Lsi11G011030 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi11G011030
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial
Location	chr11: 19176932 .. 19182112 (+)
RNA-Seq Expression	Lsi11G011030
Synteny	Lsi11G011030

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GTGGGCTTGTATTGGGCTTGCTCAAATATCCACAACGAAGCCCAAGCCCAAGACGCAGCGTTTTATCGACCGGAAATCGGAACTGTCTCAGGGAATCCAGCGATCACAAGCTCAGTAGTTTCTGCAGAGAGAAGCATTTGGGAATCCCACCCTGTGAGTAGCGGTGAAAAACATTGGCTTACAACACAGCCACCATTTGCCATGGCTGCGATCTTAGCTCGGAAATCCCTTCTCGCTCTTCGTGCTCGTCACCTTGTAAGCAATCTTCACCTCTTTGCCCTAATTTTTCTACTTTGCCCCTCACACCCTTTTCCGGCGATGGACTTGTAAGACCCAATTGATTTAATACGGACGATGTTGAGCTTTTCTTCTGTTTTGGGATTTGTAGCGTTTGTAATTGAATCGAGGTTTTCTGGGTGACCATTTTTTCTTTTTAGTTTTGATATTCGAGTCGTAGAACAGATGGGGTATTCGTTTCCTTTTTATCGAATCATACAATTATGGTCATTGGGAATTGTTGTGTGTAATTTATCTGTTCCGTTGTGTCAACATTTTGATATGGAAATGGGGGAAATGGGACTATATGTTCTTTCTTTTCCCTAACTTGGAAGGTTTTGTTATTTTTAATTGTCCCATTTGGAGGGAGATTATGTTTAGAACGAGGAACTTGGAAGTACTAAATACTGGCAGAATAGGCAGTTGATGGTTAGGCTGTTTATATAATGCAATATCCTGAACTTTTCTGGAAAAATGTAGGCTATAATTTAGATATGTCGAAAAGATCTGTAGGTGGTTTCCGGAATTTTGCGGGAGATGTTCAAGACTTTGAATTATGCGTTGTATGTACTTTCCTTGAATTATATCCATGTGGTTCACGGTTAGAGAAAAAAAGAAGAGATTAGCATTATGGTGTTGTGCGGCTTAACCAATTACTTAGTCTGCAATCTTTTATTATTTATCTATTTCGACGTTGGGGTGGGAGGGTTCTGTTCATTGTTCACATTTGAAGGCCTTTTAGTGTTACTTTCTTTGATGAATTTTTCACTTTGATATTTGATGAAAAATATTATCTCCTAAGCTTTAGTTGTCATGGAGGTATTTACAATATTGATTGAAAATGTTTTTCTGGGAGGATCAATGGGTGGGAGATAGACCCCTCTCATCGGCGTTTCCTAGGTTATACTATTTGTCCTCTTTGAAAAATTGTTTCGTGTCGAACATTTTGGTTTGGTCAGGGAATTTTGTTTTTTTCTCCTTTGGTTTCCGCCCGGCGTTGTCCAATGGGGAAATGGCGGAGGTGGCCGCTCTTCTTTCCTTGATTGAGATTTTTGAATTTAGATTGGGGAGAAGGGATGTTCGTGTTTGGAGTCCTAATCCGGTGGAGGGCTTCTCTTGTAAGTCGTTCTTTAGGATTTTAGTGGATCCTTTTCTTGTTGTTGAGTCAGTCTTTGATGTTTTATGGAGGATTAAGATTCCAAAGAAAATTAGGTTCTTTGTCTGACAAGTTTTGCTTGGATGTGTGAATACGATGGATAGGTTTTTGAGAAAGATGCCATCATTAATGGGTTCTTTTTGTTGTATTCTTTGTCGGAAGGCCGGGGAAAACTTGGATCAATTTTGCTAGGAGTGTCAGCATGTGAGATTCGTTTGGATCTACTTCTTGCAGGAGTTTGAATTTGTTGCTTGTTTGCCAGAGGGACGTTCGGTTGATGATCAGAGAGTTCCTCCTCCATCCGCCTTTCAAAGAGAAAGGGCACTTACTTTGGTTTGCAGAGTGTGTGCTTTATTGTGAGCTCTGTGGGGAGAGAAACAATAGAGTGTTTAGGGTGTGGAGAGGGACCCTAGTGAGGTTTGGTCTTTGGTAAGCTTTTATGTTTCTCTTTGGGTTTTGATTTTGAAGACCTTTTGTACTTACACTCTAGGCAATATCTTACCTAGTTGGAAACCCTTTCTTCAGCGGGGATTTTTGCGGGCGTATTTTTTTTGTATGCCCTTGTATTCTTTCATTCTTTCTCAATGAAAACAGTTGTTTCTAAAGAAAAAAACAATATTGATTGAAAACCTGATGTGAATCATGCCACATAAATCATATTCCTTAATTTTGGTAGTTGTAACAACTTTTTGTTACCCTTTACTTTATCGCTCATGTGTATAGTCTACTGTTGTTAGGCCGTCTCTGGACAAGCACTGCAAGGTTCTCCTTTCCATGGTCTTCGGTCAAGTGCACACTTGTACAGTACCAAAAAAGGTTTCGTTTCTGGTTCTAACCATCCCAATAAATTTACTTGTATTACCATATTGGCATAATTAATCACTGCTGAAGCTGAACTTTGCAGTGTGTATATGATCCACAGGACATATACTTAATTCGTTTTATTTATGCCAGAATGGTGCTATGGAATTTTTTCTTTGCCTAAAATTCTTTTCCATCTCATTACTTCTGCAGAAGATGAAGAAAGAGAGCAGCTTGCCAAGGAGATCTCGAAGGATTGGAGTTCTAGTAATTTTTTATTTCCTTCCATAGTTTCTTCACTGTTTCCTTTTAAATCTTTTTCAGTTGTTTGTTCTATTATATTTCATGGTTCTTACAAAAATTAATTTCATGGCCTTTTTTAGTTTTTGAGCGTAGCATAAACACACTATTTCTCACTGAAATGGTCCGAGGTCTGATGCTGACTCTTAAATACTTCTTTGAGAAAAAAGTCACTGTAAGTGCCTTCCAACCAACCTTCTTTGGCACTAAGCTAAATTCTTGTTGATCCTGATTTATGATTATTGTACATGTACCATGCAGATCAATTATCCATTCGAGAAAGGCCCCCTAAGTCCTCGTTTTCGTGGTGAGCATGCGCTCCGACGTTATCCGACTGGAGAAGAACGATGCATTGCATGCAAACTTTGTGAAGCTGTAAGAATCTGGATCTATATTTTTAACTTTTCGTCCAAGTCATTTTGGTTTGGACATATAATTCTACAACGACAGGAGTTTGTATATTTCTTGCCATCATTGATTCTAGTTTAAATTATAAGTTTAATCCCCGAATTCTTTGGTTTGTGTGCATTTGGTCTTTGAATTTTCAAAAGTACCTAATAGGTCTCTGAAGTTTTAATTTTGTGTCAAATTGGTAAAGTGTCTGATAGATTCTTAGAATTTCAATTTTATATCCATTAAGTTTTTGAAATTTAAAAAGTATCTAATGGATCCTTAAACTTTCAATTTTGTATCCACGATCACCAAGACCCCGTTTGATAACCATTTGGTTTTTAGTTTTTGAAAATTAAGCCTACAAACACTCCTCCCACCCCTAAATGTCTTACTTTATTATCTACTTTGGACCCATGATTTAAAAAACCAAGCAAAAATTTGAAAACTAAAAATAGTAGTTTTTAAAAACTTGTTTTTGTTTTTGGAATTTGGCTAAGAATTCAACTCTTGTACTTAAGAAAGTTGCAAACTATTATAAGAAAATGTGAGAAAATAGGCTTAATTTTCAAAAATCAAAAACAAAAAACGAAATGGTTACCAAACGGGGTCTAACTTCTTCAATTTTGCATCTAATAGGTCCTTGACCTATTCAACATATATTATTTTTTAAGTTCACATATTTACTTAAAATAAAATTGAAATTTTAGGATTCCATTAGAGATAAAATTAAATTTTATATTTAATTGATCCATTATCAATTAATTTTTTCTAAATTGGATATGTAAGGGATCTATTAGATACAAAATTGTAAGTTCAGAAACATATTAGACACTTTTTGAAAATTTGGGGACCAAATAAACACAAATGTGATAGGTCAAAGACTAAACTTGTAATTTAATCTTGATTTTATAATCTCTCTTGTATTAAGCATCTTCAGTTGGAGGTGAAAATAATTGCTGTTCCTACTGGACTCATACTCTTAGATTTGTACCGTCCCTTAATGCATTGAAACGCATATTTTAATGTTATGTCGAAGTTGAGAAATCTTACTAGGATAGAATGTATCTAATCAGTGGTTTATTAGATCGCACTGCATCTGGTGATCAGGCATGGCTTTAATCCTTGACTGAAGAGTGTATTTGAGAGGGGTTCTCTAAGAGATGCAACTTTGAGAGCTATTTGTGCTCATATTTCTCAGTGGTTTATTAGATCGCACTGCATCTGCATAGTAGTCTTGCTCTGTAATCCTAACACGATGGATGATAACATATTCACATTTTAAATTTTATGCAGAACTTTGATAATGTTACATAAATCATAATATTTTAGCAGGCCATGCTTAAAATGTTTATGGTTTTGACCTTGAGATAAATTAAATACTTCAAACGATACATTTGAGCGTACACGTATCTGATCATTACTTTGAGTTGTGGCTTTGAACTTATATCTGTTATTCTATTTTGATTTTCGTTAGATTTGCCCTGCTCAAGCAATCACAATTGAAGCTGAGGAACGGGAAGATGGTAGCCGTAGGACCACAAGGTGATCACACCTCCACACCATCTCTTTATCGTTATATGTGTCCCTAAATAATCTTAATTTTCAGGTATGACATTGACATGACCAAATGCATCTACTGTGGGTTCTGTCAAGAGGCATGCCCTGTTGACGCCATTGTTGAAGGACCCAACTTTGAGTTTGCCACAGAGACGCACGAGGTTGATCTTACTATATTTTGAATGTTATGATAAGCTTCTAAACTTCCTGAAGAATGTTAGCCTCATAGTTTTGTGATGTTGTAGGAACTTCTCTATGACAAAGAAAAGTTGCTCGAGAACGGAGACCGCTGGGAGACCGAGATTGCAGAGAACCTCAGATCTGAAAGCCTTTATCGCTGATACATTTGCCCATGGTGCACTCTGGATTTGACTTTTCTTAGTGCGTTTATAATAAGATAAACCTGAGCATGCTGTTGAAAATTATTTTGTATTCTTGGTATACATGAGTTGAGAACATAATTCTTGCTGCTTATTACGGCCCAAGTTTGGGATTTTTATCAAAATCTTTTGTTTTTCTTCCCAATAAAAATGGGAATGTCATGGTTTCTCATGCAGTTGTAATGTAGGTAAAATATGAACAATATCATCAGTATAGTTTCAATGTTCTTCACGACTTCATTCTCTGGTATTGATTTTGAAAAAAAATGAAAATACCAGAAAATTTCATAAAATGGCCATTTGAATCTCTGTTTCTTG

mRNA sequence

GTGGGCTTGTATTGGGCTTGCTCAAATATCCACAACGAAGCCCAAGCCCAAGACGCAGCGTTTTATCGACCGGAAATCGGAACTGTCTCAGGGAATCCAGCGATCACAAGCTCAGTAGTTTCTGCAGAGAGAAGCATTTGGGAATCCCACCCTGTGAGTAGCGGTGAAAAACATTGGCTTACAACACAGCCACCATTTGCCATGGCTGCGATCTTAGCTCGGAAATCCCTTCTCGCTCTTCGTGCTCGTCACCTTGCCGTCTCTGGACAAGCACTGCAAGGTTCTCCTTTCCATGGTCTTCGGTCAAGTGCACACTTGTACAGTACCAAAAAAGGTTTCGTTTCTGAATGGTGCTATGGAATTTTTTCTTTGCCTAAAATTCTTTTCCATCTCATTACTTCTGCAGAAGATGAAGAAAGAGAGCAGCTTGCCAAGGAGATCTCGAAGGATTGGAGTTCTATTTTTGAGCGTAGCATAAACACACTATTTCTCACTGAAATGGTCCGAGGTCTGATGCTGACTCTTAAATACTTCTTTGAGAAAAAAGTCACTATCAATTATCCATTCGAGAAAGGCCCCCTAAGTCCTCGTTTTCGTGGTGAGCATGCGCTCCGACGTTATCCGACTGGAGAAGAACGATGCATTGCATGCAAACTTTGTGAAGCTATTTGCCCTGCTCAAGCAATCACAATTGAAGCTGAGGAACGGGAAGATGGTAGCCGTAGGACCACAAGGTATGACATTGACATGACCAAATGCATCTACTGTGGGTTCTGTCAAGAGGCATGCCCTGTTGACGCCATTGTTGAAGGACCCAACTTTGAGTTTGCCACAGAGACGCACGAGGAACTTCTCTATGACAAAGAAAAGTTGCTCGAGAACGGAGACCGCTGGGAGACCGAGATTGCAGAGAACCTCAGATCTGAAAGCCTTTATCGCTGATACATTTGCCCATGGTGCACTCTGGATTTGACTTTTCTTAGTGCGTTTATAATAAGATAAACCTGAGCATGCTGTTGAAAATTATTTTGTATTCTTGGTATACATGAGTTGAGAACATAATTCTTGCTGCTTATTACGGCCCAAGTTTGGGATTTTTATCAAAATCTTTTGTTTTTCTTCCCAATAAAAATGGGAATGTCATGGTTTCTCATGCAGTTGTAATGTAGGTAAAATATGAACAATATCATCAGTATAGTTTCAATGTTCTTCACGACTTCATTCTCTGGTATTGATTTTGAAAAAAAATGAAAATACCAGAAAATTTCATAAAATGGCCATTTGAATCTCTGTTTCTTG

Coding sequence (CDS)

GTGGGCTTGTATTGGGCTTGCTCAAATATCCACAACGAAGCCCAAGCCCAAGACGCAGCGTTTTATCGACCGGAAATCGGAACTGTCTCAGGGAATCCAGCGATCACAAGCTCAGTAGTTTCTGCAGAGAGAAGCATTTGGGAATCCCACCCTGTGAGTAGCGGTGAAAAACATTGGCTTACAACACAGCCACCATTTGCCATGGCTGCGATCTTAGCTCGGAAATCCCTTCTCGCTCTTCGTGCTCGTCACCTTGCCGTCTCTGGACAAGCACTGCAAGGTTCTCCTTTCCATGGTCTTCGGTCAAGTGCACACTTGTACAGTACCAAAAAAGGTTTCGTTTCTGAATGGTGCTATGGAATTTTTTCTTTGCCTAAAATTCTTTTCCATCTCATTACTTCTGCAGAAGATGAAGAAAGAGAGCAGCTTGCCAAGGAGATCTCGAAGGATTGGAGTTCTATTTTTGAGCGTAGCATAAACACACTATTTCTCACTGAAATGGTCCGAGGTCTGATGCTGACTCTTAAATACTTCTTTGAGAAAAAAGTCACTATCAATTATCCATTCGAGAAAGGCCCCCTAAGTCCTCGTTTTCGTGGTGAGCATGCGCTCCGACGTTATCCGACTGGAGAAGAACGATGCATTGCATGCAAACTTTGTGAAGCTATTTGCCCTGCTCAAGCAATCACAATTGAAGCTGAGGAACGGGAAGATGGTAGCCGTAGGACCACAAGGTATGACATTGACATGACCAAATGCATCTACTGTGGGTTCTGTCAAGAGGCATGCCCTGTTGACGCCATTGTTGAAGGACCCAACTTTGAGTTTGCCACAGAGACGCACGAGGAACTTCTCTATGACAAAGAAAAGTTGCTCGAGAACGGAGACCGCTGGGAGACCGAGATTGCAGAGAACCTCAGATCTGAAAGCCTTTATCGCTGA

Protein sequence

VGLYWACSNIHNEAQAQDAAFYRPEIGTVSGNPAITSSVVSAERSIWESHPVSSGEKHWLTTQPPFAMAAILARKSLLALRARHLAVSGQALQGSPFHGLRSSAHLYSTKKGFVSEWCYGIFSLPKILFHLITSAEDEEREQLAKEISKDWSSIFERSINTLFLTEMVRGLMLTLKYFFEKKVTINYPFEKGPLSPRFRGEHALRRYPTGEERCIACKLCEAICPAQAITIEAEEREDGSRRTTRYDIDMTKCIYCGFCQEACPVDAIVEGPNFEFATETHEELLYDKEKLLENGDRWETEIAENLRSESLYR

Homology

[Close]

BLAST of Lsi11G011030 vs. ExPASy Swiss-Prot
Match: Q9FX83 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 8-B, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g16700 PE=1 SV=1)

HSP 1 Score: 387.9 bits (995), Expect = 1.1e-106
Identity = 196/246 (79.67%), Postives = 207/246 (84.15%), Query Frame = 0


Query: 68  MAAILARKSLLALRARHLAVSGQALQGSPFHGLRSSAHLYSTKKGFVSEWCYGIFSLPKI 127
           MA++LAR+S  ALRARHLA SGQ LQGS   GL+S A  Y + K                
Sbjct: 1   MASLLARRSFSALRARHLAFSGQGLQGSHLCGLQSRAISYGSNK---------------- 60

Query: 128 LFHLITSAEDEEREQLAKEISKDWSSIFERSINTLFLTEMVRGLMLTLKYFFEKKVTINY 187
                   +DEE EQLAKEISKDWS++FERS+NTLFLTEMVRGL LTLKYFF+ KVTINY
Sbjct: 61  --------DDEEAEQLAKEISKDWSTVFERSMNTLFLTEMVRGLSLTLKYFFDPKVTINY 120

Query: 188 PFEKGPLSPRFRGEHALRRYPTGEERCIACKLCEAICPAQAITIEAEEREDGSRRTTRYD 247
           PFEKGPLSPRFRGEHALRRYPTGEERCIACKLCEA+CPAQAITIEAEEREDGSRRTTRYD
Sbjct: 121 PFEKGPLSPRFRGEHALRRYPTGEERCIACKLCEAVCPAQAITIEAEEREDGSRRTTRYD 180

Query: 248 IDMTKCIYCGFCQEACPVDAIVEGPNFEFATETHEELLYDKEKLLENGDRWETEIAENLR 307
           IDMTKCIYCGFCQEACPVDAIVEGPNFEFATETHEELLYDKEKLLENGDRWETEIAENLR
Sbjct: 181 IDMTKCIYCGFCQEACPVDAIVEGPNFEFATETHEELLYDKEKLLENGDRWETEIAENLR 222

Query: 308 SESLYR 314
           SESLYR
Sbjct: 241 SESLYR 222

[Close]

BLAST of Lsi11G011030 vs. ExPASy Swiss-Prot
Match: Q42599 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 8-A, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g79010 PE=1 SV=1)

HSP 1 Score: 384.0 bits (985), Expect = 1.6e-105
Identity = 194/246 (78.86%), Postives = 206/246 (83.74%), Query Frame = 0


Query: 68  MAAILARKSLLALRARHLAVSGQALQGSPFHGLRSSAHLYSTKKGFVSEWCYGIFSLPKI 127
           MA+ILAR+SL  LRARHL +SGQALQGS    L+S    Y + K                
Sbjct: 1   MASILARRSLNTLRARHLVLSGQALQGSHLSRLQSRGISYGSNK---------------- 60

Query: 128 LFHLITSAEDEEREQLAKEISKDWSSIFERSINTLFLTEMVRGLMLTLKYFFEKKVTINY 187
                   +DEE EQL+KEISKDW+++FERSINTLFLTEMVRGL LTLKYFF+ KVTINY
Sbjct: 61  --------DDEEAEQLSKEISKDWNTVFERSINTLFLTEMVRGLSLTLKYFFDPKVTINY 120

Query: 188 PFEKGPLSPRFRGEHALRRYPTGEERCIACKLCEAICPAQAITIEAEEREDGSRRTTRYD 247
           PFEKGPLSPRFRGEHALRRYPTGEERCIACKLCEA+CPAQAITIEAEEREDGSRRTTRYD
Sbjct: 121 PFEKGPLSPRFRGEHALRRYPTGEERCIACKLCEAVCPAQAITIEAEEREDGSRRTTRYD 180

Query: 248 IDMTKCIYCGFCQEACPVDAIVEGPNFEFATETHEELLYDKEKLLENGDRWETEIAENLR 307
           IDMTKCIYCGFCQEACPVDAIVEGPNFEFATETHEELLYDKEKLLENGDRWETEIAENLR
Sbjct: 181 IDMTKCIYCGFCQEACPVDAIVEGPNFEFATETHEELLYDKEKLLENGDRWETEIAENLR 222

Query: 308 SESLYR 314
           SESLYR
Sbjct: 241 SESLYR 222

[Close]

BLAST of Lsi11G011030 vs. ExPASy Swiss-Prot
Match: P80269 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial OS=Solanum tuberosum OX=4113 PE=1 SV=2)

HSP 1 Score: 382.5 bits (981), Expect = 4.6e-105
Identity = 194/247 (78.54%), Postives = 208/247 (84.21%), Query Frame = 0


Query: 68  MAAILARKSLLALRARHLAVSGQALQG-SPFHGLRSSAHLYSTKKGFVSEWCYGIFSLPK 127
           MAAILARKSL ALR+R L ++G  ++G + ++        ++TK  F             
Sbjct: 1   MAAILARKSLSALRSRQLVLAGHTIEGTNGYNRTLLGTRSFATKHSF------------- 60

Query: 128 ILFHLITSAEDEEREQLAKEISKDWSSIFERSINTLFLTEMVRGLMLTLKYFFEKKVTIN 187
                 T  +DEEREQLAKE+SKDW+S+FERSINTLFLTEMVRGLMLTLKYFFEKKVTIN
Sbjct: 61  -----STDKDDEEREQLAKELSKDWNSVFERSINTLFLTEMVRGLMLTLKYFFEKKVTIN 120

Query: 188 YPFEKGPLSPRFRGEHALRRYPTGEERCIACKLCEAICPAQAITIEAEEREDGSRRTTRY 247
           YPFEKGPLSPRFRGEHALRRY TGEERCIACKLCEAICPAQAITIEAEEREDGSRRTTRY
Sbjct: 121 YPFEKGPLSPRFRGEHALRRYATGEERCIACKLCEAICPAQAITIEAEEREDGSRRTTRY 180

Query: 248 DIDMTKCIYCGFCQEACPVDAIVEGPNFEFATETHEELLYDKEKLLENGDRWETEIAENL 307
           DIDMTKCIYCGFCQEACPVDAIVEGPNFEFATETHEELLYDKEKLLENGDRWETEIAENL
Sbjct: 181 DIDMTKCIYCGFCQEACPVDAIVEGPNFEFATETHEELLYDKEKLLENGDRWETEIAENL 229

Query: 308 RSESLYR 314
           RSESLYR
Sbjct: 241 RSESLYR 229

[Close]

BLAST of Lsi11G011030 vs. ExPASy Swiss-Prot
Match: O24143 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 375.6 bits (963), Expect = 5.6e-103
Identity = 192/248 (77.42%), Postives = 204/248 (82.26%), Query Frame = 0


Query: 68  MAAILARKSLLALRARHLAVSGQALQ--GSPFHGLRSSAHLYSTKKGFVSEWCYGIFSLP 127
           MAAILARKSL ALR+R L ++GQA Q   +  +G       ++T   F            
Sbjct: 1   MAAILARKSLSALRSRQLVLAGQAWQQGANTSNGTLLGTRTFATNDSF------------ 60

Query: 128 KILFHLITSAEDEEREQLAKEISKDWSSIFERSINTLFLTEMVRGLMLTLKYFFEKKVTI 187
                  T  +DEEREQLAKE+SKDW+S+FE+ INTLFLTEMVRGLMLTLKYFFEKKVTI
Sbjct: 61  ------STDKDDEEREQLAKELSKDWNSVFEQQINTLFLTEMVRGLMLTLKYFFEKKVTI 120

Query: 188 NYPFEKGPLSPRFRGEHALRRYPTGEERCIACKLCEAICPAQAITIEAEEREDGSRRTTR 247
           NYPFEKGPLSPRFRGE  LRRYPTGEERCIACKLCEAICPAQAITIEAE REDGSRRTTR
Sbjct: 121 NYPFEKGPLSPRFRGEQPLRRYPTGEERCIACKLCEAICPAQAITIEAEAREDGSRRTTR 180

Query: 248 YDIDMTKCIYCGFCQEACPVDAIVEGPNFEFATETHEELLYDKEKLLENGDRWETEIAEN 307
           YDIDMTKCIYCGFCQEACPVDAIVEGPNFEFATETHEELLYDKEKLLENGDRWETEIAEN
Sbjct: 181 YDIDMTKCIYCGFCQEACPVDAIVEGPNFEFATETHEELLYDKEKLLENGDRWETEIAEN 230

Query: 308 LRSESLYR 314
           LRSESLYR
Sbjct: 241 LRSESLYR 230

[Close]

BLAST of Lsi11G011030 vs. ExPASy Swiss-Prot
Match: O21233 (NADH-ubiquinone oxidoreductase subunit 8 OS=Reclinomonas americana OX=48483 GN=NAD8 PE=3 SV=1)

HSP 1 Score: 295.4 bits (755), Expect = 7.4e-79
Identity = 138/161 (85.71%), Postives = 149/161 (92.55%), Query Frame = 0


Query: 153 SIFERSINTLFLTEMVRGLMLTLKYFFEKKVTINYPFEKGPLSPRFRGEHALRRYPTGEE 212
           +I  ++  TLFLTE+V+G+ LTL YFF KKVT+NYPFEKGPLSPRFRGEHALRRY TGEE
Sbjct: 2   TIINKTAQTLFLTELVKGMSLTLDYFFRKKVTLNYPFEKGPLSPRFRGEHALRRYQTGEE 61

Query: 213 RCIACKLCEAICPAQAITIEAEEREDGSRRTTRYDIDMTKCIYCGFCQEACPVDAIVEGP 272
           RCIACKLCEAICPAQAITIE+E R DGSRRTTRYDIDMTKCIYCGFCQEACPVDAIVEGP
Sbjct: 62  RCIACKLCEAICPAQAITIESEPRIDGSRRTTRYDIDMTKCIYCGFCQEACPVDAIVEGP 121

Query: 273 NFEFATETHEELLYDKEKLLENGDRWETEIAENLRSESLYR 314
           NFEFATETHEELLYDKEKLL+NGDRWETEIA NL +E+LYR
Sbjct: 122 NFEFATETHEELLYDKEKLLQNGDRWETEIAANLANEALYR 162

[Close]

BLAST of Lsi11G011030 vs. ExPASy TrEMBL
Match: A0A6J1C2E8 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111007833 PE=3 SV=1)

HSP 1 Score: 429.9 bits (1104), Expect = 9.3e-117
Identity = 219/246 (89.02%), Postives = 221/246 (89.84%), Query Frame = 0


Query: 68  MAAILARKSLLALRARHLAVSGQALQGSPFHGLRSSAHLYSTKKGFVSEWCYGIFSLPKI 127
           MAAILARKSLLALRARHLAVSGQALQGSPFHGLRSSAHL+STKK                
Sbjct: 1   MAAILARKSLLALRARHLAVSGQALQGSPFHGLRSSAHLFSTKK---------------- 60

Query: 128 LFHLITSAEDEEREQLAKEISKDWSSIFERSINTLFLTEMVRGLMLTLKYFFEKKVTINY 187
                   +DEEREQLAKEISKDWSSIFERSINTLFLTEMVRGLMLTLKYFFEKKVTINY
Sbjct: 61  --------DDEEREQLAKEISKDWSSIFERSINTLFLTEMVRGLMLTLKYFFEKKVTINY 120

Query: 188 PFEKGPLSPRFRGEHALRRYPTGEERCIACKLCEAICPAQAITIEAEEREDGSRRTTRYD 247
           PFEKGPLSPRFRGEHALRRY TGEERCIACKLCEAICPAQAITIEAEEREDGSRRTTRYD
Sbjct: 121 PFEKGPLSPRFRGEHALRRYATGEERCIACKLCEAICPAQAITIEAEEREDGSRRTTRYD 180

Query: 248 IDMTKCIYCGFCQEACPVDAIVEGPNFEFATETHEELLYDKEKLLENGDRWETEIAENLR 307
           IDMTKCIYCGFCQEACPVDAIVEGPNFEFATETHEELLYDKEKLLENGDRWETEIAENLR
Sbjct: 181 IDMTKCIYCGFCQEACPVDAIVEGPNFEFATETHEELLYDKEKLLENGDRWETEIAENLR 222

Query: 308 SESLYR 314
           SESLYR
Sbjct: 241 SESLYR 222

[Close]

BLAST of Lsi11G011030 vs. ExPASy TrEMBL
Match: A0A6J1I8H7 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111470147 PE=3 SV=1)

HSP 1 Score: 424.1 bits (1089), Expect = 5.1e-115
Identity = 215/246 (87.40%), Postives = 219/246 (89.02%), Query Frame = 0


Query: 68  MAAILARKSLLALRARHLAVSGQALQGSPFHGLRSSAHLYSTKKGFVSEWCYGIFSLPKI 127
           MAAILARKSLLALRAR  A+SGQALQGSPFHGLRSSAHLYSTK+                
Sbjct: 1   MAAILARKSLLALRARQFAISGQALQGSPFHGLRSSAHLYSTKR---------------- 60

Query: 128 LFHLITSAEDEEREQLAKEISKDWSSIFERSINTLFLTEMVRGLMLTLKYFFEKKVTINY 187
                   EDEEREQLAKE+SKDW+SIFERSINTLFLTEMVRGLMLTLKYFFEKKVTINY
Sbjct: 61  --------EDEEREQLAKEMSKDWNSIFERSINTLFLTEMVRGLMLTLKYFFEKKVTINY 120

Query: 188 PFEKGPLSPRFRGEHALRRYPTGEERCIACKLCEAICPAQAITIEAEEREDGSRRTTRYD 247
           PFEKGPLSPRFRGEHALRRY TGEERCIACKLCEAICPAQAITIEAEEREDGSRRTTRYD
Sbjct: 121 PFEKGPLSPRFRGEHALRRYATGEERCIACKLCEAICPAQAITIEAEEREDGSRRTTRYD 180

Query: 248 IDMTKCIYCGFCQEACPVDAIVEGPNFEFATETHEELLYDKEKLLENGDRWETEIAENLR 307
           IDMTKCIYCGFCQEACPVDAIVEGPNFEFATETHEELLYDKEKLLENGDRWETEIAENLR
Sbjct: 181 IDMTKCIYCGFCQEACPVDAIVEGPNFEFATETHEELLYDKEKLLENGDRWETEIAENLR 222

Query: 308 SESLYR 314
           SESLYR
Sbjct: 241 SESLYR 222

[Close]

BLAST of Lsi11G011030 vs. ExPASy TrEMBL
Match: A0A6J1F0I7 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111441225 PE=3 SV=1)

HSP 1 Score: 424.1 bits (1089), Expect = 5.1e-115
Identity = 215/246 (87.40%), Postives = 219/246 (89.02%), Query Frame = 0


Query: 68  MAAILARKSLLALRARHLAVSGQALQGSPFHGLRSSAHLYSTKKGFVSEWCYGIFSLPKI 127
           MAAILARKSLLALRAR  A+SGQALQGSPFHGLRSSAHLYSTK+                
Sbjct: 1   MAAILARKSLLALRARQFAISGQALQGSPFHGLRSSAHLYSTKR---------------- 60

Query: 128 LFHLITSAEDEEREQLAKEISKDWSSIFERSINTLFLTEMVRGLMLTLKYFFEKKVTINY 187
                   EDEEREQLAKE+SKDW+SIFERSINTLFLTEMVRGLMLTLKYFFEKKVTINY
Sbjct: 61  --------EDEEREQLAKEMSKDWNSIFERSINTLFLTEMVRGLMLTLKYFFEKKVTINY 120

Query: 188 PFEKGPLSPRFRGEHALRRYPTGEERCIACKLCEAICPAQAITIEAEEREDGSRRTTRYD 247
           PFEKGPLSPRFRGEHALRRY TGEERCIACKLCEAICPAQAITIEAEEREDGSRRTTRYD
Sbjct: 121 PFEKGPLSPRFRGEHALRRYATGEERCIACKLCEAICPAQAITIEAEEREDGSRRTTRYD 180

Query: 248 IDMTKCIYCGFCQEACPVDAIVEGPNFEFATETHEELLYDKEKLLENGDRWETEIAENLR 307
           IDMTKCIYCGFCQEACPVDAIVEGPNFEFATETHEELLYDKEKLLENGDRWETEIAENLR
Sbjct: 181 IDMTKCIYCGFCQEACPVDAIVEGPNFEFATETHEELLYDKEKLLENGDRWETEIAENLR 222

Query: 308 SESLYR 314
           SESLYR
Sbjct: 241 SESLYR 222

[Close]

BLAST of Lsi11G011030 vs. ExPASy TrEMBL
Match: A0A6J1GJX8 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial-like OS=Cucurbita moschata OX=3662 GN=LOC111455001 PE=3 SV=1)

HSP 1 Score: 420.6 bits (1080), Expect = 5.6e-114
Identity = 215/246 (87.40%), Postives = 218/246 (88.62%), Query Frame = 0


Query: 68  MAAILARKSLLALRARHLAVSGQALQGSPFHGLRSSAHLYSTKKGFVSEWCYGIFSLPKI 127
           MAAILARKSLLALRAR LA+SGQALQGS FH LRSSAHLYSTK+                
Sbjct: 1   MAAILARKSLLALRARQLAISGQALQGSRFHDLRSSAHLYSTKR---------------- 60

Query: 128 LFHLITSAEDEEREQLAKEISKDWSSIFERSINTLFLTEMVRGLMLTLKYFFEKKVTINY 187
                   EDEEREQLAKE+SKDWSSIFERSINTLFLTEMVRGLMLTLKYFFEKKVTINY
Sbjct: 61  --------EDEEREQLAKEMSKDWSSIFERSINTLFLTEMVRGLMLTLKYFFEKKVTINY 120

Query: 188 PFEKGPLSPRFRGEHALRRYPTGEERCIACKLCEAICPAQAITIEAEEREDGSRRTTRYD 247
           PFEKGPLSPRFRGEHALRRY TGEERCIACKLCEAICPAQAITIEAEEREDGSRRTTRYD
Sbjct: 121 PFEKGPLSPRFRGEHALRRYTTGEERCIACKLCEAICPAQAITIEAEEREDGSRRTTRYD 180

Query: 248 IDMTKCIYCGFCQEACPVDAIVEGPNFEFATETHEELLYDKEKLLENGDRWETEIAENLR 307
           IDMTKCIYCGFCQEACPVDAIVEGPNFEFATETHEELLYDKEKLLENGDRWETEIAENLR
Sbjct: 181 IDMTKCIYCGFCQEACPVDAIVEGPNFEFATETHEELLYDKEKLLENGDRWETEIAENLR 222

Query: 308 SESLYR 314
           SESLYR
Sbjct: 241 SESLYR 222

[Close]

BLAST of Lsi11G011030 vs. ExPASy TrEMBL
Match: A0A0A0K5Z9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G268510 PE=3 SV=1)

HSP 1 Score: 418.3 bits (1074), Expect = 2.8e-113
Identity = 215/246 (87.40%), Postives = 218/246 (88.62%), Query Frame = 0


Query: 68  MAAILARKSLLALRARHLAVSGQALQGSPFHGLRSSAHLYSTKKGFVSEWCYGIFSLPKI 127
           MAAILARKSLLALRAR LAVSGQALQGSPFHGL+S+ HLYST K                
Sbjct: 1   MAAILARKSLLALRARQLAVSGQALQGSPFHGLQST-HLYSTSK---------------- 60

Query: 128 LFHLITSAEDEEREQLAKEISKDWSSIFERSINTLFLTEMVRGLMLTLKYFFEKKVTINY 187
                   +DEEREQLAKEISKDWSSIFERSINTLFLTEMVRGL LTLKYFFEKKVTINY
Sbjct: 61  --------DDEEREQLAKEISKDWSSIFERSINTLFLTEMVRGLSLTLKYFFEKKVTINY 120

Query: 188 PFEKGPLSPRFRGEHALRRYPTGEERCIACKLCEAICPAQAITIEAEEREDGSRRTTRYD 247
           PFEKGPLSPRFRGEHALRRYPTGEERCIACKLCEAICPAQAITIEAEEREDGSRRTTRYD
Sbjct: 121 PFEKGPLSPRFRGEHALRRYPTGEERCIACKLCEAICPAQAITIEAEEREDGSRRTTRYD 180

Query: 248 IDMTKCIYCGFCQEACPVDAIVEGPNFEFATETHEELLYDKEKLLENGDRWETEIAENLR 307
           IDMTKCIYCGFCQEACPVDAIVEGPNFEFATETHEELLYDKEKLLENGDRWETEIAENLR
Sbjct: 181 IDMTKCIYCGFCQEACPVDAIVEGPNFEFATETHEELLYDKEKLLENGDRWETEIAENLR 221

Query: 308 SESLYR 314
           SESLYR
Sbjct: 241 SESLYR 221

[Close]

BLAST of Lsi11G011030 vs. NCBI nr
Match: XP_038887775.1 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 8-B, mitochondrial [Benincasa hispida])

HSP 1 Score: 432.6 bits (1111), Expect = 3.0e-117
Identity = 221/246 (89.84%), Postives = 221/246 (89.84%), Query Frame = 0


Query: 68  MAAILARKSLLALRARHLAVSGQALQGSPFHGLRSSAHLYSTKKGFVSEWCYGIFSLPKI 127
           MAAILARKSLLALRAR LAVSGQALQGSPFHGLRSSAHLYSTKK                
Sbjct: 1   MAAILARKSLLALRARQLAVSGQALQGSPFHGLRSSAHLYSTKK---------------- 60

Query: 128 LFHLITSAEDEEREQLAKEISKDWSSIFERSINTLFLTEMVRGLMLTLKYFFEKKVTINY 187
                   EDEEREQLAKEISKDWSSIFERSINTLFLTEMVRGLMLTLKYFFEKKVTINY
Sbjct: 61  --------EDEEREQLAKEISKDWSSIFERSINTLFLTEMVRGLMLTLKYFFEKKVTINY 120

Query: 188 PFEKGPLSPRFRGEHALRRYPTGEERCIACKLCEAICPAQAITIEAEEREDGSRRTTRYD 247
           PFEKGPLSPRFRGEHALRRYPTGEERCIACKLCEAICPAQAITIEAEEREDGSRRTTRYD
Sbjct: 121 PFEKGPLSPRFRGEHALRRYPTGEERCIACKLCEAICPAQAITIEAEEREDGSRRTTRYD 180

Query: 248 IDMTKCIYCGFCQEACPVDAIVEGPNFEFATETHEELLYDKEKLLENGDRWETEIAENLR 307
           IDMTKCIYCGFCQEACPVDAIVEGPNFEFATETHEELLYDKEKLLENGDRWETEIAENLR
Sbjct: 181 IDMTKCIYCGFCQEACPVDAIVEGPNFEFATETHEELLYDKEKLLENGDRWETEIAENLR 222

Query: 308 SESLYR 314
           SESLYR
Sbjct: 241 SESLYR 222

[Close]

BLAST of Lsi11G011030 vs. NCBI nr
Match: XP_022136046.1 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial [Momordica charantia])

HSP 1 Score: 429.9 bits (1104), Expect = 1.9e-116
Identity = 219/246 (89.02%), Postives = 221/246 (89.84%), Query Frame = 0


Query: 68  MAAILARKSLLALRARHLAVSGQALQGSPFHGLRSSAHLYSTKKGFVSEWCYGIFSLPKI 127
           MAAILARKSLLALRARHLAVSGQALQGSPFHGLRSSAHL+STKK                
Sbjct: 1   MAAILARKSLLALRARHLAVSGQALQGSPFHGLRSSAHLFSTKK---------------- 60

Query: 128 LFHLITSAEDEEREQLAKEISKDWSSIFERSINTLFLTEMVRGLMLTLKYFFEKKVTINY 187
                   +DEEREQLAKEISKDWSSIFERSINTLFLTEMVRGLMLTLKYFFEKKVTINY
Sbjct: 61  --------DDEEREQLAKEISKDWSSIFERSINTLFLTEMVRGLMLTLKYFFEKKVTINY 120

Query: 188 PFEKGPLSPRFRGEHALRRYPTGEERCIACKLCEAICPAQAITIEAEEREDGSRRTTRYD 247
           PFEKGPLSPRFRGEHALRRY TGEERCIACKLCEAICPAQAITIEAEEREDGSRRTTRYD
Sbjct: 121 PFEKGPLSPRFRGEHALRRYATGEERCIACKLCEAICPAQAITIEAEEREDGSRRTTRYD 180

Query: 248 IDMTKCIYCGFCQEACPVDAIVEGPNFEFATETHEELLYDKEKLLENGDRWETEIAENLR 307
           IDMTKCIYCGFCQEACPVDAIVEGPNFEFATETHEELLYDKEKLLENGDRWETEIAENLR
Sbjct: 181 IDMTKCIYCGFCQEACPVDAIVEGPNFEFATETHEELLYDKEKLLENGDRWETEIAENLR 222

Query: 308 SESLYR 314
           SESLYR
Sbjct: 241 SESLYR 222

[Close]

BLAST of Lsi11G011030 vs. NCBI nr
Match: KAG7011706.1 (hypothetical protein SDJN02_26612 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 428.3 bits (1100), Expect = 5.6e-116
Identity = 218/246 (88.62%), Postives = 221/246 (89.84%), Query Frame = 0


Query: 68  MAAILARKSLLALRARHLAVSGQALQGSPFHGLRSSAHLYSTKKGFVSEWCYGIFSLPKI 127
           M AILARKSLLALRAR LA+SGQALQGS FH LRSSAHLYSTK+GFVS            
Sbjct: 1   MTAILARKSLLALRARQLAISGQALQGSRFHDLRSSAHLYSTKRGFVS------------ 60

Query: 128 LFHLITSAEDEEREQLAKEISKDWSSIFERSINTLFLTEMVRGLMLTLKYFFEKKVTINY 187
                   EDEEREQLAKE+SKDWSSIFERSINTLFLTEMVRGLMLTLKYFFEKKVTINY
Sbjct: 61  --------EDEEREQLAKEMSKDWSSIFERSINTLFLTEMVRGLMLTLKYFFEKKVTINY 120

Query: 188 PFEKGPLSPRFRGEHALRRYPTGEERCIACKLCEAICPAQAITIEAEEREDGSRRTTRYD 247
           PFEKGPLSPRFRGEHALRRY TGEERCIACKLCEAICPAQAITIEAEEREDGSRRTTRYD
Sbjct: 121 PFEKGPLSPRFRGEHALRRYTTGEERCIACKLCEAICPAQAITIEAEEREDGSRRTTRYD 180

Query: 248 IDMTKCIYCGFCQEACPVDAIVEGPNFEFATETHEELLYDKEKLLENGDRWETEIAENLR 307
           IDMTKCIYCGFCQEACPVDAIVEGPNFEFATETHEELLYDKEKLLENGDRWETEIAENLR
Sbjct: 181 IDMTKCIYCGFCQEACPVDAIVEGPNFEFATETHEELLYDKEKLLENGDRWETEIAENLR 226

Query: 308 SESLYR 314
           SESLYR
Sbjct: 241 SESLYR 226

[Close]

BLAST of Lsi11G011030 vs. NCBI nr
Match: XP_022933976.1 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial [Cucurbita moschata] >XP_022971419.1 NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial [Cucurbita maxima] >XP_023529277.1 NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial [Cucurbita pepo subsp. pepo] >KAG6588437.1 hypothetical protein SDJN03_17002, partial [Cucurbita argyrosperma subsp. sororia] >KAG7022285.1 hypothetical protein SDJN02_16016 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 424.1 bits (1089), Expect = 1.1e-114
Identity = 215/246 (87.40%), Postives = 219/246 (89.02%), Query Frame = 0


Query: 68  MAAILARKSLLALRARHLAVSGQALQGSPFHGLRSSAHLYSTKKGFVSEWCYGIFSLPKI 127
           MAAILARKSLLALRAR  A+SGQALQGSPFHGLRSSAHLYSTK+                
Sbjct: 1   MAAILARKSLLALRARQFAISGQALQGSPFHGLRSSAHLYSTKR---------------- 60

Query: 128 LFHLITSAEDEEREQLAKEISKDWSSIFERSINTLFLTEMVRGLMLTLKYFFEKKVTINY 187
                   EDEEREQLAKE+SKDW+SIFERSINTLFLTEMVRGLMLTLKYFFEKKVTINY
Sbjct: 61  --------EDEEREQLAKEMSKDWNSIFERSINTLFLTEMVRGLMLTLKYFFEKKVTINY 120

Query: 188 PFEKGPLSPRFRGEHALRRYPTGEERCIACKLCEAICPAQAITIEAEEREDGSRRTTRYD 247
           PFEKGPLSPRFRGEHALRRY TGEERCIACKLCEAICPAQAITIEAEEREDGSRRTTRYD
Sbjct: 121 PFEKGPLSPRFRGEHALRRYATGEERCIACKLCEAICPAQAITIEAEEREDGSRRTTRYD 180

Query: 248 IDMTKCIYCGFCQEACPVDAIVEGPNFEFATETHEELLYDKEKLLENGDRWETEIAENLR 307
           IDMTKCIYCGFCQEACPVDAIVEGPNFEFATETHEELLYDKEKLLENGDRWETEIAENLR
Sbjct: 181 IDMTKCIYCGFCQEACPVDAIVEGPNFEFATETHEELLYDKEKLLENGDRWETEIAENLR 222

Query: 308 SESLYR 314
           SESLYR
Sbjct: 241 SESLYR 222

[Close]

BLAST of Lsi11G011030 vs. NCBI nr
Match: XP_022952272.1 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial-like [Cucurbita moschata])

HSP 1 Score: 420.6 bits (1080), Expect = 1.2e-113
Identity = 215/246 (87.40%), Postives = 218/246 (88.62%), Query Frame = 0


Query: 68  MAAILARKSLLALRARHLAVSGQALQGSPFHGLRSSAHLYSTKKGFVSEWCYGIFSLPKI 127
           MAAILARKSLLALRAR LA+SGQALQGS FH LRSSAHLYSTK+                
Sbjct: 1   MAAILARKSLLALRARQLAISGQALQGSRFHDLRSSAHLYSTKR---------------- 60

Query: 128 LFHLITSAEDEEREQLAKEISKDWSSIFERSINTLFLTEMVRGLMLTLKYFFEKKVTINY 187
                   EDEEREQLAKE+SKDWSSIFERSINTLFLTEMVRGLMLTLKYFFEKKVTINY
Sbjct: 61  --------EDEEREQLAKEMSKDWSSIFERSINTLFLTEMVRGLMLTLKYFFEKKVTINY 120

Query: 188 PFEKGPLSPRFRGEHALRRYPTGEERCIACKLCEAICPAQAITIEAEEREDGSRRTTRYD 247
           PFEKGPLSPRFRGEHALRRY TGEERCIACKLCEAICPAQAITIEAEEREDGSRRTTRYD
Sbjct: 121 PFEKGPLSPRFRGEHALRRYTTGEERCIACKLCEAICPAQAITIEAEEREDGSRRTTRYD 180

Query: 248 IDMTKCIYCGFCQEACPVDAIVEGPNFEFATETHEELLYDKEKLLENGDRWETEIAENLR 307
           IDMTKCIYCGFCQEACPVDAIVEGPNFEFATETHEELLYDKEKLLENGDRWETEIAENLR
Sbjct: 181 IDMTKCIYCGFCQEACPVDAIVEGPNFEFATETHEELLYDKEKLLENGDRWETEIAENLR 222

Query: 308 SESLYR 314
           SESLYR
Sbjct: 241 SESLYR 222

[Close]

BLAST of Lsi11G011030 vs. TAIR 10
Match: AT1G16700.1 (Alpha-helical ferredoxin )

HSP 1 Score: 387.9 bits (995), Expect = 7.8e-108
Identity = 196/246 (79.67%), Postives = 207/246 (84.15%), Query Frame = 0


Query: 68  MAAILARKSLLALRARHLAVSGQALQGSPFHGLRSSAHLYSTKKGFVSEWCYGIFSLPKI 127
           MA++LAR+S  ALRARHLA SGQ LQGS   GL+S A  Y + K                
Sbjct: 1   MASLLARRSFSALRARHLAFSGQGLQGSHLCGLQSRAISYGSNK---------------- 60

Query: 128 LFHLITSAEDEEREQLAKEISKDWSSIFERSINTLFLTEMVRGLMLTLKYFFEKKVTINY 187
                   +DEE EQLAKEISKDWS++FERS+NTLFLTEMVRGL LTLKYFF+ KVTINY
Sbjct: 61  --------DDEEAEQLAKEISKDWSTVFERSMNTLFLTEMVRGLSLTLKYFFDPKVTINY 120

Query: 188 PFEKGPLSPRFRGEHALRRYPTGEERCIACKLCEAICPAQAITIEAEEREDGSRRTTRYD 247
           PFEKGPLSPRFRGEHALRRYPTGEERCIACKLCEA+CPAQAITIEAEEREDGSRRTTRYD
Sbjct: 121 PFEKGPLSPRFRGEHALRRYPTGEERCIACKLCEAVCPAQAITIEAEEREDGSRRTTRYD 180

Query: 248 IDMTKCIYCGFCQEACPVDAIVEGPNFEFATETHEELLYDKEKLLENGDRWETEIAENLR 307
           IDMTKCIYCGFCQEACPVDAIVEGPNFEFATETHEELLYDKEKLLENGDRWETEIAENLR
Sbjct: 181 IDMTKCIYCGFCQEACPVDAIVEGPNFEFATETHEELLYDKEKLLENGDRWETEIAENLR 222

Query: 308 SESLYR 314
           SESLYR
Sbjct: 241 SESLYR 222

[Close]

BLAST of Lsi11G011030 vs. TAIR 10
Match: AT1G79010.1 (Alpha-helical ferredoxin )

HSP 1 Score: 384.0 bits (985), Expect = 1.1e-106
Identity = 194/246 (78.86%), Postives = 206/246 (83.74%), Query Frame = 0


Query: 68  MAAILARKSLLALRARHLAVSGQALQGSPFHGLRSSAHLYSTKKGFVSEWCYGIFSLPKI 127
           MA+ILAR+SL  LRARHL +SGQALQGS    L+S    Y + K                
Sbjct: 1   MASILARRSLNTLRARHLVLSGQALQGSHLSRLQSRGISYGSNK---------------- 60

Query: 128 LFHLITSAEDEEREQLAKEISKDWSSIFERSINTLFLTEMVRGLMLTLKYFFEKKVTINY 187
                   +DEE EQL+KEISKDW+++FERSINTLFLTEMVRGL LTLKYFF+ KVTINY
Sbjct: 61  --------DDEEAEQLSKEISKDWNTVFERSINTLFLTEMVRGLSLTLKYFFDPKVTINY 120

Query: 188 PFEKGPLSPRFRGEHALRRYPTGEERCIACKLCEAICPAQAITIEAEEREDGSRRTTRYD 247
           PFEKGPLSPRFRGEHALRRYPTGEERCIACKLCEA+CPAQAITIEAEEREDGSRRTTRYD
Sbjct: 121 PFEKGPLSPRFRGEHALRRYPTGEERCIACKLCEAVCPAQAITIEAEEREDGSRRTTRYD 180

Query: 248 IDMTKCIYCGFCQEACPVDAIVEGPNFEFATETHEELLYDKEKLLENGDRWETEIAENLR 307
           IDMTKCIYCGFCQEACPVDAIVEGPNFEFATETHEELLYDKEKLLENGDRWETEIAENLR
Sbjct: 181 IDMTKCIYCGFCQEACPVDAIVEGPNFEFATETHEELLYDKEKLLENGDRWETEIAENLR 222

Query: 308 SESLYR 314
           SESLYR
Sbjct: 241 SESLYR 222

[Close]

BLAST of Lsi11G011030 vs. TAIR 10
Match: ATCG01090.1 (NADPH dehydrogenases )

HSP 1 Score: 80.5 bits (197), Expect = 2.7e-15
Identity = 43/121 (35.54%), Postives = 63/121 (52.07%), Query Frame = 0


Query: 169 RGLMLTLKYFFEKKVTINYPFEKGPLSPRFRGEHALRRYPTGEERCIACKLCEAICPAQA 228
           +G M+TL +     VTI YP+EK   S RFRG     R     ++CIAC++C  +CP   
Sbjct: 24  QGFMITLSHTNRLPVTIQYPYEKLITSERFRG-----RIHFEFDKCIACEVCVRVCPIDL 83

Query: 229 ITIEAE-EREDGSRRTTRYDIDMTKCIYCGFCQEACPVDAIVEGPNFEFATETHEELLYD 288
             ++ + E     +R   Y ID   CI+CG C E CP + +     +EF+T    EL Y+
Sbjct: 84  PVVDWKLETNIRKKRLLNYSIDFGICIFCGNCVEYCPTNCLSMTEEYEFSTYDRHELNYN 139

The following BLAST results are available for this feature:

BLAST of Lsi11G011030 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FX83	1.1e-106	79.67	NADH dehydrogenase [ubiquinone] iron-sulfur protein 8-B, mitochondrial OS=Arabid...	[more]
Q42599	1.6e-105	78.86	NADH dehydrogenase [ubiquinone] iron-sulfur protein 8-A, mitochondrial OS=Arabid...	[more]
P80269	4.6e-105	78.54	NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial OS=Solanum ...	[more]
O24143	5.6e-103	77.42	NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial OS=Nicotian...	[more]
O21233	7.4e-79	85.71	NADH-ubiquinone oxidoreductase subunit 8 OS=Reclinomonas americana OX=48483 GN=N...	[more]

BLAST of Lsi11G011030 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C2E8	9.3e-117	89.02	NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial OS=Momordic...	[more]
A0A6J1I8H7	5.1e-115	87.40	NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial OS=Cucurbit...	[more]
A0A6J1F0I7	5.1e-115	87.40	NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial OS=Cucurbit...	[more]
A0A6J1GJX8	5.6e-114	87.40	NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial-like OS=Cuc...	[more]
A0A0A0K5Z9	2.8e-113	87.40	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G268510 PE=3 SV=1	[more]

BLAST of Lsi11G011030 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038887775.1	3.0e-117	89.84	NADH dehydrogenase [ubiquinone] iron-sulfur protein 8-B, mitochondrial [Benincas...	[more]
XP_022136046.1	1.9e-116	89.02	NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial [Momordica ...	[more]
KAG7011706.1	5.6e-116	88.62	hypothetical protein SDJN02_26612 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022933976.1	1.1e-114	87.40	NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial [Cucurbita ...	[more]
XP_022952272.1	1.2e-113	87.40	NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial-like [Cucur...	[more]

BLAST of Lsi11G011030 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G16700.1	7.8e-108	79.67	Alpha-helical ferredoxin	[more]
AT1G79010.1	1.1e-106	78.86	Alpha-helical ferredoxin	[more]
ATCG01090.1	2.7e-15	35.54	NADPH dehydrogenases	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR017896	4Fe-4S ferredoxin-type, iron-sulphur binding domain	PFAM	PF12838	Fer4_7	coord: 213..267 e-value: 8.3E-13 score: 48.7
IPR017896	4Fe-4S ferredoxin-type, iron-sulphur binding domain	PROSITE	PS51379	4FE4S_FER_2	coord: 244..273 score: 10.742653
IPR017896	4Fe-4S ferredoxin-type, iron-sulphur binding domain	PROSITE	PS51379	4FE4S_FER_2	coord: 205..234 score: 9.994982
IPR010226	NADH-quinone oxidoreductase, chain I	TIGRFAM	TIGR01971	TIGR01971	coord: 170..291 e-value: 4.1E-52 score: 172.9
IPR010226	NADH-quinone oxidoreductase, chain I	PANTHER	PTHR10849	NADH DEHYDROGENASE UBIQUINONE IRON-SULFUR PROTEIN 8, MITOCHONDRIAL	coord: 134..313
IPR010226	NADH-quinone oxidoreductase, chain I	HAMAP	MF_01351	NDH1_NuoI	coord: 161..297 score: 29.414911
None	No IPR available	GENE3D	3.30.70.3270		coord: 187..312 e-value: 3.4E-46 score: 158.8
None	No IPR available	PANTHER	PTHR10849:SF30	SUBFAMILY NOT NAMED	coord: 134..313
None	No IPR available	SUPERFAMILY	54862	4Fe-4S ferredoxins	coord: 187..297
IPR017900	4Fe-4S ferredoxin, iron-sulphur binding, conserved site	PROSITE	PS00198	4FE4S_FER_1	coord: 253..264
IPR017900	4Fe-4S ferredoxin, iron-sulphur binding, conserved site	PROSITE	PS00198	4FE4S_FER_1	coord: 214..225

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi11G011030.1	Lsi11G011030.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006120	mitochondrial electron transport, NADH to ubiquinone
biological_process	GO:0032981	mitochondrial respiratory chain complex I assembly
cellular_component	GO:0005747	mitochondrial respiratory chain complex I
cellular_component	GO:0016020	membrane
molecular_function	GO:0051539	4 iron, 4 sulfur cluster binding
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0003954	NADH dehydrogenase activity
molecular_function	GO:0016651	oxidoreductase activity, acting on NAD(P)H