Lsi11G012320 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi11G012320
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	O-methyltransferase family protein
Location	chr11: 20912338 .. 20912724 (-)
RNA-Seq Expression	Lsi11G012320
Synteny	Lsi11G012320

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAGTAATAAAATGTGCCATTGAGCTGAAAATTGGAGACGTCATTGAAAGCCATGGAGGTTCCATGACACTCTCCCAGCTATCTTCTTCTTTGAAGTGTTGTCCTTCGCTTCTACACCGAATCTTGCGGTTCCTTGTTCATCGAGGCATTTTCAAAGAAGAAAAAACCACAGAAAGCTACTCTCAAACGCCCATGTCTCGGTTCCTCACAACTAGTGGACCAAATAGCTTGGCTCCCTTACATCTCTTGGAGAGCAGCCCTGTGATGGTTGAGCCATGGCACTACTTGAGTTCATGTCTTAAAGCCAAAGGGAGTCAGCCAATTGAGATGGCTCATGGGATGGACGCGTGGAGTTATGCTTGCAGCCAATCCATGGTTCAATAG

mRNA sequence

ATGGCAGTAATAAAATGTGCCATTGAGCTGAAAATTGGAGACGTCATTGAAAGCCATGGAGGTTCCATGACACTCTCCCAGCTATCTTCTTCTTTGAAGTGTTGTCCTTCGCTTCTACACCGAATCTTGCGGTTCCTTGTTCATCGAGGCATTTTCAAAGAAGAAAAAACCACAGAAAGCTACTCTCAAACGCCCATGTCTCGGTTCCTCACAACTAGTGGACCAAATAGCTTGGCTCCCTTACATCTCTTGGAGAGCAGCCCTGTGATGGTTGAGCCATGGCACTACTTGAGTTCATGTCTTAAAGCCAAAGGGAGTCAGCCAATTGAGATGGCTCATGGGATGGACGCGTGGAGTTATGCTTGCAGCCAATCCATGGTTCAATAG

Coding sequence (CDS)

ATGGCAGTAATAAAATGTGCCATTGAGCTGAAAATTGGAGACGTCATTGAAAGCCATGGAGGTTCCATGACACTCTCCCAGCTATCTTCTTCTTTGAAGTGTTGTCCTTCGCTTCTACACCGAATCTTGCGGTTCCTTGTTCATCGAGGCATTTTCAAAGAAGAAAAAACCACAGAAAGCTACTCTCAAACGCCCATGTCTCGGTTCCTCACAACTAGTGGACCAAATAGCTTGGCTCCCTTACATCTCTTGGAGAGCAGCCCTGTGATGGTTGAGCCATGGCACTACTTGAGTTCATGTCTTAAAGCCAAAGGGAGTCAGCCAATTGAGATGGCTCATGGGATGGACGCGTGGAGTTATGCTTGCAGCCAATCCATGGTTCAATAG

Protein sequence

MAVIKCAIELKIGDVIESHGGSMTLSQLSSSLKCCPSLLHRILRFLVHRGIFKEEKTTESYSQTPMSRFLTTSGPNSLAPLHLLESSPVMVEPWHYLSSCLKAKGSQPIEMAHGMDAWSYACSQSMVQ

Homology

[Close]

BLAST of Lsi11G012320 vs. ExPASy Swiss-Prot
Match: Q9T003 (Acetylserotonin O-methyltransferase OS=Arabidopsis thaliana OX=3702 GN=ASMT PE=1 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 1.9e-20
Identity = 61/133 (45.86%), Postives = 82/133 (61.65%), Query Frame = 0


Query: 1   MAVIKCAIELKIGDVIESHGGS--MTLSQLSSSLKCCPSLLHRILRFLVHRGIFKEEKTT 60
           +A  KCAI+LKI + IE+H  S  +TL++LSS++   PS L RI+RFLVH+GIFKE  T 
Sbjct: 43  IAAAKCAIDLKIPEAIENHPSSQPVTLAELSSAVSASPSHLRRIMRFLVHQGIFKEIPTK 102

Query: 61  E----SYSQTPMSR--FLTTSGPNSLAPLHLLESSPVMVEPWHYLSSCLKA--KGS--QP 120
           +     Y  TP+SR   +T     SLAP  L E++P M+ PW  LSS + +   GS   P
Sbjct: 103 DGLATGYVNTPLSRRLMITRRDGKSLAPFVLFETTPEMLAPWLRLSSVVSSPVNGSTPPP 162

Query: 121 IEMAHGMDAWSYA 122
            +  HG D WS+A
Sbjct: 163 FDAVHGKDVWSFA 175

[Close]

BLAST of Lsi11G012320 vs. ExPASy Swiss-Prot
Match: Q6WUC2 ((R,S)-reticuline 7-O-methyltransferase OS=Papaver somniferum OX=3469 GN=7OMT PE=1 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 5.1e-18
Identity = 53/129 (41.09%), Postives = 76/129 (58.91%), Query Frame = 0


Query: 4   IKCAIELKIGDVIESHGGSMTLSQLSSSLKC-CPS------LLHRILRFLVHRGIFKEEK 63
           +KCA+EL I D+I SHG  +T+S++  SLK   PS       L RI+R LVH+ +F  E 
Sbjct: 27  LKCAVELGIPDIINSHGRPVTISEIVDSLKTNTPSSSPNIDYLTRIMRLLVHKRLFTSEL 86

Query: 64  TTES----YSQTPMSRFLTTSGPNSLAPLHLLESSPVMVEPWHYLSSCLKAKGSQPIEMA 122
             ES    Y+ T  S++L      +L+PL L E++P++++PW YL  C + K S P E A
Sbjct: 87  HQESNQLLYNLTRSSKWLLKDSKFNLSPLVLWETNPILLKPWQYLGKCAQEK-SSPFERA 146

[Close]

BLAST of Lsi11G012320 vs. ExPASy Swiss-Prot
Match: B0ZB55 (Desmethylxanthohumol 6'-O-methyltransferase OS=Humulus lupulus OX=3486 GN=OMT1 PE=1 SV=2)

HSP 1 Score: 83.6 bits (205), Expect = 1.8e-15
Identity = 47/131 (35.88%), Postives = 76/131 (58.02%), Query Frame = 0


Query: 4   IKCAIELKIGDVIESHGGSMTLSQLSSSLK------CCPSL--LHRILRFLVHRGIFKE- 63
           +KCAIEL+I D+I SHG  +TLSQ++S ++        P++  L RI+RFLV + IF E 
Sbjct: 23  LKCAIELRIADIIHSHGKPITLSQIASGIRSNSNSSISPNIPYLSRIMRFLVRKNIFTEH 82

Query: 64  ----EKTTESYSQTPMSRFLTTSGPNSLAPLHLLESSPVMVEPWHYLSSCLKAKGSQPIE 122
               ++    Y  +  SR+L     +SLAP+ L+++ P+ +  WH+L   ++   S   E
Sbjct: 83  QEDNDEVISLYGLSDSSRWLLRDFKSSLAPMVLMQTHPLSMAVWHFLEDYVR-NSSNTFE 142

[Close]

BLAST of Lsi11G012320 vs. ExPASy Swiss-Prot
Match: B0ZB56 (Xanthohumol 4-O-methyltransferase OS=Humulus lupulus OX=3486 GN=OMT2 PE=1 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 2.2e-13
Identity = 48/124 (38.71%), Postives = 68/124 (54.84%), Query Frame = 0


Query: 3   VIKCAIELKIGDVIESHGGSMTLSQLSSSLKCCPSL----LHRILRFLVHRGIFKEEKTT 62
           V+KCA+EL+I D++ SH   +TL+Q++SS+   PSL    L RI+R LV R IF + K+ 
Sbjct: 31  VMKCALELRIPDIVHSHSAPITLAQIASSVPDSPSLNLSYLSRIMRLLVRRKIFSQHKSL 90

Query: 63  ES----YSQTPMSRFL----TTSGPNSLAPLHLLESSPVMVEPWHYLSSCLKAKGSQPIE 115
           +     Y  T  SR L    T     +LAP     + P +  PW  L+ C+K +G    E
Sbjct: 91  DGEEVLYGPTHSSRLLLSKTTLPDQVTLAPFVAFMTHPYLSAPWSCLARCVK-EGGNGFE 150

[Close]

BLAST of Lsi11G012320 vs. ExPASy Swiss-Prot
Match: Q6WUC1 ((RS)-norcoclaurine 6-O-methyltransferase OS=Papaver somniferum OX=3469 GN=6OMT PE=1 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 1.0e-10
Identity = 39/122 (31.97%), Postives = 63/122 (51.64%), Query Frame = 0


Query: 3   VIKCAIELKIGDVIESHGGSMTLSQLSSSLKCCPSL----LHRILRFLVHRGIFKEEKTT 62
           V+KCA++L+I + + ++   M+LS+L+S L     +    L RI+R+LVH  +FK + TT
Sbjct: 27  VLKCAVQLEIAETLHNNVKPMSLSELASKLPVAQPVNEDRLFRIMRYLVHMELFKIDATT 86

Query: 63  ESYSQTPMSRFLTTSGPNSLAPLHLLESSPVMVEPWHYLSSCLKAKGSQPIEMAHGMDAW 121
           + YS  P +++L      S+    L  +    + PWH+L   L        E A G   W
Sbjct: 87  QKYSLAPPAKYLLRGWEKSMVDSILCINDKDFLAPWHHLGDGLTG-NCDAFEKALGKSIW 146

[Close]

BLAST of Lsi11G012320 vs. ExPASy TrEMBL
Match: A0A0A0K0Q2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G018720 PE=3 SV=1)

HSP 1 Score: 206.5 bits (524), Expect = 6.8e-50
Identity = 102/127 (80.31%), Postives = 110/127 (86.61%), Query Frame = 0


Query: 1   MAVIKCAIELKIGDVIESHGGSMTLSQLSSSLKCCPSLLHRILRFLVHRGIFKEEKTTES 60
           MAVIKCAIELKIGDVIESHG SMTLSQLSSSL C P LLHRILRFLVHRGIFKEEKTT+ 
Sbjct: 35  MAVIKCAIELKIGDVIESHGDSMTLSQLSSSLNCSPLLLHRILRFLVHRGIFKEEKTTKC 94

Query: 61  YSQTPMSRFLTTSGPNSLAPLHLLESSPVMVEPWHYLSSCLKA----KGSQPIEMAHGMD 120
           YS TPMSR LTT+GPNS+APLHLLESSPVM+ PWH LS+C+KA     GSQP EMAHGMD
Sbjct: 95  YSHTPMSRLLTTTGPNSMAPLHLLESSPVMIAPWHNLSACVKASDHENGSQPFEMAHGMD 154

Query: 121 AWSYACS 124
            W+YA +
Sbjct: 155 LWTYAAA 161

[Close]

BLAST of Lsi11G012320 vs. ExPASy TrEMBL
Match: A0A1S3BHY1 ((RS)-norcoclaurine 6-O-methyltransferase-like OS=Cucumis melo OX=3656 GN=LOC103490251 PE=3 SV=1)

HSP 1 Score: 204.1 bits (518), Expect = 3.4e-49
Identity = 99/127 (77.95%), Postives = 111/127 (87.40%), Query Frame = 0


Query: 1   MAVIKCAIELKIGDVIESHGGSMTLSQLSSSLKCCPSLLHRILRFLVHRGIFKEEKTTES 60
           MAV+KCAIELKIGDVIESHG SMTLSQLSSSL C P LLHRILRFLVHRGIFKEEK+++S
Sbjct: 35  MAVVKCAIELKIGDVIESHGDSMTLSQLSSSLSCSPLLLHRILRFLVHRGIFKEEKSSKS 94

Query: 61  YSQTPMSRFLTTSGPNSLAPLHLLESSPVMVEPWHYLSSCLKA----KGSQPIEMAHGMD 120
           YS TPMSR LTT+GPNS+AP+HLLESSPVM+ PWH LS+C+KA     GSQP EMAHGMD
Sbjct: 95  YSHTPMSRLLTTTGPNSMAPIHLLESSPVMIAPWHNLSACVKANGHENGSQPFEMAHGMD 154

Query: 121 AWSYACS 124
            W+YA +
Sbjct: 155 LWTYAAA 161

[Close]

BLAST of Lsi11G012320 vs. ExPASy TrEMBL
Match: A0A5D3DI94 ((RS)-norcoclaurine 6-O-methyltransferase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold142G004200 PE=3 SV=1)

HSP 1 Score: 202.6 bits (514), Expect = 9.9e-49
Identity = 98/127 (77.17%), Postives = 111/127 (87.40%), Query Frame = 0


Query: 1   MAVIKCAIELKIGDVIESHGGSMTLSQLSSSLKCCPSLLHRILRFLVHRGIFKEEKTTES 60
           MAV+KCAIELKIGDVIESHG S+TLSQLSSSL C P LLHRILRFLVHRGIFKEEK+++S
Sbjct: 35  MAVVKCAIELKIGDVIESHGDSITLSQLSSSLSCSPLLLHRILRFLVHRGIFKEEKSSKS 94

Query: 61  YSQTPMSRFLTTSGPNSLAPLHLLESSPVMVEPWHYLSSCLKA----KGSQPIEMAHGMD 120
           YS TPMSR LTT+GPNS+AP+HLLESSPVM+ PWH LS+C+KA     GSQP EMAHGMD
Sbjct: 95  YSHTPMSRLLTTTGPNSMAPIHLLESSPVMIAPWHNLSACVKANGHENGSQPFEMAHGMD 154

Query: 121 AWSYACS 124
            W+YA +
Sbjct: 155 LWTYAAA 161

[Close]

BLAST of Lsi11G012320 vs. ExPASy TrEMBL
Match: A0A6J1GLV0 (xanthohumol 4'-O-methyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111455162 PE=3 SV=1)

HSP 1 Score: 177.9 bits (450), Expect = 2.6e-41
Identity = 91/127 (71.65%), Postives = 103/127 (81.10%), Query Frame = 0


Query: 1   MAVIKCAIELKIGDVIESHGGSMTLSQLSSSLKCCPSLLHRILRFLVHRGIFKEEKTTES 60
           MAVIKCAIELKIGD IE+HGG MTL+ LSSSL C P LLHRILRFL+HRGIFKEE  T+S
Sbjct: 35  MAVIKCAIELKIGDTIEAHGGLMTLTDLSSSLNCSPLLLHRILRFLIHRGIFKEE-PTKS 94

Query: 61  YSQTPMSRFLTTSGPNSLAPLHLLESSPVMVEPWHYLSSCLKAK--GSQPIEMAHGMDAW 120
           Y+QTPMSR LT+SG +SLAP+H+LESS VM+ PWH LS+ LKA   GSQP EMAHG D W
Sbjct: 95  YAQTPMSRLLTSSGAHSLAPMHVLESSHVMLAPWHNLSAYLKANDGGSQPFEMAHGKDLW 154

Query: 121 SYACSQS 126
            Y  + S
Sbjct: 155 RYCAANS 160

[Close]

BLAST of Lsi11G012320 vs. ExPASy TrEMBL
Match: A0A6J1HVJ9 (xanthohumol 4'-O-methyltransferase-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111468232 PE=3 SV=1)

HSP 1 Score: 169.5 bits (428), Expect = 9.3e-39
Identity = 87/127 (68.50%), Postives = 101/127 (79.53%), Query Frame = 0


Query: 1   MAVIKCAIELKIGDVIESHGGSMTLSQLSSSLKCCPSLLHRILRFLVHRGIFKEEKTTES 60
           MAVIKCAIELKI D I++HGG MTL +LSSSL C P LL RILRFL+HRGIFKEE   +S
Sbjct: 1   MAVIKCAIELKIADTIDAHGGLMTLPELSSSLNCSPLLLRRILRFLIHRGIFKEE-PIKS 60

Query: 61  YSQTPMSRFLTTSGPNSLAPLHLLESSPVMVEPWHYLSSCLKAK--GSQPIEMAHGMDAW 120
           Y+QTPMSR LT+SG +SLAP+H+LESS VM+ PWH LS+ LKA   G+QP EMAHG D W
Sbjct: 61  YAQTPMSRLLTSSGAHSLAPIHVLESSLVMLAPWHNLSAYLKANNGGTQPFEMAHGKDLW 120

Query: 121 SYACSQS 126
           SY  + S
Sbjct: 121 SYCAANS 126

[Close]

BLAST of Lsi11G012320 vs. NCBI nr
Match: XP_004144871.1 (acetylserotonin O-methyltransferase [Cucumis sativus] >KGN43290.1 hypothetical protein Csa_020497 [Cucumis sativus])

HSP 1 Score: 206.5 bits (524), Expect = 1.4e-49
Identity = 102/127 (80.31%), Postives = 110/127 (86.61%), Query Frame = 0


Query: 1   MAVIKCAIELKIGDVIESHGGSMTLSQLSSSLKCCPSLLHRILRFLVHRGIFKEEKTTES 60
           MAVIKCAIELKIGDVIESHG SMTLSQLSSSL C P LLHRILRFLVHRGIFKEEKTT+ 
Sbjct: 35  MAVIKCAIELKIGDVIESHGDSMTLSQLSSSLNCSPLLLHRILRFLVHRGIFKEEKTTKC 94

Query: 61  YSQTPMSRFLTTSGPNSLAPLHLLESSPVMVEPWHYLSSCLKA----KGSQPIEMAHGMD 120
           YS TPMSR LTT+GPNS+APLHLLESSPVM+ PWH LS+C+KA     GSQP EMAHGMD
Sbjct: 95  YSHTPMSRLLTTTGPNSMAPLHLLESSPVMIAPWHNLSACVKASDHENGSQPFEMAHGMD 154

Query: 121 AWSYACS 124
            W+YA +
Sbjct: 155 LWTYAAA 161

[Close]

BLAST of Lsi11G012320 vs. NCBI nr
Match: XP_008447905.1 (PREDICTED: (RS)-norcoclaurine 6-O-methyltransferase-like [Cucumis melo])

HSP 1 Score: 204.1 bits (518), Expect = 7.0e-49
Identity = 99/127 (77.95%), Postives = 111/127 (87.40%), Query Frame = 0


Query: 1   MAVIKCAIELKIGDVIESHGGSMTLSQLSSSLKCCPSLLHRILRFLVHRGIFKEEKTTES 60
           MAV+KCAIELKIGDVIESHG SMTLSQLSSSL C P LLHRILRFLVHRGIFKEEK+++S
Sbjct: 35  MAVVKCAIELKIGDVIESHGDSMTLSQLSSSLSCSPLLLHRILRFLVHRGIFKEEKSSKS 94

Query: 61  YSQTPMSRFLTTSGPNSLAPLHLLESSPVMVEPWHYLSSCLKA----KGSQPIEMAHGMD 120
           YS TPMSR LTT+GPNS+AP+HLLESSPVM+ PWH LS+C+KA     GSQP EMAHGMD
Sbjct: 95  YSHTPMSRLLTTTGPNSMAPIHLLESSPVMIAPWHNLSACVKANGHENGSQPFEMAHGMD 154

Query: 121 AWSYACS 124
            W+YA +
Sbjct: 155 LWTYAAA 161

[Close]

BLAST of Lsi11G012320 vs. NCBI nr
Match: KAA0040348.1 ((RS)-norcoclaurine 6-O-methyltransferase-like [Cucumis melo var. makuwa] >TYK23356.1 (RS)-norcoclaurine 6-O-methyltransferase-like [Cucumis melo var. makuwa])

HSP 1 Score: 202.6 bits (514), Expect = 2.0e-48
Identity = 98/127 (77.17%), Postives = 111/127 (87.40%), Query Frame = 0


Query: 1   MAVIKCAIELKIGDVIESHGGSMTLSQLSSSLKCCPSLLHRILRFLVHRGIFKEEKTTES 60
           MAV+KCAIELKIGDVIESHG S+TLSQLSSSL C P LLHRILRFLVHRGIFKEEK+++S
Sbjct: 35  MAVVKCAIELKIGDVIESHGDSITLSQLSSSLSCSPLLLHRILRFLVHRGIFKEEKSSKS 94

Query: 61  YSQTPMSRFLTTSGPNSLAPLHLLESSPVMVEPWHYLSSCLKA----KGSQPIEMAHGMD 120
           YS TPMSR LTT+GPNS+AP+HLLESSPVM+ PWH LS+C+KA     GSQP EMAHGMD
Sbjct: 95  YSHTPMSRLLTTTGPNSMAPIHLLESSPVMIAPWHNLSACVKANGHENGSQPFEMAHGMD 154

Query: 121 AWSYACS 124
            W+YA +
Sbjct: 155 LWTYAAA 161

[Close]

BLAST of Lsi11G012320 vs. NCBI nr
Match: XP_038888743.1 (acetylserotonin O-methyltransferase-like [Benincasa hispida])

HSP 1 Score: 202.2 bits (513), Expect = 2.7e-48
Identity = 100/125 (80.00%), Postives = 108/125 (86.40%), Query Frame = 0


Query: 1   MAVIKCAIELKIGDVIESHGGSMTLSQLSSSLKCCPSLLHRILRFLVHRGIFKEEKTTES 60
           MAVIKCAIELKIGD IES+GGSMTLS+LSSSL   P LLHR+LRFLVHRGIFKEEK T+S
Sbjct: 35  MAVIKCAIELKIGDTIESNGGSMTLSELSSSLNSSPLLLHRVLRFLVHRGIFKEEKATQS 94

Query: 61  YSQTPMSRFLTTSGPNSLAPLHLLESSPVMVEPWHYLSSCLKAKG----SQPIEMAHGMD 120
           YSQTPMSR LTT+GPNSLAP+HLLESSP MV PWHYLS+CLKA      SQP E+AHGMD
Sbjct: 95  YSQTPMSRLLTTTGPNSLAPIHLLESSPAMVAPWHYLSACLKANDHENESQPFELAHGMD 154

Query: 121 AWSYA 122
            WSYA
Sbjct: 155 LWSYA 159

[Close]

BLAST of Lsi11G012320 vs. NCBI nr
Match: KAG6572137.1 (Acetylserotonin O-methyltransferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 178.7 bits (452), Expect = 3.2e-41
Identity = 91/127 (71.65%), Postives = 104/127 (81.89%), Query Frame = 0


Query: 1   MAVIKCAIELKIGDVIESHGGSMTLSQLSSSLKCCPSLLHRILRFLVHRGIFKEEKTTES 60
           MAVIKCAIELKIGD IE+HGG MTL++LSSSL C P LLHRILRFL+HRGIFKEE  T+S
Sbjct: 35  MAVIKCAIELKIGDTIEAHGGLMTLTELSSSLNCSPLLLHRILRFLIHRGIFKEE-PTKS 94

Query: 61  YSQTPMSRFLTTSGPNSLAPLHLLESSPVMVEPWHYLSSCLKAK--GSQPIEMAHGMDAW 120
           Y+QTPMSR LT+SG +SLAP+H+LESS VM+ PWH LS+ LKA   GSQP EMAHG D W
Sbjct: 95  YAQTPMSRLLTSSGAHSLAPMHVLESSHVMLAPWHNLSAYLKANDGGSQPFEMAHGKDLW 154

Query: 121 SYACSQS 126
            Y  + S
Sbjct: 155 RYCAANS 160

[Close]

BLAST of Lsi11G012320 vs. TAIR 10
Match: AT4G35160.1 (O-methyltransferase family protein )

HSP 1 Score: 100.1 bits (248), Expect = 1.3e-21
Identity = 61/133 (45.86%), Postives = 82/133 (61.65%), Query Frame = 0


Query: 1   MAVIKCAIELKIGDVIESHGGS--MTLSQLSSSLKCCPSLLHRILRFLVHRGIFKEEKTT 60
           +A  KCAI+LKI + IE+H  S  +TL++LSS++   PS L RI+RFLVH+GIFKE  T 
Sbjct: 43  IAAAKCAIDLKIPEAIENHPSSQPVTLAELSSAVSASPSHLRRIMRFLVHQGIFKEIPTK 102

Query: 61  E----SYSQTPMSR--FLTTSGPNSLAPLHLLESSPVMVEPWHYLSSCLKA--KGS--QP 120
           +     Y  TP+SR   +T     SLAP  L E++P M+ PW  LSS + +   GS   P
Sbjct: 103 DGLATGYVNTPLSRRLMITRRDGKSLAPFVLFETTPEMLAPWLRLSSVVSSPVNGSTPPP 162

Query: 121 IEMAHGMDAWSYA 122
            +  HG D WS+A
Sbjct: 163 FDAVHGKDVWSFA 175

[Close]

BLAST of Lsi11G012320 vs. TAIR 10
Match: AT4G35150.1 (O-methyltransferase family protein )

HSP 1 Score: 70.5 bits (171), Expect = 1.1e-12
Identity = 38/74 (51.35%), Postives = 52/74 (70.27%), Query Frame = 0


Query: 1   MAVIKCAIELKIGDVIESHGGS--MTLSQLSSSLKCCPSLLHRILRFLVHRGIFKEEKTT 60
           +A  KCAI+LKI + IE+H  S  +TLS+LSS++   PS L RI+RFLVH+G+FKE  T 
Sbjct: 29  IAAAKCAIDLKIPEAIENHPSSQPVTLSELSSAVSASPSHLRRIMRFLVHQGLFKEVPTK 88

Query: 61  E----SYSQTPMSR 69
           +     Y+ TP+SR
Sbjct: 89  DGLATGYTNTPLSR 102

The following BLAST results are available for this feature:

BLAST of Lsi11G012320 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9T003	1.9e-20	45.86	Acetylserotonin O-methyltransferase OS=Arabidopsis thaliana OX=3702 GN=ASMT PE=1...	[more]
Q6WUC2	5.1e-18	41.09	(R,S)-reticuline 7-O-methyltransferase OS=Papaver somniferum OX=3469 GN=7OMT PE=...	[more]
B0ZB55	1.8e-15	35.88	Desmethylxanthohumol 6'-O-methyltransferase OS=Humulus lupulus OX=3486 GN=OMT1 P...	[more]
B0ZB56	2.2e-13	38.71	Xanthohumol 4-O-methyltransferase OS=Humulus lupulus OX=3486 GN=OMT2 PE=1 SV=1	[more]
Q6WUC1	1.0e-10	31.97	(RS)-norcoclaurine 6-O-methyltransferase OS=Papaver somniferum OX=3469 GN=6OMT P...	[more]

BLAST of Lsi11G012320 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K0Q2	6.8e-50	80.31	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G018720 PE=3 SV=1	[more]
A0A1S3BHY1	3.4e-49	77.95	(RS)-norcoclaurine 6-O-methyltransferase-like OS=Cucumis melo OX=3656 GN=LOC1034...	[more]
A0A5D3DI94	9.9e-49	77.17	(RS)-norcoclaurine 6-O-methyltransferase-like OS=Cucumis melo var. makuwa OX=119...	[more]
A0A6J1GLV0	2.6e-41	71.65	xanthohumol 4'-O-methyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1HVJ9	9.3e-39	68.50	xanthohumol 4'-O-methyltransferase-like isoform X2 OS=Cucurbita maxima OX=3661 G...	[more]

BLAST of Lsi11G012320 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004144871.1	1.4e-49	80.31	acetylserotonin O-methyltransferase [Cucumis sativus] >KGN43290.1 hypothetical p...	[more]
XP_008447905.1	7.0e-49	77.95	PREDICTED: (RS)-norcoclaurine 6-O-methyltransferase-like [Cucumis melo]	[more]
KAA0040348.1	2.0e-48	77.17	(RS)-norcoclaurine 6-O-methyltransferase-like [Cucumis melo var. makuwa] >TYK233...	[more]
XP_038888743.1	2.7e-48	80.00	acetylserotonin O-methyltransferase-like [Benincasa hispida]	[more]
KAG6572137.1	3.2e-41	71.65	Acetylserotonin O-methyltransferase, partial [Cucurbita argyrosperma subsp. soro...	[more]

BLAST of Lsi11G012320 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G35160.1	1.3e-21	45.86	O-methyltransferase family protein	[more]
AT4G35150.1	1.1e-12	51.35	O-methyltransferase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036388	Winged helix-like DNA-binding domain superfamily	GENE3D	1.10.10.10		coord: 1..70 e-value: 1.2E-21 score: 78.3
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 72..127 e-value: 1.0E-5 score: 26.6
None	No IPR available	PANTHER	PTHR11746:SF191	3'-HYDROXY-N-METHYL-(S)-COCLAURINE 4'-O-METHYLTRANSFERASE 2-LIKE	coord: 1..123
IPR012967	Plant methyltransferase dimerisation	PFAM	PF08100	Dimerisation	coord: 3..47 e-value: 2.1E-11 score: 43.7
IPR016461	O-methyltransferase COMT-type	PANTHER	PTHR11746	O-METHYLTRANSFERASE	coord: 1..123
IPR016461	O-methyltransferase COMT-type	PROSITE	PS51683	SAM_OMT_II	coord: 1..128 score: 21.881416
IPR036390	Winged helix DNA-binding domain superfamily	SUPERFAMILY	46785	"Winged helix" DNA-binding domain	coord: 2..74

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi11G012320.1	Lsi11G012320.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0019438	aromatic compound biosynthetic process
biological_process	GO:0032259	methylation
molecular_function	GO:0008171	O-methyltransferase activity
molecular_function	GO:0046983	protein dimerization activity
molecular_function	GO:0008757	S-adenosylmethionine-dependent methyltransferase activity
molecular_function	GO:0008168	methyltransferase activity