC103500083 PE=3 SV=1)

HSP 1 Score: 637.1 bits (1642), Expect = 4.4e-179
Identity = 317/358 (88.55%), Postives = 336/358 (93.85%), Query Frame = 0


Query: 1   MEIDLTPQLSKKVYGSGSDGGSYYSWSLKELTMLREGNIGASKVALKKNGFALPHYFDST 60
           MEIDLTPQL KK+Y  G DGGSYYSWS KEL MLREGNIGASK+AL+KNGFALP Y DS 
Sbjct: 1   MEIDLTPQLPKKIY--GGDGGSYYSWSPKELPMLREGNIGASKLALEKNGFALPRYSDSA 60

Query: 61  KVAYVLQGNGVAGIIMSESEEKIIAIKKGDAIALPFGMVTWWFNKEDTDLVVLFLGDTSK 120
           KVAYVLQG+GVAGII+ ESEEK+IAIKKGDAIALPFG+VTWWFNKE TDLVVLFLGDTSK
Sbjct: 61  KVAYVLQGSGVAGIILPESEEKVIAIKKGDAIALPFGVVTWWFNKEATDLVVLFLGDTSK 120

Query: 121 AHKSGEFTNFFLTGANGIFTGFSTEFVERAWDMDEVSVKCMVKNQTGTGIVKLKEGTKMP 180
           AHKSGEFT+FFLTGANGIFTGFSTEFV RAWDMDE SVK +VKNQTGTGIVKLKEGTKMP
Sbjct: 121 AHKSGEFTDFFLTGANGIFTGFSTEFVGRAWDMDEASVKSLVKNQTGTGIVKLKEGTKMP 180

Query: 181 EAKKEDRNGMAVNCEEAPLDVDVKNGGRVVVLNTKNLPLVGEVGLGADLVRLDRNAMCSP 240
           E KKE RNGMA+NCEEAPLDVDVKNGGRVVVLNTKNLPLVGEVGLGADLVRLD +AMCSP
Sbjct: 181 EPKKEHRNGMALNCEEAPLDVDVKNGGRVVVLNTKNLPLVGEVGLGADLVRLDGSAMCSP 240

Query: 241 GFSCDSALQVTYIVKGSGRAEVVGVDGKKVLETRVKAGDLFIVPKFFVVSKIRDPEGMEW 300
           GFSCDSALQVTYIVKGSGRAEVVGVDGKKVLETRVKAG+LFIVP+FFVVSKI DPEGMEW
Sbjct: 241 GFSCDSALQVTYIVKGSGRAEVVGVDGKKVLETRVKAGNLFIVPRFFVVSKIGDPEGMEW 300

Query: 301 FSIITTPNPVFTHLAGNIGVWKSLSLEVIQATFDVDIDLVKNFSCKRASDAIFFPPSN 359
           FSII+TPNPVFTHLAG+IGVWK+LS EVIQA F+V+ DLVKNFS KR+SDAIFFPPSN
Sbjct: 301 FSIISTPNPVFTHLAGSIGVWKALSPEVIQAAFNVEADLVKNFSSKRSSDAIFFPPSN 356

[Close]

BLAST of Lsi11G012810 vs. ExPASy TrEMBL
Match: A0A6J1EX25 (glutelin type-D 1-like OS=Cucurbita moschata OX=3662 GN=LOC111439037 PE=3 SV=1)

HSP 1 Score: 634.4 bits (1635), Expect = 2.8e-178
Identity = 316/357 (88.52%), Postives = 335/357 (93.84%), Query Frame = 0


Query: 1   MEIDLTPQLSKKVYGSGSDGGSYYSWSLKELTMLREGNIGASKVALKKNGFALPHYFDST 60
           ME+DLTPQL+KK+Y   SDGGSYYSWS KEL MLREGNIGA+K+AL+KNGFALP Y DS 
Sbjct: 1   MEMDLTPQLAKKIY--VSDGGSYYSWSPKELPMLREGNIGAAKLALEKNGFALPRYSDSA 60

Query: 61  KVAYVLQGNGVAGIIMSESEEKIIAIKKGDAIALPFGMVTWWFNKEDTDLVVLFLGDTSK 120
           KVAYVLQGNGVAGII+ ESEEK+IAIKKGDAIALPFG+VTWWFNKE TDLVVLFLGDTSK
Sbjct: 61  KVAYVLQGNGVAGIILPESEEKVIAIKKGDAIALPFGVVTWWFNKEATDLVVLFLGDTSK 120

Query: 121 AHKSGEFTNFFLTGANGIFTGFSTEFVERAWDMDEVSVKCMVKNQTGTGIVKLKEGTKMP 180
           AHKSGEFT+FFLTGANGIFTGFSTEFV RAWDMDE SVK +VKNQTGTGIVKLK+G KMP
Sbjct: 121 AHKSGEFTDFFLTGANGIFTGFSTEFVGRAWDMDEASVKSLVKNQTGTGIVKLKDGVKMP 180

Query: 181 EAKKEDRNGMAVNCEEAPLDVDVKNGGRVVVLNTKNLPLVGEVGLGADLVRLDRNAMCSP 240
           E KKE RNGMA+NCEEAPLDVDVKNGGRVVVLNTKNLPLVGEVGLGADLVRLD +AMCSP
Sbjct: 181 EPKKEHRNGMALNCEEAPLDVDVKNGGRVVVLNTKNLPLVGEVGLGADLVRLDGSAMCSP 240

Query: 241 GFSCDSALQVTYIVKGSGRAEVVGVDGKKVLETRVKAGDLFIVPKFFVVSKIRDPEGMEW 300
           GFSCDSALQVTYIV+GSGRAEVVGVDGKKVLETRVKAG+LFIVP+FFVVSKI DPEGMEW
Sbjct: 241 GFSCDSALQVTYIVRGSGRAEVVGVDGKKVLETRVKAGNLFIVPRFFVVSKIGDPEGMEW 300

Query: 301 FSIITTPNPVFTHLAGNIGVWKSLSLEVIQATFDVDIDLVKNFSCKRASDAIFFPPS 358
           FSIITTPNPVFTHLAG+IGVWKSLS EVIQA F+VD DLVKNFS KRASDAIFFPPS
Sbjct: 301 FSIITTPNPVFTHLAGSIGVWKSLSPEVIQAAFNVDADLVKNFSSKRASDAIFFPPS 355

[Close]

BLAST of Lsi11G012810 vs. ExPASy TrEMBL
Match: A0A6J1IH21 (glutelin type-D 1-like OS=Cucurbita maxima OX=3661 GN=LOC111477153 PE=3 SV=1)

HSP 1 Score: 633.6 bits (1633), Expect = 4.9e-178
Identity = 315/357 (88.24%), Postives = 335/357 (93.84%), Query Frame = 0


Query: 1   MEIDLTPQLSKKVYGSGSDGGSYYSWSLKELTMLREGNIGASKVALKKNGFALPHYFDST 60
           MEIDLTPQL+KK+Y  G DGGSYYSWS KEL MLREGNIGA+K+AL+KNGFALP Y DS 
Sbjct: 1   MEIDLTPQLAKKIY--GCDGGSYYSWSPKELPMLREGNIGAAKLALEKNGFALPRYSDSA 60

Query: 61  KVAYVLQGNGVAGIIMSESEEKIIAIKKGDAIALPFGMVTWWFNKEDTDLVVLFLGDTSK 120
           KVAYVLQGNGVAGII+ ESEEK+IAIKKGDAIALPFG+VTWWFNKE TDLVVLFLGDTSK
Sbjct: 61  KVAYVLQGNGVAGIILPESEEKVIAIKKGDAIALPFGVVTWWFNKEATDLVVLFLGDTSK 120

Query: 121 AHKSGEFTNFFLTGANGIFTGFSTEFVERAWDMDEVSVKCMVKNQTGTGIVKLKEGTKMP 180
           AHKSGEFT+FFLTGANGIFTGFSTEFV RAWDMDE SVK +VK+QTGTGIVKLK+G KMP
Sbjct: 121 AHKSGEFTDFFLTGANGIFTGFSTEFVGRAWDMDEASVKSLVKSQTGTGIVKLKDGVKMP 180

Query: 181 EAKKEDRNGMAVNCEEAPLDVDVKNGGRVVVLNTKNLPLVGEVGLGADLVRLDRNAMCSP 240
           E KKE RNGMA+NCEEAPLDVDVKNGGRVVVLNTKNLPLVGEVGLGADLVRLD +AMCSP
Sbjct: 181 EPKKEHRNGMALNCEEAPLDVDVKNGGRVVVLNTKNLPLVGEVGLGADLVRLDGSAMCSP 240

Query: 241 GFSCDSALQVTYIVKGSGRAEVVGVDGKKVLETRVKAGDLFIVPKFFVVSKIRDPEGMEW 300
           GFSCDSALQVTYIV+GSGRAEVVGVDGKKVLETRVKAG+LFIVP+FFVVSKI DPEGMEW
Sbjct: 241 GFSCDSALQVTYIVRGSGRAEVVGVDGKKVLETRVKAGNLFIVPRFFVVSKIGDPEGMEW 300

Query: 301 FSIITTPNPVFTHLAGNIGVWKSLSLEVIQATFDVDIDLVKNFSCKRASDAIFFPPS 358
           FSII+TPNPVFTHLAG+IGVWKSLS EVIQA F+VD DLVKNFS KRASDAIFFPPS
Sbjct: 301 FSIISTPNPVFTHLAGSIGVWKSLSPEVIQAAFNVDADLVKNFSSKRASDAIFFPPS 355

[Close]

BLAST of Lsi11G012810 vs. NCBI nr
Match: KAG6592225.1 (Glutelin type-D 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7025082.1 Glutelin type-B 5 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 639.0 bits (1647), Expect = 2.4e-179
Identity = 318/357 (89.08%), Postives = 336/357 (94.12%), Query Frame = 0


Query: 1   MEIDLTPQLSKKVYGSGSDGGSYYSWSLKELTMLREGNIGASKVALKKNGFALPHYFDST 60
           MEIDLTPQL+KK+Y  GSDGGSYYSWS KEL MLREGNIGA+K+AL+KNGFALP Y DS 
Sbjct: 1   MEIDLTPQLAKKIY--GSDGGSYYSWSPKELPMLREGNIGAAKLALEKNGFALPRYSDSA 60

Query: 61  KVAYVLQGNGVAGIIMSESEEKIIAIKKGDAIALPFGMVTWWFNKEDTDLVVLFLGDTSK 120
           KVAYVLQGNGVAGII+ ESEEK+IAIKKGDAIALPFG+VTWWFNKE TDLVVLFLGDTSK
Sbjct: 61  KVAYVLQGNGVAGIILPESEEKVIAIKKGDAIALPFGVVTWWFNKEATDLVVLFLGDTSK 120

Query: 121 AHKSGEFTNFFLTGANGIFTGFSTEFVERAWDMDEVSVKCMVKNQTGTGIVKLKEGTKMP 180
           AHKSGEFT+FFLTGANGIFTGFSTEFV RAWDMDE SVK +VKNQTGTGIVKLK+G KMP
Sbjct: 121 AHKSGEFTDFFLTGANGIFTGFSTEFVGRAWDMDEASVKSLVKNQTGTGIVKLKDGVKMP 180

Query: 181 EAKKEDRNGMAVNCEEAPLDVDVKNGGRVVVLNTKNLPLVGEVGLGADLVRLDRNAMCSP 240
           E KKE RNGMA+NCEEAPLDVDVKNGGRVVVLNTKNLPLVGEVGLGADLVRLD +AMCSP
Sbjct: 181 EPKKEHRNGMALNCEEAPLDVDVKNGGRVVVLNTKNLPLVGEVGLGADLVRLDGSAMCSP 240

Query: 241 GFSCDSALQVTYIVKGSGRAEVVGVDGKKVLETRVKAGDLFIVPKFFVVSKIRDPEGMEW 300
           GFSCDSALQVTYIV+GSGRAEVVGVDGKKVLETRVKAG+LFIVP+FFVVSKI DPEGMEW
Sbjct: 241 GFSCDSALQVTYIVRGSGRAEVVGVDGKKVLETRVKAGNLFIVPRFFVVSKIGDPEGMEW 300

Query: 301 FSIITTPNPVFTHLAGNIGVWKSLSLEVIQATFDVDIDLVKNFSCKRASDAIFFPPS 358
           FSIITTPNPVFTHLAG+IGVWKSLS EVIQA F+VD DLVKNFS KRASDAIFFPPS
Sbjct: 301 FSIITTPNPVFTHLAGSIGVWKSLSPEVIQAAFNVDADLVKNFSSKRASDAIFFPPS 355

[Close]

BLAST of Lsi11G012810 vs. NCBI nr
Match: XP_004150394.1 (glutelin type-D 1 [Cucumis sativus] >KGN44409.1 hypothetical protein Csa_015780 [Cucumis sativus])

HSP 1 Score: 638.3 bits (1645), Expect = 4.1e-179
Identity = 317/358 (88.55%), Postives = 337/358 (94.13%), Query Frame = 0


Query: 1   MEIDLTPQLSKKVYGSGSDGGSYYSWSLKELTMLREGNIGASKVALKKNGFALPHYFDST 60
           MEIDLTPQL KK+Y  GSDGGSYY+WS KEL MLREGNIGASK+AL+KNGFALP Y DS 
Sbjct: 1   MEIDLTPQLPKKIY--GSDGGSYYAWSPKELPMLREGNIGASKLALEKNGFALPRYSDSA 60

Query: 61  KVAYVLQGNGVAGIIMSESEEKIIAIKKGDAIALPFGMVTWWFNKEDTDLVVLFLGDTSK 120
           KVAYVLQGNGVAGII+ ESEEK+IAIKKGDAIALPFG+VTWWFNKE TDLVVLFLGDTSK
Sbjct: 61  KVAYVLQGNGVAGIILPESEEKVIAIKKGDAIALPFGVVTWWFNKEATDLVVLFLGDTSK 120

Query: 121 AHKSGEFTNFFLTGANGIFTGFSTEFVERAWDMDEVSVKCMVKNQTGTGIVKLKEGTKMP 180
           AHKSGEFT+FFLTGANGIFTGFSTEFV RAWDMDE SVK +VKNQTGTGIVKLKEGTKMP
Sbjct: 121 AHKSGEFTDFFLTGANGIFTGFSTEFVGRAWDMDEASVKSLVKNQTGTGIVKLKEGTKMP 180

Query: 181 EAKKEDRNGMAVNCEEAPLDVDVKNGGRVVVLNTKNLPLVGEVGLGADLVRLDRNAMCSP 240
           E KKE RNGMA+NCEEAPLDVDVKNGGRVVVLNTKNLPLVGEVGLGADLVRLD +AMCSP
Sbjct: 181 EPKKEHRNGMALNCEEAPLDVDVKNGGRVVVLNTKNLPLVGEVGLGADLVRLDGSAMCSP 240

Query: 241 GFSCDSALQVTYIVKGSGRAEVVGVDGKKVLETRVKAGDLFIVPKFFVVSKIRDPEGMEW 300
           GFSCDSALQVTYIVKGSGRAEVVGVDGKKVLETRVKAG+LFIVP+FFVVSKI DPEGMEW
Sbjct: 241 GFSCDSALQVTYIVKGSGRAEVVGVDGKKVLETRVKAGNLFIVPRFFVVSKIGDPEGMEW 300

Query: 301 FSIITTPNPVFTHLAGNIGVWKSLSLEVIQATFDVDIDLVKNFSCKRASDAIFFPPSN 359
           FSII+TPNPVFTHLAG+IGVWK+LS EVI+A F+V+ DLVKNFS KR+SDAIFFPPSN
Sbjct: 301 FSIISTPNPVFTHLAGSIGVWKALSPEVIEAAFNVEADLVKNFSSKRSSDAIFFPPSN 356

[Close]

BLAST of Lsi11G012810 vs. NCBI nr
Match: XP_023535755.1 (glutelin type-D 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 637.5 bits (1643), Expect = 6.9e-179
Identity = 317/357 (88.80%), Postives = 336/357 (94.12%), Query Frame = 0


Query: 1   MEIDLTPQLSKKVYGSGSDGGSYYSWSLKELTMLREGNIGASKVALKKNGFALPHYFDST 60
           MEIDLTPQL+KK+Y  GSDGGSYYSWS KEL MLREGNIGA+K+AL+KNGFALP Y DS 
Sbjct: 1   MEIDLTPQLAKKIY--GSDGGSYYSWSPKELPMLREGNIGAAKLALEKNGFALPRYSDSA 60

Query: 61  KVAYVLQGNGVAGIIMSESEEKIIAIKKGDAIALPFGMVTWWFNKEDTDLVVLFLGDTSK 120
           KVAYVLQGNGVAGII+ ESEEK+IAIKKGDAIALPFG+VTWWFNKE TDLVVLFLGDTSK
Sbjct: 61  KVAYVLQGNGVAGIILPESEEKVIAIKKGDAIALPFGVVTWWFNKEATDLVVLFLGDTSK 120

Query: 121 AHKSGEFTNFFLTGANGIFTGFSTEFVERAWDMDEVSVKCMVKNQTGTGIVKLKEGTKMP 180
           AHKSGEFT+FFLTGANGIFTGFSTEFV RAWDMDE SVK +VKNQTGTGIVKLK+G KMP
Sbjct: 121 AHKSGEFTDFFLTGANGIFTGFSTEFVGRAWDMDEASVKSLVKNQTGTGIVKLKDGVKMP 180

Query: 181 EAKKEDRNGMAVNCEEAPLDVDVKNGGRVVVLNTKNLPLVGEVGLGADLVRLDRNAMCSP 240
           E KKE RNGMA+NCEEAPLDVDVKNGGRVVVLNTKNLPLVGEVGLGADLVRLD +AMCSP
Sbjct: 181 EPKKEHRNGMALNCEEAPLDVDVKNGGRVVVLNTKNLPLVGEVGLGADLVRLDGSAMCSP 240

Query: 241 GFSCDSALQVTYIVKGSGRAEVVGVDGKKVLETRVKAGDLFIVPKFFVVSKIRDPEGMEW 300
           GFSCDSALQVTYIV+GSGRAEVVGVDGKKVLETRVKAG+LFIVP+FFVVSKI DPEGMEW
Sbjct: 241 GFSCDSALQVTYIVRGSGRAEVVGVDGKKVLETRVKAGNLFIVPRFFVVSKIGDPEGMEW 300

Query: 301 FSIITTPNPVFTHLAGNIGVWKSLSLEVIQATFDVDIDLVKNFSCKRASDAIFFPPS 358
           FSII+TPNPVFTHLAG+IGVWKSLS EVIQA F+VD DLVKNFS KRASDAIFFPPS
Sbjct: 301 FSIISTPNPVFTHLAGSIGVWKSLSPEVIQAAFNVDADLVKNFSSKRASDAIFFPPS 355

[Close]

BLAST of Lsi11G012810 vs. NCBI nr
Match: XP_008461502.1 (PREDICTED: glutelin type-B 5-like [Cucumis melo] >KAA0052863.1 glutelin type-B 5-like [Cucumis melo var. makuwa] >TYK04362.1 glutelin type-B 5-like [Cucumis melo var. makuwa])

HSP 1 Score: 637.1 bits (1642), Expect = 9.1e-179
Identity = 317/358 (88.55%), Postives = 336/358 (93.85%), Query Frame = 0


Query: 1   MEIDLTPQLSKKVYGSGSDGGSYYSWSLKELTMLREGNIGASKVALKKNGFALPHYFDST 60
           MEIDLTPQL KK+Y  G DGGSYYSWS KEL MLREGNIGASK+AL+KNGFALP Y DS 
Sbjct: 1   MEIDLTPQLPKKIY--GGDGGSYYSWSPKELPMLREGNIGASKLALEKNGFALPRYSDSA 60

Query: 61  KVAYVLQGNGVAGIIMSESEEKIIAIKKGDAIALPFGMVTWWFNKEDTDLVVLFLGDTSK 120
           KVAYVLQG+GVAGII+ ESEEK+IAIKKGDAIALPFG+VTWWFNKE TDLVVLFLGDTSK
Sbjct: 61  KVAYVLQGSGVAGIILPESEEKVIAIKKGDAIALPFGVVTWWFNKEATDLVVLFLGDTSK 120

Query: 121 AHKSGEFTNFFLTGANGIFTGFSTEFVERAWDMDEVSVKCMVKNQTGTGIVKLKEGTKMP 180
           AHKSGEFT+FFLTGANGIFTGFSTEFV RAWDMDE SVK +VKNQTGTGIVKLKEGTKMP
Sbjct: 121 AHKSGEFTDFFLTGANGIFTGFSTEFVGRAWDMDEASVKSLVKNQTGTGIVKLKEGTKMP 180

Query: 181 EAKKEDRNGMAVNCEEAPLDVDVKNGGRVVVLNTKNLPLVGEVGLGADLVRLDRNAMCSP 240
           E KKE RNGMA+NCEEAPLDVDVKNGGRVVVLNTKNLPLVGEVGLGADLVRLD +AMCSP
Sbjct: 181 EPKKEHRNGMALNCEEAPLDVDVKNGGRVVVLNTKNLPLVGEVGLGADLVRLDGSAMCSP 240

Query: 241 GFSCDSALQVTYIVKGSGRAEVVGVDGKKVLETRVKAGDLFIVPKFFVVSKIRDPEGMEW 300
           GFSCDSALQVTYIVKGSGRAEVVGVDGKKVLETRVKAG+LFIVP+FFVVSKI DPEGMEW
Sbjct: 241 GFSCDSALQVTYIVKGSGRAEVVGVDGKKVLETRVKAGNLFIVPRFFVVSKIGDPEGMEW 300

Query: 301 FSIITTPNPVFTHLAGNIGVWKSLSLEVIQATFDVDIDLVKNFSCKRASDAIFFPPSN 359
           FSII+TPNPVFTHLAG+IGVWK+LS EVIQA F+V+ DLVKNFS KR+SDAIFFPPSN
Sbjct: 301 FSIISTPNPVFTHLAGSIGVWKALSPEVIQAAFNVEADLVKNFSSKRSSDAIFFPPSN 356

[Close]

BLAST of Lsi11G012810 vs. NCBI nr
Match: XP_022932542.1 (glutelin type-D 1-like [Cucurbita moschata])

HSP 1 Score: 634.4 bits (1635), Expect = 5.9e-178
Identity = 316/357 (88.52%), Postives = 335/357 (93.84%), Query Frame = 0


Query: 1   MEIDLTPQLSKKVYGSGSDGGSYYSWSLKELTMLREGNIGASKVALKKNGFALPHYFDST 60
           ME+DLTPQL+KK+Y   SDGGSYYSWS KEL MLREGNIGA+K+AL+KNGFALP Y DS 
Sbjct: 1   MEMDLTPQLAKKIY--VSDGGSYYSWSPKELPMLREGNIGAAKLALEKNGFALPRYSDSA 60

Query: 61  KVAYVLQGNGVAGIIMSESEEKIIAIKKGDAIALPFGMVTWWFNKEDTDLVVLFLGDTSK 120
           KVAYVLQGNGVAGII+ ESEEK+IAIKKGDAIALPFG+VTWWFNKE TDLVVLFLGDTSK
Sbjct: 61  KVAYVLQGNGVAGIILPESEEKVIAIKKGDAIALPFGVVTWWFNKEATDLVVLFLGDTSK 120

Query: 121 AHKSGEFTNFFLTGANGIFTGFSTEFVERAWDMDEVSVKCMVKNQTGTGIVKLKEGTKMP 180
           AHKSGEFT+FFLTGANGIFTGFSTEFV RAWDMDE SVK +VKNQTGTGIVKLK+G KMP
Sbjct: 121 AHKSGEFTDFFLTGANGIFTGFSTEFVGRAWDMDEASVKSLVKNQTGTGIVKLKDGVKMP 180

Query: 181 EAKKEDRNGMAVNCEEAPLDVDVKNGGRVVVLNTKNLPLVGEVGLGADLVRLDRNAMCSP 240
           E KKE RNGMA+NCEEAPLDVDVKNGGRVVVLNTKNLPLVGEVGLGADLVRLD +AMCSP
Sbjct: 181 EPKKEHRNGMALNCEEAPLDVDVKNGGRVVVLNTKNLPLVGEVGLGADLVRLDGSAMCSP 240

Query: 241 GFSCDSALQVTYIVKGSGRAEVVGVDGKKVLETRVKAGDLFIVPKFFVVSKIRDPEGMEW 300
           GFSCDSALQVTYIV+GSGRAEVVGVDGKKVLETRVKAG+LFIVP+FFVVSKI DPEGMEW
Sbjct: 241 GFSCDSALQVTYIVRGSGRAEVVGVDGKKVLETRVKAGNLFIVPRFFVVSKIGDPEGMEW 300

Query: 301 FSIITTPNPVFTHLAGNIGVWKSLSLEVIQATFDVDIDLVKNFSCKRASDAIFFPPS 358
           FSIITTPNPVFTHLAG+IGVWKSLS EVIQA F+VD DLVKNFS KRASDAIFFPPS
Sbjct: 301 FSIITTPNPVFTHLAGSIGVWKSLSPEVIQAAFNVDADLVKNFSSKRASDAIFFPPS 355

[Close]

BLAST of Lsi11G012810 vs. TAIR 10
Match: AT2G28680.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 531.2 bits (1367), Expect = 6.5e-151
Identity = 256/358 (71.51%), Postives = 301/358 (84.08%), Query Frame = 0


Query: 1   MEIDLTPQLSKKVYGSGSDGGSYYSWSLKELTMLREGNIGASKVALKKNGFALPHYFDST 60
           ME+DL+P+L KKVY  G DGGSY++W  +EL MLR+GNIGASK+AL+K G ALP Y DS 
Sbjct: 1   MELDLSPRLPKKVY--GGDGGSYFAWCPEELPMLRDGNIGASKLALEKYGLALPRYSDSP 60

Query: 61  KVAYVLQGNGVAGIIMSESEEKIIAIKKGDAIALPFGMVTWWFNKEDTDLVVLFLGDTSK 120
           KVAYVLQG G AGI++ E EEK+IAIKKGD+IALPFG+VTWWFN EDT+LVVLFLG+T K
Sbjct: 61  KVAYVLQGAGTAGIVLPEKEEKVIAIKKGDSIALPFGVVTWWFNNEDTELVVLFLGETHK 120

Query: 121 AHKSGEFTNFFLTGANGIFTGFSTEFVERAWDMDEVSVKCMVKNQTGTGIVKLKEGTKMP 180
            HK+G+FT+F+LTG+NGIFTGFSTEFV RAWD+DE +VK +V +QTG GIVK+    KMP
Sbjct: 121 GHKAGQFTDFYLTGSNGIFTGFSTEFVGRAWDLDETTVKKLVGSQTGNGIVKVDASLKMP 180

Query: 181 EAKKEDRNGMAVNCEEAPLDVDVKNGGRVVVLNTKNLPLVGEVGLGADLVRLDRNAMCSP 240
           E KK DR G  +NC EAPLDVD+K+GGRVVVLNTKNLPLVGEVG GADLVR+D ++MCSP
Sbjct: 181 EPKKGDRKGFVLNCLEAPLDVDIKDGGRVVVLNTKNLPLVGEVGFGADLVRIDGHSMCSP 240

Query: 241 GFSCDSALQVTYIVKGSGRAEVVGVDGKKVLETRVKAGDLFIVPKFFVVSKIRDPEGMEW 300
           GFSCDSALQVTYIV GSGR ++VG DGK+VLET VKAG LFIVP+FFVVSKI D +G+ W
Sbjct: 241 GFSCDSALQVTYIVGGSGRVQIVGADGKRVLETHVKAGVLFIVPRFFVVSKIADSDGLSW 300

Query: 301 FSIITTPNPVFTHLAGNIGVWKSLSLEVIQATFDVDIDLVKNFSCKRASDAIFFPPSN 359
           FSI+TTP+P+FTHLAG   VWK+LS EV+QA F VD ++ K F  KR SDAIFF PSN
Sbjct: 301 FSIVTTPDPIFTHLAGRTSVWKALSPEVLQAAFKVDPEVEKAFRSKRTSDAIFFSPSN 356

[Close]

BLAST of Lsi11G012810 vs. TAIR 10
Match: AT1G07750.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 530.4 bits (1365), Expect = 1.1e-150
Identity = 254/358 (70.95%), Postives = 303/358 (84.64%), Query Frame = 0


Query: 1   MEIDLTPQLSKKVYGSGSDGGSYYSWSLKELTMLREGNIGASKVALKKNGFALPHYFDST 60
           ME+DLTP+L KKVY  G DGGSY +W  +EL ML++GNIGA+K+AL+KNGFA+P Y DS+
Sbjct: 1   MELDLTPKLPKKVY--GGDGGSYSAWCPEELPMLKQGNIGAAKLALEKNGFAVPRYSDSS 60

Query: 61  KVAYVLQGNGVAGIIMSESEEKIIAIKKGDAIALPFGMVTWWFNKEDTDLVVLFLGDTSK 120
           KVAYVLQG+G AGI++ E EEK+IAIK+GD+IALPFG+VTWWFN ED +LV+LFLG+T K
Sbjct: 61  KVAYVLQGSGTAGIVLPEKEEKVIAIKQGDSIALPFGVVTWWFNNEDPELVILFLGETHK 120

Query: 121 AHKSGEFTNFFLTGANGIFTGFSTEFVERAWDMDEVSVKCMVKNQTGTGIVKLKEGTKMP 180
            HK+G+FT F+LTG NGIFTGFSTEFV RAWD+DE +VK +V +QTG GIVKL  G KMP
Sbjct: 121 GHKAGQFTEFYLTGTNGIFTGFSTEFVGRAWDLDENTVKKLVGSQTGNGIVKLDAGFKMP 180

Query: 181 EAKKEDRNGMAVNCEEAPLDVDVKNGGRVVVLNTKNLPLVGEVGLGADLVRLDRNAMCSP 240
           + K+E+R G  +NC EAPLDVD+K+GGRVVVLNTKNLPLVGEVG GADLVR+D ++MCSP
Sbjct: 181 QPKEENRAGFVLNCLEAPLDVDIKDGGRVVVLNTKNLPLVGEVGFGADLVRIDAHSMCSP 240

Query: 241 GFSCDSALQVTYIVKGSGRAEVVGVDGKKVLETRVKAGDLFIVPKFFVVSKIRDPEGMEW 300
           GFSCDSALQVTYIV GSGR +VVG DGK+VLET +KAG LFIVP+FFVVSKI D +GM W
Sbjct: 241 GFSCDSALQVTYIVGGSGRVQVVGGDGKRVLETHIKAGSLFIVPRFFVVSKIADADGMSW 300

Query: 301 FSIITTPNPVFTHLAGNIGVWKSLSLEVIQATFDVDIDLVKNFSCKRASDAIFFPPSN 359
           FSI+TTP+P+FTHLAGN  VWKSLS EV+QA F V  ++ K+F   R S AIFFPPSN
Sbjct: 301 FSIVTTPDPIFTHLAGNTSVWKSLSPEVLQAAFKVAPEVEKSFRSTRTSSAIFFPPSN 356

[Close]

BLAST of Lsi11G012810 vs. TAIR 10
Match: AT1G03890.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 115.5 bits (288), Expect = 8.6e-26
Identity = 89/384 (23.18%), Postives = 162/384 (42.19%), Query Frame = 0


Query: 34  LREGNIGASKVALKKNGFALPHYFDSTKVAYVLQGNGVAGIIMS---------------- 93
           LR   +  +++ L+ N   LP +F    +AYV+QG GV G I S                
Sbjct: 67  LRCAGVTVARITLQPNSIFLPAFFSPPALAYVVQGEGVMGTIASGCPETFAEVEGSSGRG 126

Query: 94  ----------ESEEKIIAIKKGDAIALPFGMVTWWFNKEDTDLVVLFLGD-TSKAHKSGE 153
                     +  +K+   ++GD  A   G+  WW+N+ D+D V++ + D T++ ++  +
Sbjct: 127 GGGDPGRRFEDMHQKLENFRRGDVFASLAGVSQWWYNRGDSDAVIVIVLDVTNRENQLDQ 186

Query: 154 FTNFF-LTGA--------------NGIFTGFSTEFVERAWDMDEVSVKCMVKNQTGTGIV 213
               F L G+              N  F+GF    +  A+ ++  + K +   +   G +
Sbjct: 187 VPRMFQLAGSRTQEEEQPLTWPSGNNAFSGFDPNIIAEAFKINIETAKQLQNQKDNRGNI 246

Query: 214 KLKEGT---KMPEAKKEDRNGMAVNCEEAPLDVDV--------------KNGGRVVVLNT 273
               G     +P  ++  ++G+A   EE      +                 GR+  LN+
Sbjct: 247 IRANGPLHFVIPPPREWQQDGIANGIEETYCTAKIHENIDDPERSDHFSTRAGRISTLNS 306

Query: 274 KNLPLVGEVGLGADLVRLDRNAMCSPGFSCDSALQVTYIVKGSGRAEVVGVDGKKVLETR 333
            NLP++  V L A    L    M  P ++  +A  V Y+  G  + +VV  +G+ V   +
Sbjct: 307 LNLPVLRLVRLNALRGYLYSGGMVLPQWTA-NAHTVLYVTGGQAKIQVVDDNGQSVFNEQ 366

Query: 334 VKAGDLFIVPKFFVVSKIRDPEGMEWFSIITTPNPVFTHLAGNIGVWKSLSLEVIQATFD 359
           V  G + ++P+ F VSK     G EW S  T  N     L+G     +++ ++VI+A++ 
Sbjct: 367 VGQGQIIVIPQGFAVSKTAGETGFEWISFKTNDNAYINTLSGQTSYLRAVPVDVIKASYG 426

[Close]

BLAST of Lsi11G012810 vs. TAIR 10
Match: AT1G03880.1 (cruciferin 2 )

HSP 1 Score: 99.8 bits (247), Expect = 4.9e-21
Identity = 91/391 (23.27%), Postives = 159/391 (40.66%), Query Frame = 0


Query: 18  SDGGSYYSWSLKELTMLREGNIGASKVALKKNGFALPHYFDSTKVAYVLQGNGVAGIIM- 77
           S+GG    W       LR       +  ++  G  LP + ++ K+ +V+ G G+ G ++ 
Sbjct: 46  SEGGRIEVWD-HHAPQLRCSGFAFERFVIEPQGLFLPTFLNAGKLTFVVHGRGLMGRVIP 105

Query: 78  ------------------------SESEEKIIAIKKGDAIALPFGMVTWWFNKEDTDLVV 137
                                    +  +K+  ++ GD IA P G+  W++N  +  L++
Sbjct: 106 GCAETFMESPVFGEGQGQGQSQGFRDMHQKVEHLRCGDTIATPSGVAQWFYNNGNEPLIL 165

Query: 138 LFLGD--TSKAHKSGEFTNFFLTG----------------ANGIFTGFSTEFVERAWDMD 197
           +   D  +++         F + G                 N IF GF+ E + +A+ ++
Sbjct: 166 VAAADLASNQNQLDRNLRPFLIAGNNPQGQEWLQGRKQQKQNNIFNGFAPEILAQAFKIN 225

Query: 198 EVSVKCMVKNQTGTG-IVKLK-------------EGTKMPEAKKEDRNGM-----AVNCE 257
             + + +   Q   G IVK+              EG + P    E  NG+      + C 
Sbjct: 226 VETAQQLQNQQDNRGNIVKVNGPFGVIRPPLRRGEGGQQPH---EIANGLEETLCTMRCT 285

Query: 258 E---APLDVDV--KNGGRVVVLNTKNLPLVGEVGLGADLVRLDRNAMCSPGFSCDSALQV 317
           E    P D DV   + G +  LN+ NLP++  + L A    + +NAM  P ++  +A   
Sbjct: 286 ENLDDPSDADVYKPSLGYISTLNSYNLPILRLLRLSALRGSIRKNAMVLPQWNV-NANAA 345

Query: 318 TYIVKGSGRAEVVGVDGKKVLETRVKAGDLFIVPKFFVVSKIRDPEGMEWFSIITTPNPV 342
            Y+  G    ++V  +G++V +  + +G L +VP+ F V K    E  EW    T  N  
Sbjct: 346 LYVTNGKAHIQMVNDNGERVFDQEISSGQLLVVPQGFSVMKHAIGEQFEWIEFKTNENAQ 405

[Close]

BLAST of Lsi11G012810 vs. TAIR 10
Match: AT4G28520.1 (cruciferin 3 )

HSP 1 Score: 82.8 bits (203), Expect = 6.1e-16
Identity = 73/300 (24.33%), Postives = 125/300 (41.67%), Query Frame = 0


Query: 78  ESEEKIIAIKKGDAIALPFGMVTWWFNKEDTDLVVLFLGDTSKAHKSGEFTN--FFLTGA 137
           +  +K+  +++GD  A   G   W +N  +  LV++ L D +      +     F L G 
Sbjct: 191 DMHQKVEHVRRGDVFANTPGSAHWIYNSGEQPLVIIALLDIANYQNQLDRNPRVFHLAGN 250

Query: 138 N---------------GIFTGFSTEFVERAWDMDEVSVKCMVKNQTGTG-IVKLK----- 197
           N                +++GF  + + +A  +D    + +   Q   G IV++K     
Sbjct: 251 NQQGGFGGSQQQQEQKNLWSGFDAQVIAQALKIDVQLAQQLQNQQDSRGNIVRVKGPFQV 310

Query: 198 ---------EGTKMPEAKKEDRNGMAVNC----------EEAPLDVDVKNGGRVVVLNTK 257
                    E  +    +    NG+              + A  DV   + GRV  +N+ 
Sbjct: 311 VRPPLRQPYESEEWRHPRSPQGNGLEETICSMRSHENIDDPARADVYKPSLGRVTSVNSY 370

Query: 258 NLPLVGEVGLGADLVRLDRNAMCSPGFSCDSALQVTYIVKGSGRAEVVGVDGKKVLETRV 317
            LP++  V L A    L  NAM  P ++  +A ++ Y   G GR +VV  +G+ VL+ +V
Sbjct: 371 TLPILEYVRLSATRGVLQGNAMVLPKYNM-NANEILYCTGGQGRIQVVNDNGQNVLDQQV 430

Query: 318 KAGDLFIVPKFFVVSKIRDPEGMEWFSIITTPNPVFTHLAGNIGVWKSLSLEVIQATFDV 336
           + G L ++P+ F           EW S  T  N + + LAG   + ++L LEVI   F +
Sbjct: 431 QKGQLVVIPQGFAYVVQSHGNKFEWISFKTNENAMISTLAGRTSLLRALPLEVISNGFQI 489

The following BLAST results are available for this feature:

BLAST of Lsi11G012810 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9XHP0	2.9e-26	21.76	11S globulin seed storage protein 2 OS=Sesamum indicum OX=4182 PE=1 SV=1	[more]
Q8GZP6	1.2e-24	23.97	11S globulin seed storage protein Ana o 2.0101 (Fragment) OS=Anacardium occident...	[more]
Q9ZWA9	1.2e-24	23.18	12S seed storage protein CRD OS=Arabidopsis thaliana OX=3702 GN=CRD PE=1 SV=1	[more]
P07730	2.1e-24	23.23	Glutelin type-A 2 OS=Oryza sativa subsp. japonica OX=39947 GN=GLUA2 PE=1 SV=1	[more]
P14614	2.7e-24	23.86	Glutelin type-B 4 OS=Oryza sativa subsp. japonica OX=39947 GN=GLUB4 PE=1 SV=1	[more]

BLAST of Lsi11G012810 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K666	2.0e-179	88.55	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G281380 PE=3 SV=1	[more]
A0A5A7UAB0	4.4e-179	88.55	Glutelin type-B 5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold6...	[more]
A0A1S3CG59	4.4e-179	88.55	glutelin type-B 5-like OS=Cucumis melo OX=3656 GN=LOC103500083 PE=3 SV=1	[more]
A0A6J1EX25	2.8e-178	88.52	glutelin type-D 1-like OS=Cucurbita moschata OX=3662 GN=LOC111439037 PE=3 SV=1	[more]
A0A6J1IH21	4.9e-178	88.24	glutelin type-D 1-like OS=Cucurbita maxima OX=3661 GN=LOC111477153 PE=3 SV=1	[more]

BLAST of Lsi11G012810 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6592225.1	2.4e-179	89.08	Glutelin type-D 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7025082.1...	[more]
XP_004150394.1	4.1e-179	88.55	glutelin type-D 1 [Cucumis sativus] >KGN44409.1 hypothetical protein Csa_015780 ...	[more]
XP_023535755.1	6.9e-179	88.80	glutelin type-D 1-like [Cucurbita pepo subsp. pepo]	[more]
XP_008461502.1	9.1e-179	88.55	PREDICTED: glutelin type-B 5-like [Cucumis melo] >KAA0052863.1 glutelin type-B 5...	[more]
XP_022932542.1	5.9e-178	88.52	glutelin type-D 1-like [Cucurbita moschata]	[more]

BLAST of Lsi11G012810 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G28680.1	6.5e-151	71.51	RmlC-like cupins superfamily protein	[more]
AT1G07750.1	1.1e-150	70.95	RmlC-like cupins superfamily protein	[more]
AT1G03890.1	8.6e-26	23.18	RmlC-like cupins superfamily protein	[more]
AT1G03880.1	4.9e-21	23.27	cruciferin 2	[more]
AT4G28520.1	6.1e-16	24.33	cruciferin 3	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006044	11-S seed storage protein, plant	PRINTS	PR00439	11SGLOBULIN	coord: 260..276 score: 26.96 coord: 318..335 score: 20.42 coord: 213..233 score: 29.85 coord: 278..293 score: 37.69 coord: 296..314 score: 30.27
IPR006045	Cupin 1	SMART	SM00835	Cupin_1_3	coord: 199..341 e-value: 5.9E-17 score: 72.3 coord: 11..159 e-value: 7.6E-28 score: 108.5
IPR006045	Cupin 1	PFAM	PF00190	Cupin_1	coord: 201..336 e-value: 1.6E-21 score: 76.4 coord: 18..156 e-value: 2.5E-26 score: 92.1
IPR014710	RmlC-like jelly roll fold	GENE3D	2.60.120.10	Jelly Rolls	coord: 21..186 e-value: 4.4E-33 score: 116.4
IPR014710	RmlC-like jelly roll fold	GENE3D	2.60.120.10	Jelly Rolls	coord: 195..358 e-value: 7.1E-47 score: 160.8
None	No IPR available	PANTHER	PTHR31189:SF51	SUBFAMILY NOT NAMED	coord: 2..348
None	No IPR available	PANTHER	PTHR31189	OS03G0336100 PROTEIN-RELATED	coord: 2..348
None	No IPR available	CDD	cd02243	cupin_11S_legumin_C	coord: 200..355 e-value: 1.10812E-72 score: 220.808
None	No IPR available	CDD	cd02242	cupin_11S_legumin_N	coord: 4..175 e-value: 1.26043E-61 score: 194.726
IPR011051	RmlC-like cupin domain superfamily	SUPERFAMILY	51182	RmlC-like cupins	coord: 18..345

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi11G012810.1	Lsi11G012810.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010431	seed maturation
cellular_component	GO:0005783	endoplasmic reticulum
cellular_component	GO:0000326	protein storage vacuole
molecular_function	GO:0045735	nutrient reservoir activity