Lsi11G009060 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi11G009060
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionAmmonium transporter
Locationchr11: 12298731 .. 12301001 (-)
RNA-Seq ExpressionLsi11G009060
SyntenyLsi11G009060
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCCACTCTTTCTTGCCAACCTTCCGATTTGGTGCCACTCCTTGGCTCATCAGCCACCACCAATTCCACCGCCATAGCCGAGTTCTTATGCTCAAGATTCACCACCATAGCCACCACTTTCAGCTCCACCACCTATGCCGTCGACAACACCTACCTCCTCTTTTCTGCCTACCTCGTCTTCGCCATGCAGCTCGGCTTTGCCATGCTTTGCGCTGGCTCGGTCCGTGCCAAAAACACGATGAATATCATGCTCACCAACGTCCTCGACGCCGCTGCCGGTGGCCTCTCTTACTACCTCTTTGGCTTTGCCTTTGCCTTTGGTGCCCCCTCCAACTCCTTCATCGGCCGCCATTTTTTTGACCTCTCCAGTGTCCCTAACCCCTCCACTGACTATAGCTTCTTTCTCTATCAATGGGCCTTTGCTATTGCTGCTGCAGGTATTTATCTAAATTTTTTTATTTAAATTAAAAATTTAGTCCTATGATTTGATAATATCTCATAAACATACTCTTTATAGTTTAAAAAAATTCTCGTAAAATCATCCTAATTATAAATCATGACTACTAAAATCTAACTTTCTTTAAAATATAGGGACCAAATTTTTTCTATTCTTTCTACTTACTTATACACTTCGCCTTATCAATATTAATATTCACTCTATTTTCTTAAATTGTTTCAACTGATTTGTTTTGTCTAATTCTTCTTGAATTTTCCTTGAGTGTTCTCATTAAGAGTAATACTTTTCATGCATCTCCTATCATCATCACATGAAAAAGAATTAAAATATTTAAAAATAAAAAATAAAAAAAATTTATCACATGTCAAATTATGATTAGACAATTTGGTGGGATGCACAAAAATAGGCAATACCAACTAAGTATTTTTTGTTCGTTAATCCTAATTTTGTGTGGCTATCAATAAAGTAAATAAAGAATCTCTTCTTAGTTGAACAATTCAACAATTATAAGATGAGAAAAGTAACCTTCAACTTTAAGGGAAGGATTACATGATTACATGAGAATTATTTGCAAAAACTCAAAAAGCTTAAATTAACACACTACAATAAATTTGATGTAACAAATAGTAATTGTTTCATTCATATTCTTATCAGGCATCACCAGTGGCTCCATAGCTGAGCGAACCCAATTTGTTGCCTATCTAATCTACTCCACTATTCTAACTGGCTTCATCTACCCTATAGTCTCCCACTGGTTCTGGTCTGCTGACGGGTGGGCCAGCCCAACCCGACCCGATAACCTCATATTCAACTCAGGTGCGATCGACTTCGCCGGGTCCGGTGTGGTCCACATGGTTGGTGGAATAGCTGGTCTCTGGGGAGCTTTAATCGAAGGCCCTCGGATTGGTCGGTTCGATCGATCAGGTCGATCTGTCGCCCTTCGCGGCCACAGCGCATCACTTGTAGTTCTTGGCTCTTTCCTCCTTTGGTTTGGTTGGTACGGATTTAATCCCGGCTCATTCTTAACAATCTCCAAATCCTACAATGACGGTCGTCCTTACTACGGCCAATGGAGCGCCATAGGTCGAACTGGAGTCACCACAACACTAGCCGGATGCACAGCTGCCTTAACAACCCTCTTCGCAAAACGCCTATTAGTCGGGCATTGGAACGTAATCGACGTGTGTAATGGTCTGTTAGGCGGGTTTGCCGCCATCACATCGGGTTGCGCCGTGGTCGAGCCATGGGCGGCAGTGATATGTGGTTTCGTAGCATCATGGGTGTTAATTGGATTAAACAAGCTAGCCGAGAAGGTAAGCTACGACGACCCATTAGAGGCGGCGCAATTGCACGGCGGGTGTGGGGCATGGGGTTTAATATTCACAGGATTATTCGCAACAAAGAAATACGTCGGTGAGGTATATGAAGTCGGGCGGCCGTTCGGATTGTTGATGGGCGGCGGAGGGAAGCTGCTGGCAGCGCAGGTGGTGGAGATAGTAGCGATAATAGGGTGGGTGTCAGTAACAATGGGGGGTTTGTTTTATGGGCTGAAGAAAGTGAAACTATTAAGAATATCAAGAGAGGATGAAATGGCAGGGATGGATTTGACAAGGCATGGGGGATTTGCTTATGTATATAATGATGAGGAAGATCTATCAGAGAAGCCTTCTGGGTTTATGATGAGGAGGATTGAGCCCACTGATGCTAGTTCTCCTGAGGAGAATACTTCTTCTGGGATGGGGGCTGTTTGATTCAGATTTCGAATCCAAACGATGTCGTTTTGTACGAGTCAATGCGTTTGTTGTAGTTTAG

mRNA sequence

ATGTCCACTCTTTCTTGCCAACCTTCCGATTTGGTGCCACTCCTTGGCTCATCAGCCACCACCAATTCCACCGCCATAGCCGAGTTCTTATGCTCAAGATTCACCACCATAGCCACCACTTTCAGCTCCACCACCTATGCCGTCGACAACACCTACCTCCTCTTTTCTGCCTACCTCGTCTTCGCCATGCAGCTCGGCTTTGCCATGCTTTGCGCTGGCTCGGTCCGTGCCAAAAACACGATGAATATCATGCTCACCAACGTCCTCGACGCCGCTGCCGGTGGCCTCTCTTACTACCTCTTTGGCTTTGCCTTTGCCTTTGGCATCACCAGTGGCTCCATAGCTGAGCGAACCCAATTTGTTGCCTATCTAATCTACTCCACTATTCTAACTGGCTTCATCTACCCTATAGTCTCCCACTGGTTCTGGTCTGCTGACGGGTGGGCCAGCCCAACCCGACCCGATAACCTCATATTCAACTCAGGTGCGATCGACTTCGCCGGGTCCGGTGTGGTCCACATGGTTGGTGGAATAGCTGGTCTCTGGGGAGCTTTAATCGAAGGCCCTCGGATTGGTCGGTTCGATCGATCAGGTCGATCTGTCGCCCTTCGCGGCCACAGCGCATCACTTGTAGTTCTTGGCTCTTTCCTCCTTTGGTTTGGTTGGTACGGATTTAATCCCGGCTCATTCTTAACAATCTCCAAATCCTACAATGACGGTCGTCCTTACTACGGCCAATGGAGCGCCATAGGTCGAACTGGAGTCACCACAACACTAGCCGGATGCACAGCTGCCTTAACAACCCTCTTCGCAAAACGCCTATTAGTCGGGCATTGGAACGTAATCGACGTGTGTAATGGTCTGTTAGGCGGGTTTGCCGCCATCACATCGGGTTGCGCCGTGGTCGAGCCATGGGCGGCAGTGATATGTGGTTTCGTAGCATCATGGGTGTTAATTGGATTAAACAAGCTAGCCGAGAAGGTAAGCTACGACGACCCATTAGAGGCGGCGCAATTGCACGGCGGGTGTGGGGCATGGGGTTTAATATTCACAGGATTATTCGCAACAAAGAAATACGTCGGTGAGGTATATGAAGTCGGGCGGCCGTTCGGATTGTTGATGGGCGGCGGAGGGAAGCTGCTGGCAGCGCAGGTGGTGGAGATAGTAGCGATAATAGGGTGGGTGTCAGTAACAATGGGGGGTTTGTTTTATGGGCTGAAGAAAGTGAAACTATTAAGAATATCAAGAGAGGATGAAATGGCAGGGATGGATTTGACAAGGCATGGGGGATTTGCTTATGTATATAATGATGAGGAAGATCTATCAGAGAAGCCTTCTGGGTTTATGATGAGGAGGATTGAGCCCACTGATGCTAGTTCTCCTGAGGAGAATACTTCTTCTGGGATGGGGGCTGTTTGATTCAGATTTCGAATCCAAACGATGTCGTTTTGTACGAGTCAATGCGTTTGTTGTAGTTTAG

Coding sequence (CDS)

ATGTCCACTCTTTCTTGCCAACCTTCCGATTTGGTGCCACTCCTTGGCTCATCAGCCACCACCAATTCCACCGCCATAGCCGAGTTCTTATGCTCAAGATTCACCACCATAGCCACCACTTTCAGCTCCACCACCTATGCCGTCGACAACACCTACCTCCTCTTTTCTGCCTACCTCGTCTTCGCCATGCAGCTCGGCTTTGCCATGCTTTGCGCTGGCTCGGTCCGTGCCAAAAACACGATGAATATCATGCTCACCAACGTCCTCGACGCCGCTGCCGGTGGCCTCTCTTACTACCTCTTTGGCTTTGCCTTTGCCTTTGGCATCACCAGTGGCTCCATAGCTGAGCGAACCCAATTTGTTGCCTATCTAATCTACTCCACTATTCTAACTGGCTTCATCTACCCTATAGTCTCCCACTGGTTCTGGTCTGCTGACGGGTGGGCCAGCCCAACCCGACCCGATAACCTCATATTCAACTCAGGTGCGATCGACTTCGCCGGGTCCGGTGTGGTCCACATGGTTGGTGGAATAGCTGGTCTCTGGGGAGCTTTAATCGAAGGCCCTCGGATTGGTCGGTTCGATCGATCAGGTCGATCTGTCGCCCTTCGCGGCCACAGCGCATCACTTGTAGTTCTTGGCTCTTTCCTCCTTTGGTTTGGTTGGTACGGATTTAATCCCGGCTCATTCTTAACAATCTCCAAATCCTACAATGACGGTCGTCCTTACTACGGCCAATGGAGCGCCATAGGTCGAACTGGAGTCACCACAACACTAGCCGGATGCACAGCTGCCTTAACAACCCTCTTCGCAAAACGCCTATTAGTCGGGCATTGGAACGTAATCGACGTGTGTAATGGTCTGTTAGGCGGGTTTGCCGCCATCACATCGGGTTGCGCCGTGGTCGAGCCATGGGCGGCAGTGATATGTGGTTTCGTAGCATCATGGGTGTTAATTGGATTAAACAAGCTAGCCGAGAAGGTAAGCTACGACGACCCATTAGAGGCGGCGCAATTGCACGGCGGGTGTGGGGCATGGGGTTTAATATTCACAGGATTATTCGCAACAAAGAAATACGTCGGTGAGGTATATGAAGTCGGGCGGCCGTTCGGATTGTTGATGGGCGGCGGAGGGAAGCTGCTGGCAGCGCAGGTGGTGGAGATAGTAGCGATAATAGGGTGGGTGTCAGTAACAATGGGGGGTTTGTTTTATGGGCTGAAGAAAGTGAAACTATTAAGAATATCAAGAGAGGATGAAATGGCAGGGATGGATTTGACAAGGCATGGGGGATTTGCTTATGTATATAATGATGAGGAAGATCTATCAGAGAAGCCTTCTGGGTTTATGATGAGGAGGATTGAGCCCACTGATGCTAGTTCTCCTGAGGAGAATACTTCTTCTGGGATGGGGGCTGTTTGA

Protein sequence

MSTLSCQPSDLVPLLGSSATTNSTAIAEFLCSRFTTIATTFSSTTYAVDNTYLLFSAYLVFAMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLSYYLFGFAFAFGITSGSIAERTQFVAYLIYSTILTGFIYPIVSHWFWSADGWASPTRPDNLIFNSGAIDFAGSGVVHMVGGIAGLWGALIEGPRIGRFDRSGRSVALRGHSASLVVLGSFLLWFGWYGFNPGSFLTISKSYNDGRPYYGQWSAIGRTGVTTTLAGCTAALTTLFAKRLLVGHWNVIDVCNGLLGGFAAITSGCAVVEPWAAVICGFVASWVLIGLNKLAEKVSYDDPLEAAQLHGGCGAWGLIFTGLFATKKYVGEVYEVGRPFGLLMGGGGKLLAAQVVEIVAIIGWVSVTMGGLFYGLKKVKLLRISREDEMAGMDLTRHGGFAYVYNDEEDLSEKPSGFMMRRIEPTDASSPEENTSSGMGAV
Homology
BLAST of Lsi11G009060 vs. ExPASy Swiss-Prot
Match: Q9ZPJ8 (Ammonium transporter 1 member 2 OS=Arabidopsis thaliana OX=3702 GN=AMT1-2 PE=1 SV=1)

HSP 1 Score: 728.4 bits (1879), Expect = 5.2e-209
Identity = 371/503 (73.76%), Postives = 407/503 (80.91%), Query Frame = 0

Query: 2   STLSCQPSDLVPLLGSSA-TTNSTAIAEFLCSRFTTIATTFSSTTYAVDNTYLLFSAYLV 61
           +T +C   DL  LL SS+ +T+S A A FLCS+ + I+   S TTYAVDNTYLLFSAYLV
Sbjct: 4   ATTTCSAVDLSALLSSSSNSTSSLAAATFLCSQISNISNKLSDTTYAVDNTYLLFSAYLV 63

Query: 62  FAMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLSYYLFGFAFAF------------- 121
           FAMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAG +SYYLFGFAFAF             
Sbjct: 64  FAMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGAISYYLFGFAFAFGTPSNGFIGRHHS 123

Query: 122 -----------------------------GITSGSIAERTQFVAYLIYSTILTGFIYPIV 181
                                        GITSGSIAERTQFVAYLIYST LTGF+YP V
Sbjct: 124 FFALSSYPERPGSDFSFFLYQWAFAIAAAGITSGSIAERTQFVAYLIYSTFLTGFVYPTV 183

Query: 182 SHWFWSADGWASPTRPD-NLIFNSGAIDFAGSGVVHMVGGIAGLWGALIEGPRIGRFDRS 241
           SHWFWS+DGWAS +R D NL+F SGAIDFAGSGVVHMVGGIAGL GAL+EGPRIGRFDRS
Sbjct: 184 SHWFWSSDGWASASRSDNNLLFGSGAIDFAGSGVVHMVGGIAGLCGALVEGPRIGRFDRS 243

Query: 242 GRSVALRGHSASLVVLGSFLLWFGWYGFNPGSFLTISKSYNDGRPYYGQWSAIGRTGVTT 301
           GRSVALRGHSASLVVLG+FLLWFGWYGFNPGSFLTI K Y+  RPYYGQWSA+GRT VTT
Sbjct: 244 GRSVALRGHSASLVVLGTFLLWFGWYGFNPGSFLTILKGYDKSRPYYGQWSAVGRTAVTT 303

Query: 302 TLAGCTAALTTLFAKRLLVGHWNVIDVCNGLLGGFAAITSGCAVVEPWAAVICGFVASWV 361
           TL+GCTAALTTLF+KRLL GHWNVIDVCNGLLGGFAAITSGCAVVEPWAA++CGFVASWV
Sbjct: 304 TLSGCTAALTTLFSKRLLAGHWNVIDVCNGLLGGFAAITSGCAVVEPWAAIVCGFVASWV 363

Query: 362 LIGLNKLAEKVSYDDPLEAAQLHGGCGAWGLIFTGLFATKKYVGEVYEVGRPFGLLMGGG 421
           LIG N LA+K+ YDDPLEAAQLHGGCGAWGLIFTGLFA K+YV E+Y   RP+GL MGGG
Sbjct: 364 LIGFNLLAKKLKYDDPLEAAQLHGGCGAWGLIFTGLFARKEYVNEIYSGDRPYGLFMGGG 423

Query: 422 GKLLAAQVVEIVAIIGWVSVTMGGLFYGLKKVKLLRISREDEMAGMDLTRHGGFAYVYND 461
           GKLLAAQ+V+I+ I+GWV+VTMG LFYGL K+ LLRIS EDEMAGMD+TRHGGFAY YND
Sbjct: 424 GKLLAAQIVQIIVIVGWVTVTMGPLFYGLHKMNLLRISAEDEMAGMDMTRHGGFAYAYND 483

BLAST of Lsi11G009060 vs. ExPASy Swiss-Prot
Match: O04161 (Ammonium transporter 1 member 2 OS=Solanum lycopersicum OX=4081 GN=AMT1-2 PE=2 SV=1)

HSP 1 Score: 711.8 bits (1836), Expect = 5.0e-204
Identity = 367/510 (71.96%), Postives = 407/510 (79.80%), Query Frame = 0

Query: 2   STLSCQPSDLVPLLGSSATTNSTAIAEFLCSRFTTIATTFSSTTYAVDNTYLLFSAYLVF 61
           S ++C  ++L P LGSSA  N+TA AEF+CSRF+ ++   ++TTYAVD TYLLFSAYLVF
Sbjct: 3   SAMTCSAAELFPHLGSSA--NATAAAEFICSRFSAVSEYLTNTTYAVDTTYLLFSAYLVF 62

Query: 62  AMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLSYYLFGFAFAF-------------- 121
           AMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGG SYYLFGFAFAF              
Sbjct: 63  AMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGGFSYYLFGFAFAFGAPSNGFIGKHFFG 122

Query: 122 -------------------------GITSGSIAERTQFVAYLIYSTILTGFIYPIVSHWF 181
                                    GITSGSIAERTQFVAYLIYS+ LTGF+YPIVSHWF
Sbjct: 123 LKEFPSQAFDYSYFLYQWAFAIAAAGITSGSIAERTQFVAYLIYSSFLTGFVYPIVSHWF 182

Query: 182 WSADGWASPTRPD-NLIFNSGAIDFAGSGVVHMVGGIAGLWGALIEGPRIGRFDRSGRSV 241
           WS DGWAS ++ D NL+   G IDFAGSGVVHMVGGIAGLWGA IEGPRIGRFDRSGRSV
Sbjct: 183 WSGDGWASASKTDGNLLLRFGVIDFAGSGVVHMVGGIAGLWGAFIEGPRIGRFDRSGRSV 242

Query: 242 ALRGHSASLVVLGSFLLWFGWYGFNPGSFLTISKSYND--GRPYYGQWSAIGRTGVTTTL 301
           ALRGHSASLVVLG+FLLWFGWYGFNPGSFLTI KSY+      YYGQWSAIGRT VTTTL
Sbjct: 243 ALRGHSASLVVLGTFLLWFGWYGFNPGSFLTILKSYDHTIRGTYYGQWSAIGRTAVTTTL 302

Query: 302 AGCTAALTTLFAKRLLVGHWNVIDVCNGLLGGFAAITSGCAVVEPWAAVICGFVASWVLI 361
           AGCTAALTTLF KRLLV HWNV+DVCNGLLGGFAAITSGCAVVEPWAA++CGF+A+WVLI
Sbjct: 303 AGCTAALTTLFCKRLLVAHWNVVDVCNGLLGGFAAITSGCAVVEPWAAIVCGFIAAWVLI 362

Query: 362 GLNKLAEKVSYDDPLEAAQLHGGCGAWGLIFTGLFATKKYVGEVYE--VGRPFGLLMGGG 421
           G N LA K+ YDDPLEAAQLHGGCG+WG+IFTGLFA K+YV EVY     RP+GL MGGG
Sbjct: 363 GFNALAAKLKYDDPLEAAQLHGGCGSWGIIFTGLFAKKEYVNEVYPGFPNRPYGLFMGGG 422

Query: 422 GKLLAAQVVEIVAIIGWVSVTMGGLFYGLKKVKLLRISREDEMAGMDLTRHGGFAYVYND 468
           GKLL AQV+++V IIGWVSVTMG LFY L K KLLRISR+DE AGMDLTRHGGFAY+Y+D
Sbjct: 423 GKLLGAQVIQVVVIIGWVSVTMGPLFYLLHKFKLLRISRDDETAGMDLTRHGGFAYIYHD 482

BLAST of Lsi11G009060 vs. ExPASy Swiss-Prot
Match: Q9SVT8 (Ammonium transporter 1 member 4 OS=Arabidopsis thaliana OX=3702 GN=AMT1-4 PE=1 SV=1)

HSP 1 Score: 669.5 bits (1726), Expect = 2.9e-191
Identity = 340/496 (68.55%), Postives = 390/496 (78.63%), Query Frame = 0

Query: 2   STLSCQPSDLVPLL-GSSATTNSTAIAEFLCSRFTTIATTFSSTTYAVDNTYLLFSAYLV 61
           S LSC  SDL+PLL G +  T + A AE++C RF T+A  F+   YA+DNTYLLFSAYLV
Sbjct: 3   SALSCSASDLIPLLSGGANATAAAAAAEYICGRFDTVAGKFTDAAYAIDNTYLLFSAYLV 62

Query: 62  FAMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLSYYLFGFAFAF------------- 121
           FAMQLGFAMLCAGSVRAKNTMNIMLTNV+DAAAGGL YYLFGFAFAF             
Sbjct: 63  FAMQLGFAMLCAGSVRAKNTMNIMLTNVIDAAAGGLFYYLFGFAFAFGSPSNGFIGKHFF 122

Query: 122 --------------------------GITSGSIAERTQFVAYLIYSTILTGFIYPIVSHW 181
                                     GITSGSIAERTQFVAYLIYS+ LTG +YPIVSHW
Sbjct: 123 GMYDFPQPTFDYPYFLYQWTFAIAAAGITSGSIAERTQFVAYLIYSSFLTGLVYPIVSHW 182

Query: 182 FWSADGWASPTRPDNLIFNSGAIDFAGSGVVHMVGGIAGLWGALIEGPRIGRFDRSGRSV 241
           FWS+DGWASP R +NL+F SG IDFAGSGVVHMVGGIAGLWGALIEGPRIGRF   G+ V
Sbjct: 183 FWSSDGWASPARSENLLFQSGVIDFAGSGVVHMVGGIAGLWGALIEGPRIGRFGVGGKPV 242

Query: 242 ALRGHSASLVVLGSFLLWFGWYGFNPGSFLTISKSYND--GRPYYGQWSAIGRTGVTTTL 301
            LRGHSA+LVVLG+FLLWFGWYGFNPGSF TI K+Y +  G  +YGQWSA+GRT VTTTL
Sbjct: 243 TLRGHSATLVVLGTFLLWFGWYGFNPGSFATIFKAYGETPGSSFYGQWSAVGRTAVTTTL 302

Query: 302 AGCTAALTTLFAKRLLVGHWNVIDVCNGLLGGFAAITSGCAVVEPWAAVICGFVASWVLI 361
           AGCTAALTTLF KRL+ G+WNV DVCNGLLGGFAAITSGC+VVEPWAA++CGFVA+WVL+
Sbjct: 303 AGCTAALTTLFGKRLIDGYWNVTDVCNGLLGGFAAITSGCSVVEPWAALVCGFVAAWVLM 362

Query: 362 GLNKLAEKVSYDDPLEAAQLHGGCGAWGLIFTGLFATKKYVGEVY--EVGRPFGLLMGGG 421
           G N+LAEK+ +DDPLEAAQLHGGCGAWG+IFTGLFA K+Y+ E++  +  RPFGLLMGGG
Sbjct: 363 GCNRLAEKLQFDDPLEAAQLHGGCGAWGIIFTGLFAEKRYIAEIFGGDPNRPFGLLMGGG 422

Query: 422 GKLLAAQVVEIVAIIGWVSVTMGGLFYGLKKVKLLRISREDEMAGMDLTRHGGFAYVYND 454
           G+LLAA VV+I+ I GWVSVTMG LF+ L K+KLLRI  EDE+AG+D T HGG AY+Y +
Sbjct: 423 GRLLAAHVVQILVITGWVSVTMGTLFFILHKLKLLRIPAEDEIAGVDPTSHGGLAYMYTE 482

BLAST of Lsi11G009060 vs. ExPASy Swiss-Prot
Match: P54144 (Ammonium transporter 1 member 1 OS=Arabidopsis thaliana OX=3702 GN=AMT1-1 PE=1 SV=1)

HSP 1 Score: 662.1 bits (1707), Expect = 4.6e-189
Identity = 341/505 (67.52%), Postives = 388/505 (76.83%), Query Frame = 0

Query: 4   LSCQPSDLVPLLGSSATTNSTAIAEFLCSRFTTIATTFSSTTYAVDNTYLLFSAYLVFAM 63
           +SC  +DL  LLG     N+TA A ++C +   +   F  T +A+DNTYLLFSAYLVF+M
Sbjct: 1   MSCSATDLAVLLG----PNATAAANYICGQLGDVNNKFIDTAFAIDNTYLLFSAYLVFSM 60

Query: 64  QLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLSYYLFGFAFAF---------------- 123
           QLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGGL YYLFG+AFAF                
Sbjct: 61  QLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLFYYLFGYAFAFGSPSNGFIGKHYFGLK 120

Query: 124 -----------------------GITSGSIAERTQFVAYLIYSTILTGFIYPIVSHWFWS 183
                                  GITSGSIAERTQFVAYLIYS+ LTGF+YP+VSHWFWS
Sbjct: 121 DIPTASADYSNFLYQWAFAIAAAGITSGSIAERTQFVAYLIYSSFLTGFVYPVVSHWFWS 180

Query: 184 ADGWASPTRPD-NLIFNSGAIDFAGSGVVHMVGGIAGLWGALIEGPRIGRFDRSGRSVAL 243
            DGWASP R D +L+F++GAIDFAGSGVVHMVGGIAGLWGALIEGPR+GRFD  GR++AL
Sbjct: 181 VDGWASPFRTDGDLLFSTGAIDFAGSGVVHMVGGIAGLWGALIEGPRLGRFDNGGRAIAL 240

Query: 244 RGHSASLVVLGSFLLWFGWYGFNPGSFLTISKSYNDGRPYYGQWSAIGRTGVTTTLAGCT 303
           RGHSASLVVLG+FLLWFGWYGFNPGSF  I  +Y  G  Y GQWSA+GRT VTTTLAGCT
Sbjct: 241 RGHSASLVVLGTFLLWFGWYGFNPGSFNKILVTYETG-TYNGQWSAVGRTAVTTTLAGCT 300

Query: 304 AALTTLFAKRLLVGHWNVIDVCNGLLGGFAAITSGCAVVEPWAAVICGFVASWVLIGLNK 363
           AALTTLF KRLL GHWNV DVCNGLLGGFAAIT GC+VVEPWAA+ICGFVA+ VL+G NK
Sbjct: 301 AALTTLFGKRLLSGHWNVTDVCNGLLGGFAAITGGCSVVEPWAAIICGFVAALVLLGCNK 360

Query: 364 LAEKVSYDDPLEAAQLHGGCGAWGLIFTGLFATKKYVGEVY--EVGRPFGLLMGGGGKLL 423
           LAEK+ YDDPLEAAQLHGGCGAWGLIFT LFA +KY+ ++Y  + GRP GL MGGGGKLL
Sbjct: 361 LAEKLKYDDPLEAAQLHGGCGAWGLIFTALFAQEKYLNQIYGNKPGRPHGLFMGGGGKLL 420

Query: 424 AAQVVEIVAIIGWVSVTMGGLFYGLKKVKLLRISREDEMAGMDLTRHGGFAYVYNDEEDL 467
            AQ+++I+ I GWVS TMG LF+ LKK+KLLRIS EDEMAGMD+TRHGGFAY+Y D++  
Sbjct: 421 GAQLIQIIVITGWVSATMGTLFFILKKMKLLRISSEDEMAGMDMTRHGGFAYMYFDDD-- 480

BLAST of Lsi11G009060 vs. ExPASy Swiss-Prot
Match: Q7XQ12 (Ammonium transporter 1 member 1 OS=Oryza sativa subsp. japonica OX=39947 GN=AMT1-1 PE=2 SV=1)

HSP 1 Score: 652.5 bits (1682), Expect = 3.6e-186
Identity = 339/501 (67.66%), Postives = 383/501 (76.45%), Query Frame = 0

Query: 9   SDLVPLLGSSATTNSTAIAEFLCSRFTTIATTFSSTTYAVDNTYLLFSAYLVFAMQLGFA 68
           +DL PLLG  A  N+T   ++LC+R       F+ TT AVD TYLLFSAYLVFAMQLGFA
Sbjct: 6   ADLAPLLGPVA-ANAT---DYLCNR-------FADTTSAVDATYLLFSAYLVFAMQLGFA 65

Query: 69  MLCAGSVRAKNTMNIMLTNVLDAAAGGLSYYLFGFAFAF--------------------- 128
           MLCAGSVRAKNTMNIMLTNVLDAAAG L YYLFGFAFAF                     
Sbjct: 66  MLCAGSVRAKNTMNIMLTNVLDAAAGALFYYLFGFAFAFGTPSNGFIGKQFFGLKHMPQT 125

Query: 129 ------------------GITSGSIAERTQFVAYLIYSTILTGFIYPIVSHWFWSADGWA 188
                             GITSGSIAERTQFVAYLIYS  LTGF+YP+VSHW WSADGWA
Sbjct: 126 GFDYDFFLFQWAFAIAAAGITSGSIAERTQFVAYLIYSAFLTGFVYPVVSHWIWSADGWA 185

Query: 189 SPTRPDN-LIFNSGAIDFAGSGVVHMVGGIAGLWGALIEGPRIGRFDRSGRSVALRGHSA 248
           S +R    L+F SG IDFAGSGVVHMVGG+AGLWGALIEGPRIGRFD +GRSVAL+GHSA
Sbjct: 186 SASRTSGPLLFGSGVIDFAGSGVVHMVGGVAGLWGALIEGPRIGRFDHAGRSVALKGHSA 245

Query: 249 SLVVLGSFLLWFGWYGFNPGSFLTISKSYNDGRPYYGQWSAIGRTGVTTTLAGCTAALTT 308
           SLVVLG+FLLWFGWYGFNPGSF TI K+Y       GQWS +GRT VTTTLAG  AALTT
Sbjct: 246 SLVVLGTFLLWFGWYGFNPGSFTTILKTYGPAGGINGQWSGVGRTAVTTTLAGSVAALTT 305

Query: 309 LFAKRLLVGHWNVIDVCNGLLGGFAAITSGCAVVEPWAAVICGFVASWVLIGLNKLAEKV 368
           LF KRL  GHWNV+DVCNGLLGGFAAIT+GC+VV+PWAA+ICGFV++WVLIGLN LA ++
Sbjct: 306 LFGKRLQTGHWNVVDVCNGLLGGFAAITAGCSVVDPWAAIICGFVSAWVLIGLNALAARL 365

Query: 369 SYDDPLEAAQLHGGCGAWGLIFTGLFATKKYVGEVYEVGRPFGLLMGGGGKLLAAQVVEI 428
            +DDPLEAAQLHGGCGAWG++FT LFA +KYV E+Y  GRP+GL MGGGGKLLAA V++I
Sbjct: 366 KFDDPLEAAQLHGGCGAWGILFTALFARQKYVEEIYGAGRPYGLFMGGGGKLLAAHVIQI 425

Query: 429 VAIIGWVSVTMGGLFYGLKKVKLLRISREDEMAGMDLTRHGGFAYVYNDEEDLSEK-PSG 465
           + I GWVS TMG LFYGLKK+ LLRIS EDE +GMDLTRHGGFAYVY+DE++  +    G
Sbjct: 426 LVIFGWVSCTMGPLFYGLKKLGLLRISAEDETSGMDLTRHGGFAYVYHDEDEHDKSGVGG 485

BLAST of Lsi11G009060 vs. ExPASy TrEMBL
Match: A0A5D3DXN7 (Ammonium transporter OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold165G00030 PE=3 SV=1)

HSP 1 Score: 874.8 bits (2259), Expect = 1.6e-250
Identity = 443/511 (86.69%), Postives = 463/511 (90.61%), Query Frame = 0

Query: 1   MSTLSCQPSDLVPLLGSSATTNSTAIAEFLCSRFTTIATTFSSTTYAVDNTYLLFSAYLV 60
           M+TLSCQPSDL+PLLGSSATTNSTA+AEFLCSRFTTIATTFSSTTYA+DNTYLLFSAYLV
Sbjct: 1   MATLSCQPSDLIPLLGSSATTNSTAVAEFLCSRFTTIATTFSSTTYAIDNTYLLFSAYLV 60

Query: 61  FAMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLSYYLFGFAFAF------------- 120
           FAMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLSYYLFGFAFAF             
Sbjct: 61  FAMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLSYYLFGFAFAFGAPSNSFIGRHFF 120

Query: 121 --------------------------GITSGSIAERTQFVAYLIYSTILTGFIYPIVSHW 180
                                     GITSGSIAERT+FVAYLIYSTILTGFIYPIVSHW
Sbjct: 121 DLSSVPNPSTDYSFFLYQWAFAIAAAGITSGSIAERTRFVAYLIYSTILTGFIYPIVSHW 180

Query: 181 FWSADGWASPTRPDNLIFNSGAIDFAGSGVVHMVGGIAGLWGALIEGPRIGRFDRSGRSV 240
           FWSADGWASPTR +NL+FNSGAIDFAGSGVVHMVGG+AGLWGALIEGPRIGRFDRSGRSV
Sbjct: 181 FWSADGWASPTRSNNLLFNSGAIDFAGSGVVHMVGGVAGLWGALIEGPRIGRFDRSGRSV 240

Query: 241 ALRGHSASLVVLGSFLLWFGWYGFNPGSFLTISKSYNDGRPYYGQWSAIGRTGVTTTLAG 300
           ALRGHSASLVVLGSFLLWFGWYGFNPGSFLTISKSYNDGRPYYGQWSAIGRTGVTTTLAG
Sbjct: 241 ALRGHSASLVVLGSFLLWFGWYGFNPGSFLTISKSYNDGRPYYGQWSAIGRTGVTTTLAG 300

Query: 301 CTAALTTLFAKRLLVGHWNVIDVCNGLLGGFAAITSGCAVVEPWAAVICGFVASWVLIGL 360
           CTAALTTLFAKRLLVGHWNVIDVCNGLLGGFAAITSGCAVVEPWAAVICGFVASWVLIG 
Sbjct: 301 CTAALTTLFAKRLLVGHWNVIDVCNGLLGGFAAITSGCAVVEPWAAVICGFVASWVLIGF 360

Query: 361 NKLAEKVSYDDPLEAAQLHGGCGAWGLIFTGLFATKKYVGEVYEVGRPFGLLMGGGGKLL 420
           NKLAE++S+DDPLEAAQLHGGCGAWGLIFTGLFATKKYVGEVY+VGRP+GLLMGGG +LL
Sbjct: 361 NKLAERLSFDDPLEAAQLHGGCGAWGLIFTGLFATKKYVGEVYQVGRPYGLLMGGGWRLL 420

Query: 421 AAQVVEIVAIIGWVSVTMGGLFYGLKKVKLLRISREDEMAGMDLTRHGGFAYVYNDEEDL 473
           AAQVVEIVAI GWVS+TMGGLFYGL K KLLRISREDEMAGMDLTRHGGFAYVYNDEED+
Sbjct: 421 AAQVVEIVAIFGWVSLTMGGLFYGLMKGKLLRISREDEMAGMDLTRHGGFAYVYNDEEDI 480

BLAST of Lsi11G009060 vs. ExPASy TrEMBL
Match: A0A1S3B4A3 (Ammonium transporter OS=Cucumis melo OX=3656 GN=LOC103485850 PE=3 SV=1)

HSP 1 Score: 874.8 bits (2259), Expect = 1.6e-250
Identity = 443/511 (86.69%), Postives = 463/511 (90.61%), Query Frame = 0

Query: 1   MSTLSCQPSDLVPLLGSSATTNSTAIAEFLCSRFTTIATTFSSTTYAVDNTYLLFSAYLV 60
           M+TLSCQPSDL+PLLGSSATTNSTA+AEFLCSRFTTIATTFSSTTYA+DNTYLLFSAYLV
Sbjct: 1   MATLSCQPSDLIPLLGSSATTNSTAVAEFLCSRFTTIATTFSSTTYAIDNTYLLFSAYLV 60

Query: 61  FAMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLSYYLFGFAFAF------------- 120
           FAMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLSYYLFGFAFAF             
Sbjct: 61  FAMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLSYYLFGFAFAFGAPSNSFIGRHFF 120

Query: 121 --------------------------GITSGSIAERTQFVAYLIYSTILTGFIYPIVSHW 180
                                     GITSGSIAERT+FVAYLIYSTILTGFIYPIVSHW
Sbjct: 121 DLSSVPNPSTDYSFFLYQWAFAIAAAGITSGSIAERTRFVAYLIYSTILTGFIYPIVSHW 180

Query: 181 FWSADGWASPTRPDNLIFNSGAIDFAGSGVVHMVGGIAGLWGALIEGPRIGRFDRSGRSV 240
           FWSADGWASPTR +NL+FNSGAIDFAGSGVVHMVGG+AGLWGALIEGPRIGRFDRSGRSV
Sbjct: 181 FWSADGWASPTRSNNLLFNSGAIDFAGSGVVHMVGGVAGLWGALIEGPRIGRFDRSGRSV 240

Query: 241 ALRGHSASLVVLGSFLLWFGWYGFNPGSFLTISKSYNDGRPYYGQWSAIGRTGVTTTLAG 300
           ALRGHSASLVVLGSFLLWFGWYGFNPGSFLTISKSYNDGRPYYGQWSAIGRTGVTTTLAG
Sbjct: 241 ALRGHSASLVVLGSFLLWFGWYGFNPGSFLTISKSYNDGRPYYGQWSAIGRTGVTTTLAG 300

Query: 301 CTAALTTLFAKRLLVGHWNVIDVCNGLLGGFAAITSGCAVVEPWAAVICGFVASWVLIGL 360
           CTAALTTLFAKRLLVGHWNVIDVCNGLLGGFAAITSGCAVVEPWAAVICGFVASWVLIG 
Sbjct: 301 CTAALTTLFAKRLLVGHWNVIDVCNGLLGGFAAITSGCAVVEPWAAVICGFVASWVLIGF 360

Query: 361 NKLAEKVSYDDPLEAAQLHGGCGAWGLIFTGLFATKKYVGEVYEVGRPFGLLMGGGGKLL 420
           NKLAE++S+DDPLEAAQLHGGCGAWGLIFTGLFATKKYVGEVY+VGRP+GLLMGGG +LL
Sbjct: 361 NKLAERLSFDDPLEAAQLHGGCGAWGLIFTGLFATKKYVGEVYQVGRPYGLLMGGGWRLL 420

Query: 421 AAQVVEIVAIIGWVSVTMGGLFYGLKKVKLLRISREDEMAGMDLTRHGGFAYVYNDEEDL 473
           AAQVVEIVAI GWVS+TMGGLFYGL K KLLRISREDEMAGMDLTRHGGFAYVYNDEED+
Sbjct: 421 AAQVVEIVAIFGWVSLTMGGLFYGLMKGKLLRISREDEMAGMDLTRHGGFAYVYNDEEDI 480

BLAST of Lsi11G009060 vs. ExPASy TrEMBL
Match: A0A0A0LKM7 (Ammonium transporter OS=Cucumis sativus OX=3659 GN=Csa_2G079100 PE=3 SV=1)

HSP 1 Score: 869.4 bits (2245), Expect = 6.9e-249
Identity = 439/508 (86.42%), Postives = 460/508 (90.55%), Query Frame = 0

Query: 1   MSTLSCQPSDLVPLLGSSATTNSTAIAEFLCSRFTTIATTFSSTTYAVDNTYLLFSAYLV 60
           M+TLSCQPSDL+PLLGSSATTNSTA+AEFLCSRFTTIA+TFSSTTYAVDN YLLFSAYLV
Sbjct: 1   MATLSCQPSDLIPLLGSSATTNSTAVAEFLCSRFTTIASTFSSTTYAVDNNYLLFSAYLV 60

Query: 61  FAMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLSYYLFGFAFAF------------- 120
           FAMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLSYYLFGFAFAF             
Sbjct: 61  FAMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLSYYLFGFAFAFGAPSNSFIGRHFF 120

Query: 121 --------------------------GITSGSIAERTQFVAYLIYSTILTGFIYPIVSHW 180
                                     GITSGSIAERTQFVAYLIYST+LTGFIYPIVSHW
Sbjct: 121 DLSSVPNPSTDYSFFLYQWAFAIATAGITSGSIAERTQFVAYLIYSTVLTGFIYPIVSHW 180

Query: 181 FWSADGWASPTRPDNLIFNSGAIDFAGSGVVHMVGGIAGLWGALIEGPRIGRFDRSGRSV 240
           FWSADGWASPTRP+NL+FNSGAIDFAGSGVVHMVGG+AGLWG+LIEGPRIGRFDRSGRSV
Sbjct: 181 FWSADGWASPTRPNNLLFNSGAIDFAGSGVVHMVGGVAGLWGSLIEGPRIGRFDRSGRSV 240

Query: 241 ALRGHSASLVVLGSFLLWFGWYGFNPGSFLTISKSYNDGRPYYGQWSAIGRTGVTTTLAG 300
           ALRGHSASLVVLGSFLLWFGWYGFNPGSFLTISKSYNDGRPYYGQWSAIGRTGVTTTLAG
Sbjct: 241 ALRGHSASLVVLGSFLLWFGWYGFNPGSFLTISKSYNDGRPYYGQWSAIGRTGVTTTLAG 300

Query: 301 CTAALTTLFAKRLLVGHWNVIDVCNGLLGGFAAITSGCAVVEPWAAVICGFVASWVLIGL 360
           CTAALTTLFAKRLLVGHWNVIDVCNGLLGGFAAITSGCAVVEPWAAVICGFVASWVLIG 
Sbjct: 301 CTAALTTLFAKRLLVGHWNVIDVCNGLLGGFAAITSGCAVVEPWAAVICGFVASWVLIGF 360

Query: 361 NKLAEKVSYDDPLEAAQLHGGCGAWGLIFTGLFATKKYVGEVYEVGRPFGLLMGGGGKLL 420
           NKLAE++S+DDPLEAAQLHGGCGAWGLIFTGLFATKKYVGEVY++GRP+GLLMGGG KLL
Sbjct: 361 NKLAERLSFDDPLEAAQLHGGCGAWGLIFTGLFATKKYVGEVYQLGRPYGLLMGGGWKLL 420

Query: 421 AAQVVEIVAIIGWVSVTMGGLFYGLKKVKLLRISREDEMAGMDLTRHGGFAYVYNDEEDL 470
           AAQVVEIVAI GWVS+TMGGLFYGL K KLLRISREDEMAGMDLTRHGGFAYVYNDEED 
Sbjct: 421 AAQVVEIVAIFGWVSLTMGGLFYGLMKGKLLRISREDEMAGMDLTRHGGFAYVYNDEEDF 480

BLAST of Lsi11G009060 vs. ExPASy TrEMBL
Match: A0A5A7TBL1 (Ammonium transporter OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold165G00100 PE=3 SV=1)

HSP 1 Score: 860.9 bits (2223), Expect = 2.5e-246
Identity = 436/511 (85.32%), Postives = 455/511 (89.04%), Query Frame = 0

Query: 1   MSTLSCQPSDLVPLLGSSATTNSTAIAEFLCSRFTTIATTFSSTTYAVDNTYLLFSAYLV 60
           M+TLSCQPSDLVPLLGSSAT NSTAIAEFLCSRFTTIATTFSSTTYAVDNTYLLFSAYLV
Sbjct: 1   MATLSCQPSDLVPLLGSSATANSTAIAEFLCSRFTTIATTFSSTTYAVDNTYLLFSAYLV 60

Query: 61  FAMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLSYYLFGFAFAF------------- 120
           FAMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLSYYLFGFAFAF             
Sbjct: 61  FAMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLSYYLFGFAFAFGAPSNSFIGRHFF 120

Query: 121 --------------------------GITSGSIAERTQFVAYLIYSTILTGFIYPIVSHW 180
                                     GITSGSIAERTQFVAYLIYS+ILTGF+YPIVSHW
Sbjct: 121 GLSNFPNPSTDYSFFLYQWAFAIAAAGITSGSIAERTQFVAYLIYSSILTGFVYPIVSHW 180

Query: 181 FWSADGWASPTRPDNLIFNSGAIDFAGSGVVHMVGGIAGLWGALIEGPRIGRFDRSGRSV 240
           FWS+DGWASPTRP NL+FNSGAIDFAGSGVVHMVGGIAGLWGALIEGPRIGRFDRSGRSV
Sbjct: 181 FWSSDGWASPTRPSNLLFNSGAIDFAGSGVVHMVGGIAGLWGALIEGPRIGRFDRSGRSV 240

Query: 241 ALRGHSASLVVLGSFLLWFGWYGFNPGSFLTISKSYNDGRPYYGQWSAIGRTGVTTTLAG 300
           ALRGHSASLVVLGSFLLWFGWYGFNPGSFLTISKSYNDGRPYYGQWSAIGRTGVTTTLAG
Sbjct: 241 ALRGHSASLVVLGSFLLWFGWYGFNPGSFLTISKSYNDGRPYYGQWSAIGRTGVTTTLAG 300

Query: 301 CTAALTTLFAKRLLVGHWNVIDVCNGLLGGFAAITSGCAVVEPWAAVICGFVASWVLIGL 360
           CTAALTTLF KRLLVGHWNVIDVCNGLLGGFAAITSGCAVVEPWAAVICGFVASWVLI  
Sbjct: 301 CTAALTTLFTKRLLVGHWNVIDVCNGLLGGFAAITSGCAVVEPWAAVICGFVASWVLISF 360

Query: 361 NKLAEKVSYDDPLEAAQLHGGCGAWGLIFTGLFATKKYVGEVYEVGRPFGLLMGGGGKLL 420
           NKLAEKV+YDDPLEAAQLHGGCGAWGLIFTGLFA K+YV EVY+ GRP+GL MGGGG+LL
Sbjct: 361 NKLAEKVNYDDPLEAAQLHGGCGAWGLIFTGLFARKEYVAEVYQAGRPYGLFMGGGGRLL 420

Query: 421 AAQVVEIVAIIGWVSVTMGGLFYGLKKVKLLRISREDEMAGMDLTRHGGFAYVYNDEEDL 473
           AAQ++EI+AIIGWVSVTMGGLFY LKKVKLLRISREDEMAGMDLTRHGGFAYVYNDE+D 
Sbjct: 421 AAQLIEILAIIGWVSVTMGGLFYALKKVKLLRISREDEMAGMDLTRHGGFAYVYNDEDDP 480

BLAST of Lsi11G009060 vs. ExPASy TrEMBL
Match: A0A1S3B4X2 (Ammonium transporter OS=Cucumis melo OX=3656 GN=LOC103485853 PE=3 SV=1)

HSP 1 Score: 860.9 bits (2223), Expect = 2.5e-246
Identity = 436/511 (85.32%), Postives = 455/511 (89.04%), Query Frame = 0

Query: 1   MSTLSCQPSDLVPLLGSSATTNSTAIAEFLCSRFTTIATTFSSTTYAVDNTYLLFSAYLV 60
           M+TLSCQPSDLVPLLGSSAT NSTAIAEFLCSRFTTIATTFSSTTYAVDNTYLLFSAYLV
Sbjct: 1   MATLSCQPSDLVPLLGSSATANSTAIAEFLCSRFTTIATTFSSTTYAVDNTYLLFSAYLV 60

Query: 61  FAMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLSYYLFGFAFAF------------- 120
           FAMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLSYYLFGFAFAF             
Sbjct: 61  FAMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLSYYLFGFAFAFGAPSNSFIGRHFF 120

Query: 121 --------------------------GITSGSIAERTQFVAYLIYSTILTGFIYPIVSHW 180
                                     GITSGSIAERTQFVAYLIYS+ILTGF+YPIVSHW
Sbjct: 121 GLSNFPNPSTDYSFFLYQWAFAIAAAGITSGSIAERTQFVAYLIYSSILTGFVYPIVSHW 180

Query: 181 FWSADGWASPTRPDNLIFNSGAIDFAGSGVVHMVGGIAGLWGALIEGPRIGRFDRSGRSV 240
           FWS+DGWASPTRP NL+FNSGAIDFAGSGVVHMVGGIAGLWGALIEGPRIGRFDRSGRSV
Sbjct: 181 FWSSDGWASPTRPSNLLFNSGAIDFAGSGVVHMVGGIAGLWGALIEGPRIGRFDRSGRSV 240

Query: 241 ALRGHSASLVVLGSFLLWFGWYGFNPGSFLTISKSYNDGRPYYGQWSAIGRTGVTTTLAG 300
           ALRGHSASLVVLGSFLLWFGWYGFNPGSFLTISKSYNDGRPYYGQWSAIGRTGVTTTLAG
Sbjct: 241 ALRGHSASLVVLGSFLLWFGWYGFNPGSFLTISKSYNDGRPYYGQWSAIGRTGVTTTLAG 300

Query: 301 CTAALTTLFAKRLLVGHWNVIDVCNGLLGGFAAITSGCAVVEPWAAVICGFVASWVLIGL 360
           CTAALTTLF KRLLVGHWNVIDVCNGLLGGFAAITSGCAVVEPWAAVICGFVASWVLI  
Sbjct: 301 CTAALTTLFTKRLLVGHWNVIDVCNGLLGGFAAITSGCAVVEPWAAVICGFVASWVLISF 360

Query: 361 NKLAEKVSYDDPLEAAQLHGGCGAWGLIFTGLFATKKYVGEVYEVGRPFGLLMGGGGKLL 420
           NKLAEKV+YDDPLEAAQLHGGCGAWGLIFTGLFA K+YV EVY+ GRP+GL MGGGG+LL
Sbjct: 361 NKLAEKVNYDDPLEAAQLHGGCGAWGLIFTGLFARKEYVAEVYQAGRPYGLFMGGGGRLL 420

Query: 421 AAQVVEIVAIIGWVSVTMGGLFYGLKKVKLLRISREDEMAGMDLTRHGGFAYVYNDEEDL 473
           AAQ++EI+AIIGWVSVTMGGLFY LKKVKLLRISREDEMAGMDLTRHGGFAYVYNDE+D 
Sbjct: 421 AAQLIEILAIIGWVSVTMGGLFYALKKVKLLRISREDEMAGMDLTRHGGFAYVYNDEDDP 480

BLAST of Lsi11G009060 vs. NCBI nr
Match: XP_038890689.1 (ammonium transporter 1 member 2-like [Benincasa hispida])

HSP 1 Score: 895.2 bits (2312), Expect = 2.4e-256
Identity = 456/511 (89.24%), Postives = 466/511 (91.19%), Query Frame = 0

Query: 1   MSTLSCQPSDLVPLLGSSATTNSTAIAEFLCSRFTTIATTFSSTTYAVDNTYLLFSAYLV 60
           M+TLSC+PSDL PLLGS+ATTNSTAIAEFLCSRFTTIATTF+STTYAVDNTYLLFSAYLV
Sbjct: 1   MATLSCRPSDLAPLLGSTATTNSTAIAEFLCSRFTTIATTFNSTTYAVDNTYLLFSAYLV 60

Query: 61  FAMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLSYYLFGFAFAF------------- 120
           FAMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLSYYLFGFAFAF             
Sbjct: 61  FAMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLSYYLFGFAFAFGAPSNSFIGRHFF 120

Query: 121 --------------------------GITSGSIAERTQFVAYLIYSTILTGFIYPIVSHW 180
                                     GITSGSIAERTQFVAYLIYSTILTGFIYPIVSHW
Sbjct: 121 DLSDIPNPSTDYSFFLYQWAFAIAAAGITSGSIAERTQFVAYLIYSTILTGFIYPIVSHW 180

Query: 181 FWSADGWASPTRPDNLIFNSGAIDFAGSGVVHMVGGIAGLWGALIEGPRIGRFDRSGRSV 240
           FWSADGWASPTRP NL+FNSGAIDFAGSGVVHMVGGIAGLWGALIEGPRIGRFDRSGRSV
Sbjct: 181 FWSADGWASPTRPSNLLFNSGAIDFAGSGVVHMVGGIAGLWGALIEGPRIGRFDRSGRSV 240

Query: 241 ALRGHSASLVVLGSFLLWFGWYGFNPGSFLTISKSYNDGRPYYGQWSAIGRTGVTTTLAG 300
           ALRGHSASLVVLGSFLLWFGWYGFNPGSFLTISKSYNDGRPYYGQWSAIGRTGVTTTLAG
Sbjct: 241 ALRGHSASLVVLGSFLLWFGWYGFNPGSFLTISKSYNDGRPYYGQWSAIGRTGVTTTLAG 300

Query: 301 CTAALTTLFAKRLLVGHWNVIDVCNGLLGGFAAITSGCAVVEPWAAVICGFVASWVLIGL 360
           CTAALTTLFAKRLLVGHWNVIDVCNGLLGGFAAITSGCAVVEPWAAVICGFVASWVLIGL
Sbjct: 301 CTAALTTLFAKRLLVGHWNVIDVCNGLLGGFAAITSGCAVVEPWAAVICGFVASWVLIGL 360

Query: 361 NKLAEKVSYDDPLEAAQLHGGCGAWGLIFTGLFATKKYVGEVYEVGRPFGLLMGGGGKLL 420
           NKLAE+VSYDDPLEAAQLHGGCGAWGLIFTGLFATKKYVGEVY+ GRP+GLLMGGG KLL
Sbjct: 361 NKLAERVSYDDPLEAAQLHGGCGAWGLIFTGLFATKKYVGEVYQAGRPYGLLMGGGRKLL 420

Query: 421 AAQVVEIVAIIGWVSVTMGGLFYGLKKVKLLRISREDEMAGMDLTRHGGFAYVYNDEEDL 473
           AAQVVEIVAI+GWVSVTMGGLFYGL K KLLRISREDEMAGMDLTRHGGFAYVYNDEEDL
Sbjct: 421 AAQVVEIVAIVGWVSVTMGGLFYGLNKAKLLRISREDEMAGMDLTRHGGFAYVYNDEEDL 480

BLAST of Lsi11G009060 vs. NCBI nr
Match: XP_008441797.1 (PREDICTED: ammonium transporter 1 member 2 [Cucumis melo] >KAA0040459.1 ammonium transporter 1 member 2 [Cucumis melo var. makuwa] >TYK28647.1 ammonium transporter 1 member 2 [Cucumis melo var. makuwa])

HSP 1 Score: 874.8 bits (2259), Expect = 3.4e-250
Identity = 443/511 (86.69%), Postives = 463/511 (90.61%), Query Frame = 0

Query: 1   MSTLSCQPSDLVPLLGSSATTNSTAIAEFLCSRFTTIATTFSSTTYAVDNTYLLFSAYLV 60
           M+TLSCQPSDL+PLLGSSATTNSTA+AEFLCSRFTTIATTFSSTTYA+DNTYLLFSAYLV
Sbjct: 1   MATLSCQPSDLIPLLGSSATTNSTAVAEFLCSRFTTIATTFSSTTYAIDNTYLLFSAYLV 60

Query: 61  FAMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLSYYLFGFAFAF------------- 120
           FAMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLSYYLFGFAFAF             
Sbjct: 61  FAMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLSYYLFGFAFAFGAPSNSFIGRHFF 120

Query: 121 --------------------------GITSGSIAERTQFVAYLIYSTILTGFIYPIVSHW 180
                                     GITSGSIAERT+FVAYLIYSTILTGFIYPIVSHW
Sbjct: 121 DLSSVPNPSTDYSFFLYQWAFAIAAAGITSGSIAERTRFVAYLIYSTILTGFIYPIVSHW 180

Query: 181 FWSADGWASPTRPDNLIFNSGAIDFAGSGVVHMVGGIAGLWGALIEGPRIGRFDRSGRSV 240
           FWSADGWASPTR +NL+FNSGAIDFAGSGVVHMVGG+AGLWGALIEGPRIGRFDRSGRSV
Sbjct: 181 FWSADGWASPTRSNNLLFNSGAIDFAGSGVVHMVGGVAGLWGALIEGPRIGRFDRSGRSV 240

Query: 241 ALRGHSASLVVLGSFLLWFGWYGFNPGSFLTISKSYNDGRPYYGQWSAIGRTGVTTTLAG 300
           ALRGHSASLVVLGSFLLWFGWYGFNPGSFLTISKSYNDGRPYYGQWSAIGRTGVTTTLAG
Sbjct: 241 ALRGHSASLVVLGSFLLWFGWYGFNPGSFLTISKSYNDGRPYYGQWSAIGRTGVTTTLAG 300

Query: 301 CTAALTTLFAKRLLVGHWNVIDVCNGLLGGFAAITSGCAVVEPWAAVICGFVASWVLIGL 360
           CTAALTTLFAKRLLVGHWNVIDVCNGLLGGFAAITSGCAVVEPWAAVICGFVASWVLIG 
Sbjct: 301 CTAALTTLFAKRLLVGHWNVIDVCNGLLGGFAAITSGCAVVEPWAAVICGFVASWVLIGF 360

Query: 361 NKLAEKVSYDDPLEAAQLHGGCGAWGLIFTGLFATKKYVGEVYEVGRPFGLLMGGGGKLL 420
           NKLAE++S+DDPLEAAQLHGGCGAWGLIFTGLFATKKYVGEVY+VGRP+GLLMGGG +LL
Sbjct: 361 NKLAERLSFDDPLEAAQLHGGCGAWGLIFTGLFATKKYVGEVYQVGRPYGLLMGGGWRLL 420

Query: 421 AAQVVEIVAIIGWVSVTMGGLFYGLKKVKLLRISREDEMAGMDLTRHGGFAYVYNDEEDL 473
           AAQVVEIVAI GWVS+TMGGLFYGL K KLLRISREDEMAGMDLTRHGGFAYVYNDEED+
Sbjct: 421 AAQVVEIVAIFGWVSLTMGGLFYGLMKGKLLRISREDEMAGMDLTRHGGFAYVYNDEEDI 480

BLAST of Lsi11G009060 vs. NCBI nr
Match: KAE8651722.1 (hypothetical protein Csa_006722 [Cucumis sativus])

HSP 1 Score: 869.4 bits (2245), Expect = 1.4e-248
Identity = 439/508 (86.42%), Postives = 460/508 (90.55%), Query Frame = 0

Query: 1   MSTLSCQPSDLVPLLGSSATTNSTAIAEFLCSRFTTIATTFSSTTYAVDNTYLLFSAYLV 60
           M+TLSCQPSDL+PLLGSSATTNSTA+AEFLCSRFTTIA+TFSSTTYAVDN YLLFSAYLV
Sbjct: 39  MATLSCQPSDLIPLLGSSATTNSTAVAEFLCSRFTTIASTFSSTTYAVDNNYLLFSAYLV 98

Query: 61  FAMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLSYYLFGFAFAF------------- 120
           FAMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLSYYLFGFAFAF             
Sbjct: 99  FAMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLSYYLFGFAFAFGAPSNSFIGRHFF 158

Query: 121 --------------------------GITSGSIAERTQFVAYLIYSTILTGFIYPIVSHW 180
                                     GITSGSIAERTQFVAYLIYST+LTGFIYPIVSHW
Sbjct: 159 DLSSVPNPSTDYSFFLYQWAFAIATAGITSGSIAERTQFVAYLIYSTVLTGFIYPIVSHW 218

Query: 181 FWSADGWASPTRPDNLIFNSGAIDFAGSGVVHMVGGIAGLWGALIEGPRIGRFDRSGRSV 240
           FWSADGWASPTRP+NL+FNSGAIDFAGSGVVHMVGG+AGLWG+LIEGPRIGRFDRSGRSV
Sbjct: 219 FWSADGWASPTRPNNLLFNSGAIDFAGSGVVHMVGGVAGLWGSLIEGPRIGRFDRSGRSV 278

Query: 241 ALRGHSASLVVLGSFLLWFGWYGFNPGSFLTISKSYNDGRPYYGQWSAIGRTGVTTTLAG 300
           ALRGHSASLVVLGSFLLWFGWYGFNPGSFLTISKSYNDGRPYYGQWSAIGRTGVTTTLAG
Sbjct: 279 ALRGHSASLVVLGSFLLWFGWYGFNPGSFLTISKSYNDGRPYYGQWSAIGRTGVTTTLAG 338

Query: 301 CTAALTTLFAKRLLVGHWNVIDVCNGLLGGFAAITSGCAVVEPWAAVICGFVASWVLIGL 360
           CTAALTTLFAKRLLVGHWNVIDVCNGLLGGFAAITSGCAVVEPWAAVICGFVASWVLIG 
Sbjct: 339 CTAALTTLFAKRLLVGHWNVIDVCNGLLGGFAAITSGCAVVEPWAAVICGFVASWVLIGF 398

Query: 361 NKLAEKVSYDDPLEAAQLHGGCGAWGLIFTGLFATKKYVGEVYEVGRPFGLLMGGGGKLL 420
           NKLAE++S+DDPLEAAQLHGGCGAWGLIFTGLFATKKYVGEVY++GRP+GLLMGGG KLL
Sbjct: 399 NKLAERLSFDDPLEAAQLHGGCGAWGLIFTGLFATKKYVGEVYQLGRPYGLLMGGGWKLL 458

Query: 421 AAQVVEIVAIIGWVSVTMGGLFYGLKKVKLLRISREDEMAGMDLTRHGGFAYVYNDEEDL 470
           AAQVVEIVAI GWVS+TMGGLFYGL K KLLRISREDEMAGMDLTRHGGFAYVYNDEED 
Sbjct: 459 AAQVVEIVAIFGWVSLTMGGLFYGLMKGKLLRISREDEMAGMDLTRHGGFAYVYNDEEDF 518

BLAST of Lsi11G009060 vs. NCBI nr
Match: XP_004152863.1 (ammonium transporter 1 member 2 [Cucumis sativus])

HSP 1 Score: 869.4 bits (2245), Expect = 1.4e-248
Identity = 439/508 (86.42%), Postives = 460/508 (90.55%), Query Frame = 0

Query: 1   MSTLSCQPSDLVPLLGSSATTNSTAIAEFLCSRFTTIATTFSSTTYAVDNTYLLFSAYLV 60
           M+TLSCQPSDL+PLLGSSATTNSTA+AEFLCSRFTTIA+TFSSTTYAVDN YLLFSAYLV
Sbjct: 1   MATLSCQPSDLIPLLGSSATTNSTAVAEFLCSRFTTIASTFSSTTYAVDNNYLLFSAYLV 60

Query: 61  FAMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLSYYLFGFAFAF------------- 120
           FAMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLSYYLFGFAFAF             
Sbjct: 61  FAMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLSYYLFGFAFAFGAPSNSFIGRHFF 120

Query: 121 --------------------------GITSGSIAERTQFVAYLIYSTILTGFIYPIVSHW 180
                                     GITSGSIAERTQFVAYLIYST+LTGFIYPIVSHW
Sbjct: 121 DLSSVPNPSTDYSFFLYQWAFAIATAGITSGSIAERTQFVAYLIYSTVLTGFIYPIVSHW 180

Query: 181 FWSADGWASPTRPDNLIFNSGAIDFAGSGVVHMVGGIAGLWGALIEGPRIGRFDRSGRSV 240
           FWSADGWASPTRP+NL+FNSGAIDFAGSGVVHMVGG+AGLWG+LIEGPRIGRFDRSGRSV
Sbjct: 181 FWSADGWASPTRPNNLLFNSGAIDFAGSGVVHMVGGVAGLWGSLIEGPRIGRFDRSGRSV 240

Query: 241 ALRGHSASLVVLGSFLLWFGWYGFNPGSFLTISKSYNDGRPYYGQWSAIGRTGVTTTLAG 300
           ALRGHSASLVVLGSFLLWFGWYGFNPGSFLTISKSYNDGRPYYGQWSAIGRTGVTTTLAG
Sbjct: 241 ALRGHSASLVVLGSFLLWFGWYGFNPGSFLTISKSYNDGRPYYGQWSAIGRTGVTTTLAG 300

Query: 301 CTAALTTLFAKRLLVGHWNVIDVCNGLLGGFAAITSGCAVVEPWAAVICGFVASWVLIGL 360
           CTAALTTLFAKRLLVGHWNVIDVCNGLLGGFAAITSGCAVVEPWAAVICGFVASWVLIG 
Sbjct: 301 CTAALTTLFAKRLLVGHWNVIDVCNGLLGGFAAITSGCAVVEPWAAVICGFVASWVLIGF 360

Query: 361 NKLAEKVSYDDPLEAAQLHGGCGAWGLIFTGLFATKKYVGEVYEVGRPFGLLMGGGGKLL 420
           NKLAE++S+DDPLEAAQLHGGCGAWGLIFTGLFATKKYVGEVY++GRP+GLLMGGG KLL
Sbjct: 361 NKLAERLSFDDPLEAAQLHGGCGAWGLIFTGLFATKKYVGEVYQLGRPYGLLMGGGWKLL 420

Query: 421 AAQVVEIVAIIGWVSVTMGGLFYGLKKVKLLRISREDEMAGMDLTRHGGFAYVYNDEEDL 470
           AAQVVEIVAI GWVS+TMGGLFYGL K KLLRISREDEMAGMDLTRHGGFAYVYNDEED 
Sbjct: 421 AAQVVEIVAIFGWVSLTMGGLFYGLMKGKLLRISREDEMAGMDLTRHGGFAYVYNDEEDF 480

BLAST of Lsi11G009060 vs. NCBI nr
Match: XP_008441800.1 (PREDICTED: ammonium transporter 1 member 2-like [Cucumis melo] >KAA0040463.1 ammonium transporter 1 member 2-like [Cucumis melo var. makuwa] >TYK28652.1 ammonium transporter 1 member 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 860.9 bits (2223), Expect = 5.1e-246
Identity = 436/511 (85.32%), Postives = 455/511 (89.04%), Query Frame = 0

Query: 1   MSTLSCQPSDLVPLLGSSATTNSTAIAEFLCSRFTTIATTFSSTTYAVDNTYLLFSAYLV 60
           M+TLSCQPSDLVPLLGSSAT NSTAIAEFLCSRFTTIATTFSSTTYAVDNTYLLFSAYLV
Sbjct: 1   MATLSCQPSDLVPLLGSSATANSTAIAEFLCSRFTTIATTFSSTTYAVDNTYLLFSAYLV 60

Query: 61  FAMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLSYYLFGFAFAF------------- 120
           FAMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLSYYLFGFAFAF             
Sbjct: 61  FAMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLSYYLFGFAFAFGAPSNSFIGRHFF 120

Query: 121 --------------------------GITSGSIAERTQFVAYLIYSTILTGFIYPIVSHW 180
                                     GITSGSIAERTQFVAYLIYS+ILTGF+YPIVSHW
Sbjct: 121 GLSNFPNPSTDYSFFLYQWAFAIAAAGITSGSIAERTQFVAYLIYSSILTGFVYPIVSHW 180

Query: 181 FWSADGWASPTRPDNLIFNSGAIDFAGSGVVHMVGGIAGLWGALIEGPRIGRFDRSGRSV 240
           FWS+DGWASPTRP NL+FNSGAIDFAGSGVVHMVGGIAGLWGALIEGPRIGRFDRSGRSV
Sbjct: 181 FWSSDGWASPTRPSNLLFNSGAIDFAGSGVVHMVGGIAGLWGALIEGPRIGRFDRSGRSV 240

Query: 241 ALRGHSASLVVLGSFLLWFGWYGFNPGSFLTISKSYNDGRPYYGQWSAIGRTGVTTTLAG 300
           ALRGHSASLVVLGSFLLWFGWYGFNPGSFLTISKSYNDGRPYYGQWSAIGRTGVTTTLAG
Sbjct: 241 ALRGHSASLVVLGSFLLWFGWYGFNPGSFLTISKSYNDGRPYYGQWSAIGRTGVTTTLAG 300

Query: 301 CTAALTTLFAKRLLVGHWNVIDVCNGLLGGFAAITSGCAVVEPWAAVICGFVASWVLIGL 360
           CTAALTTLF KRLLVGHWNVIDVCNGLLGGFAAITSGCAVVEPWAAVICGFVASWVLI  
Sbjct: 301 CTAALTTLFTKRLLVGHWNVIDVCNGLLGGFAAITSGCAVVEPWAAVICGFVASWVLISF 360

Query: 361 NKLAEKVSYDDPLEAAQLHGGCGAWGLIFTGLFATKKYVGEVYEVGRPFGLLMGGGGKLL 420
           NKLAEKV+YDDPLEAAQLHGGCGAWGLIFTGLFA K+YV EVY+ GRP+GL MGGGG+LL
Sbjct: 361 NKLAEKVNYDDPLEAAQLHGGCGAWGLIFTGLFARKEYVAEVYQAGRPYGLFMGGGGRLL 420

Query: 421 AAQVVEIVAIIGWVSVTMGGLFYGLKKVKLLRISREDEMAGMDLTRHGGFAYVYNDEEDL 473
           AAQ++EI+AIIGWVSVTMGGLFY LKKVKLLRISREDEMAGMDLTRHGGFAYVYNDE+D 
Sbjct: 421 AAQLIEILAIIGWVSVTMGGLFYALKKVKLLRISREDEMAGMDLTRHGGFAYVYNDEDDP 480

BLAST of Lsi11G009060 vs. TAIR 10
Match: AT1G64780.1 (ammonium transporter 1;2 )

HSP 1 Score: 728.4 bits (1879), Expect = 3.7e-210
Identity = 371/503 (73.76%), Postives = 407/503 (80.91%), Query Frame = 0

Query: 2   STLSCQPSDLVPLLGSSA-TTNSTAIAEFLCSRFTTIATTFSSTTYAVDNTYLLFSAYLV 61
           +T +C   DL  LL SS+ +T+S A A FLCS+ + I+   S TTYAVDNTYLLFSAYLV
Sbjct: 4   ATTTCSAVDLSALLSSSSNSTSSLAAATFLCSQISNISNKLSDTTYAVDNTYLLFSAYLV 63

Query: 62  FAMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLSYYLFGFAFAF------------- 121
           FAMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAG +SYYLFGFAFAF             
Sbjct: 64  FAMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGAISYYLFGFAFAFGTPSNGFIGRHHS 123

Query: 122 -----------------------------GITSGSIAERTQFVAYLIYSTILTGFIYPIV 181
                                        GITSGSIAERTQFVAYLIYST LTGF+YP V
Sbjct: 124 FFALSSYPERPGSDFSFFLYQWAFAIAAAGITSGSIAERTQFVAYLIYSTFLTGFVYPTV 183

Query: 182 SHWFWSADGWASPTRPD-NLIFNSGAIDFAGSGVVHMVGGIAGLWGALIEGPRIGRFDRS 241
           SHWFWS+DGWAS +R D NL+F SGAIDFAGSGVVHMVGGIAGL GAL+EGPRIGRFDRS
Sbjct: 184 SHWFWSSDGWASASRSDNNLLFGSGAIDFAGSGVVHMVGGIAGLCGALVEGPRIGRFDRS 243

Query: 242 GRSVALRGHSASLVVLGSFLLWFGWYGFNPGSFLTISKSYNDGRPYYGQWSAIGRTGVTT 301
           GRSVALRGHSASLVVLG+FLLWFGWYGFNPGSFLTI K Y+  RPYYGQWSA+GRT VTT
Sbjct: 244 GRSVALRGHSASLVVLGTFLLWFGWYGFNPGSFLTILKGYDKSRPYYGQWSAVGRTAVTT 303

Query: 302 TLAGCTAALTTLFAKRLLVGHWNVIDVCNGLLGGFAAITSGCAVVEPWAAVICGFVASWV 361
           TL+GCTAALTTLF+KRLL GHWNVIDVCNGLLGGFAAITSGCAVVEPWAA++CGFVASWV
Sbjct: 304 TLSGCTAALTTLFSKRLLAGHWNVIDVCNGLLGGFAAITSGCAVVEPWAAIVCGFVASWV 363

Query: 362 LIGLNKLAEKVSYDDPLEAAQLHGGCGAWGLIFTGLFATKKYVGEVYEVGRPFGLLMGGG 421
           LIG N LA+K+ YDDPLEAAQLHGGCGAWGLIFTGLFA K+YV E+Y   RP+GL MGGG
Sbjct: 364 LIGFNLLAKKLKYDDPLEAAQLHGGCGAWGLIFTGLFARKEYVNEIYSGDRPYGLFMGGG 423

Query: 422 GKLLAAQVVEIVAIIGWVSVTMGGLFYGLKKVKLLRISREDEMAGMDLTRHGGFAYVYND 461
           GKLLAAQ+V+I+ I+GWV+VTMG LFYGL K+ LLRIS EDEMAGMD+TRHGGFAY YND
Sbjct: 424 GKLLAAQIVQIIVIVGWVTVTMGPLFYGLHKMNLLRISAEDEMAGMDMTRHGGFAYAYND 483

BLAST of Lsi11G009060 vs. TAIR 10
Match: AT4G28700.1 (ammonium transporter 1;4 )

HSP 1 Score: 669.5 bits (1726), Expect = 2.0e-192
Identity = 340/496 (68.55%), Postives = 390/496 (78.63%), Query Frame = 0

Query: 2   STLSCQPSDLVPLL-GSSATTNSTAIAEFLCSRFTTIATTFSSTTYAVDNTYLLFSAYLV 61
           S LSC  SDL+PLL G +  T + A AE++C RF T+A  F+   YA+DNTYLLFSAYLV
Sbjct: 3   SALSCSASDLIPLLSGGANATAAAAAAEYICGRFDTVAGKFTDAAYAIDNTYLLFSAYLV 62

Query: 62  FAMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLSYYLFGFAFAF------------- 121
           FAMQLGFAMLCAGSVRAKNTMNIMLTNV+DAAAGGL YYLFGFAFAF             
Sbjct: 63  FAMQLGFAMLCAGSVRAKNTMNIMLTNVIDAAAGGLFYYLFGFAFAFGSPSNGFIGKHFF 122

Query: 122 --------------------------GITSGSIAERTQFVAYLIYSTILTGFIYPIVSHW 181
                                     GITSGSIAERTQFVAYLIYS+ LTG +YPIVSHW
Sbjct: 123 GMYDFPQPTFDYPYFLYQWTFAIAAAGITSGSIAERTQFVAYLIYSSFLTGLVYPIVSHW 182

Query: 182 FWSADGWASPTRPDNLIFNSGAIDFAGSGVVHMVGGIAGLWGALIEGPRIGRFDRSGRSV 241
           FWS+DGWASP R +NL+F SG IDFAGSGVVHMVGGIAGLWGALIEGPRIGRF   G+ V
Sbjct: 183 FWSSDGWASPARSENLLFQSGVIDFAGSGVVHMVGGIAGLWGALIEGPRIGRFGVGGKPV 242

Query: 242 ALRGHSASLVVLGSFLLWFGWYGFNPGSFLTISKSYND--GRPYYGQWSAIGRTGVTTTL 301
            LRGHSA+LVVLG+FLLWFGWYGFNPGSF TI K+Y +  G  +YGQWSA+GRT VTTTL
Sbjct: 243 TLRGHSATLVVLGTFLLWFGWYGFNPGSFATIFKAYGETPGSSFYGQWSAVGRTAVTTTL 302

Query: 302 AGCTAALTTLFAKRLLVGHWNVIDVCNGLLGGFAAITSGCAVVEPWAAVICGFVASWVLI 361
           AGCTAALTTLF KRL+ G+WNV DVCNGLLGGFAAITSGC+VVEPWAA++CGFVA+WVL+
Sbjct: 303 AGCTAALTTLFGKRLIDGYWNVTDVCNGLLGGFAAITSGCSVVEPWAALVCGFVAAWVLM 362

Query: 362 GLNKLAEKVSYDDPLEAAQLHGGCGAWGLIFTGLFATKKYVGEVY--EVGRPFGLLMGGG 421
           G N+LAEK+ +DDPLEAAQLHGGCGAWG+IFTGLFA K+Y+ E++  +  RPFGLLMGGG
Sbjct: 363 GCNRLAEKLQFDDPLEAAQLHGGCGAWGIIFTGLFAEKRYIAEIFGGDPNRPFGLLMGGG 422

Query: 422 GKLLAAQVVEIVAIIGWVSVTMGGLFYGLKKVKLLRISREDEMAGMDLTRHGGFAYVYND 454
           G+LLAA VV+I+ I GWVSVTMG LF+ L K+KLLRI  EDE+AG+D T HGG AY+Y +
Sbjct: 423 GRLLAAHVVQILVITGWVSVTMGTLFFILHKLKLLRIPAEDEIAGVDPTSHGGLAYMYTE 482

BLAST of Lsi11G009060 vs. TAIR 10
Match: AT4G13510.1 (ammonium transporter 1;1 )

HSP 1 Score: 662.1 bits (1707), Expect = 3.2e-190
Identity = 341/505 (67.52%), Postives = 388/505 (76.83%), Query Frame = 0

Query: 4   LSCQPSDLVPLLGSSATTNSTAIAEFLCSRFTTIATTFSSTTYAVDNTYLLFSAYLVFAM 63
           +SC  +DL  LLG     N+TA A ++C +   +   F  T +A+DNTYLLFSAYLVF+M
Sbjct: 1   MSCSATDLAVLLG----PNATAAANYICGQLGDVNNKFIDTAFAIDNTYLLFSAYLVFSM 60

Query: 64  QLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLSYYLFGFAFAF---------------- 123
           QLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGGL YYLFG+AFAF                
Sbjct: 61  QLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLFYYLFGYAFAFGSPSNGFIGKHYFGLK 120

Query: 124 -----------------------GITSGSIAERTQFVAYLIYSTILTGFIYPIVSHWFWS 183
                                  GITSGSIAERTQFVAYLIYS+ LTGF+YP+VSHWFWS
Sbjct: 121 DIPTASADYSNFLYQWAFAIAAAGITSGSIAERTQFVAYLIYSSFLTGFVYPVVSHWFWS 180

Query: 184 ADGWASPTRPD-NLIFNSGAIDFAGSGVVHMVGGIAGLWGALIEGPRIGRFDRSGRSVAL 243
            DGWASP R D +L+F++GAIDFAGSGVVHMVGGIAGLWGALIEGPR+GRFD  GR++AL
Sbjct: 181 VDGWASPFRTDGDLLFSTGAIDFAGSGVVHMVGGIAGLWGALIEGPRLGRFDNGGRAIAL 240

Query: 244 RGHSASLVVLGSFLLWFGWYGFNPGSFLTISKSYNDGRPYYGQWSAIGRTGVTTTLAGCT 303
           RGHSASLVVLG+FLLWFGWYGFNPGSF  I  +Y  G  Y GQWSA+GRT VTTTLAGCT
Sbjct: 241 RGHSASLVVLGTFLLWFGWYGFNPGSFNKILVTYETG-TYNGQWSAVGRTAVTTTLAGCT 300

Query: 304 AALTTLFAKRLLVGHWNVIDVCNGLLGGFAAITSGCAVVEPWAAVICGFVASWVLIGLNK 363
           AALTTLF KRLL GHWNV DVCNGLLGGFAAIT GC+VVEPWAA+ICGFVA+ VL+G NK
Sbjct: 301 AALTTLFGKRLLSGHWNVTDVCNGLLGGFAAITGGCSVVEPWAAIICGFVAALVLLGCNK 360

Query: 364 LAEKVSYDDPLEAAQLHGGCGAWGLIFTGLFATKKYVGEVY--EVGRPFGLLMGGGGKLL 423
           LAEK+ YDDPLEAAQLHGGCGAWGLIFT LFA +KY+ ++Y  + GRP GL MGGGGKLL
Sbjct: 361 LAEKLKYDDPLEAAQLHGGCGAWGLIFTALFAQEKYLNQIYGNKPGRPHGLFMGGGGKLL 420

Query: 424 AAQVVEIVAIIGWVSVTMGGLFYGLKKVKLLRISREDEMAGMDLTRHGGFAYVYNDEEDL 467
            AQ+++I+ I GWVS TMG LF+ LKK+KLLRIS EDEMAGMD+TRHGGFAY+Y D++  
Sbjct: 421 GAQLIQIIVITGWVSATMGTLFFILKKMKLLRISSEDEMAGMDMTRHGGFAYMYFDDD-- 480

BLAST of Lsi11G009060 vs. TAIR 10
Match: AT3G24300.1 (ammonium transporter 1;3 )

HSP 1 Score: 649.4 bits (1674), Expect = 2.2e-186
Identity = 330/483 (68.32%), Postives = 377/483 (78.05%), Query Frame = 0

Query: 4   LSCQPSDLVPLLGSSATTNSTAIAEFLCSRFTTIATTFSSTTYAVDNTYLLFSAYLVFAM 63
           ++C  +DL  LLG     N+TA A+++C +  T+   F+   +A+DNTYLLFSAYLVFAM
Sbjct: 5   ITCSAADLATLLG----PNATAAADYICGQLGTVNNKFTDAAFAIDNTYLLFSAYLVFAM 64

Query: 64  QLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLSYYLFGFAFAF---------------- 123
           QLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGGL YYLFG+AFAF                
Sbjct: 65  QLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLFYYLFGYAFAFGGSSEGFIGRHNFALR 124

Query: 124 -----------------------GITSGSIAERTQFVAYLIYSTILTGFIYPIVSHWFWS 183
                                  GITSGSIAERTQFVAYLIYS+ LTGF+YP+VSHWFWS
Sbjct: 125 DFPTPTADYSFFLYQWAFAIAAAGITSGSIAERTQFVAYLIYSSFLTGFVYPVVSHWFWS 184

Query: 184 ADGWASPTR-PDNLIFNSGAIDFAGSGVVHMVGGIAGLWGALIEGPRIGRFDRSGRSVAL 243
            DGWASP R  D+ +F++GAIDFAGSGVVHMVGGIAGLWGALIEGPR GRF++ GR++AL
Sbjct: 185 PDGWASPFRSADDRLFSTGAIDFAGSGVVHMVGGIAGLWGALIEGPRRGRFEKGGRAIAL 244

Query: 244 RGHSASLVVLGSFLLWFGWYGFNPGSFLTISKSYNDGRPYYGQWSAIGRTGVTTTLAGCT 303
           RGHSASLVVLG+FLLWFGWYGFNPGSF  I   YN G   YGQWS IGRT V TTL+GCT
Sbjct: 245 RGHSASLVVLGTFLLWFGWYGFNPGSFTKILVPYNSG-SNYGQWSGIGRTAVNTTLSGCT 304

Query: 304 AALTTLFAKRLLVGHWNVIDVCNGLLGGFAAITSGCAVVEPWAAVICGFVASWVLIGLNK 363
           AALTTLF KRLL GHWNV DVCNGLLGGFAAIT+GC+VVEPWAA++CGF+AS VLIG NK
Sbjct: 305 AALTTLFGKRLLSGHWNVTDVCNGLLGGFAAITAGCSVVEPWAAIVCGFMASVVLIGCNK 364

Query: 364 LAEKVSYDDPLEAAQLHGGCGAWGLIFTGLFATKKYVGEVYEV--GRPFGLLMGGGGKLL 423
           LAE V YDDPLEAAQLHGGCGAWGLIF GLFA +KY+ EVY    GRP+GL MGGGGKLL
Sbjct: 365 LAELVQYDDPLEAAQLHGGCGAWGLIFVGLFAKEKYLNEVYGATPGRPYGLFMGGGGKLL 424

Query: 424 AAQVVEIVAIIGWVSVTMGGLFYGLKKVKLLRISREDEMAGMDLTRHGGFAYVYNDEEDL 445
            AQ+V+I+ I+GWVS TMG LF+ LK++ LLRIS + EM GMD+TRHGGFAY+Y+D +D 
Sbjct: 425 GAQLVQILVIVGWVSATMGTLFFILKRLNLLRISEQHEMQGMDMTRHGGFAYIYHDNDDE 482

BLAST of Lsi11G009060 vs. TAIR 10
Match: AT3G24290.1 (ammonium transporter 1;5 )

HSP 1 Score: 642.9 bits (1657), Expect = 2.0e-184
Identity = 326/478 (68.20%), Postives = 370/478 (77.41%), Query Frame = 0

Query: 4   LSCQPSDLVPLLGSSATTNSTAIAEFLCSRFTTIATTFSSTTYAVDNTYLLFSAYLVFAM 63
           ++C  +DL  LLG     N+TA A+++C +  ++   F+   YA+DNTYLLFSAYLVFAM
Sbjct: 5   ITCSAADLSALLG----PNATAAADYICGQLGSVNNKFTDAAYAIDNTYLLFSAYLVFAM 64

Query: 64  QLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLSYYLFGFAFAF---------------- 123
           QLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGGL YYLFG+AFAF                
Sbjct: 65  QLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLFYYLFGYAFAFGESSDGFIGRHNFGLQ 124

Query: 124 -----------------------GITSGSIAERTQFVAYLIYSTILTGFIYPIVSHWFWS 183
                                  GITSGSIAERT+FVAYLIYS+ LTGF+YP+VSHWFWS
Sbjct: 125 NFPTLTSDYSFFLYQWAFAIAAAGITSGSIAERTKFVAYLIYSSFLTGFVYPVVSHWFWS 184

Query: 184 ADGWASPTRPDNLIFNSGAIDFAGSGVVHMVGGIAGLWGALIEGPRIGRFDRSGRSVALR 243
            DGWASP R ++ +F +GAIDFAGSGVVHMVGGIAGLWGALIEGPRIGRF   G ++ALR
Sbjct: 185 PDGWASPFRSEDRLFGTGAIDFAGSGVVHMVGGIAGLWGALIEGPRIGRFPDGGHAIALR 244

Query: 244 GHSASLVVLGSFLLWFGWYGFNPGSFLTISKSYNDGRPYYGQWSAIGRTGVTTTLAGCTA 303
           GHSASLVVLG+FLLWFGWYGFNPGSF  I   YN G   YGQWS IGRT VTTTL+GCTA
Sbjct: 245 GHSASLVVLGTFLLWFGWYGFNPGSFTKILIPYNSG-SNYGQWSGIGRTAVTTTLSGCTA 304

Query: 304 ALTTLFAKRLLVGHWNVIDVCNGLLGGFAAITSGCAVVEPWAAVICGFVASWVLIGLNKL 363
           ALTTLF KRLL GHWNV DVCNGLLGGFAAIT+GC+VV+PWAA++CGFVAS VLIG NKL
Sbjct: 305 ALTTLFGKRLLSGHWNVTDVCNGLLGGFAAITAGCSVVDPWAAIVCGFVASLVLIGCNKL 364

Query: 364 AEKVSYDDPLEAAQLHGGCGAWGLIFTGLFATKKYVGEVY--EVGRPFGLLMGGGGKLLA 423
           AE + YDDPLEAAQLHGGCGAWGLIF GLFA +KY+ EVY    GR +GL MGGGGKLL 
Sbjct: 365 AELLKYDDPLEAAQLHGGCGAWGLIFVGLFAKEKYINEVYGASPGRHYGLFMGGGGKLLG 424

Query: 424 AQVVEIVAIIGWVSVTMGGLFYGLKKVKLLRISREDEMAGMDLTRHGGFAYVYNDEED 441
           AQ+V+I+ I+GWVS TMG LF+ LKK+ LLRIS + EM GMDL  HGGFAY+Y+D +D
Sbjct: 425 AQLVQIIVIVGWVSATMGTLFFILKKLNLLRISEQHEMRGMDLAGHGGFAYIYHDNDD 477

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9ZPJ85.2e-20973.76Ammonium transporter 1 member 2 OS=Arabidopsis thaliana OX=3702 GN=AMT1-2 PE=1 S... [more]
O041615.0e-20471.96Ammonium transporter 1 member 2 OS=Solanum lycopersicum OX=4081 GN=AMT1-2 PE=2 S... [more]
Q9SVT82.9e-19168.55Ammonium transporter 1 member 4 OS=Arabidopsis thaliana OX=3702 GN=AMT1-4 PE=1 S... [more]
P541444.6e-18967.52Ammonium transporter 1 member 1 OS=Arabidopsis thaliana OX=3702 GN=AMT1-1 PE=1 S... [more]
Q7XQ123.6e-18667.66Ammonium transporter 1 member 1 OS=Oryza sativa subsp. japonica OX=39947 GN=AMT1... [more]
Match NameE-valueIdentityDescription
A0A5D3DXN71.6e-25086.69Ammonium transporter OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold165... [more]
A0A1S3B4A31.6e-25086.69Ammonium transporter OS=Cucumis melo OX=3656 GN=LOC103485850 PE=3 SV=1[more]
A0A0A0LKM76.9e-24986.42Ammonium transporter OS=Cucumis sativus OX=3659 GN=Csa_2G079100 PE=3 SV=1[more]
A0A5A7TBL12.5e-24685.32Ammonium transporter OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold165... [more]
A0A1S3B4X22.5e-24685.32Ammonium transporter OS=Cucumis melo OX=3656 GN=LOC103485853 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_038890689.12.4e-25689.24ammonium transporter 1 member 2-like [Benincasa hispida][more]
XP_008441797.13.4e-25086.69PREDICTED: ammonium transporter 1 member 2 [Cucumis melo] >KAA0040459.1 ammonium... [more]
KAE8651722.11.4e-24886.42hypothetical protein Csa_006722 [Cucumis sativus][more]
XP_004152863.11.4e-24886.42ammonium transporter 1 member 2 [Cucumis sativus][more]
XP_008441800.15.1e-24685.32PREDICTED: ammonium transporter 1 member 2-like [Cucumis melo] >KAA0040463.1 amm... [more]
Match NameE-valueIdentityDescription
AT1G64780.13.7e-21073.76ammonium transporter 1;2 [more]
AT4G28700.12.0e-19268.55ammonium transporter 1;4 [more]
AT4G13510.13.2e-19067.52ammonium transporter 1;1 [more]
AT3G24300.12.2e-18668.32ammonium transporter 1;3 [more]
AT3G24290.12.0e-18468.20ammonium transporter 1;5 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029020Ammonium/urea transporterGENE3D1.10.3430.10Ammonium transporter AmtB like domainscoord: 110..449
e-value: 3.8E-105
score: 354.1
coord: 31..109
e-value: 7.5E-21
score: 76.3
IPR001905Ammonium transporterTIGRFAMTIGR00836TIGR00836coord: 104..433
e-value: 1.3E-109
score: 364.8
IPR024041Ammonium transporter AmtB-like domainPFAMPF00909Ammonium_transpcoord: 52..108
e-value: 4.8E-14
score: 51.9
coord: 104..433
e-value: 4.6E-106
score: 354.9
NoneNo IPR availablePANTHERPTHR11730AMMONIUM TRANSPORTERcoord: 106..462
coord: 5..108
NoneNo IPR availablePANTHERPTHR11730:SF106AMMONIUM TRANSPORTERcoord: 106..462
coord: 5..108
NoneNo IPR availableSUPERFAMILY111352Ammonium transportercoord: 46..414
IPR018047Ammonium transporter, conserved sitePROSITEPS01219AMMONIUM_TRANSPcoord: 165..190

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi11G009060.1Lsi11G009060.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0072488 ammonium transmembrane transport
biological_process GO:0015843 methylammonium transport
biological_process GO:0015696 ammonium transport
cellular_component GO:0005887 integral component of plasma membrane
cellular_component GO:0016020 membrane
molecular_function GO:0008519 ammonium transmembrane transporter activity